Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

SuperScaffold_520

Orthogroup ID

OG0002632

Physical Location & Seq

Genomic Coordinates Forward (+)

289407 .. 290771

Gene Structure 1365 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom520g00330.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1365 bp

ATGAAGATGGAGAGGCAGGTCAGAATTCCCAATCGAAATCTGACGGTCATCGTTTTCCTCCTTCTCCTTGTTAGTTGTCAGCTTTGCTCCGTCGTCTCCTGGAAGAAAGAAGAGTTCAGGAACTGTAACCAAACCCCATTCTGCAAACGAGCCCGTAGCCGCAAACCCAGCTCCTCCTCCCTTGCCGCCCATGACGTATCTATCTCCGACGGTGACCTCACAGCCAGGCTCGTTCCCTGCGACAAAACCCAGGAAGATGAAGACCAAATACAACTCAAGCCCTTGATTCTCACTCTTTCTGTTTACCAAGATGGGATCATGCTGCTCAAGATCGACGAGGACCCCAAAATCGATCCACCCAAGAAGCGTTTCGAGGTTCCCAATGTGCTTGTGCCGGAGTTTTTTAGCAAGAAGCTGTGGTTGCAGAGGCTTTCAACAGAGACCATCGGCGACGATGTAGGTCCGTCGTCGATCGTCTACTTGTTGGACGGCTACGAGGCGGTGCTCCGGCACGATCCCTTTGAGGTTTACGTGCGCGAAAAGGGAGGTAATCGCGTGGTTTCTATGAATTCTCATGGTTTATTTGATTTTGAGCAATTGAGGGTGAAGAAGGGTGGTGAGGATTGGGAGGAGAGGTTCAAAGGGCATACAGATACGAGGCCTTTCGGCCCGCAATCCATTAGCTTCGACGTGTCTTTTCATGATGCTGATCATGTGTATGGGATTCCTGAGCGTGCTACAAGCTTCGCTCTGAAGCCCACTAGAGGTCCTGGGGTTGAGGAATCTGAACCCTATAGATTGTTTAACTTAGATGTGTTTGAGTACATTCATGATTCCCCATTCGGCCTTTATGGTTCTGTACCCTTAATGATTTCCCACGGCAAGGCGCGCGGGACTTCCGGTTTCTTTTGGTTGAATGCTGCCGAGATGCAGATTGATGTTCTTGGAACCGGCTGGGATGCGGAGTCTGGTATTTCTCTCCCTACGTCACAGAATAGGATCGATACGCATTGGATGAGTGAGGCAGGTATTGTCGATGCCTTCTTTTTCGTAGGACCTGGACCTAAGGATGTAATTCGCCAGTACACGAGTGTAACTGGAACACCTGCAATGCCACAGTTGTTTGCGGTGGCCTATCACCAATGTAGGTGGAATTACCGGGATGAGGAGGATGTTGAGCAAGTTGATGCTAAGTTTGATGAGCACGACATCCCATATGATGTTTTGTGGCTTGATATTGAGCACACAGATGGGAAGAGATACTTTACATGGGATAGAACACTTTTTCCACACCCAGCGGAGATGCAGAGGAAGTTGGCTGCTAAGGGTAGGCACATGGTTACTATTGTGGATCGCTCATATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000902 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006491 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009250 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009826 GO:0009987 GO:0010035 GO:0010038 GO:0012505 GO:0015926 GO:0016043 GO:0016049 GO:0016051 GO:0016787 GO:0016798 GO:0019538 GO:0030243 GO:0030244 GO:0030587 GO:0031288 GO:0032502 GO:0032989 GO:0033692 GO:0033919 GO:0034637 GO:0034645 GO:0036211 GO:0040007 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043687 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048589 GO:0048856 GO:0048869 GO:0050896 GO:0051273 GO:0051274 GO:0051703 GO:0051704 GO:0051707 GO:0060560 GO:0071704 GO:0071840 GO:0090599 GO:0090600 GO:0090702 GO:0098542 GO:0099120 GO:1901135 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00510 ko01100 ko04141 map00510 map01100 map04141

Pfam Domains

Protein Families

DUF5110 Gal_mutarotas_2 Glyco_hydro_31

Protein Analysis

455

Amino Acids

51.95

Weight (kDa)

5.52

Isoelectric Point (pI)

36.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gal_mutarotas_2	PF13802	156 - 313	6.6e-38	Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd	PF01055	369 - 451	3.5e-27	Glycosyl hydrolases family 31 TIM-barrel domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002632)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63840 AT5G63840
fragaria_vesca	FvH4_1g25070
malus_domestica	MD01G1007800.v1.1 MD01G1007900.v1.1 MD15G1135800.v1.1
prunus_persica	Prupe.4G277800_v2.0.a1 Prupe.4G277800_v2.0.a1
pyrus_communis	pycom15g12240 pycom520g00330
rosa_chinensis	RchiOBHm_Chr2g0122711 RchiOBHm_Chr2g0122721
rosa_laevigata	RLG00000018707 RLG00000018709
rosa_multiflora	Rmu_sc0005370.1_g000002 Rmu_sc0008259.1_g000009 Rmu_sc0008259.1_g000014 Rmu_sc0017734.1_g000006 Rmu_sc0026486.1_g000001
rosa_roxburghii	Rroxscaffold_1G00031190 Rroxscaffold_2G00121270 Rroxscaffold_2G00121280 Rroxscaffold_4G00297410 Rroxscaffold_4G00317480 Rroxscaffold_4G00325980 Rroxscaffold_7G00197700 Rroxscaffold_7G00199130
rosa_rugosa	Rorug02G0241800 Rorug02G0241800 Rorug02G0241900 Rorug02G0242000 Rorug02G0242100
rosa_samantha	Rh2AG300300 Rh2BG308200 Rh2CG286400 Rh2CG286900 Rh2DG324500
rosa_wichuraiana	Rw2G023850 Rw2G023860 Rw2G024040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1114
Acc36I	ACCTGC	3 cut(s)	7, 1016, 1114
AccI	GTMKAC	1 cut(s)	477
AccII	CGCG	4 cut(s)	537, 555, 889, 891
AciI	CCGC	8 cut(s)	160, 186, 500, 671, 891, 962, 1127, 1298
AclWI	GGATC	5 cut(s)	323, 347, 509, 1007, 1359
AcsI	RAATTY	2 cut(s)	24, 568
AcuI	CTGAAG	1 cut(s)	773
AfaI	GTAC	3 cut(s)	824, 861, 1085
AfiI	CCNNNNNNNGG	3 cut(s)	764, 883, 1165
AflIII	ACRYGT	1 cut(s)	690
AgsI	TTSAA	3 cut(s)	435, 640, 916
AjiI	CACGTC	1 cut(s)	691
AjnI	CCWGG	5 cut(s)	98, 224, 249, 769, 1057
AjuI	GAANNNNNNNTTGG	2 cut(s)	353, 385
AloI	GAACNNNNNNTCC	4 cut(s)	620, 652, 1270, 1302
AluBI	AGCT	5 cut(s)	82, 171, 415, 684, 744
AluI	AGCT	5 cut(s)	82, 171, 415, 684, 744
Alw21I	GWGCWC	3 cut(s)	507, 1206, 1245
Alw26I	GTCTC	2 cut(s)	100, 434
AlwI	GGATC	5 cut(s)	323, 347, 509, 1007, 1359
AlwNI	CAGNNNCTG	1 cut(s)	123
AoxI	GGCC	4 cut(s)	659, 667, 844, 1131
ApeKI	GCWGC	3 cut(s)	322, 920, 1319
ApoI	RAATTY	2 cut(s)	24, 568
Asp700I	GAANNNNTTC	1 cut(s)	368
AspLEI	GCGC	3 cut(s)	537, 889, 891
AspS9I	GGNCC	6 cut(s)	340, 461, 668, 767, 1055, 1061
AsuC2I	CCSGG	1 cut(s)	1160
AsuHPI	GGTGA	4 cut(s)	224, 616, 629, 1130
AvaII	GGWCC	5 cut(s)	340, 461, 767, 1055, 1061
AxyI	CCTNAGG	1 cut(s)	1065
BanII	GRGCYC	1 cut(s)	154
BarI	GAAGNNNNNNTAC	2 cut(s)	252, 284
BauI	CACGAG	1 cut(s)	1087
BbsI	GAAGAC	1 cut(s)	267
Bbv12I	GWGCWC	3 cut(s)	507, 1206, 1245
BbvI	GCAGC	3 cut(s)	309, 907, 1306
BccI	CCATC	3 cut(s)	305, 452, 1244
BceAI	ACGGC	2 cut(s)	505, 895
BciT130I	CCWGG	5 cut(s)	100, 226, 251, 771, 1059
BclI	TGATCA	1 cut(s)	709
BcnI	CCSGG	1 cut(s)	1160
BcoDI	GTCTC	2 cut(s)	100, 434
BfaI	CTAG	1 cut(s)	762
BfmI	CTRYAG	1 cut(s)	793
BfuAI	ACCTGC	3 cut(s)	7, 1016, 1114
BisI	GCNGC	5 cut(s)	160, 186, 323, 921, 1320
BlsI	GCNGC	5 cut(s)	161, 187, 324, 922, 1321
Bme1390I	CCNGG	6 cut(s)	100, 226, 251, 771, 1059, 1160
Bme18I	GGWCC	5 cut(s)	340, 461, 767, 1055, 1061
BmgBI	CACGTC	1 cut(s)	691
BmgT120I	GGNCC	6 cut(s)	340, 461, 668, 767, 1055, 1061
BmiI	GGNNCC	3 cut(s)	342, 378, 949
BmrFI	CCNGG	6 cut(s)	100, 226, 251, 771, 1059, 1160
BmsI	GCATC	6 cut(s)	694, 918, 949, 1027, 1177, 1293
BpiI	GAAGAC	1 cut(s)	267
Bpu10I	CCTNAGC	2 cut(s)	729, 1323
BpuEI	CTTGAG	2 cut(s)	260, 311
BpuMI	CCSGG	1 cut(s)	1160
Bsa29I	ATCGAT	2 cut(s)	351, 1002
BsaAI	YACGTR	1 cut(s)	532
BsaBI	GATNNNNATC	1 cut(s)	708
BsaI	GGTCTC	1 cut(s)	434
BsaJI	CCNNGG	3 cut(s)	249, 770, 877
BsaWI	WCCGGW	1 cut(s)	899
BsaXI	ACNNNNNCTCC	2 cut(s)	620, 650
Bsc4I	CCNNNNNNNGG	3 cut(s)	764, 883, 1165
Bse118I	RCCGGY	1 cut(s)	950
Bse1I	ACTGG	2 cut(s)	1081, 1102
Bse21I	CCTNAGG	1 cut(s)	1065
Bse3DI	GCAATG	1 cut(s)	1116
Bse8I	GATNNNNATC	1 cut(s)	708
BseBI	CCWGG	5 cut(s)	100, 226, 251, 771, 1059
BseCI	ATCGAT	2 cut(s)	351, 1002
BseDI	CCNNGG	3 cut(s)	249, 770, 877
BseGI	GGATG	6 cut(s)	964, 1020, 1075, 1168, 1177, 1209
BseJI	GATNNNNATC	1 cut(s)	708
BseLI	CCNNNNNNNGG	3 cut(s)	764, 883, 1165
BseMI	GCAATG	1 cut(s)	1116
BseMII	CTCAG	1 cut(s)	720
BseNI	ACTGG	2 cut(s)	1081, 1102
BsePI	GCGCGC	1 cut(s)	887
BseRI	GAGGAG	4 cut(s)	163, 166, 644, 1181
BseXI	GCAGC	3 cut(s)	309, 907, 1306
BseYI	CCCAGC	3 cut(s)	167, 954, 1294
Bsh1236I	CGCG	4 cut(s)	537, 555, 889, 891
Bsh1285I	CGRYCG	1 cut(s)	474
BshFI	GGCC	4 cut(s)	661, 669, 846, 1133
BshVI	ATCGAT	2 cut(s)	351, 1002
BsiEI	CGRYCG	1 cut(s)	474
BsiHKAI	GWGCWC	3 cut(s)	507, 1206, 1245
BsiSI	CCGG	5 cut(s)	395, 508, 900, 951, 1159
BslFI	GGGAC	1 cut(s)	907
BslI	CCNNNNNNNGG	3 cut(s)	764, 883, 1165
BsmAI	GTCTC	2 cut(s)	100, 434
BsmBI	CGTCTC	1 cut(s)	100
BsmFI	GGGAC	1 cut(s)	907
BsmI	GAATGC	1 cut(s)	922
BsnI	GGCC	4 cut(s)	661, 669, 846, 1133
Bso31I	GGTCTC	1 cut(s)	434
Bsp1286I	GDGCHC	4 cut(s)	154, 507, 1206, 1245
Bsp143I	GATC	8 cut(s)	315, 330, 352, 471, 514, 709, 999, 1351
BspACI	CCGC	8 cut(s)	160, 186, 500, 671, 891, 962, 1127, 1298
BspANI	GGCC	4 cut(s)	661, 669, 846, 1133
BspCNI	CTCAG	1 cut(s)	721
BspDI	ATCGAT	2 cut(s)	351, 1002
BspFNI	CGCG	4 cut(s)	537, 555, 889, 891
BspHI	TCATGA	2 cut(s)	700, 829
BspLI	GGNNCC	3 cut(s)	342, 378, 949
BspMI	ACCTGC	3 cut(s)	7, 1016, 1114
BspPI	GGATC	5 cut(s)	323, 347, 509, 1007, 1359
BspTNI	GGTCTC	1 cut(s)	434
BsrDI	GCAATG	1 cut(s)	1116
BsrFI	RCCGGY	1 cut(s)	950
BsrI	ACTGG	2 cut(s)	1081, 1102
BssAI	RCCGGY	1 cut(s)	950
BssECI	CCNNGG	3 cut(s)	249, 770, 877
BssHII	GCGCGC	1 cut(s)	887
BssMI	GATC	8 cut(s)	315, 330, 352, 471, 514, 709, 999, 1351
BssSI	CACGAG	1 cut(s)	1087
Bst2BI	CACGAG	1 cut(s)	1087
Bst2UI	CCWGG	5 cut(s)	100, 226, 251, 771, 1059
Bst4CI	ACNGT	4 cut(s)	46, 125, 212, 1119
Bst6I	CTCTTC	2 cut(s)	105, 1250
BstBAI	YACGTR	1 cut(s)	532
BstC8I	GCNNGC	3 cut(s)	671, 735, 889
BstDEI	CTNAG	5 cut(s)	729, 808, 1065, 1191, 1323
BstDSI	CCRYGG	1 cut(s)	877
BstEII	GGTNACC	1 cut(s)	212
BstF5I	GGATG	6 cut(s)	964, 1020, 1075, 1168, 1177, 1209
BstFNI	CGCG	4 cut(s)	537, 555, 889, 891
BstHHI	GCGC	3 cut(s)	537, 889, 891
BstKTI	GATC	8 cut(s)	318, 333, 355, 474, 517, 712, 1002, 1354
BstMAI	GTCTC	2 cut(s)	100, 434
BstMBI	GATC	8 cut(s)	315, 330, 352, 471, 514, 709, 999, 1351
BstMCI	CGRYCG	1 cut(s)	474
BstMWI	GCNNNNNNNGC	3 cut(s)	168, 421, 888
BstNI	CCWGG	5 cut(s)	100, 226, 251, 771, 1059
BstPI	GGTNACC	1 cut(s)	212
BstSCI	CCNGG	6 cut(s)	98, 224, 249, 769, 1057, 1158
BstSFI	CTRYAG	1 cut(s)	793
BstUI	CGCG	4 cut(s)	537, 555, 889, 891
BstV1I	GCAGC	3 cut(s)	309, 907, 1306
BstV2I	GAAGAC	1 cut(s)	267
Bsu15I	ATCGAT	2 cut(s)	351, 1002
Bsu36I	CCTNAGG	1 cut(s)	1065
BsuRI	GGCC	4 cut(s)	661, 669, 846, 1133
BsuTUI	ATCGAT	2 cut(s)	351, 1002
BtgI	CCRYGG	1 cut(s)	877
BtrI	CACGTC	1 cut(s)	691
BtsCI	GGATG	6 cut(s)	964, 1020, 1075, 1168, 1177, 1209
BveI	ACCTGC	3 cut(s)	7, 1016, 1114
Cac8I	GCNNGC	3 cut(s)	671, 735, 889
CaiI	CAGNNNCTG	1 cut(s)	123
CciI	TCATGA	2 cut(s)	700, 829
CfoI	GCGC	3 cut(s)	537, 889, 891
Cfr10I	RCCGGY	1 cut(s)	950
Cfr13I	GGNCC	6 cut(s)	340, 461, 668, 767, 1055, 1061
ClaI	ATCGAT	2 cut(s)	351, 1002
Csp6I	GTAC	3 cut(s)	823, 860, 1084
CspCI	CAANNNNNGTGG	2 cut(s)	1130, 1165
CviAII	CATG	8 cut(s)	191, 319, 575, 701, 713, 830, 1269, 1336
CviQI	GTAC	3 cut(s)	823, 860, 1084
DdeI	CTNAG	5 cut(s)	729, 808, 1065, 1191, 1323
DpnI	GATC	8 cut(s)	317, 332, 354, 473, 516, 711, 1001, 1353
DpnII	GATC	8 cut(s)	315, 330, 352, 471, 514, 709, 999, 1351
Eam1104I	CTCTTC	2 cut(s)	105, 1250
EarI	CTCTTC	2 cut(s)	105, 1250
Eco147I	AGGCCT	1 cut(s)	661
Eco24I	GRGCYC	1 cut(s)	154
Eco31I	GGTCTC	1 cut(s)	434
Eco47I	GGWCC	5 cut(s)	340, 461, 767, 1055, 1061
Eco57I	CTGAAG	1 cut(s)	773
Eco81I	CCTNAGG	1 cut(s)	1065
Eco91I	GGTNACC	1 cut(s)	212
EcoO109I	RGGNCCY	3 cut(s)	340, 767, 1055
EcoO65I	GGTNACC	1 cut(s)	212
EcoRI	GAATTC	2 cut(s)	24, 568
EcoRII	CCWGG	5 cut(s)	98, 224, 249, 769, 1057
EcoT38I	GRGCYC	1 cut(s)	154
Esp3I	CGTCTC	1 cut(s)	100
FaeI	CATG	8 cut(s)	194, 322, 578, 704, 716, 833, 1272, 1339
FalI	AAGNNNNNCTT	2 cut(s)	1247, 1279
FaqI	GGGAC	1 cut(s)	907
FatI	CATG	8 cut(s)	190, 318, 574, 700, 712, 829, 1268, 1335
FauI	CCCGC	2 cut(s)	678, 884
FauNDI	CATATG	1 cut(s)	1216
FbaI	TGATCA	1 cut(s)	709
FblI	GTMKAC	1 cut(s)	477
Fnu4HI	GCNGC	5 cut(s)	160, 186, 323, 921, 1320
FokI	GGATG	6 cut(s)	971, 1027, 1082, 1175, 1184, 1196
FriOI	GRGCYC	1 cut(s)	154
Fsp4HI	GCNGC	5 cut(s)	160, 186, 323, 921, 1320
FspBI	CTAG	1 cut(s)	762
GlaI	GCGC	3 cut(s)	536, 888, 890
GluI	GCNGC	5 cut(s)	160, 186, 323, 921, 1320
GsaI	CCCAGC	3 cut(s)	171, 958, 1298
HaeIII	GGCC	4 cut(s)	661, 669, 846, 1133
HapII	CCGG	5 cut(s)	395, 508, 900, 951, 1159
HhaI	GCGC	3 cut(s)	537, 889, 891
Hin1II	CATG	8 cut(s)	194, 322, 578, 704, 716, 833, 1272, 1339
Hin6I	GCGC	3 cut(s)	535, 887, 889
HinP1I	GCGC	3 cut(s)	535, 887, 889
HindIII	AAGCTT	1 cut(s)	742
HinfI	GANTC	5 cut(s)	286, 724, 782, 833, 965
HpaII	CCGG	5 cut(s)	395, 508, 900, 951, 1159
HphI	GGTGA	4 cut(s)	224, 616, 629, 1130
Hpy166II	GTNNAC	4 cut(s)	304, 478, 529, 1086
Hpy188I	TCNGA	5 cut(s)	23, 42, 208, 753, 787
Hpy188III	TCNNGA	5 cut(s)	118, 328, 701, 728, 830
Hpy8I	GTNNAC	4 cut(s)	304, 478, 529, 1086
Hpy99I	CGWCG	7 cut(s)	95, 212, 338, 455, 469, 472, 692
HpyAV	CCTTC	3 cut(s)	71, 604, 1051
HpyCH4III	ACNGT	4 cut(s)	46, 125, 212, 1119
HpyCH4IV	ACGT	4 cut(s)	195, 531, 690, 986
HpyCH4V	TGCA	5 cut(s)	144, 424, 931, 1109, 1306
HpyF10VI	GCNNNNNNNGC	3 cut(s)	168, 421, 888
HpyF3I	CTNAG	5 cut(s)	729, 808, 1065, 1191, 1323
HpySE526I	ACGT	4 cut(s)	195, 531, 690, 986
Hsp92II	CATG	8 cut(s)	194, 322, 578, 704, 716, 833, 1272, 1339
HspAI	GCGC	3 cut(s)	535, 887, 889
Ksp22I	TGATCA	1 cut(s)	709
Kzo9I	GATC	8 cut(s)	315, 330, 352, 471, 514, 709, 999, 1351
LmnI	GCTCC	3 cut(s)	92, 176, 510
Lsp1109I	GCAGC	3 cut(s)	309, 907, 1306
LweI	GCATC	6 cut(s)	694, 918, 949, 1027, 1177, 1293
MaeI	CTAG	1 cut(s)	762
MaeII	ACGT	4 cut(s)	195, 531, 690, 986
MaeIII	GTNAC	5 cut(s)	125, 212, 987, 1093, 1339
MalI	GATC	8 cut(s)	317, 332, 354, 473, 516, 711, 1001, 1353
MboI	GATC	8 cut(s)	315, 330, 352, 471, 514, 709, 999, 1351
MboII	GAAGA	7 cut(s)	16, 115, 122, 266, 272, 619, 1267
MfeI	CAATTG	1 cut(s)	596
MhlI	GDGCHC	4 cut(s)	154, 507, 1206, 1245
MluCI	AATT	5 cut(s)	24, 568, 596, 1074, 1153
MlyI	GAGTC	1 cut(s)	974
MmeI	TCCRAC	2 cut(s)	231, 465
MroXI	GAANNNNTTC	1 cut(s)	368
MseI	TTAA	3 cut(s)	804, 866, 1363
MslI	CAYNNNNRTG	2 cut(s)	717, 1248
MspA1I	CMGCKG	1 cut(s)	1298
MspI	CCGG	5 cut(s)	395, 508, 900, 951, 1159
MspR9I	CCNGG	6 cut(s)	100, 226, 251, 771, 1059, 1160
MunI	CAATTG	1 cut(s)	596
Mva1269I	GAATGC	1 cut(s)	922
MvaI	CCWGG	5 cut(s)	100, 226, 251, 771, 1059
MvnI	CGCG	4 cut(s)	537, 555, 889, 891
MwoI	GCNNNNNNNGC	3 cut(s)	168, 421, 888
NciI	CCSGG	1 cut(s)	1160
NdeI	CATATG	1 cut(s)	1216
NdeII	GATC	8 cut(s)	315, 330, 352, 471, 514, 709, 999, 1351
NlaIII	CATG	8 cut(s)	194, 322, 578, 704, 716, 833, 1272, 1339
NlaIV	GGNNCC	3 cut(s)	342, 378, 949
NmeAIII	GCCGAG	1 cut(s)	949
NmuCI	GTSAC	2 cut(s)	212, 987
PagI	TCATGA	2 cut(s)	700, 829
PaqCI	CACCTGC	1 cut(s)	1114
PauI	GCGCGC	1 cut(s)	887
PceI	AGGCCT	1 cut(s)	661
PcsI	WCGNNNNNNNCGW	1 cut(s)	237
PctI	GAATGC	1 cut(s)	922
PdmI	GAANNNNTTC	1 cut(s)	368
PfeI	GAWTC	4 cut(s)	286, 724, 782, 833
PfoI	TCCNGGA	1 cut(s)	98
PkrI	GCNGC	5 cut(s)	161, 187, 324, 922, 1321
Ple19I	CGATCG	1 cut(s)	474
PleI	GAGTC	1 cut(s)	973
PpsI	GAGTC	1 cut(s)	973
Ppu21I	YACGTR	1 cut(s)	532
PpuMI	RGGWCCY	3 cut(s)	340, 767, 1055
Psp5II	RGGWCCY	3 cut(s)	340, 767, 1055
Psp6I	CCWGG	5 cut(s)	98, 224, 249, 769, 1057
PspEI	GGTNACC	1 cut(s)	212
PspFI	CCCAGC	3 cut(s)	167, 954, 1294
PspGI	CCWGG	5 cut(s)	98, 224, 249, 769, 1057
PspN4I	GGNNCC	3 cut(s)	342, 378, 949
PspPI	GGNCC	6 cut(s)	340, 461, 668, 767, 1055, 1061
PspPPI	RGGWCCY	3 cut(s)	340, 767, 1055
PstNI	CAGNNNCTG	1 cut(s)	123
PteI	GCGCGC	1 cut(s)	887
PvuI	CGATCG	1 cut(s)	474
RsaI	GTAC	3 cut(s)	824, 861, 1085
RsaNI	GTAC	3 cut(s)	823, 860, 1084
RseI	CAYNNNNRTG	2 cut(s)	717, 1248
SaqAI	TTAA	3 cut(s)	804, 866, 1363
SatI	GCNGC	5 cut(s)	160, 186, 323, 921, 1320
Sau3AI	GATC	8 cut(s)	315, 330, 352, 471, 514, 709, 999, 1351
Sau96I	GGNCC	6 cut(s)	340, 461, 668, 767, 1055, 1061
SchI	GAGTC	1 cut(s)	974
ScrFI	CCNGG	6 cut(s)	100, 226, 251, 771, 1059, 1160
SduI	GDGCHC	4 cut(s)	154, 507, 1206, 1245
SfaNI	GCATC	6 cut(s)	694, 918, 949, 1027, 1177, 1293
SfcI	CTRYAG	1 cut(s)	793
SinI	GGWCC	5 cut(s)	340, 461, 767, 1055, 1061
SmiMI	CAYNNNNRTG	2 cut(s)	717, 1248
SmlI	CTYRAG	2 cut(s)	275, 326
SmoI	CTYRAG	2 cut(s)	275, 326
Sse9I	AATT	5 cut(s)	24, 568, 596, 1074, 1153
SseBI	AGGCCT	1 cut(s)	661
SsiI	CCGC	8 cut(s)	160, 186, 500, 671, 891, 962, 1127, 1298
SspMI	CTAG	1 cut(s)	762
StuI	AGGCCT	1 cut(s)	661
StyD4I	CCNGG	6 cut(s)	98, 224, 249, 769, 1057, 1158
TaaI	ACNGT	4 cut(s)	46, 125, 212, 1119
TaiI	ACGT	4 cut(s)	198, 534, 693, 989
TaqI	TCGA	8 cut(s)	34, 333, 351, 372, 470, 687, 1002, 1035
TasI	AATT	5 cut(s)	24, 568, 596, 1074, 1153
TatI	WGTACW	2 cut(s)	822, 1083
TauI	GCSGC	2 cut(s)	162, 188
TfiI	GAWTC	4 cut(s)	286, 724, 782, 833
Tru1I	TTAA	3 cut(s)	804, 866, 1363
Tru9I	TTAA	3 cut(s)	804, 866, 1363
TseFI	GTSAC	2 cut(s)	212, 987
TseI	GCWGC	3 cut(s)	322, 920, 1319
Tsp45I	GTSAC	2 cut(s)	212, 987
TspDTI	ATGAA	5 cut(s)	17, 273, 581, 689, 818
TspGWI	ACGGA	2 cut(s)	79, 453
VpaK11BI	GGWCC	5 cut(s)	340, 461, 767, 1055, 1061
XapI	RAATTY	2 cut(s)	24, 568
XmiI	GTMKAC	1 cut(s)	477
XmnI	GAANNNNTTC	1 cut(s)	368
XspI	CTAG	1 cut(s)	762

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.