FvH4_2g04090

lob domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003322
Physical Location & Seq
Reverse (-)
3220601 .. 3222124
1524 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g04090.t1

Sequence Viewer

Length: 642 bp
ATGAGCTGCAATGGATGCCGAGTTTTGCGAAAGGGTTGCAGCGAGAATTGCATGCTACGTCAATGCATAGAGTGGATGGAAGACCCTCAAGCTCAAGCTCACGCCACCGTCTTCGTCGCCAAGTTCTTCGGCCGCGCCGGCCTCCTCTCCTTCATAAACGCCGTCTCCGAAACCCAAAGACCTGCTTTGTTTCAGTCTTTGCTGTTTGAGGCGGTGGGGAGGACTATAAACCCGGTGAACGGAGCGACAGGGCTTCTTTTGGCTGGGAACTGGAAGGTTTGCCAGGCTGCGGTGGAGAGGGTGATGAGCGGCGGCGCGTTGGAGCCACTTCCCGAGTACAGTCGTCGTGGTGTTCATCATGTTAAGCAACAACGGCGGGAAGAGCGCTATTTCTCCAAGTACTCGAACAAAAGGAGACCGGCGTTGTTGGCTTCCGGACACGTCGGTGATGACGAACGGCCTGATCTTGATCTTAGTTTGAAGGCATCGTCGGAGTTGCGTAGACATGATCATGATAGAGCAGCAACGCCGTCGTCTGTGGAAGGCTCGGAAACATCGACGTTCTCAAGAAGTGGTTCGTCGTCGGCAGATTATACAACTAATGATTTAGAGGGAACAAAGCTTCTGAGACTATTCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

214

Amino Acids

23.58

Weight (kDa)

8.8

Isoelectric Point (pI)

55.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 2 - 100 2.7e-23 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 190
AccBSI CCGCTC 1 cut(s) 309
AccI GTMKAC 1 cut(s) 502
AccII CGCG 2 cut(s) 135, 317
AccIII TCCGGA 1 cut(s) 434
AciI CCGC 6 cut(s) 133, 212, 290, 309, 312, 376
AcoI YGGCCR 1 cut(s) 130
AfaI GTAC 2 cut(s) 338, 401
AfeI AGCGCT 1 cut(s) 386
AfiI CCNNNNNNNGG 2 cut(s) 239, 289
AflIII ACRYGT 1 cut(s) 439
AgsI TTSAA 1 cut(s) 481
AjiI CACGTC 1 cut(s) 442
AjnI CCWGG 1 cut(s) 282
AleI CACNNNNGTG 1 cut(s) 444
AluBI AGCT 4 cut(s) 6, 92, 98, 622
AluI AGCT 4 cut(s) 6, 92, 98, 622
Alw26I GTCTC 3 cut(s) 169, 409, 622
Ama87I CYCGRG 1 cut(s) 332
Aor13HI TCCGGA 1 cut(s) 434
Aor51HI AGCGCT 1 cut(s) 386
AoxI GGCC 3 cut(s) 130, 139, 458
ApeKI GCWGC 4 cut(s) 6, 39, 287, 521
ArsI GACNNNNNNTTYG 1 cut(s) 621
Asp700I GAANNNNTTC 1 cut(s) 574
AspLEI GCGC 3 cut(s) 137, 317, 387
AsuC2I CCSGG 1 cut(s) 233
AsuHPI GGTGA 3 cut(s) 247, 313, 458
AvaI CYCGRG 1 cut(s) 332
BbsI GAAGAC 2 cut(s) 87, 103
BbvI GCAGC 3 cut(s) 51, 274, 533
BccI CCATC 1 cut(s) 70
BceAI ACGGC 4 cut(s) 146, 389, 473, 514
BcgI CGANNNNNNTGC 4 cut(s) 18, 52, 513, 547
BciT130I CCWGG 1 cut(s) 284
BclI TGATCA 1 cut(s) 508
BcnI CCSGG 1 cut(s) 233
BcoDI GTCTC 3 cut(s) 169, 409, 622
BfoI RGCGCY 1 cut(s) 388
BfuAI ACCTGC 1 cut(s) 190
BglI GCCNNNNNGGC 1 cut(s) 138
BisI GCNGC 7 cut(s) 7, 40, 133, 288, 310, 313, 522
BlsI GCNGC 7 cut(s) 8, 41, 134, 289, 311, 314, 523
BmcAI AGTACT 1 cut(s) 401
Bme1390I CCNGG 2 cut(s) 233, 284
BmeT110I CYCGRG 1 cut(s) 332
BmgBI CACGTC 1 cut(s) 442
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 2 cut(s) 233, 284
BmsI GCATC 2 cut(s) 5, 494
BpiI GAAGAC 2 cut(s) 87, 103
BpuEI CTTGAG 3 cut(s) 72, 78, 550
BpuMI CCSGG 1 cut(s) 233
BsaBI GATNNNNATC 1 cut(s) 468
BsaI GGTCTC 1 cut(s) 409
BsaWI WCCGGW 1 cut(s) 434
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 2 cut(s) 239, 289
Bse118I RCCGGY 2 cut(s) 137, 418
Bse1I ACTGG 1 cut(s) 275
Bse3DI GCAATG 1 cut(s) 16
Bse8I GATNNNNATC 1 cut(s) 468
BseAI TCCGGA 1 cut(s) 434
BseBI CCWGG 1 cut(s) 284
BseGI GGATG 2 cut(s) 20, 81
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 2 cut(s) 239, 289
BseMI GCAATG 1 cut(s) 16
BseMII CTCAG 1 cut(s) 617
BseNI ACTGG 1 cut(s) 275
BseRI GAGGAG 1 cut(s) 134
BseX3I CGGCCG 1 cut(s) 130
BseXI GCAGC 3 cut(s) 51, 274, 533
BseYI CCCAGC 1 cut(s) 263
Bsh1236I CGCG 2 cut(s) 135, 317
Bsh1285I CGRYCG 1 cut(s) 133
BshFI GGCC 3 cut(s) 132, 141, 460
BsiEI CGRYCG 1 cut(s) 133
BsiHKCI CYCGRG 1 cut(s) 332
BsiSI CCGG 4 cut(s) 138, 233, 419, 435
BslI CCNNNNNNNGG 2 cut(s) 239, 289
BsmAI GTCTC 3 cut(s) 169, 409, 622
BsmBI CGTCTC 1 cut(s) 169
BsnI GGCC 3 cut(s) 132, 141, 460
Bso31I GGTCTC 1 cut(s) 409
BsoBI CYCGRG 1 cut(s) 332
Bsp13I TCCGGA 1 cut(s) 434
Bsp143I GATC 3 cut(s) 463, 469, 508
BspACI CCGC 6 cut(s) 133, 212, 290, 309, 312, 376
BspANI GGCC 3 cut(s) 132, 141, 460
BspCNI CTCAG 1 cut(s) 618
BspEI TCCGGA 1 cut(s) 434
BspFNI CGCG 2 cut(s) 135, 317
BspHI TCATGA 1 cut(s) 511
BspLI GGNNCC 1 cut(s) 324
BspMI ACCTGC 1 cut(s) 190
BspQI GCTCTTC 1 cut(s) 375
BspTNI GGTCTC 1 cut(s) 409
BsrBI CCGCTC 1 cut(s) 309
BsrDI GCAATG 1 cut(s) 16
BsrFI RCCGGY 2 cut(s) 137, 418
BsrI ACTGG 1 cut(s) 275
BssAI RCCGGY 2 cut(s) 137, 418
BssMI GATC 3 cut(s) 463, 469, 508
Bst2UI CCWGG 1 cut(s) 284
Bst4CI ACNGT 2 cut(s) 109, 341
Bst6I CTCTTC 1 cut(s) 375
BstAPI GCANNNNNTGC 1 cut(s) 15
BstC8I GCNNGC 2 cut(s) 53, 139
BstDEI CTNAG 2 cut(s) 473, 626
BstF5I GGATG 2 cut(s) 20, 81
BstFNI CGCG 2 cut(s) 135, 317
BstH2I RGCGCY 1 cut(s) 388
BstHHI GCGC 3 cut(s) 137, 317, 387
BstKTI GATC 3 cut(s) 466, 472, 511
BstMAI GTCTC 3 cut(s) 169, 409, 622
BstMBI GATC 3 cut(s) 463, 469, 508
BstMCI CGRYCG 1 cut(s) 133
BstMWI GCNNNNNNNGC 6 cut(s) 15, 48, 138, 373, 382, 428
BstNI CCWGG 1 cut(s) 284
BstNSI RCATGY 1 cut(s) 55
BstSCI CCNGG 2 cut(s) 231, 282
BstUI CGCG 2 cut(s) 135, 317
BstV1I GCAGC 3 cut(s) 51, 274, 533
BstV2I GAAGAC 2 cut(s) 87, 103
BstZI CGGCCG 1 cut(s) 130
BsuRI GGCC 3 cut(s) 132, 141, 460
BtrI CACGTC 1 cut(s) 442
BtsCI GGATG 2 cut(s) 20, 81
BveI ACCTGC 1 cut(s) 190
Cac8I GCNNGC 2 cut(s) 53, 139
CciI TCATGA 1 cut(s) 511
CfoI GCGC 3 cut(s) 137, 317, 387
Cfr10I RCCGGY 2 cut(s) 137, 418
Csp6I GTAC 2 cut(s) 337, 400
CviAII CATG 4 cut(s) 52, 359, 506, 512
CviQI GTAC 2 cut(s) 337, 400
DdeI CTNAG 2 cut(s) 473, 626
DpnI GATC 3 cut(s) 465, 471, 510
DpnII GATC 3 cut(s) 463, 469, 508
EaeI YGGCCR 1 cut(s) 130
EagI CGGCCG 1 cut(s) 130
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
EclXI CGGCCG 1 cut(s) 130
Eco31I GGTCTC 1 cut(s) 409
Eco47III AGCGCT 1 cut(s) 386
Eco52I CGGCCG 1 cut(s) 130
Eco88I CYCGRG 1 cut(s) 332
EcoRII CCWGG 1 cut(s) 282
EcoT22I ATGCAT 1 cut(s) 68
Esp3I CGTCTC 1 cut(s) 169
FaeI CATG 4 cut(s) 55, 362, 509, 515
FaiI YATR 8 cut(s) 53, 68, 155, 227, 360, 507, 513, 594
FalI AAGNNNNNCTT 2 cut(s) 169, 201
FatI CATG 4 cut(s) 51, 358, 505, 511
FauI CCCGC 1 cut(s) 369
FbaI TGATCA 1 cut(s) 508
FblI GTMKAC 1 cut(s) 502
Fnu4HI GCNGC 7 cut(s) 7, 40, 133, 288, 310, 313, 522
FokI GGATG 2 cut(s) 27, 88
Fsp4HI GCNGC 7 cut(s) 7, 40, 133, 288, 310, 313, 522
GlaI GCGC 3 cut(s) 136, 316, 386
GluI GCNGC 7 cut(s) 7, 40, 133, 288, 310, 313, 522
GsaI CCCAGC 1 cut(s) 267
HaeII RGCGCY 1 cut(s) 388
HaeIII GGCC 3 cut(s) 132, 141, 460
HapII CCGG 4 cut(s) 138, 233, 419, 435
HhaI GCGC 3 cut(s) 137, 317, 387
Hin1II CATG 4 cut(s) 55, 362, 509, 515
Hin6I GCGC 3 cut(s) 135, 315, 385
HinP1I GCGC 3 cut(s) 135, 315, 385
HindIII AAGCTT 1 cut(s) 620
HpaII CCGG 4 cut(s) 138, 233, 419, 435
HphI GGTGA 3 cut(s) 247, 313, 458
Hpy166II GTNNAC 2 cut(s) 238, 503
Hpy188I TCNGA 4 cut(s) 169, 493, 550, 627
Hpy188III TCNNGA 5 cut(s) 332, 435, 467, 512, 567
Hpy8I GTNNAC 2 cut(s) 238, 503
Hpy99I CGWCG 8 cut(s) 119, 348, 446, 493, 535, 562, 583, 586
HpyAV CCTTC 4 cut(s) 160, 268, 475, 536
HpyCH4III ACNGT 2 cut(s) 109, 341
HpyCH4IV ACGT 3 cut(s) 58, 441, 560
HpyCH4V TGCA 4 cut(s) 9, 39, 51, 66
HpyF10VI GCNNNNNNNGC 6 cut(s) 15, 48, 138, 373, 382, 428
HpyF3I CTNAG 2 cut(s) 473, 626
HpySE526I ACGT 3 cut(s) 58, 441, 560
Hsp92II CATG 4 cut(s) 55, 362, 509, 515
HspAI GCGC 3 cut(s) 135, 315, 385
Kpn2I TCCGGA 1 cut(s) 434
KroI GCCGGC 1 cut(s) 137
KroNI GCCGGC 1 cut(s) 139
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 3 cut(s) 463, 469, 508
LguI GCTCTTC 1 cut(s) 375
LmnI GCTCC 2 cut(s) 242, 322
Lsp1109I GCAGC 3 cut(s) 51, 274, 533
LweI GCATC 2 cut(s) 5, 494
MaeII ACGT 3 cut(s) 58, 441, 560
MalI GATC 3 cut(s) 465, 471, 510
MbiI CCGCTC 1 cut(s) 309
MboI GATC 3 cut(s) 463, 469, 508
MboII GAAGA 4 cut(s) 92, 103, 118, 392
MluCI AATT 1 cut(s) 46
MmeI TCCRAC 2 cut(s) 300, 471
MnlI CCTC 7 cut(s) 96, 152, 155, 202, 213, 291, 604
Mph1103I ATGCAT 1 cut(s) 68
MroI TCCGGA 1 cut(s) 434
MroNI GCCGGC 1 cut(s) 137
MroXI GAANNNNTTC 1 cut(s) 574
MseI TTAA 1 cut(s) 363
MslI CAYNNNNRTG 2 cut(s) 444, 510
MspI CCGG 4 cut(s) 138, 233, 419, 435
MspR9I CCNGG 2 cut(s) 233, 284
MvaI CCWGG 1 cut(s) 284
MvnI CGCG 2 cut(s) 135, 317
MwoI GCNNNNNNNGC 6 cut(s) 15, 48, 138, 373, 382, 428
NaeI GCCGGC 1 cut(s) 139
NciI CCSGG 1 cut(s) 233
NdeII GATC 3 cut(s) 463, 469, 508
NgoMIV GCCGGC 1 cut(s) 137
NlaIII CATG 4 cut(s) 55, 362, 509, 515
NlaIV GGNNCC 1 cut(s) 324
NmeAIII GCCGAG 1 cut(s) 44
NsiI ATGCAT 1 cut(s) 68
NspI RCATGY 1 cut(s) 55
OliI CACNNNNGTG 1 cut(s) 444
PaeI GCATGC 1 cut(s) 55
PagI TCATGA 1 cut(s) 511
PciSI GCTCTTC 1 cut(s) 375
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 450, 554
PdiI GCCGGC 1 cut(s) 139
PdmI GAANNNNTTC 1 cut(s) 574
PkrI GCNGC 7 cut(s) 8, 41, 134, 289, 311, 314, 523
Psp6I CCWGG 1 cut(s) 282
PspFI CCCAGC 1 cut(s) 263
PspGI CCWGG 1 cut(s) 282
PspN4I GGNNCC 1 cut(s) 324
RsaI GTAC 2 cut(s) 338, 401
RsaNI GTAC 2 cut(s) 337, 400
RseI CAYNNNNRTG 2 cut(s) 444, 510
SapI GCTCTTC 1 cut(s) 375
SaqAI TTAA 1 cut(s) 363
SatI GCNGC 7 cut(s) 7, 40, 133, 288, 310, 313, 522
Sau3AI GATC 3 cut(s) 463, 469, 508
ScaI AGTACT 1 cut(s) 401
ScrFI CCNGG 2 cut(s) 233, 284
SetI ASST 9 cut(s) 8, 61, 94, 100, 184, 279, 444, 563, 624
SfaNI GCATC 2 cut(s) 5, 494
SfiI GGCCNNNNNGGCC 1 cut(s) 138
SmiMI CAYNNNNRTG 2 cut(s) 444, 510
SmlI CTYRAG 3 cut(s) 87, 93, 565
SmoI CTYRAG 3 cut(s) 87, 93, 565
SphI GCATGC 1 cut(s) 55
Sse9I AATT 1 cut(s) 46
SsiI CCGC 6 cut(s) 133, 212, 290, 309, 312, 376
StyD4I CCNGG 2 cut(s) 231, 282
TaaI ACNGT 2 cut(s) 109, 341
TaiI ACGT 3 cut(s) 61, 444, 563
TaqI TCGA 2 cut(s) 404, 557
TasI AATT 1 cut(s) 46
TatI WGTACW 2 cut(s) 336, 399
TauI GCSGC 3 cut(s) 135, 312, 315
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TseI GCWGC 4 cut(s) 6, 39, 287, 521
TspDTI ATGAA 2 cut(s) 142, 344
TspGWI ACGGA 1 cut(s) 255
XceI RCATGY 1 cut(s) 55
XmiI GTMKAC 1 cut(s) 502
XmnI GAANNNNTTC 1 cut(s) 574
ZrmI AGTACT 1 cut(s) 401
Zsp2I ATGCAT 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.