RchiOBHm_Chr6g0253441

lob domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003322
Physical Location & Seq
Reverse (-)
8539580 .. 8540303
724 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22726

Sequence Viewer

Length: 600 bp
ATGAGCTGCAATGGATGCCGAGTTTTGCGAAAGGGTTGCAGCGAGAATTGCATGCTACGTCAATGCATAGAGTGGATGGAAGACCCTCAAGCTCAAGCTCACGCCACCGTTTTCGTCGCCAAGTTTTTCGGCCGCGCCGGCCTCCTCTCCTTCATCAACGCTGTCTCCGAAAGCCAAAGACCAGGGAGGACTATAAATCCGGTGAACGGAGCGACGGGACTTCTTTTGGCTGGGAACTGGAAGGTCTGCCAGGCTGCGGTGGAGAAGGTGCTGAGCGGCGGCGCGTTGGAGCCACTTCCCGAGTACAGTTGTCGTGGCATTCATCACGTTCGACAACAACGGCGGGATGAGCGCTATTCCTCCAAGTACTCGACCAAAAAGAGGCCGGCTCCATTGGCTTTCGACGATGATGAACGGCCTGATCTTGATCTTAGCTTGAAGGCATCGTCGGAGTTGCATAGAGATGATCACGATAGAGCGGCGACGCCGTCGTCTGTGGAAGGCTCAGAAACGACGATGTTGTCAAGAAGTGGTTCGTCGTCGGCAGATTGTACTACTAATGATTCAGAGGGAACAAGGCTTCTAAGACTATTCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

199

Amino Acids

21.89

Weight (kDa)

7.59

Isoelectric Point (pI)

50.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 2 - 88 1.1e-12 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 490, 520
AccBSI CCGCTC 2 cut(s) 276, 479
AccII CGCG 2 cut(s) 135, 284
AciI CCGC 6 cut(s) 133, 257, 276, 279, 343, 479
AcoI YGGCCR 1 cut(s) 130
AcyI GRCGYC 1 cut(s) 485
AfaI GTAC 3 cut(s) 305, 368, 553
AfeI AGCGCT 1 cut(s) 353
AfiI CCNNNNNNNGG 3 cut(s) 206, 256, 381
AgsI TTSAA 1 cut(s) 439
AjnI CCWGG 2 cut(s) 181, 249
AluBI AGCT 4 cut(s) 6, 92, 98, 435
AluI AGCT 4 cut(s) 6, 92, 98, 435
Alw26I GTCTC 1 cut(s) 169
Ama87I CYCGRG 1 cut(s) 299
Aor51HI AGCGCT 1 cut(s) 353
AoxI GGCC 4 cut(s) 130, 139, 383, 416
ApeKI GCWGC 3 cut(s) 6, 39, 254
ArsI GACNNNNNNTTYG 1 cut(s) 579
Asp700I GAANNNNTTC 1 cut(s) 532
AspLEI GCGC 3 cut(s) 137, 284, 354
AsuHPI GGTGA 1 cut(s) 214
AvaI CYCGRG 1 cut(s) 299
BbsI GAAGAC 1 cut(s) 87
BbvI GCAGC 2 cut(s) 51, 241
BccI CCATC 1 cut(s) 70
BceAI ACGGC 3 cut(s) 356, 431, 472
BcgI CGANNNNNNTGC 2 cut(s) 18, 52
BciT130I CCWGG 2 cut(s) 183, 251
BclI TGATCA 1 cut(s) 466
BcoDI GTCTC 1 cut(s) 169
BfoI RGCGCY 1 cut(s) 355
BglI GCCNNNNNGGC 1 cut(s) 138
BisI GCNGC 7 cut(s) 7, 40, 133, 255, 277, 280, 480
BlpI GCTNAGC 1 cut(s) 272
BlsI GCNGC 7 cut(s) 8, 41, 134, 256, 278, 281, 481
BmcAI AGTACT 1 cut(s) 368
Bme1390I CCNGG 2 cut(s) 183, 251
BmeT110I CYCGRG 1 cut(s) 299
BmiI GGNNCC 2 cut(s) 291, 390
BmrFI CCNGG 2 cut(s) 183, 251
BmsI GCATC 2 cut(s) 5, 452
BpiI GAAGAC 1 cut(s) 87
BplI GAGNNNNNCTC 2 cut(s) 373, 405
Bpu1102I GCTNAGC 1 cut(s) 272
BpuEI CTTGAG 2 cut(s) 72, 78
BsaBI GATNNNNATC 1 cut(s) 426
BsaHI GRCGYC 1 cut(s) 485
BsaJI CCNNGG 1 cut(s) 182
BsaWI WCCGGW 1 cut(s) 199
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 3 cut(s) 206, 256, 381
Bse118I RCCGGY 2 cut(s) 137, 385
Bse1I ACTGG 1 cut(s) 242
Bse3DI GCAATG 1 cut(s) 16
Bse8I GATNNNNATC 1 cut(s) 426
BseBI CCWGG 2 cut(s) 183, 251
BseDI CCNNGG 1 cut(s) 182
BseGI GGATG 3 cut(s) 20, 81, 352
BseJI GATNNNNATC 1 cut(s) 426
BseLI CCNNNNNNNGG 3 cut(s) 206, 256, 381
BseMI GCAATG 1 cut(s) 16
BseMII CTCAG 2 cut(s) 263, 519
BseNI ACTGG 1 cut(s) 242
BseRI GAGGAG 1 cut(s) 134
BseX3I CGGCCG 1 cut(s) 130
BseXI GCAGC 2 cut(s) 51, 241
BseYI CCCAGC 1 cut(s) 230
Bsh1236I CGCG 2 cut(s) 135, 284
Bsh1285I CGRYCG 1 cut(s) 133
BshFI GGCC 4 cut(s) 132, 141, 385, 418
BsiEI CGRYCG 1 cut(s) 133
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 3 cut(s) 138, 200, 386
BslFI GGGAC 1 cut(s) 231
BslI CCNNNNNNNGG 3 cut(s) 206, 256, 381
BsmAI GTCTC 1 cut(s) 169
BsmFI GGGAC 1 cut(s) 231
BsmI GAATGC 1 cut(s) 318
BsnI GGCC 4 cut(s) 132, 141, 385, 418
BsoBI CYCGRG 1 cut(s) 299
Bsp143I GATC 3 cut(s) 421, 427, 466
Bsp1720I GCTNAGC 1 cut(s) 272
BspACI CCGC 6 cut(s) 133, 257, 276, 279, 343, 479
BspANI GGCC 4 cut(s) 132, 141, 385, 418
BspCNI CTCAG 2 cut(s) 264, 518
BspFNI CGCG 2 cut(s) 135, 284
BspLI GGNNCC 2 cut(s) 291, 390
BsrBI CCGCTC 2 cut(s) 276, 479
BsrDI GCAATG 1 cut(s) 16
BsrFI RCCGGY 2 cut(s) 137, 385
BsrI ACTGG 1 cut(s) 242
BssAI RCCGGY 2 cut(s) 137, 385
BssECI CCNNGG 1 cut(s) 182
BssMI GATC 3 cut(s) 421, 427, 466
BssNI GRCGYC 1 cut(s) 485
Bst2UI CCWGG 2 cut(s) 183, 251
Bst4CI ACNGT 2 cut(s) 109, 308
BstACI GRCGYC 1 cut(s) 485
BstAPI GCANNNNNTGC 1 cut(s) 15
BstC8I GCNNGC 3 cut(s) 53, 139, 387
BstDEI CTNAG 4 cut(s) 272, 431, 505, 584
BstF5I GGATG 3 cut(s) 20, 81, 352
BstFNI CGCG 2 cut(s) 135, 284
BstH2I RGCGCY 1 cut(s) 355
BstHHI GCGC 3 cut(s) 137, 284, 354
BstKTI GATC 3 cut(s) 424, 430, 469
BstMAI GTCTC 1 cut(s) 169
BstMBI GATC 3 cut(s) 421, 427, 466
BstMCI CGRYCG 1 cut(s) 133
BstMWI GCNNNNNNNGC 5 cut(s) 15, 48, 138, 349, 395
BstNI CCWGG 2 cut(s) 183, 251
BstNSI RCATGY 1 cut(s) 55
BstSCI CCNGG 2 cut(s) 181, 249
BstUI CGCG 2 cut(s) 135, 284
BstV1I GCAGC 2 cut(s) 51, 241
BstV2I GAAGAC 1 cut(s) 87
BstZI CGGCCG 1 cut(s) 130
BsuRI GGCC 4 cut(s) 132, 141, 385, 418
BtsCI GGATG 3 cut(s) 20, 81, 352
Cac8I GCNNGC 3 cut(s) 53, 139, 387
CfoI GCGC 3 cut(s) 137, 284, 354
Cfr10I RCCGGY 2 cut(s) 137, 385
CseI GACGC 1 cut(s) 493
Csp6I GTAC 3 cut(s) 304, 367, 552
CviAII CATG 1 cut(s) 52
CviQI GTAC 3 cut(s) 304, 367, 552
DdeI CTNAG 4 cut(s) 272, 431, 505, 584
DpnI GATC 3 cut(s) 423, 429, 468
DpnII GATC 3 cut(s) 421, 427, 466
DrdI GACNNNNNNGTC 2 cut(s) 490, 520
DseDI GACNNNNNNGTC 2 cut(s) 490, 520
EaeI YGGCCR 1 cut(s) 130
EagI CGGCCG 1 cut(s) 130
EclXI CGGCCG 1 cut(s) 130
Eco47III AGCGCT 1 cut(s) 353
Eco52I CGGCCG 1 cut(s) 130
Eco88I CYCGRG 1 cut(s) 299
EcoRII CCWGG 2 cut(s) 181, 249
EcoT22I ATGCAT 1 cut(s) 68
FaeI CATG 1 cut(s) 55
FaiI YATR 4 cut(s) 53, 68, 194, 459
FaqI GGGAC 1 cut(s) 231
FatI CATG 1 cut(s) 51
FauI CCCGC 1 cut(s) 336
FbaI TGATCA 1 cut(s) 466
Fnu4HI GCNGC 7 cut(s) 7, 40, 133, 255, 277, 280, 480
FokI GGATG 3 cut(s) 27, 88, 359
Fsp4HI GCNGC 7 cut(s) 7, 40, 133, 255, 277, 280, 480
GlaI GCGC 3 cut(s) 136, 283, 353
GluI GCNGC 7 cut(s) 7, 40, 133, 255, 277, 280, 480
GsaI CCCAGC 1 cut(s) 234
HaeII RGCGCY 1 cut(s) 355
HaeIII GGCC 4 cut(s) 132, 141, 385, 418
HapII CCGG 3 cut(s) 138, 200, 386
HgaI GACGC 1 cut(s) 493
HhaI GCGC 3 cut(s) 137, 284, 354
Hin1I GRCGYC 1 cut(s) 485
Hin1II CATG 1 cut(s) 55
Hin6I GCGC 3 cut(s) 135, 282, 352
HinP1I GCGC 3 cut(s) 135, 282, 352
HinfI GANTC 1 cut(s) 563
HpaII CCGG 3 cut(s) 138, 200, 386
HphI GGTGA 1 cut(s) 214
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 4 cut(s) 169, 451, 508, 568
Hpy188III TCNNGA 4 cut(s) 299, 425, 470, 525
Hpy8I GTNNAC 1 cut(s) 205
Hpy99I CGWCG 9 cut(s) 119, 217, 407, 451, 487, 493, 517, 541, 544
HpyAV CCTTC 5 cut(s) 160, 235, 259, 433, 494
HpyCH4III ACNGT 2 cut(s) 109, 308
HpyCH4IV ACGT 2 cut(s) 58, 327
HpyCH4V TGCA 5 cut(s) 9, 39, 51, 66, 457
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 48, 138, 349, 395
HpyF3I CTNAG 4 cut(s) 272, 431, 505, 584
HpySE526I ACGT 2 cut(s) 58, 327
Hsp92I GRCGYC 1 cut(s) 485
Hsp92II CATG 1 cut(s) 55
HspAI GCGC 3 cut(s) 135, 282, 352
KroI GCCGGC 2 cut(s) 137, 385
KroNI GCCGGC 2 cut(s) 139, 387
Ksp22I TGATCA 1 cut(s) 466
Kzo9I GATC 3 cut(s) 421, 427, 466
LmnI GCTCC 3 cut(s) 209, 289, 394
Lsp1109I GCAGC 2 cut(s) 51, 241
LweI GCATC 2 cut(s) 5, 452
MaeII ACGT 2 cut(s) 58, 327
MalI GATC 3 cut(s) 423, 429, 468
MbiI CCGCTC 2 cut(s) 276, 479
MboI GATC 3 cut(s) 421, 427, 466
MboII GAAGA 1 cut(s) 92
MluCI AATT 1 cut(s) 46
MmeI TCCRAC 2 cut(s) 267, 429
MnlI CCTC 7 cut(s) 96, 152, 155, 180, 370, 375, 562
Mph1103I ATGCAT 1 cut(s) 68
MroNI GCCGGC 2 cut(s) 137, 385
MroXI GAANNNNTTC 1 cut(s) 532
MslI CAYNNNNRTG 1 cut(s) 462
MspI CCGG 3 cut(s) 138, 200, 386
MspR9I CCNGG 2 cut(s) 183, 251
Mva1269I GAATGC 1 cut(s) 318
MvaI CCWGG 2 cut(s) 183, 251
MvnI CGCG 2 cut(s) 135, 284
MwoI GCNNNNNNNGC 5 cut(s) 15, 48, 138, 349, 395
NaeI GCCGGC 2 cut(s) 139, 387
NdeII GATC 3 cut(s) 421, 427, 466
NgoMIV GCCGGC 2 cut(s) 137, 385
NlaIII CATG 1 cut(s) 55
NlaIV GGNNCC 2 cut(s) 291, 390
NmeAIII GCCGAG 1 cut(s) 44
NsiI ATGCAT 1 cut(s) 68
NspI RCATGY 1 cut(s) 55
PaeI GCATGC 1 cut(s) 55
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PctI GAATGC 1 cut(s) 318
PdiI GCCGGC 2 cut(s) 139, 387
PdmI GAANNNNTTC 1 cut(s) 532
PfeI GAWTC 1 cut(s) 563
PflFI GACNNNGTC 1 cut(s) 487
PkrI GCNGC 7 cut(s) 8, 41, 134, 256, 278, 281, 481
Psp6I CCWGG 2 cut(s) 181, 249
PspFI CCCAGC 1 cut(s) 230
PspGI CCWGG 2 cut(s) 181, 249
PspN4I GGNNCC 2 cut(s) 291, 390
PsyI GACNNNGTC 1 cut(s) 487
RsaI GTAC 3 cut(s) 305, 368, 553
RsaNI GTAC 3 cut(s) 304, 367, 552
RseI CAYNNNNRTG 1 cut(s) 462
SatI GCNGC 7 cut(s) 7, 40, 133, 255, 277, 280, 480
Sau3AI GATC 3 cut(s) 421, 427, 466
ScaI AGTACT 1 cut(s) 368
ScrFI CCNGG 2 cut(s) 183, 251
SetI ASST 8 cut(s) 8, 61, 94, 100, 246, 270, 330, 437
SfaNI GCATC 2 cut(s) 5, 452
SfiI GGCCNNNNNGGCC 1 cut(s) 138
SmiMI CAYNNNNRTG 1 cut(s) 462
SmlI CTYRAG 2 cut(s) 87, 93
SmoI CTYRAG 2 cut(s) 87, 93
SphI GCATGC 1 cut(s) 55
Sse9I AATT 1 cut(s) 46
SsiI CCGC 6 cut(s) 133, 257, 276, 279, 343, 479
StyD4I CCNGG 2 cut(s) 181, 249
TaaI ACNGT 2 cut(s) 109, 308
TaiI ACGT 2 cut(s) 61, 330
TaqI TCGA 3 cut(s) 331, 371, 402
TasI AATT 1 cut(s) 46
TatI WGTACW 3 cut(s) 303, 366, 551
TauI GCSGC 4 cut(s) 135, 279, 282, 482
TfiI GAWTC 1 cut(s) 563
TseI GCWGC 3 cut(s) 6, 39, 254
TspDTI ATGAA 3 cut(s) 142, 311, 426
TspGWI ACGGA 1 cut(s) 222
Tth111I GACNNNGTC 1 cut(s) 487
XceI RCATGY 1 cut(s) 55
XmnI GAANNNNTTC 1 cut(s) 532
ZrmI AGTACT 1 cut(s) 368
Zsp2I ATGCAT 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.