MD02G1165000.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003322
Physical Location & Seq
Forward (+)
14091705 .. 14092127
423 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1165000.v1.1.491

Sequence Viewer

Length: 327 bp
ATGCAGCAAGACATGCATACTGAGGTCGTGCCTGTATTGGATCGGATGGTGGAGATGAGCTGCAACGGCTGCCGAGTGCTCCGGAAGGGATGCAGCGACACATGCATACTGAGGTCGTGTCTGTACTGGATCGACTCTCCCGAAGCCCAAGGCAACGCCACCTTGTTCCTCGCCAAGTTCTTTAGCCACAATGATCTCCTCTCATTCGTCTTCGTCGTACCGGAAAACCAACGCCCCGCTATGTTTCAGTCTCTGCTGTTCGAAGCGTGCGGCCGCATGGTGAACCCGGTGAACGGAGCGGTGGGGCTTCTGTCGAGTGGGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

109

Amino Acids

12.0

Weight (kDa)

5.53

Isoelectric Point (pI)

61.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 20 - 106 9.8e-20 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 299
AccIII TCCGGA 1 cut(s) 81
AciI CCGC 4 cut(s) 237, 270, 274, 299
AclWI GGATC 2 cut(s) 48, 137
AcoI YGGCCR 1 cut(s) 271
AfaI GTAC 2 cut(s) 125, 219
AfiI CCNNNNNNNGG 1 cut(s) 293
AluBI AGCT 1 cut(s) 60
AluI AGCT 1 cut(s) 60
Alw21I GWGCWC 1 cut(s) 81
Alw26I GTCTC 1 cut(s) 255
AlwI GGATC 2 cut(s) 48, 137
AlwNI CAGNNNCTG 1 cut(s) 253
Aor13HI TCCGGA 1 cut(s) 81
AoxI GGCC 1 cut(s) 271
ApeKI GCWGC 4 cut(s) 4, 60, 69, 93
AsuC2I CCSGG 1 cut(s) 287
AsuHPI GGTGA 2 cut(s) 292, 301
AsuII TTCGAA 1 cut(s) 261
BbsI GAAGAC 1 cut(s) 202
Bbv12I GWGCWC 1 cut(s) 81
BbvI GCAGC 4 cut(s) 16, 47, 56, 105
BccI CCATC 1 cut(s) 40
BceAI ACGGC 1 cut(s) 82
BcnI CCSGG 1 cut(s) 287
BcoDI GTCTC 1 cut(s) 255
BfaI CTAG 1 cut(s) 325
BisI GCNGC 6 cut(s) 5, 61, 70, 94, 271, 274
BlsI GCNGC 6 cut(s) 6, 62, 71, 95, 272, 275
Bme1390I CCNGG 1 cut(s) 287
BmrFI CCNGG 1 cut(s) 287
BmsI GCATC 1 cut(s) 80
BpiI GAAGAC 1 cut(s) 202
Bpu14I TTCGAA 1 cut(s) 261
BpuMI CCSGG 1 cut(s) 287
BsaJI CCNNGG 1 cut(s) 148
BsaWI WCCGGW 2 cut(s) 81, 220
Bsc4I CCNNNNNNNGG 1 cut(s) 293
Bse1I ACTGG 1 cut(s) 131
BseAI TCCGGA 1 cut(s) 81
BseDI CCNNGG 1 cut(s) 148
BseGI GGATG 2 cut(s) 51, 95
BseLI CCNNNNNNNGG 1 cut(s) 293
BseMII CTCAG 2 cut(s) 12, 101
BseNI ACTGG 1 cut(s) 131
BseRI GAGGAG 1 cut(s) 188
BseX3I CGGCCG 1 cut(s) 271
BseXI GCAGC 4 cut(s) 16, 47, 56, 105
Bsh1285I CGRYCG 1 cut(s) 274
BshFI GGCC 1 cut(s) 273
BsiEI CGRYCG 1 cut(s) 274
BsiHKAI GWGCWC 1 cut(s) 81
BsiSI CCGG 3 cut(s) 82, 221, 287
BslI CCNNNNNNNGG 1 cut(s) 293
BsmAI GTCTC 1 cut(s) 255
BsnI GGCC 1 cut(s) 273
Bsp119I TTCGAA 1 cut(s) 261
Bsp1286I GDGCHC 1 cut(s) 81
Bsp13I TCCGGA 1 cut(s) 81
Bsp143I GATC 3 cut(s) 40, 129, 193
BspACI CCGC 4 cut(s) 237, 270, 274, 299
BspANI GGCC 1 cut(s) 273
BspCNI CTCAG 2 cut(s) 13, 102
BspEI TCCGGA 1 cut(s) 81
BspPI GGATC 2 cut(s) 48, 137
BspT104I TTCGAA 1 cut(s) 261
BsrBI CCGCTC 1 cut(s) 299
BsrI ACTGG 1 cut(s) 131
BssECI CCNNGG 1 cut(s) 148
BssMI GATC 3 cut(s) 40, 129, 193
BssT1I CCWWGG 1 cut(s) 148
BstAPI GCANNNNNTGC 2 cut(s) 13, 69
BstBI TTCGAA 1 cut(s) 261
BstC8I GCNNGC 1 cut(s) 268
BstDEI CTNAG 2 cut(s) 21, 110
BstF5I GGATG 2 cut(s) 51, 95
BstKTI GATC 3 cut(s) 43, 132, 196
BstMAI GTCTC 1 cut(s) 255
BstMBI GATC 3 cut(s) 40, 129, 193
BstMCI CGRYCG 1 cut(s) 274
BstMWI GCNNNNNNNGC 4 cut(s) 13, 66, 69, 102
BstNSI RCATGY 2 cut(s) 16, 105
BstSCI CCNGG 1 cut(s) 285
BstV1I GCAGC 4 cut(s) 16, 47, 56, 105
BstV2I GAAGAC 1 cut(s) 202
BstZI CGGCCG 1 cut(s) 271
BsuRI GGCC 1 cut(s) 273
BtsCI GGATG 2 cut(s) 51, 95
Cac8I GCNNGC 1 cut(s) 268
CaiI CAGNNNCTG 1 cut(s) 253
CciNI GCGGCCGC 1 cut(s) 271
Csp6I GTAC 2 cut(s) 124, 218
CviAII CATG 3 cut(s) 13, 102, 277
CviJI RGCY 6 cut(s) 60, 69, 146, 186, 273, 307
CviKI_1 RGCY 6 cut(s) 60, 69, 146, 186, 273, 307
CviQI GTAC 2 cut(s) 124, 218
DdeI CTNAG 2 cut(s) 21, 110
DpnI GATC 3 cut(s) 42, 131, 195
DpnII GATC 3 cut(s) 40, 129, 193
EaeI YGGCCR 1 cut(s) 271
EagI CGGCCG 1 cut(s) 271
EclXI CGGCCG 1 cut(s) 271
Eco130I CCWWGG 1 cut(s) 148
Eco52I CGGCCG 1 cut(s) 271
EcoT14I CCWWGG 1 cut(s) 148
EcoT22I ATGCAT 2 cut(s) 18, 107
ErhI CCWWGG 1 cut(s) 148
FaeI CATG 3 cut(s) 16, 105, 280
FaiI YATR 6 cut(s) 14, 18, 103, 107, 242, 278
FatI CATG 3 cut(s) 12, 101, 276
FauI CCCGC 1 cut(s) 244
Fnu4HI GCNGC 6 cut(s) 5, 61, 70, 94, 271, 274
FokI GGATG 2 cut(s) 58, 102
Fsp4HI GCNGC 6 cut(s) 5, 61, 70, 94, 271, 274
FspBI CTAG 1 cut(s) 325
GluI GCNGC 6 cut(s) 5, 61, 70, 94, 271, 274
HaeIII GGCC 1 cut(s) 273
HapII CCGG 3 cut(s) 82, 221, 287
Hin1II CATG 3 cut(s) 16, 105, 280
HinfI GANTC 1 cut(s) 134
HpaII CCGG 3 cut(s) 82, 221, 287
HphI GGTGA 2 cut(s) 292, 301
Hpy166II GTNNAC 2 cut(s) 283, 292
Hpy188I TCNGA 1 cut(s) 45
Hpy188III TCNNGA 2 cut(s) 82, 140
Hpy8I GTNNAC 2 cut(s) 283, 292
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 1 cut(s) 79
HpyCH4V TGCA 5 cut(s) 4, 16, 63, 93, 105
HpyF10VI GCNNNNNNNGC 4 cut(s) 13, 66, 69, 102
HpyF3I CTNAG 2 cut(s) 21, 110
Hsp92II CATG 3 cut(s) 16, 105, 280
Kpn2I TCCGGA 1 cut(s) 81
Kzo9I GATC 3 cut(s) 40, 129, 193
LmnI GCTCC 2 cut(s) 84, 296
LpnPI CCDG 5 cut(s) 45, 95, 112, 234, 300
Lsp1109I GCAGC 4 cut(s) 16, 47, 56, 105
LweI GCATC 1 cut(s) 80
MaeI CTAG 1 cut(s) 325
MalI GATC 3 cut(s) 42, 131, 195
MbiI CCGCTC 1 cut(s) 299
MboI GATC 3 cut(s) 40, 129, 193
MboII GAAGA 1 cut(s) 202
MhlI GDGCHC 1 cut(s) 81
MlyI GAGTC 1 cut(s) 128
MnlI CCTC 4 cut(s) 16, 105, 179, 209
Mph1103I ATGCAT 2 cut(s) 18, 107
MroI TCCGGA 1 cut(s) 81
MspI CCGG 3 cut(s) 82, 221, 287
MspR9I CCNGG 1 cut(s) 287
MwoI GCNNNNNNNGC 4 cut(s) 13, 66, 69, 102
NciI CCSGG 1 cut(s) 287
NdeII GATC 3 cut(s) 40, 129, 193
NlaIII CATG 3 cut(s) 16, 105, 280
NmeAIII GCCGAG 1 cut(s) 98
NotI GCGGCCGC 1 cut(s) 271
NsiI ATGCAT 2 cut(s) 18, 107
NspI RCATGY 2 cut(s) 16, 105
NspV TTCGAA 1 cut(s) 261
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 213
PkrI GCNGC 6 cut(s) 6, 62, 71, 95, 272, 275
PleI GAGTC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 128
PstNI CAGNNNCTG 1 cut(s) 253
RsaI GTAC 2 cut(s) 125, 219
RsaNI GTAC 2 cut(s) 124, 218
SatI GCNGC 6 cut(s) 5, 61, 70, 94, 271, 274
Sau3AI GATC 3 cut(s) 40, 129, 193
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 1 cut(s) 287
SduI GDGCHC 1 cut(s) 81
SetI ASST 4 cut(s) 27, 62, 116, 164
SfaNI GCATC 1 cut(s) 80
SfuI TTCGAA 1 cut(s) 261
SsiI CCGC 4 cut(s) 237, 270, 274, 299
SspMI CTAG 1 cut(s) 325
StyD4I CCNGG 1 cut(s) 285
StyI CCWWGG 1 cut(s) 148
TaqI TCGA 3 cut(s) 132, 261, 314
TatI WGTACW 1 cut(s) 123
TauI GCSGC 2 cut(s) 273, 276
TseI GCWGC 4 cut(s) 4, 60, 69, 93
TspGWI ACGGA 1 cut(s) 309
XceI RCATGY 2 cut(s) 16, 105
XspI CTAG 1 cut(s) 325
Zsp2I ATGCAT 2 cut(s) 18, 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.