MD15G1071500.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003322
Physical Location & Seq
Forward (+)
4914671 .. 4915677
1007 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1071500.v1.1.491

Sequence Viewer

Length: 681 bp
ATGAGCTGCAACGGGTGCCGAGTACTCCGGAAGGGATGCAGCGAGACATGCATACTCAGGCCGTGCCTGCACTGGATCGACTCTCCCCAAGCCCAAGCCAACGCCACCCTGTTCCTCGCCAAGTTCTTCGGCCGCAGCGACCTCCTCTCTTTCGTCTCCGCCGTACCGGAAACCCAACGCCCCGCTCTGTTTCAGTCTCTGCTTTTCGAAGCGTGTGGCCGCTCGGTGAACCCGGTGAACGGAGCGGTGGGGCTTCTGTCGAGCGGGAACTGGCAAGTGTGCCAGTCGGCGGTCGAGACGGTACTCTCCGGAGGCGTTTTGAGGCCGTTACCGGCGTTTACTACGCCGAGCCTCGACGAGTCTTCGGACACGTTCAGCAGAGGCACATGCACAAAACAAAACCGCTACTATCAATCCGTACCGTCAGCGTCGGCGTACTATGGTGAGGAGCGAAGCCAGCCGTTCGAGCCGGCGGGGCTCTCTCTGAAGCCGAATCTCGCGGCGGGAGAGAGAGGAAGAGGGAGGGGAATGAGGAGCTACATAAGTGCGGCGATGGATCAGCGGCTGAGAGATACGGTATCGTTTGATTCGGAGGAGTCGGTGATGACTACGACAACGACGAGGTGCCTTGAAAGCGGCGGCGATCTGAAGGATTGCAAGCTTCTGAACCTTTTCGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

227

Amino Acids

24.55

Weight (kDa)

8.03

Isoelectric Point (pI)

57.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 2 - 100 3.7e-25 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 15, 624
AccBSI CCGCTC 4 cut(s) 185, 222, 245, 264
AccII CGCG 1 cut(s) 500
AccIII TCCGGA 2 cut(s) 27, 308
AclWI GGATC 2 cut(s) 83, 564
AcoI YGGCCR 2 cut(s) 130, 217
AcuI CTGAAG 2 cut(s) 506, 668
AfaI GTAC 5 cut(s) 24, 165, 303, 420, 437
AfiI CCNNNNNNNGG 2 cut(s) 239, 289
AflIII ACRYGT 1 cut(s) 369
AgsI TTSAA 1 cut(s) 632
AluBI AGCT 3 cut(s) 6, 537, 661
AluI AGCT 3 cut(s) 6, 537, 661
Alw26I GTCTC 4 cut(s) 38, 160, 201, 290
AlwI GGATC 2 cut(s) 83, 564
AlwNI CAGNNNCTG 2 cut(s) 199, 565
Aor13HI TCCGGA 2 cut(s) 27, 308
AoxI GGCC 4 cut(s) 59, 130, 217, 323
ApeKI GCWGC 3 cut(s) 6, 39, 135
Asp700I GAANNNNTTC 1 cut(s) 671
AsuC2I CCSGG 1 cut(s) 233
AsuHPI GGTGA 4 cut(s) 238, 247, 455, 613
AsuII TTCGAA 1 cut(s) 207
BanI GGYRCC 2 cut(s) 15, 624
BanII GRGCYC 1 cut(s) 480
BbsI GAAGAC 1 cut(s) 354
BbvI GCAGC 2 cut(s) 51, 147
BccI CCATC 1 cut(s) 547
BceAI ACGGC 4 cut(s) 46, 146, 310, 445
BcnI CCSGG 1 cut(s) 233
BcoDI GTCTC 4 cut(s) 38, 160, 201, 290
BglI GCCNNNNNGGC 1 cut(s) 475
BmcAI AGTACT 1 cut(s) 24
Bme1390I CCNGG 1 cut(s) 233
BmiI GGNNCC 2 cut(s) 17, 626
BmrFI CCNGG 1 cut(s) 233
BmsI GCATC 1 cut(s) 26
BpiI GAAGAC 1 cut(s) 354
Bpu14I TTCGAA 1 cut(s) 207
BpuMI CCSGG 1 cut(s) 233
BsaWI WCCGGW 3 cut(s) 27, 166, 308
Bsc4I CCNNNNNNNGG 2 cut(s) 239, 289
Bse118I RCCGGY 2 cut(s) 331, 469
Bse1I ACTGG 3 cut(s) 77, 275, 283
BseAI TCCGGA 2 cut(s) 27, 308
BseGI GGATG 1 cut(s) 41
BseLI CCNNNNNNNGG 2 cut(s) 239, 289
BseMII CTCAG 2 cut(s) 70, 557
BseNI ACTGG 3 cut(s) 77, 275, 283
BseRI GAGGAG 4 cut(s) 134, 461, 547, 608
BseX3I CGGCCG 1 cut(s) 130
BseXI GCAGC 2 cut(s) 51, 147
BsgI GTGCAG 1 cut(s) 53
Bsh1236I CGCG 1 cut(s) 500
Bsh1285I CGRYCG 2 cut(s) 133, 294
BshFI GGCC 4 cut(s) 61, 132, 219, 325
BshNI GGYRCC 2 cut(s) 15, 624
BsiEI CGRYCG 2 cut(s) 133, 294
BsiSI CCGG 6 cut(s) 28, 167, 233, 309, 332, 470
BslI CCNNNNNNNGG 2 cut(s) 239, 289
BsmAI GTCTC 4 cut(s) 38, 160, 201, 290
BsmBI CGTCTC 2 cut(s) 160, 290
BsnI GGCC 4 cut(s) 61, 132, 219, 325
Bsp119I TTCGAA 1 cut(s) 207
Bsp1286I GDGCHC 1 cut(s) 480
Bsp13I TCCGGA 2 cut(s) 27, 308
Bsp143I GATC 3 cut(s) 75, 556, 643
BspANI GGCC 4 cut(s) 61, 132, 219, 325
BspCNI CTCAG 2 cut(s) 69, 558
BspEI TCCGGA 2 cut(s) 27, 308
BspFNI CGCG 1 cut(s) 500
BspLI GGNNCC 2 cut(s) 17, 626
BspPI GGATC 2 cut(s) 83, 564
BspT104I TTCGAA 1 cut(s) 207
BspT107I GGYRCC 2 cut(s) 15, 624
BsrBI CCGCTC 4 cut(s) 185, 222, 245, 264
BsrFI RCCGGY 2 cut(s) 331, 469
BsrI ACTGG 3 cut(s) 77, 275, 283
BssAI RCCGGY 2 cut(s) 331, 469
BssMI GATC 3 cut(s) 75, 556, 643
Bst4CI ACNGT 3 cut(s) 301, 423, 577
Bst6I CTCTTC 1 cut(s) 511
BstAPI GCANNNNNTGC 1 cut(s) 15
BstBI TTCGAA 1 cut(s) 207
BstC8I GCNNGC 4 cut(s) 68, 458, 471, 659
BstDEI CTNAG 2 cut(s) 56, 566
BstF5I GGATG 1 cut(s) 41
BstFNI CGCG 1 cut(s) 500
BstKTI GATC 3 cut(s) 78, 559, 646
BstMAI GTCTC 4 cut(s) 38, 160, 201, 290
BstMBI GATC 3 cut(s) 75, 556, 643
BstMCI CGRYCG 2 cut(s) 133, 294
BstMWI GCNNNNNNNGC 7 cut(s) 15, 48, 67, 457, 466, 475, 633
BstNSI RCATGY 2 cut(s) 51, 390
BstSCI CCNGG 1 cut(s) 231
BstUI CGCG 1 cut(s) 500
BstV1I GCAGC 2 cut(s) 51, 147
BstV2I GAAGAC 1 cut(s) 354
BstZI CGGCCG 1 cut(s) 130
BsuRI GGCC 4 cut(s) 61, 132, 219, 325
BtgZI GCGATG 1 cut(s) 566
BtsCI GGATG 1 cut(s) 41
BtsIMutI CAGTG 1 cut(s) 70
Cac8I GCNNGC 4 cut(s) 68, 458, 471, 659
CaiI CAGNNNCTG 2 cut(s) 199, 565
Cfr10I RCCGGY 2 cut(s) 331, 469
CseI GACGC 1 cut(s) 417
Csp6I GTAC 5 cut(s) 23, 164, 302, 419, 436
CviAII CATG 2 cut(s) 48, 387
CviQI GTAC 5 cut(s) 23, 164, 302, 419, 436
DdeI CTNAG 2 cut(s) 56, 566
DpnI GATC 3 cut(s) 77, 558, 645
DpnII GATC 3 cut(s) 75, 556, 643
EaeI YGGCCR 2 cut(s) 130, 217
EagI CGGCCG 1 cut(s) 130
Eam1104I CTCTTC 1 cut(s) 511
EarI CTCTTC 1 cut(s) 511
EciI GGCGGA 1 cut(s) 148
EclXI CGGCCG 1 cut(s) 130
Eco24I GRGCYC 1 cut(s) 480
Eco52I CGGCCG 1 cut(s) 130
Eco57I CTGAAG 2 cut(s) 506, 668
EcoT22I ATGCAT 1 cut(s) 53
EcoT38I GRGCYC 1 cut(s) 480
Esp3I CGTCTC 2 cut(s) 160, 290
FaeI CATG 2 cut(s) 51, 390
FaiI YATR 5 cut(s) 49, 53, 388, 441, 542
FatI CATG 2 cut(s) 47, 386
FauI CCCGC 4 cut(s) 190, 257, 466, 496
FokI GGATG 1 cut(s) 48
FriOI GRGCYC 1 cut(s) 480
HaeIII GGCC 4 cut(s) 61, 132, 219, 325
HapII CCGG 6 cut(s) 28, 167, 233, 309, 332, 470
HgaI GACGC 1 cut(s) 417
Hin1II CATG 2 cut(s) 51, 390
HindIII AAGCTT 1 cut(s) 659
HinfI GANTC 5 cut(s) 80, 359, 493, 587, 596
HpaII CCGG 6 cut(s) 28, 167, 233, 309, 332, 470
HphI GGTGA 4 cut(s) 238, 247, 455, 613
Hpy166II GTNNAC 3 cut(s) 229, 238, 339
Hpy188I TCNGA 5 cut(s) 367, 486, 592, 648, 666
Hpy188III TCNNGA 3 cut(s) 28, 295, 309
Hpy8I GTNNAC 3 cut(s) 229, 238, 339
Hpy99I CGWCG 3 cut(s) 359, 433, 622
HpyAV CCTTC 2 cut(s) 25, 643
HpyCH4III ACNGT 3 cut(s) 301, 423, 577
HpyCH4IV ACGT 1 cut(s) 371
HpyCH4V TGCA 6 cut(s) 9, 39, 51, 70, 390, 657
HpyF10VI GCNNNNNNNGC 7 cut(s) 15, 48, 67, 457, 466, 475, 633
HpyF3I CTNAG 2 cut(s) 56, 566
HpySE526I ACGT 1 cut(s) 371
Hsp92II CATG 2 cut(s) 51, 390
Kpn2I TCCGGA 2 cut(s) 27, 308
KroI GCCGGC 1 cut(s) 469
KroNI GCCGGC 1 cut(s) 471
Kzo9I GATC 3 cut(s) 75, 556, 643
LmnI GCTCC 3 cut(s) 242, 448, 534
Lsp1109I GCAGC 2 cut(s) 51, 147
LweI GCATC 1 cut(s) 26
MaeII ACGT 1 cut(s) 371
MaeIII GTNAC 1 cut(s) 327
MalI GATC 3 cut(s) 77, 558, 645
MbiI CCGCTC 4 cut(s) 185, 222, 245, 264
MboI GATC 3 cut(s) 75, 556, 643
MboII GAAGA 3 cut(s) 118, 354, 528
MhlI GDGCHC 1 cut(s) 480
MlyI GAGTC 3 cut(s) 74, 368, 605
Mph1103I ATGCAT 1 cut(s) 53
MroI TCCGGA 2 cut(s) 27, 308
MroNI GCCGGC 1 cut(s) 469
MroXI GAANNNNTTC 1 cut(s) 671
MspA1I CMGCKG 1 cut(s) 562
MspI CCGG 6 cut(s) 28, 167, 233, 309, 332, 470
MspR9I CCNGG 1 cut(s) 233
MvnI CGCG 1 cut(s) 500
MwoI GCNNNNNNNGC 7 cut(s) 15, 48, 67, 457, 466, 475, 633
NaeI GCCGGC 1 cut(s) 471
NciI CCSGG 1 cut(s) 233
NdeII GATC 3 cut(s) 75, 556, 643
NgoMIV GCCGGC 1 cut(s) 469
NlaIII CATG 2 cut(s) 51, 390
NlaIV GGNNCC 2 cut(s) 17, 626
NmeAIII GCCGAG 2 cut(s) 44, 372
NsiI ATGCAT 1 cut(s) 53
NspI RCATGY 2 cut(s) 51, 390
NspV TTCGAA 1 cut(s) 207
PcsI WCGNNNNNNNCGW 3 cut(s) 135, 159, 617
PdiI GCCGGC 1 cut(s) 471
PdmI GAANNNNTTC 1 cut(s) 671
PfeI GAWTC 2 cut(s) 493, 587
PleI GAGTC 3 cut(s) 74, 367, 604
PpsI GAGTC 3 cut(s) 74, 367, 604
PspN4I GGNNCC 2 cut(s) 17, 626
PstNI CAGNNNCTG 2 cut(s) 199, 565
RsaI GTAC 5 cut(s) 24, 165, 303, 420, 437
RsaNI GTAC 5 cut(s) 23, 164, 302, 419, 436
Sau3AI GATC 3 cut(s) 75, 556, 643
ScaI AGTACT 1 cut(s) 24
SchI GAGTC 3 cut(s) 74, 368, 605
ScrFI CCNGG 1 cut(s) 233
SduI GDGCHC 1 cut(s) 480
SetI ASST 7 cut(s) 8, 144, 374, 539, 626, 663, 672
SfaNI GCATC 1 cut(s) 26
SfuI TTCGAA 1 cut(s) 207
StyD4I CCNGG 1 cut(s) 231
TaaI ACNGT 3 cut(s) 301, 423, 577
TaiI ACGT 1 cut(s) 374
TaqI TCGA 6 cut(s) 78, 207, 260, 294, 354, 465
TatI WGTACW 1 cut(s) 22
TauI GCSGC 7 cut(s) 135, 222, 503, 551, 565, 639, 642
TfiI GAWTC 2 cut(s) 493, 587
TscAI CASTG 1 cut(s) 77
TseI GCWGC 3 cut(s) 6, 39, 135
TspGWI ACGGA 2 cut(s) 255, 406
TspRI CASTG 1 cut(s) 77
XceI RCATGY 2 cut(s) 51, 390
XmnI GAANNNNTTC 1 cut(s) 671
ZrmI AGTACT 1 cut(s) 24
Zsp2I ATGCAT 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.