pycom08g07110

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003322
Physical Location & Seq
Forward (+)
5729766 .. 5730549
784 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g07110.1

Sequence Viewer

Length: 675 bp
ATGAGCTGCAACGGCTGCCGAGTGCTCCGGAAGGGATGCAGCGACACATGCATACTGAGGTCGTGCCTGTACTGGATCGACTCTCCCGAAGCCCAAGGCCACGCCACCCTGTTCCTTGCCAAGTTCTTTGGCCGCAGCGATCTACTCTCCTTCGTCTCCGCCGTACCGGAAAACCAACGCCCCGCTCTGTTTCAGTCTCTGCTGTTCGAAGCGTGCGGCCGCACGGTGAACCCGGTGAACGGAGCGGTGGGGCTTCTGTCGAGCGGGAACTGGCACGTGTGCCAGTCGGCGGTCGAAACGGTGCTCTGCGGCGGCGCTTTGAGGCCGTTACAGGCTTTCACTACGCCTACCCTCGACGAGTCTTCGGACGCGTTCAGCAGAGGCGCATGCACGCCGAAAAACCGCTACTATCCGTCGGCGTCGGCATACTACGACGCGGAGCGACTCCAGCCGTTCGAGGCGGCGGGGCTCTCTCTGAAGCCGAATCTCGTGGCGGCAGAGAGAGGAAGAGGGAGGGGAATGTGGATCTGCGTAAGTGCCGCGATGGATCAGCGGCGGAGGGATACGGTGTCGTTTGATTCGGAGGAGTCGGTGATGACTACGACAACGACGAGGACCTTTGAAAGCGGTGGCGGCGATCTGAAGGATCGCAAGCTTCTGAACCTTTTTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

225

Amino Acids

24.43

Weight (kDa)

8.04

Isoelectric Point (pI)

55.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 2 - 100 7.9e-24 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 185, 245, 264
AccII CGCG 3 cut(s) 371, 437, 542
AccIII TCCGGA 1 cut(s) 27
AclWI GGATC 4 cut(s) 83, 533, 555, 654
AcoI YGGCCR 2 cut(s) 130, 217
AcuI CTGAAG 2 cut(s) 497, 662
AcvI CACGTG 1 cut(s) 277
AcyI GRCGYC 1 cut(s) 419
AfaI GTAC 2 cut(s) 71, 165
AfiI CCNNNNNNNGG 2 cut(s) 239, 289
AflIII ACRYGT 2 cut(s) 276, 369
AgsI TTSAA 1 cut(s) 623
AluBI AGCT 2 cut(s) 6, 655
AluI AGCT 2 cut(s) 6, 655
Alw21I GWGCWC 2 cut(s) 27, 306
Alw26I GTCTC 2 cut(s) 160, 201
AlwI GGATC 4 cut(s) 83, 533, 555, 654
AlwNI CAGNNNCTG 1 cut(s) 199
Aor13HI TCCGGA 1 cut(s) 27
AoxI GGCC 4 cut(s) 97, 130, 217, 323
ApeKI GCWGC 4 cut(s) 6, 15, 39, 135
AspLEI GCGC 2 cut(s) 317, 386
AspS9I GGNCC 1 cut(s) 615
AsuC2I CCSGG 1 cut(s) 233
AsuHPI GGTGA 3 cut(s) 238, 247, 604
AsuII TTCGAA 1 cut(s) 207
AvaII GGWCC 1 cut(s) 615
BanII GRGCYC 1 cut(s) 471
BauI CACGAG 1 cut(s) 488
BbrPI CACGTG 1 cut(s) 277
BbsI GAAGAC 1 cut(s) 354
Bbv12I GWGCWC 2 cut(s) 27, 306
BbvI GCAGC 3 cut(s) 2, 51, 147
BccI CCATC 1 cut(s) 538
BceAI ACGGC 4 cut(s) 28, 146, 310, 436
BciVI GTATCC 1 cut(s) 556
BcnI CCSGG 1 cut(s) 233
BcoDI GTCTC 2 cut(s) 160, 201
BfoI RGCGCY 1 cut(s) 318
BfuI GTATCC 1 cut(s) 556
Bme1390I CCNGG 1 cut(s) 233
Bme18I GGWCC 1 cut(s) 615
BmgT120I GGNCC 1 cut(s) 615
BmrFI CCNGG 1 cut(s) 233
BmsI GCATC 1 cut(s) 26
BpiI GAAGAC 1 cut(s) 354
BpmI CTGGAG 1 cut(s) 431
Bpu14I TTCGAA 1 cut(s) 207
BpuMI CCSGG 1 cut(s) 233
BsaAI YACGTR 1 cut(s) 277
BsaHI GRCGYC 1 cut(s) 419
BsaJI CCNNGG 1 cut(s) 94
BsaWI WCCGGW 2 cut(s) 27, 166
Bsc4I CCNNNNNNNGG 2 cut(s) 239, 289
Bse1I ACTGG 3 cut(s) 77, 275, 283
BseAI TCCGGA 1 cut(s) 27
BseDI CCNNGG 1 cut(s) 94
BseGI GGATG 1 cut(s) 41
BseLI CCNNNNNNNGG 2 cut(s) 239, 289
BseMII CTCAG 1 cut(s) 47
BseNI ACTGG 3 cut(s) 77, 275, 283
BseRI GAGGAG 1 cut(s) 599
BseX3I CGGCCG 1 cut(s) 217
BseXI GCAGC 3 cut(s) 2, 51, 147
Bsh1236I CGCG 3 cut(s) 371, 437, 542
Bsh1285I CGRYCG 2 cut(s) 220, 294
BshFI GGCC 4 cut(s) 99, 132, 219, 325
BsiEI CGRYCG 2 cut(s) 220, 294
BsiHKAI GWGCWC 2 cut(s) 27, 306
BsiSI CCGG 3 cut(s) 28, 167, 233
BslI CCNNNNNNNGG 2 cut(s) 239, 289
BsmAI GTCTC 2 cut(s) 160, 201
BsmBI CGTCTC 1 cut(s) 160
BsnI GGCC 4 cut(s) 99, 132, 219, 325
Bsp119I TTCGAA 1 cut(s) 207
Bsp1286I GDGCHC 3 cut(s) 27, 306, 471
Bsp13I TCCGGA 1 cut(s) 27
Bsp143I GATC 6 cut(s) 75, 139, 525, 547, 637, 646
BspANI GGCC 4 cut(s) 99, 132, 219, 325
BspCNI CTCAG 1 cut(s) 48
BspEI TCCGGA 1 cut(s) 27
BspFNI CGCG 3 cut(s) 371, 437, 542
BspPI GGATC 4 cut(s) 83, 533, 555, 654
BspT104I TTCGAA 1 cut(s) 207
BsrBI CCGCTC 3 cut(s) 185, 245, 264
BsrI ACTGG 3 cut(s) 77, 275, 283
BssECI CCNNGG 1 cut(s) 94
BssMI GATC 6 cut(s) 75, 139, 525, 547, 637, 646
BssNI GRCGYC 1 cut(s) 419
BssSI CACGAG 1 cut(s) 488
BssT1I CCWWGG 1 cut(s) 94
Bst2BI CACGAG 1 cut(s) 488
Bst4CI ACNGT 3 cut(s) 226, 301, 568
Bst6I CTCTTC 1 cut(s) 502
BstACI GRCGYC 1 cut(s) 419
BstAPI GCANNNNNTGC 1 cut(s) 15
BstBAI YACGTR 1 cut(s) 277
BstBI TTCGAA 1 cut(s) 207
BstC8I GCNNGC 4 cut(s) 214, 388, 392, 653
BstDEI CTNAG 1 cut(s) 56
BstF5I GGATG 1 cut(s) 41
BstFNI CGCG 3 cut(s) 371, 437, 542
BstH2I RGCGCY 1 cut(s) 318
BstHHI GCGC 2 cut(s) 317, 386
BstKTI GATC 6 cut(s) 78, 142, 528, 550, 640, 649
BstMAI GTCTC 2 cut(s) 160, 201
BstMBI GATC 6 cut(s) 75, 139, 525, 547, 637, 646
BstMCI CGRYCG 2 cut(s) 220, 294
BstMWI GCNNNNNNNGC 5 cut(s) 12, 15, 48, 448, 633
BstNSI RCATGY 2 cut(s) 51, 390
BstSCI CCNGG 1 cut(s) 231
BstUI CGCG 3 cut(s) 371, 437, 542
BstV1I GCAGC 3 cut(s) 2, 51, 147
BstV2I GAAGAC 1 cut(s) 354
BstX2I RGATCY 1 cut(s) 525
BstYI RGATCY 1 cut(s) 525
BstZI CGGCCG 1 cut(s) 217
BsuI GTATCC 1 cut(s) 556
BsuRI GGCC 4 cut(s) 99, 132, 219, 325
BtgZI GCGATG 1 cut(s) 557
BtsCI GGATG 1 cut(s) 41
Cac8I GCNNGC 4 cut(s) 214, 388, 392, 653
CaiI CAGNNNCTG 1 cut(s) 199
CciNI GCGGCCGC 1 cut(s) 217
CfoI GCGC 2 cut(s) 317, 386
Cfr13I GGNCC 1 cut(s) 615
CseI GACGC 3 cut(s) 377, 408, 443
Csp6I GTAC 2 cut(s) 70, 164
CviAII CATG 2 cut(s) 48, 387
CviQI GTAC 2 cut(s) 70, 164
DdeI CTNAG 1 cut(s) 56
DpnI GATC 6 cut(s) 77, 141, 527, 549, 639, 648
DpnII GATC 6 cut(s) 75, 139, 525, 547, 637, 646
EaeI YGGCCR 2 cut(s) 130, 217
EagI CGGCCG 1 cut(s) 217
Eam1104I CTCTTC 1 cut(s) 502
EarI CTCTTC 1 cut(s) 502
EciI GGCGGA 2 cut(s) 148, 571
EclXI CGGCCG 1 cut(s) 217
Eco130I CCWWGG 1 cut(s) 94
Eco24I GRGCYC 1 cut(s) 471
Eco47I GGWCC 1 cut(s) 615
Eco52I CGGCCG 1 cut(s) 217
Eco57I CTGAAG 2 cut(s) 497, 662
Eco72I CACGTG 1 cut(s) 277
EcoO109I RGGNCCY 1 cut(s) 615
EcoT14I CCWWGG 1 cut(s) 94
EcoT22I ATGCAT 1 cut(s) 53
EcoT38I GRGCYC 1 cut(s) 471
ErhI CCWWGG 1 cut(s) 94
Esp3I CGTCTC 1 cut(s) 160
FaeI CATG 2 cut(s) 51, 390
FaiI YATR 4 cut(s) 49, 53, 388, 427
FatI CATG 2 cut(s) 47, 386
FauI CCCGC 3 cut(s) 190, 257, 457
FokI GGATG 1 cut(s) 48
FriOI GRGCYC 1 cut(s) 471
GlaI GCGC 2 cut(s) 316, 385
GsuI CTGGAG 1 cut(s) 431
HaeII RGCGCY 1 cut(s) 318
HaeIII GGCC 4 cut(s) 99, 132, 219, 325
HapII CCGG 3 cut(s) 28, 167, 233
HgaI GACGC 3 cut(s) 377, 408, 443
HhaI GCGC 2 cut(s) 317, 386
Hin1I GRCGYC 1 cut(s) 419
Hin1II CATG 2 cut(s) 51, 390
Hin6I GCGC 2 cut(s) 315, 384
HinP1I GCGC 2 cut(s) 315, 384
HindIII AAGCTT 1 cut(s) 653
HinfI GANTC 6 cut(s) 80, 359, 444, 484, 578, 587
HpaII CCGG 3 cut(s) 28, 167, 233
HphI GGTGA 3 cut(s) 238, 247, 604
Hpy166II GTNNAC 2 cut(s) 229, 238
Hpy188I TCNGA 5 cut(s) 367, 477, 583, 642, 660
Hpy188III TCNNGA 2 cut(s) 28, 86
Hpy8I GTNNAC 2 cut(s) 229, 238
Hpy99I CGWCG 5 cut(s) 359, 418, 424, 437, 613
HpyAV CCTTC 3 cut(s) 25, 160, 637
HpyCH4III ACNGT 3 cut(s) 226, 301, 568
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 4 cut(s) 9, 39, 51, 390
HpyF10VI GCNNNNNNNGC 5 cut(s) 12, 15, 48, 448, 633
HpyF3I CTNAG 1 cut(s) 56
HpySE526I ACGT 1 cut(s) 276
Hsp92I GRCGYC 1 cut(s) 419
Hsp92II CATG 2 cut(s) 51, 390
HspAI GCGC 2 cut(s) 315, 384
Kpn2I TCCGGA 1 cut(s) 27
Kzo9I GATC 6 cut(s) 75, 139, 525, 547, 637, 646
LmnI GCTCC 3 cut(s) 30, 242, 439
Lsp1109I GCAGC 3 cut(s) 2, 51, 147
LweI GCATC 1 cut(s) 26
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 1 cut(s) 327
MalI GATC 6 cut(s) 77, 141, 527, 549, 639, 648
MbiI CCGCTC 3 cut(s) 185, 245, 264
MboI GATC 6 cut(s) 75, 139, 525, 547, 637, 646
MboII GAAGA 2 cut(s) 354, 519
MflI RGATCY 1 cut(s) 525
MhlI GDGCHC 3 cut(s) 27, 306, 471
MluI ACGCGT 1 cut(s) 369
MlyI GAGTC 4 cut(s) 74, 368, 438, 596
Mph1103I ATGCAT 1 cut(s) 53
MroI TCCGGA 1 cut(s) 27
MspA1I CMGCKG 1 cut(s) 553
MspI CCGG 3 cut(s) 28, 167, 233
MspR9I CCNGG 1 cut(s) 233
MvnI CGCG 3 cut(s) 371, 437, 542
MwoI GCNNNNNNNGC 5 cut(s) 12, 15, 48, 448, 633
NciI CCSGG 1 cut(s) 233
NdeII GATC 6 cut(s) 75, 139, 525, 547, 637, 646
NlaIII CATG 2 cut(s) 51, 390
NmeAIII GCCGAG 1 cut(s) 44
NotI GCGGCCGC 1 cut(s) 217
NsiI ATGCAT 1 cut(s) 53
NspI RCATGY 2 cut(s) 51, 390
NspV TTCGAA 1 cut(s) 207
PaeI GCATGC 1 cut(s) 390
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 159, 608
PfeI GAWTC 2 cut(s) 484, 578
PleI GAGTC 4 cut(s) 74, 367, 438, 595
PmaCI CACGTG 1 cut(s) 277
PmlI CACGTG 1 cut(s) 277
PpsI GAGTC 4 cut(s) 74, 367, 438, 595
Ppu21I YACGTR 1 cut(s) 277
PpuMI RGGWCCY 1 cut(s) 615
Psp5II RGGWCCY 1 cut(s) 615
PspCI CACGTG 1 cut(s) 277
PspPI GGNCC 1 cut(s) 615
PspPPI RGGWCCY 1 cut(s) 615
PstNI CAGNNNCTG 1 cut(s) 199
PsuI RGATCY 1 cut(s) 525
RsaI GTAC 2 cut(s) 71, 165
RsaNI GTAC 2 cut(s) 70, 164
Sau3AI GATC 6 cut(s) 75, 139, 525, 547, 637, 646
Sau96I GGNCC 1 cut(s) 615
SchI GAGTC 4 cut(s) 74, 368, 438, 596
ScrFI CCNGG 1 cut(s) 233
SduI GDGCHC 3 cut(s) 27, 306, 471
SetI ASST 6 cut(s) 8, 62, 279, 620, 657, 666
SfaNI GCATC 1 cut(s) 26
SfuI TTCGAA 1 cut(s) 207
SinI GGWCC 1 cut(s) 615
SphI GCATGC 1 cut(s) 390
StyD4I CCNGG 1 cut(s) 231
StyI CCWWGG 1 cut(s) 94
TaaI ACNGT 3 cut(s) 226, 301, 568
TaiI ACGT 1 cut(s) 279
TaqI TCGA 6 cut(s) 78, 207, 260, 294, 354, 456
TatI WGTACW 1 cut(s) 69
TfiI GAWTC 2 cut(s) 484, 578
TseI GCWGC 4 cut(s) 6, 15, 39, 135
TspGWI ACGGA 2 cut(s) 255, 402
VpaK11BI GGWCC 1 cut(s) 615
XceI RCATGY 2 cut(s) 51, 390
Zsp2I ATGCAT 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.