FvH4_2g33260

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003322
Physical Location & Seq
Forward (+)
24995288 .. 24997660
2373 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33260.t2

Sequence Viewer

Length: 645 bp
ATGAGCTGCAACGGTTGCCGAGTCCTACGGAAGGGGTGCAGTGAGACATGCGTACTGAGGTCCTGCCTCCGCTGGATCGATTCTCCCGAATCCCAAGGCTACGCCACCTTGTTCCTCGCCAAGTTTTTCGGCCGTAGCGACCTCTTGTCCTTCATCTCCGCCGTGCCTGAAAACCAACGCCCTGCTCTTTTTCAGTCTCTGCTTTTTGAAGCGTGCGGGCGTACGGTGAACCCGGTGAGCGGAGCGGTGGGGATTCTCTCGAGCGGGAACTGGCACGTGTGTCACGCGGCGGTCCAGACGGTGTTATCCGGCGGTGTTTTGAGGCCGTTACCGGCGATGCTGACGCCAAACCTGGACGAATCATCCCAAGTCACTATCAACAGAGGCTCATGCGGAATTCAAAACCCCCGCTACACATCCGTACCGTCCATGTTCGACGACGACGTGGAGCCGTTGGATGTAGGACTCTCGCTGAAGCCGAATCTCGCGGCGGCCGGAGGAAGAGGACGAGCGAGGGGATGGAGCTGTGCAGTTGATCGAATGAGAGACACGGTGTCGTTTAGCTCCGAAGATTCGGTCATGACTACTAGCGGTGGCGGCGGCGGTTCGGACGGTGGCCGCAAGCTTTTGAACCTTTTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

215

Amino Acids

22.72

Weight (kDa)

8.1

Isoelectric Point (pI)

55.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 2 - 100 1.1e-23 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 240, 245, 264
AccII CGCG 2 cut(s) 287, 488
AclWI GGATC 1 cut(s) 83
AcoI YGGCCR 3 cut(s) 130, 492, 616
AcsI RAATTY 1 cut(s) 396
AcuI CTGAAG 1 cut(s) 494
AcvI CACGTG 1 cut(s) 277
AcyI GRCGYC 1 cut(s) 344
AfaI GTAC 3 cut(s) 54, 223, 423
AfiI CCNNNNNNNGG 2 cut(s) 31, 239
AflIII ACRYGT 1 cut(s) 276
AgsI TTSAA 3 cut(s) 209, 401, 631
AhdI GACNNNNNGTC 2 cut(s) 145, 553
AjiI CACGTC 1 cut(s) 445
AjnI CCWGG 1 cut(s) 351
AluBI AGCT 4 cut(s) 6, 525, 564, 625
AluI AGCT 4 cut(s) 6, 525, 564, 625
Alw26I GTCTC 3 cut(s) 38, 201, 540
AlwI GGATC 1 cut(s) 83
AlwNI CAGNNNCTG 1 cut(s) 199
Ama87I CYCGRG 1 cut(s) 259
AoxI GGCC 4 cut(s) 130, 323, 492, 616
ApeKI GCWGC 1 cut(s) 6
ApoI RAATTY 1 cut(s) 396
ArsI GACNNNNNNTTYG 2 cut(s) 561, 593
AspS9I GGNCC 2 cut(s) 60, 292
AsuC2I CCSGG 1 cut(s) 233
AsuHPI GGTGA 2 cut(s) 238, 247
AvaI CYCGRG 1 cut(s) 259
AvaII GGWCC 2 cut(s) 60, 292
BaeI ACNNNNGTAYC 2 cut(s) 405, 438
BbrPI CACGTG 1 cut(s) 277
BccI CCATC 1 cut(s) 513
BceAI ACGGC 4 cut(s) 117, 146, 310, 436
BciT130I CCWGG 1 cut(s) 353
BcnI CCSGG 1 cut(s) 233
BcoDI GTCTC 3 cut(s) 38, 201, 540
BfaI CTAG 1 cut(s) 588
BisI GCNGC 7 cut(s) 7, 288, 489, 492, 598, 601, 619
BlsI GCNGC 7 cut(s) 8, 289, 490, 493, 599, 602, 620
Bme1390I CCNGG 2 cut(s) 233, 353
Bme18I GGWCC 2 cut(s) 60, 292
BmeRI GACNNNNNGTC 2 cut(s) 145, 553
BmeT110I CYCGRG 1 cut(s) 259
BmgBI CACGTC 1 cut(s) 445
BmgT120I GGNCC 2 cut(s) 60, 292
BmiI GGNNCC 1 cut(s) 450
BmrFI CCNGG 2 cut(s) 233, 353
BmsI GCATC 1 cut(s) 327
BpuMI CCSGG 1 cut(s) 233
Bsa29I ATCGAT 1 cut(s) 78
BsaAI YACGTR 1 cut(s) 277
BsaHI GRCGYC 1 cut(s) 344
BsaJI CCNNGG 1 cut(s) 94
Bsc4I CCNNNNNNNGG 2 cut(s) 31, 239
Bse118I RCCGGY 1 cut(s) 331
Bse1I ACTGG 1 cut(s) 275
BseBI CCWGG 1 cut(s) 353
BseCI ATCGAT 1 cut(s) 78
BseDI CCNNGG 1 cut(s) 94
BseGI GGATG 4 cut(s) 362, 416, 463, 524
BseLI CCNNNNNNNGG 2 cut(s) 31, 239
BseMII CTCAG 1 cut(s) 47
BseNI ACTGG 1 cut(s) 275
BseX3I CGGCCG 2 cut(s) 130, 492
BsgI GTGCAG 2 cut(s) 58, 549
Bsh1236I CGCG 2 cut(s) 287, 488
Bsh1285I CGRYCG 2 cut(s) 133, 495
BshFI GGCC 4 cut(s) 132, 325, 494, 618
BshVI ATCGAT 1 cut(s) 78
BsiEI CGRYCG 2 cut(s) 133, 495
BsiHKCI CYCGRG 1 cut(s) 259
BsiSI CCGG 4 cut(s) 233, 309, 332, 495
BsiWI CGTACG 1 cut(s) 221
BslI CCNNNNNNNGG 2 cut(s) 31, 239
BsmAI GTCTC 3 cut(s) 38, 201, 540
BsnI GGCC 4 cut(s) 132, 325, 494, 618
BsoBI CYCGRG 1 cut(s) 259
Bsp143I GATC 2 cut(s) 75, 535
BspANI GGCC 4 cut(s) 132, 325, 494, 618
BspCNI CTCAG 1 cut(s) 48
BspDI ATCGAT 1 cut(s) 78
BspFNI CGCG 2 cut(s) 287, 488
BspHI TCATGA 1 cut(s) 579
BspLI GGNNCC 1 cut(s) 450
BspPI GGATC 1 cut(s) 83
BsrBI CCGCTC 3 cut(s) 240, 245, 264
BsrFI RCCGGY 1 cut(s) 331
BsrI ACTGG 1 cut(s) 275
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 1 cut(s) 94
BssMI GATC 2 cut(s) 75, 535
BssNI GRCGYC 1 cut(s) 344
BssT1I CCWWGG 1 cut(s) 94
Bst2UI CCWGG 1 cut(s) 353
Bst4CI ACNGT 6 cut(s) 14, 226, 301, 426, 553, 614
Bst6I CTCTTC 1 cut(s) 496
BstACI GRCGYC 1 cut(s) 344
BstAPI GCANNNNNTGC 1 cut(s) 15
BstBAI YACGTR 1 cut(s) 277
BstC8I GCNNGC 3 cut(s) 214, 218, 623
BstDEI CTNAG 1 cut(s) 56
BstENI CCTNNNNNAGG 1 cut(s) 29
BstF5I GGATG 4 cut(s) 362, 416, 463, 524
BstFNI CGCG 2 cut(s) 287, 488
BstKTI GATC 2 cut(s) 78, 538
BstMAI GTCTC 3 cut(s) 38, 201, 540
BstMBI GATC 2 cut(s) 75, 535
BstMCI CGRYCG 2 cut(s) 133, 495
BstMWI GCNNNNNNNGC 2 cut(s) 15, 597
BstNI CCWGG 1 cut(s) 353
BstNSI RCATGY 1 cut(s) 51
BstSCI CCNGG 2 cut(s) 231, 351
BstUI CGCG 2 cut(s) 287, 488
BstZI CGGCCG 2 cut(s) 130, 492
Bsu15I ATCGAT 1 cut(s) 78
BsuRI GGCC 4 cut(s) 132, 325, 494, 618
BsuTUI ATCGAT 1 cut(s) 78
BtgZI GCGATG 1 cut(s) 350
BtrI CACGTC 1 cut(s) 445
BtsCI GGATG 4 cut(s) 362, 416, 463, 524
BtsI GCAGTG 1 cut(s) 46
BtsIMutI CAGTG 1 cut(s) 46
Cac8I GCNNGC 3 cut(s) 214, 218, 623
CaiI CAGNNNCTG 1 cut(s) 199
CciI TCATGA 1 cut(s) 579
Cfr10I RCCGGY 1 cut(s) 331
Cfr13I GGNCC 2 cut(s) 60, 292
ClaI ATCGAT 1 cut(s) 78
CseI GACGC 1 cut(s) 352
Csp6I GTAC 3 cut(s) 53, 222, 422
CviAII CATG 4 cut(s) 48, 390, 430, 580
CviQI GTAC 3 cut(s) 53, 222, 422
DdeI CTNAG 1 cut(s) 56
DpnI GATC 2 cut(s) 77, 537
DpnII GATC 2 cut(s) 75, 535
DriI GACNNNNNGTC 2 cut(s) 145, 553
EaeI YGGCCR 3 cut(s) 130, 492, 616
EagI CGGCCG 2 cut(s) 130, 492
Eam1104I CTCTTC 1 cut(s) 496
Eam1105I GACNNNNNGTC 2 cut(s) 145, 553
EarI CTCTTC 1 cut(s) 496
EciI GGCGGA 1 cut(s) 148
EclXI CGGCCG 2 cut(s) 130, 492
Eco130I CCWWGG 1 cut(s) 94
Eco47I GGWCC 2 cut(s) 60, 292
Eco52I CGGCCG 2 cut(s) 130, 492
Eco57I CTGAAG 1 cut(s) 494
Eco72I CACGTG 1 cut(s) 277
Eco88I CYCGRG 1 cut(s) 259
EcoNI CCTNNNNNAGG 1 cut(s) 29
EcoO109I RGGNCCY 1 cut(s) 60
EcoRI GAATTC 1 cut(s) 396
EcoRII CCWGG 1 cut(s) 351
EcoT14I CCWWGG 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 94
FaeI CATG 4 cut(s) 51, 393, 433, 583
FaiI YATR 4 cut(s) 49, 391, 431, 581
FatI CATG 4 cut(s) 47, 389, 429, 579
FauI CCCGC 3 cut(s) 209, 257, 416
Fnu4HI GCNGC 7 cut(s) 7, 288, 489, 492, 598, 601, 619
FokI GGATG 4 cut(s) 349, 403, 470, 531
Fsp4HI GCNGC 7 cut(s) 7, 288, 489, 492, 598, 601, 619
FspBI CTAG 1 cut(s) 588
GluI GCNGC 7 cut(s) 7, 288, 489, 492, 598, 601, 619
HaeIII GGCC 4 cut(s) 132, 325, 494, 618
HapII CCGG 4 cut(s) 233, 309, 332, 495
HgaI GACGC 1 cut(s) 352
Hin1I GRCGYC 1 cut(s) 344
Hin1II CATG 4 cut(s) 51, 393, 433, 583
HindIII AAGCTT 1 cut(s) 623
HinfI GANTC 8 cut(s) 21, 80, 89, 253, 359, 465, 481, 572
HpaII CCGG 4 cut(s) 233, 309, 332, 495
HphI GGTGA 2 cut(s) 238, 247
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 2 cut(s) 568, 610
Hpy188III TCNNGA 4 cut(s) 86, 259, 295, 580
Hpy8I GTNNAC 1 cut(s) 229
Hpy99I CGWCG 3 cut(s) 440, 443, 446
HpyAV CCTTC 2 cut(s) 25, 160
HpyCH4III ACNGT 6 cut(s) 14, 226, 301, 426, 553, 614
HpyCH4IV ACGT 2 cut(s) 276, 444
HpyCH4V TGCA 3 cut(s) 9, 39, 530
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 597
HpyF3I CTNAG 1 cut(s) 56
HpySE526I ACGT 2 cut(s) 276, 444
Hsp92I GRCGYC 1 cut(s) 344
Hsp92II CATG 4 cut(s) 51, 393, 433, 583
Kzo9I GATC 2 cut(s) 75, 535
LmnI GCTCC 4 cut(s) 242, 448, 522, 569
LweI GCATC 1 cut(s) 327
MaeI CTAG 1 cut(s) 588
MaeII ACGT 2 cut(s) 276, 444
MaeIII GTNAC 3 cut(s) 281, 327, 370
MalI GATC 2 cut(s) 77, 537
MbiI CCGCTC 3 cut(s) 240, 245, 264
MboI GATC 2 cut(s) 75, 535
MboII GAAGA 2 cut(s) 513, 581
MluCI AATT 1 cut(s) 396
MlyI GAGTC 2 cut(s) 30, 459
MmeI TCCRAC 1 cut(s) 435
MnlI CCTC 9 cut(s) 51, 77, 125, 152, 315, 377, 491, 497, 507
MspA1I CMGCKG 1 cut(s) 72
MspI CCGG 4 cut(s) 233, 309, 332, 495
MspR9I CCNGG 2 cut(s) 233, 353
MvaI CCWGG 1 cut(s) 353
MvnI CGCG 2 cut(s) 287, 488
MwoI GCNNNNNNNGC 2 cut(s) 15, 597
NciI CCSGG 1 cut(s) 233
NdeII GATC 2 cut(s) 75, 535
NlaIII CATG 4 cut(s) 51, 393, 433, 583
NlaIV GGNNCC 1 cut(s) 450
NmeAIII GCCGAG 1 cut(s) 44
NmuCI GTSAC 2 cut(s) 281, 370
NspI RCATGY 1 cut(s) 51
PaeR7I CTCGAG 1 cut(s) 259
PagI TCATGA 1 cut(s) 579
PcsI WCGNNNNNNNCGW 4 cut(s) 84, 135, 441, 476
PfeI GAWTC 6 cut(s) 80, 89, 253, 359, 481, 572
Pfl23II CGTACG 1 cut(s) 221
PkrI GCNGC 7 cut(s) 8, 289, 490, 493, 599, 602, 620
PleI GAGTC 2 cut(s) 29, 459
PmaCI CACGTG 1 cut(s) 277
PmlI CACGTG 1 cut(s) 277
PpsI GAGTC 2 cut(s) 29, 459
Ppu21I YACGTR 1 cut(s) 277
PpuMI RGGWCCY 1 cut(s) 60
Psp5II RGGWCCY 1 cut(s) 60
Psp6I CCWGG 1 cut(s) 351
PspCI CACGTG 1 cut(s) 277
PspGI CCWGG 1 cut(s) 351
PspLI CGTACG 1 cut(s) 221
PspN4I GGNNCC 1 cut(s) 450
PspPI GGNCC 2 cut(s) 60, 292
PspPPI RGGWCCY 1 cut(s) 60
PstNI CAGNNNCTG 1 cut(s) 199
RsaI GTAC 3 cut(s) 54, 223, 423
RsaNI GTAC 3 cut(s) 53, 222, 422
SatI GCNGC 7 cut(s) 7, 288, 489, 492, 598, 601, 619
Sau3AI GATC 2 cut(s) 75, 535
Sau96I GGNCC 2 cut(s) 60, 292
SchI GAGTC 2 cut(s) 30, 459
ScrFI CCNGG 2 cut(s) 233, 353
SfaNI GCATC 1 cut(s) 327
Sfr274I CTCGAG 1 cut(s) 259
SinI GGWCC 2 cut(s) 60, 292
SlaI CTCGAG 1 cut(s) 259
SmlI CTYRAG 1 cut(s) 259
SmoI CTYRAG 1 cut(s) 259
Sse9I AATT 1 cut(s) 396
SspMI CTAG 1 cut(s) 588
StyD4I CCNGG 2 cut(s) 231, 351
StyI CCWWGG 1 cut(s) 94
TaaI ACNGT 6 cut(s) 14, 226, 301, 426, 553, 614
TaiI ACGT 2 cut(s) 279, 447
TaqI TCGA 4 cut(s) 78, 260, 435, 538
TaqII GACCGA 1 cut(s) 565
TasI AATT 1 cut(s) 396
TauI GCSGC 6 cut(s) 290, 491, 494, 600, 603, 621
TfiI GAWTC 6 cut(s) 80, 89, 253, 359, 481, 572
TscAI CASTG 1 cut(s) 46
TseFI GTSAC 2 cut(s) 281, 370
TseI GCWGC 1 cut(s) 6
Tsp45I GTSAC 2 cut(s) 281, 370
TspDTI ATGAA 1 cut(s) 142
TspGWI ACGGA 2 cut(s) 43, 409
TspRI CASTG 1 cut(s) 46
VpaK11BI GGWCC 2 cut(s) 60, 292
XagI CCTNNNNNAGG 1 cut(s) 29
XapI RAATTY 1 cut(s) 396
XceI RCATGY 1 cut(s) 51
XhoI CTCGAG 1 cut(s) 259
XspI CTAG 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.