MD10G1103500.v1.1

lob domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003322
Physical Location & Seq
Reverse (-)
16851002 .. 16852119
1118 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1103500.v1.1.491

Sequence Viewer

Length: 648 bp
ATGAGTTGCAATGGATGCCGAGTTCTAAGAAAGGGTTGTAGTGATAACTGCATCCTTAGGCAATGCCTACAATGGATGGAAGACCCCCAAGCTCAAGCCCACGCCACCGTTTTTGTGGCCAAGTTTTTTGGCCGCTCCGGCCTCTTCTCCTTCATCAACGCAGTCTCTGAGTCCCAACGACCCGCTTTGTTTCAGTCGTTAATATTTGAGGCGGTGGGGCGGACTATAAATCCTGTAAATGGGGCAACAGGGCTTCTTTTGAGTGGGAAGTGGGACGTGTGCCAGGCGGCGATAGAGACGGTGTTACGTGGTGGCGCGTTGGAGCCACTTCCCGAGTATATTCGTGACAACCATTATGTTCGTGAACATCAGGTTCGAGTAGAACAACGGCAATCCACCAAGAAGAAGAGGCCTAATTTTGCTGTCGATGATATTGAAGATGACGTGGCTAATTTCCAACCGCCCGATCTCAATCTATGCTTGACATCTGCAGCTGGGTTGACGTCGATGACGAAACGGAGGGATTGTAGAGCGGCAACGCCGTCAGTTGAAGACTCGGAAACGACAACGTTAGGGAGTTCTTCATCAGAGAACAATTGTACTGCTGGTAATTTAGAGAGAACAAAGCTTCTGAGGTTATTCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

216

Amino Acids

23.79

Weight (kDa)

7.53

Isoelectric Point (pI)

55.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 2 - 100 1.2e-23 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 506
AccBSI CCGCTC 2 cut(s) 135, 533
AccII CGCG 1 cut(s) 317
AciI CCGC 7 cut(s) 133, 183, 212, 220, 287, 461, 533
AclI AACGTT 1 cut(s) 569
AcoI YGGCCR 2 cut(s) 117, 130
AcyI GRCGYC 1 cut(s) 503
AfaI GTAC 1 cut(s) 601
AfiI CCNNNNNNNGG 1 cut(s) 239
AflIII ACRYGT 1 cut(s) 276
AgsI TTSAA 2 cut(s) 437, 551
AjiI CACGTC 2 cut(s) 277, 445
AjnI CCWGG 1 cut(s) 282
AloI GAACNNNNNNTCC 1 cut(s) 38
AluBI AGCT 3 cut(s) 92, 494, 628
AluI AGCT 3 cut(s) 92, 494, 628
Alw26I GTCTC 2 cut(s) 169, 290
AlwNI CAGNNNCTG 1 cut(s) 167
Ama87I CYCGRG 1 cut(s) 332
AoxI GGCC 4 cut(s) 117, 130, 139, 410
ApeKI GCWGC 1 cut(s) 491
AspLEI GCGC 1 cut(s) 317
AvaI CYCGRG 1 cut(s) 332
AxyI CCTNAGG 1 cut(s) 56
BalI TGGCCA 1 cut(s) 119
BbsI GAAGAC 2 cut(s) 87, 558
BbvI GCAGC 1 cut(s) 503
BccI CCATC 1 cut(s) 70
BceAI ACGGC 2 cut(s) 404, 526
BciT130I CCWGG 1 cut(s) 284
BcoDI GTCTC 2 cut(s) 169, 290
BfmI CTRYAG 1 cut(s) 489
BglI GCCNNNNNGGC 1 cut(s) 138
BisI GCNGC 4 cut(s) 133, 288, 492, 534
BlsI GCNGC 4 cut(s) 134, 289, 493, 535
Bme1390I CCNGG 1 cut(s) 284
BmeT110I CYCGRG 1 cut(s) 332
BmgBI CACGTC 2 cut(s) 277, 445
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 1 cut(s) 284
BmsI GCATC 2 cut(s) 5, 60
BpiI GAAGAC 2 cut(s) 87, 558
BpuEI CTTGAG 1 cut(s) 78
BsaAI YACGTR 1 cut(s) 308
BsaBI GATNNNNATC 1 cut(s) 471
BsaHI GRCGYC 1 cut(s) 503
BsaXI ACNNNNNCTCC 2 cut(s) 511, 541
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse21I CCTNAGG 1 cut(s) 56
Bse3DI GCAATG 2 cut(s) 16, 68
Bse8I GATNNNNATC 1 cut(s) 471
BseBI CCWGG 1 cut(s) 284
BseGI GGATG 3 cut(s) 20, 51, 81
BseJI GATNNNNATC 1 cut(s) 471
BseLI CCNNNNNNNGG 1 cut(s) 239
BseMI GCAATG 2 cut(s) 16, 68
BseMII CTCAG 2 cut(s) 159, 623
BseXI GCAGC 1 cut(s) 503
BseYI CCCAGC 1 cut(s) 494
Bsh1236I CGCG 1 cut(s) 317
BshFI GGCC 4 cut(s) 119, 132, 141, 412
BsiHKCI CYCGRG 1 cut(s) 332
BsiSI CCGG 1 cut(s) 138
BslFI GGGAC 2 cut(s) 157, 287
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 2 cut(s) 169, 290
BsmBI CGTCTC 1 cut(s) 290
BsmFI GGGAC 2 cut(s) 157, 287
BsnI GGCC 4 cut(s) 119, 132, 141, 412
BsoBI CYCGRG 1 cut(s) 332
Bsp143I GATC 1 cut(s) 466
BspACI CCGC 7 cut(s) 133, 183, 212, 220, 287, 461, 533
BspANI GGCC 4 cut(s) 119, 132, 141, 412
BspCNI CTCAG 2 cut(s) 160, 624
BspFNI CGCG 1 cut(s) 317
BspLI GGNNCC 1 cut(s) 324
BspMAI CTGCAG 1 cut(s) 493
BsrBI CCGCTC 2 cut(s) 135, 533
BsrDI GCAATG 2 cut(s) 16, 68
BssMI GATC 1 cut(s) 466
BssNI GRCGYC 1 cut(s) 503
Bst2UI CCWGG 1 cut(s) 284
Bst4CI ACNGT 2 cut(s) 109, 301
Bst6I CTCTTC 2 cut(s) 149, 401
BstACI GRCGYC 1 cut(s) 503
BstAPI GCANNNNNTGC 1 cut(s) 15
BstBAI YACGTR 1 cut(s) 308
BstDEI CTNAG 4 cut(s) 26, 56, 168, 632
BstF5I GGATG 3 cut(s) 20, 51, 81
BstFNI CGCG 1 cut(s) 317
BstHHI GCGC 1 cut(s) 317
BstKTI GATC 1 cut(s) 469
BstMAI GTCTC 2 cut(s) 169, 290
BstMBI GATC 1 cut(s) 466
BstMWI GCNNNNNNNGC 2 cut(s) 15, 138
BstNI CCWGG 1 cut(s) 284
BstSCI CCNGG 1 cut(s) 282
BstSFI CTRYAG 1 cut(s) 489
BstUI CGCG 1 cut(s) 317
BstV1I GCAGC 1 cut(s) 503
BstV2I GAAGAC 2 cut(s) 87, 558
Bsu36I CCTNAGG 1 cut(s) 56
BsuRI GGCC 4 cut(s) 119, 132, 141, 412
BtrI CACGTC 2 cut(s) 277, 445
BtsCI GGATG 3 cut(s) 20, 51, 81
CaiI CAGNNNCTG 1 cut(s) 167
CfoI GCGC 1 cut(s) 317
Csp6I GTAC 1 cut(s) 600
CspCI CAANNNNNGTGG 2 cut(s) 94, 129
CviQI GTAC 1 cut(s) 600
DdeI CTNAG 4 cut(s) 26, 56, 168, 632
DpnI GATC 1 cut(s) 468
DpnII GATC 1 cut(s) 466
EaeI YGGCCR 2 cut(s) 117, 130
Eam1104I CTCTTC 2 cut(s) 149, 401
EarI CTCTTC 2 cut(s) 149, 401
EciI GGCGGA 1 cut(s) 235
Eco147I AGGCCT 1 cut(s) 412
Eco81I CCTNAGG 1 cut(s) 56
Eco88I CYCGRG 1 cut(s) 332
EcoRII CCWGG 1 cut(s) 282
Esp3I CGTCTC 1 cut(s) 290
FaiI YATR 4 cut(s) 227, 339, 357, 478
FaqI GGGAC 2 cut(s) 157, 287
FauI CCCGC 1 cut(s) 190
Fnu4HI GCNGC 4 cut(s) 133, 288, 492, 534
FokI GGATG 3 cut(s) 27, 38, 88
Fsp4HI GCNGC 4 cut(s) 133, 288, 492, 534
GlaI GCGC 1 cut(s) 316
GluI GCNGC 4 cut(s) 133, 288, 492, 534
GsaI CCCAGC 1 cut(s) 498
HaeIII GGCC 4 cut(s) 119, 132, 141, 412
HapII CCGG 1 cut(s) 138
HhaI GCGC 1 cut(s) 317
Hin1I GRCGYC 1 cut(s) 503
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HincII GTYRAC 1 cut(s) 501
HindII GTYRAC 1 cut(s) 501
HindIII AAGCTT 1 cut(s) 626
HinfI GANTC 2 cut(s) 170, 554
HpaII CCGG 1 cut(s) 138
Hpy166II GTNNAC 2 cut(s) 365, 501
Hpy188I TCNGA 4 cut(s) 169, 559, 589, 633
Hpy188III TCNNGA 3 cut(s) 332, 344, 362
Hpy8I GTNNAC 2 cut(s) 365, 501
Hpy99I CGWCG 1 cut(s) 508
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 2 cut(s) 109, 301
HpyCH4IV ACGT 5 cut(s) 276, 307, 444, 503, 569
HpyCH4V TGCA 3 cut(s) 9, 51, 491
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 138
HpyF3I CTNAG 4 cut(s) 26, 56, 168, 632
HpySE526I ACGT 5 cut(s) 276, 307, 444, 503, 569
Hsp92I GRCGYC 1 cut(s) 503
HspAI GCGC 1 cut(s) 315
Kzo9I GATC 1 cut(s) 466
LmnI GCTCC 2 cut(s) 140, 322
LpnPI CCDG 8 cut(s) 151, 234, 246, 269, 296, 356, 480, 591
Lsp1109I GCAGC 1 cut(s) 503
LweI GCATC 2 cut(s) 5, 60
MaeII ACGT 5 cut(s) 276, 307, 444, 503, 569
MaeIII GTNAC 2 cut(s) 303, 344
MalI GATC 1 cut(s) 468
MbiI CCGCTC 2 cut(s) 135, 533
MboI GATC 1 cut(s) 466
MboII GAAGA 7 cut(s) 92, 136, 415, 418, 449, 563, 573
MfeI CAATTG 1 cut(s) 595
MlsI TGGCCA 1 cut(s) 119
MluCI AATT 4 cut(s) 415, 451, 595, 610
MluNI TGGCCA 1 cut(s) 119
MlyI GAGTC 2 cut(s) 179, 548
MmeI TCCRAC 2 cut(s) 300, 481
MnlI CCTC 5 cut(s) 152, 202, 402, 513, 627
Mox20I TGGCCA 1 cut(s) 119
MscI TGGCCA 1 cut(s) 119
MseI TTAA 1 cut(s) 200
Msp20I TGGCCA 1 cut(s) 119
MspA1I CMGCKG 1 cut(s) 494
MspI CCGG 1 cut(s) 138
MspR9I CCNGG 1 cut(s) 284
MunI CAATTG 1 cut(s) 595
MvaI CCWGG 1 cut(s) 284
MvnI CGCG 1 cut(s) 317
MwoI GCNNNNNNNGC 2 cut(s) 15, 138
NdeII GATC 1 cut(s) 466
NlaIV GGNNCC 1 cut(s) 324
NmeAIII GCCGAG 1 cut(s) 44
NmuCI GTSAC 1 cut(s) 344
PceI AGGCCT 1 cut(s) 412
PcsI WCGNNNNNNNCGW 1 cut(s) 509
PkrI GCNGC 4 cut(s) 134, 289, 493, 535
PleI GAGTC 2 cut(s) 178, 548
PpsI GAGTC 2 cut(s) 178, 548
Ppu21I YACGTR 1 cut(s) 308
Psp1406I AACGTT 1 cut(s) 569
Psp6I CCWGG 1 cut(s) 282
PspFI CCCAGC 1 cut(s) 494
PspGI CCWGG 1 cut(s) 282
PspN4I GGNNCC 1 cut(s) 324
PstI CTGCAG 1 cut(s) 493
PstNI CAGNNNCTG 1 cut(s) 167
PvuII CAGCTG 1 cut(s) 494
RsaI GTAC 1 cut(s) 601
RsaNI GTAC 1 cut(s) 600
SaqAI TTAA 1 cut(s) 200
SatI GCNGC 4 cut(s) 133, 288, 492, 534
Sau3AI GATC 1 cut(s) 466
SchI GAGTC 2 cut(s) 179, 548
ScrFI CCNGG 1 cut(s) 284
SfaNI GCATC 2 cut(s) 5, 60
SfcI CTRYAG 1 cut(s) 489
SfiI GGCCNNNNNGGCC 1 cut(s) 138
SmlI CTYRAG 1 cut(s) 93
SmoI CTYRAG 1 cut(s) 93
Sse9I AATT 4 cut(s) 415, 451, 595, 610
SseBI AGGCCT 1 cut(s) 412
SsiI CCGC 7 cut(s) 133, 183, 212, 220, 287, 461, 533
SspI AATATT 1 cut(s) 204
StuI AGGCCT 1 cut(s) 412
StyD4I CCNGG 1 cut(s) 282
TaaI ACNGT 2 cut(s) 109, 301
TaiI ACGT 5 cut(s) 279, 310, 447, 506, 572
TaqI TCGA 3 cut(s) 376, 426, 506
TasI AATT 4 cut(s) 415, 451, 595, 610
TatI WGTACW 1 cut(s) 599
TauI GCSGC 3 cut(s) 135, 290, 536
Tru1I TTAA 1 cut(s) 200
Tru9I TTAA 1 cut(s) 200
TseFI GTSAC 1 cut(s) 344
TseI GCWGC 1 cut(s) 491
Tsp45I GTSAC 1 cut(s) 344
TspDTI ATGAA 3 cut(s) 142, 573, 631
TspGWI ACGGA 1 cut(s) 532
XcmI CCANNNNNNNNNTGG 1 cut(s) 112
ZraI GACGTC 1 cut(s) 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.