FvH4_2g05530

At4g06744-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004065
Physical Location & Seq
Forward (+)
4567976 .. 4570257
2282 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05530.t1

Sequence Viewer

Length: 1515 bp
ATGGGTACCAAACATTCTTCCTCTGCTTCAGTTTTGCTTTCACTACTTCTGCTACATTTGTGTCTGCAACATGATGTAGTTGAATGTGCTGAAAGAGAAGCTTTGGAAATAATCATCGGTGGTGGTGGCGGCAGCTACAGCCCCCCTGCTCCCTCCCCCGAATACGCTCCCTCCCCCGAATACGCTCCCTCCCCTGAATACGTAGACTGCCCTCCCCCTCCCCCTCCTCCTTGCCCACCGCCACCATCCCCGTCACCGCCACCACCGTCACCTCCACCACCACCACCTCCGTCCCCACCACCACCGCCGCCGCCACGTCCACCACCACTACCTCCGTCCCCACCACCACCACTACCGCCACGACCACCCGCTCCGGCACCACCGAACGAGAGACTTGCAATTGCCCTCAGGGTAATTAAAAGGTTCCAAAAGCTCATCGGGGTGGATCCAGATAACATCACAGGCACATGGAATGGCGATAATCCTTGCAAATTCACAGGCTTTATCTGTGGTGTTGTCCCTGTCTATAACCAAAACGCAGTCGCCGGAGTTAACTTCAACGGCAGAAAGTTGGCTGGCAAATGGGGCGGCAAAGAAACAAAGCTACCCCTTGACGGTTTCCTAGACCAGCTACCAGACCTAGTGTTTTTCCATGCCAACTCCAACAATTTCACAGGCCAAGTTCCGATTGGTACATCCAGACTTCCATACTTCTACGAGCTCGACCTCAGTAACAACAAGCTAGATGGAGGGTTTCCCTATGAAGTTCTAAGTGCCCCAAACTTGACATTCTTGGATCTCCGGTTCAATAATTACCAAGGTTCAGTTCCAAGCCAAGTGTTCCTCTACAAAGACATTGATGTAGTCTTCCTCAACAACAACAACTTCAATGAGAAGATTCCTGATGCTATTGGCTCCTCAACAGCACATTATCTCACTTTTGCCAACAACAAGTTCTGGGGTCCGATCCCAAGAACCATCGGCAAAGCAAGTAAAACTCTTTTTGAGGTCCTCTTCCTCAATAACAGACTCTCAGGGTGTTTGCCAATGGAAATCGGAAAACTTAACCAGGCAACGTTGTTCGATGTGAGCTACAACCAGTTAACAGGGCCAATTCCGCAGTCGTTTGCTTGCTTGACCAAGATGGAGATTCTGGTCTTGGACAACAACCAGTTCTATGGAGCAGTGCCTGAGTTGGTATGCAAGCTGCCCAGCTTGATGAACTTGTCATTGGCAAACAACTACTTCACCCAAGTCGGACCGGCGTGCAGGAAACTGATCAAGAAAGGGTTTCTCCATGTCGACAACAACTGTATCTTGGACCTCAAATACCAAAGACCAAAGAGTGAATGTGCTTCGTTCTTCTCTAAGCCTAGATACTGTCCCAACGAGCGGTCCTTGCTTTACATTCCTTGTAAAGGGAAATATTCAGGTGAGAATTCGAAGAAGCTTGAACATGGAGGACAAGCTTCTGCCCCATTAAGTTATGGTACTCTTAGACCACATAATCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2

Protein Analysis

505

Amino Acids

54.96

Weight (kDa)

8.42

Isoelectric Point (pI)

56.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_ComC PF24141 278 - 390 2.6e-06 EGF-like domain-containing protein ComC LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 5
AccB1I GGYRCC 2 cut(s) 5, 376
AccBSI CCGCTC 2 cut(s) 371, 1393
AccI GTMKAC 2 cut(s) 204, 1302
AclI AACGTT 1 cut(s) 1076
AclWI GGATC 4 cut(s) 440, 453, 804, 961
AcsI RAATTY 2 cut(s) 491, 1438
AcuI CTGAAG 1 cut(s) 12
AfaI GTAC 3 cut(s) 7, 694, 1492
AfiI CCNNNNNNNGG 3 cut(s) 614, 1392, 1418
AgsI TTSAA 5 cut(s) 83, 559, 808, 889, 1454
AjiI CACGTC 1 cut(s) 317
AjnI CCWGG 1 cut(s) 1068
AjuI GAANNNNNNNTTGG 6 cut(s) 810, 842, 938, 970, 1214, 1246
AloI GAACNNNNNNTCC 2 cut(s) 788, 820
Alw21I GWGCWC 1 cut(s) 723
Alw26I GTCTC 1 cut(s) 385
AlwI GGATC 4 cut(s) 440, 453, 804, 961
AlwNI CAGNNNCTG 1 cut(s) 1190
AoxI GGCC 2 cut(s) 676, 1109
ApeKI GCWGC 2 cut(s) 132, 1207
ApoI RAATTY 2 cut(s) 491, 1438
Asp718I GGTACC 1 cut(s) 5
AspS9I GGNCC 6 cut(s) 962, 1009, 1109, 1259, 1321, 1395
AsuHPI GGTGA 4 cut(s) 246, 261, 1240, 1445
AsuII TTCGAA 1 cut(s) 1442
AvaII GGWCC 5 cut(s) 962, 1009, 1259, 1321, 1395
AxyI CCTNAGG 1 cut(s) 407
BaeGI GKGCMC 1 cut(s) 778
BaeI ACNNNNGTAYC 2 cut(s) 1297, 1330
BamHI GGATCC 1 cut(s) 445
BanI GGYRCC 2 cut(s) 5, 376
BanII GRGCYC 1 cut(s) 723
BbsI GAAGAC 1 cut(s) 859
Bbv12I GWGCWC 1 cut(s) 723
BbvI GCAGC 2 cut(s) 144, 1194
BccI CCATC 4 cut(s) 253, 740, 986, 1138
BceAI ACGGC 1 cut(s) 577
BcgI CGANNNNNNTGC 2 cut(s) 377, 411
BciT130I CCWGG 1 cut(s) 1070
BclI TGATCA 1 cut(s) 1278
BcoDI GTCTC 1 cut(s) 385
BfaI CTAG 4 cut(s) 623, 641, 743, 1374
BfmI CTRYAG 1 cut(s) 136
BisI GCNGC 6 cut(s) 130, 133, 308, 311, 589, 1208
BlsI GCNGC 6 cut(s) 131, 134, 309, 312, 590, 1209
Bme1390I CCNGG 1 cut(s) 1070
Bme18I GGWCC 5 cut(s) 962, 1009, 1259, 1321, 1395
BmgBI CACGTC 1 cut(s) 317
BmgT120I GGNCC 6 cut(s) 962, 1009, 1109, 1259, 1321, 1395
BmiI GGNNCC 6 cut(s) 7, 378, 425, 447, 916, 963
BmrFI CCNGG 1 cut(s) 1070
BmsI GCATC 1 cut(s) 895
BpiI GAAGAC 1 cut(s) 859
Bpu14I TTCGAA 1 cut(s) 1442
BsaAI YACGTR 1 cut(s) 202
BsaJI CCNNGG 1 cut(s) 817
BsaWI WCCGGW 1 cut(s) 801
BsaXI ACNNNNNCTCC 4 cut(s) 155, 173, 185, 203
Bsc4I CCNNNNNNNGG 3 cut(s) 614, 1392, 1418
Bse118I RCCGGY 1 cut(s) 1261
Bse1I ACTGG 2 cut(s) 1099, 1171
Bse21I CCTNAGG 1 cut(s) 407
BseBI CCWGG 1 cut(s) 1070
BseDI CCNNGG 1 cut(s) 817
BseGI GGATG 2 cut(s) 245, 695
BseLI CCNNNNNNNGG 3 cut(s) 614, 1392, 1418
BseMII CTCAG 4 cut(s) 421, 742, 1047, 1182
BseNI ACTGG 2 cut(s) 1099, 1171
BseRI GAGGAG 2 cut(s) 216, 907
BseSI GKGCMC 1 cut(s) 778
BseXI GCAGC 2 cut(s) 144, 1194
BseYI CCCAGC 1 cut(s) 1211
BsgI GTGCAG 1 cut(s) 1288
BshFI GGCC 2 cut(s) 678, 1111
BshNI GGYRCC 2 cut(s) 5, 376
BsiHKAI GWGCWC 1 cut(s) 723
BsiSI CCGG 4 cut(s) 374, 546, 802, 1262
BslFI GGGAC 4 cut(s) 277, 322, 503, 1368
BslI CCNNNNNNNGG 3 cut(s) 614, 1392, 1418
BsmAI GTCTC 1 cut(s) 385
BsmFI GGGAC 4 cut(s) 277, 322, 503, 1368
BsnI GGCC 2 cut(s) 678, 1111
Bsp119I TTCGAA 1 cut(s) 1442
Bsp1286I GDGCHC 2 cut(s) 723, 778
Bsp143I GATC 4 cut(s) 445, 796, 966, 1278
BspANI GGCC 2 cut(s) 678, 1111
BspCNI CTCAG 4 cut(s) 420, 741, 1046, 1183
BspLI GGNNCC 6 cut(s) 7, 378, 425, 447, 916, 963
BspPI GGATC 4 cut(s) 440, 453, 804, 961
BspT104I TTCGAA 1 cut(s) 1442
BspT107I GGYRCC 2 cut(s) 5, 376
BsrBI CCGCTC 2 cut(s) 371, 1393
BsrFI RCCGGY 1 cut(s) 1261
BsrI ACTGG 2 cut(s) 1099, 1171
BssAI RCCGGY 1 cut(s) 1261
BssECI CCNNGG 1 cut(s) 817
BssMI GATC 4 cut(s) 445, 796, 966, 1278
BssT1I CCWWGG 1 cut(s) 817
Bst2UI CCWGG 1 cut(s) 1070
Bst4CI ACNGT 4 cut(s) 267, 617, 1313, 1382
Bst6I CTCTTC 1 cut(s) 1019
BstBAI YACGTR 1 cut(s) 202
BstBI TTCGAA 1 cut(s) 1442
BstC8I GCNNGC 4 cut(s) 577, 1132, 1205, 1267
BstDEI CTNAG 7 cut(s) 407, 728, 770, 1033, 1191, 1368, 1496
BstENI CCTNNNNNAGG 1 cut(s) 1416
BstF5I GGATG 2 cut(s) 245, 695
BstKTI GATC 4 cut(s) 448, 799, 969, 1281
BstMAI GTCTC 1 cut(s) 385
BstMBI GATC 4 cut(s) 445, 796, 966, 1278
BstMWI GCNNNNNNNGC 4 cut(s) 138, 585, 1117, 1399
BstNI CCWGG 1 cut(s) 1070
BstSCI CCNGG 1 cut(s) 1068
BstSFI CTRYAG 1 cut(s) 136
BstSLI GKGCMC 1 cut(s) 778
BstSNI TACGTA 1 cut(s) 202
BstV1I GCAGC 2 cut(s) 144, 1194
BstV2I GAAGAC 1 cut(s) 859
BstX2I RGATCY 2 cut(s) 445, 796
BstXI CCANNNNNNTGG 1 cut(s) 1178
BstYI RGATCY 2 cut(s) 445, 796
Bsu36I CCTNAGG 1 cut(s) 407
BsuRI GGCC 2 cut(s) 678, 1111
BtrI CACGTC 1 cut(s) 317
BtsCI GGATG 2 cut(s) 245, 695
BtsI GCAGTG 1 cut(s) 1191
BtsIMutI CAGTG 1 cut(s) 1191
Cac8I GCNNGC 4 cut(s) 577, 1132, 1205, 1267
CaiI CAGNNNCTG 1 cut(s) 1190
Cfr10I RCCGGY 1 cut(s) 1261
Cfr13I GGNCC 6 cut(s) 962, 1009, 1109, 1259, 1321, 1395
CpoI CGGWCCG 1 cut(s) 1259
Csp6I GTAC 3 cut(s) 6, 693, 1491
CspI CGGWCCG 1 cut(s) 1259
CviAII CATG 5 cut(s) 71, 468, 653, 1298, 1457
CviQI GTAC 3 cut(s) 6, 693, 1491
DdeI CTNAG 7 cut(s) 407, 728, 770, 1033, 1191, 1368, 1496
DpnI GATC 4 cut(s) 447, 798, 968, 1280
DpnII GATC 4 cut(s) 445, 796, 966, 1278
Eam1104I CTCTTC 1 cut(s) 1019
EarI CTCTTC 1 cut(s) 1019
Ecl136II GAGCTC 1 cut(s) 721
Eco105I TACGTA 1 cut(s) 202
Eco130I CCWWGG 1 cut(s) 817
Eco24I GRGCYC 1 cut(s) 723
Eco47I GGWCC 5 cut(s) 962, 1009, 1259, 1321, 1395
Eco53kI GAGCTC 1 cut(s) 721
Eco57I CTGAAG 1 cut(s) 12
Eco81I CCTNAGG 1 cut(s) 407
EcoICRI GAGCTC 1 cut(s) 721
EcoNI CCTNNNNNAGG 1 cut(s) 1416
EcoO109I RGGNCCY 1 cut(s) 1009
EcoRI GAATTC 1 cut(s) 1438
EcoRII CCWGG 1 cut(s) 1068
EcoT14I CCWWGG 1 cut(s) 817
EcoT38I GRGCYC 1 cut(s) 723
ErhI CCWWGG 1 cut(s) 817
FaeI CATG 5 cut(s) 74, 471, 656, 1301, 1460
FalI AAGNNNNNCTT 2 cut(s) 85, 117
FaqI GGGAC 4 cut(s) 277, 322, 503, 1368
FatI CATG 5 cut(s) 70, 467, 652, 1297, 1456
FauI CCCGC 1 cut(s) 376
FbaI TGATCA 1 cut(s) 1278
FblI GTMKAC 2 cut(s) 204, 1302
Fnu4HI GCNGC 6 cut(s) 130, 133, 308, 311, 589, 1208
FokI GGATG 2 cut(s) 232, 682
FriOI GRGCYC 1 cut(s) 723
Fsp4HI GCNGC 6 cut(s) 130, 133, 308, 311, 589, 1208
FspBI CTAG 4 cut(s) 623, 641, 743, 1374
GluI GCNGC 6 cut(s) 130, 133, 308, 311, 589, 1208
GsaI CCCAGC 1 cut(s) 1215
HaeIII GGCC 2 cut(s) 678, 1111
HapII CCGG 4 cut(s) 374, 546, 802, 1262
Hin1II CATG 5 cut(s) 74, 471, 656, 1301, 1460
HincII GTYRAC 3 cut(s) 553, 1104, 1303
HindII GTYRAC 3 cut(s) 553, 1104, 1303
HindIII AAGCTT 3 cut(s) 99, 1448, 1467
HinfI GANTC 3 cut(s) 898, 1029, 1150
HpaI GTTAAC 2 cut(s) 553, 1104
HpaII CCGG 4 cut(s) 374, 546, 802, 1262
HphI GGTGA 4 cut(s) 246, 261, 1240, 1445
Hpy166II GTNNAC 5 cut(s) 205, 320, 553, 1104, 1303
Hpy188I TCNGA 4 cut(s) 687, 966, 1058, 1259
Hpy188III TCNNGA 4 cut(s) 449, 699, 902, 1282
Hpy8I GTNNAC 5 cut(s) 205, 320, 553, 1104, 1303
HpyCH4III ACNGT 4 cut(s) 267, 617, 1313, 1382
HpyCH4IV ACGT 3 cut(s) 201, 316, 1076
HpyCH4V TGCA 5 cut(s) 67, 398, 489, 1203, 1269
HpyF10VI GCNNNNNNNGC 4 cut(s) 138, 585, 1117, 1399
HpyF3I CTNAG 7 cut(s) 407, 728, 770, 1033, 1191, 1368, 1496
HpySE526I ACGT 3 cut(s) 201, 316, 1076
Hsp92II CATG 5 cut(s) 74, 471, 656, 1301, 1460
KpnI GGTACC 1 cut(s) 9
Ksp22I TGATCA 1 cut(s) 1278
KspAI GTTAAC 2 cut(s) 553, 1104
Kzo9I GATC 4 cut(s) 445, 796, 966, 1278
LmnI GCTCC 6 cut(s) 154, 172, 190, 376, 920, 1181
Lsp1109I GCAGC 2 cut(s) 144, 1194
LweI GCATC 1 cut(s) 895
MaeI CTAG 4 cut(s) 623, 641, 743, 1374
MaeII ACGT 3 cut(s) 201, 316, 1076
MaeIII GTNAC 3 cut(s) 252, 267, 731
MalI GATC 4 cut(s) 447, 798, 968, 1280
MbiI CCGCTC 2 cut(s) 371, 1393
MboI GATC 4 cut(s) 445, 796, 966, 1278
MboII GAAGA 6 cut(s) 9, 859, 907, 1006, 1354, 1456
MfeI CAATTG 1 cut(s) 399
MflI RGATCY 2 cut(s) 445, 796
MhlI GDGCHC 2 cut(s) 723, 778
MluCI AATT 7 cut(s) 399, 414, 491, 667, 811, 1113, 1438
MlyI GAGTC 1 cut(s) 1023
MmeI TCCRAC 2 cut(s) 687, 1237
MseI TTAA 5 cut(s) 417, 552, 1065, 1103, 1481
MslI CAYNNNNRTG 1 cut(s) 440
MspI CCGG 4 cut(s) 374, 546, 802, 1262
MspR9I CCNGG 1 cut(s) 1070
MunI CAATTG 1 cut(s) 399
MvaI CCWGG 1 cut(s) 1070
MwoI GCNNNNNNNGC 4 cut(s) 138, 585, 1117, 1399
NdeII GATC 4 cut(s) 445, 796, 966, 1278
NlaIII CATG 5 cut(s) 74, 471, 656, 1301, 1460
NlaIV GGNNCC 6 cut(s) 7, 378, 425, 447, 916, 963
NmuCI GTSAC 2 cut(s) 252, 267
NspV TTCGAA 1 cut(s) 1442
PfeI GAWTC 2 cut(s) 898, 1150
PkrI GCNGC 6 cut(s) 131, 134, 309, 312, 590, 1209
PleI GAGTC 1 cut(s) 1023
PpsI GAGTC 1 cut(s) 1023
Ppu21I YACGTR 1 cut(s) 202
PpuMI RGGWCCY 1 cut(s) 1009
Psp124BI GAGCTC 1 cut(s) 723
Psp1406I AACGTT 1 cut(s) 1076
Psp5II RGGWCCY 1 cut(s) 1009
Psp6I CCWGG 1 cut(s) 1068
PspFI CCCAGC 1 cut(s) 1211
PspGI CCWGG 1 cut(s) 1068
PspN4I GGNNCC 6 cut(s) 7, 378, 425, 447, 916, 963
PspPI GGNCC 6 cut(s) 962, 1009, 1109, 1259, 1321, 1395
PspPPI RGGWCCY 1 cut(s) 1009
PstNI CAGNNNCTG 1 cut(s) 1190
PsuI RGATCY 2 cut(s) 445, 796
RsaI GTAC 3 cut(s) 7, 694, 1492
RsaNI GTAC 3 cut(s) 6, 693, 1491
RseI CAYNNNNRTG 1 cut(s) 440
Rsr2I CGGWCCG 1 cut(s) 1259
RsrII CGGWCCG 1 cut(s) 1259
SacI GAGCTC 1 cut(s) 723
SalI GTCGAC 1 cut(s) 1301
SaqAI TTAA 5 cut(s) 417, 552, 1065, 1103, 1481
SatI GCNGC 6 cut(s) 130, 133, 308, 311, 589, 1208
Sau3AI GATC 4 cut(s) 445, 796, 966, 1278
Sau96I GGNCC 6 cut(s) 962, 1009, 1109, 1259, 1321, 1395
SchI GAGTC 1 cut(s) 1023
ScrFI CCNGG 1 cut(s) 1070
SduI GDGCHC 2 cut(s) 723, 778
SfaNI GCATC 1 cut(s) 895
SfcI CTRYAG 1 cut(s) 136
SfuI TTCGAA 1 cut(s) 1442
SinI GGWCC 5 cut(s) 962, 1009, 1259, 1321, 1395
SmiMI CAYNNNNRTG 1 cut(s) 440
SnaBI TACGTA 1 cut(s) 202
Sse9I AATT 7 cut(s) 399, 414, 491, 667, 811, 1113, 1438
SspI AATATT 1 cut(s) 1427
SspMI CTAG 4 cut(s) 623, 641, 743, 1374
SstI GAGCTC 1 cut(s) 723
StyD4I CCNGG 1 cut(s) 1068
StyI CCWWGG 1 cut(s) 817
TaaI ACNGT 4 cut(s) 267, 617, 1313, 1382
TaiI ACGT 3 cut(s) 204, 319, 1079
TaqI TCGA 4 cut(s) 723, 1083, 1302, 1442
TasI AATT 7 cut(s) 399, 414, 491, 667, 811, 1113, 1438
TauI GCSGC 4 cut(s) 132, 310, 313, 591
TfiI GAWTC 2 cut(s) 898, 1150
Tru1I TTAA 5 cut(s) 417, 552, 1065, 1103, 1481
Tru9I TTAA 5 cut(s) 417, 552, 1065, 1103, 1481
TscAI CASTG 1 cut(s) 1191
TseFI GTSAC 2 cut(s) 252, 267
TseI GCWGC 2 cut(s) 132, 1207
Tsp45I GTSAC 2 cut(s) 252, 267
TspDTI ATGAA 2 cut(s) 777, 1235
TspGWI ACGGA 2 cut(s) 279, 324
TspRI CASTG 1 cut(s) 1191
VpaK11BI GGWCC 5 cut(s) 962, 1009, 1259, 1321, 1395
XagI CCTNNNNNAGG 1 cut(s) 1416
XapI RAATTY 2 cut(s) 491, 1438
XcmI CCANNNNNNNNNTGG 1 cut(s) 686
XmiI GTMKAC 2 cut(s) 204, 1302
XspI CTAG 4 cut(s) 623, 641, 743, 1374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.