Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0004065

Physical Location & Seq

Genomic Coordinates Forward (+)

10993480 .. 10995769

Gene Structure 2290 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G006880.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1521 bp

ATGGGTACCAACAATTCTTTCTTTGCTTCAGTTTTGCTTTCACTACTTCTGCTACATTTGTGTTTGCAACATGATGTAGTTGAAGGTGCTGAAAGAGAAGCCTTGGAAATAATCATTGGTGGTGGCGGCAGCTACAGCCCTCCTCCTGCTCCCTCCCCCGAATACGAAGAATGCCCTCCCCCTCCGCCTCCTCCTTGCCCGCCGCCACCATTCCCGCCACCGCCACCTCCATCACCACCGCCGCCGTCACCTCCTCCACCACCATCACCACCACCGCCGTCACCTCCTCCACCACCATCACCACCACCGCCGTCACCTCCTCCACCACCATCGCCGCCACCTCCACCGCCACCATCACCTCCACCACGACCGCGACCGCGACCGCGACCACCACCCCCTCCGAACACCAGACTTGCAAATGCCCTCCGAGTAATTCGTAAGTTCAAAAAGCTAATCGTCGAAGACCCATACAAAATCACAAAAACATGGAACGGCAATGATCCTTGCAAATTCAAGGGCTTCACCTGTGCAATGTTCCCTGACGATAAACAAATGGCAGTCGCCGGAGTTGACTTCAATGGCAAACGGTTGGCCGGTAATAAAACAGCTAAGCAACCCCTTCCCCTTGACGGTTTCCTAGACCAGTTACCAGAGTTGGTGTTTTTCCATGCCAACTCCAACAATTTCACAGGCTCAGTCCCGAAAGACACATCCAAAATTAAATACTTCTACGAGCTTGACCTCAGCAACAACAAGCTTGATGGGGGTTTCCCACATGAAGTTCTAAGTGCCAAAAATTTGACATTCTTAGATCTCCGGTTCAACAATTTCCATGGGTCAGTTCCACCCGAAGTGTTCATCCTGGACGTTGATGTACTCTTCCTCAACAACAACCATTTCATTGAGCAGATTCCTGATGCTCTTGGCTCCTCACCAGCACATTATCTCACTTTTGCCAACAACAAGTTCTGGGGTCCAATCCCAAATAGCATTGGCAAAGCATCAAAAACGCTTTATGAGGTCCTCTTCCTCAACAACCAACTCTCAGGGTGTTTGCCATGGGAAATCGGCAAACTTAACCAGGCAACCTTGTTCGATGTGAGCACCAACTACTTAACAGGCCCAATACCTCATTCCTTTGCTTGCTTGGCCAAGATGGAGATTCTGGTCTTGGCCAACAACCAGTTCTATGGAACAGTGCCTGAGTTGGTATGCAAGCTGCCCGTCTTGAGGAACTTGTCGTTAGCAAACAACTATTTTACCCAAGTCGGACCGGAGTGCAGAAAACTGATCAATAAAGGGTTTCTCAACGTCAAGAACAATTGTATCTTGGACCTTAAAGATCAAAGATCAAAGAGTGATTGTGCTTGGTTCTTCTCTAAGGCCAGACACTGTCCCAACGAGCGGTCCATGCTTTACATTCCTTGTCAAACGCACTATTCAAATGAGAGGAAGCCTGAACATCAAGGACAAGCTTCTGCCCCATTAAGTTATGGTACTCTTAAACCGCATAATCCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2

Protein Analysis

506

Amino Acids

55.69

Weight (kDa)

8.57

Isoelectric Point (pI)

62.71

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49750 AT3G19320
fragaria_vesca	FvH4_2g05530
malus_domestica	MD00G1014300.v1.1 MD05G1086700.v1.1
prunus_persica	Prupe.8G131400_v2.0.a1 Prupe.8G131500_v2.0.a1
pyrus_communis	pycom05g08610 pycom10g08130
rosa_chinensis	RchiOBHm_Chr4g0387761 RchiOBHm_Chr6g0255781 RchiOBHm_Chr6g0255841
rosa_laevigata	RLG00000014812 RLG00000014813
rosa_multiflora	Rmu_sc0001163.1_g000027 Rmu_sc0001163.1_g000029 Rmu_sc0004935.1_g000023
rosa_roxburghii	Rroxscaffold_7G00209620 Rroxscaffold_7G00209650
rosa_rugosa	Rorug05G0561500 Rorug05G0561700
rosa_samantha	Rh1AG424200 Rh4BG013200 Rh6AG079500 Rh6AG079800 Rh6BG072600 Rh6BG072700 Rh6CG070700 Rh6CG070800 Rh6DG068000 Rh6DG068100
rosa_wichuraiana	Rw6G006850 Rw6G006880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	5
AccB1I	GGYRCC	1 cut(s)	5
AccBSI	CCGCTC	1 cut(s)	1405
AccII	CGCG	3 cut(s)	373, 379, 385
AclWI	GGATC	1 cut(s)	494
AcoI	YGGCCR	3 cut(s)	591, 1149, 1173
AcsI	RAATTY	2 cut(s)	509, 796
AcuI	CTGAAG	1 cut(s)	12
AfaI	GTAC	3 cut(s)	7, 876, 1498
AfiI	CCNNNNNNNGG	3 cut(s)	629, 1230, 1404
AgsI	TTSAA	6 cut(s)	83, 445, 514, 577, 823, 1443
AjnI	CCWGG	2 cut(s)	861, 1080
AjuI	GAANNNNNNNTTGG	9 cut(s)	34, 99, 131, 950, 982, 1117, 1149, 1169, 1201
AluBI	AGCT	7 cut(s)	132, 451, 608, 736, 757, 1219, 1475
AluI	AGCT	7 cut(s)	132, 451, 608, 736, 757, 1219, 1475
Alw21I	GWGCWC	1 cut(s)	1106
AlwI	GGATC	1 cut(s)	494
AlwNI	CAGNNNCTG	2 cut(s)	1202, 1392
AoxI	GGCC	5 cut(s)	591, 1120, 1149, 1173, 1383
ApeKI	GCWGC	2 cut(s)	129, 1219
ApoI	RAATTY	2 cut(s)	509, 796
Asp718I	GGTACC	1 cut(s)	5
AspS9I	GGNCC	6 cut(s)	974, 1021, 1121, 1271, 1333, 1407
AsuHPI	GGTGA	9 cut(s)	225, 240, 258, 273, 291, 306, 348, 514, 924
AvaII	GGWCC	5 cut(s)	974, 1021, 1271, 1333, 1407
BaeI	ACNNNNGTAYC	2 cut(s)	1309, 1342
BalI	TGGCCA	2 cut(s)	1151, 1175
BanI	GGYRCC	1 cut(s)	5
BbsI	GAAGAC	1 cut(s)	468
Bbv12I	GWGCWC	1 cut(s)	1106
BbvCI	CCTCAGC	1 cut(s)	743
BbvI	GCAGC	2 cut(s)	141, 1206
BccI	CCATC	7 cut(s)	238, 271, 304, 337, 361, 755, 1150
BceAI	ACGGC	4 cut(s)	229, 262, 295, 508
BciT130I	CCWGG	2 cut(s)	863, 1082
BclI	TGATCA	1 cut(s)	1290
BfaI	CTAG	1 cut(s)	638
BfmI	CTRYAG	1 cut(s)	133
BglII	AGATCT	1 cut(s)	811
BisI	GCNGC	6 cut(s)	127, 130, 203, 242, 335, 1220
BlpI	GCTNAGC	1 cut(s)	609
BlsI	GCNGC	6 cut(s)	128, 131, 204, 243, 336, 1221
Bme1390I	CCNGG	2 cut(s)	863, 1082
Bme18I	GGWCC	5 cut(s)	974, 1021, 1271, 1333, 1407
BmgT120I	GGNCC	6 cut(s)	974, 1021, 1121, 1271, 1333, 1407
BmiI	GGNNCC	3 cut(s)	7, 928, 975
BmrFI	CCNGG	2 cut(s)	863, 1082
BmsI	GCATC	2 cut(s)	907, 1010
BpiI	GAAGAC	1 cut(s)	468
Bpu10I	CCTNAGC	1 cut(s)	743
Bpu1102I	GCTNAGC	1 cut(s)	609
BpuEI	CTTGAG	1 cut(s)	1249
BsaJI	CCNNGG	3 cut(s)	102, 832, 1058
BsaWI	WCCGGW	2 cut(s)	816, 1273
Bsc4I	CCNNNNNNNGG	3 cut(s)	629, 1230, 1404
Bse118I	RCCGGY	1 cut(s)	593
Bse1I	ACTGG	2 cut(s)	643, 1183
Bse3DI	GCAATG	2 cut(s)	502, 537
BseBI	CCWGG	2 cut(s)	863, 1082
BseDI	CCNNGG	3 cut(s)	102, 832, 1058
BseGI	GGATG	2 cut(s)	710, 858
BseLI	CCNNNNNNNGG	3 cut(s)	629, 1230, 1404
BseMI	GCAATG	2 cut(s)	502, 537
BseMII	CTCAG	4 cut(s)	708, 757, 1059, 1194
BseNI	ACTGG	2 cut(s)	643, 1183
BseRI	GAGGAG	6 cut(s)	132, 180, 243, 276, 309, 919
BseXI	GCAGC	2 cut(s)	141, 1206
BsgI	GTGCAG	1 cut(s)	1300
Bsh1236I	CGCG	3 cut(s)	373, 379, 385
Bsh1285I	CGRYCG	3 cut(s)	371, 377, 383
BshFI	GGCC	5 cut(s)	593, 1122, 1151, 1175, 1385
BshNI	GGYRCC	1 cut(s)	5
BsiEI	CGRYCG	3 cut(s)	371, 377, 383
BsiHKAI	GWGCWC	1 cut(s)	1106
BsiSI	CCGG	4 cut(s)	564, 594, 817, 1274
BslFI	GGGAC	2 cut(s)	683, 1380
BslI	CCNNNNNNNGG	3 cut(s)	629, 1230, 1404
BsmFI	GGGAC	2 cut(s)	683, 1380
BsmI	GAATGC	1 cut(s)	176
BsnI	GGCC	5 cut(s)	593, 1122, 1151, 1175, 1385
Bsp1286I	GDGCHC	1 cut(s)	1106
Bsp143I	GATC	5 cut(s)	499, 811, 1290, 1342, 1349
Bsp1720I	GCTNAGC	1 cut(s)	609
Bsp19I	CCATGG	2 cut(s)	832, 1058
BspANI	GGCC	5 cut(s)	593, 1122, 1151, 1175, 1385
BspCNI	CTCAG	4 cut(s)	707, 756, 1058, 1195
BspFNI	CGCG	3 cut(s)	373, 379, 385
BspLI	GGNNCC	3 cut(s)	7, 928, 975
BspPI	GGATC	1 cut(s)	494
BspT107I	GGYRCC	1 cut(s)	5
BsrBI	CCGCTC	1 cut(s)	1405
BsrDI	GCAATG	2 cut(s)	502, 537
BsrFI	RCCGGY	1 cut(s)	593
BsrI	ACTGG	2 cut(s)	643, 1183
BssAI	RCCGGY	1 cut(s)	593
BssECI	CCNNGG	3 cut(s)	102, 832, 1058
BssMI	GATC	5 cut(s)	499, 811, 1290, 1342, 1349
BssT1I	CCWWGG	3 cut(s)	102, 832, 1058
Bst2UI	CCWGG	2 cut(s)	863, 1082
Bst4CI	ACNGT	4 cut(s)	588, 632, 1198, 1394
Bst6I	CTCTTC	2 cut(s)	884, 1031
BstC8I	GCNNGC	3 cut(s)	200, 1144, 1217
BstDEI	CTNAG	8 cut(s)	609, 694, 743, 785, 808, 1045, 1203, 1380
BstDSI	CCRYGG	2 cut(s)	832, 1058
BstF5I	GGATG	2 cut(s)	710, 858
BstFNI	CGCG	3 cut(s)	373, 379, 385
BstKTI	GATC	5 cut(s)	502, 814, 1293, 1345, 1352
BstMBI	GATC	5 cut(s)	499, 811, 1290, 1342, 1349
BstMCI	CGRYCG	3 cut(s)	371, 377, 383
BstMWI	GCNNNNNNNGC	3 cut(s)	135, 1148, 1411
BstNI	CCWGG	2 cut(s)	863, 1082
BstSCI	CCNGG	2 cut(s)	861, 1080
BstSFI	CTRYAG	1 cut(s)	133
BstUI	CGCG	3 cut(s)	373, 379, 385
BstV1I	GCAGC	2 cut(s)	141, 1206
BstV2I	GAAGAC	1 cut(s)	468
BstX2I	RGATCY	1 cut(s)	811
BstXI	CCANNNNNNTGG	1 cut(s)	1190
BstYI	RGATCY	1 cut(s)	811
BsuRI	GGCC	5 cut(s)	593, 1122, 1151, 1175, 1385
BtgI	CCRYGG	2 cut(s)	832, 1058
BtgZI	GCGATG	1 cut(s)	315
BtsCI	GGATG	2 cut(s)	710, 858
BtsIMutI	CAGTG	2 cut(s)	1203, 1390
Cac8I	GCNNGC	3 cut(s)	200, 1144, 1217
CaiI	CAGNNNCTG	2 cut(s)	1202, 1392
Cfr10I	RCCGGY	1 cut(s)	593
Cfr13I	GGNCC	6 cut(s)	974, 1021, 1121, 1271, 1333, 1407
CpoI	CGGWCCG	1 cut(s)	1271
Csp6I	GTAC	3 cut(s)	6, 875, 1497
CspI	CGGWCCG	1 cut(s)	1271
CviAII	CATG	7 cut(s)	71, 486, 668, 776, 833, 1059, 1411
CviQI	GTAC	3 cut(s)	6, 875, 1497
DdeI	CTNAG	8 cut(s)	609, 694, 743, 785, 808, 1045, 1203, 1380
DpnI	GATC	5 cut(s)	501, 813, 1292, 1344, 1351
DpnII	GATC	5 cut(s)	499, 811, 1290, 1342, 1349
EaeI	YGGCCR	3 cut(s)	591, 1149, 1173
Eam1104I	CTCTTC	2 cut(s)	884, 1031
EarI	CTCTTC	2 cut(s)	884, 1031
EciI	GGCGGA	1 cut(s)	174
Eco130I	CCWWGG	3 cut(s)	102, 832, 1058
Eco47I	GGWCC	5 cut(s)	974, 1021, 1271, 1333, 1407
Eco57I	CTGAAG	1 cut(s)	12
EcoO109I	RGGNCCY	1 cut(s)	1021
EcoRII	CCWGG	2 cut(s)	861, 1080
EcoT14I	CCWWGG	3 cut(s)	102, 832, 1058
ErhI	CCWWGG	3 cut(s)	102, 832, 1058
FaeI	CATG	7 cut(s)	74, 489, 671, 779, 836, 1062, 1414
FaqI	GGGAC	2 cut(s)	683, 1380
FatI	CATG	7 cut(s)	70, 485, 667, 775, 832, 1058, 1410
FauI	CCCGC	2 cut(s)	207, 222
FbaI	TGATCA	1 cut(s)	1290
Fnu4HI	GCNGC	6 cut(s)	127, 130, 203, 242, 335, 1220
FokI	GGATG	2 cut(s)	697, 845
Fsp4HI	GCNGC	6 cut(s)	127, 130, 203, 242, 335, 1220
FspBI	CTAG	1 cut(s)	638
GluI	GCNGC	6 cut(s)	127, 130, 203, 242, 335, 1220
HaeIII	GGCC	5 cut(s)	593, 1122, 1151, 1175, 1385
HapII	CCGG	4 cut(s)	564, 594, 817, 1274
Hin1II	CATG	7 cut(s)	74, 489, 671, 779, 836, 1062, 1414
HincII	GTYRAC	1 cut(s)	571
HindII	GTYRAC	1 cut(s)	571
HindIII	AAGCTT	2 cut(s)	755, 1473
HinfI	GANTC	2 cut(s)	910, 1162
HpaII	CCGG	4 cut(s)	564, 594, 817, 1274
HphI	GGTGA	9 cut(s)	225, 240, 258, 273, 291, 306, 348, 514, 924
Hpy166II	GTNNAC	1 cut(s)	571
Hpy188I	TCNGA	3 cut(s)	402, 428, 1271
Hpy188III	TCNNGA	4 cut(s)	700, 914, 1228, 1315
Hpy8I	GTNNAC	1 cut(s)	571
Hpy99I	CGWCG	1 cut(s)	461
HpyAV	CCTTC	2 cut(s)	77, 629
HpyCH4III	ACNGT	4 cut(s)	588, 632, 1198, 1394
HpyCH4IV	ACGT	2 cut(s)	867, 1311
HpyCH4V	TGCA	6 cut(s)	67, 416, 507, 530, 1215, 1281
HpyF10VI	GCNNNNNNNGC	3 cut(s)	135, 1148, 1411
HpyF3I	CTNAG	8 cut(s)	609, 694, 743, 785, 808, 1045, 1203, 1380
HpySE526I	ACGT	2 cut(s)	867, 1311
Hsp92II	CATG	7 cut(s)	74, 489, 671, 779, 836, 1062, 1414
KpnI	GGTACC	1 cut(s)	9
Ksp22I	TGATCA	1 cut(s)	1290
Kzo9I	GATC	5 cut(s)	499, 811, 1290, 1342, 1349
LmnI	GCTCC	2 cut(s)	154, 932
Lsp1109I	GCAGC	2 cut(s)	141, 1206
LweI	GCATC	2 cut(s)	907, 1010
MaeI	CTAG	1 cut(s)	638
MaeII	ACGT	2 cut(s)	867, 1311
MaeIII	GTNAC	4 cut(s)	246, 279, 312, 645
MalI	GATC	5 cut(s)	501, 813, 1292, 1344, 1351
MbiI	CCGCTC	1 cut(s)	1405
MboI	GATC	5 cut(s)	499, 811, 1290, 1342, 1349
MboII	GAAGA	5 cut(s)	179, 473, 871, 1018, 1366
MfeI	CAATTG	1 cut(s)	1321
MflI	RGATCY	1 cut(s)	811
MhlI	GDGCHC	1 cut(s)	1106
MlsI	TGGCCA	2 cut(s)	1151, 1175
MluCI	AATT	8 cut(s)	13, 432, 509, 682, 717, 796, 826, 1321
MluNI	TGGCCA	2 cut(s)	1151, 1175
MmeI	TCCRAC	2 cut(s)	702, 1249
Mox20I	TGGCCA	2 cut(s)	1151, 1175
MscI	TGGCCA	2 cut(s)	1151, 1175
MseI	TTAA	6 cut(s)	720, 1077, 1115, 1338, 1487, 1503
Msp20I	TGGCCA	2 cut(s)	1151, 1175
MspI	CCGG	4 cut(s)	564, 594, 817, 1274
MspR9I	CCNGG	2 cut(s)	863, 1082
MunI	CAATTG	1 cut(s)	1321
Mva1269I	GAATGC	1 cut(s)	176
MvaI	CCWGG	2 cut(s)	863, 1082
MvnI	CGCG	3 cut(s)	373, 379, 385
MwoI	GCNNNNNNNGC	3 cut(s)	135, 1148, 1411
NcoI	CCATGG	2 cut(s)	832, 1058
NdeII	GATC	5 cut(s)	499, 811, 1290, 1342, 1349
NlaIII	CATG	7 cut(s)	74, 489, 671, 779, 836, 1062, 1414
NlaIV	GGNNCC	3 cut(s)	7, 928, 975
NmuCI	GTSAC	3 cut(s)	246, 279, 312
PctI	GAATGC	1 cut(s)	176
PfeI	GAWTC	2 cut(s)	910, 1162
PflFI	GACNNNGTC	1 cut(s)	1392
PfoI	TCCNGGA	1 cut(s)	861
PkrI	GCNGC	6 cut(s)	128, 131, 204, 243, 336, 1221
PpuMI	RGGWCCY	1 cut(s)	1021
Psp5II	RGGWCCY	1 cut(s)	1021
Psp6I	CCWGG	2 cut(s)	861, 1080
PspGI	CCWGG	2 cut(s)	861, 1080
PspN4I	GGNNCC	3 cut(s)	7, 928, 975
PspPI	GGNCC	6 cut(s)	974, 1021, 1121, 1271, 1333, 1407
PspPPI	RGGWCCY	1 cut(s)	1021
PstNI	CAGNNNCTG	2 cut(s)	1202, 1392
PsuI	RGATCY	1 cut(s)	811
PsyI	GACNNNGTC	1 cut(s)	1392
RsaI	GTAC	3 cut(s)	7, 876, 1498
RsaNI	GTAC	3 cut(s)	6, 875, 1497
Rsr2I	CGGWCCG	1 cut(s)	1271
RsrII	CGGWCCG	1 cut(s)	1271
SaqAI	TTAA	6 cut(s)	720, 1077, 1115, 1338, 1487, 1503
SatI	GCNGC	6 cut(s)	127, 130, 203, 242, 335, 1220
Sau3AI	GATC	5 cut(s)	499, 811, 1290, 1342, 1349
Sau96I	GGNCC	6 cut(s)	974, 1021, 1121, 1271, 1333, 1407
ScrFI	CCNGG	2 cut(s)	863, 1082
SduI	GDGCHC	1 cut(s)	1106
SfaNI	GCATC	2 cut(s)	907, 1010
SfcI	CTRYAG	1 cut(s)	133
SinI	GGWCC	5 cut(s)	974, 1021, 1271, 1333, 1407
SmlI	CTYRAG	1 cut(s)	1228
SmoI	CTYRAG	1 cut(s)	1228
Sse9I	AATT	8 cut(s)	13, 432, 509, 682, 717, 796, 826, 1321
SspMI	CTAG	1 cut(s)	638
StyD4I	CCNGG	2 cut(s)	861, 1080
StyI	CCWWGG	3 cut(s)	102, 832, 1058
TaaI	ACNGT	4 cut(s)	588, 632, 1198, 1394
TaiI	ACGT	2 cut(s)	870, 1314
TaqI	TCGA	2 cut(s)	459, 1095
TasI	AATT	8 cut(s)	13, 432, 509, 682, 717, 796, 826, 1321
TatI	WGTACW	1 cut(s)	874
TauI	GCSGC	4 cut(s)	129, 205, 244, 337
TfiI	GAWTC	2 cut(s)	910, 1162
Tru1I	TTAA	6 cut(s)	720, 1077, 1115, 1338, 1487, 1503
Tru9I	TTAA	6 cut(s)	720, 1077, 1115, 1338, 1487, 1503
TscAI	CASTG	2 cut(s)	1203, 1397
TseFI	GTSAC	3 cut(s)	246, 279, 312
TseI	GCWGC	2 cut(s)	129, 1219
Tsp45I	GTSAC	3 cut(s)	246, 279, 312
TspDTI	ATGAA	3 cut(s)	792, 847, 889
TspGWI	ACGGA	1 cut(s)	1506
TspRI	CASTG	2 cut(s)	1203, 1397
Tth111I	GACNNNGTC	1 cut(s)	1392
VpaK11BI	GGWCC	5 cut(s)	974, 1021, 1271, 1333, 1407
XapI	RAATTY	2 cut(s)	509, 796
XspI	CTAG	1 cut(s)	638

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.