FvH4_2g14550

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005259
Physical Location & Seq
Forward (+)
12769684 .. 12772158
2475 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g14550.t1

Sequence Viewer

Length: 705 bp
ATGGAGGAAGTAGAGGTAGTCATAGTAGGTGCAGGTCCTGCTGGTCTAGCAACCTCTGCATGTCTTAACCGCCTTAATATCTCAAATGTCATACTCGAAAGAGAAGATTGCTATGCTTCTCTTTGGAAGAAAAGGTCATATGATCGTTTGAAGCTTCACTTAGCAAAGCAATTTTGTGCACTACCCTACAAGCCCTTTCCTAAAGATGCACCCAGATATATGCCTAGGAGAGAGTTTATTCAATACCTAGATAATTATGTATCCACCTTCAACATAAACCCTCTTTACCACAGAGGTGTGGAGACCGCTTTCTTCAATGATTATGCAGGAAAATGGTATGTCGTTGTCAACAACACACGACAGGGTGTACAAGAAACCTACTGTGGGCAGTTTCTTGTGGTAGCAAGTGGTGAAAATGGTGAAGGCTATGTTCCTGAAATTAAAGGTTTGGATACCTTCAATGGTGAATTCATTCACTCGAGCAAGTATGACAATGGATTGAAGTACCGTGGGAAAGATGTGTTGGTTGTCGGATCAGGAAATTCCGGAATGGAAATTGCCTATGATTTATCTAATTACGGGGCAAATACTTCCATTGTTATTCGTAGCCTGACACATGTTCTTACCAAAGAAATTGTGTTCCTTGGAATGGTTCTGTCCAAATACCTTCCCGTTAAAGTGATCGATGGCATTGTTAAGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
FMO-like K_oxygenase Pyr_redox_3

Protein Analysis

235

Amino Acids

26.37

Weight (kDa)

8.22

Isoelectric Point (pI)

36.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 3 - 36 6.2e-06 FAD binding domain
FMO-like PF00743 5 - 210 9.3e-19 Flavin-binding monooxygenase-like
Pyr_redox_2 PF07992 5 - 202 1.1e-14 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_3 PF13738 7 - 203 2.9e-21 Pyridine nucleotide-disulphide oxidoreductase
Lys_Orn_oxgnase PF13434 71 - 200 1.8e-12 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 23
AccIII TCCGGA 1 cut(s) 545
AciI CCGC 2 cut(s) 70, 306
AclWI GGATC 1 cut(s) 541
AcsI RAATTY 2 cut(s) 467, 541
AfaI GTAC 2 cut(s) 369, 506
AfiI CCNNNNNNNGG 2 cut(s) 384, 649
AflIII ACRYGT 1 cut(s) 616
AgsI TTSAA 6 cut(s) 151, 242, 271, 316, 460, 502
AjuI GAANNNNNNNTTGG 2 cut(s) 506, 538
AleI CACNNNNGTG 1 cut(s) 294
AluBI AGCT 1 cut(s) 154
AluI AGCT 1 cut(s) 154
Alw21I GWGCWC 1 cut(s) 181
Alw26I GTCTC 1 cut(s) 296
Alw44I GTGCAC 1 cut(s) 177
AlwI GGATC 1 cut(s) 541
AlwNI CAGNNNCTG 1 cut(s) 38
Ama87I CYCGRG 1 cut(s) 478
Aor13HI TCCGGA 1 cut(s) 545
ApaLI GTGCAC 1 cut(s) 177
ApoI RAATTY 2 cut(s) 467, 541
Asp700I GAANNNNTTC 1 cut(s) 471
AspA2I CCTAGG 1 cut(s) 224
AspS9I GGNCC 1 cut(s) 35
AsuHPI GGTGA 3 cut(s) 422, 431, 476
AvaI CYCGRG 1 cut(s) 478
AvaII GGWCC 1 cut(s) 35
AvrII CCTAGG 1 cut(s) 224
BaeGI GKGCMC 1 cut(s) 181
Bbv12I GWGCWC 1 cut(s) 181
BccI CCATC 1 cut(s) 680
BciVI GTATCC 2 cut(s) 271, 445
BcoDI GTCTC 1 cut(s) 296
BfaI CTAG 3 cut(s) 47, 225, 248
BfuAI ACCTGC 1 cut(s) 23
BfuI GTATCC 2 cut(s) 271, 445
BlnI CCTAGG 1 cut(s) 224
Bme18I GGWCC 1 cut(s) 35
BmeT110I CYCGRG 1 cut(s) 478
BmgT120I GGNCC 1 cut(s) 35
BmsI GCATC 1 cut(s) 196
Bsa29I ATCGAT 1 cut(s) 684
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 3 cut(s) 224, 508, 643
BsaWI WCCGGW 1 cut(s) 545
Bsc4I CCNNNNNNNGG 2 cut(s) 384, 649
BseAI TCCGGA 1 cut(s) 545
BseCI ATCGAT 1 cut(s) 684
BseDI CCNNGG 3 cut(s) 224, 508, 643
BseLI CCNNNNNNNGG 2 cut(s) 384, 649
BseSI GKGCMC 1 cut(s) 181
BsgI GTGCAG 1 cut(s) 51
BshVI ATCGAT 1 cut(s) 684
BsiHKAI GWGCWC 1 cut(s) 181
BsiHKCI CYCGRG 1 cut(s) 478
BsiSI CCGG 1 cut(s) 546
BslI CCNNNNNNNGG 2 cut(s) 384, 649
BsmAI GTCTC 1 cut(s) 296
Bso31I GGTCTC 1 cut(s) 296
BsoBI CYCGRG 1 cut(s) 478
Bsp1286I GDGCHC 1 cut(s) 181
Bsp13I TCCGGA 1 cut(s) 545
Bsp1407I TGTACA 1 cut(s) 367
Bsp143I GATC 3 cut(s) 142, 533, 681
BspACI CCGC 2 cut(s) 70, 306
BspDI ATCGAT 1 cut(s) 684
BspEI TCCGGA 1 cut(s) 545
BspMI ACCTGC 1 cut(s) 23
BspPI GGATC 1 cut(s) 541
BspTNI GGTCTC 1 cut(s) 296
BsrGI TGTACA 1 cut(s) 367
BssECI CCNNGG 3 cut(s) 224, 508, 643
BssMI GATC 3 cut(s) 142, 533, 681
BssT1I CCWWGG 2 cut(s) 224, 643
Bst4CI ACNGT 2 cut(s) 383, 509
BstAPI GCANNNNNTGC 2 cut(s) 38, 56
BstAUI TGTACA 1 cut(s) 367
BstDEI CTNAG 1 cut(s) 160
BstDSI CCRYGG 1 cut(s) 508
BstKTI GATC 3 cut(s) 145, 536, 684
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 3 cut(s) 142, 533, 681
BstMWI GCNNNNNNNGC 3 cut(s) 38, 47, 56
BstNSI RCATGY 2 cut(s) 63, 620
BstSLI GKGCMC 1 cut(s) 181
Bsu15I ATCGAT 1 cut(s) 684
BsuI GTATCC 2 cut(s) 271, 445
BsuTUI ATCGAT 1 cut(s) 684
BtgI CCRYGG 1 cut(s) 508
BveI ACCTGC 1 cut(s) 23
CaiI CAGNNNCTG 1 cut(s) 38
Cfr13I GGNCC 1 cut(s) 35
ClaI ATCGAT 1 cut(s) 684
Csp6I GTAC 2 cut(s) 368, 505
CviAII CATG 2 cut(s) 60, 617
CviJI RGCY 4 cut(s) 154, 193, 426, 609
CviKI_1 RGCY 4 cut(s) 154, 193, 426, 609
CviQI GTAC 2 cut(s) 368, 505
DdeI CTNAG 1 cut(s) 160
DpnI GATC 3 cut(s) 144, 535, 683
DpnII GATC 3 cut(s) 142, 533, 681
Eco130I CCWWGG 2 cut(s) 224, 643
Eco31I GGTCTC 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 35
Eco88I CYCGRG 1 cut(s) 478
EcoO109I RGGNCCY 1 cut(s) 35
EcoRI GAATTC 1 cut(s) 467
EcoT14I CCWWGG 2 cut(s) 224, 643
ErhI CCWWGG 2 cut(s) 224, 643
FaeI CATG 2 cut(s) 63, 620
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FatI CATG 2 cut(s) 59, 616
FauNDI CATATG 1 cut(s) 139
FspBI CTAG 3 cut(s) 47, 225, 248
HapII CCGG 1 cut(s) 546
Hin1II CATG 2 cut(s) 63, 620
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 152
HpaII CCGG 1 cut(s) 546
HphI GGTGA 3 cut(s) 422, 431, 476
Hpy166II GTNNAC 3 cut(s) 179, 349, 368
Hpy188I TCNGA 1 cut(s) 533
Hpy188III TCNNGA 3 cut(s) 434, 537, 546
Hpy8I GTNNAC 3 cut(s) 179, 349, 368
HpyAV CCTTC 4 cut(s) 277, 416, 466, 677
HpyCH4III ACNGT 2 cut(s) 383, 509
HpyCH4V TGCA 5 cut(s) 32, 59, 179, 209, 326
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 47, 56
HpyF3I CTNAG 1 cut(s) 160
Hsp92II CATG 2 cut(s) 63, 620
Kpn2I TCCGGA 1 cut(s) 545
Kzo9I GATC 3 cut(s) 142, 533, 681
LweI GCATC 1 cut(s) 196
MaeI CTAG 3 cut(s) 47, 225, 248
MalI GATC 3 cut(s) 144, 535, 683
MboI GATC 3 cut(s) 142, 533, 681
MboII GAAGA 3 cut(s) 116, 139, 304
MhlI GDGCHC 1 cut(s) 181
MluCI AATT 8 cut(s) 170, 253, 438, 467, 541, 555, 574, 633
MmeI TCCRAC 1 cut(s) 511
MnlI CCTC 4 cut(s) 7, 64, 287, 291
MroI TCCGGA 1 cut(s) 545
MroXI GAANNNNTTC 1 cut(s) 471
MseI TTAA 6 cut(s) 66, 75, 441, 675, 696, 703
MslI CAYNNNNRTG 1 cut(s) 294
MspI CCGG 1 cut(s) 546
MwoI GCNNNNNNNGC 3 cut(s) 38, 47, 56
NdeI CATATG 1 cut(s) 139
NdeII GATC 3 cut(s) 142, 533, 681
NlaIII CATG 2 cut(s) 63, 620
NspI RCATGY 2 cut(s) 63, 620
OliI CACNNNNGTG 1 cut(s) 294
PaeR7I CTCGAG 1 cut(s) 478
PciI ACATGT 1 cut(s) 616
PdmI GAANNNNTTC 1 cut(s) 471
PpuMI RGGWCCY 1 cut(s) 35
PscI ACATGT 1 cut(s) 616
Psp5II RGGWCCY 1 cut(s) 35
PspPI GGNCC 1 cut(s) 35
PspPPI RGGWCCY 1 cut(s) 35
PspXI VCTCGAGB 1 cut(s) 478
PstNI CAGNNNCTG 1 cut(s) 38
RsaI GTAC 2 cut(s) 369, 506
RsaNI GTAC 2 cut(s) 368, 505
RseI CAYNNNNRTG 1 cut(s) 294
SaqAI TTAA 6 cut(s) 66, 75, 441, 675, 696, 703
Sau3AI GATC 3 cut(s) 142, 533, 681
Sau96I GGNCC 1 cut(s) 35
SduI GDGCHC 1 cut(s) 181
SfaNI GCATC 1 cut(s) 196
Sfr274I CTCGAG 1 cut(s) 478
SinI GGWCC 1 cut(s) 35
SlaI CTCGAG 1 cut(s) 478
SmiMI CAYNNNNRTG 1 cut(s) 294
SmlI CTYRAG 1 cut(s) 478
SmoI CTYRAG 1 cut(s) 478
Sse9I AATT 8 cut(s) 170, 253, 438, 467, 541, 555, 574, 633
SsiI CCGC 2 cut(s) 70, 306
SspMI CTAG 3 cut(s) 47, 225, 248
StyI CCWWGG 2 cut(s) 224, 643
TaaI ACNGT 2 cut(s) 383, 509
TaqI TCGA 3 cut(s) 96, 479, 684
TasI AATT 8 cut(s) 170, 253, 438, 467, 541, 555, 574, 633
TatI WGTACW 1 cut(s) 367
Tru1I TTAA 6 cut(s) 66, 75, 441, 675, 696, 703
Tru9I TTAA 6 cut(s) 66, 75, 441, 675, 696, 703
TspDTI ATGAA 1 cut(s) 460
VneI GTGCAC 1 cut(s) 177
VpaK11BI GGWCC 1 cut(s) 35
XapI RAATTY 2 cut(s) 467, 541
XceI RCATGY 2 cut(s) 63, 620
XhoI CTCGAG 1 cut(s) 478
XmaJI CCTAGG 1 cut(s) 224
XmnI GAANNNNTTC 1 cut(s) 471
XspI CTAG 3 cut(s) 47, 225, 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.