FvH4_2g15120

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005259
Physical Location & Seq
Forward (+)
13248886 .. 13251730
2845 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g15120.t1

Sequence Viewer

Length: 1113 bp
ATGGAGGAAGTAGATGTAGTCATAATAGGTGCAGGTCCTGCTGGTTTAGCAACCTCTGCATGTCTGAACCGCCTCAAAATCTCCAATGTCGTGCTCGAAAGAGAAGATTGCTGTGCTTCTCTTTGGAAGAACAGGTCATATGATCGTTTGAAGCTTCACTTAGCAAAGCAATTCTGTGAACTACCCTACATGCCTTTCCCTGAAAACACACCCACATATGTTCCTAGGAAAGAGTTTATTCAATACCTAGATACTTATGTGTCCACTTTCAAGATAAACCCTCTGTACCACAGGAGTGTGGAGACTGCCTCGTTTGATGAGGATGTGGGTAAATGGTGTGTCCTTGTCAACAACACAGAACTGGGTGTACAAGAAAGTTACTACGCAAAGTTTCTCGTGGCAGCGACCGGTGAAAATGGTGAAGGCTATCTTCCTGAAACAAACGGTTTGGGTAGCTTTAAAGGCGAAATCATTCATTCGAGTGAGTATGGAAATGGCAACAAGTACCGTGGGAAAAATGTTTTAGTTGTCGGATCTGGGAACTCCGGAATGGAAATTGCCTACGACTTATCAAATTCCGGCGCTAATACTTCTATTGTACATGTTCTTACCAAGGAGATCGTGTTCATTGGAATGGTTCTGTCAAAGTACCTTCCCATTATGGTTGTTGATGGCATTGTCATGTTCTTAACCAAGCTGAAATTTGGAAATTTATCCAAGTATGGGATTCAAAATCCAAAAATGGGGCCATTTCATATCAAGCAAAACGAGGGTCACTCCCCCATCATTGATGTTGGAACTATCAAAAAGATCAAGAGTGGAGATATACAGGTTTTGCCATCCATAACAAACATAGAAGGAAATGAAATCCGGTTCGAGAATGGATACCTTAAATGGTATGATGCTATAGTATTTGCTACAGGCTATAAGAGCACAGTGCTAAAGTGGCTCAAGGATGAAAACAACCTCTTTGGTGACAATGGAATGCCCAAGAAAAGTTTTCCCAACCACTGGAAGTCACAAAATGGCCTTTACTGTGCTGGATTCTCCAAGCGTGGATTGTTTGGCATATCATACGATGCACAACATATATCAAATGATATAAATAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
FMO-like K_oxygenase Pyr_redox_3

Protein Analysis

371

Amino Acids

41.28

Weight (kDa)

7.59

Isoelectric Point (pI)

28.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_2 PF07992 5 - 205 3.9e-14 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 6 - 311 5.5e-22 Flavin-binding monooxygenase-like
Pyr_redox_3 PF13738 7 - 201 3.6e-21 Pyridine nucleotide-disulphide oxidoreductase
Lys_Orn_oxgnase PF13434 68 - 204 3.6e-14 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 23
AccB7I CCANNNNNTGG 1 cut(s) 1011
AccIII TCCGGA 1 cut(s) 545
AciI CCGC 1 cut(s) 70
AclWI GGATC 1 cut(s) 541
AcsI RAATTY 3 cut(s) 574, 701, 709
AfaI GTAC 5 cut(s) 287, 369, 506, 600, 650
AfiI CCNNNNNNNGG 3 cut(s) 723, 743, 1011
AflIII ACRYGT 1 cut(s) 601
AgeI ACCGGT 1 cut(s) 407
AgsI TTSAA 4 cut(s) 151, 242, 271, 731
AleI CACNNNNGTG 1 cut(s) 294
AloI GAACNNNNNNTCC 2 cut(s) 608, 640
AluBI AGCT 3 cut(s) 154, 456, 697
AluI AGCT 3 cut(s) 154, 456, 697
Alw21I GWGCWC 2 cut(s) 96, 935
Alw26I GTCTC 1 cut(s) 296
AlwI GGATC 1 cut(s) 541
AlwNI CAGNNNCTG 1 cut(s) 38
Aor13HI TCCGGA 1 cut(s) 545
AoxI GGCC 2 cut(s) 746, 1027
ApeKI GCWGC 1 cut(s) 401
ApoI RAATTY 3 cut(s) 574, 701, 709
ArsI GACNNNNNNTTYG 2 cut(s) 757, 789
AsiGI ACCGGT 1 cut(s) 407
Asp700I GAANNNNTTC 1 cut(s) 471
AspA2I CCTAGG 1 cut(s) 224
AspLEI GCGC 1 cut(s) 584
AspS9I GGNCC 2 cut(s) 35, 746
AsuHPI GGTGA 3 cut(s) 422, 431, 986
AvaII GGWCC 1 cut(s) 35
AvrII CCTAGG 1 cut(s) 224
BaeI ACNNNNGTAYC 2 cut(s) 243, 276
BauI CACGAG 1 cut(s) 395
Bbv12I GWGCWC 2 cut(s) 96, 935
BbvI GCAGC 1 cut(s) 413
BccI CCATC 3 cut(s) 665, 791, 847
BciVI GTATCC 1 cut(s) 878
BcoDI GTCTC 1 cut(s) 296
BfaI CTAG 2 cut(s) 225, 248
BfmI CTRYAG 2 cut(s) 906, 918
BfoI RGCGCY 1 cut(s) 585
BfuAI ACCTGC 1 cut(s) 23
BfuI GTATCC 1 cut(s) 878
BisI GCNGC 1 cut(s) 402
BlnI CCTAGG 1 cut(s) 224
BlsI GCNGC 1 cut(s) 403
Bme18I GGWCC 1 cut(s) 35
BmgT120I GGNCC 2 cut(s) 35, 746
BmiI GGNNCC 1 cut(s) 747
BmrI ACTGGG 1 cut(s) 371
BmsI GCATC 2 cut(s) 892, 1069
BmuI ACTGGG 1 cut(s) 371
BplI GAGNNNNNCTC 4 cut(s) 293, 325, 761, 793
BpuEI CTTGAG 1 cut(s) 935
BsaJI CCNNGG 3 cut(s) 224, 508, 612
BsaWI WCCGGW 3 cut(s) 407, 545, 870
BsaXI ACNNNNNCTCC 2 cut(s) 608, 638
Bsc4I CCNNNNNNNGG 3 cut(s) 723, 743, 1011
Bse118I RCCGGY 1 cut(s) 407
Bse1I ACTGG 2 cut(s) 366, 1016
BseAI TCCGGA 1 cut(s) 545
BseDI CCNNGG 3 cut(s) 224, 508, 612
BseGI GGATG 3 cut(s) 328, 839, 961
BseLI CCNNNNNNNGG 3 cut(s) 723, 743, 1011
BseNI ACTGG 2 cut(s) 366, 1016
BseXI GCAGC 1 cut(s) 413
BsgI GTGCAG 1 cut(s) 51
Bsh1285I CGRYCG 1 cut(s) 408
BshFI GGCC 2 cut(s) 748, 1029
BshTI ACCGGT 1 cut(s) 407
BsiEI CGRYCG 1 cut(s) 408
BsiHKAI GWGCWC 2 cut(s) 96, 935
BsiSI CCGG 4 cut(s) 408, 546, 579, 871
BslI CCNNNNNNNGG 3 cut(s) 723, 743, 1011
BsmAI GTCTC 1 cut(s) 296
BsmI GAATGC 1 cut(s) 990
BsnI GGCC 2 cut(s) 748, 1029
Bsp1286I GDGCHC 2 cut(s) 96, 935
Bsp13I TCCGGA 1 cut(s) 545
Bsp1407I TGTACA 2 cut(s) 367, 598
Bsp143I GATC 4 cut(s) 142, 533, 618, 810
BspACI CCGC 1 cut(s) 70
BspANI GGCC 2 cut(s) 748, 1029
BspEI TCCGGA 1 cut(s) 545
BspLI GGNNCC 1 cut(s) 747
BspMI ACCTGC 1 cut(s) 23
BspPI GGATC 1 cut(s) 541
BsrFI RCCGGY 1 cut(s) 407
BsrGI TGTACA 2 cut(s) 367, 598
BsrI ACTGG 2 cut(s) 366, 1016
BssAI RCCGGY 1 cut(s) 407
BssECI CCNNGG 3 cut(s) 224, 508, 612
BssMI GATC 4 cut(s) 142, 533, 618, 810
BssSI CACGAG 1 cut(s) 395
BssT1I CCWWGG 2 cut(s) 224, 612
Bst2BI CACGAG 1 cut(s) 395
Bst4CI ACNGT 4 cut(s) 446, 509, 937, 1037
BstAPI GCANNNNNTGC 2 cut(s) 38, 56
BstAUI TGTACA 2 cut(s) 367, 598
BstDEI CTNAG 1 cut(s) 160
BstDSI CCRYGG 1 cut(s) 508
BstF5I GGATG 3 cut(s) 328, 839, 961
BstH2I RGCGCY 1 cut(s) 585
BstHHI GCGC 1 cut(s) 584
BstKTI GATC 4 cut(s) 145, 536, 621, 813
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 4 cut(s) 142, 533, 618, 810
BstMCI CGRYCG 1 cut(s) 408
BstMWI GCNNNNNNNGC 6 cut(s) 38, 47, 56, 462, 930, 946
BstNSI RCATGY 3 cut(s) 63, 193, 605
BstSFI CTRYAG 2 cut(s) 906, 918
BstV1I GCAGC 1 cut(s) 413
BstX2I RGATCY 1 cut(s) 533
BstYI RGATCY 1 cut(s) 533
BsuI GTATCC 1 cut(s) 878
BsuRI GGCC 2 cut(s) 748, 1029
BtgI CCRYGG 1 cut(s) 508
BtsCI GGATG 3 cut(s) 328, 839, 961
BtsIMutI CAGTG 2 cut(s) 942, 1009
BveI ACCTGC 1 cut(s) 23
CaiI CAGNNNCTG 1 cut(s) 38
CfoI GCGC 1 cut(s) 584
Cfr10I RCCGGY 1 cut(s) 407
Cfr13I GGNCC 2 cut(s) 35, 746
Csp6I GTAC 5 cut(s) 286, 368, 505, 599, 649
CspAI ACCGGT 1 cut(s) 407
CspCI CAANNNNNGTGG 2 cut(s) 490, 525
CviAII CATG 4 cut(s) 60, 190, 602, 682
CviJI RGCY 8 cut(s) 154, 426, 456, 697, 748, 924, 949, 1029
CviKI_1 RGCY 8 cut(s) 154, 426, 456, 697, 748, 924, 949, 1029
CviQI GTAC 5 cut(s) 286, 368, 505, 599, 649
DdeI CTNAG 1 cut(s) 160
DpnI GATC 4 cut(s) 144, 535, 620, 812
DpnII GATC 4 cut(s) 142, 533, 618, 810
DraI TTTAAA 1 cut(s) 460
Eco130I CCWWGG 2 cut(s) 224, 612
Eco47I GGWCC 1 cut(s) 35
EcoO109I RGGNCCY 1 cut(s) 35
EcoT14I CCWWGG 2 cut(s) 224, 612
ErhI CCWWGG 2 cut(s) 224, 612
FaeI CATG 4 cut(s) 63, 193, 605, 685
FalI AAGNNNNNCTT 4 cut(s) 143, 175, 414, 446
FatI CATG 4 cut(s) 59, 189, 601, 681
FauNDI CATATG 2 cut(s) 139, 217
Fnu4HI GCNGC 1 cut(s) 402
FokI GGATG 3 cut(s) 335, 826, 968
Fsp4HI GCNGC 1 cut(s) 402
FspBI CTAG 2 cut(s) 225, 248
GlaI GCGC 1 cut(s) 583
GluI GCNGC 1 cut(s) 402
HaeII RGCGCY 1 cut(s) 585
HaeIII GGCC 2 cut(s) 748, 1029
HapII CCGG 4 cut(s) 408, 546, 579, 871
HhaI GCGC 1 cut(s) 584
Hin1II CATG 4 cut(s) 63, 193, 605, 685
Hin6I GCGC 1 cut(s) 582
HinP1I GCGC 1 cut(s) 582
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 152
HinfI GANTC 2 cut(s) 727, 1044
HpaII CCGG 4 cut(s) 408, 546, 579, 871
HphI GGTGA 3 cut(s) 422, 431, 986
Hpy166II GTNNAC 4 cut(s) 179, 264, 349, 368
Hpy188I TCNGA 2 cut(s) 66, 533
Hpy188III TCNNGA 5 cut(s) 271, 434, 546, 814, 877
Hpy8I GTNNAC 4 cut(s) 179, 264, 349, 368
HpyAV CCTTC 3 cut(s) 416, 662, 851
HpyCH4III ACNGT 4 cut(s) 446, 509, 937, 1037
HpyCH4V TGCA 3 cut(s) 32, 59, 1082
HpyF10VI GCNNNNNNNGC 6 cut(s) 38, 47, 56, 462, 930, 946
HpyF3I CTNAG 1 cut(s) 160
Hsp92II CATG 4 cut(s) 63, 193, 605, 685
HspAI GCGC 1 cut(s) 582
Kpn2I TCCGGA 1 cut(s) 545
Kzo9I GATC 4 cut(s) 142, 533, 618, 810
Lsp1109I GCAGC 1 cut(s) 413
LweI GCATC 2 cut(s) 892, 1069
MaeI CTAG 2 cut(s) 225, 248
MaeIII GTNAC 4 cut(s) 377, 773, 974, 1017
MalI GATC 4 cut(s) 144, 535, 620, 812
MboI GATC 4 cut(s) 142, 533, 618, 810
MboII GAAGA 3 cut(s) 116, 139, 422
MflI RGATCY 1 cut(s) 533
MhlI GDGCHC 2 cut(s) 96, 935
MluCI AATT 5 cut(s) 170, 555, 574, 701, 709
MmeI TCCRAC 2 cut(s) 511, 775
MnlI CCTC 7 cut(s) 64, 83, 291, 313, 319, 763, 977
MroI TCCGGA 1 cut(s) 545
MroXI GAANNNNTTC 1 cut(s) 471
MseI TTAA 3 cut(s) 459, 689, 891
MslI CAYNNNNRTG 4 cut(s) 294, 480, 632, 680
MspI CCGG 4 cut(s) 408, 546, 579, 871
Mva1269I GAATGC 1 cut(s) 990
MwoI GCNNNNNNNGC 6 cut(s) 38, 47, 56, 462, 930, 946
NdeI CATATG 2 cut(s) 139, 217
NdeII GATC 4 cut(s) 142, 533, 618, 810
NlaIII CATG 4 cut(s) 63, 193, 605, 685
NlaIV GGNNCC 1 cut(s) 747
NmuCI GTSAC 3 cut(s) 773, 974, 1017
NspI RCATGY 3 cut(s) 63, 193, 605
OliI CACNNNNGTG 1 cut(s) 294
PciI ACATGT 1 cut(s) 601
PctI GAATGC 1 cut(s) 990
PdmI GAANNNNTTC 1 cut(s) 471
PfeI GAWTC 2 cut(s) 727, 1044
PflMI CCANNNNNTGG 1 cut(s) 1011
PinAI ACCGGT 1 cut(s) 407
PkrI GCNGC 1 cut(s) 403
PpuMI RGGWCCY 1 cut(s) 35
PscI ACATGT 1 cut(s) 601
Psp5II RGGWCCY 1 cut(s) 35
PspN4I GGNNCC 1 cut(s) 747
PspPI GGNCC 2 cut(s) 35, 746
PspPPI RGGWCCY 1 cut(s) 35
PsrI GAACNNNNNNTAC 2 cut(s) 351, 383
PstNI CAGNNNCTG 1 cut(s) 38
PsuI RGATCY 1 cut(s) 533
RsaI GTAC 5 cut(s) 287, 369, 506, 600, 650
RsaNI GTAC 5 cut(s) 286, 368, 505, 599, 649
RseI CAYNNNNRTG 4 cut(s) 294, 480, 632, 680
SaqAI TTAA 3 cut(s) 459, 689, 891
SatI GCNGC 1 cut(s) 402
Sau3AI GATC 4 cut(s) 142, 533, 618, 810
Sau96I GGNCC 2 cut(s) 35, 746
SduI GDGCHC 2 cut(s) 96, 935
SfaNI GCATC 2 cut(s) 892, 1069
SfcI CTRYAG 2 cut(s) 906, 918
SinI GGWCC 1 cut(s) 35
SmiMI CAYNNNNRTG 4 cut(s) 294, 480, 632, 680
SmlI CTYRAG 1 cut(s) 950
SmoI CTYRAG 1 cut(s) 950
Sse9I AATT 5 cut(s) 170, 555, 574, 701, 709
SsiI CCGC 1 cut(s) 70
SspMI CTAG 2 cut(s) 225, 248
StyI CCWWGG 2 cut(s) 224, 612
TaaI ACNGT 4 cut(s) 446, 509, 937, 1037
TaqI TCGA 3 cut(s) 96, 479, 876
TasI AATT 5 cut(s) 170, 555, 574, 701, 709
TatI WGTACW 2 cut(s) 367, 598
TfiI GAWTC 2 cut(s) 727, 1044
Tru1I TTAA 3 cut(s) 459, 689, 891
Tru9I TTAA 3 cut(s) 459, 689, 891
TscAI CASTG 2 cut(s) 942, 1016
TseFI GTSAC 3 cut(s) 773, 974, 1017
TseI GCWGC 1 cut(s) 401
Tsp45I GTSAC 3 cut(s) 773, 974, 1017
TspDTI ATGAA 5 cut(s) 464, 616, 743, 879, 972
TspRI CASTG 2 cut(s) 942, 1016
Van91I CCANNNNNTGG 1 cut(s) 1011
VpaK11BI GGWCC 1 cut(s) 35
XapI RAATTY 3 cut(s) 574, 701, 709
XceI RCATGY 3 cut(s) 63, 193, 605
XmaJI CCTAGG 1 cut(s) 224
XmnI GAANNNNTTC 1 cut(s) 471
XspI CTAG 2 cut(s) 225, 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.