Rh3DG294100

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0005259
Physical Location & Seq
Reverse (-)
30533313 .. 30539649
6337 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG294100.1

Sequence Viewer

Length: 1200 bp
ATGAAGTTCCTCACGAACGTCGCCAGAATGGAGGAAGTAGAGGTAGTCATAGTAGGTGCAGGTCCTGCTGGTCTAGCAACCTCTGCATGTCTTAACCGCCTCAATATCTCAAATGTCATACTCGAAAGAGAAGATTGCCATGCTTCTCTTTGGAAGAGTAGGACATATGATCGTTTGAAGCTTCACTTAGCAAAGCAATTTTGTGAACTACCCTATATGCCCTTTCCTAAAAATGCACCCACATATATTCCTAGGAGAGAGTTTGTTCAATACCTGGATACTTACGTATCCACCTTCAACATAAACCCTATGTACCACAGAAGTGTGGAGACTGCTTTTTACAATGAGAATGTAGAAAAATGGTATGTCGTAGTCAACAACACACGATTAGGTGTAAAAGAAACATATTATGGGAAGTTTCTTGTGGTAGCAAGTGGTGAAAATGGTGAAGGCTATGTTCCTGAAACAGAAGGTTTGGATACCTTTAACGGCGAATACATTCATTCGAGCAAGTATGAAAATGGCATGAAGTACCGTGGAAAAGATGTTTTGGTTGTCGGATCAGGAAATTCGGGAATGGAAATTGCCTACGACTTATCTAATTATGGCGCAAATACTTTCATTGTTGTTCGTAGCCCGGTACATGTTCTTACCAAAGAAATTGTGTTCTTTGGAATGGTTTTATCCAAATATCTTCCGGTTAAAGTTATCGATGGCATTGTCCTGATCTTAGGGAAATTGAAATTTGGAAATTTATCGAAGTATGGAATTCATAAACCAAAAATAGGGCCATTTTATCTCAAGGAACACGAGGGTCAAGCCCCCATCATCGATGTTGGGACCATTGAAAAGATCAAGAGTGACGAAATCAAGGTTTTGCCTTCCATAACAAGCATAGAAGGAAATGAAATCCGATTTGAGAATGGCTACCTTAAAAGTTATGATGCTATAATATTTGCTACGGGCTATAAAAGCACTGTGCTAAAGTGGCTCAAGGATGACAATCATCTCTTTAATGACAATGGAATGCCGAATAAAAGTTTTCCGAACCACTGGAAGTCAGAAAATGGTCTTTACTGTGTTGGTTTCTCAAGGCGTGGACTATTTGGAATTTCGCATGATGCACTAAAAATAACAAATGATATCAATCATTCTCTCTTGGTCGGAACAAAAAGGGGATTTGATCATTCAGAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
FMO-like K_oxygenase Pyr_redox_3

Protein Analysis

399

Amino Acids

45.12

Weight (kDa)

7.67

Isoelectric Point (pI)

33.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 12 - 56 2.7e-06 FAD binding domain
FMO-like PF00743 14 - 325 3.6e-21 Flavin-binding monooxygenase-like
Pyr_redox_2 PF07992 14 - 213 1.8e-12 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_3 PF13738 16 - 213 9.2e-23 Pyridine nucleotide-disulphide oxidoreductase
Lys_Orn_oxgnase PF13434 77 - 205 6.4e-13 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 50
AciI CCGC 1 cut(s) 97
AclWI GGATC 1 cut(s) 568
AcsI RAATTY 5 cut(s) 568, 743, 751, 768, 1110
AfaI GTAC 3 cut(s) 314, 533, 642
AfiI CCNNNNNNNGG 1 cut(s) 785
AflIII ACRYGT 1 cut(s) 643
AgsI TTSAA 5 cut(s) 178, 269, 298, 742, 848
AjnI CCWGG 1 cut(s) 273
AleI CACNNNNGTG 1 cut(s) 321
AluBI AGCT 1 cut(s) 181
AluI AGCT 1 cut(s) 181
Alw26I GTCTC 1 cut(s) 323
AlwI GGATC 1 cut(s) 568
AlwNI CAGNNNCTG 1 cut(s) 65
AoxI GGCC 1 cut(s) 788
ApoI RAATTY 5 cut(s) 568, 743, 751, 768, 1110
Asp700I GAANNNNTTC 1 cut(s) 498
AspA2I CCTAGG 1 cut(s) 251
AspLEI GCGC 1 cut(s) 611
AspS9I GGNCC 3 cut(s) 62, 788, 840
AsuC2I CCSGG 1 cut(s) 638
AsuHPI GGTGA 2 cut(s) 449, 458
AvaII GGWCC 2 cut(s) 62, 840
AvrII CCTAGG 1 cut(s) 251
BaeI ACNNNNGTAYC 4 cut(s) 270, 270, 303, 303
BauI CACGAG 1 cut(s) 809
BccI CCATC 2 cut(s) 707, 833
BceAI ACGGC 1 cut(s) 505
BciT130I CCWGG 1 cut(s) 275
BciVI GTATCC 3 cut(s) 271, 298, 472
BclI TGATCA 1 cut(s) 1183
BcnI CCSGG 1 cut(s) 638
BcoDI GTCTC 1 cut(s) 323
BfaI CTAG 2 cut(s) 74, 252
BfuAI ACCTGC 1 cut(s) 50
BfuI GTATCC 3 cut(s) 271, 298, 472
BlnI CCTAGG 1 cut(s) 251
Bme1390I CCNGG 2 cut(s) 275, 638
Bme18I GGWCC 2 cut(s) 62, 840
BmgT120I GGNCC 3 cut(s) 62, 788, 840
BmiI GGNNCC 1 cut(s) 841
BmrFI CCNGG 2 cut(s) 275, 638
BmsI GCATC 2 cut(s) 934, 1111
BpuEI CTTGAG 3 cut(s) 785, 977, 1075
BpuMI CCSGG 1 cut(s) 638
Bsa29I ATCGAT 2 cut(s) 711, 831
BsaAI YACGTR 1 cut(s) 286
BsaBI GATNNNNATC 2 cut(s) 1002, 1146
BsaJI CCNNGG 2 cut(s) 251, 535
BsaWI WCCGGW 1 cut(s) 697
Bsc4I CCNNNNNNNGG 1 cut(s) 785
Bse1I ACTGG 1 cut(s) 1058
Bse8I GATNNNNATC 2 cut(s) 1002, 1146
BseBI CCWGG 1 cut(s) 275
BseCI ATCGAT 2 cut(s) 711, 831
BseDI CCNNGG 2 cut(s) 251, 535
BseGI GGATG 1 cut(s) 1003
BseJI GATNNNNATC 2 cut(s) 1002, 1146
BseLI CCNNNNNNNGG 1 cut(s) 785
BseNI ACTGG 1 cut(s) 1058
BsgI GTGCAG 1 cut(s) 78
BshFI GGCC 1 cut(s) 790
BshVI ATCGAT 2 cut(s) 711, 831
BsiSI CCGG 2 cut(s) 638, 698
BslFI GGGAC 1 cut(s) 853
BslI CCNNNNNNNGG 1 cut(s) 785
BsmAI GTCTC 1 cut(s) 323
BsmFI GGGAC 1 cut(s) 853
BsmI GAATGC 1 cut(s) 1032
BsnI GGCC 1 cut(s) 790
Bsp143I GATC 5 cut(s) 169, 560, 726, 852, 1183
BspACI CCGC 1 cut(s) 97
BspANI GGCC 1 cut(s) 790
BspDI ATCGAT 2 cut(s) 711, 831
BspLI GGNNCC 1 cut(s) 841
BspMI ACCTGC 1 cut(s) 50
BspPI GGATC 1 cut(s) 568
BsrI ACTGG 1 cut(s) 1058
BssECI CCNNGG 2 cut(s) 251, 535
BssMI GATC 5 cut(s) 169, 560, 726, 852, 1183
BssSI CACGAG 1 cut(s) 809
BssT1I CCWWGG 1 cut(s) 251
Bst2BI CACGAG 1 cut(s) 809
Bst2UI CCWGG 1 cut(s) 275
Bst4CI ACNGT 3 cut(s) 536, 979, 1079
Bst6I CTCTTC 1 cut(s) 149
BstAPI GCANNNNNTGC 2 cut(s) 65, 83
BstBAI YACGTR 1 cut(s) 286
BstDEI CTNAG 2 cut(s) 187, 730
BstDSI CCRYGG 1 cut(s) 535
BstF5I GGATG 1 cut(s) 1003
BstHHI GCGC 1 cut(s) 611
BstKTI GATC 5 cut(s) 172, 563, 729, 855, 1186
BstMAI GTCTC 1 cut(s) 323
BstMBI GATC 5 cut(s) 169, 560, 726, 852, 1183
BstMWI GCNNNNNNNGC 5 cut(s) 65, 74, 83, 972, 988
BstNI CCWGG 1 cut(s) 275
BstNSI RCATGY 2 cut(s) 90, 647
BstSCI CCNGG 2 cut(s) 273, 636
BstSNI TACGTA 1 cut(s) 286
Bsu15I ATCGAT 2 cut(s) 711, 831
BsuI GTATCC 3 cut(s) 271, 298, 472
BsuRI GGCC 1 cut(s) 790
BsuTUI ATCGAT 2 cut(s) 711, 831
BtgI CCRYGG 1 cut(s) 535
BtsCI GGATG 1 cut(s) 1003
BtsIMutI CAGTG 2 cut(s) 975, 1051
BveI ACCTGC 1 cut(s) 50
CaiI CAGNNNCTG 1 cut(s) 65
CfoI GCGC 1 cut(s) 611
Cfr13I GGNCC 3 cut(s) 62, 788, 840
ClaI ATCGAT 2 cut(s) 711, 831
Csp6I GTAC 3 cut(s) 313, 532, 641
CviAII CATG 5 cut(s) 87, 140, 526, 644, 1118
CviJI RGCY 8 cut(s) 181, 453, 636, 790, 821, 927, 966, 991
CviKI_1 RGCY 8 cut(s) 181, 453, 636, 790, 821, 927, 966, 991
CviQI GTAC 3 cut(s) 313, 532, 641
DdeI CTNAG 2 cut(s) 187, 730
DpnI GATC 5 cut(s) 171, 562, 728, 854, 1185
DpnII GATC 5 cut(s) 169, 560, 726, 852, 1183
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
Eco105I TACGTA 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 251
Eco32I GATATC 1 cut(s) 1144
Eco47I GGWCC 2 cut(s) 62, 840
EcoO109I RGGNCCY 1 cut(s) 62
EcoRI GAATTC 1 cut(s) 768
EcoRII CCWGG 1 cut(s) 273
EcoRV GATATC 1 cut(s) 1144
EcoT14I CCWWGG 1 cut(s) 251
ErhI CCWWGG 1 cut(s) 251
FaeI CATG 5 cut(s) 90, 143, 529, 647, 1121
FalI AAGNNNNNCTT 2 cut(s) 170, 202
FaqI GGGAC 1 cut(s) 853
FatI CATG 5 cut(s) 86, 139, 525, 643, 1117
FauNDI CATATG 1 cut(s) 166
FbaI TGATCA 1 cut(s) 1183
FokI GGATG 1 cut(s) 1010
FspBI CTAG 2 cut(s) 74, 252
GlaI GCGC 1 cut(s) 610
HaeIII GGCC 1 cut(s) 790
HapII CCGG 2 cut(s) 638, 698
HhaI GCGC 1 cut(s) 611
Hin1II CATG 5 cut(s) 90, 143, 529, 647, 1121
Hin6I GCGC 1 cut(s) 609
HinP1I GCGC 1 cut(s) 609
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HindIII AAGCTT 1 cut(s) 179
HpaII CCGG 2 cut(s) 638, 698
HphI GGTGA 2 cut(s) 449, 458
Hpy166II GTNNAC 3 cut(s) 206, 376, 1100
Hpy188I TCNGA 6 cut(s) 560, 914, 1047, 1063, 1166, 1192
Hpy188III TCNNGA 6 cut(s) 13, 461, 564, 573, 724, 856
Hpy8I GTNNAC 3 cut(s) 206, 376, 1100
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 5 cut(s) 304, 443, 464, 891, 893
HpyCH4III ACNGT 3 cut(s) 536, 979, 1079
HpyCH4IV ACGT 2 cut(s) 18, 285
HpyCH4V TGCA 4 cut(s) 59, 86, 236, 1124
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 74, 83, 972, 988
HpyF3I CTNAG 2 cut(s) 187, 730
HpySE526I ACGT 2 cut(s) 18, 285
Hsp92II CATG 5 cut(s) 90, 143, 529, 647, 1121
HspAI GCGC 1 cut(s) 609
Ksp22I TGATCA 1 cut(s) 1183
Kzo9I GATC 5 cut(s) 169, 560, 726, 852, 1183
LweI GCATC 2 cut(s) 934, 1111
MaeI CTAG 2 cut(s) 74, 252
MaeII ACGT 2 cut(s) 18, 285
MaeIII GTNAC 1 cut(s) 860
MalI GATC 5 cut(s) 171, 562, 728, 854, 1185
MboI GATC 5 cut(s) 169, 560, 726, 852, 1183
MboII GAAGA 3 cut(s) 143, 166, 686
MmeI TCCRAC 2 cut(s) 538, 1144
MnlI CCTC 6 cut(s) 20, 25, 34, 91, 110, 805
MroXI GAANNNNTTC 1 cut(s) 498
MseI TTAA 6 cut(s) 93, 486, 702, 933, 1014, 1198
MslI CAYNNNNRTG 1 cut(s) 321
MspI CCGG 2 cut(s) 638, 698
MspR9I CCNGG 2 cut(s) 275, 638
Mva1269I GAATGC 1 cut(s) 1032
MvaI CCWGG 1 cut(s) 275
MwoI GCNNNNNNNGC 5 cut(s) 65, 74, 83, 972, 988
NciI CCSGG 1 cut(s) 638
NdeI CATATG 1 cut(s) 166
NdeII GATC 5 cut(s) 169, 560, 726, 852, 1183
NlaIII CATG 5 cut(s) 90, 143, 529, 647, 1121
NlaIV GGNNCC 1 cut(s) 841
NmuCI GTSAC 1 cut(s) 860
NspI RCATGY 2 cut(s) 90, 647
OliI CACNNNNGTG 1 cut(s) 321
PciI ACATGT 1 cut(s) 643
PctI GAATGC 1 cut(s) 1032
PdmI GAANNNNTTC 1 cut(s) 498
Ppu21I YACGTR 1 cut(s) 286
PpuMI RGGWCCY 1 cut(s) 62
PscI ACATGT 1 cut(s) 643
Psp5II RGGWCCY 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 273
PspGI CCWGG 1 cut(s) 273
PspN4I GGNNCC 1 cut(s) 841
PspPI GGNCC 3 cut(s) 62, 788, 840
PspPPI RGGWCCY 1 cut(s) 62
PstNI CAGNNNCTG 1 cut(s) 65
RsaI GTAC 3 cut(s) 314, 533, 642
RsaNI GTAC 3 cut(s) 313, 532, 641
RseI CAYNNNNRTG 1 cut(s) 321
SaqAI TTAA 6 cut(s) 93, 486, 702, 933, 1014, 1198
Sau3AI GATC 5 cut(s) 169, 560, 726, 852, 1183
Sau96I GGNCC 3 cut(s) 62, 788, 840
ScrFI CCNGG 2 cut(s) 275, 638
SfaNI GCATC 2 cut(s) 934, 1111
SinI GGWCC 2 cut(s) 62, 840
SmiMI CAYNNNNRTG 1 cut(s) 321
SmlI CTYRAG 3 cut(s) 800, 992, 1090
SmoI CTYRAG 3 cut(s) 800, 992, 1090
SnaBI TACGTA 1 cut(s) 286
SsiI CCGC 1 cut(s) 97
SspI AATATT 1 cut(s) 954
SspMI CTAG 2 cut(s) 74, 252
StyD4I CCNGG 2 cut(s) 273, 636
StyI CCWWGG 1 cut(s) 251
TaaI ACNGT 3 cut(s) 536, 979, 1079
TaiI ACGT 2 cut(s) 21, 288
TaqI TCGA 5 cut(s) 123, 506, 711, 758, 831
Tru1I TTAA 6 cut(s) 93, 486, 702, 933, 1014, 1198
Tru9I TTAA 6 cut(s) 93, 486, 702, 933, 1014, 1198
TscAI CASTG 2 cut(s) 982, 1058
TseFI GTSAC 1 cut(s) 860
Tsp45I GTSAC 1 cut(s) 860
TspDTI ATGAA 7 cut(s) 17, 491, 531, 542, 610, 761, 921
TspRI CASTG 2 cut(s) 982, 1058
VpaK11BI GGWCC 2 cut(s) 62, 840
XapI RAATTY 5 cut(s) 568, 743, 751, 768, 1110
XceI RCATGY 2 cut(s) 90, 647
XmaJI CCTAGG 1 cut(s) 251
XmnI GAANNNNTTC 1 cut(s) 498
XspI CTAG 2 cut(s) 74, 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.