MD10G1010100.v1.1

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005259
Physical Location & Seq
Forward (+)
1386074 .. 1387630
1557 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1010100.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGGAGGAAGTGCAGGTAGTCATCGTAGGAGCCGGTCCGGCAGGCTTAGCAACCTCAGCATGTCTTAATCACCTTAAGATTCCAAATGTAGTACTCGAAAGAGAAGATTGCTATGCTTCTCTTTGGAAGAAAAAGTCATATGATCGTTTGAAGCTACACTTGGCCAAAGAATTCTGCGAACTTCCCTATATGCCCTTCCCTTCAAATGCACCCACATACATCCCTAAGGATATGTTTGTTCAATACTTGGATAATTACGTATCCCAATTCAAGATAAACCCTAAGTGCAACCGGAGTGTGAAGTCTGCTTTTTTCGATGCAAATGTAGAGAAATGGCGTGTCACAGTCGAGAACATACTTTCGGGTGAACAAGAAATATATCTTGGGAAGTTTCTTGTGGTTGCAAGTGGTGAAAATAGCGTCGGTTACATTCCTCACGTCCAAGGCTTGGACGGGTTTAAAGGGGAGGCAGTACATTCGAGCAAGTACGAAAACGGCAGGAAATATAGTGGAAAAAATGTTTTGGTAGTTGGTTCTGGGAATTCAGGCATGGAAATTGCTTATGATTTATCAAATTCGGGTGCTAATACTTCCATTGTTGTCCGTAGCCCGGTACATGTTCTCAACAAGGAAATCGTACATTTTGGAATGGTTTTGTTGAAATTCATACCACTTAAAATGGTTGACAAGGTTGTTGTGCGCTTAGGAAAATTAAAGTATGGAAATTTATCCAAGTTCGGGATTCGAAGGCCAAAAGAGGGACCTTTTTATCTCAAGGCAACTAAGGGTCGATCTGCCACTATCGATGTCTTGCCTTCCATAACAAGCATTGACGGAAACCTAATCAAATTTGCGAATGGAAACTATAACTGGTACGACGCTATTATCTTTGCTACCGGCTACAGTAGCAGTGTTCTAAACTGGTTTAAGGATGACAACGATTACTTTGATGACAATGGAATGCCACGAAAAAGTTTCCCGAATCACTGGAAATCAGAAAAGGGTCTTTACAGTGCTGGATTCTCAAGGCGTGGATTATTTGGCATTGCGGATGATGCACAATGTATAGCAAATGACATCAGTTCGTGTTTGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
FMO-like K_oxygenase Pyr_redox_3

Protein Analysis

366

Amino Acids

40.67

Weight (kDa)

8.96

Isoelectric Point (pI)

30.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMO-like PF00743 5 - 210 4.5e-19 Flavin-binding monooxygenase-like
Pyr_redox_2 PF07992 5 - 204 5e-13 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_3 PF13738 7 - 204 4.6e-22 Pyridine nucleotide-disulphide oxidoreductase
Lys_Orn_oxgnase PF13434 70 - 200 4.4e-11 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 4
AccB7I CCANNNNNTGG 1 cut(s) 448
AciI CCGC 1 cut(s) 1049
AcoI YGGCCR 1 cut(s) 162
AcsI RAATTY 6 cut(s) 170, 541, 574, 662, 724, 848
AfaI GTAC 6 cut(s) 93, 474, 488, 615, 639, 875
AfiI CCNNNNNNNGG 4 cut(s) 448, 610, 738, 758
AflII CTTAAG 1 cut(s) 74
AflIII ACRYGT 1 cut(s) 616
AgsI TTSAA 6 cut(s) 151, 204, 242, 271, 661, 1093
AjiI CACGTC 1 cut(s) 439
AjuI GAANNNNNNNTTGG 4 cut(s) 143, 175, 366, 398
AluBI AGCT 1 cut(s) 154
AluI AGCT 1 cut(s) 154
AoxI GGCC 2 cut(s) 162, 749
ApoI RAATTY 6 cut(s) 170, 541, 574, 662, 724, 848
AspLEI GCGC 1 cut(s) 702
AspS9I GGNCC 2 cut(s) 35, 761
AsuC2I CCSGG 1 cut(s) 611
AsuHPI GGTGA 3 cut(s) 62, 377, 422
AsuII TTCGAA 1 cut(s) 745
AvaII GGWCC 2 cut(s) 35, 761
AxyI CCTNAGG 1 cut(s) 225
BalI TGGCCA 1 cut(s) 164
BbvCI CCTCAGC 1 cut(s) 55
BceAI ACGGC 1 cut(s) 511
BcgI CGANNNNNNTGC 2 cut(s) 459, 493
BciVI GTATCC 1 cut(s) 271
BcnI CCSGG 1 cut(s) 611
BfaI CTAG 1 cut(s) 1096
BfmI CTRYAG 1 cut(s) 901
BfrI CTTAAG 1 cut(s) 74
BfuAI ACCTGC 1 cut(s) 4
BfuI GTATCC 1 cut(s) 271
BglI GCCNNNNNGGC 1 cut(s) 38
BlpI GCTNAGC 1 cut(s) 46
BmcAI AGTACT 1 cut(s) 93
Bme1390I CCNGG 1 cut(s) 611
Bme18I GGWCC 2 cut(s) 35, 761
BmgBI CACGTC 1 cut(s) 439
BmgT120I GGNCC 2 cut(s) 35, 761
BmiI GGNNCC 2 cut(s) 31, 762
BmrFI CCNGG 1 cut(s) 611
BmsI GCATC 2 cut(s) 307, 1045
Bpu10I CCTNAGC 2 cut(s) 55, 703
Bpu1102I GCTNAGC 1 cut(s) 46
Bpu14I TTCGAA 1 cut(s) 745
BpuEI CTTGAG 2 cut(s) 758, 1009
BpuMI CCSGG 1 cut(s) 611
Bsa29I ATCGAT 1 cut(s) 804
BsaAI YACGTR 1 cut(s) 259
BsaJI CCNNGG 1 cut(s) 442
BsaWI WCCGGW 1 cut(s) 291
Bsc4I CCNNNNNNNGG 4 cut(s) 448, 610, 738, 758
Bse118I RCCGGY 2 cut(s) 32, 896
Bse1I ACTGG 3 cut(s) 875, 926, 992
Bse21I CCTNAGG 1 cut(s) 225
Bse3DI GCAATG 1 cut(s) 1044
BseCI ATCGAT 1 cut(s) 804
BseDI CCNNGG 1 cut(s) 442
BseGI GGATG 3 cut(s) 219, 937, 1057
BseLI CCNNNNNNNGG 4 cut(s) 448, 610, 738, 758
BseMI GCAATG 1 cut(s) 1044
BseMII CTCAG 1 cut(s) 69
BseNI ACTGG 3 cut(s) 875, 926, 992
BsgI GTGCAG 1 cut(s) 32
BshFI GGCC 2 cut(s) 164, 751
BshVI ATCGAT 1 cut(s) 804
BsiSI CCGG 5 cut(s) 33, 38, 292, 611, 897
BslFI GGGAC 1 cut(s) 774
BslI CCNNNNNNNGG 4 cut(s) 448, 610, 738, 758
BsmFI GGGAC 1 cut(s) 774
BsmI GAATGC 1 cut(s) 966
BsnI GGCC 2 cut(s) 164, 751
Bsp119I TTCGAA 1 cut(s) 745
Bsp143I GATC 2 cut(s) 142, 791
Bsp1720I GCTNAGC 1 cut(s) 46
BspACI CCGC 1 cut(s) 1049
BspANI GGCC 2 cut(s) 164, 751
BspCNI CTCAG 1 cut(s) 68
BspDI ATCGAT 1 cut(s) 804
BspLI GGNNCC 2 cut(s) 31, 762
BspMI ACCTGC 1 cut(s) 4
BspT104I TTCGAA 1 cut(s) 745
BspTI CTTAAG 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 1044
BsrFI RCCGGY 2 cut(s) 32, 896
BsrI ACTGG 3 cut(s) 875, 926, 992
BssAI RCCGGY 2 cut(s) 32, 896
BssECI CCNNGG 1 cut(s) 442
BssMI GATC 2 cut(s) 142, 791
BssT1I CCWWGG 1 cut(s) 442
Bst4CI ACNGT 3 cut(s) 346, 905, 1013
BstAFI CTTAAG 1 cut(s) 74
BstBAI YACGTR 1 cut(s) 259
BstBI TTCGAA 1 cut(s) 745
BstC8I GCNNGC 1 cut(s) 43
BstDEI CTNAG 6 cut(s) 46, 55, 225, 282, 703, 783
BstF5I GGATG 3 cut(s) 219, 937, 1057
BstHHI GCGC 1 cut(s) 702
BstKTI GATC 2 cut(s) 145, 794
BstMBI GATC 2 cut(s) 142, 791
BstMWI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 906, 1055
BstNSI RCATGY 2 cut(s) 63, 620
BstSCI CCNGG 1 cut(s) 609
BstSFI CTRYAG 1 cut(s) 901
BstSNI TACGTA 1 cut(s) 259
Bsu15I ATCGAT 1 cut(s) 804
Bsu36I CCTNAGG 1 cut(s) 225
BsuI GTATCC 1 cut(s) 271
BsuRI GGCC 2 cut(s) 164, 751
BsuTUI ATCGAT 1 cut(s) 804
BtrI CACGTC 1 cut(s) 439
BtsCI GGATG 3 cut(s) 219, 937, 1057
BtsI GCAGTG 1 cut(s) 916
BtsIMutI CAGTG 3 cut(s) 916, 985, 1018
BveI ACCTGC 1 cut(s) 4
Cac8I GCNNGC 1 cut(s) 43
CfoI GCGC 1 cut(s) 702
Cfr10I RCCGGY 2 cut(s) 32, 896
Cfr13I GGNCC 2 cut(s) 35, 761
ClaI ATCGAT 1 cut(s) 804
CpoI CGGWCCG 1 cut(s) 35
CseI GACGC 2 cut(s) 409, 887
Csp6I GTAC 6 cut(s) 92, 473, 487, 614, 638, 874
CspI CGGWCCG 1 cut(s) 35
CviAII CATG 3 cut(s) 60, 550, 617
CviJI RGCY 8 cut(s) 32, 45, 154, 164, 447, 609, 751, 900
CviKI_1 RGCY 8 cut(s) 32, 45, 154, 164, 447, 609, 751, 900
CviQI GTAC 6 cut(s) 92, 473, 487, 614, 638, 874
DdeI CTNAG 6 cut(s) 46, 55, 225, 282, 703, 783
DpnI GATC 2 cut(s) 144, 793
DpnII GATC 2 cut(s) 142, 791
DraI TTTAAA 1 cut(s) 460
EaeI YGGCCR 1 cut(s) 162
Eco105I TACGTA 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 442
Eco47I GGWCC 2 cut(s) 35, 761
Eco81I CCTNAGG 1 cut(s) 225
EcoO109I RGGNCCY 1 cut(s) 761
EcoRI GAATTC 2 cut(s) 170, 541
EcoT14I CCWWGG 1 cut(s) 442
ErhI CCWWGG 1 cut(s) 442
FaeI CATG 3 cut(s) 63, 553, 620
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FaqI GGGAC 1 cut(s) 774
FatI CATG 3 cut(s) 59, 549, 616
FauNDI CATATG 1 cut(s) 139
FokI GGATG 3 cut(s) 206, 944, 1064
FspBI CTAG 1 cut(s) 1096
GlaI GCGC 1 cut(s) 701
HaeIII GGCC 2 cut(s) 164, 751
HapII CCGG 5 cut(s) 33, 38, 292, 611, 897
HgaI GACGC 2 cut(s) 409, 887
HhaI GCGC 1 cut(s) 702
Hin1II CATG 3 cut(s) 63, 553, 620
Hin6I GCGC 1 cut(s) 700
HinP1I GCGC 1 cut(s) 700
HincII GTYRAC 1 cut(s) 685
HindII GTYRAC 1 cut(s) 685
HinfI GANTC 4 cut(s) 79, 742, 982, 1020
HpaII CCGG 5 cut(s) 33, 38, 292, 611, 897
HphI GGTGA 3 cut(s) 62, 377, 422
Hpy166II GTNNAC 2 cut(s) 368, 685
Hpy188I TCNGA 1 cut(s) 997
Hpy188III TCNNGA 4 cut(s) 271, 349, 739, 979
Hpy8I GTNNAC 2 cut(s) 368, 685
Hpy99I CGWCG 2 cut(s) 425, 881
HpyAV CCTTC 4 cut(s) 205, 210, 741, 825
HpyCH4III ACNGT 3 cut(s) 346, 905, 1013
HpyCH4IV ACGT 2 cut(s) 258, 438
HpyCH4V TGCA 6 cut(s) 13, 209, 288, 320, 404, 1058
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 906, 1055
HpyF3I CTNAG 6 cut(s) 46, 55, 225, 282, 703, 783
HpySE526I ACGT 2 cut(s) 258, 438
Hsp92II CATG 3 cut(s) 63, 553, 620
HspAI GCGC 1 cut(s) 700
Kzo9I GATC 2 cut(s) 142, 791
LmnI GCTCC 1 cut(s) 29
LweI GCATC 2 cut(s) 307, 1045
MaeI CTAG 1 cut(s) 1096
MaeII ACGT 2 cut(s) 258, 438
MaeIII GTNAC 2 cut(s) 340, 425
MalI GATC 2 cut(s) 144, 793
MboI GATC 2 cut(s) 142, 791
MboII GAAGA 2 cut(s) 116, 139
MlsI TGGCCA 1 cut(s) 164
MluNI TGGCCA 1 cut(s) 164
MnlI CCTC 4 cut(s) 64, 444, 460, 751
Mox20I TGGCCA 1 cut(s) 164
MscI TGGCCA 1 cut(s) 164
MseI TTAA 6 cut(s) 66, 75, 459, 675, 713, 927
Msp20I TGGCCA 1 cut(s) 164
MspCI CTTAAG 1 cut(s) 74
MspI CCGG 5 cut(s) 33, 38, 292, 611, 897
MspR9I CCNGG 1 cut(s) 611
Mva1269I GAATGC 1 cut(s) 966
MwoI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 906, 1055
NciI CCSGG 1 cut(s) 611
NdeI CATATG 1 cut(s) 139
NdeII GATC 2 cut(s) 142, 791
NlaIII CATG 3 cut(s) 63, 553, 620
NlaIV GGNNCC 2 cut(s) 31, 762
NmuCI GTSAC 1 cut(s) 340
NspI RCATGY 2 cut(s) 63, 620
NspV TTCGAA 1 cut(s) 745
PciI ACATGT 1 cut(s) 616
PctI GAATGC 1 cut(s) 966
PfeI GAWTC 4 cut(s) 79, 742, 982, 1020
PflMI CCANNNNNTGG 1 cut(s) 448
Ppu21I YACGTR 1 cut(s) 259
PpuMI RGGWCCY 1 cut(s) 761
PscI ACATGT 1 cut(s) 616
Psp5II RGGWCCY 1 cut(s) 761
PspN4I GGNNCC 2 cut(s) 31, 762
PspPI GGNCC 2 cut(s) 35, 761
PspPPI RGGWCCY 1 cut(s) 761
RsaI GTAC 6 cut(s) 93, 474, 488, 615, 639, 875
RsaNI GTAC 6 cut(s) 92, 473, 487, 614, 638, 874
Rsr2I CGGWCCG 1 cut(s) 35
RsrII CGGWCCG 1 cut(s) 35
SaqAI TTAA 6 cut(s) 66, 75, 459, 675, 713, 927
Sau3AI GATC 2 cut(s) 142, 791
Sau96I GGNCC 2 cut(s) 35, 761
ScaI AGTACT 1 cut(s) 93
ScrFI CCNGG 1 cut(s) 611
SetI ASST 9 cut(s) 18, 56, 75, 156, 261, 441, 693, 766, 843
SfaNI GCATC 2 cut(s) 307, 1045
SfcI CTRYAG 1 cut(s) 901
SfuI TTCGAA 1 cut(s) 745
SinI GGWCC 2 cut(s) 35, 761
SmlI CTYRAG 3 cut(s) 74, 773, 1024
SmoI CTYRAG 3 cut(s) 74, 773, 1024
SnaBI TACGTA 1 cut(s) 259
SsiI CCGC 1 cut(s) 1049
SspMI CTAG 1 cut(s) 1096
StyD4I CCNGG 1 cut(s) 609
StyI CCWWGG 1 cut(s) 442
TaaI ACNGT 3 cut(s) 346, 905, 1013
TaiI ACGT 2 cut(s) 261, 441
TaqI TCGA 7 cut(s) 96, 315, 348, 479, 745, 790, 804
TatI WGTACW 2 cut(s) 91, 472
TfiI GAWTC 4 cut(s) 79, 742, 982, 1020
Tru1I TTAA 6 cut(s) 66, 75, 459, 675, 713, 927
Tru9I TTAA 6 cut(s) 66, 75, 459, 675, 713, 927
TscAI CASTG 3 cut(s) 916, 992, 1018
TseFI GTSAC 1 cut(s) 340
Tsp45I GTSAC 1 cut(s) 340
TspDTI ATGAA 1 cut(s) 655
TspGWI ACGGA 2 cut(s) 593, 849
TspRI CASTG 3 cut(s) 916, 992, 1018
Van91I CCANNNNNTGG 1 cut(s) 448
Vha464I CTTAAG 1 cut(s) 74
VpaK11BI GGWCC 2 cut(s) 35, 761
XapI RAATTY 6 cut(s) 170, 541, 574, 662, 724, 848
XceI RCATGY 2 cut(s) 63, 620
XspI CTAG 1 cut(s) 1096
ZrmI AGTACT 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.