Rroxscaffold_7G00191000

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005259
Physical Location & Seq
Reverse (-)
31457484 .. 31459583
2100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00191000.1

Sequence Viewer

Length: 873 bp
ATGCCATTCCCTAAAAATGCACCCACATATGTTCCCAGGAAAGAGTTTATTCAATACTTAGATACTTATATGTCCACTTTGAAGATAAACCCTCTGTACCACAGAAGTGTGGAGACTGCTTTCTACGATGAGGATGTGGAAAAATGGTATGTCATTGTCAACAACACAGAACTAGGTGTACAAGAAAGCTACTGCGGGAAGTTTCTTGTGGTAGCGACCGGTGAAAATAGTGAAGGATATCTTCCTCAAACGAAAGGTTTGAGTAGCTTTAAAGGCGAAATCATTCATTCGAGTGAGTATGGAAATGGCAAGAAGTACCGTGGCAAAAATATTTTAGTTGTTGGATCTGGGAATTCTGGAATGGAAATTGCCTATGACTTATCAAATTTCGGGGCCAATACTTCTATTGTTGTCCGTAGCCCGGTACATGTTCTTACCAAGGAGATCGTATTCATTGGAATGGTTCTGTTAAAGTACCTTCCCGTTAAGGTTGTAGACGGCATTGTCATGATCTTAACCAAGTGGAAATTTGGAAATTTATCCAAGTATGGGATTCAAATTCCAAGAATGGGGCCATTTTATACCAAGGAAAACAAAGGTCAATCCCCCATCATCGATGTTGGGACTATTGAAAAGATCAAGAGCGGAGAAATACAGGTTTTGCCGTCCATAACAAGTATACAAGGAAATGAAATTTGGTTTGAGAGTGGCTACCTTAAATGTTATGATGCTATAGTATTTGCTACAGGCTATAAAAGCACTGTTCTAAAGTGGCTCAAGGATGAAAACAACCTCTTTAGTGACAATGGAATGCCGAAGAAAAGTTTCCCCAACCACTGGAAGTCACAAAATGGCCTTTTCTGTGGGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
FMO-like K_oxygenase Pyr_redox_3

Protein Analysis

290

Amino Acids

32.61

Weight (kDa)

8.83

Isoelectric Point (pI)

34.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMO-like PF00743 2 - 253 2.3e-19 Flavin-binding monooxygenase-like
Lys_Orn_oxgnase PF13434 6 - 136 2e-14 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Pyr_redox_3 PF13738 9 - 141 1.5e-11 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_2 PF07992 47 - 141 1.3e-09 Pyridine nucleotide-disulphide oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 503
AccB7I CCANNNNNTGG 1 cut(s) 837
AccBSI CCGCTC 1 cut(s) 645
AccI GTMKAC 2 cut(s) 495, 679
AciI CCGC 2 cut(s) 195, 645
AclWI GGATC 1 cut(s) 352
AcsI RAATTY 6 cut(s) 352, 385, 527, 535, 558, 693
AfaI GTAC 5 cut(s) 98, 180, 317, 426, 476
AfiI CCNNNNNNNGG 4 cut(s) 421, 549, 569, 837
AflIII ACRYGT 1 cut(s) 427
AgeI ACCGGT 1 cut(s) 218
AgsI TTSAA 4 cut(s) 53, 82, 557, 632
AjnI CCWGG 1 cut(s) 35
AjuI GAANNNNNNNTTGG 2 cut(s) 679, 711
AleI CACNNNNGTG 1 cut(s) 105
AluBI AGCT 2 cut(s) 189, 267
AluI AGCT 2 cut(s) 189, 267
Alw26I GTCTC 1 cut(s) 107
AlwI GGATC 1 cut(s) 352
AoxI GGCC 4 cut(s) 393, 572, 853, 866
ApoI RAATTY 6 cut(s) 352, 385, 527, 535, 558, 693
AsiGI ACCGGT 1 cut(s) 218
Asp700I GAANNNNTTC 2 cut(s) 282, 824
AspS9I GGNCC 3 cut(s) 393, 572, 866
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 1 cut(s) 233
BccI CCATC 1 cut(s) 617
BceAI ACGGC 2 cut(s) 514, 649
BciT130I CCWGG 1 cut(s) 37
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 1 cut(s) 107
BfaI CTAG 1 cut(s) 173
BfmI CTRYAG 2 cut(s) 732, 744
Bme1390I CCNGG 2 cut(s) 37, 422
BmgT120I GGNCC 3 cut(s) 393, 572, 866
BmiI GGNNCC 2 cut(s) 394, 573
BmrFI CCNGG 2 cut(s) 37, 422
BmsI GCATC 1 cut(s) 718
BpuEI CTTGAG 1 cut(s) 761
BpuMI CCSGG 1 cut(s) 422
Bsa29I ATCGAT 1 cut(s) 615
BsaJI CCNNGG 4 cut(s) 35, 319, 438, 585
BsaWI WCCGGW 1 cut(s) 218
Bsc4I CCNNNNNNNGG 4 cut(s) 421, 549, 569, 837
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 1 cut(s) 842
BseBI CCWGG 1 cut(s) 37
BseCI ATCGAT 1 cut(s) 615
BseDI CCNNGG 4 cut(s) 35, 319, 438, 585
BseGI GGATG 2 cut(s) 139, 787
BseLI CCNNNNNNNGG 4 cut(s) 421, 549, 569, 837
BseNI ACTGG 1 cut(s) 842
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 4 cut(s) 395, 574, 855, 868
BshTI ACCGGT 1 cut(s) 218
BshVI ATCGAT 1 cut(s) 615
BsiEI CGRYCG 1 cut(s) 219
BsiSI CCGG 2 cut(s) 219, 422
BslFI GGGAC 1 cut(s) 637
BslI CCNNNNNNNGG 4 cut(s) 421, 549, 569, 837
BsmAI GTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 637
BsmI GAATGC 1 cut(s) 816
BsnI GGCC 4 cut(s) 395, 574, 855, 868
Bsp1407I TGTACA 1 cut(s) 178
Bsp143I GATC 4 cut(s) 344, 444, 510, 636
BspACI CCGC 2 cut(s) 195, 645
BspANI GGCC 4 cut(s) 395, 574, 855, 868
BspDI ATCGAT 1 cut(s) 615
BspHI TCATGA 1 cut(s) 507
BspLI GGNNCC 2 cut(s) 394, 573
BspPI GGATC 1 cut(s) 352
BsrBI CCGCTC 1 cut(s) 645
BsrFI RCCGGY 1 cut(s) 218
BsrGI TGTACA 1 cut(s) 178
BsrI ACTGG 1 cut(s) 842
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 4 cut(s) 35, 319, 438, 585
BssMI GATC 4 cut(s) 344, 444, 510, 636
BssNAI GTATAC 1 cut(s) 680
BssT1I CCWWGG 2 cut(s) 438, 585
Bst1107I GTATAC 1 cut(s) 680
Bst2UI CCWGG 1 cut(s) 37
Bst4CI ACNGT 2 cut(s) 320, 763
BstAUI TGTACA 1 cut(s) 178
BstDEI CTNAG 1 cut(s) 58
BstDSI CCRYGG 1 cut(s) 319
BstF5I GGATG 2 cut(s) 139, 787
BstKTI GATC 4 cut(s) 347, 447, 513, 639
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 4 cut(s) 344, 444, 510, 636
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 2 cut(s) 273, 756
BstNI CCWGG 1 cut(s) 37
BstNSI RCATGY 1 cut(s) 431
BstSCI CCNGG 2 cut(s) 35, 420
BstSFI CTRYAG 2 cut(s) 732, 744
BstX2I RGATCY 1 cut(s) 344
BstYI RGATCY 1 cut(s) 344
BstZ17I GTATAC 1 cut(s) 680
Bsu15I ATCGAT 1 cut(s) 615
BsuRI GGCC 4 cut(s) 395, 574, 855, 868
BsuTUI ATCGAT 1 cut(s) 615
BtgI CCRYGG 1 cut(s) 319
BtsCI GGATG 2 cut(s) 139, 787
BtsIMutI CAGTG 2 cut(s) 759, 835
CciI TCATGA 1 cut(s) 507
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 3 cut(s) 393, 572, 866
ClaI ATCGAT 1 cut(s) 615
Csp6I GTAC 5 cut(s) 97, 179, 316, 425, 475
CspAI ACCGGT 1 cut(s) 218
CviAII CATG 2 cut(s) 428, 508
CviQI GTAC 5 cut(s) 97, 179, 316, 425, 475
DdeI CTNAG 1 cut(s) 58
DpnI GATC 4 cut(s) 346, 446, 512, 638
DpnII GATC 4 cut(s) 344, 444, 510, 636
DraI TTTAAA 1 cut(s) 271
DrdI GACNNNNNNGTC 1 cut(s) 503
DseDI GACNNNNNNGTC 1 cut(s) 503
Eco130I CCWWGG 2 cut(s) 438, 585
Eco32I GATATC 1 cut(s) 239
EcoRI GAATTC 1 cut(s) 352
EcoRII CCWGG 1 cut(s) 35
EcoRV GATATC 1 cut(s) 239
EcoT14I CCWWGG 2 cut(s) 438, 585
ErhI CCWWGG 2 cut(s) 438, 585
FaeI CATG 2 cut(s) 431, 511
FalI AAGNNNNNCTT 2 cut(s) 225, 257
FaqI GGGAC 1 cut(s) 637
FatI CATG 2 cut(s) 427, 507
FauI CCCGC 1 cut(s) 188
FauNDI CATATG 1 cut(s) 28
FblI GTMKAC 2 cut(s) 495, 679
FokI GGATG 2 cut(s) 146, 794
FspBI CTAG 1 cut(s) 173
HaeIII GGCC 4 cut(s) 395, 574, 855, 868
HapII CCGG 2 cut(s) 219, 422
Hin1II CATG 2 cut(s) 431, 511
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 1 cut(s) 553
HpaII CCGG 2 cut(s) 219, 422
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 5 cut(s) 75, 160, 179, 496, 680
Hpy188III TCNNGA 3 cut(s) 357, 508, 640
Hpy8I GTNNAC 5 cut(s) 75, 160, 179, 496, 680
HpyAV CCTTC 2 cut(s) 227, 488
HpyCH4III ACNGT 2 cut(s) 320, 763
HpyCH4V TGCA 1 cut(s) 20
HpyF10VI GCNNNNNNNGC 2 cut(s) 273, 756
HpyF3I CTNAG 1 cut(s) 58
Hsp92II CATG 2 cut(s) 431, 511
Kzo9I GATC 4 cut(s) 344, 444, 510, 636
LpnPI CCDG 9 cut(s) 22, 49, 232, 333, 342, 435, 641, 732, 823
LweI GCATC 1 cut(s) 718
MaeI CTAG 1 cut(s) 173
MaeIII GTNAC 2 cut(s) 800, 843
MalI GATC 4 cut(s) 346, 446, 512, 638
MbiI CCGCTC 1 cut(s) 645
MboI GATC 4 cut(s) 344, 444, 510, 636
MboII GAAGA 3 cut(s) 94, 233, 829
MflI RGATCY 1 cut(s) 344
MluCI AATT 7 cut(s) 352, 366, 385, 527, 535, 558, 693
MmeI TCCRAC 1 cut(s) 322
MnlI CCTC 4 cut(s) 102, 124, 255, 803
MroXI GAANNNNTTC 2 cut(s) 282, 824
MseI TTAA 5 cut(s) 270, 470, 486, 515, 717
MslI CAYNNNNRTG 4 cut(s) 105, 291, 458, 506
MspI CCGG 2 cut(s) 219, 422
MspR9I CCNGG 2 cut(s) 37, 422
Mva1269I GAATGC 1 cut(s) 816
MvaI CCWGG 1 cut(s) 37
MwoI GCNNNNNNNGC 2 cut(s) 273, 756
NciI CCSGG 1 cut(s) 422
NdeI CATATG 1 cut(s) 28
NdeII GATC 4 cut(s) 344, 444, 510, 636
NlaIII CATG 2 cut(s) 431, 511
NlaIV GGNNCC 2 cut(s) 394, 573
NmuCI GTSAC 2 cut(s) 800, 843
NspI RCATGY 1 cut(s) 431
OliI CACNNNNGTG 1 cut(s) 105
PagI TCATGA 1 cut(s) 507
PciI ACATGT 1 cut(s) 427
PctI GAATGC 1 cut(s) 816
PdmI GAANNNNTTC 2 cut(s) 282, 824
PfeI GAWTC 1 cut(s) 553
PflMI CCANNNNNTGG 1 cut(s) 837
PinAI ACCGGT 1 cut(s) 218
PscI ACATGT 1 cut(s) 427
Psp6I CCWGG 1 cut(s) 35
PspGI CCWGG 1 cut(s) 35
PspN4I GGNNCC 2 cut(s) 394, 573
PspPI GGNCC 3 cut(s) 393, 572, 866
PsrI GAACNNNNNNTAC 2 cut(s) 162, 194
PsuI RGATCY 1 cut(s) 344
RsaI GTAC 5 cut(s) 98, 180, 317, 426, 476
RsaNI GTAC 5 cut(s) 97, 179, 316, 425, 475
RseI CAYNNNNRTG 4 cut(s) 105, 291, 458, 506
SaqAI TTAA 5 cut(s) 270, 470, 486, 515, 717
Sau3AI GATC 4 cut(s) 344, 444, 510, 636
Sau96I GGNCC 3 cut(s) 393, 572, 866
ScrFI CCNGG 2 cut(s) 37, 422
SfaNI GCATC 1 cut(s) 718
SfcI CTRYAG 2 cut(s) 732, 744
SmiMI CAYNNNNRTG 4 cut(s) 105, 291, 458, 506
SmlI CTYRAG 1 cut(s) 776
SmoI CTYRAG 1 cut(s) 776
Sse9I AATT 7 cut(s) 352, 366, 385, 527, 535, 558, 693
SsiI CCGC 2 cut(s) 195, 645
SspI AATATT 1 cut(s) 331
SspMI CTAG 1 cut(s) 173
StyD4I CCNGG 2 cut(s) 35, 420
StyI CCWWGG 2 cut(s) 438, 585
TaaI ACNGT 2 cut(s) 320, 763
TaqI TCGA 2 cut(s) 290, 615
TasI AATT 7 cut(s) 352, 366, 385, 527, 535, 558, 693
TatI WGTACW 1 cut(s) 178
TfiI GAWTC 1 cut(s) 553
Tru1I TTAA 5 cut(s) 270, 470, 486, 515, 717
Tru9I TTAA 5 cut(s) 270, 470, 486, 515, 717
TscAI CASTG 2 cut(s) 766, 842
TseFI GTSAC 2 cut(s) 800, 843
Tsp45I GTSAC 2 cut(s) 800, 843
TspDTI ATGAA 4 cut(s) 275, 442, 705, 798
TspGWI ACGGA 1 cut(s) 404
TspRI CASTG 2 cut(s) 766, 842
Van91I CCANNNNNTGG 1 cut(s) 837
XapI RAATTY 6 cut(s) 352, 385, 527, 535, 558, 693
XceI RCATGY 1 cut(s) 431
XmiI GTMKAC 2 cut(s) 495, 679
XmnI GAANNNNTTC 2 cut(s) 282, 824
XspI CTAG 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.