MD10G1010000.v1.1

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005259
Physical Location & Seq
Forward (+)
1378296 .. 1380232
1937 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1010000.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGGACGAAGTACAAGTGATCATCGTAGGTGCCGGTCCGGCCGGCTTAGCAACCTCAGCATGTCTTAATCACCTTAAGATTTCAAATGTAGTACTCGAAAGAGAAGATTGCTATGCTTCTCTTTGGAAGAAAAGGTCATATGATCGTTTGAAGCTACACTTGGCCAAAGAATTCTGCGAACTTCCCTATATGACTTTCCCTCCAAATGCACCCACATACATCCCCAAGGATATGTTTGTTCAATACTTGGAAACTAATGTATCCCAACTCGGAATAAACCCTAGGTGCAACCAAAACGTGAAGTCTGCTCTTTATGAAGTAGACATAAACAAATGGCGTGTCACGGCCGAGAACACGCTTTTGGGTGCACAAGAAGAGTACCTTGGGAAGTTTCTTGTGGTTGCAAGTGGTGAAAATAGCATAGGTTATGTTCCTGAAGTGCAAGGCTTGGATGAGTTCAAAGGGGAGGCCATTCATTCAAGCAACTACGAAAACGGCAAGAAATATGGTGGAAAAAATGTTTTAGTCGTTGGTTCTGGGAATTCTGGCATGGAAATTGCATATGATTTATCAAATTCGGGTGCTAATACGTCCATTGTTATCCGTAGCCCGGTACATGTTCTCAACAAGGAAATTGTACATTTCGGAATGGTTTCGTTAAAATTCCTCCCACTTAACATAGTCGACAAGGCTGTTGTGCTCTTAGGAAAACTAAAATATGGAGATTTAACGAAGTATGGGATTCGAAAGCCAAAAGAGGGACCTTTTTTTCTCAAGGCAGCTAAGGGTCGTGCCCCCACTATCGATGTTGGGTCCATTAACAAGATTAAAACTGGAGAGATAAAGGTCTTGCCATCCATAACAAGCATAGACGGAGACCTGATCAGATTTCAGAACGGCAACGTTGATTCGTACAATGCTATTATATTTGCTACAGGCTACAAAAGCAGCGTTCTAAACTGGCTCCAGGATGACAACCATTACTTCAACGAGAATGGAATGCCACAAAAAAGTTTCCCGAATCACTGGAAATCGGAAAAAAAGGGTCTTTACAGTGCTGGGTTCTCAAGACGTGGGTTATTTGGCATTGCATATGACGCACGAAGTATAGCAGAAGACATCAGTTCGTGTTTGACCTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
FMO-like K_oxygenase Pyr_redox_3

Protein Analysis

381

Amino Acids

41.98

Weight (kDa)

8.56

Isoelectric Point (pI)

30.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMO-like PF00743 5 - 317 1.4e-18 Flavin-binding monooxygenase-like
Pyr_redox_2 PF07992 5 - 204 1.4e-13 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_3 PF13738 7 - 204 3.5e-21 Pyridine nucleotide-disulphide oxidoreductase
Lys_Orn_oxgnase PF13434 72 - 200 1.9e-10 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 29
AccI GTMKAC 2 cut(s) 321, 684
AclI AACGTT 1 cut(s) 903
AcoI YGGCCR 3 cut(s) 39, 162, 345
AcsI RAATTY 4 cut(s) 170, 541, 574, 662
AcuI CTGAAG 1 cut(s) 456
AfaI GTAC 6 cut(s) 12, 93, 380, 615, 639, 914
AfiI CCNNNNNNNGG 2 cut(s) 610, 758
AflII CTTAAG 1 cut(s) 74
AflIII ACRYGT 1 cut(s) 616
AgsI TTSAA 6 cut(s) 84, 151, 242, 460, 480, 988
AjiI CACGTC 1 cut(s) 1073
AjnI CCWGG 1 cut(s) 966
AjuI GAANNNNNNNTTGG 6 cut(s) 143, 175, 344, 366, 376, 398
AluBI AGCT 2 cut(s) 154, 782
AluI AGCT 2 cut(s) 154, 782
Alw21I GWGCWC 2 cut(s) 370, 702
Alw26I GTCTC 1 cut(s) 870
Alw44I GTGCAC 1 cut(s) 366
AoxI GGCC 4 cut(s) 39, 162, 345, 468
ApaLI GTGCAC 1 cut(s) 366
ApeKI GCWGC 2 cut(s) 779, 948
ApoI RAATTY 4 cut(s) 170, 541, 574, 662
ArsI GACNNNNNNTTYG 2 cut(s) 324, 356
Asp700I GAANNNNTTC 1 cut(s) 652
AspA2I CCTAGG 1 cut(s) 281
AspS9I GGNCC 3 cut(s) 35, 761, 813
AsuC2I CCSGG 1 cut(s) 611
AsuHPI GGTGA 2 cut(s) 62, 422
AsuII TTCGAA 1 cut(s) 745
AvaII GGWCC 3 cut(s) 35, 761, 813
AvrII CCTAGG 1 cut(s) 281
BaeGI GKGCMC 2 cut(s) 370, 796
BaeI ACNNNNGTAYC 2 cut(s) 243, 276
BalI TGGCCA 1 cut(s) 164
BanI GGYRCC 1 cut(s) 29
BbsI GAAGAC 1 cut(s) 1122
Bbv12I GWGCWC 2 cut(s) 370, 702
BbvCI CCTCAGC 1 cut(s) 55
BbvI GCAGC 2 cut(s) 791, 960
BccI CCATC 1 cut(s) 862
BceAI ACGGC 3 cut(s) 360, 511, 913
BciT130I CCWGG 1 cut(s) 968
BciVI GTATCC 1 cut(s) 271
BclI TGATCA 2 cut(s) 18, 882
BcnI CCSGG 1 cut(s) 611
BcoDI GTCTC 1 cut(s) 870
BfaI CTAG 1 cut(s) 282
BfmI CTRYAG 1 cut(s) 933
BfrI CTTAAG 1 cut(s) 74
BfuI GTATCC 1 cut(s) 271
BglI GCCNNNNNGGC 1 cut(s) 38
BisI GCNGC 2 cut(s) 780, 949
BlnI CCTAGG 1 cut(s) 281
BlpI GCTNAGC 1 cut(s) 46
BlsI GCNGC 2 cut(s) 781, 950
BmcAI AGTACT 1 cut(s) 93
Bme1390I CCNGG 2 cut(s) 611, 968
Bme18I GGWCC 3 cut(s) 35, 761, 813
BmgBI CACGTC 1 cut(s) 1073
BmgT120I GGNCC 3 cut(s) 35, 761, 813
BmiI GGNNCC 4 cut(s) 31, 762, 814, 965
BmrFI CCNGG 2 cut(s) 611, 968
BpiI GAAGAC 1 cut(s) 1122
BpmI CTGGAG 2 cut(s) 855, 950
Bpu10I CCTNAGC 2 cut(s) 55, 783
Bpu1102I GCTNAGC 1 cut(s) 46
Bpu14I TTCGAA 1 cut(s) 745
BpuEI CTTGAG 2 cut(s) 758, 1051
BpuMI CCSGG 1 cut(s) 611
Bsa29I ATCGAT 1 cut(s) 804
BsaI GGTCTC 1 cut(s) 870
BsaJI CCNNGG 3 cut(s) 225, 281, 382
BsaXI ACNNNNNCTCC 2 cut(s) 184, 214
Bsc4I CCNNNNNNNGG 2 cut(s) 610, 758
Bse118I RCCGGY 2 cut(s) 32, 41
Bse1I ACTGG 3 cut(s) 838, 965, 1031
Bse3DI GCAATG 1 cut(s) 1086
BseBI CCWGG 1 cut(s) 968
BseCI ATCGAT 1 cut(s) 804
BseDI CCNNGG 3 cut(s) 225, 281, 382
BseGI GGATG 4 cut(s) 219, 457, 854, 976
BseLI CCNNNNNNNGG 2 cut(s) 610, 758
BseMI GCAATG 1 cut(s) 1086
BseMII CTCAG 1 cut(s) 69
BseNI ACTGG 3 cut(s) 838, 965, 1031
BseSI GKGCMC 2 cut(s) 370, 796
BseX3I CGGCCG 2 cut(s) 39, 345
BseXI GCAGC 2 cut(s) 791, 960
BseYI CCCAGC 1 cut(s) 1058
Bsh1285I CGRYCG 2 cut(s) 42, 348
BshFI GGCC 4 cut(s) 41, 164, 347, 470
BshNI GGYRCC 1 cut(s) 29
BshVI ATCGAT 1 cut(s) 804
BsiEI CGRYCG 2 cut(s) 42, 348
BsiHKAI GWGCWC 2 cut(s) 370, 702
BsiSI CCGG 4 cut(s) 33, 38, 42, 611
BslFI GGGAC 1 cut(s) 774
BslI CCNNNNNNNGG 2 cut(s) 610, 758
BsmAI GTCTC 1 cut(s) 870
BsmFI GGGAC 1 cut(s) 774
BsmI GAATGC 1 cut(s) 1005
BsnI GGCC 4 cut(s) 41, 164, 347, 470
Bso31I GGTCTC 1 cut(s) 870
Bsp119I TTCGAA 1 cut(s) 745
Bsp1286I GDGCHC 3 cut(s) 370, 702, 796
Bsp1407I TGTACA 1 cut(s) 637
Bsp143I GATC 3 cut(s) 18, 142, 882
Bsp1720I GCTNAGC 1 cut(s) 46
BspANI GGCC 4 cut(s) 41, 164, 347, 470
BspCNI CTCAG 1 cut(s) 68
BspDI ATCGAT 1 cut(s) 804
BspLI GGNNCC 4 cut(s) 31, 762, 814, 965
BspT104I TTCGAA 1 cut(s) 745
BspT107I GGYRCC 1 cut(s) 29
BspTI CTTAAG 1 cut(s) 74
BspTNI GGTCTC 1 cut(s) 870
BsrDI GCAATG 1 cut(s) 1086
BsrFI RCCGGY 2 cut(s) 32, 41
BsrGI TGTACA 1 cut(s) 637
BsrI ACTGG 3 cut(s) 838, 965, 1031
BssAI RCCGGY 2 cut(s) 32, 41
BssECI CCNNGG 3 cut(s) 225, 281, 382
BssMI GATC 3 cut(s) 18, 142, 882
BssT1I CCWWGG 3 cut(s) 225, 281, 382
Bst2UI CCWGG 1 cut(s) 968
Bst4CI ACNGT 1 cut(s) 1055
Bst6I CTCTTC 1 cut(s) 369
BstAFI CTTAAG 1 cut(s) 74
BstAUI TGTACA 1 cut(s) 637
BstBI TTCGAA 1 cut(s) 745
BstC8I GCNNGC 1 cut(s) 43
BstDEI CTNAG 4 cut(s) 46, 55, 703, 783
BstF5I GGATG 4 cut(s) 219, 457, 854, 976
BstKTI GATC 3 cut(s) 21, 145, 885
BstMAI GTCTC 1 cut(s) 870
BstMBI GATC 3 cut(s) 18, 142, 882
BstMCI CGRYCG 2 cut(s) 42, 348
BstMWI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 945, 1097
BstNI CCWGG 1 cut(s) 968
BstNSI RCATGY 2 cut(s) 63, 620
BstSCI CCNGG 2 cut(s) 609, 966
BstSFI CTRYAG 1 cut(s) 933
BstSLI GKGCMC 2 cut(s) 370, 796
BstV1I GCAGC 2 cut(s) 791, 960
BstV2I GAAGAC 1 cut(s) 1122
BstZI CGGCCG 2 cut(s) 39, 345
Bsu15I ATCGAT 1 cut(s) 804
BsuI GTATCC 1 cut(s) 271
BsuRI GGCC 4 cut(s) 41, 164, 347, 470
BsuTUI ATCGAT 1 cut(s) 804
BtrI CACGTC 1 cut(s) 1073
BtsCI GGATG 4 cut(s) 219, 457, 854, 976
BtsIMutI CAGTG 2 cut(s) 1024, 1060
Cac8I GCNNGC 1 cut(s) 43
Cfr10I RCCGGY 2 cut(s) 32, 41
Cfr13I GGNCC 3 cut(s) 35, 761, 813
ClaI ATCGAT 1 cut(s) 804
CpoI CGGWCCG 1 cut(s) 35
CseI GACGC 1 cut(s) 1106
Csp6I GTAC 6 cut(s) 11, 92, 379, 614, 638, 913
CspI CGGWCCG 1 cut(s) 35
CviAII CATG 3 cut(s) 60, 550, 617
CviQI GTAC 6 cut(s) 11, 92, 379, 614, 638, 913
DdeI CTNAG 4 cut(s) 46, 55, 703, 783
DpnI GATC 3 cut(s) 20, 144, 884
DpnII GATC 3 cut(s) 18, 142, 882
EaeI YGGCCR 3 cut(s) 39, 162, 345
EagI CGGCCG 2 cut(s) 39, 345
Eam1104I CTCTTC 1 cut(s) 369
EarI CTCTTC 1 cut(s) 369
EclXI CGGCCG 2 cut(s) 39, 345
Eco130I CCWWGG 3 cut(s) 225, 281, 382
Eco31I GGTCTC 1 cut(s) 870
Eco47I GGWCC 3 cut(s) 35, 761, 813
Eco52I CGGCCG 2 cut(s) 39, 345
Eco57I CTGAAG 1 cut(s) 456
EcoO109I RGGNCCY 1 cut(s) 761
EcoRI GAATTC 2 cut(s) 170, 541
EcoRII CCWGG 1 cut(s) 966
EcoT14I CCWWGG 3 cut(s) 225, 281, 382
ErhI CCWWGG 3 cut(s) 225, 281, 382
FaeI CATG 3 cut(s) 63, 553, 620
FalI AAGNNNNNCTT 4 cut(s) 143, 175, 366, 398
FaqI GGGAC 1 cut(s) 774
FatI CATG 3 cut(s) 59, 549, 616
FauNDI CATATG 3 cut(s) 139, 562, 1093
FbaI TGATCA 2 cut(s) 18, 882
FblI GTMKAC 2 cut(s) 321, 684
Fnu4HI GCNGC 2 cut(s) 780, 949
FokI GGATG 4 cut(s) 206, 464, 841, 983
Fsp4HI GCNGC 2 cut(s) 780, 949
FspBI CTAG 1 cut(s) 282
GluI GCNGC 2 cut(s) 780, 949
GsaI CCCAGC 1 cut(s) 1062
GsuI CTGGAG 2 cut(s) 855, 950
HaeIII GGCC 4 cut(s) 41, 164, 347, 470
HapII CCGG 4 cut(s) 33, 38, 42, 611
HgaI GACGC 1 cut(s) 1106
Hin1II CATG 3 cut(s) 63, 553, 620
HincII GTYRAC 1 cut(s) 685
HindII GTYRAC 1 cut(s) 685
HinfI GANTC 3 cut(s) 742, 908, 1021
HpaII CCGG 4 cut(s) 33, 38, 42, 611
HphI GGTGA 2 cut(s) 62, 422
Hpy166II GTNNAC 3 cut(s) 322, 368, 685
Hpy188I TCNGA 5 cut(s) 272, 647, 887, 894, 1036
Hpy188III TCNNGA 4 cut(s) 434, 1018, 1068, 1140
Hpy8I GTNNAC 3 cut(s) 322, 368, 685
HpyCH4III ACNGT 1 cut(s) 1055
HpyCH4IV ACGT 4 cut(s) 297, 590, 903, 1072
HpyCH4V TGCA 7 cut(s) 209, 288, 368, 404, 442, 560, 1091
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 945, 1097
HpyF3I CTNAG 4 cut(s) 46, 55, 703, 783
HpySE526I ACGT 4 cut(s) 297, 590, 903, 1072
Hsp92II CATG 3 cut(s) 63, 553, 620
KroI GCCGGC 1 cut(s) 41
KroNI GCCGGC 1 cut(s) 43
Ksp22I TGATCA 2 cut(s) 18, 882
Kzo9I GATC 3 cut(s) 18, 142, 882
LmnI GCTCC 1 cut(s) 969
Lsp1109I GCAGC 2 cut(s) 791, 960
MaeI CTAG 1 cut(s) 282
MaeII ACGT 4 cut(s) 297, 590, 903, 1072
MaeIII GTNAC 1 cut(s) 340
MalI GATC 3 cut(s) 20, 144, 884
MboI GATC 3 cut(s) 18, 142, 882
MboII GAAGA 4 cut(s) 116, 139, 386, 1127
MhlI GDGCHC 3 cut(s) 370, 702, 796
MlsI TGGCCA 1 cut(s) 164
MluCI AATT 6 cut(s) 170, 541, 555, 574, 633, 662
MluNI TGGCCA 1 cut(s) 164
MnlI CCTC 5 cut(s) 64, 210, 460, 677, 751
Mox20I TGGCCA 1 cut(s) 164
MroNI GCCGGC 1 cut(s) 41
MroXI GAANNNNTTC 1 cut(s) 652
MscI TGGCCA 1 cut(s) 164
MseI TTAA 7 cut(s) 66, 75, 659, 675, 728, 819, 828
Msp20I TGGCCA 1 cut(s) 164
MspCI CTTAAG 1 cut(s) 74
MspI CCGG 4 cut(s) 33, 38, 42, 611
MspR9I CCNGG 2 cut(s) 611, 968
Mva1269I GAATGC 1 cut(s) 1005
MvaI CCWGG 1 cut(s) 968
MwoI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 945, 1097
NaeI GCCGGC 1 cut(s) 43
NciI CCSGG 1 cut(s) 611
NdeI CATATG 3 cut(s) 139, 562, 1093
NdeII GATC 3 cut(s) 18, 142, 882
NgoMIV GCCGGC 1 cut(s) 41
NlaIII CATG 3 cut(s) 63, 553, 620
NlaIV GGNNCC 4 cut(s) 31, 762, 814, 965
NmeAIII GCCGAG 1 cut(s) 373
NmuCI GTSAC 1 cut(s) 340
NspI RCATGY 2 cut(s) 63, 620
NspV TTCGAA 1 cut(s) 745
PciI ACATGT 1 cut(s) 616
PctI GAATGC 1 cut(s) 1005
PdiI GCCGGC 1 cut(s) 43
PdmI GAANNNNTTC 1 cut(s) 652
PfeI GAWTC 3 cut(s) 742, 908, 1021
PfoI TCCNGGA 1 cut(s) 966
PkrI GCNGC 2 cut(s) 781, 950
PpuMI RGGWCCY 1 cut(s) 761
PscI ACATGT 1 cut(s) 616
Psp1406I AACGTT 1 cut(s) 903
Psp5II RGGWCCY 1 cut(s) 761
Psp6I CCWGG 1 cut(s) 966
PspFI CCCAGC 1 cut(s) 1058
PspGI CCWGG 1 cut(s) 966
PspN4I GGNNCC 4 cut(s) 31, 762, 814, 965
PspPI GGNCC 3 cut(s) 35, 761, 813
PspPPI RGGWCCY 1 cut(s) 761
RsaI GTAC 6 cut(s) 12, 93, 380, 615, 639, 914
RsaNI GTAC 6 cut(s) 11, 92, 379, 614, 638, 913
Rsr2I CGGWCCG 1 cut(s) 35
RsrII CGGWCCG 1 cut(s) 35
SalI GTCGAC 1 cut(s) 683
SaqAI TTAA 7 cut(s) 66, 75, 659, 675, 728, 819, 828
SatI GCNGC 2 cut(s) 780, 949
Sau3AI GATC 3 cut(s) 18, 142, 882
Sau96I GGNCC 3 cut(s) 35, 761, 813
ScaI AGTACT 1 cut(s) 93
ScrFI CCNGG 2 cut(s) 611, 968
SduI GDGCHC 3 cut(s) 370, 702, 796
SfcI CTRYAG 1 cut(s) 933
SfuI TTCGAA 1 cut(s) 745
SinI GGWCC 3 cut(s) 35, 761, 813
SmlI CTYRAG 3 cut(s) 74, 773, 1066
SmoI CTYRAG 3 cut(s) 74, 773, 1066
Sse9I AATT 6 cut(s) 170, 541, 555, 574, 633, 662
SspMI CTAG 1 cut(s) 282
StyD4I CCNGG 2 cut(s) 609, 966
StyI CCWWGG 3 cut(s) 225, 281, 382
TaaI ACNGT 1 cut(s) 1055
TaiI ACGT 4 cut(s) 300, 593, 906, 1075
TaqI TCGA 4 cut(s) 96, 684, 745, 804
TasI AATT 6 cut(s) 170, 541, 555, 574, 633, 662
TatI WGTACW 3 cut(s) 10, 91, 637
TfiI GAWTC 3 cut(s) 742, 908, 1021
Tru1I TTAA 7 cut(s) 66, 75, 659, 675, 728, 819, 828
Tru9I TTAA 7 cut(s) 66, 75, 659, 675, 728, 819, 828
TscAI CASTG 2 cut(s) 1031, 1060
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 2 cut(s) 779, 948
Tsp45I GTSAC 1 cut(s) 340
TspDTI ATGAA 2 cut(s) 330, 464
TspGWI ACGGA 2 cut(s) 593, 888
TspRI CASTG 2 cut(s) 1031, 1060
Vha464I CTTAAG 1 cut(s) 74
VneI GTGCAC 1 cut(s) 366
VpaK11BI GGWCC 3 cut(s) 35, 761, 813
XapI RAATTY 4 cut(s) 170, 541, 574, 662
XceI RCATGY 2 cut(s) 63, 620
XmaJI CCTAGG 1 cut(s) 281
XmiI GTMKAC 2 cut(s) 321, 684
XmnI GAANNNNTTC 1 cut(s) 652
XspI CTAG 1 cut(s) 282
ZrmI AGTACT 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.