Rroxscaffold_6G00397340

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005259
Physical Location & Seq
Forward (+)
19030354 .. 19032275
1922 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00397340.1

Sequence Viewer

Length: 1143 bp
ATGGAGGAAGTAGAGGTAGTCATAGTGGGTGCAGGTCCTGCTGGTCTAGCAACCTCTGCATGTCTTAACCGCCTCAATATCTCAAATGTCATACTCGAAAGAGAAGATTGCTATGCTTCTCTTTGGAAGAATAGGACATATGATCGTTTGAAGCTTCACTTAGCAAAGCAATTTTGTGAACTACCCTATATGCCCTTTCCTAAAAATGCACCCACATATATTCCTAGGAGACAGTTTGTTAAATACCTAGATACTTACGTATCCACCTTCAACATAAACCCTATGTGCCACAGAAGTGTGGAGACTGCTTTTTACAATGAGAATGTAGAAAAATGGTATGTCGTAGTCAACAACACACGATTAGGTGTAAAGGAAACATATTATGGGAAGTTTCTTGTGGTAGCAAGTGGTGAAAATGGTGAAGGCTATGTTCCTAAAATAAAAGGTTTGGATACCTTTAACGGCGAATACATTCATTCGAGCAAGTATGAAAATGGCATGAACTACCGTGGGAAAGATGTTTTGGTTGTCGGATCAGGAAATTCGGGAATGGAAATTGCCTACGACTTATCTAATTACGGCGCAAATACTTCCATTGTTGTACATGTTCTTACCAAAGAAATTGTGTTCTTTGGAATGGTTTTATCGAAATATCTTCCGGTTAAAGTTATCGATGGCATTGTCCTGATCTTAGGAAAATTGAAAATTGGAAATTTATCGAAGTATGGAATTCATAAACCAACAACAGGGCCATTTTATCTCAAGGAACACGAGGGTCAAGCCCCCATCATCGATGTTGGGACCATTGAAAAGATCAAGAGTGACGAAATCAAGGTTTTGCCATCCATAACAAGCATAGAAGGAAATGAAATCCGATTTGAGAATGGCTACCTTAAAAGTTATGATGCTATAATATTTGCTACAGGCTATAAAAGCACTGTGCTAAAGTGGCTCAAGGATGACAATCATCTCTTTAATGACAATGGAATGCCCAATAAAAGTTTTCCGAACCACTGGAAGTCAGAAAATGGTCTTTACTGTGCTGGTTTCTCAAGGCGTGGACTATTTGGAATTTCGCATGATGCACTGAAAATAACAAATGATATCAATTTCTCTCTTGGTCGGAACAAAAAGGGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
FMO-like K_oxygenase Pyr_redox_3

Protein Analysis

380

Amino Acids

42.76

Weight (kDa)

8.78

Isoelectric Point (pI)

28.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 3 - 36 9.9e-06 FAD binding domain
FMO-like PF00743 5 - 311 1.6e-18 Flavin-binding monooxygenase-like
Pyr_redox_2 PF07992 5 - 201 6.4e-13 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_3 PF13738 7 - 202 4.3e-23 Pyridine nucleotide-disulphide oxidoreductase
Lys_Orn_oxgnase PF13434 68 - 201 3.9e-11 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 23
AciI CCGC 1 cut(s) 70
AclWI GGATC 1 cut(s) 541
AcsI RAATTY 4 cut(s) 541, 712, 729, 1071
AfaI GTAC 1 cut(s) 603
AfiI CCNNNNNNNGG 1 cut(s) 746
AflIII ACRYGT 1 cut(s) 604
AgsI TTSAA 4 cut(s) 151, 271, 703, 809
AjuI GAANNNNNNNTTGG 2 cut(s) 506, 538
AleI CACNNNNGTG 1 cut(s) 294
AluBI AGCT 1 cut(s) 154
AluI AGCT 1 cut(s) 154
Alw26I GTCTC 2 cut(s) 223, 296
AlwI GGATC 1 cut(s) 541
AlwNI CAGNNNCTG 1 cut(s) 38
AoxI GGCC 1 cut(s) 749
ApoI RAATTY 4 cut(s) 541, 712, 729, 1071
Asp700I GAANNNNTTC 1 cut(s) 471
AspA2I CCTAGG 1 cut(s) 224
AspLEI GCGC 1 cut(s) 584
AspS9I GGNCC 3 cut(s) 35, 749, 801
AsuHPI GGTGA 2 cut(s) 422, 431
AvaII GGWCC 2 cut(s) 35, 801
AvrII CCTAGG 1 cut(s) 224
BaeI ACNNNNGTAYC 4 cut(s) 243, 243, 276, 276
BauI CACGAG 1 cut(s) 770
BccI CCATC 3 cut(s) 668, 794, 850
BceAI ACGGC 2 cut(s) 478, 595
BciVI GTATCC 2 cut(s) 271, 445
BcoDI GTCTC 2 cut(s) 223, 296
BfaI CTAG 3 cut(s) 47, 225, 248
BfmI CTRYAG 1 cut(s) 921
BfuAI ACCTGC 1 cut(s) 23
BfuI GTATCC 2 cut(s) 271, 445
BlnI CCTAGG 1 cut(s) 224
Bme18I GGWCC 2 cut(s) 35, 801
BmgT120I GGNCC 3 cut(s) 35, 749, 801
BmiI GGNNCC 1 cut(s) 802
BmsI GCATC 2 cut(s) 895, 1072
BpuEI CTTGAG 3 cut(s) 746, 938, 1036
Bsa29I ATCGAT 2 cut(s) 672, 792
BsaAI YACGTR 1 cut(s) 259
BsaBI GATNNNNATC 1 cut(s) 963
BsaJI CCNNGG 2 cut(s) 224, 508
BsaWI WCCGGW 1 cut(s) 658
Bsc4I CCNNNNNNNGG 1 cut(s) 746
Bse1I ACTGG 1 cut(s) 1019
Bse8I GATNNNNATC 1 cut(s) 963
BseCI ATCGAT 2 cut(s) 672, 792
BseDI CCNNGG 2 cut(s) 224, 508
BseGI GGATG 2 cut(s) 842, 964
BseJI GATNNNNATC 1 cut(s) 963
BseLI CCNNNNNNNGG 1 cut(s) 746
BseNI ACTGG 1 cut(s) 1019
BsgI GTGCAG 1 cut(s) 51
BshFI GGCC 1 cut(s) 751
BshVI ATCGAT 2 cut(s) 672, 792
BsiSI CCGG 1 cut(s) 659
BslFI GGGAC 1 cut(s) 814
BslI CCNNNNNNNGG 1 cut(s) 746
BsmAI GTCTC 2 cut(s) 223, 296
BsmFI GGGAC 1 cut(s) 814
BsmI GAATGC 1 cut(s) 993
BsnI GGCC 1 cut(s) 751
Bsp1407I TGTACA 1 cut(s) 601
Bsp143I GATC 4 cut(s) 142, 533, 687, 813
BspACI CCGC 1 cut(s) 70
BspANI GGCC 1 cut(s) 751
BspDI ATCGAT 2 cut(s) 672, 792
BspLI GGNNCC 1 cut(s) 802
BspMI ACCTGC 1 cut(s) 23
BspPI GGATC 1 cut(s) 541
BsrGI TGTACA 1 cut(s) 601
BsrI ACTGG 1 cut(s) 1019
BssECI CCNNGG 2 cut(s) 224, 508
BssMI GATC 4 cut(s) 142, 533, 687, 813
BssSI CACGAG 1 cut(s) 770
BssT1I CCWWGG 1 cut(s) 224
Bst2BI CACGAG 1 cut(s) 770
Bst4CI ACNGT 4 cut(s) 234, 509, 940, 1040
BstAPI GCANNNNNTGC 2 cut(s) 38, 56
BstAUI TGTACA 1 cut(s) 601
BstBAI YACGTR 1 cut(s) 259
BstDEI CTNAG 2 cut(s) 160, 691
BstDSI CCRYGG 1 cut(s) 508
BstF5I GGATG 2 cut(s) 842, 964
BstHHI GCGC 1 cut(s) 584
BstKTI GATC 4 cut(s) 145, 536, 690, 816
BstMAI GTCTC 2 cut(s) 223, 296
BstMBI GATC 4 cut(s) 142, 533, 687, 813
BstMWI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 933, 949
BstNSI RCATGY 2 cut(s) 63, 608
BstSFI CTRYAG 1 cut(s) 921
BstSNI TACGTA 1 cut(s) 259
Bsu15I ATCGAT 2 cut(s) 672, 792
BsuI GTATCC 2 cut(s) 271, 445
BsuRI GGCC 1 cut(s) 751
BsuTUI ATCGAT 2 cut(s) 672, 792
BtgI CCRYGG 1 cut(s) 508
BtsCI GGATG 2 cut(s) 842, 964
BtsIMutI CAGTG 3 cut(s) 936, 1012, 1085
BveI ACCTGC 1 cut(s) 23
CaiI CAGNNNCTG 1 cut(s) 38
CfoI GCGC 1 cut(s) 584
Cfr13I GGNCC 3 cut(s) 35, 749, 801
ClaI ATCGAT 2 cut(s) 672, 792
Csp6I GTAC 1 cut(s) 602
CviAII CATG 4 cut(s) 60, 499, 605, 1079
CviJI RGCY 7 cut(s) 154, 426, 751, 782, 888, 927, 952
CviKI_1 RGCY 7 cut(s) 154, 426, 751, 782, 888, 927, 952
CviQI GTAC 1 cut(s) 602
DdeI CTNAG 2 cut(s) 160, 691
DpnI GATC 4 cut(s) 144, 535, 689, 815
DpnII GATC 4 cut(s) 142, 533, 687, 813
Eco105I TACGTA 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 224
Eco32I GATATC 1 cut(s) 1105
Eco47I GGWCC 2 cut(s) 35, 801
EcoO109I RGGNCCY 1 cut(s) 35
EcoRI GAATTC 1 cut(s) 729
EcoRV GATATC 1 cut(s) 1105
EcoT14I CCWWGG 1 cut(s) 224
ErhI CCWWGG 1 cut(s) 224
FaeI CATG 4 cut(s) 63, 502, 608, 1082
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FaqI GGGAC 1 cut(s) 814
FatI CATG 4 cut(s) 59, 498, 604, 1078
FauNDI CATATG 1 cut(s) 139
FokI GGATG 2 cut(s) 829, 971
FspBI CTAG 3 cut(s) 47, 225, 248
GlaI GCGC 1 cut(s) 583
HaeIII GGCC 1 cut(s) 751
HapII CCGG 1 cut(s) 659
HhaI GCGC 1 cut(s) 584
Hin1II CATG 4 cut(s) 63, 502, 608, 1082
Hin6I GCGC 1 cut(s) 582
HinP1I GCGC 1 cut(s) 582
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 152
HpaII CCGG 1 cut(s) 659
HphI GGTGA 2 cut(s) 422, 431
Hpy166II GTNNAC 3 cut(s) 179, 349, 1061
Hpy188I TCNGA 5 cut(s) 533, 875, 1008, 1024, 1125
Hpy188III TCNNGA 4 cut(s) 537, 546, 685, 817
Hpy8I GTNNAC 3 cut(s) 179, 349, 1061
HpyAV CCTTC 3 cut(s) 277, 416, 854
HpyCH4III ACNGT 4 cut(s) 234, 509, 940, 1040
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 4 cut(s) 32, 59, 209, 1085
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 933, 949
HpyF3I CTNAG 2 cut(s) 160, 691
HpySE526I ACGT 1 cut(s) 258
Hsp92II CATG 4 cut(s) 63, 502, 608, 1082
HspAI GCGC 1 cut(s) 582
Kzo9I GATC 4 cut(s) 142, 533, 687, 813
LweI GCATC 2 cut(s) 895, 1072
MaeI CTAG 3 cut(s) 47, 225, 248
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 1 cut(s) 821
MalI GATC 4 cut(s) 144, 535, 689, 815
MboI GATC 4 cut(s) 142, 533, 687, 813
MboII GAAGA 3 cut(s) 116, 139, 647
MmeI TCCRAC 2 cut(s) 511, 1103
MnlI CCTC 4 cut(s) 7, 64, 83, 766
MroXI GAANNNNTTC 1 cut(s) 471
MseI TTAA 6 cut(s) 66, 240, 459, 663, 894, 975
MslI CAYNNNNRTG 1 cut(s) 294
MspI CCGG 1 cut(s) 659
Mva1269I GAATGC 1 cut(s) 993
MwoI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 933, 949
NdeI CATATG 1 cut(s) 139
NdeII GATC 4 cut(s) 142, 533, 687, 813
NlaIII CATG 4 cut(s) 63, 502, 608, 1082
NlaIV GGNNCC 1 cut(s) 802
NmuCI GTSAC 1 cut(s) 821
NspI RCATGY 2 cut(s) 63, 608
OliI CACNNNNGTG 1 cut(s) 294
PciI ACATGT 1 cut(s) 604
PctI GAATGC 1 cut(s) 993
PdmI GAANNNNTTC 1 cut(s) 471
Ppu21I YACGTR 1 cut(s) 259
PpuMI RGGWCCY 1 cut(s) 35
PscI ACATGT 1 cut(s) 604
Psp5II RGGWCCY 1 cut(s) 35
PspN4I GGNNCC 1 cut(s) 802
PspPI GGNCC 3 cut(s) 35, 749, 801
PspPPI RGGWCCY 1 cut(s) 35
PstNI CAGNNNCTG 1 cut(s) 38
RsaI GTAC 1 cut(s) 603
RsaNI GTAC 1 cut(s) 602
RseI CAYNNNNRTG 1 cut(s) 294
SaqAI TTAA 6 cut(s) 66, 240, 459, 663, 894, 975
Sau3AI GATC 4 cut(s) 142, 533, 687, 813
Sau96I GGNCC 3 cut(s) 35, 749, 801
SfaNI GCATC 2 cut(s) 895, 1072
SfcI CTRYAG 1 cut(s) 921
SinI GGWCC 2 cut(s) 35, 801
SmiMI CAYNNNNRTG 1 cut(s) 294
SmlI CTYRAG 3 cut(s) 761, 953, 1051
SmoI CTYRAG 3 cut(s) 761, 953, 1051
SnaBI TACGTA 1 cut(s) 259
SsiI CCGC 1 cut(s) 70
SspI AATATT 1 cut(s) 915
SspMI CTAG 3 cut(s) 47, 225, 248
StyI CCWWGG 1 cut(s) 224
TaaI ACNGT 4 cut(s) 234, 509, 940, 1040
TaiI ACGT 1 cut(s) 261
TaqI TCGA 6 cut(s) 96, 479, 647, 672, 719, 792
TatI WGTACW 1 cut(s) 601
Tru1I TTAA 6 cut(s) 66, 240, 459, 663, 894, 975
Tru9I TTAA 6 cut(s) 66, 240, 459, 663, 894, 975
TscAI CASTG 3 cut(s) 943, 1019, 1092
TseFI GTSAC 1 cut(s) 821
Tsp45I GTSAC 1 cut(s) 821
TspDTI ATGAA 5 cut(s) 464, 504, 515, 722, 882
TspRI CASTG 3 cut(s) 943, 1019, 1092
VpaK11BI GGWCC 2 cut(s) 35, 801
XapI RAATTY 4 cut(s) 541, 712, 729, 1071
XceI RCATGY 2 cut(s) 63, 608
XmaJI CCTAGG 1 cut(s) 224
XmnI GAANNNNTTC 1 cut(s) 471
XspI CTAG 3 cut(s) 47, 225, 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.