MD10G1010300.v1.1

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005259
Physical Location & Seq
Forward (+)
1431009 .. 1433012
2004 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1010300.v1.1.491

Sequence Viewer

Length: 1152 bp
ATGGAAGAGGTGGATGTGGTCATTGTAGGTGCCGGTCCAGCTGGCATAGCAACGTCTGCATGTCTTAATCACCTCAACATCTCAAATGTTGTAATCGAAAGAGAAGACTCATATGCTTCTCTTTGGAGGAAAAGGTCATATGATCGTTTGAAGCTTCACTTGGCAAAGCAATTTTGTGAACTACCCTATATGCCATTTCCTCCAAATTATCCCAGATATCTCTCCAAGAATGAGTTTGTTCAGTACTTGGAAGCTTATGTATCCCATTTCAAGATTACGCCTCGATACGACCGAGTTGTGGAAACAGCTTCTTATGTAGGTGAAAAAAAATGGTGTGTTAGAGTCCATAACACACTTTCGGACGTACAAGAAATGTACCTTGCTAAATTTCTTGTGGTTGCAAGTGGTGAAAATAGTGAAGGTTACATTCCTCAAGTCGAAGGCTTGAATAGCTTTAAAGGAGAGTTCGTGCACTCCAGCGAGTACGAAAATGGCAAGAAATATGATGGAAAAAATGTTTTGGTCGTCGGTTCTGGGAATTCAGGCATGGAAATTGCTTACGATTTAACAAATTGGGGTGCCAACACTTCGATTGTTGTTCGTAGCCCGGTACATATTCTGACCAAGGAAATTGTATTCCTGGGAATGGTTCTCGCAAAATACCTTCCCCTTAAAGTCCTTGACAACATCGTGGTTATCTTAGGGAAGTTGAAGTTTGGAGATTTATCCAAGTATGGACTTACAAGGCCAAAACTTGGACCATTCTTTCTTAAGGAAAACGAGGGTCAAGCCCCCATCATTGATGTTGGGAGCATTAACAAGATCATAGCTGGAGAAATAAAGGTGGTTCCATCAATAACAAAAATACTAAGAAACCAGATCAAATTTGAGAATGGCTACAGCAACCATTATGATGCTATTGTTTTTGCTACTGGCTATAAAAGTACTGTGCTAAATTGGCTTAAGGATGGTGACCAACTCTTTAATGACAATGGTATGCCGAAGCATAGTTTCCCGAATCACTGGAAGGCGGAGAAGGGACTTTACAGTGCAGGATTCTCAAGGCGCGGATTATTTGGCATTTCAATTGATGCGCGAAATATAGCAGATGACATAAGTTTTGCTTTGAATCAGAACAAGAAAAGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
FMO-like K_oxygenase Pyr_redox_3

Protein Analysis

384

Amino Acids

43.01

Weight (kDa)

8.78

Isoelectric Point (pI)

34.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_2 PF07992 5 - 203 2.3e-14 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 6 - 314 1.7e-25 Flavin-binding monooxygenase-like
Pyr_redox_3 PF13738 7 - 203 1.3e-23 Pyridine nucleotide-disulphide oxidoreductase
Lys_Orn_oxgnase PF13434 73 - 198 1.2e-14 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 29, 578
AccB7I CCANNNNNTGG 1 cut(s) 755
AccII CGCG 2 cut(s) 1068, 1096
AciI CCGC 2 cut(s) 1031, 1068
AcsI RAATTY 3 cut(s) 386, 538, 884
AfaI GTAC 6 cut(s) 245, 366, 377, 485, 612, 946
AfiI CCNNNNNNNGG 3 cut(s) 298, 646, 755
AflII CTTAAG 2 cut(s) 770, 962
AgsI TTSAA 6 cut(s) 151, 271, 448, 712, 1086, 1129
AjnI CCWGG 1 cut(s) 639
AjuI GAANNNNNNNTTGG 2 cut(s) 143, 175
AluBI AGCT 6 cut(s) 41, 154, 254, 308, 453, 830
AluI AGCT 6 cut(s) 41, 154, 254, 308, 453, 830
Alw21I GWGCWC 1 cut(s) 474
Alw44I GTGCAC 1 cut(s) 470
AoxI GGCC 1 cut(s) 746
ApaLI GTGCAC 1 cut(s) 470
ApoI RAATTY 3 cut(s) 386, 538, 884
ArsI GACNNNNNNTTYG 2 cut(s) 1103, 1135
AspLEI GCGC 2 cut(s) 1068, 1096
AspS9I GGNCC 2 cut(s) 35, 758
AsuC2I CCSGG 1 cut(s) 608
AsuHPI GGTGA 4 cut(s) 62, 332, 419, 983
AvaII GGWCC 2 cut(s) 35, 758
BaeGI GKGCMC 1 cut(s) 474
BanI GGYRCC 2 cut(s) 29, 578
BarI GAAGNNNNNNTAC 2 cut(s) 1028, 1060
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 474
BccI CCATC 4 cut(s) 500, 803, 859, 962
BciT130I CCWGG 1 cut(s) 641
BciVI GTATCC 1 cut(s) 271
BcnI CCSGG 1 cut(s) 608
BfmI CTRYAG 1 cut(s) 898
BfrI CTTAAG 2 cut(s) 770, 962
BfuI GTATCC 1 cut(s) 271
BmcAI AGTACT 2 cut(s) 245, 946
Bme1390I CCNGG 2 cut(s) 608, 641
Bme18I GGWCC 2 cut(s) 35, 758
BmgT120I GGNCC 2 cut(s) 35, 758
BmiI GGNNCC 3 cut(s) 31, 580, 849
BmrFI CCNGG 2 cut(s) 608, 641
BmsI GCATC 2 cut(s) 904, 1081
BpiI GAAGAC 1 cut(s) 111
BpmI CTGGAG 2 cut(s) 460, 852
BpuEI CTTGAG 2 cut(s) 417, 1045
BpuMI CCSGG 1 cut(s) 608
BsaJI CCNNGG 2 cut(s) 624, 640
BsaXI ACNNNNNCTCC 2 cut(s) 453, 483
Bsc4I CCNNNNNNNGG 3 cut(s) 298, 646, 755
Bse118I RCCGGY 1 cut(s) 32
Bse1I ACTGG 2 cut(s) 937, 1028
BseBI CCWGG 1 cut(s) 641
BseDI CCNNGG 2 cut(s) 624, 640
BseGI GGATG 2 cut(s) 19, 973
BseLI CCNNNNNNNGG 3 cut(s) 298, 646, 755
BseNI ACTGG 2 cut(s) 937, 1028
BseSI GKGCMC 1 cut(s) 474
BsgI GTGCAG 1 cut(s) 1071
Bsh1236I CGCG 2 cut(s) 1068, 1096
Bsh1285I CGRYCG 1 cut(s) 292
BshFI GGCC 1 cut(s) 748
BshNI GGYRCC 2 cut(s) 29, 578
BsiEI CGRYCG 1 cut(s) 292
BsiHKAI GWGCWC 1 cut(s) 474
BsiSI CCGG 2 cut(s) 33, 608
BslFI GGGAC 1 cut(s) 1053
BslI CCNNNNNNNGG 3 cut(s) 298, 646, 755
BsmFI GGGAC 1 cut(s) 1053
BsnI GGCC 1 cut(s) 748
Bsp1286I GDGCHC 1 cut(s) 474
Bsp143I GATC 3 cut(s) 142, 822, 879
BspACI CCGC 2 cut(s) 1031, 1068
BspANI GGCC 1 cut(s) 748
BspFNI CGCG 2 cut(s) 1068, 1096
BspLI GGNNCC 3 cut(s) 31, 580, 849
BspT107I GGYRCC 2 cut(s) 29, 578
BspTI CTTAAG 2 cut(s) 770, 962
BsrFI RCCGGY 1 cut(s) 32
BsrI ACTGG 2 cut(s) 937, 1028
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 2 cut(s) 624, 640
BssMI GATC 3 cut(s) 142, 822, 879
BssT1I CCWWGG 1 cut(s) 624
Bst2UI CCWGG 1 cut(s) 641
Bst4CI ACNGT 2 cut(s) 949, 1049
BstAFI CTTAAG 2 cut(s) 770, 962
BstAPI GCANNNNNTGC 1 cut(s) 56
BstC8I GCNNGC 1 cut(s) 43
BstDEI CTNAG 2 cut(s) 700, 869
BstEII GGTNACC 1 cut(s) 971
BstF5I GGATG 2 cut(s) 19, 973
BstFNI CGCG 2 cut(s) 1068, 1096
BstHHI GCGC 2 cut(s) 1068, 1096
BstKTI GATC 3 cut(s) 145, 825, 882
BstMBI GATC 3 cut(s) 142, 822, 879
BstMCI CGRYCG 1 cut(s) 292
BstMWI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 450, 958
BstNI CCWGG 1 cut(s) 641
BstNSI RCATGY 1 cut(s) 63
BstPI GGTNACC 1 cut(s) 971
BstSCI CCNGG 2 cut(s) 606, 639
BstSFI CTRYAG 1 cut(s) 898
BstSLI GKGCMC 1 cut(s) 474
BstUI CGCG 2 cut(s) 1068, 1096
BstV2I GAAGAC 1 cut(s) 111
BsuI GTATCC 1 cut(s) 271
BsuRI GGCC 1 cut(s) 748
BtsCI GGATG 2 cut(s) 19, 973
BtsIMutI CAGTG 2 cut(s) 1021, 1054
Cac8I GCNNGC 1 cut(s) 43
CfoI GCGC 2 cut(s) 1068, 1096
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 2 cut(s) 35, 758
Csp6I GTAC 6 cut(s) 244, 365, 376, 484, 611, 945
CviAII CATG 2 cut(s) 60, 547
CviQI GTAC 6 cut(s) 244, 365, 376, 484, 611, 945
DdeI CTNAG 2 cut(s) 700, 869
DpnI GATC 3 cut(s) 144, 824, 881
DpnII GATC 3 cut(s) 142, 822, 879
DraI TTTAAA 1 cut(s) 457
EciI GGCGGA 1 cut(s) 1046
Eco130I CCWWGG 1 cut(s) 624
Eco32I GATATC 1 cut(s) 218
Eco47I GGWCC 2 cut(s) 35, 758
Eco91I GGTNACC 1 cut(s) 971
EcoO65I GGTNACC 1 cut(s) 971
EcoRI GAATTC 1 cut(s) 538
EcoRII CCWGG 1 cut(s) 639
EcoRV GATATC 1 cut(s) 218
EcoT14I CCWWGG 1 cut(s) 624
ErhI CCWWGG 1 cut(s) 624
FaeI CATG 2 cut(s) 63, 550
FalI AAGNNNNNCTT 4 cut(s) 143, 175, 1108, 1140
FaqI GGGAC 1 cut(s) 1053
FatI CATG 2 cut(s) 59, 546
FauNDI CATATG 2 cut(s) 112, 139
FokI GGATG 2 cut(s) 26, 980
GlaI GCGC 2 cut(s) 1067, 1095
GsuI CTGGAG 2 cut(s) 460, 852
HaeIII GGCC 1 cut(s) 748
HapII CCGG 2 cut(s) 33, 608
HhaI GCGC 2 cut(s) 1068, 1096
Hin1II CATG 2 cut(s) 63, 550
Hin6I GCGC 2 cut(s) 1066, 1094
HinP1I GCGC 2 cut(s) 1066, 1094
HindIII AAGCTT 2 cut(s) 152, 252
HinfI GANTC 5 cut(s) 107, 342, 1018, 1056, 1129
HpaII CCGG 2 cut(s) 33, 608
HphI GGTGA 4 cut(s) 62, 332, 419, 983
Hpy166II GTNNAC 2 cut(s) 179, 472
Hpy188I TCNGA 3 cut(s) 361, 621, 1134
Hpy188III TCNNGA 2 cut(s) 271, 1015
Hpy8I GTNNAC 2 cut(s) 179, 472
Hpy99I CGWCG 1 cut(s) 530
HpyAV CCTTC 5 cut(s) 413, 434, 674, 1021, 1030
HpyCH4III ACNGT 2 cut(s) 949, 1049
HpyCH4IV ACGT 2 cut(s) 53, 363
HpyCH4V TGCA 4 cut(s) 59, 401, 472, 1052
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 450, 958
HpyF3I CTNAG 2 cut(s) 700, 869
HpySE526I ACGT 2 cut(s) 53, 363
Hsp92II CATG 2 cut(s) 63, 550
HspAI GCGC 2 cut(s) 1066, 1094
Kzo9I GATC 3 cut(s) 142, 822, 879
LmnI GCTCC 1 cut(s) 810
LweI GCATC 2 cut(s) 904, 1081
MaeII ACGT 2 cut(s) 53, 363
MaeIII GTNAC 2 cut(s) 422, 971
MalI GATC 3 cut(s) 144, 824, 881
MboI GATC 3 cut(s) 142, 822, 879
MboII GAAGA 2 cut(s) 17, 116
MfeI CAATTG 1 cut(s) 1086
MhlI GDGCHC 1 cut(s) 474
MlyI GAGTC 2 cut(s) 101, 351
MnlI CCTC 6 cut(s) 83, 120, 210, 291, 441, 775
MseI TTAA 8 cut(s) 66, 456, 566, 672, 771, 816, 963, 984
MslI CAYNNNNRTG 1 cut(s) 912
MspA1I CMGCKG 1 cut(s) 41
MspCI CTTAAG 2 cut(s) 770, 962
MspI CCGG 2 cut(s) 33, 608
MspR9I CCNGG 2 cut(s) 608, 641
MunI CAATTG 1 cut(s) 1086
MvaI CCWGG 1 cut(s) 641
MvnI CGCG 2 cut(s) 1068, 1096
MwoI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 450, 958
NciI CCSGG 1 cut(s) 608
NdeI CATATG 2 cut(s) 112, 139
NdeII GATC 3 cut(s) 142, 822, 879
NlaIII CATG 2 cut(s) 63, 550
NlaIV GGNNCC 3 cut(s) 31, 580, 849
NmuCI GTSAC 1 cut(s) 971
NspI RCATGY 1 cut(s) 63
PcsI WCGNNNNNNNCGW 1 cut(s) 289
PfeI GAWTC 3 cut(s) 1018, 1056, 1129
PflMI CCANNNNNTGG 1 cut(s) 755
PleI GAGTC 2 cut(s) 101, 350
PpsI GAGTC 2 cut(s) 101, 350
Psp6I CCWGG 1 cut(s) 639
PspEI GGTNACC 1 cut(s) 971
PspGI CCWGG 1 cut(s) 639
PspN4I GGNNCC 3 cut(s) 31, 580, 849
PspPI GGNCC 2 cut(s) 35, 758
PvuII CAGCTG 1 cut(s) 41
RsaI GTAC 6 cut(s) 245, 366, 377, 485, 612, 946
RsaNI GTAC 6 cut(s) 244, 365, 376, 484, 611, 945
RseI CAYNNNNRTG 1 cut(s) 912
SaqAI TTAA 8 cut(s) 66, 456, 566, 672, 771, 816, 963, 984
Sau3AI GATC 3 cut(s) 142, 822, 879
Sau96I GGNCC 2 cut(s) 35, 758
ScaI AGTACT 2 cut(s) 245, 946
SchI GAGTC 2 cut(s) 101, 351
ScrFI CCNGG 2 cut(s) 608, 641
SduI GDGCHC 1 cut(s) 474
SfaNI GCATC 2 cut(s) 904, 1081
SfcI CTRYAG 1 cut(s) 898
SinI GGWCC 2 cut(s) 35, 758
SmiMI CAYNNNNRTG 1 cut(s) 912
SmlI CTYRAG 4 cut(s) 432, 770, 962, 1060
SmoI CTYRAG 4 cut(s) 432, 770, 962, 1060
SsiI CCGC 2 cut(s) 1031, 1068
StyD4I CCNGG 2 cut(s) 606, 639
StyI CCWWGG 1 cut(s) 624
TaaI ACNGT 2 cut(s) 949, 1049
TaiI ACGT 2 cut(s) 56, 366
TaqI TCGA 4 cut(s) 96, 283, 438, 590
TaqII GACCGA 1 cut(s) 306
TatI WGTACW 2 cut(s) 243, 944
TfiI GAWTC 3 cut(s) 1018, 1056, 1129
Tru1I TTAA 8 cut(s) 66, 456, 566, 672, 771, 816, 963, 984
Tru9I TTAA 8 cut(s) 66, 456, 566, 672, 771, 816, 963, 984
TscAI CASTG 2 cut(s) 1028, 1054
TseFI GTSAC 1 cut(s) 971
Tsp45I GTSAC 1 cut(s) 971
TspRI CASTG 2 cut(s) 1028, 1054
Van91I CCANNNNNTGG 1 cut(s) 755
Vha464I CTTAAG 2 cut(s) 770, 962
VneI GTGCAC 1 cut(s) 470
VpaK11BI GGWCC 2 cut(s) 35, 758
XapI RAATTY 3 cut(s) 386, 538, 884
XceI RCATGY 1 cut(s) 63
ZrmI AGTACT 2 cut(s) 245, 946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.