Rw4G031740

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005259
Physical Location & Seq
Reverse (-)
58938651 .. 58940384
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G031740.1

Sequence Viewer

Length: 1155 bp
ATGGAGGAAGTAGAGGTAGTCATAGTAGGTGCAGGTCCTGCTGGTCTAGCAACCTCTGCATGTCTTAACCGCCTCAATATCTCAAATGTCGTACTCGAAAGAGAAGATTGCTATGCTTCTCTTTGGAAGAATAGGACATACGATCGTTTGAAGCTTCACTTAGCAAAGCAATTCTGTGAACTACCGTATTTGCCTTTCCCTAAAAATGCACCCACATATGTTCCTAGGAAAGAGTTTATTCAATACTTGGATACTTATGTGTCCACTTTCAAGATAAACCCTCTGTACCACAGAAGTGTGAAGGCTGCTTTTTATGATGAGGATGTGGAAAAATGGAGTGTCCTTGTCAACAACACAAAACAAGGTGTACAAGAAAGTTACTACGGGAAATCTCTTGTGGTTGCGACCGGAGAAAATAGTGAAGGCTATTTTCCTCAAACAAAAGGTTTGGGTAGCTTTAAAGGTGAAATCATTCATTCAAGTGAGTATGGAAATGGCGAGAAGTACCGTGGGAAAAATGTTTTAGTTGTTGGAGGTGGAAATTCTGGAATGGAAATTGCCTACGACTTATTAAATTTCGGCGCAAATACTTCTATTGTTGTCCGTACCCCGATACATGTTGTTACCAAGGAGATCGTGTTCATTGGAATGGTTCTGTTAAAGTACCTTCCTGTTAAGGTTGTAGATGCCATTGTCATGTTCTTAACCAAGTGGAAATTTGGAAATTTATCCAAGTATGGGATTCAAATTCCAAAAATGGGGCCATTTTATCTCAAGGAAGCCAAAGGTCGATCCCCCATCATCGATGTTGGGACCATCGAAAAGATCAAGAGTGGAGAGATACAGGTTTTGCCATCCATAACAAGCATAGAAGGAAATGAAATCCGGTTTGAGAATGGCTACCTGAAATGTTATGATGGTATGGTATTTGCTACAGGCTATAAAAGCACTGTGCTAAAGTGGCTTAAGGACAAAAACAACCTCTTTAATGATAATGGAATGCCGAAGAAAAGTTTCCCTAACCATTGGAAGTCACAAAATGGCCTTTACTGTGTTGGATTCTCAAGACGTGGATTATTTGGCATTTCACAGGATGCACAAAATATAACAAATGATATAAATTCCTCGCTCAATGAGAGCAAAAGAAGGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
FMO-like K_oxygenase Pyr_redox_3

Protein Analysis

384

Amino Acids

43.18

Weight (kDa)

9.17

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 3 - 36 9.3e-06 FAD binding domain
FMO-like PF00743 5 - 343 1.1e-21 Flavin-binding monooxygenase-like
Pyr_redox_2 PF07992 5 - 206 1.6e-15 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_3 PF13738 7 - 204 1e-22 Pyridine nucleotide-disulphide oxidoreductase
Lys_Orn_oxgnase PF13434 69 - 200 2.5e-14 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 23
AciI CCGC 1 cut(s) 70
AclWI GGATC 1 cut(s) 786
AcsI RAATTY 6 cut(s) 541, 574, 716, 724, 747, 1120
AfaI GTAC 6 cut(s) 93, 287, 369, 506, 607, 665
AfiI CCNNNNNNNGG 2 cut(s) 738, 758
AflII CTTAAG 1 cut(s) 965
AflIII ACRYGT 1 cut(s) 616
AgsI TTSAA 5 cut(s) 151, 242, 271, 480, 746
AjiI CACGTC 1 cut(s) 1070
AleI CACNNNNGTG 1 cut(s) 294
AloI GAACNNNNNNTCC 2 cut(s) 623, 655
AluBI AGCT 2 cut(s) 154, 456
AluI AGCT 2 cut(s) 154, 456
AlwI GGATC 1 cut(s) 786
AlwNI CAGNNNCTG 1 cut(s) 38
AoxI GGCC 2 cut(s) 761, 1042
ApeKI GCWGC 1 cut(s) 305
ApoI RAATTY 6 cut(s) 541, 574, 716, 724, 747, 1120
Asp700I GAANNNNTTC 2 cut(s) 471, 1013
AspA2I CCTAGG 1 cut(s) 224
AspLEI GCGC 1 cut(s) 584
AspS9I GGNCC 3 cut(s) 35, 761, 813
AsuHPI GGTGA 1 cut(s) 476
AvaII GGWCC 2 cut(s) 35, 813
AvrII CCTAGG 1 cut(s) 224
BaeI ACNNNNGTAYC 2 cut(s) 243, 276
BbvI GCAGC 1 cut(s) 292
BccI CCATC 4 cut(s) 806, 824, 862, 911
BciVI GTATCC 1 cut(s) 244
BfaI CTAG 2 cut(s) 47, 225
BfmI CTRYAG 1 cut(s) 933
BfrI CTTAAG 1 cut(s) 965
BfuAI ACCTGC 1 cut(s) 23
BfuI GTATCC 1 cut(s) 244
BisI GCNGC 1 cut(s) 306
BlnI CCTAGG 1 cut(s) 224
BlsI GCNGC 1 cut(s) 307
Bme18I GGWCC 2 cut(s) 35, 813
BmgBI CACGTC 1 cut(s) 1070
BmgT120I GGNCC 3 cut(s) 35, 761, 813
BmiI GGNNCC 2 cut(s) 762, 814
BmsI GCATC 2 cut(s) 676, 1084
BpuEI CTTGAG 2 cut(s) 758, 1048
Bsa29I ATCGAT 1 cut(s) 804
BsaJI CCNNGG 3 cut(s) 224, 508, 627
BsaWI WCCGGW 2 cut(s) 407, 885
BsaXI ACNNNNNCTCC 2 cut(s) 623, 653
Bsc4I CCNNNNNNNGG 2 cut(s) 738, 758
BseCI ATCGAT 1 cut(s) 804
BseDI CCNNGG 3 cut(s) 224, 508, 627
BseGI GGATG 3 cut(s) 328, 854, 1099
BseLI CCNNNNNNNGG 2 cut(s) 738, 758
BseXI GCAGC 1 cut(s) 292
BsgI GTGCAG 1 cut(s) 51
Bsh1285I CGRYCG 2 cut(s) 145, 408
BshFI GGCC 2 cut(s) 763, 1044
BshVI ATCGAT 1 cut(s) 804
BsiEI CGRYCG 2 cut(s) 145, 408
BsiSI CCGG 2 cut(s) 408, 886
BslFI GGGAC 1 cut(s) 826
BslI CCNNNNNNNGG 2 cut(s) 738, 758
BsmFI GGGAC 1 cut(s) 826
BsmI GAATGC 1 cut(s) 1005
BsnI GGCC 2 cut(s) 763, 1044
Bsp1407I TGTACA 1 cut(s) 367
Bsp143I GATC 4 cut(s) 142, 633, 791, 825
BspACI CCGC 1 cut(s) 70
BspANI GGCC 2 cut(s) 763, 1044
BspDI ATCGAT 1 cut(s) 804
BspLI GGNNCC 2 cut(s) 762, 814
BspMI ACCTGC 1 cut(s) 23
BspPI GGATC 1 cut(s) 786
BspTI CTTAAG 1 cut(s) 965
BsrGI TGTACA 1 cut(s) 367
BssECI CCNNGG 3 cut(s) 224, 508, 627
BssMI GATC 4 cut(s) 142, 633, 791, 825
BssT1I CCWWGG 2 cut(s) 224, 627
Bst4CI ACNGT 4 cut(s) 186, 509, 952, 1052
BstAFI CTTAAG 1 cut(s) 965
BstAPI GCANNNNNTGC 2 cut(s) 38, 56
BstAUI TGTACA 1 cut(s) 367
BstDEI CTNAG 1 cut(s) 160
BstDSI CCRYGG 1 cut(s) 508
BstF5I GGATG 3 cut(s) 328, 854, 1099
BstHHI GCGC 1 cut(s) 584
BstKTI GATC 4 cut(s) 145, 636, 794, 828
BstMBI GATC 4 cut(s) 142, 633, 791, 825
BstMCI CGRYCG 2 cut(s) 145, 408
BstMWI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 945, 961
BstNSI RCATGY 2 cut(s) 63, 620
BstSFI CTRYAG 1 cut(s) 933
BstV1I GCAGC 1 cut(s) 292
Bsu15I ATCGAT 1 cut(s) 804
BsuI GTATCC 1 cut(s) 244
BsuRI GGCC 2 cut(s) 763, 1044
BsuTUI ATCGAT 1 cut(s) 804
BtgI CCRYGG 1 cut(s) 508
BtrI CACGTC 1 cut(s) 1070
BtsCI GGATG 3 cut(s) 328, 854, 1099
BtsIMutI CAGTG 1 cut(s) 948
BveI ACCTGC 1 cut(s) 23
CaiI CAGNNNCTG 1 cut(s) 38
CfoI GCGC 1 cut(s) 584
Cfr13I GGNCC 3 cut(s) 35, 761, 813
ClaI ATCGAT 1 cut(s) 804
Csp6I GTAC 6 cut(s) 92, 286, 368, 505, 606, 664
CviAII CATG 3 cut(s) 60, 617, 697
CviQI GTAC 6 cut(s) 92, 286, 368, 505, 606, 664
DdeI CTNAG 1 cut(s) 160
DpnI GATC 4 cut(s) 144, 635, 793, 827
DpnII GATC 4 cut(s) 142, 633, 791, 825
DraI TTTAAA 1 cut(s) 460
Eco130I CCWWGG 2 cut(s) 224, 627
Eco47I GGWCC 2 cut(s) 35, 813
EcoO109I RGGNCCY 1 cut(s) 35
EcoT14I CCWWGG 2 cut(s) 224, 627
ErhI CCWWGG 2 cut(s) 224, 627
FaeI CATG 3 cut(s) 63, 620, 700
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FaqI GGGAC 1 cut(s) 826
FatI CATG 3 cut(s) 59, 616, 696
FauNDI CATATG 1 cut(s) 217
Fnu4HI GCNGC 1 cut(s) 306
FokI GGATG 3 cut(s) 335, 841, 1106
Fsp4HI GCNGC 1 cut(s) 306
FspBI CTAG 2 cut(s) 47, 225
GlaI GCGC 1 cut(s) 583
GluI GCNGC 1 cut(s) 306
HaeIII GGCC 2 cut(s) 763, 1044
HapII CCGG 2 cut(s) 408, 886
HhaI GCGC 1 cut(s) 584
Hin1II CATG 3 cut(s) 63, 620, 700
Hin6I GCGC 1 cut(s) 582
HinP1I GCGC 1 cut(s) 582
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 152
HinfI GANTC 2 cut(s) 742, 1059
HpaII CCGG 2 cut(s) 408, 886
HphI GGTGA 1 cut(s) 476
Hpy166II GTNNAC 4 cut(s) 179, 264, 349, 368
Hpy188III TCNNGA 4 cut(s) 271, 546, 829, 1065
Hpy8I GTNNAC 4 cut(s) 179, 264, 349, 368
HpyAV CCTTC 5 cut(s) 295, 416, 677, 866, 1140
HpyCH4III ACNGT 4 cut(s) 186, 509, 952, 1052
HpyCH4IV ACGT 1 cut(s) 1069
HpyCH4V TGCA 4 cut(s) 32, 59, 209, 1097
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 945, 961
HpyF3I CTNAG 1 cut(s) 160
HpySE526I ACGT 1 cut(s) 1069
Hsp92II CATG 3 cut(s) 63, 620, 700
HspAI GCGC 1 cut(s) 582
Kzo9I GATC 4 cut(s) 142, 633, 791, 825
Lsp1109I GCAGC 1 cut(s) 292
LweI GCATC 2 cut(s) 676, 1084
MaeI CTAG 2 cut(s) 47, 225
MaeII ACGT 1 cut(s) 1069
MaeIII GTNAC 3 cut(s) 377, 622, 1032
MalI GATC 4 cut(s) 144, 635, 793, 827
MboI GATC 4 cut(s) 142, 633, 791, 825
MboII GAAGA 3 cut(s) 116, 139, 1018
MluCI AATT 8 cut(s) 170, 541, 555, 574, 716, 724, 747, 1120
MmeI TCCRAC 2 cut(s) 511, 1036
MnlI CCTC 9 cut(s) 7, 64, 83, 291, 313, 444, 527, 992, 1135
MroXI GAANNNNTTC 2 cut(s) 471, 1013
MseI TTAA 8 cut(s) 66, 459, 572, 659, 675, 704, 966, 987
MslI CAYNNNNRTG 4 cut(s) 294, 480, 647, 695
MspCI CTTAAG 1 cut(s) 965
MspI CCGG 2 cut(s) 408, 886
Mva1269I GAATGC 1 cut(s) 1005
MwoI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 945, 961
NdeI CATATG 1 cut(s) 217
NdeII GATC 4 cut(s) 142, 633, 791, 825
NlaIII CATG 3 cut(s) 63, 620, 700
NlaIV GGNNCC 2 cut(s) 762, 814
NmuCI GTSAC 1 cut(s) 1032
NspI RCATGY 2 cut(s) 63, 620
OliI CACNNNNGTG 1 cut(s) 294
PciI ACATGT 1 cut(s) 616
PctI GAATGC 1 cut(s) 1005
PdmI GAANNNNTTC 2 cut(s) 471, 1013
PfeI GAWTC 2 cut(s) 742, 1059
PkrI GCNGC 1 cut(s) 307
Ple19I CGATCG 1 cut(s) 145
PpuMI RGGWCCY 1 cut(s) 35
PscI ACATGT 1 cut(s) 616
Psp5II RGGWCCY 1 cut(s) 35
PspN4I GGNNCC 2 cut(s) 762, 814
PspPI GGNCC 3 cut(s) 35, 761, 813
PspPPI RGGWCCY 1 cut(s) 35
PstNI CAGNNNCTG 1 cut(s) 38
PvuI CGATCG 1 cut(s) 145
RsaI GTAC 6 cut(s) 93, 287, 369, 506, 607, 665
RsaNI GTAC 6 cut(s) 92, 286, 368, 505, 606, 664
RseI CAYNNNNRTG 4 cut(s) 294, 480, 647, 695
SaqAI TTAA 8 cut(s) 66, 459, 572, 659, 675, 704, 966, 987
SatI GCNGC 1 cut(s) 306
Sau3AI GATC 4 cut(s) 142, 633, 791, 825
Sau96I GGNCC 3 cut(s) 35, 761, 813
SfaNI GCATC 2 cut(s) 676, 1084
SfcI CTRYAG 1 cut(s) 933
SinI GGWCC 2 cut(s) 35, 813
SmiMI CAYNNNNRTG 4 cut(s) 294, 480, 647, 695
SmlI CTYRAG 3 cut(s) 773, 965, 1063
SmoI CTYRAG 3 cut(s) 773, 965, 1063
Sse9I AATT 8 cut(s) 170, 541, 555, 574, 716, 724, 747, 1120
SsiI CCGC 1 cut(s) 70
SspMI CTAG 2 cut(s) 47, 225
StyI CCWWGG 2 cut(s) 224, 627
TaaI ACNGT 4 cut(s) 186, 509, 952, 1052
TaiI ACGT 1 cut(s) 1072
TaqI TCGA 4 cut(s) 96, 790, 804, 819
TasI AATT 8 cut(s) 170, 541, 555, 574, 716, 724, 747, 1120
TatI WGTACW 1 cut(s) 367
TfiI GAWTC 2 cut(s) 742, 1059
Tru1I TTAA 8 cut(s) 66, 459, 572, 659, 675, 704, 966, 987
Tru9I TTAA 8 cut(s) 66, 459, 572, 659, 675, 704, 966, 987
TscAI CASTG 1 cut(s) 955
TseFI GTSAC 1 cut(s) 1032
TseI GCWGC 1 cut(s) 305
Tsp45I GTSAC 1 cut(s) 1032
TspDTI ATGAA 3 cut(s) 464, 631, 894
TspGWI ACGGA 1 cut(s) 593
TspRI CASTG 1 cut(s) 955
Vha464I CTTAAG 1 cut(s) 965
VpaK11BI GGWCC 2 cut(s) 35, 813
XapI RAATTY 6 cut(s) 541, 574, 716, 724, 747, 1120
XceI RCATGY 2 cut(s) 63, 620
XmaJI CCTAGG 1 cut(s) 224
XmnI GAANNNNTTC 2 cut(s) 471, 1013
XspI CTAG 2 cut(s) 47, 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.