MD15G1274600.v1.1

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005259
Physical Location & Seq
Reverse (-)
24088780 .. 24090298
1519 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1274600.v1.1.491

Sequence Viewer

Length: 1158 bp
ATGGAGGAAGTGCAGGTGGTCATCGTCGGTGCTGGTCCGGCTGGCTTAGCAACCTCAGCATGTCTTAATCATCTTAACATTACAAATGCGGTACTCGAAAGAGAAGATTGCTATGCTTCTCTTTGGAAGAAAAAGTCATATGATCGTTTGAAGCTACACTTAGCAAAGCAATTTTGCGAACTTCCCTATATGCCTTTCCCTTCGGATACACCTACATACGTCCCCAAGAATTTGTTTGTTCAATACTTGGATACTTATGTATCCCATTTCAACATAAACCCTAAGTGCAACCGAAGCGTGAAGTCTGCTTTCTACGATGTGGATTTAGAGAAATGGTATGTCAAGGTCGAGAACACGCTTTCGGGTGCACAAGAAATGTATCTTGGGAAGTTTCTTGTGGTGGCAAGTGGTGAAAATAGCGTAGGTTATATTCCTCAAGTGCGTGGCTTGGATGGGTTCAATGGGCAGGCCATTCATTCAAGCAAGTACGAAAACGGCAAGAAATATGGTGGGAAAAATGTTTTGGTCGTTGGTTCTGGTAATTCAGGCATGGAAATTGCTTATGATTTGTCGAATTCAGGAGCTAATACTTCCATTGTTGTCCGTAGCCCGGTACATGTTCTCAGCAAGGAAATTGTGTTCTTTGGAATGGTTTTGTTAAAACTCCTCCCAGTTACAATAGTCGACAAGGTTGTCGTAGGATTAGGCAAATTGAAGTATGGAAATTTATCCAAGTACGGGATTCAAAAGCCAAAACAGGGACCTTTTTATCTCAAGGCTACTAAGGGTCGATCCCCCACTATCGACGTTGGTTGCATTAAGAAGATTAAAACCAGGGAAATAACGGTCTTGCCTTCCATAACAAGCATAGAAGGAAATCTAATCAGATTTGAAAATGGCATCTATAATTGGTACGATGCTATCATATTTGCTACAGGCTACAAAAGCAGCGTTCTAAATTGGCTTAAGGATGACAACTGCTACTTTAACGACAATGGAATGCCACGAAAGAGGTTCCCAGATCACTGGAAATCAGAAAATGGTCTTTACAGCGCTGGGTTCTCAAGCCGTGGATTGCTTGGCATTGCACATGATGCACATTGTATAGCAAATGACATTAGTTCGTCTTTGAACTTACACTACAAGAAAATGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
FMO-like K_oxygenase Pyr_redox_3

Protein Analysis

386

Amino Acids

42.85

Weight (kDa)

9.09

Isoelectric Point (pI)

30.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMO-like PF00743 5 - 317 1.5e-21 Flavin-binding monooxygenase-like
Pyr_redox_2 PF07992 5 - 204 1e-13 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_3 PF13738 7 - 204 2.6e-21 Pyridine nucleotide-disulphide oxidoreductase
Lys_Orn_oxgnase PF13434 72 - 200 6.7e-11 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 4
AasI GACNNNNNNGTC 1 cut(s) 692
Acc36I ACCTGC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 684
AciI CCGC 1 cut(s) 89
AclWI GGATC 1 cut(s) 786
AcsI RAATTY 3 cut(s) 229, 574, 724
AfaI GTAC 5 cut(s) 93, 488, 615, 737, 914
AfeI AGCGCT 1 cut(s) 1054
AfiI CCNNNNNNNGG 3 cut(s) 610, 738, 758
AflII CTTAAG 1 cut(s) 965
AflIII ACRYGT 1 cut(s) 616
AgsI TTSAA 9 cut(s) 151, 242, 271, 460, 480, 715, 746, 893, 1132
AjnI CCWGG 1 cut(s) 833
AjuI GAANNNNNNNTTGG 4 cut(s) 366, 398, 506, 538
AloI GAACNNNNNNTCC 2 cut(s) 623, 655
AluBI AGCT 2 cut(s) 154, 584
AluI AGCT 2 cut(s) 154, 584
Alw21I GWGCWC 1 cut(s) 370
Alw44I GTGCAC 1 cut(s) 366
AlwI GGATC 1 cut(s) 786
Aor51HI AGCGCT 1 cut(s) 1054
AoxI GGCC 1 cut(s) 468
ApaLI GTGCAC 1 cut(s) 366
ApeKI GCWGC 1 cut(s) 948
ApoI RAATTY 3 cut(s) 229, 574, 724
AspLEI GCGC 1 cut(s) 1055
AspS9I GGNCC 2 cut(s) 35, 761
AsuC2I CCSGG 1 cut(s) 611
AsuHPI GGTGA 1 cut(s) 422
AvaII GGWCC 2 cut(s) 35, 761
BaeGI GKGCMC 1 cut(s) 370
BaeI ACNNNNGTAYC 4 cut(s) 243, 243, 276, 276
Bbv12I GWGCWC 1 cut(s) 370
BbvCI CCTCAGC 1 cut(s) 55
BbvI GCAGC 1 cut(s) 960
BccI CCATC 1 cut(s) 446
BceAI ACGGC 2 cut(s) 511, 1053
BciT130I CCWGG 1 cut(s) 835
BciVI GTATCC 3 cut(s) 199, 244, 271
BcnI CCSGG 1 cut(s) 611
BfmI CTRYAG 1 cut(s) 933
BfoI RGCGCY 1 cut(s) 1056
BfrI CTTAAG 1 cut(s) 965
BfuAI ACCTGC 1 cut(s) 4
BfuI GTATCC 3 cut(s) 199, 244, 271
BisI GCNGC 1 cut(s) 949
BlpI GCTNAGC 1 cut(s) 46
BlsI GCNGC 1 cut(s) 950
Bme1390I CCNGG 2 cut(s) 611, 835
Bme18I GGWCC 2 cut(s) 35, 761
BmgT120I GGNCC 2 cut(s) 35, 761
BmiI GGNNCC 2 cut(s) 762, 1016
BmrFI CCNGG 2 cut(s) 611, 835
BmrI ACTGGG 1 cut(s) 665
BmsI GCATC 3 cut(s) 907, 909, 1084
BmuI ACTGGG 1 cut(s) 665
Bpu10I CCTNAGC 1 cut(s) 55
Bpu1102I GCTNAGC 1 cut(s) 46
BpuEI CTTGAG 3 cut(s) 420, 758, 1048
BpuMI CCSGG 1 cut(s) 611
BsaJI CCNNGG 2 cut(s) 834, 1069
Bsc4I CCNNNNNNNGG 3 cut(s) 610, 738, 758
Bse1I ACTGG 2 cut(s) 671, 1031
Bse3DI GCAATG 1 cut(s) 1083
BseBI CCWGG 1 cut(s) 835
BseDI CCNNGG 2 cut(s) 834, 1069
BseGI GGATG 2 cut(s) 457, 976
BseLI CCNNNNNNNGG 3 cut(s) 610, 738, 758
BseMI GCAATG 1 cut(s) 1083
BseMII CTCAG 2 cut(s) 69, 637
BseNI ACTGG 2 cut(s) 671, 1031
BseRI GAGGAG 1 cut(s) 656
BseSI GKGCMC 1 cut(s) 370
BseXI GCAGC 1 cut(s) 960
BseYI CCCAGC 1 cut(s) 1055
BsgI GTGCAG 1 cut(s) 32
BshFI GGCC 1 cut(s) 470
BsiHKAI GWGCWC 1 cut(s) 370
BsiSI CCGG 2 cut(s) 38, 611
BslFI GGGAC 2 cut(s) 206, 774
BslI CCNNNNNNNGG 3 cut(s) 610, 738, 758
BsmFI GGGAC 2 cut(s) 206, 774
BsmI GAATGC 1 cut(s) 1005
BsnI GGCC 1 cut(s) 470
Bsp1286I GDGCHC 1 cut(s) 370
Bsp143I GATC 3 cut(s) 142, 791, 1021
Bsp1720I GCTNAGC 1 cut(s) 46
BspACI CCGC 1 cut(s) 89
BspANI GGCC 1 cut(s) 470
BspCNI CTCAG 2 cut(s) 68, 636
BspLI GGNNCC 2 cut(s) 762, 1016
BspMI ACCTGC 1 cut(s) 4
BspPI GGATC 1 cut(s) 786
BspTI CTTAAG 1 cut(s) 965
BsrDI GCAATG 1 cut(s) 1083
BsrI ACTGG 2 cut(s) 671, 1031
BssECI CCNNGG 2 cut(s) 834, 1069
BssMI GATC 3 cut(s) 142, 791, 1021
Bst2UI CCWGG 1 cut(s) 835
Bst4CI ACNGT 1 cut(s) 847
BstAFI CTTAAG 1 cut(s) 965
BstAPI GCANNNNNTGC 1 cut(s) 1094
BstC8I GCNNGC 2 cut(s) 43, 468
BstDEI CTNAG 6 cut(s) 46, 55, 160, 282, 623, 783
BstDSI CCRYGG 1 cut(s) 1069
BstF5I GGATG 2 cut(s) 457, 976
BstH2I RGCGCY 1 cut(s) 1056
BstHHI GCGC 1 cut(s) 1055
BstKTI GATC 3 cut(s) 145, 794, 1024
BstMBI GATC 3 cut(s) 142, 791, 1021
BstMWI GCNNNNNNNGC 6 cut(s) 38, 47, 56, 294, 945, 1094
BstNI CCWGG 1 cut(s) 835
BstNSI RCATGY 2 cut(s) 63, 620
BstSCI CCNGG 2 cut(s) 609, 833
BstSFI CTRYAG 1 cut(s) 933
BstSLI GKGCMC 1 cut(s) 370
BstV1I GCAGC 1 cut(s) 960
BstXI CCANNNNNNTGG 1 cut(s) 1026
BsuI GTATCC 3 cut(s) 199, 244, 271
BsuRI GGCC 1 cut(s) 470
BtgI CCRYGG 1 cut(s) 1069
BtsCI GGATG 2 cut(s) 457, 976
BtsIMutI CAGTG 1 cut(s) 1024
BveI ACCTGC 1 cut(s) 4
Cac8I GCNNGC 2 cut(s) 43, 468
CfoI GCGC 1 cut(s) 1055
Cfr13I GGNCC 2 cut(s) 35, 761
Csp6I GTAC 5 cut(s) 92, 487, 614, 736, 913
CviAII CATG 4 cut(s) 60, 550, 617, 1091
CviQI GTAC 5 cut(s) 92, 487, 614, 736, 913
DdeI CTNAG 6 cut(s) 46, 55, 160, 282, 623, 783
DpnI GATC 3 cut(s) 144, 793, 1023
DpnII GATC 3 cut(s) 142, 791, 1021
DrdI GACNNNNNNGTC 1 cut(s) 692
DseDI GACNNNNNNGTC 1 cut(s) 692
Eco47I GGWCC 2 cut(s) 35, 761
Eco47III AGCGCT 1 cut(s) 1054
EcoO109I RGGNCCY 1 cut(s) 761
EcoRI GAATTC 1 cut(s) 574
EcoRII CCWGG 1 cut(s) 833
FaeI CATG 4 cut(s) 63, 553, 620, 1094
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FaqI GGGAC 2 cut(s) 206, 774
FatI CATG 4 cut(s) 59, 549, 616, 1090
FauNDI CATATG 1 cut(s) 139
FblI GTMKAC 1 cut(s) 684
Fnu4HI GCNGC 1 cut(s) 949
FokI GGATG 2 cut(s) 464, 983
Fsp4HI GCNGC 1 cut(s) 949
GlaI GCGC 1 cut(s) 1054
GluI GCNGC 1 cut(s) 949
GsaI CCCAGC 1 cut(s) 1059
HaeII RGCGCY 1 cut(s) 1056
HaeIII GGCC 1 cut(s) 470
HapII CCGG 2 cut(s) 38, 611
HhaI GCGC 1 cut(s) 1055
Hin1II CATG 4 cut(s) 63, 553, 620, 1094
Hin6I GCGC 1 cut(s) 1053
HinP1I GCGC 1 cut(s) 1053
HincII GTYRAC 1 cut(s) 685
HindII GTYRAC 1 cut(s) 685
HinfI GANTC 1 cut(s) 742
HpaII CCGG 2 cut(s) 38, 611
HphI GGTGA 1 cut(s) 422
Hpy166II GTNNAC 2 cut(s) 368, 685
Hpy188I TCNGA 3 cut(s) 205, 887, 1036
Hpy188III TCNNGA 2 cut(s) 349, 579
Hpy8I GTNNAC 2 cut(s) 368, 685
Hpy99I CGWCG 2 cut(s) 29, 809
HpyAV CCTTC 3 cut(s) 210, 864, 866
HpyCH4III ACNGT 1 cut(s) 847
HpyCH4IV ACGT 2 cut(s) 219, 807
HpyCH4V TGCA 6 cut(s) 13, 288, 368, 816, 1088, 1097
HpyF10VI GCNNNNNNNGC 6 cut(s) 38, 47, 56, 294, 945, 1094
HpyF3I CTNAG 6 cut(s) 46, 55, 160, 282, 623, 783
HpySE526I ACGT 2 cut(s) 219, 807
Hsp92II CATG 4 cut(s) 63, 553, 620, 1094
HspAI GCGC 1 cut(s) 1053
Kzo9I GATC 3 cut(s) 142, 791, 1021
LmnI GCTCC 1 cut(s) 581
Lsp1109I GCAGC 1 cut(s) 960
LweI GCATC 3 cut(s) 907, 909, 1084
MaeII ACGT 2 cut(s) 219, 807
MaeIII GTNAC 1 cut(s) 673
MalI GATC 3 cut(s) 144, 793, 1023
MboI GATC 3 cut(s) 142, 791, 1021
MboII GAAGA 3 cut(s) 116, 139, 835
MhlI GDGCHC 1 cut(s) 370
MnlI CCTC 4 cut(s) 64, 444, 677, 1005
MseI TTAA 7 cut(s) 66, 75, 659, 819, 828, 966, 987
MspCI CTTAAG 1 cut(s) 965
MspI CCGG 2 cut(s) 38, 611
MspR9I CCNGG 2 cut(s) 611, 835
Mva1269I GAATGC 1 cut(s) 1005
MvaI CCWGG 1 cut(s) 835
MwoI GCNNNNNNNGC 6 cut(s) 38, 47, 56, 294, 945, 1094
NciI CCSGG 1 cut(s) 611
NdeI CATATG 1 cut(s) 139
NdeII GATC 3 cut(s) 142, 791, 1021
NlaIII CATG 4 cut(s) 63, 553, 620, 1094
NlaIV GGNNCC 2 cut(s) 762, 1016
NspI RCATGY 2 cut(s) 63, 620
PaqCI CACCTGC 1 cut(s) 4
PciI ACATGT 1 cut(s) 616
PctI GAATGC 1 cut(s) 1005
PfeI GAWTC 1 cut(s) 742
PkrI GCNGC 1 cut(s) 950
PpuMI RGGWCCY 1 cut(s) 761
PscI ACATGT 1 cut(s) 616
Psp5II RGGWCCY 1 cut(s) 761
Psp6I CCWGG 1 cut(s) 833
PspFI CCCAGC 1 cut(s) 1055
PspGI CCWGG 1 cut(s) 833
PspN4I GGNNCC 2 cut(s) 762, 1016
PspPI GGNCC 2 cut(s) 35, 761
PspPPI RGGWCCY 1 cut(s) 761
PsrI GAACNNNNNNTAC 2 cut(s) 1124, 1156
RsaI GTAC 5 cut(s) 93, 488, 615, 737, 914
RsaNI GTAC 5 cut(s) 92, 487, 614, 736, 913
SalI GTCGAC 1 cut(s) 683
SaqAI TTAA 7 cut(s) 66, 75, 659, 819, 828, 966, 987
SatI GCNGC 1 cut(s) 949
Sau3AI GATC 3 cut(s) 142, 791, 1021
Sau96I GGNCC 2 cut(s) 35, 761
ScrFI CCNGG 2 cut(s) 611, 835
SduI GDGCHC 1 cut(s) 370
SfaNI GCATC 3 cut(s) 907, 909, 1084
SfcI CTRYAG 1 cut(s) 933
SinI GGWCC 2 cut(s) 35, 761
SmlI CTYRAG 4 cut(s) 435, 773, 965, 1063
SmoI CTYRAG 4 cut(s) 435, 773, 965, 1063
SsiI CCGC 1 cut(s) 89
StyD4I CCNGG 2 cut(s) 609, 833
TaaI ACNGT 1 cut(s) 847
TaiI ACGT 2 cut(s) 222, 810
TaqI TCGA 6 cut(s) 96, 348, 572, 684, 790, 804
TfiI GAWTC 1 cut(s) 742
Tru1I TTAA 7 cut(s) 66, 75, 659, 819, 828, 966, 987
Tru9I TTAA 7 cut(s) 66, 75, 659, 819, 828, 966, 987
TscAI CASTG 1 cut(s) 1031
TseI GCWGC 1 cut(s) 948
TspDTI ATGAA 1 cut(s) 464
TspGWI ACGGA 1 cut(s) 593
TspRI CASTG 1 cut(s) 1031
Vha464I CTTAAG 1 cut(s) 965
VneI GTGCAC 1 cut(s) 366
VpaK11BI GGWCC 2 cut(s) 35, 761
XapI RAATTY 3 cut(s) 229, 574, 724
XceI RCATGY 2 cut(s) 63, 620
XmiI GTMKAC 1 cut(s) 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.