FvH4_2g14914

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006386
Physical Location & Seq
Forward (+)
13116450 .. 13117175
726 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g14914.t1

Sequence Viewer

Length: 726 bp
ATGGCCTCAGCTGCAACAGAGTTCTTGATTGGCAAAATAGGGGAAGTTGTTGAGAAGGAAGCATCCTCCATTGCTGGTGTTCGCGATCAAGTGGATGAGATTAAGCAGGAGCTGATAAGCATGAAATCCTTCCTAGAGGATGCTGAGGGACAGAAAATAGAGAGGGAAGGAGAGAAGGAATGGGTAGCAAGCATTAGAAACTCTGTTTATGATGTTGAAGATATTATTGATGAATTCATGTACCGCATGCATGAGCAGCGAAATGGAGGTCGACTAGCAAGGTTGCTATGCAAAACCACTCTTATTCCAAAGAATTGGTACAGGGGTCGGTTTGCAAGGTGGCTTCGCAAAACCATTCACATTCCAACCAGATTTCACTGCAGGGGTCGGTATGTAAGGTGGCTTCGCAAATGCATACTAATTCCAGAGTATCTTTGGTACAGGCGTCAAATTGCCAAAAAGCTGCAGAAAATCACAAAAATCATCAGAGAGATTCCAGAGAGAAAAAAGAGATATGGTATTAGTCATGTGGAAGGAACAGGTTCGAAAGATATTCGCAATCAAGTGCAGAACCAAGCAGAGTCATCTCTTTTCATTACAGAAGATGAACTTGTAGGATTTGAAGACAAGAAGAAACTGCTCAGAGGTAAAACATCAAATCGTTGTTTCTGTGGTTGGGATGGGAGGGTCAGGAAAGACAACCCTAGTCGCCAAGATCTTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

242

Amino Acids

28.47

Weight (kDa)

9.64

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 7 - 88 3.7e-24 Rx N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 271
AccII CGCG 1 cut(s) 84
AciI CCGC 1 cut(s) 244
AcsI RAATTY 1 cut(s) 233
AcyI GRCGYC 1 cut(s) 445
AfaI GTAC 3 cut(s) 242, 320, 440
AgsI TTSAA 2 cut(s) 218, 623
AluBI AGCT 3 cut(s) 11, 112, 463
AluI AGCT 3 cut(s) 11, 112, 463
AlwNI CAGNNNCTG 1 cut(s) 112
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 11, 256, 463
ApoI RAATTY 1 cut(s) 233
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
Asp700I GAANNNNTTC 2 cut(s) 128, 541
AsuII TTCGAA 1 cut(s) 545
BaeI ACNNNNGTAYC 4 cut(s) 310, 343, 430, 463
BarI GAAGNNNNNNTAC 2 cut(s) 387, 419
BbsI GAAGAC 1 cut(s) 630
BbvCI CCTCAGC 2 cut(s) 7, 144
BbvI GCAGC 2 cut(s) 268, 450
BccI CCATC 1 cut(s) 674
BfaI CTAG 3 cut(s) 134, 275, 705
BfmI CTRYAG 2 cut(s) 379, 464
BglII AGATCT 1 cut(s) 715
BisI GCNGC 3 cut(s) 12, 257, 464
BlsI GCNGC 3 cut(s) 13, 258, 465
BmsI GCATC 2 cut(s) 71, 130
BpiI GAAGAC 1 cut(s) 630
Bpu10I CCTNAGC 2 cut(s) 7, 144
Bpu14I TTCGAA 1 cut(s) 545
BsaHI GRCGYC 1 cut(s) 445
Bse3DI GCAATG 1 cut(s) 69
BseGI GGATG 4 cut(s) 62, 100, 145, 685
BseMI GCAATG 1 cut(s) 69
BseMII CTCAG 3 cut(s) 21, 135, 655
BseXI GCAGC 2 cut(s) 268, 450
BsgI GTGCAG 1 cut(s) 587
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 1 cut(s) 5
BslFI GGGAC 1 cut(s) 162
BsmFI GGGAC 1 cut(s) 162
BsnI GGCC 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 545
Bsp143I GATC 2 cut(s) 85, 715
Bsp68I TCGCGA 1 cut(s) 84
BspACI CCGC 1 cut(s) 244
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 3 cut(s) 20, 136, 654
BspFNI CGCG 1 cut(s) 84
BspMAI CTGCAG 2 cut(s) 383, 468
BspT104I TTCGAA 1 cut(s) 545
BsrDI GCAATG 1 cut(s) 69
BssMI GATC 2 cut(s) 85, 715
BssNI GRCGYC 1 cut(s) 445
BstACI GRCGYC 1 cut(s) 445
BstBI TTCGAA 1 cut(s) 545
BstC8I GCNNGC 2 cut(s) 190, 248
BstDEI CTNAG 3 cut(s) 7, 144, 641
BstF5I GGATG 4 cut(s) 62, 100, 145, 685
BstFNI CGCG 1 cut(s) 84
BstKTI GATC 2 cut(s) 88, 718
BstMBI GATC 2 cut(s) 85, 715
BstMWI GCNNNNNNNGC 2 cut(s) 11, 256
BstNSI RCATGY 1 cut(s) 250
BstSFI CTRYAG 2 cut(s) 379, 464
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 2 cut(s) 268, 450
BstV2I GAAGAC 1 cut(s) 630
BstX2I RGATCY 1 cut(s) 715
BstXI CCANNNNNNTGG 1 cut(s) 315
BstYI RGATCY 1 cut(s) 715
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 4 cut(s) 62, 100, 145, 685
BtsI GCAGTG 1 cut(s) 376
BtsIMutI CAGTG 2 cut(s) 376, 721
BtuMI TCGCGA 1 cut(s) 84
Cac8I GCNNGC 2 cut(s) 190, 248
CaiI CAGNNNCTG 1 cut(s) 112
CseI GACGC 1 cut(s) 434
Csp6I GTAC 3 cut(s) 241, 319, 439
CviAII CATG 5 cut(s) 121, 238, 247, 251, 527
CviJI RGCY 6 cut(s) 5, 11, 112, 343, 403, 463
CviKI_1 RGCY 6 cut(s) 5, 11, 112, 343, 403, 463
CviQI GTAC 3 cut(s) 241, 319, 439
DdeI CTNAG 3 cut(s) 7, 144, 641
DpnI GATC 2 cut(s) 87, 717
DpnII GATC 2 cut(s) 85, 715
EcoRI GAATTC 1 cut(s) 233
EcoT22I ATGCAT 2 cut(s) 252, 416
FaeI CATG 5 cut(s) 124, 241, 250, 254, 530
FalI AAGNNNNNCTT 2 cut(s) 594, 626
FaqI GGGAC 1 cut(s) 162
FatI CATG 5 cut(s) 120, 237, 246, 250, 526
FblI GTMKAC 1 cut(s) 271
Fnu4HI GCNGC 3 cut(s) 12, 257, 464
FokI GGATG 4 cut(s) 49, 107, 152, 692
Fsp4HI GCNGC 3 cut(s) 12, 257, 464
FspBI CTAG 3 cut(s) 134, 275, 705
GluI GCNGC 3 cut(s) 12, 257, 464
HaeIII GGCC 1 cut(s) 5
HgaI GACGC 1 cut(s) 434
Hin1I GRCGYC 1 cut(s) 445
Hin1II CATG 5 cut(s) 124, 241, 250, 254, 530
HincII GTYRAC 1 cut(s) 272
HindII GTYRAC 1 cut(s) 272
HinfI GANTC 2 cut(s) 493, 581
Hpy166II GTNNAC 1 cut(s) 272
Hpy188I TCNGA 2 cut(s) 488, 644
Hpy188III TCNNGA 5 cut(s) 25, 83, 425, 497, 691
Hpy8I GTNNAC 1 cut(s) 272
HpyAV CCTTC 5 cut(s) 49, 139, 161, 169, 527
HpyCH4V TGCA 8 cut(s) 14, 250, 291, 335, 381, 414, 466, 568
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 256
HpyF3I CTNAG 3 cut(s) 7, 144, 641
Hsp92I GRCGYC 1 cut(s) 445
Hsp92II CATG 5 cut(s) 124, 241, 250, 254, 530
Kzo9I GATC 2 cut(s) 85, 715
LmnI GCTCC 1 cut(s) 109
Lsp1109I GCAGC 2 cut(s) 268, 450
LweI GCATC 2 cut(s) 71, 130
MaeI CTAG 3 cut(s) 134, 275, 705
MalI GATC 2 cut(s) 87, 717
MboI GATC 2 cut(s) 85, 715
MboII GAAGA 5 cut(s) 230, 614, 635, 643, 710
MflI RGATCY 1 cut(s) 715
MluCI AATT 4 cut(s) 233, 313, 420, 450
MlyI GAGTC 1 cut(s) 590
MmeI TCCRAC 1 cut(s) 389
MnlI CCTC 8 cut(s) 16, 76, 130, 139, 156, 260, 638, 678
Mph1103I ATGCAT 2 cut(s) 252, 416
MroXI GAANNNNTTC 2 cut(s) 128, 541
MseI TTAA 1 cut(s) 102
MspA1I CMGCKG 1 cut(s) 11
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 2 cut(s) 11, 256
NdeII GATC 2 cut(s) 85, 715
NlaIII CATG 5 cut(s) 124, 241, 250, 254, 530
NruI TCGCGA 1 cut(s) 84
NsiI ATGCAT 2 cut(s) 252, 416
NspI RCATGY 1 cut(s) 250
NspV TTCGAA 1 cut(s) 545
PaeI GCATGC 1 cut(s) 250
PdmI GAANNNNTTC 2 cut(s) 128, 541
PfeI GAWTC 1 cut(s) 493
PkrI GCNGC 3 cut(s) 13, 258, 465
PleI GAGTC 1 cut(s) 589
PpsI GAGTC 1 cut(s) 589
PstI CTGCAG 2 cut(s) 383, 468
PstNI CAGNNNCTG 1 cut(s) 112
PsuI RGATCY 1 cut(s) 715
PvuII CAGCTG 1 cut(s) 11
RruI TCGCGA 1 cut(s) 84
RsaI GTAC 3 cut(s) 242, 320, 440
RsaNI GTAC 3 cut(s) 241, 319, 439
SalI GTCGAC 1 cut(s) 270
SaqAI TTAA 1 cut(s) 102
SatI GCNGC 3 cut(s) 12, 257, 464
Sau3AI GATC 2 cut(s) 85, 715
SchI GAGTC 1 cut(s) 590
SetI ASST 9 cut(s) 13, 114, 271, 284, 341, 401, 465, 544, 649
SfaNI GCATC 2 cut(s) 71, 130
SfcI CTRYAG 2 cut(s) 379, 464
SfuI TTCGAA 1 cut(s) 545
SphI GCATGC 1 cut(s) 250
Sse9I AATT 4 cut(s) 233, 313, 420, 450
SsiI CCGC 1 cut(s) 244
SspMI CTAG 3 cut(s) 134, 275, 705
TaqI TCGA 2 cut(s) 271, 545
TasI AATT 4 cut(s) 233, 313, 420, 450
TfiI GAWTC 1 cut(s) 493
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TscAI CASTG 1 cut(s) 383
TseI GCWGC 3 cut(s) 11, 256, 463
TspDTI ATGAA 5 cut(s) 137, 226, 246, 583, 621
TspRI CASTG 1 cut(s) 383
XapI RAATTY 1 cut(s) 233
XceI RCATGY 1 cut(s) 250
XcmI CCANNNNNNNNNTGG 1 cut(s) 432
XmiI GTMKAC 1 cut(s) 271
XmnI GAANNNNTTC 2 cut(s) 128, 541
XspI CTAG 3 cut(s) 134, 275, 705
Zsp2I ATGCAT 2 cut(s) 252, 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.