Rroxscaffold_2G00126300

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0006386
Physical Location & Seq
Forward (+)
61503119 .. 61525038
21920 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00126300.1

Sequence Viewer

Length: 1017 bp
ATGTGTGCGACAAAGTTGATTGAGGTTTTGAAGGAATATGGGATGACCAAGGCACCGGACCACATCCCACGCTCTCGCCTCGTCCTTGCACGGCTTCCGGTGGCTACCTTGCACCGCAACACGGCCGGCGGCGGCGGTGGAGGACACGATTCCGTTTTGAGCTCGTTTTGGTTCGATCTCGATATCTCGAGCTACAAGCCATCAATCCTCTTGATTTTTGGCTCATTTGATTCGCATAAACTTTCGAACAAGCACCAAGAAGGACCAGACCTGAATCGACCATTTTTACGTTCGTCGGAGTTCGGCCGTGGAAAATCGAAGAAACAAGCTTTCGAATCCGGATATCTCGAGCTGTTTCAAGAACACCACCAGCTAGCTGTACCTCCTGCTATAACACCCTCTGCGAAATTCACCAGAGAAACTAAATCACGAATAAAGTATCCTACAGAATATGGAATGTTTCACTTCTGTATAGCTGACAGAGAACATGACTGGAGGGAGGGAACAGAGAAGTACAAGTTCTTTGAGAATTGCCCTGGATTCTTGGATAGACAAAAGATGAAATTGATTCGAGTTCTGAATCGTAAATTGAAGGGATCTTTTGGGAAAATATATTCATACTTTTCTTTTCTACTCCACTACAACCCAGCTCCCCGAGCTGCTTTTTCTGTCGGCAACCATACACTTCATCTCCTCTTCTTCCGATTCCCTTTCTCTTCCTCGGACCAAGGGAAGGATCGGTCAAACTCATTTCAAATCTGCCATCAAGACTTTGGCGGTGTCCAAGGCGTCCCCAAAAGCAGTTCCTCTTCCTCCGGGCGTTCGCTTCAACCGGGTGGGCTAGAATACTGTACCTCTATTCGGAGTTTGTCAATATCGGCTTGCCCAAGGTACCATCCATTCCAATCGAAAGTCGAGTGCAACCCTCGAAGATCCGTGTGTAAGTACTCTAGACTCTCTTCCAATTTCACGGAGTGGAGGCTATTCCGAGTGGTGGGGAAACCTTGCATCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

338

Amino Acids

38.4

Weight (kDa)

9.72

Isoelectric Point (pI)

52.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 891
AccB1I GGYRCC 2 cut(s) 52, 891
AccIII TCCGGA 1 cut(s) 338
AciI CCGC 5 cut(s) 115, 129, 132, 135, 777
AclWI GGATC 3 cut(s) 604, 744, 927
AcoI YGGCCR 2 cut(s) 123, 304
AcsI RAATTY 1 cut(s) 407
AcyI GRCGYC 1 cut(s) 789
AdeI CACNNNGTG 1 cut(s) 975
AfaI GTAC 5 cut(s) 381, 515, 853, 893, 947
AfiI CCNNNNNNNGG 4 cut(s) 121, 733, 861, 994
AgsI TTSAA 5 cut(s) 31, 359, 592, 755, 830
AjnI CCWGG 1 cut(s) 535
AluBI AGCT 9 cut(s) 162, 192, 329, 352, 373, 377, 476, 650, 659
AluI AGCT 9 cut(s) 162, 192, 329, 352, 373, 377, 476, 650, 659
Alw21I GWGCWC 1 cut(s) 164
AlwI GGATC 3 cut(s) 604, 744, 927
Ama87I CYCGRG 3 cut(s) 187, 347, 654
Aor13HI TCCGGA 1 cut(s) 338
AoxI GGCC 2 cut(s) 123, 304
ApeKI GCWGC 1 cut(s) 659
ApoI RAATTY 1 cut(s) 407
Asp718I GGTACC 1 cut(s) 891
AspS9I GGNCC 3 cut(s) 58, 263, 724
AsuC2I CCSGG 2 cut(s) 817, 834
AsuHPI GGTGA 1 cut(s) 403
AsuII TTCGAA 2 cut(s) 245, 333
AsuNHI GCTAGC 1 cut(s) 373
AvaI CYCGRG 3 cut(s) 187, 347, 654
AvaII GGWCC 3 cut(s) 58, 263, 724
BanI GGYRCC 2 cut(s) 52, 891
BanII GRGCYC 1 cut(s) 164
Bbv12I GWGCWC 1 cut(s) 164
BbvI GCAGC 1 cut(s) 646
BccI CCATC 3 cut(s) 208, 771, 903
BceAI ACGGC 3 cut(s) 107, 138, 291
BciT130I CCWGG 1 cut(s) 537
BciVI GTATCC 1 cut(s) 450
BcnI CCSGG 2 cut(s) 817, 834
BfaI CTAG 3 cut(s) 374, 842, 951
BfmI CTRYAG 1 cut(s) 444
BfuI GTATCC 1 cut(s) 450
BisI GCNGC 3 cut(s) 130, 133, 660
BlsI GCNGC 3 cut(s) 131, 134, 661
BmcAI AGTACT 1 cut(s) 947
Bme1390I CCNGG 3 cut(s) 537, 817, 834
Bme18I GGWCC 3 cut(s) 58, 263, 724
BmeT110I CYCGRG 3 cut(s) 187, 347, 654
BmgT120I GGNCC 3 cut(s) 58, 263, 724
BmiI GGNNCC 2 cut(s) 54, 893
BmrFI CCNGG 3 cut(s) 537, 817, 834
BmtI GCTAGC 1 cut(s) 377
BpmI CTGGAG 1 cut(s) 514
Bpu14I TTCGAA 2 cut(s) 245, 333
BpuMI CCSGG 2 cut(s) 817, 834
BsaHI GRCGYC 1 cut(s) 789
BsaJI CCNNGG 7 cut(s) 48, 307, 535, 720, 727, 784, 887
BsaWI WCCGGW 3 cut(s) 55, 97, 338
BsaXI ACNNNNNCTCC 2 cut(s) 675, 705
Bsc4I CCNNNNNNNGG 4 cut(s) 121, 733, 861, 994
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 1 cut(s) 497
BseAI TCCGGA 1 cut(s) 338
BseBI CCWGG 1 cut(s) 537
BseDI CCNNGG 7 cut(s) 48, 307, 535, 720, 727, 784, 887
BseGI GGATG 3 cut(s) 48, 63, 895
BseLI CCNNNNNNNGG 4 cut(s) 121, 733, 861, 994
BseNI ACTGG 1 cut(s) 497
BseRI GAGGAG 1 cut(s) 683
BseX3I CGGCCG 2 cut(s) 123, 304
BseXI GCAGC 1 cut(s) 646
BseYI CCCAGC 1 cut(s) 646
Bsh1285I CGRYCG 2 cut(s) 126, 307
BshFI GGCC 2 cut(s) 125, 306
BshNI GGYRCC 2 cut(s) 52, 891
BsiEI CGRYCG 2 cut(s) 126, 307
BsiHKAI GWGCWC 1 cut(s) 164
BsiHKCI CYCGRG 3 cut(s) 187, 347, 654
BsiSI CCGG 6 cut(s) 56, 98, 126, 339, 816, 833
BslFI GGGAC 1 cut(s) 776
BslI CCNNNNNNNGG 4 cut(s) 121, 733, 861, 994
BsmFI GGGAC 1 cut(s) 776
BsnI GGCC 2 cut(s) 125, 306
BsoBI CYCGRG 3 cut(s) 187, 347, 654
Bsp119I TTCGAA 2 cut(s) 245, 333
Bsp1286I GDGCHC 1 cut(s) 164
Bsp13I TCCGGA 1 cut(s) 338
Bsp143I GATC 4 cut(s) 175, 596, 736, 932
BspACI CCGC 5 cut(s) 115, 129, 132, 135, 777
BspANI GGCC 2 cut(s) 125, 306
BspEI TCCGGA 1 cut(s) 338
BspLI GGNNCC 2 cut(s) 54, 893
BspOI GCTAGC 1 cut(s) 377
BspPI GGATC 3 cut(s) 604, 744, 927
BspT104I TTCGAA 2 cut(s) 245, 333
BspT107I GGYRCC 2 cut(s) 52, 891
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 1 cut(s) 497
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 7 cut(s) 48, 307, 535, 720, 727, 784, 887
BssMI GATC 4 cut(s) 175, 596, 736, 932
BssNI GRCGYC 1 cut(s) 789
BssT1I CCWWGG 4 cut(s) 48, 727, 784, 887
Bst2UI CCWGG 1 cut(s) 537
Bst4CI ACNGT 1 cut(s) 851
Bst6I CTCTTC 4 cut(s) 701, 721, 814, 964
BstACI GRCGYC 1 cut(s) 789
BstBI TTCGAA 2 cut(s) 245, 333
BstC8I GCNNGC 3 cut(s) 127, 375, 883
BstDSI CCRYGG 1 cut(s) 307
BstF5I GGATG 3 cut(s) 48, 63, 895
BstKTI GATC 4 cut(s) 178, 599, 739, 935
BstMBI GATC 4 cut(s) 175, 596, 736, 932
BstMCI CGRYCG 2 cut(s) 126, 307
BstMWI GCNNNNNNNGC 1 cut(s) 656
BstNI CCWGG 1 cut(s) 537
BstSCI CCNGG 3 cut(s) 535, 815, 832
BstSFI CTRYAG 1 cut(s) 444
BstV1I GCAGC 1 cut(s) 646
BstX2I RGATCY 2 cut(s) 596, 932
BstYI RGATCY 2 cut(s) 596, 932
BstZI CGGCCG 2 cut(s) 123, 304
BsuI GTATCC 1 cut(s) 450
BsuRI GGCC 2 cut(s) 125, 306
BtgI CCRYGG 1 cut(s) 307
BtgZI GCGATG 1 cut(s) 994
BtsCI GGATG 3 cut(s) 48, 63, 895
Cac8I GCNNGC 3 cut(s) 127, 375, 883
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 3 cut(s) 58, 263, 724
CseI GACGC 1 cut(s) 778
Csp6I GTAC 5 cut(s) 380, 514, 852, 892, 946
CviAII CATG 1 cut(s) 488
CviQI GTAC 5 cut(s) 380, 514, 852, 892, 946
DpnI GATC 4 cut(s) 177, 598, 738, 934
DpnII GATC 4 cut(s) 175, 596, 736, 932
DraIII CACNNNGTG 1 cut(s) 975
EaeI YGGCCR 2 cut(s) 123, 304
EagI CGGCCG 2 cut(s) 123, 304
Eam1104I CTCTTC 4 cut(s) 701, 721, 814, 964
EarI CTCTTC 4 cut(s) 701, 721, 814, 964
Ecl136II GAGCTC 1 cut(s) 162
EclXI CGGCCG 2 cut(s) 123, 304
Eco130I CCWWGG 4 cut(s) 48, 727, 784, 887
Eco24I GRGCYC 1 cut(s) 164
Eco32I GATATC 2 cut(s) 184, 344
Eco47I GGWCC 3 cut(s) 58, 263, 724
Eco52I CGGCCG 2 cut(s) 123, 304
Eco53kI GAGCTC 1 cut(s) 162
Eco88I CYCGRG 3 cut(s) 187, 347, 654
EcoICRI GAGCTC 1 cut(s) 162
EcoRII CCWGG 1 cut(s) 535
EcoRV GATATC 2 cut(s) 184, 344
EcoT14I CCWWGG 4 cut(s) 48, 727, 784, 887
EcoT38I GRGCYC 1 cut(s) 164
ErhI CCWWGG 4 cut(s) 48, 727, 784, 887
FaeI CATG 1 cut(s) 491
FaiI YATR 9 cut(s) 39, 237, 392, 453, 473, 489, 613, 619, 681
FaqI GGGAC 1 cut(s) 776
FatI CATG 1 cut(s) 487
Fnu4HI GCNGC 3 cut(s) 130, 133, 660
FokI GGATG 3 cut(s) 50, 55, 882
FriOI GRGCYC 1 cut(s) 164
Fsp4HI GCNGC 3 cut(s) 130, 133, 660
FspBI CTAG 3 cut(s) 374, 842, 951
GluI GCNGC 3 cut(s) 130, 133, 660
GsaI CCCAGC 1 cut(s) 650
GsuI CTGGAG 1 cut(s) 514
HaeIII GGCC 2 cut(s) 125, 306
HapII CCGG 6 cut(s) 56, 98, 126, 339, 816, 833
HgaI GACGC 1 cut(s) 778
Hin1I GRCGYC 1 cut(s) 789
Hin1II CATG 1 cut(s) 491
HindIII AAGCTT 1 cut(s) 327
HinfI GANTC 9 cut(s) 149, 230, 274, 335, 540, 568, 580, 705, 954
HpaII CCGG 6 cut(s) 56, 98, 126, 339, 816, 833
HphI GGTGA 1 cut(s) 403
Hpy188I TCNGA 6 cut(s) 298, 579, 704, 724, 864, 989
Hpy188III TCNNGA 9 cut(s) 179, 187, 211, 339, 347, 359, 429, 767, 951
Hpy99I CGWCG 1 cut(s) 298
HpyAV CCTTC 4 cut(s) 25, 254, 586, 727
HpyCH4III ACNGT 1 cut(s) 851
HpyCH4IV ACGT 1 cut(s) 289
HpyCH4V TGCA 4 cut(s) 89, 112, 921, 1008
HpyF10VI GCNNNNNNNGC 1 cut(s) 656
HpySE526I ACGT 1 cut(s) 289
Hsp92I GRCGYC 1 cut(s) 789
Hsp92II CATG 1 cut(s) 491
Kpn2I TCCGGA 1 cut(s) 338
KpnI GGTACC 1 cut(s) 895
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
Kzo9I GATC 4 cut(s) 175, 596, 736, 932
LmnI GCTCC 1 cut(s) 655
Lsp1109I GCAGC 1 cut(s) 646
MaeI CTAG 3 cut(s) 374, 842, 951
MaeII ACGT 1 cut(s) 289
MalI GATC 4 cut(s) 177, 598, 738, 934
MboI GATC 4 cut(s) 175, 596, 736, 932
MboII GAAGA 7 cut(s) 331, 688, 691, 708, 801, 942, 951
MflI RGATCY 2 cut(s) 596, 932
MhlI GDGCHC 1 cut(s) 164
MluCI AATT 5 cut(s) 407, 529, 563, 587, 964
MlyI GAGTC 1 cut(s) 948
MmeI TCCRAC 1 cut(s) 276
MroI TCCGGA 1 cut(s) 338
MroNI GCCGGC 1 cut(s) 125
MspI CCGG 6 cut(s) 56, 98, 126, 339, 816, 833
MspR9I CCNGG 3 cut(s) 537, 817, 834
MvaI CCWGG 1 cut(s) 537
MwoI GCNNNNNNNGC 1 cut(s) 656
NaeI GCCGGC 1 cut(s) 127
NciI CCSGG 2 cut(s) 817, 834
NdeII GATC 4 cut(s) 175, 596, 736, 932
NgoMIV GCCGGC 1 cut(s) 125
NheI GCTAGC 1 cut(s) 373
NlaIII CATG 1 cut(s) 491
NlaIV GGNNCC 2 cut(s) 54, 893
NspV TTCGAA 2 cut(s) 245, 333
PaeR7I CTCGAG 2 cut(s) 187, 347
PdiI GCCGGC 1 cut(s) 127
PfeI GAWTC 8 cut(s) 149, 230, 274, 335, 540, 568, 580, 705
PkrI GCNGC 3 cut(s) 131, 134, 661
PleI GAGTC 1 cut(s) 948
PpsI GAGTC 1 cut(s) 948
Psp124BI GAGCTC 1 cut(s) 164
Psp6I CCWGG 1 cut(s) 535
PspFI CCCAGC 1 cut(s) 646
PspGI CCWGG 1 cut(s) 535
PspN4I GGNNCC 2 cut(s) 54, 893
PspPI GGNCC 3 cut(s) 58, 263, 724
PsuI RGATCY 2 cut(s) 596, 932
RsaI GTAC 5 cut(s) 381, 515, 853, 893, 947
RsaNI GTAC 5 cut(s) 380, 514, 852, 892, 946
SacI GAGCTC 1 cut(s) 164
SatI GCNGC 3 cut(s) 130, 133, 660
Sau3AI GATC 4 cut(s) 175, 596, 736, 932
Sau96I GGNCC 3 cut(s) 58, 263, 724
ScaI AGTACT 1 cut(s) 947
SchI GAGTC 1 cut(s) 948
ScrFI CCNGG 3 cut(s) 537, 817, 834
SduI GDGCHC 1 cut(s) 164
SfcI CTRYAG 1 cut(s) 444
Sfr274I CTCGAG 2 cut(s) 187, 347
SfuI TTCGAA 2 cut(s) 245, 333
SinI GGWCC 3 cut(s) 58, 263, 724
SlaI CTCGAG 2 cut(s) 187, 347
SmlI CTYRAG 2 cut(s) 187, 347
SmoI CTYRAG 2 cut(s) 187, 347
Sse9I AATT 5 cut(s) 407, 529, 563, 587, 964
SsiI CCGC 5 cut(s) 115, 129, 132, 135, 777
SspMI CTAG 3 cut(s) 374, 842, 951
SstI GAGCTC 1 cut(s) 164
StyD4I CCNGG 3 cut(s) 535, 815, 832
StyI CCWWGG 4 cut(s) 48, 727, 784, 887
TaaI ACNGT 1 cut(s) 851
TaiI ACGT 1 cut(s) 292
TaqII GACCGA 1 cut(s) 729
TasI AATT 5 cut(s) 407, 529, 563, 587, 964
TatI WGTACW 2 cut(s) 513, 945
TauI GCSGC 2 cut(s) 132, 135
TfiI GAWTC 8 cut(s) 149, 230, 274, 335, 540, 568, 580, 705
TseI GCWGC 1 cut(s) 659
TspDTI ATGAA 3 cut(s) 575, 606, 677
TspGWI ACGGA 3 cut(s) 142, 925, 986
VpaK11BI GGWCC 3 cut(s) 58, 263, 724
XapI RAATTY 1 cut(s) 407
XbaI TCTAGA 1 cut(s) 950
XcmI CCANNNNNNNNNTGG 1 cut(s) 770
XhoI CTCGAG 2 cut(s) 187, 347
XspI CTAG 3 cut(s) 374, 842, 951
ZrmI AGTACT 1 cut(s) 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.