Rh4DG365700

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0006386
Physical Location & Seq
Reverse (-)
58650736 .. 58651510
775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG365700.1

Sequence Viewer

Length: 597 bp
ATGTTCCGGTTCTGCATAGCCGACACAGAACATGATTGGAGAGAAGGAACAGAGCAGTACAAGTTCATTGAGAACTGCTTTGCAACAGTTGATAGACAAAAGCAACCATGGCTGATATTTCTTGCACATCGGGTATTGGGTTACTCTTCTGCGAGCTTTTATGTAGCTGAAGGATCGTTTGAGGAACCAATGGGAAGGGAGAGCCTTCAGAAACTATGGCAGAAATACAAGGTTGACATTGCCGTATATGGCCATGTGCACAATTATGAAAGGACATGTCCCATTTACGAGAATATCTGCACTAGCAAGGAGAAGCAAAATTACGTAGGTAATCTGAATGGGACAATCCATGTGGTTGCTGGCGGTGGAGGAGCAAGTCTTGCAACCTTTGCTCCTATTAATACGACATGGAGTATCTTTAAGGACTATGACTATGGATTTGTCAAAATGACTGCATTTGATCACTCAAACCTGTTGTTCGAGTACAAGAAGAGCAGCGATGGAAAGGTTTATGACTCTTTCAGAATATCCAAGAATTACAGAGACATCTTGGCCTGCAGTGTTGATAGTTGCCCATGTACAGCAATGGCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

198

Amino Acids

22.69

Weight (kDa)

6.3

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 10 - 89 7.2e-06 Calcineurin-like phosphoesterase
Metallophos_C PF14008 114 - 173 4.8e-16 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 363
AclWI GGATC 1 cut(s) 181
AcoI YGGCCR 1 cut(s) 250
AcuI CTGAAG 2 cut(s) 189, 191
AfaI GTAC 3 cut(s) 59, 485, 580
AflIII ACRYGT 1 cut(s) 275
AluBI AGCT 2 cut(s) 156, 167
AluI AGCT 2 cut(s) 156, 167
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 1 cut(s) 537
Alw44I GTGCAC 1 cut(s) 257
AlwI GGATC 1 cut(s) 181
AoxI GGCC 2 cut(s) 250, 552
ApaLI GTGCAC 1 cut(s) 257
ApeKI GCWGC 1 cut(s) 495
ArsI GACNNNNNNTTYG 2 cut(s) 422, 454
AseI ATTAAT 1 cut(s) 399
BaeGI GKGCMC 1 cut(s) 261
BalI TGGCCA 1 cut(s) 252
BarI GAAGNNNNNNTAC 2 cut(s) 305, 337
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 1 cut(s) 507
BccI CCATC 1 cut(s) 494
BceAI ACGGC 1 cut(s) 227
BclI TGATCA 1 cut(s) 460
BcoDI GTCTC 1 cut(s) 537
BfaI CTAG 1 cut(s) 303
BfmI CTRYAG 1 cut(s) 556
BisI GCNGC 1 cut(s) 496
BlsI GCNGC 1 cut(s) 497
BmiI GGNNCC 1 cut(s) 186
BsaAI YACGTR 1 cut(s) 325
BsaJI CCNNGG 1 cut(s) 107
BsaWI WCCGGW 1 cut(s) 6
BsaXI ACNNNNNCTCC 2 cut(s) 376, 406
Bse3DI GCAATG 2 cut(s) 237, 591
BseDI CCNNGG 1 cut(s) 107
BseMI GCAATG 2 cut(s) 237, 591
BseRI GAGGAG 1 cut(s) 384
BseSI GKGCMC 1 cut(s) 261
BseXI GCAGC 1 cut(s) 507
BsgI GTGCAG 1 cut(s) 283
BshFI GGCC 2 cut(s) 252, 554
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 1 cut(s) 7
BslFI GGGAC 2 cut(s) 264, 355
BsmAI GTCTC 1 cut(s) 537
BsmFI GGGAC 2 cut(s) 264, 355
BsnI GGCC 2 cut(s) 252, 554
Bsp1286I GDGCHC 1 cut(s) 261
Bsp1407I TGTACA 1 cut(s) 578
Bsp143I GATC 2 cut(s) 173, 460
Bsp19I CCATGG 1 cut(s) 107
BspACI CCGC 1 cut(s) 363
BspANI GGCC 2 cut(s) 252, 554
BspLI GGNNCC 1 cut(s) 186
BspMAI CTGCAG 1 cut(s) 560
BspPI GGATC 1 cut(s) 181
BspQI GCTCTTC 1 cut(s) 485
BsrDI GCAATG 2 cut(s) 237, 591
BsrGI TGTACA 1 cut(s) 578
BssECI CCNNGG 1 cut(s) 107
BssMI GATC 2 cut(s) 173, 460
BssT1I CCWWGG 1 cut(s) 107
Bst4CI ACNGT 1 cut(s) 88
Bst6I CTCTTC 2 cut(s) 151, 485
BstAPI GCANNNNNTGC 2 cut(s) 380, 389
BstAUI TGTACA 1 cut(s) 578
BstBAI YACGTR 1 cut(s) 325
BstC8I GCNNGC 3 cut(s) 154, 361, 556
BstDSI CCRYGG 1 cut(s) 107
BstKTI GATC 2 cut(s) 176, 463
BstMAI GTCTC 1 cut(s) 537
BstMBI GATC 2 cut(s) 173, 460
BstMWI GCNNNNNNNGC 3 cut(s) 109, 380, 389
BstNSI RCATGY 1 cut(s) 279
BstSFI CTRYAG 1 cut(s) 556
BstSLI GKGCMC 1 cut(s) 261
BstSNI TACGTA 1 cut(s) 325
BstV1I GCAGC 1 cut(s) 507
BsuRI GGCC 2 cut(s) 252, 554
BtgI CCRYGG 1 cut(s) 107
BtgZI GCGATG 1 cut(s) 513
BtsI GCAGTG 1 cut(s) 565
BtsIMutI CAGTG 1 cut(s) 565
Cac8I GCNNGC 3 cut(s) 154, 361, 556
Csp6I GTAC 3 cut(s) 58, 484, 579
CspCI CAANNNNNGTGG 2 cut(s) 333, 368
CviAII CATG 7 cut(s) 32, 108, 254, 276, 350, 408, 576
CviJI RGCY 7 cut(s) 20, 112, 156, 167, 204, 252, 554
CviKI_1 RGCY 7 cut(s) 20, 112, 156, 167, 204, 252, 554
CviQI GTAC 3 cut(s) 58, 484, 579
DpnI GATC 2 cut(s) 175, 462
DpnII GATC 2 cut(s) 173, 460
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 2 cut(s) 151, 485
EarI CTCTTC 2 cut(s) 151, 485
Eco105I TACGTA 1 cut(s) 325
Eco130I CCWWGG 1 cut(s) 107
Eco57I CTGAAG 2 cut(s) 189, 191
EcoT14I CCWWGG 1 cut(s) 107
ErhI CCWWGG 1 cut(s) 107
FaeI CATG 7 cut(s) 35, 111, 257, 279, 353, 411, 579
FaqI GGGAC 2 cut(s) 264, 355
FatI CATG 7 cut(s) 31, 107, 253, 275, 349, 407, 575
FbaI TGATCA 1 cut(s) 460
Fnu4HI GCNGC 1 cut(s) 496
Fsp4HI GCNGC 1 cut(s) 496
FspBI CTAG 1 cut(s) 303
GluI GCNGC 1 cut(s) 496
HaeIII GGCC 2 cut(s) 252, 554
HapII CCGG 1 cut(s) 7
Hin1II CATG 7 cut(s) 35, 111, 257, 279, 353, 411, 579
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HinfI GANTC 1 cut(s) 515
HpaII CCGG 1 cut(s) 7
Hpy166II GTNNAC 2 cut(s) 235, 259
Hpy188I TCNGA 3 cut(s) 210, 336, 524
Hpy188III TCNNGA 1 cut(s) 594
Hpy8I GTNNAC 2 cut(s) 235, 259
HpyAV CCTTC 4 cut(s) 38, 164, 189, 215
HpyCH4III ACNGT 1 cut(s) 88
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 8 cut(s) 15, 83, 125, 259, 300, 383, 455, 558
HpyF10VI GCNNNNNNNGC 3 cut(s) 109, 380, 389
HpySE526I ACGT 1 cut(s) 324
Hsp92II CATG 7 cut(s) 35, 111, 257, 279, 353, 411, 579
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 2 cut(s) 173, 460
LguI GCTCTTC 1 cut(s) 485
LmnI GCTCC 2 cut(s) 371, 397
LpnPI CCDG 4 cut(s) 20, 345, 485, 568
Lsp1109I GCAGC 1 cut(s) 507
MaeI CTAG 1 cut(s) 303
MaeII ACGT 1 cut(s) 324
MaeIII GTNAC 1 cut(s) 140
MalI GATC 2 cut(s) 175, 462
MboI GATC 2 cut(s) 173, 460
MboII GAAGA 2 cut(s) 138, 502
MhlI GDGCHC 1 cut(s) 261
MlsI TGGCCA 1 cut(s) 252
MluCI AATT 3 cut(s) 262, 319, 535
MluNI TGGCCA 1 cut(s) 252
MlyI GAGTC 1 cut(s) 509
MnlI CCTC 2 cut(s) 175, 362
Mox20I TGGCCA 1 cut(s) 252
MscI TGGCCA 1 cut(s) 252
MseI TTAA 2 cut(s) 399, 420
MslI CAYNNNNRTG 1 cut(s) 264
Msp20I TGGCCA 1 cut(s) 252
MspI CCGG 1 cut(s) 7
MwoI GCNNNNNNNGC 3 cut(s) 109, 380, 389
NcoI CCATGG 1 cut(s) 107
NdeII GATC 2 cut(s) 173, 460
NlaIII CATG 7 cut(s) 35, 111, 257, 279, 353, 411, 579
NlaIV GGNNCC 1 cut(s) 186
NspI RCATGY 1 cut(s) 279
PciI ACATGT 1 cut(s) 275
PciSI GCTCTTC 1 cut(s) 485
PkrI GCNGC 1 cut(s) 497
PleI GAGTC 1 cut(s) 509
PpsI GAGTC 1 cut(s) 509
Ppu21I YACGTR 1 cut(s) 325
PscI ACATGT 1 cut(s) 275
PshBI ATTAAT 1 cut(s) 399
PspN4I GGNNCC 1 cut(s) 186
PstI CTGCAG 1 cut(s) 560
RsaI GTAC 3 cut(s) 59, 485, 580
RsaNI GTAC 3 cut(s) 58, 484, 579
RseI CAYNNNNRTG 1 cut(s) 264
SapI GCTCTTC 1 cut(s) 485
SaqAI TTAA 2 cut(s) 399, 420
SatI GCNGC 1 cut(s) 496
Sau3AI GATC 2 cut(s) 173, 460
SchI GAGTC 1 cut(s) 509
SduI GDGCHC 1 cut(s) 261
SetI ASST 8 cut(s) 158, 169, 234, 327, 331, 389, 474, 510
SfcI CTRYAG 1 cut(s) 556
SmiMI CAYNNNNRTG 1 cut(s) 264
SnaBI TACGTA 1 cut(s) 325
Sse9I AATT 3 cut(s) 262, 319, 535
SsiI CCGC 1 cut(s) 363
SspMI CTAG 1 cut(s) 303
StyI CCWWGG 1 cut(s) 107
TaaI ACNGT 1 cut(s) 88
TaiI ACGT 1 cut(s) 327
TaqI TCGA 1 cut(s) 480
TasI AATT 3 cut(s) 262, 319, 535
TatI WGTACW 3 cut(s) 57, 483, 578
Tru1I TTAA 2 cut(s) 399, 420
Tru9I TTAA 2 cut(s) 399, 420
TscAI CASTG 1 cut(s) 565
TseI GCWGC 1 cut(s) 495
TspDTI ATGAA 2 cut(s) 55, 282
TspRI CASTG 1 cut(s) 565
VneI GTGCAC 1 cut(s) 257
VspI ATTAAT 1 cut(s) 399
XceI RCATGY 1 cut(s) 279
XcmI CCANNNNNNNNNTGG 1 cut(s) 356
XspI CTAG 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.