FvH4_2g15441

Pre-mRNA-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002812
Physical Location & Seq
Reverse (-)
13588702 .. 13592910
4209 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g15441.t2

Sequence Viewer

Length: 933 bp
ATGTCTGATGAAGGAAGAGTGCACCCGGATTGCCGGAATGCATCCAATCCCTACCATGAATGCAGCGAGTATTGCTTCAAAGTTATTGCTGAAACTAAAGCTCGGATGGATCAGTCTCAGTCAGAATCTGTACAAGTTAGTGGGGGTAGTGGCAAATCAAAAGTAGAGGCTGCTCAGCAAGTGGAAGATCTAGATAGACCATCAGAGACTGATGAACATGCTGGTGATGGTGATGAATTTCCCGTTGAGGAGGAGGAGAATATGGAAGGAGACTTCACCAAGTTCACAGGAAGGAAGAAAAGGTTGTGGGAGCTAAGGATGAAGATGAATAAGGCTAGAAAAGCAAATCAGACAGATATTGCAGCTGAAAAGAAAAGAATGGAACCTCCACAAGAGTCAAGGGGTATTTCTAAACAAAAATGGCTTGAAGAGAGGAAAAAAAGAATTGGGAAACTTCTAGATGCAAACGGTTTGGATATGAAAAGCGCTTACATGCTAGATACACAAGAGATGGCAGAGGCAAAGTACAAGAAATGGGAAAAGGATCCTGCCCCATATGGCTGGGATGTTTTTAATCAGAAAACATTGTACGATGCATACAAAAAGCGAACAAAAAATATCGAGGTTGACCTGGAAGAATACAATAAGATGAAAGAAGAAGATCCGGAGTTCTACCGTGAAGCTTCCAGCCTTCAATATGGGAAGGCGCCAAAGATCTCGGAGGATAAGATTGACAAGATGGTGAAGGAACTAAAGGACCAGGAAGAGAAGAAAAAGAAATTTAGCAGGAGGAGGAGGTTCCACGATGAGAAGGATATTGATTCAATCAATGACCGAAATGAGCATTTTAACAAGAAGATTGAGCGAGCCTTTGGCAAATACACATTGGAAATCAAGAACAATCTTGAGCGTGGAACTGCTCTGCCTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
SYF2

Protein Analysis

311

Amino Acids

36.41

Weight (kDa)

6.34

Isoelectric Point (pI)

58.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SYF2 PF08231 156 - 303 9.4e-38 SYF2 splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 706
AccIII TCCGGA 1 cut(s) 664
AclWI GGATC 4 cut(s) 117, 539, 552, 656
AcsI RAATTY 2 cut(s) 236, 779
AcyI GRCGYC 1 cut(s) 707
AfaI GTAC 3 cut(s) 132, 527, 590
AfeI AGCGCT 1 cut(s) 487
AgsI TTSAA 4 cut(s) 79, 428, 695, 825
AjnI CCWGG 2 cut(s) 630, 759
AluBI AGCT 4 cut(s) 101, 313, 365, 683
AluI AGCT 4 cut(s) 101, 313, 365, 683
Alw21I GWGCWC 1 cut(s) 24
Alw26I GTCTC 3 cut(s) 120, 200, 264
Alw44I GTGCAC 1 cut(s) 20
AlwI GGATC 4 cut(s) 117, 539, 552, 656
AlwNI CAGNNNCTG 2 cut(s) 128, 209
Aor13HI TCCGGA 1 cut(s) 664
Aor51HI AGCGCT 1 cut(s) 487
ApaLI GTGCAC 1 cut(s) 20
ApeKI GCWGC 3 cut(s) 63, 170, 362
ApoI RAATTY 2 cut(s) 236, 779
AspLEI GCGC 2 cut(s) 488, 709
AspS9I GGNCC 1 cut(s) 757
AsuC2I CCSGG 1 cut(s) 26
AsuHPI GGTGA 4 cut(s) 236, 242, 268, 754
AvaII GGWCC 1 cut(s) 757
BaeGI GKGCMC 1 cut(s) 24
BamHI GGATCC 1 cut(s) 544
BanI GGYRCC 1 cut(s) 706
Bbv12I GWGCWC 1 cut(s) 24
BbvI GCAGC 3 cut(s) 75, 157, 374
BccI CCATC 5 cut(s) 100, 208, 221, 505, 733
BciT130I CCWGG 2 cut(s) 632, 761
BcnI CCSGG 1 cut(s) 26
BcoDI GTCTC 3 cut(s) 120, 200, 264
BfaI CTAG 4 cut(s) 191, 336, 458, 497
BfoI RGCGCY 2 cut(s) 489, 710
BglII AGATCT 2 cut(s) 187, 714
BisI GCNGC 3 cut(s) 64, 171, 363
BlpI GCTNAGC 1 cut(s) 174
BlsI GCNGC 3 cut(s) 65, 172, 364
Bme1390I CCNGG 3 cut(s) 26, 632, 761
Bme18I GGWCC 1 cut(s) 757
BmgT120I GGNCC 1 cut(s) 757
BmiI GGNNCC 4 cut(s) 384, 546, 708, 800
BmrFI CCNGG 3 cut(s) 26, 632, 761
BmsI GCATC 3 cut(s) 50, 451, 583
Bpu10I CCTNAGC 1 cut(s) 314
Bpu1102I GCTNAGC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 926
BpuMI CCSGG 1 cut(s) 26
BsaHI GRCGYC 1 cut(s) 707
BsaWI WCCGGW 1 cut(s) 664
BseAI TCCGGA 1 cut(s) 664
BseBI CCWGG 2 cut(s) 632, 761
BseGI GGATG 4 cut(s) 41, 111, 324, 571
BseMII CTCAG 2 cut(s) 131, 188
BseRI GAGGAG 5 cut(s) 263, 266, 269, 805, 808
BseSI GKGCMC 1 cut(s) 24
BseXI GCAGC 3 cut(s) 75, 157, 374
BseYI CCCAGC 1 cut(s) 561
BshNI GGYRCC 1 cut(s) 706
BsiHKAI GWGCWC 1 cut(s) 24
BsiSI CCGG 3 cut(s) 26, 34, 665
BsmAI GTCTC 3 cut(s) 120, 200, 264
BsmI GAATGC 2 cut(s) 43, 65
Bsp1286I GDGCHC 1 cut(s) 24
Bsp13I TCCGGA 1 cut(s) 664
Bsp1407I TGTACA 1 cut(s) 130
Bsp143I GATC 5 cut(s) 109, 187, 544, 661, 714
Bsp1720I GCTNAGC 1 cut(s) 174
BspCNI CTCAG 2 cut(s) 130, 187
BspEI TCCGGA 1 cut(s) 664
BspLI GGNNCC 4 cut(s) 384, 546, 708, 800
BspPI GGATC 4 cut(s) 117, 539, 552, 656
BspT107I GGYRCC 1 cut(s) 706
BsrGI TGTACA 1 cut(s) 130
BssMI GATC 5 cut(s) 109, 187, 544, 661, 714
BssNI GRCGYC 1 cut(s) 707
Bst2UI CCWGG 2 cut(s) 632, 761
Bst4CI ACNGT 2 cut(s) 470, 677
Bst6I CTCTTC 3 cut(s) 10, 423, 759
BstACI GRCGYC 1 cut(s) 707
BstAUI TGTACA 1 cut(s) 130
BstC8I GCNNGC 1 cut(s) 867
BstDEI CTNAG 3 cut(s) 117, 174, 314
BstF5I GGATG 4 cut(s) 41, 111, 324, 571
BstH2I RGCGCY 2 cut(s) 489, 710
BstHHI GCGC 2 cut(s) 488, 709
BstKTI GATC 5 cut(s) 112, 190, 547, 664, 717
BstMAI GTCTC 3 cut(s) 120, 200, 264
BstMBI GATC 5 cut(s) 109, 187, 544, 661, 714
BstMWI GCNNNNNNNGC 2 cut(s) 72, 341
BstNI CCWGG 2 cut(s) 632, 761
BstNSI RCATGY 2 cut(s) 221, 496
BstSCI CCNGG 3 cut(s) 24, 630, 759
BstSLI GKGCMC 1 cut(s) 24
BstV1I GCAGC 3 cut(s) 75, 157, 374
BstX2I RGATCY 4 cut(s) 187, 544, 661, 714
BstXI CCANNNNNNTGG 1 cut(s) 561
BstYI RGATCY 4 cut(s) 187, 544, 661, 714
BtsCI GGATG 4 cut(s) 41, 111, 324, 571
Cac8I GCNNGC 1 cut(s) 867
CaiI CAGNNNCTG 2 cut(s) 128, 209
CfoI GCGC 2 cut(s) 488, 709
Cfr13I GGNCC 1 cut(s) 757
Csp6I GTAC 3 cut(s) 131, 526, 589
CviAII CATG 3 cut(s) 56, 218, 493
CviQI GTAC 3 cut(s) 131, 526, 589
DdeI CTNAG 3 cut(s) 117, 174, 314
DinI GGCGCC 1 cut(s) 708
DpnI GATC 5 cut(s) 111, 189, 546, 663, 716
DpnII GATC 5 cut(s) 109, 187, 544, 661, 714
Eam1104I CTCTTC 3 cut(s) 10, 423, 759
EarI CTCTTC 3 cut(s) 10, 423, 759
Eco47I GGWCC 1 cut(s) 757
Eco47III AGCGCT 1 cut(s) 487
EcoRII CCWGG 2 cut(s) 630, 759
EcoT22I ATGCAT 2 cut(s) 43, 598
EgeI GGCGCC 1 cut(s) 708
EheI GGCGCC 1 cut(s) 708
FaeI CATG 3 cut(s) 59, 221, 496
FaiI YATR 9 cut(s) 57, 219, 263, 479, 494, 556, 558, 598, 699
FatI CATG 3 cut(s) 55, 217, 492
FauNDI CATATG 1 cut(s) 556
Fnu4HI GCNGC 3 cut(s) 64, 171, 363
FokI GGATG 4 cut(s) 28, 118, 331, 578
Fsp4HI GCNGC 3 cut(s) 64, 171, 363
FspBI CTAG 4 cut(s) 191, 336, 458, 497
GlaI GCGC 2 cut(s) 487, 708
GluI GCNGC 3 cut(s) 64, 171, 363
GsaI CCCAGC 1 cut(s) 565
HaeII RGCGCY 2 cut(s) 489, 710
HapII CCGG 3 cut(s) 26, 34, 665
HhaI GCGC 2 cut(s) 488, 709
Hin1I GRCGYC 1 cut(s) 707
Hin1II CATG 3 cut(s) 59, 221, 496
Hin6I GCGC 2 cut(s) 486, 707
HinP1I GCGC 2 cut(s) 486, 707
HincII GTYRAC 1 cut(s) 628
HindII GTYRAC 1 cut(s) 628
HindIII AAGCTT 1 cut(s) 681
HinfI GANTC 3 cut(s) 125, 395, 821
HpaII CCGG 3 cut(s) 26, 34, 665
HphI GGTGA 4 cut(s) 236, 242, 268, 754
Hpy166II GTNNAC 3 cut(s) 22, 285, 628
Hpy188I TCNGA 7 cut(s) 7, 105, 124, 205, 351, 579, 721
Hpy188III TCNNGA 5 cut(s) 191, 458, 665, 895, 905
Hpy8I GTNNAC 3 cut(s) 22, 285, 628
HpyAV CCTTC 7 cut(s) 5, 260, 285, 697, 701, 739, 805
HpyCH4III ACNGT 2 cut(s) 470, 677
HpyCH4V TGCA 6 cut(s) 22, 41, 63, 362, 464, 596
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 341
HpyF3I CTNAG 3 cut(s) 117, 174, 314
Hsp92I GRCGYC 1 cut(s) 707
Hsp92II CATG 3 cut(s) 59, 221, 496
HspAI GCGC 2 cut(s) 486, 707
KasI GGCGCC 1 cut(s) 706
Kpn2I TCCGGA 1 cut(s) 664
Kzo9I GATC 5 cut(s) 109, 187, 544, 661, 714
LmnI GCTCC 1 cut(s) 310
Lsp1109I GCAGC 3 cut(s) 75, 157, 374
LweI GCATC 3 cut(s) 50, 451, 583
MaeI CTAG 4 cut(s) 191, 336, 458, 497
MalI GATC 5 cut(s) 111, 189, 546, 663, 716
MboI GATC 5 cut(s) 109, 187, 544, 661, 714
MflI RGATCY 4 cut(s) 187, 544, 661, 714
MhlI GDGCHC 1 cut(s) 24
MluCI AATT 3 cut(s) 236, 444, 779
Mly113I GGCGCC 1 cut(s) 707
MlyI GAGTC 1 cut(s) 404
Mph1103I ATGCAT 2 cut(s) 43, 598
MroI TCCGGA 1 cut(s) 664
MseI TTAA 2 cut(s) 573, 849
MslI CAYNNNNRTG 1 cut(s) 222
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 3 cut(s) 26, 34, 665
MspR9I CCNGG 3 cut(s) 26, 632, 761
Mva1269I GAATGC 2 cut(s) 43, 65
MvaI CCWGG 2 cut(s) 632, 761
MwoI GCNNNNNNNGC 2 cut(s) 72, 341
NarI GGCGCC 1 cut(s) 707
NciI CCSGG 1 cut(s) 26
NdeI CATATG 1 cut(s) 556
NdeII GATC 5 cut(s) 109, 187, 544, 661, 714
NlaIII CATG 3 cut(s) 59, 221, 496
NlaIV GGNNCC 4 cut(s) 384, 546, 708, 800
NsiI ATGCAT 2 cut(s) 43, 598
NspI RCATGY 2 cut(s) 221, 496
PctI GAATGC 2 cut(s) 43, 65
PfeI GAWTC 2 cut(s) 125, 821
PkrI GCNGC 3 cut(s) 65, 172, 364
PleI GAGTC 1 cut(s) 403
PluTI GGCGCC 1 cut(s) 710
PpsI GAGTC 1 cut(s) 403
Psp6I CCWGG 2 cut(s) 630, 759
PspFI CCCAGC 1 cut(s) 561
PspGI CCWGG 2 cut(s) 630, 759
PspN4I GGNNCC 4 cut(s) 384, 546, 708, 800
PspPI GGNCC 1 cut(s) 757
PstNI CAGNNNCTG 2 cut(s) 128, 209
PsuI RGATCY 4 cut(s) 187, 544, 661, 714
PvuII CAGCTG 1 cut(s) 365
RsaI GTAC 3 cut(s) 132, 527, 590
RsaNI GTAC 3 cut(s) 131, 526, 589
RseI CAYNNNNRTG 1 cut(s) 222
SaqAI TTAA 2 cut(s) 573, 849
SatI GCNGC 3 cut(s) 64, 171, 363
Sau3AI GATC 5 cut(s) 109, 187, 544, 661, 714
Sau96I GGNCC 1 cut(s) 757
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 3 cut(s) 26, 632, 761
SduI GDGCHC 1 cut(s) 24
SetI ASST 9 cut(s) 103, 305, 315, 367, 388, 627, 633, 685, 800
SfaNI GCATC 3 cut(s) 50, 451, 583
SfoI GGCGCC 1 cut(s) 708
SinI GGWCC 1 cut(s) 757
SmiMI CAYNNNNRTG 1 cut(s) 222
SmlI CTYRAG 1 cut(s) 905
SmoI CTYRAG 1 cut(s) 905
Sse9I AATT 3 cut(s) 236, 444, 779
SspDI GGCGCC 1 cut(s) 706
SspMI CTAG 4 cut(s) 191, 336, 458, 497
StyD4I CCNGG 3 cut(s) 24, 630, 759
TaaI ACNGT 2 cut(s) 470, 677
TaqI TCGA 1 cut(s) 621
TaqII GACCGA 1 cut(s) 849
TasI AATT 3 cut(s) 236, 444, 779
TatI WGTACW 2 cut(s) 130, 525
TfiI GAWTC 2 cut(s) 125, 821
Tru1I TTAA 2 cut(s) 573, 849
Tru9I TTAA 2 cut(s) 573, 849
TseI GCWGC 3 cut(s) 63, 170, 362
TspDTI ATGAA 8 cut(s) 24, 72, 228, 249, 335, 341, 494, 665
VneI GTGCAC 1 cut(s) 20
VpaK11BI GGWCC 1 cut(s) 757
XapI RAATTY 2 cut(s) 236, 779
XbaI TCTAGA 2 cut(s) 190, 457
XceI RCATGY 2 cut(s) 221, 496
XspI CTAG 4 cut(s) 191, 336, 458, 497
Zsp2I ATGCAT 2 cut(s) 43, 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.