RLG00000013230

Pre-mRNA-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002812
Physical Location & Seq
Forward (+)
28039121 .. 28041317
2197 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013230

Sequence Viewer

Length: 939 bp
ATGTCTGATGAAGGAAGAGTGCACCCGGATTGCAGGAATGCATCCAATCCCTACCACGAATGCAGCGAGTATTGCTTCAAAGTTATTGCTGAAACCAAAGCTCGGATGGATCAAAATCAATCAGAATCTGTACAAGTTAGTGGTGGTAGTGGCAAATCAAAATTAGAGGCTGCTCAGCAAGAGGGAGATCTAGATAGACCATCAGAGACTGATGAACATGCTGGTGATAGTGATGGTGATGAGCCTCCTGTTGAGGAGGAGGAGAATATGGAAGGAGACTTCACAAAGTTCACAGGGAGGAAGAAAAAGTTGTGGGAATTAAGGATGAAGATGAATAAGGCTAGGAAAGCAAATCAAACAGATATTGCAGCTGAAAAGAAAAGAATGGAACCTCCACAAGAATCAAGGGGCATTTCTAAACAAAAATGGCTTGAAGAGAGGAAAAAAAGAATTGGTAAACTTCTAGATGCAAATGGTTTGGATATGAAAACTGCTTACATGCTAGATACACAAGAGATGGCAGAGTCAAAGTACAAGAAATGGGAAAAGGATCCTGCCCCATATGGCTGGGATGTTTTTAATCAGAAAACATTGTATGATGCATACAAAAAGCGAACAAAGAATATTGATATTGACTTGGAAGATTACAATAAGATGAAAGAAGAAGATCCAGAGTTCTACCGTGAAGCTTCCAGCCTTCAATACGGGAAGGCTCCAAAGATCTCAGAGGATAAAATTGACAAGATGGTGAAGGAACTAAAGGACCAGGAAGAGAAGAAAAAGAAATTCAGCAGGAGGAGGAGGTTCCATGATGAGAAGGATATTGACTCGATCAATGACCGTAATGAGCATTTTAACAAGAAGATTGAGCGAGCTTTTGGTAAATACACGCTGGAAATCAAAAACAATCTCGAGCGAGGAACTGCATTGCCCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
SYF2

Protein Analysis

313

Amino Acids

36.55

Weight (kDa)

6.13

Isoelectric Point (pI)

55.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SYF2 PF08231 158 - 305 1.6e-37 SYF2 splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 117, 545, 558, 662
AcsI RAATTY 1 cut(s) 785
AfaI GTAC 2 cut(s) 132, 533
AfiI CCNNNNNNNGG 1 cut(s) 102
AgsI TTSAA 3 cut(s) 79, 434, 701
AjnI CCWGG 1 cut(s) 765
AluBI AGCT 4 cut(s) 101, 371, 689, 875
AluI AGCT 4 cut(s) 101, 371, 689, 875
Alw21I GWGCWC 1 cut(s) 24
Alw26I GTCTC 2 cut(s) 200, 270
Alw44I GTGCAC 1 cut(s) 20
AlwI GGATC 4 cut(s) 117, 545, 558, 662
AlwNI CAGNNNCTG 2 cut(s) 128, 209
Ama87I CYCGRG 1 cut(s) 911
ApaLI GTGCAC 1 cut(s) 20
ApeKI GCWGC 3 cut(s) 63, 170, 368
ApoI RAATTY 1 cut(s) 785
AspS9I GGNCC 1 cut(s) 763
AsuC2I CCSGG 1 cut(s) 26
AsuHPI GGTGA 3 cut(s) 236, 248, 760
AvaI CYCGRG 1 cut(s) 911
AvaII GGWCC 1 cut(s) 763
BaeGI GKGCMC 1 cut(s) 24
BamHI GGATCC 1 cut(s) 550
Bbv12I GWGCWC 1 cut(s) 24
BbvI GCAGC 3 cut(s) 75, 157, 380
BccI CCATC 5 cut(s) 100, 208, 227, 511, 739
BciT130I CCWGG 1 cut(s) 767
BcnI CCSGG 1 cut(s) 26
BcoDI GTCTC 2 cut(s) 200, 270
BfaI CTAG 4 cut(s) 191, 342, 464, 503
BglII AGATCT 2 cut(s) 187, 720
BisI GCNGC 3 cut(s) 64, 171, 369
BlpI GCTNAGC 1 cut(s) 174
BlsI GCNGC 3 cut(s) 65, 172, 370
Bme1390I CCNGG 2 cut(s) 26, 767
Bme18I GGWCC 1 cut(s) 763
BmeT110I CYCGRG 1 cut(s) 911
BmgT120I GGNCC 1 cut(s) 763
BmiI GGNNCC 4 cut(s) 390, 552, 714, 806
BmrFI CCNGG 2 cut(s) 26, 767
BmsI GCATC 3 cut(s) 50, 457, 589
Bpu1102I GCTNAGC 1 cut(s) 174
BpuMI CCSGG 1 cut(s) 26
BsaBI GATNNNNATC 1 cut(s) 114
Bsc4I CCNNNNNNNGG 1 cut(s) 102
Bse3DI GCAATG 1 cut(s) 926
Bse8I GATNNNNATC 1 cut(s) 114
BseBI CCWGG 1 cut(s) 767
BseGI GGATG 4 cut(s) 41, 111, 330, 577
BseJI GATNNNNATC 1 cut(s) 114
BseLI CCNNNNNNNGG 1 cut(s) 102
BseMI GCAATG 1 cut(s) 926
BseMII CTCAG 2 cut(s) 188, 738
BseRI GAGGAG 5 cut(s) 269, 272, 275, 811, 814
BseSI GKGCMC 1 cut(s) 24
BseXI GCAGC 3 cut(s) 75, 157, 380
BseYI CCCAGC 1 cut(s) 567
BsiHKAI GWGCWC 1 cut(s) 24
BsiHKCI CYCGRG 1 cut(s) 911
BsiSI CCGG 1 cut(s) 26
BslI CCNNNNNNNGG 1 cut(s) 102
BsmAI GTCTC 2 cut(s) 200, 270
BsmI GAATGC 2 cut(s) 43, 65
BsoBI CYCGRG 1 cut(s) 911
Bsp1286I GDGCHC 1 cut(s) 24
Bsp1407I TGTACA 1 cut(s) 130
Bsp143I GATC 6 cut(s) 109, 187, 550, 667, 720, 831
Bsp1720I GCTNAGC 1 cut(s) 174
BspCNI CTCAG 2 cut(s) 187, 737
BspLI GGNNCC 4 cut(s) 390, 552, 714, 806
BspPI GGATC 4 cut(s) 117, 545, 558, 662
BsrDI GCAATG 1 cut(s) 926
BsrGI TGTACA 1 cut(s) 130
BssMI GATC 6 cut(s) 109, 187, 550, 667, 720, 831
Bst2UI CCWGG 1 cut(s) 767
Bst4CI ACNGT 2 cut(s) 683, 842
Bst6I CTCTTC 3 cut(s) 10, 429, 765
BstAUI TGTACA 1 cut(s) 130
BstC8I GCNNGC 1 cut(s) 873
BstDEI CTNAG 2 cut(s) 174, 724
BstF5I GGATG 4 cut(s) 41, 111, 330, 577
BstKTI GATC 6 cut(s) 112, 190, 553, 670, 723, 834
BstMAI GTCTC 2 cut(s) 200, 270
BstMBI GATC 6 cut(s) 109, 187, 550, 667, 720, 831
BstMWI GCNNNNNNNGC 2 cut(s) 72, 347
BstNI CCWGG 1 cut(s) 767
BstNSI RCATGY 2 cut(s) 221, 502
BstSCI CCNGG 2 cut(s) 24, 765
BstSLI GKGCMC 1 cut(s) 24
BstV1I GCAGC 3 cut(s) 75, 157, 380
BstX2I RGATCY 4 cut(s) 187, 550, 667, 720
BstXI CCANNNNNNTGG 1 cut(s) 567
BstYI RGATCY 4 cut(s) 187, 550, 667, 720
BtsCI GGATG 4 cut(s) 41, 111, 330, 577
Cac8I GCNNGC 1 cut(s) 873
CaiI CAGNNNCTG 2 cut(s) 128, 209
Cfr13I GGNCC 1 cut(s) 763
Csp6I GTAC 2 cut(s) 131, 532
CviAII CATG 3 cut(s) 218, 499, 809
CviQI GTAC 2 cut(s) 131, 532
DdeI CTNAG 2 cut(s) 174, 724
DpnI GATC 6 cut(s) 111, 189, 552, 669, 722, 833
DpnII GATC 6 cut(s) 109, 187, 550, 667, 720, 831
Eam1104I CTCTTC 3 cut(s) 10, 429, 765
EarI CTCTTC 3 cut(s) 10, 429, 765
Eco47I GGWCC 1 cut(s) 763
Eco88I CYCGRG 1 cut(s) 911
EcoRII CCWGG 1 cut(s) 765
EcoT22I ATGCAT 2 cut(s) 43, 604
FaeI CATG 3 cut(s) 221, 502, 812
FaiI YATR 9 cut(s) 219, 269, 485, 500, 562, 564, 597, 604, 810
FatI CATG 3 cut(s) 217, 498, 808
FauNDI CATATG 1 cut(s) 562
Fnu4HI GCNGC 3 cut(s) 64, 171, 369
FokI GGATG 4 cut(s) 28, 118, 337, 584
Fsp4HI GCNGC 3 cut(s) 64, 171, 369
FspBI CTAG 4 cut(s) 191, 342, 464, 503
GluI GCNGC 3 cut(s) 64, 171, 369
GsaI CCCAGC 1 cut(s) 571
HapII CCGG 1 cut(s) 26
Hin1II CATG 3 cut(s) 221, 502, 812
HindIII AAGCTT 1 cut(s) 687
HinfI GANTC 4 cut(s) 125, 401, 524, 827
HpaII CCGG 1 cut(s) 26
HphI GGTGA 3 cut(s) 236, 248, 760
Hpy166II GTNNAC 3 cut(s) 22, 291, 458
Hpy188I TCNGA 6 cut(s) 7, 105, 124, 205, 585, 727
Hpy188III TCNNGA 4 cut(s) 191, 464, 671, 911
Hpy8I GTNNAC 3 cut(s) 22, 291, 458
HpyAV CCTTC 6 cut(s) 5, 266, 703, 707, 745, 811
HpyCH4III ACNGT 2 cut(s) 683, 842
HpyCH4V TGCA 8 cut(s) 22, 33, 41, 63, 368, 470, 602, 926
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 347
HpyF3I CTNAG 2 cut(s) 174, 724
Hsp92II CATG 3 cut(s) 221, 502, 812
Kzo9I GATC 6 cut(s) 109, 187, 550, 667, 720, 831
LmnI GCTCC 1 cut(s) 718
Lsp1109I GCAGC 3 cut(s) 75, 157, 380
LweI GCATC 3 cut(s) 50, 457, 589
MaeI CTAG 4 cut(s) 191, 342, 464, 503
MalI GATC 6 cut(s) 111, 189, 552, 669, 722, 833
MboI GATC 6 cut(s) 109, 187, 550, 667, 720, 831
MflI RGATCY 4 cut(s) 187, 550, 667, 720
MhlI GDGCHC 1 cut(s) 24
MluCI AATT 5 cut(s) 161, 317, 450, 735, 785
MlyI GAGTC 2 cut(s) 533, 821
Mph1103I ATGCAT 2 cut(s) 43, 604
MseI TTAA 3 cut(s) 320, 579, 855
MslI CAYNNNNRTG 1 cut(s) 222
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 1 cut(s) 26
MspR9I CCNGG 2 cut(s) 26, 767
Mva1269I GAATGC 2 cut(s) 43, 65
MvaI CCWGG 1 cut(s) 767
MwoI GCNNNNNNNGC 2 cut(s) 72, 347
NciI CCSGG 1 cut(s) 26
NdeI CATATG 1 cut(s) 562
NdeII GATC 6 cut(s) 109, 187, 550, 667, 720, 831
NlaIII CATG 3 cut(s) 221, 502, 812
NlaIV GGNNCC 4 cut(s) 390, 552, 714, 806
NsiI ATGCAT 2 cut(s) 43, 604
NspI RCATGY 2 cut(s) 221, 502
PaeR7I CTCGAG 1 cut(s) 911
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PctI GAATGC 2 cut(s) 43, 65
PfeI GAWTC 2 cut(s) 125, 401
PkrI GCNGC 3 cut(s) 65, 172, 370
PleI GAGTC 2 cut(s) 532, 821
PpsI GAGTC 2 cut(s) 532, 821
Psp6I CCWGG 1 cut(s) 765
PspFI CCCAGC 1 cut(s) 567
PspGI CCWGG 1 cut(s) 765
PspN4I GGNNCC 4 cut(s) 390, 552, 714, 806
PspPI GGNCC 1 cut(s) 763
PstNI CAGNNNCTG 2 cut(s) 128, 209
PsuI RGATCY 4 cut(s) 187, 550, 667, 720
PvuII CAGCTG 1 cut(s) 371
RsaI GTAC 2 cut(s) 132, 533
RsaNI GTAC 2 cut(s) 131, 532
RseI CAYNNNNRTG 1 cut(s) 222
SaqAI TTAA 3 cut(s) 320, 579, 855
SatI GCNGC 3 cut(s) 64, 171, 369
Sau3AI GATC 6 cut(s) 109, 187, 550, 667, 720, 831
Sau96I GGNCC 1 cut(s) 763
SchI GAGTC 2 cut(s) 533, 821
ScrFI CCNGG 2 cut(s) 26, 767
SduI GDGCHC 1 cut(s) 24
SetI ASST 6 cut(s) 103, 373, 394, 691, 806, 877
SfaNI GCATC 3 cut(s) 50, 457, 589
Sfr274I CTCGAG 1 cut(s) 911
SinI GGWCC 1 cut(s) 763
SlaI CTCGAG 1 cut(s) 911
SmiMI CAYNNNNRTG 1 cut(s) 222
SmlI CTYRAG 1 cut(s) 911
SmoI CTYRAG 1 cut(s) 911
Sse9I AATT 5 cut(s) 161, 317, 450, 735, 785
SspI AATATT 1 cut(s) 625
SspMI CTAG 4 cut(s) 191, 342, 464, 503
StyD4I CCNGG 2 cut(s) 24, 765
TaaI ACNGT 2 cut(s) 683, 842
TaqI TCGA 2 cut(s) 830, 912
TasI AATT 5 cut(s) 161, 317, 450, 735, 785
TatI WGTACW 2 cut(s) 130, 531
TfiI GAWTC 2 cut(s) 125, 401
Tru1I TTAA 3 cut(s) 320, 579, 855
Tru9I TTAA 3 cut(s) 320, 579, 855
TseI GCWGC 3 cut(s) 63, 170, 368
TspDTI ATGAA 6 cut(s) 24, 228, 341, 347, 500, 671
VneI GTGCAC 1 cut(s) 20
VpaK11BI GGWCC 1 cut(s) 763
XapI RAATTY 1 cut(s) 785
XbaI TCTAGA 2 cut(s) 190, 463
XceI RCATGY 2 cut(s) 221, 502
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
XhoI CTCGAG 1 cut(s) 911
XspI CTAG 4 cut(s) 191, 342, 464, 503
Zsp2I ATGCAT 2 cut(s) 43, 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.