Rroxscaffold_4G00326720

Pre-mRNA-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002812
Physical Location & Seq
Forward (+)
58874495 .. 58877233
2739 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326720.1

Sequence Viewer

Length: 456 bp
ATGAAGGAGCTTGAGCTCTCCATGGTGACATCTCGGTGCTCGGCGGTTCCCATTTTGAGCGAGATCGCCGGACTCAAGCTCCTCCGCACAAAGCTATCGGCCTGTACAATCTCGGGACCGGCCCCGAACAATGCACACAAAAAACGAACAAACAAGATCGAAGTTGACATGGAAGAATACAATAAGATGAAACAGGAAGGAGCTTTTAGCTGCCTCGAAAATGGAAAGACTCCGAAGGTCTCGGAGGAGAAAGTTGACAAGATGTTGCAGGAGCTCAAGGAACTCGAAGAGAAGAAAAAGAAATTTAGCAGGAGGAGGAGGTTCCATGAGGACAAGGATATTGACTCGATCAATGACCGCAATGAGCATTTCAACAAGAAGATTGAGAGAGCCTTTGGTAAGTACACATTGGAAATCAAAAACAATCTCGAGCGAGGTACTGCATTGCCTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
SYF2

Protein Analysis

151

Amino Acids

17.45

Weight (kDa)

9.18

Isoelectric Point (pI)

62.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SYF2 PF08231 42 - 144 2.1e-30 SYF2 splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 44, 85, 358
AcsI RAATTY 1 cut(s) 302
AfaI GTAC 3 cut(s) 106, 404, 439
AgsI TTSAA 1 cut(s) 373
AluBI AGCT 7 cut(s) 10, 16, 79, 94, 203, 210, 274
AluI AGCT 7 cut(s) 10, 16, 79, 94, 203, 210, 274
Alw21I GWGCWC 3 cut(s) 18, 41, 276
Alw26I GTCTC 1 cut(s) 244
Ama87I CYCGRG 2 cut(s) 112, 428
AoxI GGCC 2 cut(s) 99, 120
ApeKI GCWGC 1 cut(s) 210
ApoI RAATTY 1 cut(s) 302
AspS9I GGNCC 2 cut(s) 116, 121
AsuHPI GGTGA 1 cut(s) 37
AvaI CYCGRG 2 cut(s) 112, 428
AvaII GGWCC 1 cut(s) 116
BanII GRGCYC 2 cut(s) 18, 276
Bbv12I GWGCWC 3 cut(s) 18, 41, 276
BbvI GCAGC 1 cut(s) 197
BcoDI GTCTC 1 cut(s) 244
BisI GCNGC 1 cut(s) 211
BlsI GCNGC 1 cut(s) 212
Bme18I GGWCC 1 cut(s) 116
BmeT110I CYCGRG 2 cut(s) 112, 428
BmgT120I GGNCC 2 cut(s) 116, 121
BmiI GGNNCC 4 cut(s) 48, 117, 123, 323
BpuEI CTTGAG 3 cut(s) 32, 59, 260
BsaI GGTCTC 1 cut(s) 244
BsaJI CCNNGG 1 cut(s) 21
BsaXI ACNNNNNCTCC 2 cut(s) 63, 93
Bse118I RCCGGY 1 cut(s) 118
Bse3DI GCAATG 2 cut(s) 367, 443
BseDI CCNNGG 1 cut(s) 21
BseMI GCAATG 2 cut(s) 367, 443
BseRI GAGGAG 4 cut(s) 71, 260, 328, 331
BseXI GCAGC 1 cut(s) 197
BshFI GGCC 2 cut(s) 101, 122
BsiHKAI GWGCWC 3 cut(s) 18, 41, 276
BsiHKCI CYCGRG 2 cut(s) 112, 428
BsiSI CCGG 2 cut(s) 69, 119
BslFI GGGAC 1 cut(s) 129
BsmAI GTCTC 1 cut(s) 244
BsmFI GGGAC 1 cut(s) 129
BsnI GGCC 2 cut(s) 101, 122
Bso31I GGTCTC 1 cut(s) 244
BsoBI CYCGRG 2 cut(s) 112, 428
Bsp1286I GDGCHC 3 cut(s) 18, 41, 276
Bsp1407I TGTACA 1 cut(s) 104
Bsp143I GATC 3 cut(s) 63, 156, 348
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 3 cut(s) 44, 85, 358
BspANI GGCC 2 cut(s) 101, 122
BspLI GGNNCC 4 cut(s) 48, 117, 123, 323
BspTNI GGTCTC 1 cut(s) 244
BsrDI GCAATG 2 cut(s) 367, 443
BsrFI RCCGGY 1 cut(s) 118
BsrGI TGTACA 1 cut(s) 104
BssAI RCCGGY 1 cut(s) 118
BssECI CCNNGG 1 cut(s) 21
BssMI GATC 3 cut(s) 63, 156, 348
BssT1I CCWWGG 1 cut(s) 21
Bst6I CTCTTC 1 cut(s) 282
BstAUI TGTACA 1 cut(s) 104
BstDSI CCRYGG 1 cut(s) 21
BstKTI GATC 3 cut(s) 66, 159, 351
BstMAI GTCTC 1 cut(s) 244
BstMBI GATC 3 cut(s) 63, 156, 348
BstV1I GCAGC 1 cut(s) 197
BsuRI GGCC 2 cut(s) 101, 122
BtgI CCRYGG 1 cut(s) 21
Cfr10I RCCGGY 1 cut(s) 118
Cfr13I GGNCC 2 cut(s) 116, 121
Csp6I GTAC 3 cut(s) 105, 403, 438
CviAII CATG 3 cut(s) 22, 169, 326
CviQI GTAC 3 cut(s) 105, 403, 438
DpnI GATC 3 cut(s) 65, 158, 350
DpnII GATC 3 cut(s) 63, 156, 348
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
Ecl136II GAGCTC 2 cut(s) 16, 274
Eco130I CCWWGG 1 cut(s) 21
Eco24I GRGCYC 2 cut(s) 18, 276
Eco31I GGTCTC 1 cut(s) 244
Eco47I GGWCC 1 cut(s) 116
Eco53kI GAGCTC 2 cut(s) 16, 274
Eco88I CYCGRG 2 cut(s) 112, 428
EcoICRI GAGCTC 2 cut(s) 16, 274
EcoT14I CCWWGG 1 cut(s) 21
EcoT38I GRGCYC 2 cut(s) 18, 276
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 3 cut(s) 25, 172, 329
FaiI YATR 3 cut(s) 23, 170, 327
FaqI GGGAC 1 cut(s) 129
FatI CATG 3 cut(s) 21, 168, 325
Fnu4HI GCNGC 1 cut(s) 211
FriOI GRGCYC 2 cut(s) 18, 276
Fsp4HI GCNGC 1 cut(s) 211
GluI GCNGC 1 cut(s) 211
HaeIII GGCC 2 cut(s) 101, 122
HapII CCGG 2 cut(s) 69, 119
Hin1II CATG 3 cut(s) 25, 172, 329
HincII GTYRAC 2 cut(s) 166, 256
HindII GTYRAC 2 cut(s) 166, 256
HinfI GANTC 3 cut(s) 72, 229, 344
HpaII CCGG 2 cut(s) 69, 119
HphI GGTGA 1 cut(s) 37
Hpy166II GTNNAC 3 cut(s) 166, 256, 405
Hpy188I TCNGA 2 cut(s) 234, 244
Hpy188III TCNNGA 2 cut(s) 114, 428
Hpy8I GTNNAC 3 cut(s) 166, 256, 405
HpyAV CCTTC 2 cut(s) 191, 229
HpyCH4V TGCA 3 cut(s) 134, 268, 443
Hsp92II CATG 3 cut(s) 25, 172, 329
Kzo9I GATC 3 cut(s) 63, 156, 348
LmnI GCTCC 4 cut(s) 7, 84, 200, 271
LpnPI CCDG 6 cut(s) 82, 115, 132, 179, 254, 295
Lsp1109I GCAGC 1 cut(s) 197
MaeIII GTNAC 1 cut(s) 25
MalI GATC 3 cut(s) 65, 158, 350
MboI GATC 3 cut(s) 63, 156, 348
MboII GAAGA 4 cut(s) 185, 299, 304, 391
MhlI GDGCHC 3 cut(s) 18, 41, 276
MluCI AATT 1 cut(s) 302
MlyI GAGTC 3 cut(s) 66, 223, 338
MnlI CCTC 8 cut(s) 92, 224, 238, 306, 309, 312, 322, 428
MseI TTAA 1 cut(s) 454
MslI CAYNNNNRTG 1 cut(s) 34
MspI CCGG 2 cut(s) 69, 119
NcoI CCATGG 1 cut(s) 21
NdeII GATC 3 cut(s) 63, 156, 348
NlaIII CATG 3 cut(s) 25, 172, 329
NlaIV GGNNCC 4 cut(s) 48, 117, 123, 323
NmeAIII GCCGAG 1 cut(s) 20
NmuCI GTSAC 1 cut(s) 25
PaeR7I CTCGAG 1 cut(s) 428
PkrI GCNGC 1 cut(s) 212
PleI GAGTC 3 cut(s) 66, 223, 338
PpsI GAGTC 3 cut(s) 66, 223, 338
Psp124BI GAGCTC 2 cut(s) 18, 276
PspN4I GGNNCC 4 cut(s) 48, 117, 123, 323
PspPI GGNCC 2 cut(s) 116, 121
RsaI GTAC 3 cut(s) 106, 404, 439
RsaNI GTAC 3 cut(s) 105, 403, 438
RseI CAYNNNNRTG 1 cut(s) 34
SacI GAGCTC 2 cut(s) 18, 276
SaqAI TTAA 1 cut(s) 454
SatI GCNGC 1 cut(s) 211
Sau3AI GATC 3 cut(s) 63, 156, 348
Sau96I GGNCC 2 cut(s) 116, 121
SchI GAGTC 3 cut(s) 66, 223, 338
SduI GDGCHC 3 cut(s) 18, 41, 276
Sfr274I CTCGAG 1 cut(s) 428
SinI GGWCC 1 cut(s) 116
SlaI CTCGAG 1 cut(s) 428
SmiMI CAYNNNNRTG 1 cut(s) 34
SmlI CTYRAG 4 cut(s) 11, 74, 275, 428
SmoI CTYRAG 4 cut(s) 11, 74, 275, 428
Sse9I AATT 1 cut(s) 302
SsiI CCGC 3 cut(s) 44, 85, 358
SstI GAGCTC 2 cut(s) 18, 276
StyI CCWWGG 1 cut(s) 21
TaqI TCGA 5 cut(s) 159, 216, 285, 347, 429
TasI AATT 1 cut(s) 302
TatI WGTACW 2 cut(s) 104, 402
Tru1I TTAA 1 cut(s) 454
Tru9I TTAA 1 cut(s) 454
TseFI GTSAC 1 cut(s) 25
TseI GCWGC 1 cut(s) 210
Tsp45I GTSAC 1 cut(s) 25
TspDTI ATGAA 2 cut(s) 17, 203
VpaK11BI GGWCC 1 cut(s) 116
XapI RAATTY 1 cut(s) 302
XhoI CTCGAG 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.