Rroxscaffold_7G00190370

Pre-mRNA-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002812
Physical Location & Seq
Forward (+)
30472739 .. 30475922
3184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00190370.1

Sequence Viewer

Length: 933 bp
ATGTCTGATGAAGGAAGAGTGCACCGGGATTGCAGGAATGCATCCAATCCCTACCACGAATGCAGCGAGTATTGCTTCAAAGTTATTGCTGAAACCAAAGCTCGGATGGATCAAAATCAATCAGAATCTGTACAAGTTAGTGGTGGTAGTGGCAAATCAAAATTAGAGGCTGCTCAGCAAGAGGAAGATCTAGATAGACCATCAGAGACTGATGAACATTCTGGTGATGGTGATGAGCCTCCTGCTGAGGAGGAGGAGAATATGGAAGGAGACTTCACAAAGTTCACAGGGAGGAAGAAAAAGTTGTGGGAATTAAGGATGAAGATGAATAAGGCTAGGAAAGCAAATCAAACAGATATTGCAGCTGAAAAGAAAAGAATGGAACCTCCACAAGAATCAAGGGGCATTTCTAAACAAAAATGGCTTGAAGAGAGGAAAAAAAGAATTGGTAAACTTCTAGATGCAAATGGTTTGGATATGAAAACCGCTTACATGCTAGATACACAAGAGATGGCAGAGTCAAAGTACAAGAAATGGGAAAAGGATCCTGCTCCATATGGCTGGGATGTTTTTAATCAGAAAACATTGTATGATGCATACAAAAAGCGAACAAAGAATATTGATATTGATTTGGAAGATTACAATAAGATGAAAGAAGAAGATCCAGAGTTCTACCGTGAAGCTTCCAGCCTTCAATACGGGAAGGCTCCAAAGATCTCAGAGGATAAAATTGACAAGATGGTGAAGGAACTAAAGGACCAGGAAGAGAAGAAAAAGAAATTCAGCAGGAGGAGGAGGTTCCATGATGAGAAGGATATTGACTCGATCAATGACCGTAATGAGCATTTTAACAAGAAGATTGAGCGAGCTTTTGGTAAATACACGCTGGAAATCAAAAACAATCTCGAGCGAGGAACTGCATTGCCTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
SYF2

Protein Analysis

310

Amino Acids

36.46

Weight (kDa)

6.33

Isoelectric Point (pI)

57.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SYF2 PF08231 156 - 303 1.5e-37 SYF2 splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 486
AclWI GGATC 4 cut(s) 117, 539, 552, 656
AcsI RAATTY 1 cut(s) 779
AfaI GTAC 2 cut(s) 132, 527
AfiI CCNNNNNNNGG 1 cut(s) 102
AgsI TTSAA 3 cut(s) 79, 428, 695
AjnI CCWGG 1 cut(s) 759
AluBI AGCT 4 cut(s) 101, 365, 683, 869
AluI AGCT 4 cut(s) 101, 365, 683, 869
Alw21I GWGCWC 1 cut(s) 24
Alw26I GTCTC 2 cut(s) 200, 264
Alw44I GTGCAC 1 cut(s) 20
AlwI GGATC 4 cut(s) 117, 539, 552, 656
AlwNI CAGNNNCTG 2 cut(s) 128, 209
Ama87I CYCGRG 1 cut(s) 905
ApaLI GTGCAC 1 cut(s) 20
ApeKI GCWGC 3 cut(s) 63, 170, 362
ApoI RAATTY 1 cut(s) 779
AspS9I GGNCC 1 cut(s) 757
AsuC2I CCSGG 1 cut(s) 26
AsuHPI GGTGA 3 cut(s) 236, 242, 754
AvaI CYCGRG 1 cut(s) 905
AvaII GGWCC 1 cut(s) 757
BaeGI GKGCMC 1 cut(s) 24
BamHI GGATCC 1 cut(s) 544
Bbv12I GWGCWC 1 cut(s) 24
BbvCI CCTCAGC 1 cut(s) 246
BbvI GCAGC 3 cut(s) 75, 157, 374
BccI CCATC 5 cut(s) 100, 208, 221, 505, 733
BciT130I CCWGG 1 cut(s) 761
BcnI CCSGG 1 cut(s) 26
BcoDI GTCTC 2 cut(s) 200, 264
BfaI CTAG 4 cut(s) 191, 336, 458, 497
BglII AGATCT 2 cut(s) 187, 714
BisI GCNGC 3 cut(s) 64, 171, 363
BlpI GCTNAGC 1 cut(s) 174
BlsI GCNGC 3 cut(s) 65, 172, 364
Bme1390I CCNGG 2 cut(s) 26, 761
Bme18I GGWCC 1 cut(s) 757
BmeT110I CYCGRG 1 cut(s) 905
BmgT120I GGNCC 1 cut(s) 757
BmiI GGNNCC 4 cut(s) 384, 546, 708, 800
BmrFI CCNGG 2 cut(s) 26, 761
BmsI GCATC 3 cut(s) 50, 451, 583
Bpu10I CCTNAGC 1 cut(s) 246
Bpu1102I GCTNAGC 1 cut(s) 174
BpuMI CCSGG 1 cut(s) 26
BsaBI GATNNNNATC 1 cut(s) 114
Bsc4I CCNNNNNNNGG 1 cut(s) 102
Bse3DI GCAATG 1 cut(s) 920
Bse8I GATNNNNATC 1 cut(s) 114
BseBI CCWGG 1 cut(s) 761
BseGI GGATG 4 cut(s) 41, 111, 324, 571
BseJI GATNNNNATC 1 cut(s) 114
BseLI CCNNNNNNNGG 1 cut(s) 102
BseMI GCAATG 1 cut(s) 920
BseMII CTCAG 3 cut(s) 188, 237, 732
BseRI GAGGAG 5 cut(s) 263, 266, 269, 805, 808
BseSI GKGCMC 1 cut(s) 24
BseXI GCAGC 3 cut(s) 75, 157, 374
BseYI CCCAGC 1 cut(s) 561
BsiHKAI GWGCWC 1 cut(s) 24
BsiHKCI CYCGRG 1 cut(s) 905
BsiSI CCGG 1 cut(s) 25
BslI CCNNNNNNNGG 1 cut(s) 102
BsmAI GTCTC 2 cut(s) 200, 264
BsmI GAATGC 2 cut(s) 43, 65
BsoBI CYCGRG 1 cut(s) 905
Bsp1286I GDGCHC 1 cut(s) 24
Bsp1407I TGTACA 1 cut(s) 130
Bsp143I GATC 6 cut(s) 109, 187, 544, 661, 714, 825
Bsp1720I GCTNAGC 1 cut(s) 174
BspACI CCGC 1 cut(s) 486
BspCNI CTCAG 3 cut(s) 187, 238, 731
BspLI GGNNCC 4 cut(s) 384, 546, 708, 800
BspPI GGATC 4 cut(s) 117, 539, 552, 656
BsrDI GCAATG 1 cut(s) 920
BsrGI TGTACA 1 cut(s) 130
BssMI GATC 6 cut(s) 109, 187, 544, 661, 714, 825
Bst2UI CCWGG 1 cut(s) 761
Bst4CI ACNGT 2 cut(s) 677, 836
Bst6I CTCTTC 3 cut(s) 10, 423, 759
BstAUI TGTACA 1 cut(s) 130
BstC8I GCNNGC 1 cut(s) 867
BstDEI CTNAG 3 cut(s) 174, 246, 718
BstF5I GGATG 4 cut(s) 41, 111, 324, 571
BstKTI GATC 6 cut(s) 112, 190, 547, 664, 717, 828
BstMAI GTCTC 2 cut(s) 200, 264
BstMBI GATC 6 cut(s) 109, 187, 544, 661, 714, 825
BstMWI GCNNNNNNNGC 2 cut(s) 72, 341
BstNI CCWGG 1 cut(s) 761
BstNSI RCATGY 1 cut(s) 496
BstSCI CCNGG 2 cut(s) 24, 759
BstSLI GKGCMC 1 cut(s) 24
BstV1I GCAGC 3 cut(s) 75, 157, 374
BstX2I RGATCY 4 cut(s) 187, 544, 661, 714
BstXI CCANNNNNNTGG 1 cut(s) 561
BstYI RGATCY 4 cut(s) 187, 544, 661, 714
BtsCI GGATG 4 cut(s) 41, 111, 324, 571
Cac8I GCNNGC 1 cut(s) 867
CaiI CAGNNNCTG 2 cut(s) 128, 209
Cfr13I GGNCC 1 cut(s) 757
Csp6I GTAC 2 cut(s) 131, 526
CviAII CATG 2 cut(s) 493, 803
CviQI GTAC 2 cut(s) 131, 526
DdeI CTNAG 3 cut(s) 174, 246, 718
DpnI GATC 6 cut(s) 111, 189, 546, 663, 716, 827
DpnII GATC 6 cut(s) 109, 187, 544, 661, 714, 825
Eam1104I CTCTTC 3 cut(s) 10, 423, 759
EarI CTCTTC 3 cut(s) 10, 423, 759
Eco47I GGWCC 1 cut(s) 757
Eco88I CYCGRG 1 cut(s) 905
EcoRII CCWGG 1 cut(s) 759
EcoT22I ATGCAT 2 cut(s) 43, 598
FaeI CATG 2 cut(s) 496, 806
FaiI YATR 8 cut(s) 263, 479, 494, 556, 558, 591, 598, 804
FatI CATG 2 cut(s) 492, 802
FauNDI CATATG 1 cut(s) 556
Fnu4HI GCNGC 3 cut(s) 64, 171, 363
FokI GGATG 4 cut(s) 28, 118, 331, 578
Fsp4HI GCNGC 3 cut(s) 64, 171, 363
FspBI CTAG 4 cut(s) 191, 336, 458, 497
GluI GCNGC 3 cut(s) 64, 171, 363
GsaI CCCAGC 1 cut(s) 565
HapII CCGG 1 cut(s) 25
Hin1II CATG 2 cut(s) 496, 806
HindIII AAGCTT 1 cut(s) 681
HinfI GANTC 4 cut(s) 125, 395, 518, 821
HpaII CCGG 1 cut(s) 25
HphI GGTGA 3 cut(s) 236, 242, 754
Hpy166II GTNNAC 3 cut(s) 22, 285, 452
Hpy188I TCNGA 6 cut(s) 7, 105, 124, 205, 579, 721
Hpy188III TCNNGA 4 cut(s) 191, 458, 665, 905
Hpy8I GTNNAC 3 cut(s) 22, 285, 452
HpyAV CCTTC 6 cut(s) 5, 260, 697, 701, 739, 805
HpyCH4III ACNGT 2 cut(s) 677, 836
HpyCH4V TGCA 8 cut(s) 22, 33, 41, 63, 362, 464, 596, 920
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 341
HpyF3I CTNAG 3 cut(s) 174, 246, 718
Hsp92II CATG 2 cut(s) 496, 806
Kzo9I GATC 6 cut(s) 109, 187, 544, 661, 714, 825
LmnI GCTCC 2 cut(s) 556, 712
Lsp1109I GCAGC 3 cut(s) 75, 157, 374
LweI GCATC 3 cut(s) 50, 451, 583
MaeI CTAG 4 cut(s) 191, 336, 458, 497
MalI GATC 6 cut(s) 111, 189, 546, 663, 716, 827
MboI GATC 6 cut(s) 109, 187, 544, 661, 714, 825
MflI RGATCY 4 cut(s) 187, 544, 661, 714
MhlI GDGCHC 1 cut(s) 24
MluCI AATT 5 cut(s) 161, 311, 444, 729, 779
MlyI GAGTC 2 cut(s) 527, 815
Mph1103I ATGCAT 2 cut(s) 43, 598
MseI TTAA 3 cut(s) 314, 573, 849
MslI CAYNNNNRTG 1 cut(s) 222
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 1 cut(s) 25
MspR9I CCNGG 2 cut(s) 26, 761
Mva1269I GAATGC 2 cut(s) 43, 65
MvaI CCWGG 1 cut(s) 761
MwoI GCNNNNNNNGC 2 cut(s) 72, 341
NciI CCSGG 1 cut(s) 26
NdeI CATATG 1 cut(s) 556
NdeII GATC 6 cut(s) 109, 187, 544, 661, 714, 825
NlaIII CATG 2 cut(s) 496, 806
NlaIV GGNNCC 4 cut(s) 384, 546, 708, 800
NsiI ATGCAT 2 cut(s) 43, 598
NspI RCATGY 1 cut(s) 496
PaeR7I CTCGAG 1 cut(s) 905
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PctI GAATGC 2 cut(s) 43, 65
PfeI GAWTC 2 cut(s) 125, 395
PkrI GCNGC 3 cut(s) 65, 172, 364
PleI GAGTC 2 cut(s) 526, 815
PpsI GAGTC 2 cut(s) 526, 815
Psp6I CCWGG 1 cut(s) 759
PspFI CCCAGC 1 cut(s) 561
PspGI CCWGG 1 cut(s) 759
PspN4I GGNNCC 4 cut(s) 384, 546, 708, 800
PspPI GGNCC 1 cut(s) 757
PstNI CAGNNNCTG 2 cut(s) 128, 209
PsuI RGATCY 4 cut(s) 187, 544, 661, 714
PvuII CAGCTG 1 cut(s) 365
RsaI GTAC 2 cut(s) 132, 527
RsaNI GTAC 2 cut(s) 131, 526
RseI CAYNNNNRTG 1 cut(s) 222
SaqAI TTAA 3 cut(s) 314, 573, 849
SatI GCNGC 3 cut(s) 64, 171, 363
Sau3AI GATC 6 cut(s) 109, 187, 544, 661, 714, 825
Sau96I GGNCC 1 cut(s) 757
SchI GAGTC 2 cut(s) 527, 815
ScrFI CCNGG 2 cut(s) 26, 761
SduI GDGCHC 1 cut(s) 24
SetI ASST 6 cut(s) 103, 367, 388, 685, 800, 871
SfaNI GCATC 3 cut(s) 50, 451, 583
Sfr274I CTCGAG 1 cut(s) 905
SinI GGWCC 1 cut(s) 757
SlaI CTCGAG 1 cut(s) 905
SmiMI CAYNNNNRTG 1 cut(s) 222
SmlI CTYRAG 1 cut(s) 905
SmoI CTYRAG 1 cut(s) 905
Sse9I AATT 5 cut(s) 161, 311, 444, 729, 779
SsiI CCGC 1 cut(s) 486
SspI AATATT 1 cut(s) 619
SspMI CTAG 4 cut(s) 191, 336, 458, 497
StyD4I CCNGG 2 cut(s) 24, 759
TaaI ACNGT 2 cut(s) 677, 836
TaqI TCGA 2 cut(s) 824, 906
TasI AATT 5 cut(s) 161, 311, 444, 729, 779
TatI WGTACW 2 cut(s) 130, 525
TfiI GAWTC 2 cut(s) 125, 395
Tru1I TTAA 3 cut(s) 314, 573, 849
Tru9I TTAA 3 cut(s) 314, 573, 849
TseI GCWGC 3 cut(s) 63, 170, 362
TspDTI ATGAA 6 cut(s) 24, 228, 335, 341, 494, 665
VneI GTGCAC 1 cut(s) 20
VpaK11BI GGWCC 1 cut(s) 757
XapI RAATTY 1 cut(s) 779
XbaI TCTAGA 2 cut(s) 190, 457
XceI RCATGY 1 cut(s) 496
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
XhoI CTCGAG 1 cut(s) 905
XspI CTAG 4 cut(s) 191, 336, 458, 497
Zsp2I ATGCAT 2 cut(s) 43, 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.