Rorug01G0037100

Pre-mRNA-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002812
Physical Location & Seq
Reverse (-)
6145807 .. 6149898
4092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0037100.1

Sequence Viewer

Length: 4092 bp
ATGGCAGTGGGAGAGATCTTTCTTGCGGCATTCCTTCAGTTGCTGCTGGACAGGTTAACGCCTCGGGAGTTCCTAGGCTACTTTGGAAGCTTGGGAGGAGTTCGCAAAAAGCTGCAGAAATGGAGGGGAACACTGTCTGCAATTGCAGCGGTGCTTTCCGATGCGGAGGAGAAGCAGCTGACGAGCAAGGCAGTGGAGCTGTGGCTGAATGATCTCAGACACTTGGCTTACGATATCGACGATCTACTCAACACCTTTTCTACTGAGATGTTAACACGCAAGCTGCTCAAGGTTGACCAAACTGGCACAAGCAAGGTACGAGGCTTCTTTACCAAAGTTCCTCACAAAGTTAAGTTCAATTTCAATATGAACTGCGAAGTAGACGACATTGCTAATCGCTTGCAAGACATATTTGACCGGAAAGAGAAACTCGGTTTGAAATATATTGAGCATACCTCTACTTCAACATCTTCATCGCACAGGACACCAAGTTCTTATGTGTTGGATGGACCTGTGGTTGGGAGAGATGAAGACACAAGAAAGATTGTGGAATTGTTGTCCAGAGGTGTTGATCCTTCTTCTCCAACAAAATATCAAGTTGTTGCCATTGTTGGTATGGGAGGACTGGGCAAAACAACGCTTGCGGGACAAGTCTTCAATGATGTAGCTGCAGTGGAACAGTTTGATCTCAAGATCTGGGTTTCAGTGTCCCATGACTTCGATCTTCAAACGGTGACGAGAACTATTTTTAAGGAAGTCACATCTCGGCCATGTGATATGGATGAGCTCAGTCGACTTCAAGATAATCTGAGCAAGGAGATCGATGGTAAAAGGTTTTTGATTGTTTTAGATGATGTCTGGAGCACGTGTGATTATGATTCATGGACAAAACTGCAAGCTCCCCTCCGTGGCGGAGCCAAGGGAAGTAAGGTAATGGTGACAACACGTGATGAAAAAGTAGCTGCATTGACGGGAGCCCCAGCCGCTGAAGTTTATTATTTAAAGACTCTATCAGATGAAGGTTGTTTGCAAGTATTTGAGCAGCATGTTAGCAATGACAGGCCACCGAATTTCGAGTTGCTTAAGAAGAAAATTGTTACGAACTGCAATGGATTGCCATTGGCTGCAAAAACTCTTGGTGGTGTTTTACGTTGTAAAGAAACTGACAAGTGGGAGGAAATACTGAATGACAAATTGTGGAGTATATCAGATGGGAGTAAAATACTCCCTGTACTGAGATTGAGTTATCATTATCTCCCTTCAACTTTGAAGAGGTGCTTTGCCTATTGCTCAATACTTCCCAATGACTATGAATTTGGGAAAACCCAATTGATCCTTCTGTGGATGGCAGAGGGTTTCCTTGAGCAACCAGAAGGGACTAAAGCAATGGAAGATATCGGTGACGAATATTTTGGGGAGCTATTGTCTCGGTCATTATTTCAAAACTCGGGCAAAAACAGTTTGTGCTATGTAATGCATGACCTTGTTGGTGATTTGGCACGATGGGCTGCTGGAGACACATTTTGCAGATTGGAGGATAAACTGCATGGTAGATGTTCTACAAATACTCGTCATCTAACTTACGTTTCTGGTAAGTTCGATGGGGTAAAAAGATTTGAGACATTTTCTGAAGCAAAACGCTTGCGGACTTTCCTACCACTTCCTGTTTCCGGTGGTTATGAGAATTATCTAACTCGTTATGCTACTTTTGATTTATTGCCACAATTGAAGTACTTGCGGGTGCTCTCCTTCAATGGTTATAAACTGACCGAGGTGCCAAATTCCGTAGGTAAGTTGAGACATCTACGGTACCTTGATCTTTCTCACACATTAATAACGAGTTTGCCTGAGTCAACATGCATGCTTTACAACTTACAGACGTTAATATTAGAGAATTGTTCAAAATTGAAGACCCTACCTTCAAACATGAGCAATTTATCTAATTTACGTCATCTCAAGAATTCAAACATGCCTTCATTCGAAGAAATGCCTCCCCAAGTAAGTCGGTTGACTCGTCTGCGAACTTTGCGTAATTTTGTGGTGGGGAAATGTAGTGCATCAAGGATTGGAGAGATAGGGTCATTGCATCTTCGTGGGACCTTGAGCGTTGGAAGATTGGAAAATGTGATTGATGTTGGGGATGCGAAGACGGCTCAAATAATGAACAAGGAAGGGCTGAATACCTTGCAATTGGAATGGAGTGGCACAAGTGAGAACGAGTCGGAGGTGCTGTGCAGTTTAGAACCTCATAAAAAGCTTCTACATCTCACTATCAAAGGTTACAATGGTTTCGAATTTTCAAAATGGATTGGACATCCTTCATTCTCTGATATGACATTTGTGAAGTTGGAGAATTGTAAAAATTGTCAATTCTTACCTCCTCTCGGCCAATTACCATCTCTGAAAAACCTTTGGATAGAAGGACTGGCCAGTGTTGAAAGTGTGGGTGTTGAGTTTTACGGAGAGTGTAGCTTGCCTTTTCCAGTGTTGGAAGCCCTGATTTTCAAGGATATGCAAAATTGGAAGGAGTGGCTTCCTTGCAAAAGAGATGAAGAAATTCAAGTTTTGCCATGCATGGAAAAGCTTTTGGTCCTTAGTTGCCCCAAATTGGAAGGTTGGTTACCCAAGATCATGGATTCATTATCAGATCTGCGAATACGTGAATGTGAAGAGTTAGTGGTTTCAATAGCCAATTATAAACATCTTCGTAAGCTGTTCATCGACGGTTGTAAAAGGGTGGTGCACAGAAGTGGAGTTAAGTTTGAGTCACTGGAGGATATGGGGCTTTATAATATTTCAGAGTTCAGACTCGAAATAGACGGGTTCATCAGAGGATTGCCAAAGCTTCAAAATTTGTGGATTACTGGTTGTGAAGAGTTGACATGTTTATGGGAGAATGAGGATATATGGCTGCAGCGTGGGATTTCTAATATTATTGGAGGCAACATGTCTGACTCCGCCTCTCACTTTATTCAAGAGCTAAGAGCACTCAAGCAACTTGTGTTAACTGGATGCTCAAATCTAATTTCTTTACCAGAAGCTAGTTGGCCACCTTGTCTTGAATATGTAAAGATGGAGTCGTGTAATTCTTTGACATATTTCGTAAGGTATCAAGTACCGTCAAGCATAAGAAGAATAGAGATAGCAAAATGTCCAAATTTGAAACTATTAGTGAAACTACTAGAGGGTTGTCTGGAGCACTTGAAGATAACAGAGTGTGCATCTTTGACATCCTTATCAGGCAAAGGAGGCCGACTACCCAGAACGCTCAAATATCTTTATTTACGTGACTGTCAACAATTGGAGTCAATAAGCAAAACATTCCACGATGACACTTGTCTTGGAGATATTAGGATAAAGAGGTGTGCAAATCTCAAATCCTTACCAGAGGGGCTATGCCATCTCTCCGCTCTTCGCATTTTATATATAGATGAATGTGGAAGTCTCGTTTCCTTCCCAAGAGGAGGGTTGCCATCCAACCTGATAGACTTTCGTATCGACAATTGTGATCAATTGGAAGCCCTCCCCAGAGGCATGGACAACCTCAGCTCTCTTCAGACTTTATTGTTGGAATACTGTGGAGGTAATTTGGCATCCATTATAGAAGAGGGTTTCCCACCCAACCTAATTAATCTTCATATTTGGACTCCCAAAAGCTGCAAGCCTCTCTCAGAGTGGGGGCTTCACCACTTGGACAGACTCACCTCTCTTAAAGGCTTGTGGATCGGTGGTGTAGATCCAGATCTGGTGTCCTTTCCGCCAAAGGAGGTGCTGCTCCCTAAATCTCTCATTCAACTCGAGATTGGAGAGTTCCCAAATCTGAAGCGCCTGTCATCATCTTTTCAATCACTCACCTCTCTTGAATCCCTTTCAATTGGTGGATGCCCAAAGCTAGCATCTATTATTCCAGAAAAAGAGGATCATCTGCCTCTTTCACTTACACAGCTTCGCATCCGTGAGGGCTGTCCGCTGCTCACAAAGAAATACCAACCAGGTAAAGGACGACACTGGCCCAAAGAAATAGCCCACATCCCTTACGTTTATGTTGGAGACTTCAAGGACGAAGCCGACGCCGACCGCCGCTGGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
SYF2

Protein Analysis

1363

Amino Acids

153.95

Weight (kDa)

6.98

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 100 1.5e-20 Rx N-terminal domain
NB-ARC PF00931 179 - 352 8.2e-37 NB-ARC domain
WHD_DRP PF23559 432 - 500 5.4e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 573 - 839 2e-24 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 690 - 808 2.2e-32 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1761, 2697, 2790
Acc65I GGTACC 1 cut(s) 1809
AccB1I GGYRCC 2 cut(s) 1774, 1809
AccBSI CCGCTC 1 cut(s) 3410
AccI GTMKAC 2 cut(s) 381, 793
AclWI GGATC 5 cut(s) 566, 1327, 3731, 3731, 3927
AcoI YGGCCR 4 cut(s) 767, 2386, 2427, 3047
AcsI RAATTY 8 cut(s) 1069, 1313, 1780, 1960, 2294, 2556, 2851, 3157
AcuI CTGAAG 5 cut(s) 20, 1008, 1650, 3539, 3842
AcvI CACGTG 2 cut(s) 867, 947
AcyI GRCGYC 1 cut(s) 4071
AfaI GTAC 5 cut(s) 318, 1233, 1733, 1811, 3117
AfiI CCNNNNNNNGG 8 cut(s) 518, 908, 1670, 2384, 2608, 3464, 3998, 4084
AflII CTTAAG 1 cut(s) 1082
AflIII ACRYGT 4 cut(s) 866, 944, 2882, 2946
AjnI CCWGG 1 cut(s) 3991
AjuI GAANNNNNNNTTGG 2 cut(s) 1320, 1352
AleI CACNNNNGTG 1 cut(s) 2748
AloI GAACNNNNNNTCC 2 cut(s) 475, 507
Alw21I GWGCWC 6 cut(s) 789, 866, 1746, 2745, 2989, 3201
Alw26I GTCTC 6 cut(s) 1431, 1509, 1613, 1792, 3449, 4044
Alw44I GTGCAC 1 cut(s) 2741
AlwI GGATC 5 cut(s) 566, 1327, 3731, 3731, 3927
AlwNI CAGNNNCTG 2 cut(s) 43, 986
Ama87I CYCGRG 3 cut(s) 63, 1447, 3797
AoxI GGCC 7 cut(s) 767, 1061, 2386, 2427, 3047, 3250, 4010
ApaLI GTGCAC 1 cut(s) 2741
ApoI RAATTY 8 cut(s) 1069, 1313, 1780, 1960, 2294, 2556, 2851, 3157
ArsI GACNNNNNNTTYG 4 cut(s) 1298, 1330, 1394, 1426
AseI ATTAAT 2 cut(s) 1832, 3630
Asp700I GAANNNNTTC 1 cut(s) 2556
Asp718I GGTACC 1 cut(s) 1809
AspA2I CCTAGG 1 cut(s) 73
AspLEI GCGC 1 cut(s) 3828
AspS9I GGNCC 4 cut(s) 509, 2097, 2590, 4011
AsuHPI GGTGA 7 cut(s) 745, 949, 1412, 1502, 3677, 3694, 3844
AsuII TTCGAA 2 cut(s) 1980, 2292
AsuNHI GCTAGC 1 cut(s) 3892
AvaI CYCGRG 3 cut(s) 63, 1447, 3797
AvaII GGWCC 3 cut(s) 509, 2097, 2590
AvrII CCTAGG 1 cut(s) 73
BaeGI GKGCMC 1 cut(s) 2745
BaeI ACNNNNGTAYC 2 cut(s) 3478, 3511
BalI TGGCCA 2 cut(s) 2429, 3049
BanI GGYRCC 2 cut(s) 1774, 1809
BanII GRGCYC 2 cut(s) 789, 979
BarI GAAGNNNNNNTAC 2 cut(s) 2604, 2636
BbrPI CACGTG 2 cut(s) 867, 947
BbsI GAAGAC 4 cut(s) 537, 646, 1916, 2153
Bbv12I GWGCWC 6 cut(s) 789, 866, 1746, 2745, 2989, 3201
BbvCI CCTCAGC 1 cut(s) 3545
BceAI ACGGC 1 cut(s) 2166
BcgI CGANNNNNNTGC 2 cut(s) 802, 836
BciT130I CCWGG 1 cut(s) 3993
BclI TGATCA 1 cut(s) 3508
BcoDI GTCTC 6 cut(s) 1431, 1509, 1613, 1792, 3449, 4044
BfaI CTAG 4 cut(s) 74, 3042, 3182, 3893
BfmI CTRYAG 3 cut(s) 113, 669, 2912
BfoI RGCGCY 1 cut(s) 3829
BfrI CTTAAG 1 cut(s) 1082
BglII AGATCT 4 cut(s) 15, 693, 2647, 3742
BlnI CCTAGG 1 cut(s) 73
BmcAI AGTACT 1 cut(s) 1733
Bme1390I CCNGG 1 cut(s) 3993
Bme18I GGWCC 3 cut(s) 509, 2097, 2590
BmeT110I CYCGRG 3 cut(s) 63, 1447, 3797
BmgT120I GGNCC 4 cut(s) 509, 2097, 2590, 4011
BmiI GGNNCC 5 cut(s) 916, 976, 1776, 1811, 2098
BmrFI CCNGG 1 cut(s) 3993
BmrI ACTGGG 1 cut(s) 635
BmtI GCTAGC 1 cut(s) 3896
BmuI ACTGGG 1 cut(s) 635
BoxI GACNNNNGTC 1 cut(s) 3336
BpiI GAAGAC 4 cut(s) 537, 646, 1916, 2153
BplI GAGNNNNNCTC 2 cut(s) 440, 472
BpmI CTGGAG 4 cut(s) 880, 1533, 2792, 3215
Bpu10I CCTNAGC 1 cut(s) 3545
Bpu14I TTCGAA 2 cut(s) 1980, 2292
BpuEI CTTGAG 6 cut(s) 272, 674, 1382, 1940, 2122, 2975
Bsa29I ATCGAT 1 cut(s) 822
BsaAI YACGTR 4 cut(s) 867, 947, 2660, 3287
BsaBI GATNNNNATC 1 cut(s) 3741
BsaHI GRCGYC 1 cut(s) 4071
BsaJI CCNNGG 5 cut(s) 62, 73, 907, 918, 1770
BsaWI WCCGGW 2 cut(s) 417, 1670
BsaXI ACNNNNNCTCC 6 cut(s) 853, 883, 1409, 1439, 2216, 2246
Bsc4I CCNNNNNNNGG 8 cut(s) 518, 908, 1670, 2384, 2608, 3464, 3998, 4084
Bse1I ACTGG 9 cut(s) 307, 630, 2430, 2430, 2483, 2775, 2869, 3013, 4013
Bse3DI GCAATG 5 cut(s) 387, 1060, 1114, 1392, 2081
Bse8I GATNNNNATC 1 cut(s) 3741
BseBI CCWGG 1 cut(s) 3993
BseCI ATCGAT 1 cut(s) 822
BseDI CCNNGG 5 cut(s) 62, 73, 907, 918, 1770
BseJI GATNNNNATC 1 cut(s) 3741
BseLI CCNNNNNNNGG 8 cut(s) 518, 908, 1670, 2384, 2608, 3464, 3998, 4084
BseMI GCAATG 5 cut(s) 387, 1060, 1114, 1392, 2081
BseMII CTCAG 8 cut(s) 229, 255, 800, 802, 1226, 1839, 3559, 3684
BseNI ACTGG 9 cut(s) 307, 630, 2430, 2430, 2483, 2775, 2869, 3013, 4013
BseRI GAGGAG 4 cut(s) 111, 182, 2370, 3477
BseSI GKGCMC 1 cut(s) 2745
BseYI CCCAGC 2 cut(s) 979, 4083
BsgI GTGCAG 1 cut(s) 2254
Bsh1285I CGRYCG 1 cut(s) 4078
BshFI GGCC 7 cut(s) 769, 1063, 2388, 2429, 3049, 3252, 4012
BshNI GGYRCC 2 cut(s) 1774, 1809
BshVI ATCGAT 1 cut(s) 822
BsiEI CGRYCG 1 cut(s) 4078
BsiHKAI GWGCWC 6 cut(s) 789, 866, 1746, 2745, 2989, 3201
BsiHKCI CYCGRG 3 cut(s) 63, 1447, 3797
BsiSI CCGG 2 cut(s) 418, 1671
BslFI GGGAC 4 cut(s) 660, 694, 1390, 2110
BslI CCNNNNNNNGG 8 cut(s) 518, 908, 1670, 2384, 2608, 3464, 3998, 4084
BsmAI GTCTC 6 cut(s) 1431, 1509, 1613, 1792, 3449, 4044
BsmFI GGGAC 4 cut(s) 660, 694, 1390, 2110
BsmI GAATGC 1 cut(s) 29
BsnI GGCC 7 cut(s) 769, 1063, 2388, 2429, 3049, 3252, 4012
BsoBI CYCGRG 3 cut(s) 63, 1447, 3797
Bsp119I TTCGAA 2 cut(s) 1980, 2292
Bsp1286I GDGCHC 7 cut(s) 789, 866, 979, 1746, 2745, 2989, 3201
BspANI GGCC 7 cut(s) 769, 1063, 2388, 2429, 3049, 3252, 4012
BspCNI CTCAG 8 cut(s) 228, 256, 801, 801, 1227, 1840, 3558, 3683
BspDI ATCGAT 1 cut(s) 822
BspLI GGNNCC 5 cut(s) 916, 976, 1776, 1811, 2098
BspMAI CTGCAG 3 cut(s) 117, 673, 2916
BspOI GCTAGC 1 cut(s) 3896
BspPI GGATC 5 cut(s) 566, 1327, 3731, 3731, 3927
BspQI GCTCTTC 1 cut(s) 3417
BspT104I TTCGAA 2 cut(s) 1980, 2292
BspT107I GGYRCC 2 cut(s) 1774, 1809
BspTI CTTAAG 1 cut(s) 1082
BsrBI CCGCTC 1 cut(s) 3410
BsrDI GCAATG 5 cut(s) 387, 1060, 1114, 1392, 2081
BsrI ACTGG 9 cut(s) 307, 630, 2430, 2430, 2483, 2775, 2869, 3013, 4013
BssECI CCNNGG 5 cut(s) 62, 73, 907, 918, 1770
BssNI GRCGYC 1 cut(s) 4071
BssT1I CCWWGG 2 cut(s) 73, 918
Bst2UI CCWGG 1 cut(s) 3993
Bst4CI ACNGT 9 cut(s) 135, 681, 733, 1460, 1809, 2726, 3120, 3293, 3578
Bst6I CTCTTC 6 cut(s) 1265, 2664, 2868, 3417, 3558, 3600
BstACI GRCGYC 1 cut(s) 4071
BstAFI CTTAAG 1 cut(s) 1082
BstBAI YACGTR 4 cut(s) 867, 947, 2660, 3287
BstBI TTCGAA 2 cut(s) 1980, 2292
BstC8I GCNNGC 9 cut(s) 281, 401, 642, 897, 1643, 1862, 2474, 3662, 3894
BstDSI CCRYGG 1 cut(s) 907
BstEII GGTNACC 1 cut(s) 2619
BstH2I RGCGCY 1 cut(s) 3829
BstHHI GCGC 1 cut(s) 3828
BstMAI GTCTC 6 cut(s) 1431, 1509, 1613, 1792, 3449, 4044
BstMCI CGRYCG 1 cut(s) 4078
BstMWI GCNNNNNNNGC 6 cut(s) 146, 983, 1505, 2026, 2150, 3249
BstNI CCWGG 1 cut(s) 3993
BstNSI RCATGY 6 cut(s) 1049, 1860, 1864, 1972, 2886, 2950
BstPAI GACNNNNGTC 1 cut(s) 3336
BstPI GGTNACC 1 cut(s) 2619
BstSCI CCNGG 1 cut(s) 3991
BstSFI CTRYAG 3 cut(s) 113, 669, 2912
BstSLI GKGCMC 1 cut(s) 2745
BstV2I GAAGAC 4 cut(s) 537, 646, 1916, 2153
BstX2I RGATCY 5 cut(s) 15, 693, 2647, 3736, 3742
BstXI CCANNNNNNTGG 2 cut(s) 2632, 3535
BstYI RGATCY 5 cut(s) 15, 693, 2647, 3736, 3742
Bsu15I ATCGAT 1 cut(s) 822
BsuRI GGCC 7 cut(s) 769, 1063, 2388, 2429, 3049, 3252, 4012
BsuTUI ATCGAT 1 cut(s) 822
BtgI CCRYGG 1 cut(s) 907
BtgZI GCGATG 1 cut(s) 459
BtsI GCAGTG 3 cut(s) 12, 198, 678
BtsIMutI CAGTG 9 cut(s) 12, 131, 198, 678, 711, 2437, 2490, 2768, 4006
Cac8I GCNNGC 9 cut(s) 281, 401, 642, 897, 1643, 1862, 2474, 3662, 3894
CaiI CAGNNNCTG 2 cut(s) 43, 986
CfoI GCGC 1 cut(s) 3828
Cfr13I GGNCC 4 cut(s) 509, 2097, 2590, 4011
ClaI ATCGAT 1 cut(s) 822
CseI GACGC 1 cut(s) 4079
CsiI ACCWGGT 1 cut(s) 3991
Csp6I GTAC 5 cut(s) 317, 1232, 1732, 1810, 3116
CspCI CAANNNNNGTGG 2 cut(s) 2837, 2872
CviQI GTAC 5 cut(s) 317, 1232, 1732, 1810, 3116
DraI TTTAAA 1 cut(s) 1002
EaeI YGGCCR 4 cut(s) 767, 2386, 2427, 3047
Eam1104I CTCTTC 6 cut(s) 1265, 2664, 2868, 3417, 3558, 3600
EarI CTCTTC 6 cut(s) 1265, 2664, 2868, 3417, 3558, 3600
EciI GGCGGA 3 cut(s) 927, 2947, 3747
Ecl136II GAGCTC 1 cut(s) 787
Eco130I CCWWGG 2 cut(s) 73, 918
Eco24I GRGCYC 2 cut(s) 789, 979
Eco32I GATATC 2 cut(s) 235, 1396
Eco47I GGWCC 3 cut(s) 509, 2097, 2590
Eco53kI GAGCTC 1 cut(s) 787
Eco57I CTGAAG 5 cut(s) 20, 1008, 1650, 3539, 3842
Eco72I CACGTG 2 cut(s) 867, 947
Eco88I CYCGRG 3 cut(s) 63, 1447, 3797
Eco91I GGTNACC 1 cut(s) 2619
EcoICRI GAGCTC 1 cut(s) 787
EcoO109I RGGNCCY 1 cut(s) 2097
EcoO65I GGTNACC 1 cut(s) 2619
EcoRI GAATTC 1 cut(s) 1960
EcoRII CCWGG 1 cut(s) 3991
EcoRV GATATC 2 cut(s) 235, 1396
EcoT14I CCWWGG 2 cut(s) 73, 918
EcoT22I ATGCAT 3 cut(s) 1479, 1862, 2576
EcoT38I GRGCYC 2 cut(s) 789, 979
ErhI CCWWGG 2 cut(s) 73, 918
FalI AAGNNNNNCTT 2 cut(s) 1262, 1294
FaqI GGGAC 4 cut(s) 660, 694, 1390, 2110
FauI CCCGC 2 cut(s) 637, 1731
FbaI TGATCA 1 cut(s) 3508
FblI GTMKAC 2 cut(s) 381, 793
FriOI GRGCYC 2 cut(s) 789, 979
FspBI CTAG 4 cut(s) 74, 3042, 3182, 3893
GlaI GCGC 1 cut(s) 3827
GsaI CCCAGC 2 cut(s) 983, 4087
GsuI CTGGAG 4 cut(s) 880, 1533, 2792, 3215
HaeII RGCGCY 1 cut(s) 3829
HaeIII GGCC 7 cut(s) 769, 1063, 2388, 2429, 3049, 3252, 4012
HapII CCGG 2 cut(s) 418, 1671
HgaI GACGC 1 cut(s) 4079
HhaI GCGC 1 cut(s) 3828
Hin1I GRCGYC 1 cut(s) 4071
Hin6I GCGC 1 cut(s) 3826
HinP1I GCGC 1 cut(s) 3826
HincII GTYRAC 9 cut(s) 57, 273, 295, 794, 1854, 2010, 2880, 3006, 3296
HindII GTYRAC 9 cut(s) 57, 273, 295, 794, 1854, 2010, 2880, 3006, 3296
HindIII AAGCTT 4 cut(s) 88, 2255, 2582, 2843
HpaI GTTAAC 3 cut(s) 57, 273, 3006
HpaII CCGG 2 cut(s) 418, 1671
HphI GGTGA 7 cut(s) 745, 949, 1412, 1502, 3677, 3694, 3844
Hpy99I CGWCG 3 cut(s) 242, 2726, 4073
HpyCH4III ACNGT 9 cut(s) 135, 681, 733, 1460, 1809, 2726, 3120, 3293, 3578
HpyCH4IV ACGT 9 cut(s) 866, 946, 1150, 1584, 1880, 1948, 2659, 3286, 4038
HpyF10VI GCNNNNNNNGC 6 cut(s) 146, 983, 1505, 2026, 2150, 3249
HpySE526I ACGT 9 cut(s) 866, 946, 1150, 1584, 1880, 1948, 2659, 3286, 4038
Hsp92I GRCGYC 1 cut(s) 4071
HspAI GCGC 1 cut(s) 3826
KpnI GGTACC 1 cut(s) 1813
Ksp22I TGATCA 1 cut(s) 3508
KspAI GTTAAC 3 cut(s) 57, 273, 3006
LguI GCTCTTC 1 cut(s) 3417
LmnI GCTCC 8 cut(s) 196, 861, 904, 914, 974, 1417, 3196, 3780
MabI ACCWGGT 1 cut(s) 3991
MaeI CTAG 4 cut(s) 74, 3042, 3182, 3893
MaeII ACGT 9 cut(s) 866, 946, 1150, 1584, 1880, 1948, 2659, 3286, 4038
MaeIII GTNAC 9 cut(s) 733, 757, 937, 1096, 1400, 2279, 2619, 2766, 3287
MbiI CCGCTC 1 cut(s) 3410
MfeI CAATTG 8 cut(s) 141, 1328, 1724, 2189, 3299, 3502, 3512, 3873
MflI RGATCY 5 cut(s) 15, 693, 2647, 3736, 3742
MhlI GDGCHC 7 cut(s) 789, 866, 979, 1746, 2745, 2989, 3201
MlsI TGGCCA 2 cut(s) 2429, 3049
MluNI TGGCCA 2 cut(s) 2429, 3049
MmeI TCCRAC 9 cut(s) 483, 608, 2089, 2202, 2328, 2469, 3501, 3549, 4027
Mox20I TGGCCA 2 cut(s) 2429, 3049
Mph1103I ATGCAT 3 cut(s) 1479, 1862, 2576
MroXI GAANNNNTTC 1 cut(s) 2556
MscI TGGCCA 2 cut(s) 2429, 3049
MslI CAYNNNNRTG 3 cut(s) 2091, 2748, 2887
Msp20I TGGCCA 2 cut(s) 2429, 3049
MspA1I CMGCKG 5 cut(s) 149, 178, 986, 3970, 4083
MspCI CTTAAG 1 cut(s) 1082
MspI CCGG 2 cut(s) 418, 1671
MspR9I CCNGG 1 cut(s) 3993
MunI CAATTG 8 cut(s) 141, 1328, 1724, 2189, 3299, 3502, 3512, 3873
Mva1269I GAATGC 1 cut(s) 29
MvaI CCWGG 1 cut(s) 3993
MwoI GCNNNNNNNGC 6 cut(s) 146, 983, 1505, 2026, 2150, 3249
NheI GCTAGC 1 cut(s) 3892
NlaIV GGNNCC 5 cut(s) 916, 976, 1776, 1811, 2098
NmeAIII GCCGAG 2 cut(s) 745, 2364
NmuCI GTSAC 6 cut(s) 733, 757, 937, 1400, 2766, 3287
NsiI ATGCAT 3 cut(s) 1479, 1862, 2576
NspI RCATGY 6 cut(s) 1049, 1860, 1864, 1972, 2886, 2950
NspV TTCGAA 2 cut(s) 1980, 2292
OliI CACNNNNGTG 1 cut(s) 2748
PaeI GCATGC 1 cut(s) 1864
PaeR7I CTCGAG 1 cut(s) 3797
PciI ACATGT 2 cut(s) 2882, 2946
PciSI GCTCTTC 1 cut(s) 3417
PcsI WCGNNNNNNNCGW 2 cut(s) 237, 2011
PctI GAATGC 1 cut(s) 29
PdmI GAANNNNTTC 1 cut(s) 2556
PfeI GAWTC 3 cut(s) 878, 2636, 3863
PmaCI CACGTG 2 cut(s) 867, 947
PmlI CACGTG 2 cut(s) 867, 947
Ppu21I YACGTR 4 cut(s) 867, 947, 2660, 3287
PpuMI RGGWCCY 1 cut(s) 2097
PscI ACATGT 2 cut(s) 2882, 2946
PshAI GACNNNNGTC 1 cut(s) 3336
PshBI ATTAAT 2 cut(s) 1832, 3630
PsiI TTATAA 3 cut(s) 1761, 2697, 2790
Psp124BI GAGCTC 1 cut(s) 789
Psp5II RGGWCCY 1 cut(s) 2097
Psp6I CCWGG 1 cut(s) 3991
PspCI CACGTG 2 cut(s) 867, 947
PspEI GGTNACC 1 cut(s) 2619
PspFI CCCAGC 2 cut(s) 979, 4083
PspGI CCWGG 1 cut(s) 3991
PspN4I GGNNCC 5 cut(s) 916, 976, 1776, 1811, 2098
PspPI GGNCC 4 cut(s) 509, 2097, 2590, 4011
PspPPI RGGWCCY 1 cut(s) 2097
PstI CTGCAG 3 cut(s) 117, 673, 2916
PstNI CAGNNNCTG 2 cut(s) 43, 986
PsuI RGATCY 5 cut(s) 15, 693, 2647, 3736, 3742
PvuII CAGCTG 1 cut(s) 178
RsaI GTAC 5 cut(s) 318, 1233, 1733, 1811, 3117
RsaNI GTAC 5 cut(s) 317, 1232, 1732, 1810, 3116
RseI CAYNNNNRTG 3 cut(s) 2091, 2748, 2887
SacI GAGCTC 1 cut(s) 789
SalI GTCGAC 1 cut(s) 792
SapI GCTCTTC 1 cut(s) 3417
Sau96I GGNCC 4 cut(s) 509, 2097, 2590, 4011
ScaI AGTACT 1 cut(s) 1733
ScrFI CCNGG 1 cut(s) 3993
SduI GDGCHC 7 cut(s) 789, 866, 979, 1746, 2745, 2989, 3201
SexAI ACCWGGT 1 cut(s) 3991
SfcI CTRYAG 3 cut(s) 113, 669, 2912
Sfr274I CTCGAG 1 cut(s) 3797
SfuI TTCGAA 2 cut(s) 1980, 2292
SinI GGWCC 3 cut(s) 509, 2097, 2590
SlaI CTCGAG 1 cut(s) 3797
SmiMI CAYNNNNRTG 3 cut(s) 2091, 2748, 2887
SmlI CTYRAG 8 cut(s) 287, 689, 1082, 1361, 1955, 2101, 2990, 3797
SmoI CTYRAG 8 cut(s) 287, 689, 1082, 1361, 1955, 2101, 2990, 3797
SphI GCATGC 1 cut(s) 1864
SspI AATATT 4 cut(s) 1409, 1887, 2794, 2932
SspMI CTAG 4 cut(s) 74, 3042, 3182, 3893
SstI GAGCTC 1 cut(s) 789
StyD4I CCNGG 1 cut(s) 3991
StyI CCWWGG 2 cut(s) 73, 918
TaaI ACNGT 9 cut(s) 135, 681, 733, 1460, 1809, 2726, 3120, 3293, 3578
TaiI ACGT 9 cut(s) 869, 949, 1153, 1587, 1883, 1951, 2662, 3289, 4041
TaqII GACCGA 2 cut(s) 1419, 1784
TatI WGTACW 2 cut(s) 1231, 1731
TauI GCSGC 3 cut(s) 29, 986, 4083
TfiI GAWTC 3 cut(s) 878, 2636, 3863
TscAI CASTG 9 cut(s) 12, 138, 198, 678, 711, 2437, 2490, 2775, 4013
TseFI GTSAC 6 cut(s) 733, 757, 937, 1400, 2766, 3287
Tsp45I GTSAC 6 cut(s) 733, 757, 937, 1400, 2766, 3287
TspGWI ACGGA 4 cut(s) 896, 1774, 2475, 3944
TspRI CASTG 9 cut(s) 12, 138, 198, 678, 711, 2437, 2490, 2775, 4013
Vha464I CTTAAG 1 cut(s) 1082
VneI GTGCAC 1 cut(s) 2741
VpaK11BI GGWCC 3 cut(s) 509, 2097, 2590
VspI ATTAAT 2 cut(s) 1832, 3630
XapI RAATTY 8 cut(s) 1069, 1313, 1780, 1960, 2294, 2556, 2851, 3157
XceI RCATGY 6 cut(s) 1049, 1860, 1864, 1972, 2886, 2950
XcmI CCANNNNNNNNNTGG 2 cut(s) 613, 3042
XhoI CTCGAG 1 cut(s) 3797
XmaJI CCTAGG 1 cut(s) 73
XmiI GTMKAC 2 cut(s) 381, 793
XmnI GAANNNNTTC 1 cut(s) 2556
XspI CTAG 4 cut(s) 74, 3042, 3182, 3893
ZrmI AGTACT 1 cut(s) 1733
Zsp2I ATGCAT 3 cut(s) 1479, 1862, 2576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.