MD05G1122700.v1.1

Pre-mRNA-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002812
Physical Location & Seq
Reverse (-)
24544908 .. 24547532
2625 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1122700.v1.1.491

Sequence Viewer

Length: 879 bp
ATGTCTGATGAGCAAAGAGTTCACCCGGATTGCAGAAATGCATCCAATCCATACCATGAATGCAGCGAGTATTGCTTTACAGTTATCGCCGAAGCCAAGGCTCGAATGCCTCAAATCCAATCAGATCCTGAAGAACTTGCTGATGATGGCGATGCCTATCCTCAAGAACATGCTGATGATGATGATGCCCATCCTCTCGAGGAGAATATGGAAGGAGACTTCCAAAACTTAACAGGGAGAAAGAGAAAATTGGCGGAATTGAGGAAGAAATTGGGTGAGGCAAAGAAACAAAATCAAACGGAGATTACGGCCGAAAAGAAAAGACTGGAACCCCGACAAGAATCCAGGGGTATTTCGAAACAAAAATGGGTTGAAGAGAGGAAGAAAAGAATTGGCAAACTTCTAGATGCCAATGGTTTGGATATGAAAAGTGCATACATGCTAGATACGCAAGAGATGGCAGAGGAAAAATATAAGAAATGGGAAAAGGATCCTGCCCCAGCTGGCTGGGATGTTTTCAATCAGAAAACTCTGTACAATGCATACAAAAAGCGGACAAAGAACATTGATATTGACTTGGAAGAATACAATAGGATGAAAGAAGCTGATCCGGAGTTCTATCGTGAAGCTTCCAGCCTCCAATATGGGAAGGCTCCAAAGATATCAGAGGATAAACTTGACAAGATGGTGAAGGAACTCAAGGATCGGGAAGAGAAGAAGGCTTCCTTCAGCAGGAGGAGGAGGTTCCACGATGAGAAAGATATTGACTCGATCAATGACCGCAATGAGCATTTCAACAAGAAGATTGAGCGAGCCTTTGGTAAATACACGTTGGAAATCAAAAACAATCTTGAGCGCGGAACTGCATTGCCCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
SYF2

Protein Analysis

293

Amino Acids

34.39

Weight (kDa)

6.02

Isoelectric Point (pI)

50.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SYF2 PF08231 138 - 285 1.1e-37 SYF2 splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 858
AccIII TCCGGA 1 cut(s) 610
AciI CCGC 4 cut(s) 254, 553, 781, 858
AclWI GGATC 5 cut(s) 119, 485, 498, 602, 711
AcoI YGGCCR 1 cut(s) 309
AcuI CTGAAG 2 cut(s) 150, 712
AfaI GTAC 1 cut(s) 536
AfiI CCNNNNNNNGG 1 cut(s) 732
AflIII ACRYGT 1 cut(s) 828
AgsI TTSAA 3 cut(s) 374, 520, 796
AjnI CCWGG 1 cut(s) 344
AluBI AGCT 3 cut(s) 503, 605, 629
AluI AGCT 3 cut(s) 503, 605, 629
Alw26I GTCTC 1 cut(s) 210
AlwI GGATC 5 cut(s) 119, 485, 498, 602, 711
AlwNI CAGNNNCTG 1 cut(s) 128
Ama87I CYCGRG 1 cut(s) 197
Aor13HI TCCGGA 1 cut(s) 610
AoxI GGCC 1 cut(s) 309
ApeKI GCWGC 1 cut(s) 63
AspLEI GCGC 1 cut(s) 858
AsuC2I CCSGG 1 cut(s) 26
AsuHPI GGTGA 3 cut(s) 14, 287, 700
AsuII TTCGAA 1 cut(s) 356
AvaI CYCGRG 1 cut(s) 197
BamHI GGATCC 1 cut(s) 490
BbvI GCAGC 1 cut(s) 75
BccI CCATC 4 cut(s) 140, 198, 451, 679
BceAI ACGGC 1 cut(s) 324
BciT130I CCWGG 1 cut(s) 346
BcnI CCSGG 1 cut(s) 26
BcoDI GTCTC 1 cut(s) 210
BfaI CTAG 2 cut(s) 404, 443
BisI GCNGC 1 cut(s) 64
BlsI GCNGC 1 cut(s) 65
Bme1390I CCNGG 2 cut(s) 26, 346
BmeT110I CYCGRG 1 cut(s) 197
BmiI GGNNCC 4 cut(s) 330, 492, 654, 746
BmrFI CCNGG 2 cut(s) 26, 346
BmsI GCATC 4 cut(s) 50, 142, 175, 397
Bpu14I TTCGAA 1 cut(s) 356
BpuEI CTTGAG 3 cut(s) 147, 683, 872
BpuMI CCSGG 1 cut(s) 26
BsaBI GATNNNNATC 2 cut(s) 156, 189
BsaJI CCNNGG 2 cut(s) 96, 345
BsaWI WCCGGW 1 cut(s) 610
Bsc4I CCNNNNNNNGG 1 cut(s) 732
Bse1I ACTGG 1 cut(s) 330
Bse3DI GCAATG 2 cut(s) 790, 866
Bse8I GATNNNNATC 2 cut(s) 156, 189
BseAI TCCGGA 1 cut(s) 610
BseBI CCWGG 1 cut(s) 346
BseDI CCNNGG 2 cut(s) 96, 345
BseGI GGATG 4 cut(s) 41, 190, 517, 600
BseJI GATNNNNATC 2 cut(s) 156, 189
BseLI CCNNNNNNNGG 1 cut(s) 732
BseMI GCAATG 2 cut(s) 790, 866
BseNI ACTGG 1 cut(s) 330
BseRI GAGGAG 3 cut(s) 215, 751, 754
BseX3I CGGCCG 1 cut(s) 309
BseXI GCAGC 1 cut(s) 75
BseYI CCCAGC 2 cut(s) 499, 507
Bsh1236I CGCG 1 cut(s) 858
Bsh1285I CGRYCG 1 cut(s) 312
BshFI GGCC 1 cut(s) 311
BsiEI CGRYCG 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 197
BsiSI CCGG 2 cut(s) 26, 611
BslI CCNNNNNNNGG 1 cut(s) 732
BsmAI GTCTC 1 cut(s) 210
BsmI GAATGC 2 cut(s) 65, 111
BsnI GGCC 1 cut(s) 311
BsoBI CYCGRG 1 cut(s) 197
Bsp119I TTCGAA 1 cut(s) 356
Bsp13I TCCGGA 1 cut(s) 610
Bsp1407I TGTACA 1 cut(s) 534
Bsp143I GATC 5 cut(s) 124, 490, 607, 703, 771
BspACI CCGC 4 cut(s) 254, 553, 781, 858
BspANI GGCC 1 cut(s) 311
BspEI TCCGGA 1 cut(s) 610
BspFNI CGCG 1 cut(s) 858
BspLI GGNNCC 4 cut(s) 330, 492, 654, 746
BspPI GGATC 5 cut(s) 119, 485, 498, 602, 711
BspT104I TTCGAA 1 cut(s) 356
BsrDI GCAATG 2 cut(s) 790, 866
BsrGI TGTACA 1 cut(s) 534
BsrI ACTGG 1 cut(s) 330
BssECI CCNNGG 2 cut(s) 96, 345
BssMI GATC 5 cut(s) 124, 490, 607, 703, 771
BssT1I CCWWGG 1 cut(s) 96
Bst2UI CCWGG 1 cut(s) 346
Bst4CI ACNGT 1 cut(s) 82
Bst6I CTCTTC 2 cut(s) 369, 705
BstAUI TGTACA 1 cut(s) 534
BstBI TTCGAA 1 cut(s) 356
BstC8I GCNNGC 2 cut(s) 505, 813
BstENI CCTNNNNNAGG 1 cut(s) 730
BstF5I GGATG 4 cut(s) 41, 190, 517, 600
BstFNI CGCG 1 cut(s) 858
BstHHI GCGC 1 cut(s) 858
BstKTI GATC 5 cut(s) 127, 493, 610, 706, 774
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 5 cut(s) 124, 490, 607, 703, 771
BstMCI CGRYCG 1 cut(s) 312
BstMWI GCNNNNNNNGC 2 cut(s) 72, 448
BstNI CCWGG 1 cut(s) 346
BstNSI RCATGY 2 cut(s) 173, 442
BstSCI CCNGG 2 cut(s) 24, 344
BstUI CGCG 1 cut(s) 858
BstV1I GCAGC 1 cut(s) 75
BstX2I RGATCY 2 cut(s) 124, 490
BstXI CCANNNNNNTGG 2 cut(s) 418, 507
BstYI RGATCY 2 cut(s) 124, 490
BstZI CGGCCG 1 cut(s) 309
BsuRI GGCC 1 cut(s) 311
BtgZI GCGATG 1 cut(s) 165
BtsCI GGATG 4 cut(s) 41, 190, 517, 600
Cac8I GCNNGC 2 cut(s) 505, 813
CaiI CAGNNNCTG 1 cut(s) 128
CfoI GCGC 1 cut(s) 858
Csp6I GTAC 1 cut(s) 535
CviAII CATG 3 cut(s) 56, 170, 439
CviQI GTAC 1 cut(s) 535
DpnI GATC 5 cut(s) 126, 492, 609, 705, 773
DpnII GATC 5 cut(s) 124, 490, 607, 703, 771
EaeI YGGCCR 1 cut(s) 309
EagI CGGCCG 1 cut(s) 309
Eam1104I CTCTTC 2 cut(s) 369, 705
EarI CTCTTC 2 cut(s) 369, 705
EciI GGCGGA 1 cut(s) 269
EclXI CGGCCG 1 cut(s) 309
Eco130I CCWWGG 1 cut(s) 96
Eco32I GATATC 1 cut(s) 663
Eco52I CGGCCG 1 cut(s) 309
Eco57I CTGAAG 2 cut(s) 150, 712
Eco88I CYCGRG 1 cut(s) 197
EcoNI CCTNNNNNAGG 1 cut(s) 730
EcoRII CCWGG 1 cut(s) 344
EcoRV GATATC 1 cut(s) 663
EcoT14I CCWWGG 1 cut(s) 96
EcoT22I ATGCAT 2 cut(s) 43, 544
ErhI CCWWGG 1 cut(s) 96
FaeI CATG 3 cut(s) 59, 173, 442
FalI AAGNNNNNCTT 2 cut(s) 710, 742
FatI CATG 3 cut(s) 55, 169, 438
Fnu4HI GCNGC 1 cut(s) 64
FokI GGATG 4 cut(s) 28, 177, 524, 607
Fsp4HI GCNGC 1 cut(s) 64
FspBI CTAG 2 cut(s) 404, 443
GlaI GCGC 1 cut(s) 857
GluI GCNGC 1 cut(s) 64
GsaI CCCAGC 2 cut(s) 503, 511
HaeIII GGCC 1 cut(s) 311
HapII CCGG 2 cut(s) 26, 611
HhaI GCGC 1 cut(s) 858
Hin1II CATG 3 cut(s) 59, 173, 442
Hin6I GCGC 1 cut(s) 856
HinP1I GCGC 1 cut(s) 856
HindIII AAGCTT 1 cut(s) 627
HinfI GANTC 2 cut(s) 341, 767
HpaII CCGG 2 cut(s) 26, 611
HphI GGTGA 3 cut(s) 14, 287, 700
Hpy166II GTNNAC 1 cut(s) 22
Hpy188I TCNGA 4 cut(s) 7, 124, 525, 667
Hpy188III TCNNGA 8 cut(s) 128, 164, 197, 404, 611, 623, 707, 851
Hpy8I GTNNAC 1 cut(s) 22
HpyAV CCTTC 5 cut(s) 206, 643, 685, 712, 736
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4IV ACGT 1 cut(s) 830
HpyCH4V TGCA 6 cut(s) 33, 41, 63, 434, 542, 866
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 448
HpySE526I ACGT 1 cut(s) 830
Hsp92II CATG 3 cut(s) 59, 173, 442
HspAI GCGC 1 cut(s) 856
Kpn2I TCCGGA 1 cut(s) 610
Kzo9I GATC 5 cut(s) 124, 490, 607, 703, 771
LmnI GCTCC 1 cut(s) 658
Lsp1109I GCAGC 1 cut(s) 75
LweI GCATC 4 cut(s) 50, 142, 175, 397
MaeI CTAG 2 cut(s) 404, 443
MaeII ACGT 1 cut(s) 830
MalI GATC 5 cut(s) 126, 492, 609, 705, 773
MboI GATC 5 cut(s) 124, 490, 607, 703, 771
MboII GAAGA 8 cut(s) 143, 277, 386, 394, 593, 722, 727, 814
MflI RGATCY 2 cut(s) 124, 490
MluCI AATT 4 cut(s) 248, 257, 269, 390
MlyI GAGTC 1 cut(s) 761
MmeI TCCRAC 1 cut(s) 813
Mph1103I ATGCAT 2 cut(s) 43, 544
MroI TCCGGA 1 cut(s) 610
MseI TTAA 1 cut(s) 230
MslI CAYNNNNRTG 1 cut(s) 174
MspA1I CMGCKG 1 cut(s) 503
MspI CCGG 2 cut(s) 26, 611
MspR9I CCNGG 2 cut(s) 26, 346
Mva1269I GAATGC 2 cut(s) 65, 111
MvaI CCWGG 1 cut(s) 346
MvnI CGCG 1 cut(s) 858
MwoI GCNNNNNNNGC 2 cut(s) 72, 448
NciI CCSGG 1 cut(s) 26
NdeII GATC 5 cut(s) 124, 490, 607, 703, 771
NlaIII CATG 3 cut(s) 59, 173, 442
NlaIV GGNNCC 4 cut(s) 330, 492, 654, 746
NsiI ATGCAT 2 cut(s) 43, 544
NspI RCATGY 2 cut(s) 173, 442
NspV TTCGAA 1 cut(s) 356
PaeR7I CTCGAG 1 cut(s) 197
PctI GAATGC 2 cut(s) 65, 111
PfeI GAWTC 1 cut(s) 341
PkrI GCNGC 1 cut(s) 65
PleI GAGTC 1 cut(s) 761
PpsI GAGTC 1 cut(s) 761
Psp6I CCWGG 1 cut(s) 344
PspFI CCCAGC 2 cut(s) 499, 507
PspGI CCWGG 1 cut(s) 344
PspN4I GGNNCC 4 cut(s) 330, 492, 654, 746
PstNI CAGNNNCTG 1 cut(s) 128
PsuI RGATCY 2 cut(s) 124, 490
PvuII CAGCTG 1 cut(s) 503
RsaI GTAC 1 cut(s) 536
RsaNI GTAC 1 cut(s) 535
RseI CAYNNNNRTG 1 cut(s) 174
SaqAI TTAA 1 cut(s) 230
SatI GCNGC 1 cut(s) 64
Sau3AI GATC 5 cut(s) 124, 490, 607, 703, 771
SchI GAGTC 1 cut(s) 761
ScrFI CCNGG 2 cut(s) 26, 346
SetI ASST 5 cut(s) 505, 607, 631, 746, 833
SfaNI GCATC 4 cut(s) 50, 142, 175, 397
Sfr274I CTCGAG 1 cut(s) 197
SfuI TTCGAA 1 cut(s) 356
SlaI CTCGAG 1 cut(s) 197
SmiMI CAYNNNNRTG 1 cut(s) 174
SmlI CTYRAG 4 cut(s) 162, 197, 698, 851
SmoI CTYRAG 4 cut(s) 162, 197, 698, 851
Sse9I AATT 4 cut(s) 248, 257, 269, 390
SsiI CCGC 4 cut(s) 254, 553, 781, 858
SspMI CTAG 2 cut(s) 404, 443
StyD4I CCNGG 2 cut(s) 24, 344
StyI CCWWGG 1 cut(s) 96
TaaI ACNGT 1 cut(s) 82
TaiI ACGT 1 cut(s) 833
TaqI TCGA 4 cut(s) 103, 198, 356, 770
TasI AATT 4 cut(s) 248, 257, 269, 390
TatI WGTACW 1 cut(s) 534
TfiI GAWTC 1 cut(s) 341
Tru1I TTAA 1 cut(s) 230
Tru9I TTAA 1 cut(s) 230
TseI GCWGC 1 cut(s) 63
TspDTI ATGAA 3 cut(s) 72, 440, 611
TspGWI ACGGA 1 cut(s) 314
XagI CCTNNNNNAGG 1 cut(s) 730
XbaI TCTAGA 1 cut(s) 403
XceI RCATGY 2 cut(s) 173, 442
XhoI CTCGAG 1 cut(s) 197
XspI CTAG 2 cut(s) 404, 443
Zsp2I ATGCAT 2 cut(s) 43, 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.