RchiOBHm_Chr1g0323251

Pre-mRNA-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002812
Physical Location & Seq
Reverse (-)
10800709 .. 10802873
2165 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55317

Sequence Viewer

Length: 873 bp
ATGATGTCTTCTAGTGAAAGAACAGTGCACTCGGAGTGCAAGAATTCGTCCAATCTCGACCATGAATGCCGTGAATATTGCTTCAAAGCTATTTTAGAAGCCAGAGATGGGATGGATCTAAATGATGTAAGTCAACTGCTGGATTCTGTACAAGATAATAGTGGCAACTCAAACTTAGAGGCTGCTCCTCAGCGACAGAAAGAAGATGCTGATAATGGTGTTGACCACACAAAGTTGAAAGGGAGGAAGAAAAAATTGAAGGAATTGCGGAAGAAGGTGAAAGAGGCTATAGATAAGAATCAAACACAGGTTAAAGCCGAACAGAAAAGAATGGAGCCTCCAGAAGAACCGTCGAGGGGGATTTCGAAACAAAAATGGCTTGAAGAGAGGGAGAGAAAGATGGGGAAACTCTTGGATGCAAATGGTTTGGATTTGAAAAGAGCATACATGCTAGATACACAAGAGATGGCCGAGGCAAAATACAAGAGATGGAAAAAGAAACCTGCACCATGTGGCTGGGATGTTTTTAATTCGAAAACATTGTACAATGCACACAAAAAACGAACAAACAAGATCGAAGTTGACATGGAAGAATACAATAAGATGAAACAGGAAGGAGCTTTTAGCTGCCTCGAAAATGGAAAGACTCCGAAGGTCTCGGAGGAGAAAGTTGACAAGATGTTGCAGGAGCTCAAGGAACTCGAAGAGAATAAAAAGAAATTTAGCAGGAGGAGGAGGTTCCATGAGGACAAGGATATTGACTCGATCAATGACCGCAATGAGCATTTCAACAAGAAGATTGAGAGAGCCTTTGGTAAGTACACATTGGAAATCAAAAACAATCTTGAGCGAGGTACTGCATTGCCTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
SYF2

Protein Analysis

290

Amino Acids

33.99

Weight (kDa)

9.07

Isoelectric Point (pI)

55.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SYF2 PF08231 141 - 283 8.7e-34 SYF2 splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 511
AciI CCGC 2 cut(s) 268, 775
AclWI GGATC 1 cut(s) 123
AcoI YGGCCR 1 cut(s) 468
AcsI RAATTY 2 cut(s) 43, 719
AdeI CACNNNGTG 1 cut(s) 512
AfaI GTAC 4 cut(s) 150, 545, 821, 856
AfiI CCNNNNNNNGG 2 cut(s) 108, 356
AgsI TTSAA 6 cut(s) 85, 238, 259, 383, 436, 790
AluBI AGCT 4 cut(s) 89, 620, 627, 691
AluI AGCT 4 cut(s) 89, 620, 627, 691
Alw21I GWGCWC 2 cut(s) 30, 693
Alw26I GTCTC 1 cut(s) 661
Alw44I GTGCAC 1 cut(s) 26
AlwI GGATC 1 cut(s) 123
AoxI GGCC 1 cut(s) 468
ApaLI GTGCAC 1 cut(s) 26
ApeKI GCWGC 2 cut(s) 182, 627
ApoI RAATTY 2 cut(s) 43, 719
AsuHPI GGTGA 1 cut(s) 289
AsuII TTCGAA 2 cut(s) 365, 533
BaeGI GKGCMC 1 cut(s) 30
BanII GRGCYC 1 cut(s) 693
Bbv12I GWGCWC 2 cut(s) 30, 693
BbvCI CCTCAGC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 169, 614
BccI CCATC 5 cut(s) 101, 106, 394, 460, 483
BceAI ACGGC 1 cut(s) 54
BcoDI GTCTC 1 cut(s) 661
BfaI CTAG 2 cut(s) 12, 452
BfmI CTRYAG 1 cut(s) 288
BfuAI ACCTGC 1 cut(s) 511
BisI GCNGC 2 cut(s) 183, 628
BlsI GCNGC 2 cut(s) 184, 629
BmiI GGNNCC 2 cut(s) 336, 740
BmsI GCATC 2 cut(s) 196, 406
BpmI CTGGAG 1 cut(s) 324
Bpu10I CCTNAGC 1 cut(s) 189
Bpu14I TTCGAA 2 cut(s) 365, 533
BpuEI CTTGAG 2 cut(s) 677, 866
BsaBI GATNNNNATC 1 cut(s) 297
BsaI GGTCTC 1 cut(s) 661
BsaJI CCNNGG 1 cut(s) 471
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 356
Bse3DI GCAATG 2 cut(s) 784, 860
Bse8I GATNNNNATC 1 cut(s) 297
BseDI CCNNGG 1 cut(s) 471
BseGI GGATG 3 cut(s) 117, 421, 526
BseJI GATNNNNATC 1 cut(s) 297
BseLI CCNNNNNNNGG 2 cut(s) 108, 356
BseMI GCAATG 2 cut(s) 784, 860
BseMII CTCAG 1 cut(s) 203
BseRI GAGGAG 4 cut(s) 177, 677, 745, 748
BseSI GKGCMC 1 cut(s) 30
BseXI GCAGC 2 cut(s) 169, 614
BseYI CCCAGC 1 cut(s) 516
BsgI GTGCAG 1 cut(s) 489
BshFI GGCC 1 cut(s) 470
BsiHKAI GWGCWC 2 cut(s) 30, 693
BslI CCNNNNNNNGG 2 cut(s) 108, 356
BsmAI GTCTC 1 cut(s) 661
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 1 cut(s) 470
Bso31I GGTCTC 1 cut(s) 661
Bsp119I TTCGAA 2 cut(s) 365, 533
Bsp1286I GDGCHC 2 cut(s) 30, 693
Bsp1407I TGTACA 2 cut(s) 148, 543
Bsp143I GATC 3 cut(s) 115, 573, 765
BspACI CCGC 2 cut(s) 268, 775
BspANI GGCC 1 cut(s) 470
BspCNI CTCAG 1 cut(s) 202
BspLI GGNNCC 2 cut(s) 336, 740
BspMI ACCTGC 1 cut(s) 511
BspPI GGATC 1 cut(s) 123
BspT104I TTCGAA 2 cut(s) 365, 533
BspTNI GGTCTC 1 cut(s) 661
BsrDI GCAATG 2 cut(s) 784, 860
BsrGI TGTACA 2 cut(s) 148, 543
BssECI CCNNGG 1 cut(s) 471
BssMI GATC 3 cut(s) 115, 573, 765
Bst4CI ACNGT 2 cut(s) 25, 351
Bst6I CTCTTC 2 cut(s) 378, 699
BstAUI TGTACA 2 cut(s) 148, 543
BstBI TTCGAA 2 cut(s) 365, 533
BstDEI CTNAG 2 cut(s) 175, 189
BstF5I GGATG 3 cut(s) 117, 421, 526
BstKTI GATC 3 cut(s) 118, 576, 768
BstMAI GTCTC 1 cut(s) 661
BstMBI GATC 3 cut(s) 115, 573, 765
BstNSI RCATGY 1 cut(s) 451
BstSFI CTRYAG 1 cut(s) 288
BstSLI GKGCMC 1 cut(s) 30
BstV1I GCAGC 2 cut(s) 169, 614
BstX2I RGATCY 1 cut(s) 115
BstXI CCANNNNNNTGG 1 cut(s) 516
BstYI RGATCY 1 cut(s) 115
BsuRI GGCC 1 cut(s) 470
BtsCI GGATG 3 cut(s) 117, 421, 526
BtsIMutI CAGTG 1 cut(s) 30
BveI ACCTGC 1 cut(s) 511
Csp6I GTAC 4 cut(s) 149, 544, 820, 855
CviAII CATG 5 cut(s) 62, 448, 510, 586, 743
CviQI GTAC 4 cut(s) 149, 544, 820, 855
DdeI CTNAG 2 cut(s) 175, 189
DpnI GATC 3 cut(s) 117, 575, 767
DpnII GATC 3 cut(s) 115, 573, 765
DraIII CACNNNGTG 1 cut(s) 512
EaeI YGGCCR 1 cut(s) 468
Eam1104I CTCTTC 2 cut(s) 378, 699
EarI CTCTTC 2 cut(s) 378, 699
Ecl136II GAGCTC 1 cut(s) 691
Eco24I GRGCYC 1 cut(s) 693
Eco31I GGTCTC 1 cut(s) 661
Eco53kI GAGCTC 1 cut(s) 691
EcoICRI GAGCTC 1 cut(s) 691
EcoRI GAATTC 1 cut(s) 43
EcoT38I GRGCYC 1 cut(s) 693
FaeI CATG 5 cut(s) 65, 451, 513, 589, 746
FaiI YATR 7 cut(s) 63, 290, 445, 449, 511, 587, 744
FatI CATG 5 cut(s) 61, 447, 509, 585, 742
Fnu4HI GCNGC 2 cut(s) 183, 628
FokI GGATG 3 cut(s) 124, 428, 533
FriOI GRGCYC 1 cut(s) 693
Fsp4HI GCNGC 2 cut(s) 183, 628
FspBI CTAG 2 cut(s) 12, 452
GluI GCNGC 2 cut(s) 183, 628
GsaI CCCAGC 1 cut(s) 520
GsuI CTGGAG 1 cut(s) 324
HaeIII GGCC 1 cut(s) 470
Hin1II CATG 5 cut(s) 65, 451, 513, 589, 746
HincII GTYRAC 4 cut(s) 134, 223, 583, 673
HindII GTYRAC 4 cut(s) 134, 223, 583, 673
HinfI GANTC 4 cut(s) 143, 298, 646, 761
HphI GGTGA 1 cut(s) 289
Hpy166II GTNNAC 6 cut(s) 28, 134, 223, 583, 673, 822
Hpy188I TCNGA 3 cut(s) 34, 651, 661
Hpy188III TCNNGA 3 cut(s) 56, 341, 845
Hpy8I GTNNAC 6 cut(s) 28, 134, 223, 583, 673, 822
Hpy99I CGWCG 1 cut(s) 355
HpyAV CCTTC 4 cut(s) 253, 268, 608, 646
HpyCH4III ACNGT 2 cut(s) 25, 351
HpyCH4V TGCA 7 cut(s) 28, 39, 419, 506, 551, 685, 860
HpyF3I CTNAG 2 cut(s) 175, 189
Hsp92II CATG 5 cut(s) 65, 451, 513, 589, 746
Kzo9I GATC 3 cut(s) 115, 573, 765
LmnI GCTCC 4 cut(s) 190, 334, 617, 688
LpnPI CCDG 9 cut(s) 115, 125, 293, 354, 502, 516, 596, 671, 712
Lsp1109I GCAGC 2 cut(s) 169, 614
LweI GCATC 2 cut(s) 196, 406
MaeI CTAG 2 cut(s) 12, 452
MalI GATC 3 cut(s) 117, 575, 767
MboI GATC 3 cut(s) 115, 573, 765
MboII GAAGA 8 cut(s) 215, 259, 283, 356, 395, 602, 716, 808
MflI RGATCY 1 cut(s) 115
MhlI GDGCHC 2 cut(s) 30, 693
MluCI AATT 5 cut(s) 43, 254, 263, 529, 719
MlyI GAGTC 2 cut(s) 640, 755
MseI TTAA 3 cut(s) 312, 528, 871
Mva1269I GAATGC 1 cut(s) 71
NdeII GATC 3 cut(s) 115, 573, 765
NlaIII CATG 5 cut(s) 65, 451, 513, 589, 746
NlaIV GGNNCC 2 cut(s) 336, 740
NmeAIII GCCGAG 1 cut(s) 496
NspI RCATGY 1 cut(s) 451
NspV TTCGAA 2 cut(s) 365, 533
PctI GAATGC 1 cut(s) 71
PfeI GAWTC 2 cut(s) 143, 298
PkrI GCNGC 2 cut(s) 184, 629
PleI GAGTC 2 cut(s) 640, 755
PpsI GAGTC 2 cut(s) 640, 755
Psp124BI GAGCTC 1 cut(s) 693
PspFI CCCAGC 1 cut(s) 516
PspN4I GGNNCC 2 cut(s) 336, 740
PsuI RGATCY 1 cut(s) 115
RsaI GTAC 4 cut(s) 150, 545, 821, 856
RsaNI GTAC 4 cut(s) 149, 544, 820, 855
SacI GAGCTC 1 cut(s) 693
SaqAI TTAA 3 cut(s) 312, 528, 871
SatI GCNGC 2 cut(s) 183, 628
Sau3AI GATC 3 cut(s) 115, 573, 765
SchI GAGTC 2 cut(s) 640, 755
SduI GDGCHC 2 cut(s) 30, 693
SfaNI GCATC 2 cut(s) 196, 406
SfcI CTRYAG 1 cut(s) 288
SfuI TTCGAA 2 cut(s) 365, 533
SmlI CTYRAG 2 cut(s) 692, 845
SmoI CTYRAG 2 cut(s) 692, 845
Sse9I AATT 5 cut(s) 43, 254, 263, 529, 719
SsiI CCGC 2 cut(s) 268, 775
SspI AATATT 1 cut(s) 77
SspMI CTAG 2 cut(s) 12, 452
SstI GAGCTC 1 cut(s) 693
TaaI ACNGT 2 cut(s) 25, 351
TaqI TCGA 8 cut(s) 57, 353, 365, 533, 576, 633, 702, 764
TasI AATT 5 cut(s) 43, 254, 263, 529, 719
TatI WGTACW 3 cut(s) 148, 543, 819
TfiI GAWTC 2 cut(s) 143, 298
Tru1I TTAA 3 cut(s) 312, 528, 871
Tru9I TTAA 3 cut(s) 312, 528, 871
TscAI CASTG 1 cut(s) 30
TseI GCWGC 2 cut(s) 182, 627
TspDTI ATGAA 2 cut(s) 78, 620
TspRI CASTG 1 cut(s) 30
VneI GTGCAC 1 cut(s) 26
XapI RAATTY 2 cut(s) 43, 719
XceI RCATGY 1 cut(s) 451
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
XspI CTAG 2 cut(s) 12, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.