FvH4_2g15711

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004843
Physical Location & Seq
Reverse (-)
13785683 .. 13786326
644 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g15711.t1

Sequence Viewer

Length: 483 bp
ATGACAAAACAGATTTCAGTTACAAGCCATTATAAAGCGAGCAACTCATCATCATCATCTGCAGAAAGAAAGCAGAACCGGAAGGCATCGCTAAAGAAAGCGCTGTTTGCACATTTCAATCAGAAGAACAAGAGCTGCAGAAGCAAACTAGTATCGAGGGCCACTGCCATTGCAAGGGCGAAAAGCTTCAGCGGTATTGATGTATTTTCCCAGTCGACCCAGGAGGACAATGGGGCAGCAGTGAGGTTGAAGATTGTGGTCATGAAGGAGGATTTGGAGGAAGTTTTGGAACTCATGAGGAATTATAACGACAACAATAACAATCATAACACTGCTGACGATGACAATGGTGATGATGTTGAGGAAGATGAAGAAGCATTACTGTCGATGGATGAGCGCCTAATTCAGTGGCGGAAAAATCATAGGCGCCACAATTGTTGGAATCCTACTCTGCAAACCATCCCGGAAGAAATTTTAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

161

Amino Acids

18.27

Weight (kDa)

6.97

Isoelectric Point (pI)

50.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 33, 306
AccB1I GGYRCC 1 cut(s) 426
AccI GTMKAC 1 cut(s) 215
AciI CCGC 2 cut(s) 192, 412
AcsI RAATTY 1 cut(s) 471
AcuI CTGAAG 1 cut(s) 172
AcyI GRCGYC 1 cut(s) 427
AfeI AGCGCT 1 cut(s) 102
AfiI CCNNNNNNNGG 1 cut(s) 174
AgsI TTSAA 2 cut(s) 118, 250
AhlI ACTAGT 1 cut(s) 148
AjnI CCWGG 1 cut(s) 219
AjuI GAANNNNNNNTTGG 2 cut(s) 257, 289
AluBI AGCT 2 cut(s) 135, 186
AluI AGCT 2 cut(s) 135, 186
Aor51HI AGCGCT 1 cut(s) 102
AoxI GGCC 1 cut(s) 159
ApeKI GCWGC 2 cut(s) 135, 236
ApoI RAATTY 1 cut(s) 471
Asp700I GAANNNNTTC 1 cut(s) 185
AspLEI GCGC 3 cut(s) 103, 399, 429
AspS9I GGNCC 1 cut(s) 159
AsuC2I CCSGG 1 cut(s) 464
AsuHPI GGTGA 1 cut(s) 362
BanI GGYRCC 1 cut(s) 426
BarI GAAGNNNNNNTAC 2 cut(s) 363, 395
BbvI GCAGC 2 cut(s) 122, 248
BccI CCATC 2 cut(s) 382, 467
BcgI CGANNNNNNTGC 2 cut(s) 366, 400
BciT130I CCWGG 1 cut(s) 221
BcnI CCSGG 1 cut(s) 464
BcuI ACTAGT 1 cut(s) 148
BfaI CTAG 1 cut(s) 149
BfmI CTRYAG 2 cut(s) 60, 136
BfoI RGCGCY 3 cut(s) 104, 400, 430
BisI GCNGC 2 cut(s) 136, 237
BlsI GCNGC 2 cut(s) 137, 238
Bme1390I CCNGG 2 cut(s) 221, 464
BmgT120I GGNCC 1 cut(s) 159
BmiI GGNNCC 1 cut(s) 428
BmrFI CCNGG 2 cut(s) 221, 464
BmrI ACTGGG 1 cut(s) 205
BmsI GCATC 1 cut(s) 95
BmuI ACTGGG 1 cut(s) 205
BpuMI CCSGG 1 cut(s) 464
BsaHI GRCGYC 1 cut(s) 427
BsaJI CCNNGG 1 cut(s) 219
BsaWI WCCGGW 1 cut(s) 78
Bsc4I CCNNNNNNNGG 1 cut(s) 174
Bse1I ACTGG 1 cut(s) 211
Bse3DI GCAATG 1 cut(s) 168
BseBI CCWGG 1 cut(s) 221
BseDI CCNNGG 1 cut(s) 219
BseGI GGATG 2 cut(s) 397, 459
BseLI CCNNNNNNNGG 1 cut(s) 174
BseMI GCAATG 1 cut(s) 168
BseNI ACTGG 1 cut(s) 211
BseXI GCAGC 2 cut(s) 122, 248
BshFI GGCC 1 cut(s) 161
BshNI GGYRCC 1 cut(s) 426
BsiSI CCGG 2 cut(s) 79, 464
BslI CCNNNNNNNGG 1 cut(s) 174
BsnI GGCC 1 cut(s) 161
BspACI CCGC 2 cut(s) 192, 412
BspANI GGCC 1 cut(s) 161
BspHI TCATGA 2 cut(s) 261, 294
BspLI GGNNCC 1 cut(s) 428
BspMAI CTGCAG 2 cut(s) 64, 140
BspT107I GGYRCC 1 cut(s) 426
BsrDI GCAATG 1 cut(s) 168
BsrI ACTGG 1 cut(s) 211
BssECI CCNNGG 1 cut(s) 219
BssNI GRCGYC 1 cut(s) 427
Bst2UI CCWGG 1 cut(s) 221
Bst4CI ACNGT 1 cut(s) 384
BstACI GRCGYC 1 cut(s) 427
BstC8I GCNNGC 1 cut(s) 40
BstF5I GGATG 2 cut(s) 397, 459
BstH2I RGCGCY 3 cut(s) 104, 400, 430
BstHHI GCGC 3 cut(s) 103, 399, 429
BstMWI GCNNNNNNNGC 2 cut(s) 107, 141
BstNI CCWGG 1 cut(s) 221
BstSCI CCNGG 2 cut(s) 219, 462
BstSFI CTRYAG 2 cut(s) 60, 136
BstV1I GCAGC 2 cut(s) 122, 248
BsuRI GGCC 1 cut(s) 161
BtgZI GCGATG 1 cut(s) 72
BtsCI GGATG 2 cut(s) 397, 459
BtsI GCAGTG 3 cut(s) 162, 246, 330
BtsIMutI CAGTG 4 cut(s) 162, 246, 330, 413
Cac8I GCNNGC 1 cut(s) 40
CciI TCATGA 2 cut(s) 261, 294
CfoI GCGC 3 cut(s) 103, 399, 429
Cfr13I GGNCC 1 cut(s) 159
CspCI CAANNNNNGTGG 4 cut(s) 151, 186, 419, 454
CviAII CATG 2 cut(s) 262, 295
CviJI RGCY 5 cut(s) 27, 135, 161, 186, 479
CviKI_1 RGCY 5 cut(s) 27, 135, 161, 186, 479
DinI GGCGCC 1 cut(s) 428
EciI GGCGGA 1 cut(s) 427
Eco47III AGCGCT 1 cut(s) 102
Eco57I CTGAAG 1 cut(s) 172
EcoRII CCWGG 1 cut(s) 219
EgeI GGCGCC 1 cut(s) 428
EheI GGCGCC 1 cut(s) 428
FaeI CATG 2 cut(s) 265, 298
FaiI YATR 6 cut(s) 33, 263, 296, 306, 327, 423
FatI CATG 2 cut(s) 261, 294
FblI GTMKAC 1 cut(s) 215
Fnu4HI GCNGC 2 cut(s) 136, 237
FokI GGATG 2 cut(s) 404, 446
Fsp4HI GCNGC 2 cut(s) 136, 237
FspBI CTAG 1 cut(s) 149
GlaI GCGC 3 cut(s) 102, 398, 428
GluI GCNGC 2 cut(s) 136, 237
HaeII RGCGCY 3 cut(s) 104, 400, 430
HaeIII GGCC 1 cut(s) 161
HapII CCGG 2 cut(s) 79, 464
HhaI GCGC 3 cut(s) 103, 399, 429
Hin1I GRCGYC 1 cut(s) 427
Hin1II CATG 2 cut(s) 265, 298
Hin6I GCGC 3 cut(s) 101, 397, 427
HinP1I GCGC 3 cut(s) 101, 397, 427
HincII GTYRAC 1 cut(s) 216
HindII GTYRAC 1 cut(s) 216
HindIII AAGCTT 1 cut(s) 184
HinfI GANTC 1 cut(s) 442
HpaII CCGG 2 cut(s) 79, 464
HphI GGTGA 1 cut(s) 362
Hpy166II GTNNAC 1 cut(s) 216
Hpy188I TCNGA 1 cut(s) 123
Hpy188III TCNNGA 2 cut(s) 262, 295
Hpy8I GTNNAC 1 cut(s) 216
HpyAV CCTTC 2 cut(s) 76, 259
HpyCH4III ACNGT 1 cut(s) 384
HpyCH4V TGCA 5 cut(s) 62, 110, 138, 173, 454
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 141
Hsp92I GRCGYC 1 cut(s) 427
Hsp92II CATG 2 cut(s) 265, 298
HspAI GCGC 3 cut(s) 101, 397, 427
KasI GGCGCC 1 cut(s) 426
LpnPI CCDG 5 cut(s) 92, 206, 224, 233, 477
Lsp1109I GCAGC 2 cut(s) 122, 248
LweI GCATC 1 cut(s) 95
MaeI CTAG 1 cut(s) 149
MaeIII GTNAC 1 cut(s) 19
MboII GAAGA 5 cut(s) 136, 262, 377, 383, 479
MfeI CAATTG 1 cut(s) 433
MluCI AATT 4 cut(s) 301, 402, 433, 471
Mly113I GGCGCC 1 cut(s) 427
MmeI TCCRAC 1 cut(s) 419
MnlI CCTC 7 cut(s) 150, 217, 237, 262, 271, 291, 355
MroXI GAANNNNTTC 1 cut(s) 185
MspA1I CMGCKG 1 cut(s) 192
MspI CCGG 2 cut(s) 79, 464
MspR9I CCNGG 2 cut(s) 221, 464
MunI CAATTG 1 cut(s) 433
MvaI CCWGG 1 cut(s) 221
MwoI GCNNNNNNNGC 2 cut(s) 107, 141
NarI GGCGCC 1 cut(s) 427
NciI CCSGG 1 cut(s) 464
NlaIII CATG 2 cut(s) 265, 298
NlaIV GGNNCC 1 cut(s) 428
PagI TCATGA 2 cut(s) 261, 294
PdmI GAANNNNTTC 1 cut(s) 185
PfeI GAWTC 1 cut(s) 442
PfoI TCCNGGA 1 cut(s) 462
PkrI GCNGC 2 cut(s) 137, 238
PluTI GGCGCC 1 cut(s) 430
PsiI TTATAA 2 cut(s) 33, 306
Psp6I CCWGG 1 cut(s) 219
PspGI CCWGG 1 cut(s) 219
PspN4I GGNNCC 1 cut(s) 428
PspPI GGNCC 1 cut(s) 159
PstI CTGCAG 2 cut(s) 64, 140
SalI GTCGAC 1 cut(s) 214
SatI GCNGC 2 cut(s) 136, 237
Sau96I GGNCC 1 cut(s) 159
ScrFI CCNGG 2 cut(s) 221, 464
SetI ASST 3 cut(s) 137, 188, 248
SfaNI GCATC 1 cut(s) 95
SfcI CTRYAG 2 cut(s) 60, 136
SfoI GGCGCC 1 cut(s) 428
SpeI ACTAGT 1 cut(s) 148
Sse9I AATT 4 cut(s) 301, 402, 433, 471
SsiI CCGC 2 cut(s) 192, 412
SspDI GGCGCC 1 cut(s) 426
SspMI CTAG 1 cut(s) 149
StyD4I CCNGG 2 cut(s) 219, 462
TaaI ACNGT 1 cut(s) 384
TaqI TCGA 3 cut(s) 155, 215, 386
TasI AATT 4 cut(s) 301, 402, 433, 471
TfiI GAWTC 1 cut(s) 442
TscAI CASTG 4 cut(s) 169, 246, 337, 413
TseI GCWGC 2 cut(s) 135, 236
TspDTI ATGAA 2 cut(s) 278, 384
TspRI CASTG 4 cut(s) 169, 246, 337, 413
XapI RAATTY 1 cut(s) 471
XcmI CCANNNNNNNNNTGG 1 cut(s) 227
XmiI GTMKAC 1 cut(s) 215
XmnI GAANNNNTTC 1 cut(s) 185
XspI CTAG 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.