RLG00000013172

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004843
Physical Location & Seq
Forward (+)
27047169 .. 27049222
2054 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013172

Sequence Viewer

Length: 465 bp
ATGAGGGCCGCCTCTGTTTCAACGGTGAGCAGCCAGGAGGGTCTTAATGTACTTTCCCAGTCGATTCAGGAAGACAATGGGGTGGTGGGGATGAAGATTCTTGTAAAAAAGGAAGATTTGGAGCGAGTTTTCGAACTCATGAGGAATATTAGCAATACTAACCACAAAAACGATGACAATGGTGCTGATGAGGAGGATGAAGAAGCATTTTTGTCGATAGAGGAGCGCCTAAATCAGTTGCGCAAAAAGCATAGGCGCCATAATTCTTGGAGTCCTACTGGGGAAGATATGGAAGTTATCCATAGTGTCACAAAGCTGTTCGGACTGTTGTCAATTGAACTATGGAAGAGAAAGAGTGCATATATTCTGCGCCTCTGCGGAGTATCGATAGGAGATAAGAGTGATGATTATTTAGAGAAAGAGGCTATACATACACAGCTCATAAGCACTGAGCTCAATATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

17.55

Weight (kDa)

5.27

Isoelectric Point (pI)

54.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 242
AccB1I GGYRCC 1 cut(s) 255
AciI CCGC 2 cut(s) 9, 378
AcyI GRCGYC 1 cut(s) 256
AfaI GTAC 1 cut(s) 51
AgsI TTSAA 2 cut(s) 21, 338
AjnI CCWGG 1 cut(s) 33
AluBI AGCT 3 cut(s) 316, 439, 454
AluI AGCT 3 cut(s) 316, 439, 454
Alw21I GWGCWC 1 cut(s) 456
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 1 cut(s) 30
AspLEI GCGC 4 cut(s) 228, 243, 258, 372
AspS9I GGNCC 1 cut(s) 6
AsuHPI GGTGA 1 cut(s) 37
AsuII TTCGAA 1 cut(s) 132
BanI GGYRCC 1 cut(s) 255
BanII GRGCYC 1 cut(s) 456
BbsI GAAGAC 1 cut(s) 78
Bbv12I GWGCWC 1 cut(s) 456
BbvI GCAGC 1 cut(s) 42
BcgI CGANNNNNNTGC 2 cut(s) 195, 229
BciT130I CCWGG 1 cut(s) 35
BfoI RGCGCY 2 cut(s) 229, 259
BisI GCNGC 2 cut(s) 9, 31
BlsI GCNGC 2 cut(s) 10, 32
Bme1390I CCNGG 1 cut(s) 35
BmgT120I GGNCC 1 cut(s) 6
BmiI GGNNCC 1 cut(s) 257
BmrFI CCNGG 1 cut(s) 35
BmrI ACTGGG 2 cut(s) 52, 288
BmuI ACTGGG 2 cut(s) 52, 288
BoxI GACNNNNGTC 1 cut(s) 328
BpiI GAAGAC 1 cut(s) 78
Bpu14I TTCGAA 1 cut(s) 132
Bsa29I ATCGAT 1 cut(s) 386
BsaHI GRCGYC 1 cut(s) 256
Bse1I ACTGG 2 cut(s) 58, 283
BseBI CCWGG 1 cut(s) 35
BseCI ATCGAT 1 cut(s) 386
BseGI GGATG 2 cut(s) 96, 202
BseMII CTCAG 1 cut(s) 441
BseNI ACTGG 2 cut(s) 58, 283
BseRI GAGGAG 2 cut(s) 206, 236
BseXI GCAGC 1 cut(s) 42
BshFI GGCC 1 cut(s) 8
BshNI GGYRCC 1 cut(s) 255
BshVI ATCGAT 1 cut(s) 386
BsiHKAI GWGCWC 1 cut(s) 456
BsnI GGCC 1 cut(s) 8
Bsp119I TTCGAA 1 cut(s) 132
Bsp1286I GDGCHC 1 cut(s) 456
BspACI CCGC 2 cut(s) 9, 378
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 1 cut(s) 442
BspDI ATCGAT 1 cut(s) 386
BspHI TCATGA 1 cut(s) 138
BspLI GGNNCC 1 cut(s) 257
BspT104I TTCGAA 1 cut(s) 132
BspT107I GGYRCC 1 cut(s) 255
BsrI ACTGG 2 cut(s) 58, 283
BssNI GRCGYC 1 cut(s) 256
Bst2UI CCWGG 1 cut(s) 35
Bst4CI ACNGT 2 cut(s) 25, 327
Bst6I CTCTTC 1 cut(s) 341
BstACI GRCGYC 1 cut(s) 256
BstBI TTCGAA 1 cut(s) 132
BstDEI CTNAG 1 cut(s) 450
BstF5I GGATG 2 cut(s) 96, 202
BstH2I RGCGCY 2 cut(s) 229, 259
BstHHI GCGC 4 cut(s) 228, 243, 258, 372
BstMWI GCNNNNNNNGC 1 cut(s) 247
BstNI CCWGG 1 cut(s) 35
BstPAI GACNNNNGTC 1 cut(s) 328
BstSCI CCNGG 1 cut(s) 33
BstV1I GCAGC 1 cut(s) 42
BstV2I GAAGAC 1 cut(s) 78
Bsu15I ATCGAT 1 cut(s) 386
BsuRI GGCC 1 cut(s) 8
BsuTUI ATCGAT 1 cut(s) 386
BtsCI GGATG 2 cut(s) 96, 202
BtsIMutI CAGTG 1 cut(s) 447
CciI TCATGA 1 cut(s) 138
CfoI GCGC 4 cut(s) 228, 243, 258, 372
Cfr13I GGNCC 1 cut(s) 6
ClaI ATCGAT 1 cut(s) 386
Csp6I GTAC 1 cut(s) 50
CviAII CATG 1 cut(s) 139
CviJI RGCY 6 cut(s) 8, 33, 316, 425, 439, 454
CviKI_1 RGCY 6 cut(s) 8, 33, 316, 425, 439, 454
CviQI GTAC 1 cut(s) 50
DdeI CTNAG 1 cut(s) 450
DinI GGCGCC 1 cut(s) 257
Eam1104I CTCTTC 1 cut(s) 341
EarI CTCTTC 1 cut(s) 341
Ecl136II GAGCTC 1 cut(s) 454
Eco24I GRGCYC 1 cut(s) 456
Eco53kI GAGCTC 1 cut(s) 454
EcoICRI GAGCTC 1 cut(s) 454
EcoRII CCWGG 1 cut(s) 33
EcoT38I GRGCYC 1 cut(s) 456
EgeI GGCGCC 1 cut(s) 257
EheI GGCGCC 1 cut(s) 257
FaeI CATG 1 cut(s) 142
FatI CATG 1 cut(s) 138
Fnu4HI GCNGC 2 cut(s) 9, 31
FokI GGATG 2 cut(s) 103, 209
FriOI GRGCYC 1 cut(s) 456
Fsp4HI GCNGC 2 cut(s) 9, 31
FspI TGCGCA 1 cut(s) 242
GlaI GCGC 4 cut(s) 227, 242, 257, 371
GluI GCNGC 2 cut(s) 9, 31
HaeII RGCGCY 2 cut(s) 229, 259
HaeIII GGCC 1 cut(s) 8
HhaI GCGC 4 cut(s) 228, 243, 258, 372
Hin1I GRCGYC 1 cut(s) 256
Hin1II CATG 1 cut(s) 142
Hin6I GCGC 4 cut(s) 226, 241, 256, 370
HinP1I GCGC 4 cut(s) 226, 241, 256, 370
HinfI GANTC 3 cut(s) 64, 97, 271
HphI GGTGA 1 cut(s) 37
Hpy188I TCNGA 1 cut(s) 323
Hpy188III TCNNGA 2 cut(s) 68, 139
HpyCH4III ACNGT 2 cut(s) 25, 327
HpyCH4V TGCA 1 cut(s) 359
HpyF10VI GCNNNNNNNGC 1 cut(s) 247
HpyF3I CTNAG 1 cut(s) 450
Hsp92I GRCGYC 1 cut(s) 256
Hsp92II CATG 1 cut(s) 142
HspAI GCGC 4 cut(s) 226, 241, 256, 370
KasI GGCGCC 1 cut(s) 255
LmnI GCTCC 2 cut(s) 121, 223
LpnPI CCDG 5 cut(s) 20, 47, 53, 71, 264
Lsp1109I GCAGC 1 cut(s) 42
MaeIII GTNAC 1 cut(s) 307
MboII GAAGA 6 cut(s) 83, 106, 125, 212, 296, 358
MfeI CAATTG 1 cut(s) 333
MhlI GDGCHC 1 cut(s) 456
MluCI AATT 2 cut(s) 262, 333
Mly113I GGCGCC 1 cut(s) 256
MlyI GAGTC 1 cut(s) 280
MnlI CCTC 8 cut(s) 22, 31, 135, 184, 187, 214, 383, 415
MseI TTAA 1 cut(s) 45
MspR9I CCNGG 1 cut(s) 35
MunI CAATTG 1 cut(s) 333
MvaI CCWGG 1 cut(s) 35
MwoI GCNNNNNNNGC 1 cut(s) 247
NarI GGCGCC 1 cut(s) 256
NlaIII CATG 1 cut(s) 142
NlaIV GGNNCC 1 cut(s) 257
NmuCI GTSAC 1 cut(s) 307
NsbI TGCGCA 1 cut(s) 242
NspV TTCGAA 1 cut(s) 132
PagI TCATGA 1 cut(s) 138
PfeI GAWTC 2 cut(s) 64, 97
PkrI GCNGC 2 cut(s) 10, 32
PleI GAGTC 1 cut(s) 279
PluTI GGCGCC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 279
PshAI GACNNNNGTC 1 cut(s) 328
Psp124BI GAGCTC 1 cut(s) 456
Psp6I CCWGG 1 cut(s) 33
PspGI CCWGG 1 cut(s) 33
PspN4I GGNNCC 1 cut(s) 257
PspPI GGNCC 1 cut(s) 6
RsaI GTAC 1 cut(s) 51
RsaNI GTAC 1 cut(s) 50
SacI GAGCTC 1 cut(s) 456
SaqAI TTAA 1 cut(s) 45
SatI GCNGC 2 cut(s) 9, 31
Sau96I GGNCC 1 cut(s) 6
SchI GAGTC 1 cut(s) 280
ScrFI CCNGG 1 cut(s) 35
SduI GDGCHC 1 cut(s) 456
SetI ASST 3 cut(s) 318, 441, 456
SfoI GGCGCC 1 cut(s) 257
SfuI TTCGAA 1 cut(s) 132
SgeI CNNG 9 cut(s) 46, 47, 70, 80, 113, 137, 151, 279, 291
Sse9I AATT 2 cut(s) 262, 333
SsiI CCGC 2 cut(s) 9, 378
SspDI GGCGCC 1 cut(s) 255
SspI AATATT 1 cut(s) 148
SstI GAGCTC 1 cut(s) 456
StyD4I CCNGG 1 cut(s) 33
TaaI ACNGT 2 cut(s) 25, 327
TaqI TCGA 4 cut(s) 62, 132, 215, 386
TasI AATT 2 cut(s) 262, 333
TatI WGTACW 1 cut(s) 49
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 2 cut(s) 64, 97
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TscAI CASTG 1 cut(s) 454
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 1 cut(s) 30
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 2 cut(s) 107, 213
TspRI CASTG 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.