Rorug06G0121000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004843
Physical Location & Seq
Forward (+)
16630010 .. 16637022
7013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0121000.1

Sequence Viewer

Length: 2661 bp
ATGGCTCAGCCTCTCGTGAAGAAAGACGACGATCGCGACGATGAAGAGTACTCTCCATTTCTGGGGATTGAAAAGGGGGCTGTTCTTCAAGAAGCTAGGGTCTTCAATGATCCTCAGCTAGACGCAAGAAGATGCTCTCAGGTTATCACAAAGCTTCTGTATCTACTGAATCAGGGTGAAACCTTCACTAAGGTTGAAGCTACAGAAGTTTTCTTTTCGGTGACCAAGCTTTTCCAGTCGAGAGATATAGGATTGAGGAGAATGGTTTATCTGATGATAAAAGAGTTATCGCCTTCTGCTGATGAGGTTATTATTGTGACAAGCTCCCTAATGAAGGACATGAATAGTAAAACTGATATGTATCGGGCCAATGCCATTCGTGTGCTTTGTCGGATTACGGATGGAACCCTTCTTACCCAGATTGAGCGGTATTTGAAACAAGCCATTGTTGACAAGAATCCAGTTGTTGCGAGTGCAGCTCTAGTTAGTGGGATCCATCTACTCCAGACTAACCCAGAGATTGTTAAAAGGTGGAGTAATGAGGTTCAGGAAGCTGTCCAGTCAAGGGCAGCCCTTGTACAGTTTCATGCACTGGCTTTACTTCATCAGATAAGACAAAATGACCGTCTTGCTGTTAGCAAGTTGGTTACCAGCTTGACAAGGGGCACTGTCCGTTCTCCATTAGCTCAATGCCTGTTGATCCGCTATACTAGTCAGGTAATCCGGGAGTCCGCTGGGAGTACACAAGCAGGTGACCGTCCTTTTTATGATTATCTCGAGGGTTGCCTGCGGCACAAAGCAGAGATGGTAATTTTTGAAGCTGCCAGAGCAATTACAGAGCTCCATGGTGTGACCAACAGAGAATTGACTCCAGCTATCACCGTTCTTCAGCTATTTTTAAGTTCTTCAAAGCCAGTGTTAAGATTTGCTGCGGTCCGCACCCTAAACAAGGTGGCGATGACTCATCCAATGGCCGTCACAAACTGCAACATCGATATGGAGAGTTTGATTTCTGACCAGAACAGAAGCATTGCCACACTTGCGATCACCACACTTCTGAAGACAGGAAATGAATCAAGTGTGGATCGCTTGATGAAGCAGATAACTAACTTCATGTCAGATATTGCTGATGAATTCAAAATTGTAGTTGTGGAAGCCATTAGATCTCTGTGTTTGAAGTTTCCATTGAAGTATAGAGCTCTCATGAACTTCCTAAGCAACATTCTCCGAGAAGAAGGCGGGTTTGAGTACAAAAAAGCTATTGTTGACTCAATTGTGATTCTCATTAGAGATATCCCAGAAGCAAAAGAAAGTGGCTTGCTTCATCTCTGTGAGTTCATTGAAGATTGTGAATTTACCTACCTTTCTACTCAGATACTCCACTTTTTGGGGATTGAAGGGCCAAGAACCTCGGATCCAAGCAAATACATACGGTACATTTATAATCGAGTGCATCTTGAGAATGCCACTGTCCGGGCCAGTGCTGTGAGCACATTAGCCAAATTTGGTGCCATGGTTGATTCATTGAAGCCCCGAGTGTTTATTCTGTTAAGAAGGTGCCTCTTTGACAGTGATGATGAGGTTCGTGATAGGGCAACACTCTATCTTAGTACACTTGGAGGGGATGGTTGTGTTGTTGAGACTGACCAGGATGTAAAGGACTTTCTCTTTGGGTCTCTGGATGTCCCTCTCGTTAATCTTGAGACAAGTTTGAAGAATTATGAGGCTTCAGAAGAACCATTTGACATCAATTCTGTACCCAAGGAGATTAAGTCTCAGCCACTTGCTGAGAAAAAAGCCCAGAGTAAAAAGCCAACTGGTCTGGGTGCTCCTCCAAGTGGCCCTGCATCCACAGTCGATGCTTATGAGAAGATGCTTTCTTCTATTCCAGAGTTTTCAAACTTTGGGAAACTTTTCAAGTCCTCAGCACCTGTGGAGTTGACAGAGGCAGAAACCGAATATGCAGTTAACGTTGTCAAGCACATTTTTGATAGGCATGTTGTGTTCCAGTACAACTGCACCAACACCATTCCCGAGCAATTACTGGAAAATGTTATTGTTGCCGTTGATGCTTCTGATGCAGAGGAGTTTACTGAAGTGGGATCCAAGCCTCTCAGGTCTCTTCCTTATGATACACCTGGCCAGACCTTTTTGGCGTTTGAGAAGCCAGAAGGTGTCCCTGCAGTTGGAAAATTTTCAAACACATTGAGATTCATTGTTAAAGAGGTTGATCCAACCACTGGAGAGGCAGAGGATGATGGTGTAGAAGATGAATACCAGCTGGAGGACCTTGAGGTGGTTGCTGCTGATTATATTTTGAAGGATCAAGTACTCAATTTCAGAAATGCATGGGAAACCATGGGCCCAGATTGTGAGCGTGTAGATGAATATGGCCTGGGCCAAAGGGAGAGCTTGAATGAAGCAGTCTCTGCTGTCATCAGCCTCCTTGGAATGCAGCCCTGTGAGGGCACGGAGGTTATTGCCAGCAACTCAAGGTCCCACACTTGCTTATTGGCTGGTGTATACATAGGCAATGTGAAGGTCCTCGTGCGGTTGTCTTTTGGAATTGATAGTTCAAAGGAGGTTGCGATGAAGCTTGTGGTTAGATCTGAGGATGTGACTGTCAGTGATGCCATCCATGAGATTGTGGCCAGCGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

886

Amino Acids

98.62

Weight (kDa)

5.12

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 26 - 539 1.5e-141 Adaptin N terminal region
Cnd1 PF12717 122 - 217 1.5e-06 non-SMC mitotic condensation complex subunit 1
COP-gamma_platf PF08752 621 - 766 8.9e-55 Coatomer gamma subunit appendage platform subdomain
Coatomer_g_Cpla PF16381 769 - 883 1.4e-34 Coatomer subunit gamma-1 C-terminal appendage platform
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1443
AarI CACCTGC 1 cut(s) 740
Acc36I ACCTGC 1 cut(s) 740
AccB1I GGYRCC 2 cut(s) 1508, 1557
AccB7I CCANNNNNTGG 2 cut(s) 1387, 2240
AccBSI CCGCTC 1 cut(s) 427
AccI GTMKAC 1 cut(s) 2523
AccII CGCG 1 cut(s) 36
AciI CCGC 9 cut(s) 427, 703, 732, 790, 932, 937, 1239, 2551, 2655
AclI AACGTT 1 cut(s) 1971
AcoI YGGCCR 3 cut(s) 972, 2140, 2649
AcsI RAATTY 4 cut(s) 1133, 1352, 1502, 2192
AcuI CTGAAG 4 cut(s) 872, 1079, 1713, 2115
AfaI GTAC 9 cut(s) 50, 579, 742, 1250, 1436, 1612, 1758, 2012, 2331
AhlI ACTAGT 1 cut(s) 710
AjnI CCWGG 3 cut(s) 1647, 2137, 2394
AloI GAACNNNNNNTCC 2 cut(s) 1399, 1431
Alw21I GWGCWC 4 cut(s) 843, 1201, 1493, 1831
Alw26I GTCTC 6 cut(s) 1634, 1680, 1697, 1779, 2124, 2431
AlwNI CAGNNNCTG 2 cut(s) 1931, 2429
Ama87I CYCGRG 3 cut(s) 776, 1533, 2033
ApaI GGGCCC 1 cut(s) 2366
ApeKI GCWGC 6 cut(s) 476, 569, 821, 929, 2303, 2455
ApoI RAATTY 4 cut(s) 1133, 1352, 1502, 2192
ArsI GACNNNNNNTTYG 4 cut(s) 610, 642, 1652, 1684
Asp700I GAANNNNTTC 2 cut(s) 1913, 2194
AsuC2I CCSGG 2 cut(s) 725, 1475
AsuHPI GGTGA 5 cut(s) 188, 232, 764, 871, 1039
AvaI CYCGRG 3 cut(s) 776, 1533, 2033
AvaII GGWCC 4 cut(s) 934, 2287, 2496, 2542
BaeGI GKGCMC 3 cut(s) 668, 2366, 2471
BalI TGGCCA 2 cut(s) 2142, 2651
BamHI GGATCC 3 cut(s) 492, 1414, 2102
BanI GGYRCC 2 cut(s) 1508, 1557
BanII GRGCYC 3 cut(s) 843, 1201, 2366
BauI CACGAG 2 cut(s) 14, 2546
BbsI GAAGAC 2 cut(s) 94, 1067
Bbv12I GWGCWC 4 cut(s) 843, 1201, 1493, 1831
BbvCI CCTCAGC 2 cut(s) 114, 1924
BbvI GCAGC 6 cut(s) 488, 581, 808, 916, 2290, 2467
BccI CCATC 6 cut(s) 395, 504, 799, 1619, 2252, 2642
BceAI ACGGC 2 cut(s) 959, 2048
BciT130I CCWGG 3 cut(s) 1649, 2139, 2396
BcnI CCSGG 2 cut(s) 725, 1475
BcoDI GTCTC 6 cut(s) 1634, 1680, 1697, 1779, 2124, 2431
BcuI ACTAGT 1 cut(s) 710
BfaI CTAG 5 cut(s) 96, 119, 482, 711, 2659
BfmI CTRYAG 2 cut(s) 201, 2181
BfuAI ACCTGC 1 cut(s) 740
BglII AGATCT 2 cut(s) 1163, 2606
BisI GCNGC 8 cut(s) 477, 570, 791, 822, 930, 2304, 2456, 2656
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 8 cut(s) 478, 571, 792, 823, 931, 2305, 2457, 2657
BmcAI AGTACT 2 cut(s) 50, 2331
Bme1390I CCNGG 5 cut(s) 725, 1475, 1649, 2139, 2396
Bme18I GGWCC 4 cut(s) 934, 2287, 2496, 2542
BmeT110I CYCGRG 3 cut(s) 776, 1533, 2033
BmiI GGNNCC 8 cut(s) 406, 494, 1416, 1510, 1559, 2104, 2364, 2498
BmrFI CCNGG 5 cut(s) 725, 1475, 1649, 2139, 2396
BmsI GCATC 8 cut(s) 122, 1462, 1849, 1856, 1863, 2059, 2068, 2620
BpiI GAAGAC 2 cut(s) 94, 1067
BplI GAGNNNNNCTC 2 cut(s) 463, 495
BpmI CTGGAG 4 cut(s) 488, 855, 2262, 2303
Bpu10I CCTNAGC 3 cut(s) 114, 1214, 1924
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 4 cut(s) 1478, 1721, 2312, 2476
BpuMI CCSGG 2 cut(s) 725, 1475
Bsa29I ATCGAT 1 cut(s) 993
BsaBI GATNNNNATC 1 cut(s) 360
BsaI GGTCTC 2 cut(s) 1680, 2124
BsaJI CCNNGG 7 cut(s) 844, 1410, 1512, 1761, 2358, 2395, 2446
BsaXI ACNNNNNCTCC 2 cut(s) 1757, 1787
Bse3DI GCAATG 2 cut(s) 1029, 2539
Bse8I GATNNNNATC 1 cut(s) 360
BseBI CCWGG 3 cut(s) 1649, 2139, 2396
BseCI ATCGAT 1 cut(s) 993
BseDI CCNNGG 7 cut(s) 844, 1410, 1512, 1761, 2358, 2395, 2446
BseGI GGATG 9 cut(s) 406, 964, 1630, 1657, 1687, 1847, 2260, 2620, 2634
BseJI GATNNNNATC 1 cut(s) 360
BseMI GCAATG 2 cut(s) 1029, 2539
BseMII CTCAG 9 cut(s) 20, 128, 152, 1385, 1779, 1790, 1938, 2128, 2601
BseRI GAGGAG 3 cut(s) 271, 1821, 2099
BseSI GKGCMC 3 cut(s) 668, 2366, 2471
BseXI GCAGC 6 cut(s) 488, 581, 808, 916, 2290, 2467
BseYI CCCAGC 1 cut(s) 734
BsgI GTGCAG 2 cut(s) 495, 2002
Bsh1236I CGCG 1 cut(s) 36
Bsh1285I CGRYCG 1 cut(s) 34
BshNI GGYRCC 2 cut(s) 1508, 1557
BshVI ATCGAT 1 cut(s) 993
BsiEI CGRYCG 1 cut(s) 34
BsiHKAI GWGCWC 4 cut(s) 843, 1201, 1493, 1831
BsiHKCI CYCGRG 3 cut(s) 776, 1533, 2033
BsiSI CCGG 2 cut(s) 724, 1474
BslFI GGGAC 3 cut(s) 1670, 2162, 2482
BsmAI GTCTC 6 cut(s) 1634, 1680, 1697, 1779, 2124, 2431
BsmFI GGGAC 3 cut(s) 1670, 2162, 2482
BsmI GAATGC 2 cut(s) 1468, 2457
Bso31I GGTCTC 2 cut(s) 1680, 2124
BsoBI CYCGRG 3 cut(s) 776, 1533, 2033
Bsp120I GGGCCC 1 cut(s) 2362
Bsp1286I GDGCHC 7 cut(s) 668, 843, 1201, 1493, 1831, 2366, 2471
Bsp1407I TGTACA 1 cut(s) 577
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 3 cut(s) 844, 1512, 2358
Bsp68I TCGCGA 1 cut(s) 36
BspACI CCGC 9 cut(s) 427, 703, 732, 790, 932, 937, 1239, 2551, 2655
BspCNI CTCAG 9 cut(s) 19, 127, 151, 1384, 1780, 1789, 1937, 2127, 2602
BspDI ATCGAT 1 cut(s) 993
BspFNI CGCG 1 cut(s) 36
BspHI TCATGA 1 cut(s) 1203
BspLI GGNNCC 8 cut(s) 406, 494, 1416, 1510, 1559, 2104, 2364, 2498
BspMAI CTGCAG 1 cut(s) 2185
BspMI ACCTGC 1 cut(s) 740
BspT107I GGYRCC 2 cut(s) 1508, 1557
BspTNI GGTCTC 2 cut(s) 1680, 2124
BsrBI CCGCTC 1 cut(s) 427
BsrDI GCAATG 2 cut(s) 1029, 2539
BsrGI TGTACA 1 cut(s) 577
BssECI CCNNGG 7 cut(s) 844, 1410, 1512, 1761, 2358, 2395, 2446
BssNAI GTATAC 1 cut(s) 2524
BssSI CACGAG 2 cut(s) 14, 2546
BssT1I CCWWGG 5 cut(s) 844, 1512, 1761, 2358, 2446
Bst1107I GTATAC 1 cut(s) 2524
Bst2BI CACGAG 2 cut(s) 14, 2546
Bst2UI CCWGG 3 cut(s) 1649, 2139, 2396
Bst6I CTCTTC 2 cut(s) 39, 2127
BstAPI GCANNNNNTGC 1 cut(s) 2429
BstAUI TGTACA 1 cut(s) 577
BstC8I GCNNGC 4 cut(s) 788, 1319, 2485, 2653
BstDSI CCRYGG 3 cut(s) 844, 1512, 2358
BstEII GGTNACC 3 cut(s) 220, 646, 752
BstENI CCTNNNNNAGG 2 cut(s) 332, 947
BstF5I GGATG 9 cut(s) 406, 964, 1630, 1657, 1687, 1847, 2260, 2620, 2634
BstFNI CGCG 1 cut(s) 36
BstMAI GTCTC 6 cut(s) 1634, 1680, 1697, 1779, 2124, 2431
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 6 cut(s) 476, 827, 1040, 2069, 2078, 2429
BstNI CCWGG 3 cut(s) 1649, 2139, 2396
BstNSI RCATGY 1 cut(s) 2000
BstPI GGTNACC 3 cut(s) 220, 646, 752
BstSCI CCNGG 5 cut(s) 723, 1473, 1647, 2137, 2394
BstSFI CTRYAG 2 cut(s) 201, 2181
BstSLI GKGCMC 3 cut(s) 668, 2366, 2471
BstUI CGCG 1 cut(s) 36
BstV1I GCAGC 6 cut(s) 488, 581, 808, 916, 2290, 2467
BstV2I GAAGAC 2 cut(s) 94, 1067
BstX2I RGATCY 5 cut(s) 492, 1163, 1414, 2102, 2606
BstYI RGATCY 5 cut(s) 492, 1163, 1414, 2102, 2606
BstZ17I GTATAC 1 cut(s) 2524
Bsu15I ATCGAT 1 cut(s) 993
BsuTUI ATCGAT 1 cut(s) 993
BtgI CCRYGG 3 cut(s) 844, 1512, 2358
BtgZI GCGATG 2 cut(s) 971, 2603
BtsCI GGATG 9 cut(s) 406, 964, 1630, 1657, 1687, 1847, 2260, 2620, 2634
BtsIMutI CAGTG 8 cut(s) 590, 666, 921, 1467, 1486, 1576, 2238, 2632
BtuMI TCGCGA 1 cut(s) 36
BveI ACCTGC 1 cut(s) 740
Cac8I GCNNGC 4 cut(s) 788, 1319, 2485, 2653
CaiI CAGNNNCTG 2 cut(s) 1931, 2429
CciI TCATGA 1 cut(s) 1203
ClaI ATCGAT 1 cut(s) 993
CpoI CGGWCCG 1 cut(s) 934
CseI GACGC 1 cut(s) 131
Csp6I GTAC 9 cut(s) 49, 578, 741, 1249, 1435, 1611, 1757, 2011, 2330
CspI CGGWCCG 1 cut(s) 934
CviQI GTAC 9 cut(s) 49, 578, 741, 1249, 1435, 1611, 1757, 2011, 2330
EaeI YGGCCR 3 cut(s) 972, 2140, 2649
Eam1104I CTCTTC 2 cut(s) 39, 2127
EarI CTCTTC 2 cut(s) 39, 2127
Ecl136II GAGCTC 2 cut(s) 841, 1199
Eco130I CCWWGG 5 cut(s) 844, 1512, 1761, 2358, 2446
Eco24I GRGCYC 3 cut(s) 843, 1201, 2366
Eco31I GGTCTC 2 cut(s) 1680, 2124
Eco32I GATATC 1 cut(s) 1294
Eco47I GGWCC 4 cut(s) 934, 2287, 2496, 2542
Eco53kI GAGCTC 2 cut(s) 841, 1199
Eco57I CTGAAG 4 cut(s) 872, 1079, 1713, 2115
Eco88I CYCGRG 3 cut(s) 776, 1533, 2033
Eco91I GGTNACC 3 cut(s) 220, 646, 752
EcoICRI GAGCTC 2 cut(s) 841, 1199
EcoNI CCTNNNNNAGG 2 cut(s) 332, 947
EcoO109I RGGNCCY 3 cut(s) 2287, 2496, 2542
EcoO65I GGTNACC 3 cut(s) 220, 646, 752
EcoRI GAATTC 1 cut(s) 1133
EcoRII CCWGG 3 cut(s) 1647, 2137, 2394
EcoRV GATATC 1 cut(s) 1294
EcoT14I CCWWGG 5 cut(s) 844, 1512, 1761, 2358, 2446
EcoT22I ATGCAT 1 cut(s) 2350
EcoT38I GRGCYC 3 cut(s) 843, 1201, 2366
ErhI CCWWGG 5 cut(s) 844, 1512, 1761, 2358, 2446
FaqI GGGAC 3 cut(s) 1670, 2162, 2482
FauI CCCGC 1 cut(s) 1232
FblI GTMKAC 1 cut(s) 2523
Fnu4HI GCNGC 8 cut(s) 477, 570, 791, 822, 930, 2304, 2456, 2656
FokI GGATG 9 cut(s) 413, 951, 1637, 1664, 1694, 1834, 2267, 2621, 2627
FriOI GRGCYC 3 cut(s) 843, 1201, 2366
Fsp4HI GCNGC 8 cut(s) 477, 570, 791, 822, 930, 2304, 2456, 2656
FspBI CTAG 5 cut(s) 96, 119, 482, 711, 2659
GluI GCNGC 8 cut(s) 477, 570, 791, 822, 930, 2304, 2456, 2656
GsaI CCCAGC 1 cut(s) 738
GsuI CTGGAG 4 cut(s) 488, 855, 2262, 2303
HapII CCGG 2 cut(s) 724, 1474
HgaI GACGC 1 cut(s) 131
HincII GTYRAC 4 cut(s) 451, 1267, 1941, 1969
HindII GTYRAC 4 cut(s) 451, 1267, 1941, 1969
HindIII AAGCTT 3 cut(s) 152, 227, 2594
HpaI GTTAAC 1 cut(s) 1969
HpaII CCGG 2 cut(s) 724, 1474
HphI GGTGA 5 cut(s) 188, 232, 764, 871, 1039
Hpy166II GTNNAC 8 cut(s) 451, 743, 1267, 1613, 1941, 1969, 2091, 2524
Hpy8I GTNNAC 8 cut(s) 451, 743, 1267, 1613, 1941, 1969, 2091, 2524
Hpy99I CGWCG 2 cut(s) 32, 41
HpyCH4IV ACGT 1 cut(s) 1971
HpyF10VI GCNNNNNNNGC 6 cut(s) 476, 827, 1040, 2069, 2078, 2429
HpySE526I ACGT 1 cut(s) 1971
KspAI GTTAAC 1 cut(s) 1969
LmnI GCTCC 3 cut(s) 329, 846, 1834
Lsp1109I GCAGC 6 cut(s) 488, 581, 808, 916, 2290, 2467
LweI GCATC 8 cut(s) 122, 1462, 1849, 1856, 1863, 2059, 2068, 2620
MaeI CTAG 5 cut(s) 96, 119, 482, 711, 2659
MaeII ACGT 1 cut(s) 1971
MaeIII GTNAC 7 cut(s) 220, 316, 646, 752, 850, 976, 2617
MbiI CCGCTC 1 cut(s) 427
MfeI CAATTG 1 cut(s) 1272
MflI RGATCY 5 cut(s) 492, 1163, 1414, 2102, 2606
MhlI GDGCHC 7 cut(s) 668, 843, 1201, 1493, 1831, 2366, 2471
MlsI TGGCCA 2 cut(s) 2142, 2651
MluNI TGGCCA 2 cut(s) 2142, 2651
MlyI GAGTC 4 cut(s) 737, 862, 955, 1262
MmeI TCCRAC 3 cut(s) 371, 2167, 2258
Mox20I TGGCCA 2 cut(s) 2142, 2651
Mph1103I ATGCAT 1 cut(s) 2350
MroXI GAANNNNTTC 2 cut(s) 1913, 2194
MscI TGGCCA 2 cut(s) 2142, 2651
MseI TTAA 8 cut(s) 525, 899, 920, 1550, 1695, 1770, 1968, 2220
MslI CAYNNNNRTG 3 cut(s) 380, 995, 1329
Msp20I TGGCCA 2 cut(s) 2142, 2651
MspA1I CMGCKG 3 cut(s) 734, 2281, 2655
MspI CCGG 2 cut(s) 724, 1474
MspR9I CCNGG 5 cut(s) 725, 1475, 1649, 2139, 2396
MunI CAATTG 1 cut(s) 1272
Mva1269I GAATGC 2 cut(s) 1468, 2457
MvaI CCWGG 3 cut(s) 1649, 2139, 2396
MvnI CGCG 1 cut(s) 36
MwoI GCNNNNNNNGC 6 cut(s) 476, 827, 1040, 2069, 2078, 2429
NciI CCSGG 2 cut(s) 725, 1475
NcoI CCATGG 3 cut(s) 844, 1512, 2358
NlaIV GGNNCC 8 cut(s) 406, 494, 1416, 1510, 1559, 2104, 2364, 2498
NmuCI GTSAC 6 cut(s) 220, 316, 752, 850, 976, 2617
NruI TCGCGA 1 cut(s) 36
NsiI ATGCAT 1 cut(s) 2350
NspI RCATGY 1 cut(s) 2000
PaeR7I CTCGAG 1 cut(s) 776
PagI TCATGA 1 cut(s) 1203
PaqCI CACCTGC 1 cut(s) 740
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 36
PctI GAATGC 2 cut(s) 1468, 2457
PdmI GAANNNNTTC 2 cut(s) 1913, 2194
PfeI GAWTC 6 cut(s) 169, 457, 1073, 1279, 1520, 2211
PflMI CCANNNNNTGG 2 cut(s) 1387, 2240
PfoI TCCNGGA 1 cut(s) 723
PkrI GCNGC 8 cut(s) 478, 571, 792, 823, 931, 2305, 2457, 2657
Ple19I CGATCG 1 cut(s) 34
PleI GAGTC 4 cut(s) 736, 862, 955, 1262
PpsI GAGTC 4 cut(s) 736, 862, 955, 1262
PpuMI RGGWCCY 3 cut(s) 2287, 2496, 2542
PsiI TTATAA 1 cut(s) 1443
Psp124BI GAGCTC 2 cut(s) 843, 1201
Psp1406I AACGTT 1 cut(s) 1971
Psp5II RGGWCCY 3 cut(s) 2287, 2496, 2542
Psp6I CCWGG 3 cut(s) 1647, 2137, 2394
PspEI GGTNACC 3 cut(s) 220, 646, 752
PspFI CCCAGC 1 cut(s) 734
PspGI CCWGG 3 cut(s) 1647, 2137, 2394
PspN4I GGNNCC 8 cut(s) 406, 494, 1416, 1510, 1559, 2104, 2364, 2498
PspOMI GGGCCC 1 cut(s) 2362
PspPPI RGGWCCY 3 cut(s) 2287, 2496, 2542
PsrI GAACNNNNNNTAC 2 cut(s) 397, 429
PstI CTGCAG 1 cut(s) 2185
PstNI CAGNNNCTG 2 cut(s) 1931, 2429
PsuI RGATCY 5 cut(s) 492, 1163, 1414, 2102, 2606
PvuI CGATCG 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 2281
RruI TCGCGA 1 cut(s) 36
RsaI GTAC 9 cut(s) 50, 579, 742, 1250, 1436, 1612, 1758, 2012, 2331
RsaNI GTAC 9 cut(s) 49, 578, 741, 1249, 1435, 1611, 1757, 2011, 2330
RseI CAYNNNNRTG 3 cut(s) 380, 995, 1329
Rsr2I CGGWCCG 1 cut(s) 934
RsrII CGGWCCG 1 cut(s) 934
SacI GAGCTC 2 cut(s) 843, 1201
SaqAI TTAA 8 cut(s) 525, 899, 920, 1550, 1695, 1770, 1968, 2220
SatI GCNGC 8 cut(s) 477, 570, 791, 822, 930, 2304, 2456, 2656
ScaI AGTACT 2 cut(s) 50, 2331
SchI GAGTC 4 cut(s) 737, 862, 955, 1262
ScrFI CCNGG 5 cut(s) 725, 1475, 1649, 2139, 2396
SduI GDGCHC 7 cut(s) 668, 843, 1201, 1493, 1831, 2366, 2471
SfaNI GCATC 8 cut(s) 122, 1462, 1849, 1856, 1863, 2059, 2068, 2620
SfcI CTRYAG 2 cut(s) 201, 2181
Sfr274I CTCGAG 1 cut(s) 776
SinI GGWCC 4 cut(s) 934, 2287, 2496, 2542
SlaI CTCGAG 1 cut(s) 776
SmiMI CAYNNNNRTG 3 cut(s) 380, 995, 1329
SmlI CTYRAG 5 cut(s) 776, 1457, 1700, 2291, 2491
SmoI CTYRAG 5 cut(s) 776, 1457, 1700, 2291, 2491
SpeI ACTAGT 1 cut(s) 710
SsiI CCGC 9 cut(s) 427, 703, 732, 790, 932, 937, 1239, 2551, 2655
SspMI CTAG 5 cut(s) 96, 119, 482, 711, 2659
SstI GAGCTC 2 cut(s) 843, 1201
StyD4I CCNGG 5 cut(s) 723, 1473, 1647, 2137, 2394
StyI CCWWGG 5 cut(s) 844, 1512, 1761, 2358, 2446
TaiI ACGT 1 cut(s) 1974
TaqI TCGA 5 cut(s) 239, 777, 993, 1447, 1857
TatI WGTACW 7 cut(s) 48, 577, 740, 1248, 1610, 2010, 2329
TauI GCSGC 2 cut(s) 793, 2658
TfiI GAWTC 6 cut(s) 169, 457, 1073, 1279, 1520, 2211
Tru1I TTAA 8 cut(s) 525, 899, 920, 1550, 1695, 1770, 1968, 2220
Tru9I TTAA 8 cut(s) 525, 899, 920, 1550, 1695, 1770, 1968, 2220
TscAI CASTG 8 cut(s) 597, 673, 921, 1474, 1486, 1576, 2245, 2632
TseFI GTSAC 6 cut(s) 220, 316, 752, 850, 976, 2617
TseI GCWGC 6 cut(s) 476, 569, 821, 929, 2303, 2455
Tsp45I GTSAC 6 cut(s) 220, 316, 752, 850, 976, 2617
TspGWI ACGGA 3 cut(s) 413, 662, 2486
TspRI CASTG 8 cut(s) 597, 673, 921, 1474, 1486, 1576, 2245, 2632
Van91I CCANNNNNTGG 2 cut(s) 1387, 2240
VpaK11BI GGWCC 4 cut(s) 934, 2287, 2496, 2542
XagI CCTNNNNNAGG 2 cut(s) 332, 947
XapI RAATTY 4 cut(s) 1133, 1352, 1502, 2192
XceI RCATGY 1 cut(s) 2000
XhoI CTCGAG 1 cut(s) 776
XmiI GTMKAC 1 cut(s) 2523
XmnI GAANNNNTTC 2 cut(s) 1913, 2194
XspI CTAG 5 cut(s) 96, 119, 482, 711, 2659
ZrmI AGTACT 2 cut(s) 50, 2331
Zsp2I ATGCAT 1 cut(s) 2350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.