FvH4_2g15721

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004843
Physical Location & Seq
Reverse (-)
13789944 .. 13790408
465 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g15721.t1

Sequence Viewer

Length: 465 bp
ATGGAAGTGATCAAGTCCAAGTCATCATCATCATCGTCTGCAGAAGGAAAGCAAAGCCGAAGGGCGTCACTAAAGAAGGCATTCTTTCAGCATTTCAGGTCAGCTCGCGTTCATCAAAAGAACAAGAGCAGTAGGAACAATCTATTAATGAGGGCCGCCTCCATTTCAAAGGTGAGCAGCCAGGAGGGTTTTAATGTACTTGCCCACTCGACGTCTCAGGATGACAATGGGGTGTTGAGGATGAAGATTCTCGTGAAAAAGGAAGATTTGGAGAGAGTTTTCGAACTCATGAGGAATAATAATAACAATACTAACCACAAAAAAGATGAGAATGGTGCTGATGATGAGGAGGATGAAGAAGCATTTTTGTTGATAGAGGAGCGCCTAAATCAGTTGCGGAAAAAGCATAGGCGCCATTGTTCTTGGAGTCCTGCTCTGCAAACCATCCCAGAAGACATATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

17.77

Weight (kDa)

9.58

Isoelectric Point (pI)

60.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 215
AccB1I GGYRCC 1 cut(s) 411
AccII CGCG 1 cut(s) 108
AciI CCGC 2 cut(s) 156, 397
AcyI GRCGYC 3 cut(s) 65, 212, 412
AfaI GTAC 1 cut(s) 198
AgsI TTSAA 1 cut(s) 168
AjnI CCWGG 1 cut(s) 180
AluBI AGCT 1 cut(s) 104
AluI AGCT 1 cut(s) 104
Alw26I GTCTC 1 cut(s) 219
AoxI GGCC 1 cut(s) 153
ApeKI GCWGC 1 cut(s) 177
AseI ATTAAT 1 cut(s) 146
Asp700I GAANNNNTTC 1 cut(s) 80
AspLEI GCGC 2 cut(s) 384, 414
AspS9I GGNCC 1 cut(s) 153
AsuHPI GGTGA 1 cut(s) 184
AsuII TTCGAA 1 cut(s) 282
BanI GGYRCC 1 cut(s) 411
BauI CACGAG 1 cut(s) 251
BbsI GAAGAC 1 cut(s) 459
BbvI GCAGC 1 cut(s) 189
BccI CCATC 1 cut(s) 452
BciT130I CCWGG 1 cut(s) 182
BclI TGATCA 1 cut(s) 9
BcoDI GTCTC 1 cut(s) 219
BfmI CTRYAG 1 cut(s) 39
BfoI RGCGCY 2 cut(s) 385, 415
BisI GCNGC 2 cut(s) 156, 178
BlsI GCNGC 2 cut(s) 157, 179
Bme1390I CCNGG 1 cut(s) 182
BmgT120I GGNCC 1 cut(s) 153
BmiI GGNNCC 1 cut(s) 413
BmrFI CCNGG 1 cut(s) 182
BpiI GAAGAC 1 cut(s) 459
BplI GAGNNNNNCTC 2 cut(s) 418, 450
Bpu14I TTCGAA 1 cut(s) 282
BsaHI GRCGYC 3 cut(s) 65, 212, 412
BseBI CCWGG 1 cut(s) 182
BseGI GGATG 4 cut(s) 226, 246, 358, 444
BseMII CTCAG 1 cut(s) 230
BseRI GAGGAG 2 cut(s) 362, 392
BseXI GCAGC 1 cut(s) 189
Bsh1236I CGCG 1 cut(s) 108
BshFI GGCC 1 cut(s) 155
BshNI GGYRCC 1 cut(s) 411
BsmAI GTCTC 1 cut(s) 219
BsmBI CGTCTC 1 cut(s) 219
BsmI GAATGC 1 cut(s) 80
BsnI GGCC 1 cut(s) 155
Bsp119I TTCGAA 1 cut(s) 282
Bsp143I GATC 1 cut(s) 9
BspACI CCGC 2 cut(s) 156, 397
BspANI GGCC 1 cut(s) 155
BspCNI CTCAG 1 cut(s) 229
BspFNI CGCG 1 cut(s) 108
BspHI TCATGA 1 cut(s) 288
BspLI GGNNCC 1 cut(s) 413
BspMAI CTGCAG 1 cut(s) 43
BspT104I TTCGAA 1 cut(s) 282
BspT107I GGYRCC 1 cut(s) 411
BssMI GATC 1 cut(s) 9
BssNI GRCGYC 3 cut(s) 65, 212, 412
BssSI CACGAG 1 cut(s) 251
Bst2BI CACGAG 1 cut(s) 251
Bst2UI CCWGG 1 cut(s) 182
BstACI GRCGYC 3 cut(s) 65, 212, 412
BstBI TTCGAA 1 cut(s) 282
BstC8I GCNNGC 1 cut(s) 106
BstDEI CTNAG 1 cut(s) 216
BstF5I GGATG 4 cut(s) 226, 246, 358, 444
BstFNI CGCG 1 cut(s) 108
BstH2I RGCGCY 2 cut(s) 385, 415
BstHHI GCGC 2 cut(s) 384, 414
BstKTI GATC 1 cut(s) 12
BstMAI GTCTC 1 cut(s) 219
BstMBI GATC 1 cut(s) 9
BstMWI GCNNNNNNNGC 1 cut(s) 403
BstNI CCWGG 1 cut(s) 182
BstSCI CCNGG 1 cut(s) 180
BstSFI CTRYAG 1 cut(s) 39
BstUI CGCG 1 cut(s) 108
BstV1I GCAGC 1 cut(s) 189
BstV2I GAAGAC 1 cut(s) 459
BsuRI GGCC 1 cut(s) 155
BtsCI GGATG 4 cut(s) 226, 246, 358, 444
Cac8I GCNNGC 1 cut(s) 106
CciI TCATGA 1 cut(s) 288
CfoI GCGC 2 cut(s) 384, 414
Cfr13I GGNCC 1 cut(s) 153
CseI GACGC 1 cut(s) 54
Csp6I GTAC 1 cut(s) 197
CviAII CATG 1 cut(s) 289
CviJI RGCY 4 cut(s) 57, 104, 155, 180
CviKI_1 RGCY 4 cut(s) 57, 104, 155, 180
CviQI GTAC 1 cut(s) 197
DdeI CTNAG 1 cut(s) 216
DinI GGCGCC 1 cut(s) 413
DpnI GATC 1 cut(s) 11
DpnII GATC 1 cut(s) 9
EcoRII CCWGG 1 cut(s) 180
EgeI GGCGCC 1 cut(s) 413
EheI GGCGCC 1 cut(s) 413
Esp3I CGTCTC 1 cut(s) 219
FaeI CATG 1 cut(s) 292
FaiI YATR 3 cut(s) 290, 408, 458
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FatI CATG 1 cut(s) 288
FbaI TGATCA 1 cut(s) 9
Fnu4HI GCNGC 2 cut(s) 156, 178
FokI GGATG 4 cut(s) 233, 253, 365, 431
Fsp4HI GCNGC 2 cut(s) 156, 178
GlaI GCGC 2 cut(s) 383, 413
GluI GCNGC 2 cut(s) 156, 178
HaeII RGCGCY 2 cut(s) 385, 415
HaeIII GGCC 1 cut(s) 155
HgaI GACGC 1 cut(s) 54
HhaI GCGC 2 cut(s) 384, 414
Hin1I GRCGYC 3 cut(s) 65, 212, 412
Hin1II CATG 1 cut(s) 292
Hin6I GCGC 2 cut(s) 382, 412
HinP1I GCGC 2 cut(s) 382, 412
HinfI GANTC 2 cut(s) 247, 427
HphI GGTGA 1 cut(s) 184
Hpy188III TCNNGA 3 cut(s) 218, 253, 289
Hpy99I CGWCG 1 cut(s) 214
HpyAV CCTTC 3 cut(s) 38, 54, 70
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 2 cut(s) 41, 439
HpyF10VI GCNNNNNNNGC 1 cut(s) 403
HpyF3I CTNAG 1 cut(s) 216
HpySE526I ACGT 1 cut(s) 212
Hsp92I GRCGYC 3 cut(s) 65, 212, 412
Hsp92II CATG 1 cut(s) 292
HspAI GCGC 2 cut(s) 382, 412
KasI GGCGCC 1 cut(s) 411
Ksp22I TGATCA 1 cut(s) 9
Kzo9I GATC 1 cut(s) 9
LmnI GCTCC 1 cut(s) 379
LpnPI CCDG 5 cut(s) 82, 167, 194, 203, 444
Lsp1109I GCAGC 1 cut(s) 189
MaeII ACGT 1 cut(s) 212
MaeIII GTNAC 1 cut(s) 66
MalI GATC 1 cut(s) 11
MboI GATC 1 cut(s) 9
MboII GAAGA 4 cut(s) 256, 275, 368, 464
Mly113I GGCGCC 1 cut(s) 412
MlyI GAGTC 1 cut(s) 436
MnlI CCTC 8 cut(s) 144, 169, 178, 231, 285, 340, 343, 370
MroXI GAANNNNTTC 1 cut(s) 80
MseI TTAA 2 cut(s) 146, 192
MspR9I CCNGG 1 cut(s) 182
Mva1269I GAATGC 1 cut(s) 80
MvaI CCWGG 1 cut(s) 182
MvnI CGCG 1 cut(s) 108
MwoI GCNNNNNNNGC 1 cut(s) 403
NarI GGCGCC 1 cut(s) 412
NdeII GATC 1 cut(s) 9
NlaIII CATG 1 cut(s) 292
NlaIV GGNNCC 1 cut(s) 413
NmuCI GTSAC 1 cut(s) 66
NspV TTCGAA 1 cut(s) 282
PagI TCATGA 1 cut(s) 288
PctI GAATGC 1 cut(s) 80
PdmI GAANNNNTTC 1 cut(s) 80
PfeI GAWTC 1 cut(s) 247
PkrI GCNGC 2 cut(s) 157, 179
PleI GAGTC 1 cut(s) 435
PluTI GGCGCC 1 cut(s) 415
PpsI GAGTC 1 cut(s) 435
PshBI ATTAAT 1 cut(s) 146
Psp6I CCWGG 1 cut(s) 180
PspGI CCWGG 1 cut(s) 180
PspN4I GGNNCC 1 cut(s) 413
PspPI GGNCC 1 cut(s) 153
PstI CTGCAG 1 cut(s) 43
RsaI GTAC 1 cut(s) 198
RsaNI GTAC 1 cut(s) 197
SaqAI TTAA 2 cut(s) 146, 192
SatI GCNGC 2 cut(s) 156, 178
Sau3AI GATC 1 cut(s) 9
Sau96I GGNCC 1 cut(s) 153
SchI GAGTC 1 cut(s) 436
ScrFI CCNGG 1 cut(s) 182
SetI ASST 4 cut(s) 101, 106, 174, 215
SfcI CTRYAG 1 cut(s) 39
SfoI GGCGCC 1 cut(s) 413
SfuI TTCGAA 1 cut(s) 282
SsiI CCGC 2 cut(s) 156, 397
SspDI GGCGCC 1 cut(s) 411
StyD4I CCNGG 1 cut(s) 180
TaiI ACGT 1 cut(s) 215
TaqI TCGA 2 cut(s) 209, 282
TatI WGTACW 1 cut(s) 196
TauI GCSGC 1 cut(s) 158
TfiI GAWTC 1 cut(s) 247
Tru1I TTAA 2 cut(s) 146, 192
Tru9I TTAA 2 cut(s) 146, 192
TseFI GTSAC 1 cut(s) 66
TseI GCWGC 1 cut(s) 177
Tsp45I GTSAC 1 cut(s) 66
TspDTI ATGAA 3 cut(s) 101, 257, 369
VspI ATTAAT 1 cut(s) 146
XmnI GAANNNNTTC 1 cut(s) 80
ZraI GACGTC 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.