FvH4_2g20930

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003318
Physical Location & Seq
Forward (+)
17478308 .. 17480959
2652 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g20930.t1

Sequence Viewer

Length: 1350 bp
ATGGCTGTAATGAGCTCTTCATTGCTGTTATCTGTTCTTCTTGTTTTCTCTTTGAGTTCTTCATGCTATGGTAGCAGCTTCCAAGAGAACTCATTACTCCATAGTTATGTCGATGATGATAATCGTGAAGAAACTGGGTTTTATGGTTCCGAGCCTTCTCATCCTTCGTACATGAGCATCTTTGAGAATCCCAAGTTCTCCGAATCGAACAATCTCCGAGCTCGGCTCTTCAGCACATCAGCTTCCGTTAAACAGGTTAGCGTCGATGATTTTGGCGCTAAAGGAAATGGTGCTGCTGATGACACACAGGCATTTGTAAAGGCATGGAAGACTGCTTGTAGTTCCACTGGGGCACCTGTTCTTTTGGTGCCAAAGAAGAGCTATCTTGTTAAGCCAATCACATTTTCAGGGCCATGCAAATCTCAGCTTACAATGCAGATATATGGAACCATAGAAGCATCTGGTGACAGGTCAGTCTACAGCAAAGATCTAAATCACTGGATCATGTTTGACAACGTTAAAAATCTGTTGGTTCAAGGTCCTGGAACCATCAATGGCAACGGTCAAGTCTGGTGGAAAAACTCTTGCAAAAGAAAAGAAACTAAGCCCTGCGGTACGCACGCACCTACGGCTGTGACATTCTACAAGTGTAATAACTTGGTCGTGAAGAATCTTAAGTTTCAAGATGCACAGCAGATGCACGTCAGTTTCGAAGGGTGCACTAATGTTCAAGCTTCGTATCTAACAGTAACTGCGCCGGAGACTAGTCCCAACACTGACGGCATTCATATTACCAGTACCAAGAACATGACAATCTCGAACAGCAATATCGGAACAGGTGATGATTGCATTTCTATTGTAAGTGGATCCCAAAATGTGCAAGCCTCCAGTATCACCTGTGGACCTGGCCATGGAATCAGTATCGGTAGCTTGGGGAAAGATGGGTCTGAAGACTATGTTTCAAAAGTAACGGTGAACGGAGCTAAGTTTTCAGGAACCACGAACGGAGTCAGGATCAAGACATGGCAAGGAGGCTCAGGAATGGCAAGTAACATTGCATTTCAAAACATAGAAATGAAAAATGTGTATAACCCCATAATCATAGACCAAAACTACTGCGACACCAGTGACGGAAAATGCAAACAACAGAGCAAGGCAGTGAAGGTACGAAATGTGTTGTACCAAAACATCAGAGGGACAAGCGCTTCGCAAGATGCCATAACATTTGACTGCAGCAAGAGCATTCCATGCCAGGGAATTGTGCTGCAAAATGTTCAACTCAACAAAGGAGCAGAATGCAGCAATGTTAATCTGGCCTACAAAGGAAATGTCTCCCCTCGATGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

450

Amino Acids

48.33

Weight (kDa)

8.51

Isoelectric Point (pI)

35.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 113 - 437 2.7e-102 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 473
AccB1I GGYRCC 2 cut(s) 352, 367
AccI GTMKAC 1 cut(s) 477
AciI CCGC 1 cut(s) 612
AclI AACGTT 1 cut(s) 516
AclWI GGATC 4 cut(s) 509, 861, 874, 1022
AcoI YGGCCR 1 cut(s) 907
AcuI CTGAAG 2 cut(s) 214, 969
AfaI GTAC 5 cut(s) 170, 616, 799, 1167, 1181
AfeI AGCGCT 1 cut(s) 1204
AfiI CCNNNNNNNGG 2 cut(s) 911, 1253
AflII CTTAAG 1 cut(s) 674
AgsI TTSAA 6 cut(s) 536, 683, 731, 963, 1064, 1277
AhlI ACTAGT 1 cut(s) 764
AjiI CACGTC 1 cut(s) 703
AjnI CCWGG 3 cut(s) 541, 904, 1251
AjuI GAANNNNNNNTTGG 2 cut(s) 388, 420
AluBI AGCT 9 cut(s) 15, 78, 221, 242, 381, 427, 734, 930, 983
AluI AGCT 9 cut(s) 15, 78, 221, 242, 381, 427, 734, 930, 983
Alw21I GWGCWC 3 cut(s) 17, 223, 722
Alw26I GTCTC 2 cut(s) 755, 1336
Alw44I GTGCAC 1 cut(s) 718
AlwI GGATC 4 cut(s) 509, 861, 874, 1022
AlwNI CAGNNNCTG 1 cut(s) 752
Aor51HI AGCGCT 1 cut(s) 1204
AoxI GGCC 3 cut(s) 410, 907, 1314
ApaLI GTGCAC 1 cut(s) 718
ApeKI GCWGC 5 cut(s) 75, 293, 1233, 1264, 1299
ArsI GACNNNNNNTTYG 4 cut(s) 1189, 1221, 1314, 1346
AspLEI GCGC 3 cut(s) 278, 757, 1205
AspS9I GGNCC 3 cut(s) 410, 539, 902
AsuHPI GGTGA 4 cut(s) 476, 851, 886, 985
AsuII TTCGAA 1 cut(s) 711
AvaII GGWCC 2 cut(s) 539, 902
BaeGI GKGCMC 2 cut(s) 355, 722
BalI TGGCCA 1 cut(s) 909
BamHI GGATCC 1 cut(s) 866
BanI GGYRCC 2 cut(s) 352, 367
BanII GRGCYC 2 cut(s) 17, 223
BbsI GAAGAC 2 cut(s) 335, 957
Bbv12I GWGCWC 3 cut(s) 17, 223, 722
BbvI GCAGC 5 cut(s) 87, 280, 1245, 1251, 1311
BccI CCATC 2 cut(s) 557, 935
BceAI ACGGC 2 cut(s) 645, 796
BciT130I CCWGG 3 cut(s) 543, 906, 1253
BcoDI GTCTC 2 cut(s) 755, 1336
BcuI ACTAGT 1 cut(s) 764
BfaI CTAG 1 cut(s) 765
BfmI CTRYAG 2 cut(s) 478, 1231
BfoI RGCGCY 2 cut(s) 279, 1206
BfrI CTTAAG 1 cut(s) 674
BglII AGATCT 1 cut(s) 487
BisI GCNGC 5 cut(s) 76, 294, 1234, 1265, 1300
BlsI GCNGC 5 cut(s) 77, 295, 1235, 1266, 1301
Bme1390I CCNGG 3 cut(s) 543, 906, 1253
Bme18I GGWCC 2 cut(s) 539, 902
BmgBI CACGTC 1 cut(s) 703
BmgT120I GGNCC 3 cut(s) 410, 539, 902
BmiI GGNNCC 7 cut(s) 148, 354, 369, 448, 547, 868, 997
BmrFI CCNGG 3 cut(s) 543, 906, 1253
BmrI ACTGGG 2 cut(s) 144, 357
BmsI GCATC 5 cut(s) 186, 467, 676, 687, 1204
BmuI ACTGGG 2 cut(s) 144, 357
BpiI GAAGAC 2 cut(s) 335, 957
BplI GAGNNNNNCTC 2 cut(s) 210, 242
BpmI CTGGAG 1 cut(s) 871
Bpu10I CCTNAGC 1 cut(s) 1036
Bpu14I TTCGAA 1 cut(s) 711
BsaBI GATNNNNATC 2 cut(s) 120, 492
BsaJI CCNNGG 2 cut(s) 910, 1252
Bsc4I CCNNNNNNNGG 2 cut(s) 911, 1253
Bse1I ACTGG 6 cut(s) 139, 352, 503, 795, 888, 1125
Bse3DI GCAATG 3 cut(s) 20, 1053, 1309
Bse8I GATNNNNATC 2 cut(s) 120, 492
BseBI CCWGG 3 cut(s) 543, 906, 1253
BseDI CCNNGG 2 cut(s) 910, 1252
BseGI GGATG 1 cut(s) 160
BseJI GATNNNNATC 2 cut(s) 120, 492
BseLI CCNNNNNNNGG 2 cut(s) 911, 1253
BseMI GCAATG 3 cut(s) 20, 1053, 1309
BseMII CTCAG 2 cut(s) 437, 1050
BseNI ACTGG 6 cut(s) 139, 352, 503, 795, 888, 1125
BseSI GKGCMC 2 cut(s) 355, 722
BseXI GCAGC 5 cut(s) 87, 280, 1245, 1251, 1311
BshFI GGCC 3 cut(s) 412, 909, 1316
BshNI GGYRCC 2 cut(s) 352, 367
BsiHKAI GWGCWC 3 cut(s) 17, 223, 722
BsiSI CCGG 1 cut(s) 758
BslFI GGGAC 2 cut(s) 753, 1210
BslI CCNNNNNNNGG 2 cut(s) 911, 1253
BsmAI GTCTC 2 cut(s) 755, 1336
BsmFI GGGAC 2 cut(s) 753, 1210
BsmI GAATGC 3 cut(s) 783, 1242, 1301
BsnI GGCC 3 cut(s) 412, 909, 1316
Bsp119I TTCGAA 1 cut(s) 711
Bsp1286I GDGCHC 4 cut(s) 17, 223, 355, 722
Bsp143I GATC 4 cut(s) 487, 501, 866, 1014
Bsp19I CCATGG 1 cut(s) 910
BspACI CCGC 1 cut(s) 612
BspANI GGCC 3 cut(s) 412, 909, 1316
BspCNI CTCAG 2 cut(s) 436, 1049
BspLI GGNNCC 7 cut(s) 148, 354, 369, 448, 547, 868, 997
BspMAI CTGCAG 1 cut(s) 1235
BspPI GGATC 4 cut(s) 509, 861, 874, 1022
BspQI GCTCTTC 3 cut(s) 22, 233, 371
BspT104I TTCGAA 1 cut(s) 711
BspT107I GGYRCC 2 cut(s) 352, 367
BspTI CTTAAG 1 cut(s) 674
BsrDI GCAATG 3 cut(s) 20, 1053, 1309
BsrI ACTGG 6 cut(s) 139, 352, 503, 795, 888, 1125
BssECI CCNNGG 2 cut(s) 910, 1252
BssMI GATC 4 cut(s) 487, 501, 866, 1014
BssT1I CCWWGG 1 cut(s) 910
Bst2UI CCWGG 3 cut(s) 543, 906, 1253
Bst4CI ACNGT 3 cut(s) 563, 748, 973
Bst6I CTCTTC 3 cut(s) 22, 233, 371
BstAFI CTTAAG 1 cut(s) 674
BstAPI GCANNNNNTGC 1 cut(s) 1248
BstBI TTCGAA 1 cut(s) 711
BstC8I GCNNGC 2 cut(s) 621, 882
BstDEI CTNAG 4 cut(s) 423, 603, 984, 1036
BstDSI CCRYGG 1 cut(s) 910
BstF5I GGATG 1 cut(s) 160
BstH2I RGCGCY 2 cut(s) 279, 1206
BstHHI GCGC 3 cut(s) 278, 757, 1205
BstKTI GATC 4 cut(s) 490, 504, 869, 1017
BstMAI GTCTC 2 cut(s) 755, 1336
BstMBI GATC 4 cut(s) 487, 501, 866, 1014
BstMWI GCNNNNNNNGC 5 cut(s) 72, 433, 629, 1239, 1248
BstNI CCWGG 3 cut(s) 543, 906, 1253
BstSCI CCNGG 3 cut(s) 541, 904, 1251
BstSFI CTRYAG 2 cut(s) 478, 1231
BstSLI GKGCMC 2 cut(s) 355, 722
BstV1I GCAGC 5 cut(s) 87, 280, 1245, 1251, 1311
BstV2I GAAGAC 2 cut(s) 335, 957
BstX2I RGATCY 2 cut(s) 487, 866
BstYI RGATCY 2 cut(s) 487, 866
BsuRI GGCC 3 cut(s) 412, 909, 1316
BtgI CCRYGG 1 cut(s) 910
BtrI CACGTC 1 cut(s) 703
BtsCI GGATG 1 cut(s) 160
BtsI GCAGTG 1 cut(s) 1164
BtsIMutI CAGTG 5 cut(s) 345, 496, 774, 1132, 1164
Cac8I GCNNGC 2 cut(s) 621, 882
CaiI CAGNNNCTG 1 cut(s) 752
CfoI GCGC 3 cut(s) 278, 757, 1205
Cfr13I GGNCC 3 cut(s) 410, 539, 902
CseI GACGC 1 cut(s) 250
Csp6I GTAC 5 cut(s) 169, 615, 798, 1166, 1180
CspCI CAANNNNNGTGG 2 cut(s) 554, 589
CviAII CATG 9 cut(s) 63, 172, 324, 414, 505, 808, 911, 1023, 1248
CviQI GTAC 5 cut(s) 169, 615, 798, 1166, 1180
DdeI CTNAG 4 cut(s) 423, 603, 984, 1036
DpnI GATC 4 cut(s) 489, 503, 868, 1016
DpnII GATC 4 cut(s) 487, 501, 866, 1014
DrdI GACNNNNNNGTC 1 cut(s) 473
DseDI GACNNNNNNGTC 1 cut(s) 473
EaeI YGGCCR 1 cut(s) 907
Eam1104I CTCTTC 3 cut(s) 22, 233, 371
EarI CTCTTC 3 cut(s) 22, 233, 371
Ecl136II GAGCTC 2 cut(s) 15, 221
Eco130I CCWWGG 1 cut(s) 910
Eco24I GRGCYC 2 cut(s) 17, 223
Eco47I GGWCC 2 cut(s) 539, 902
Eco47III AGCGCT 1 cut(s) 1204
Eco53kI GAGCTC 2 cut(s) 15, 221
Eco57I CTGAAG 2 cut(s) 214, 969
EcoICRI GAGCTC 2 cut(s) 15, 221
EcoO109I RGGNCCY 1 cut(s) 539
EcoRII CCWGG 3 cut(s) 541, 904, 1251
EcoT14I CCWWGG 1 cut(s) 910
EcoT38I GRGCYC 2 cut(s) 17, 223
ErhI CCWWGG 1 cut(s) 910
FaeI CATG 9 cut(s) 66, 175, 327, 417, 508, 811, 914, 1026, 1251
FaqI GGGAC 2 cut(s) 753, 1210
FatI CATG 9 cut(s) 62, 171, 323, 413, 504, 807, 910, 1022, 1247
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 5 cut(s) 76, 294, 1234, 1265, 1300
FokI GGATG 1 cut(s) 147
FriOI GRGCYC 2 cut(s) 17, 223
Fsp4HI GCNGC 5 cut(s) 76, 294, 1234, 1265, 1300
FspBI CTAG 1 cut(s) 765
GlaI GCGC 3 cut(s) 277, 756, 1204
GluI GCNGC 5 cut(s) 76, 294, 1234, 1265, 1300
GsuI CTGGAG 1 cut(s) 871
HaeII RGCGCY 2 cut(s) 279, 1206
HaeIII GGCC 3 cut(s) 412, 909, 1316
HapII CCGG 1 cut(s) 758
HgaI GACGC 1 cut(s) 250
HhaI GCGC 3 cut(s) 278, 757, 1205
Hin1II CATG 9 cut(s) 66, 175, 327, 417, 508, 811, 914, 1026, 1251
Hin6I GCGC 3 cut(s) 276, 755, 1203
HinP1I GCGC 3 cut(s) 276, 755, 1203
HindIII AAGCTT 1 cut(s) 732
HinfI GANTC 5 cut(s) 187, 203, 670, 915, 1008
HpaII CCGG 1 cut(s) 758
HphI GGTGA 4 cut(s) 476, 851, 886, 985
Hpy166II GTNNAC 4 cut(s) 478, 720, 902, 976
Hpy188I TCNGA 6 cut(s) 151, 202, 218, 833, 949, 1193
Hpy188III TCNNGA 8 cut(s) 125, 664, 683, 817, 993, 1012, 1018, 1038
Hpy8I GTNNAC 4 cut(s) 478, 720, 902, 976
Hpy99I CGWCG 1 cut(s) 266
HpyAV CCTTC 4 cut(s) 165, 174, 707, 1156
HpyCH4III ACNGT 3 cut(s) 563, 748, 973
HpyCH4IV ACGT 2 cut(s) 516, 702
HpyF10VI GCNNNNNNNGC 5 cut(s) 72, 433, 629, 1239, 1248
HpyF3I CTNAG 4 cut(s) 423, 603, 984, 1036
HpySE526I ACGT 2 cut(s) 516, 702
Hsp92II CATG 9 cut(s) 66, 175, 327, 417, 508, 811, 914, 1026, 1251
HspAI GCGC 3 cut(s) 276, 755, 1203
Kzo9I GATC 4 cut(s) 487, 501, 866, 1014
LguI GCTCTTC 3 cut(s) 22, 233, 371
LmnI GCTCC 2 cut(s) 980, 1289
Lsp1109I GCAGC 5 cut(s) 87, 280, 1245, 1251, 1311
LweI GCATC 5 cut(s) 186, 467, 676, 687, 1204
MaeI CTAG 1 cut(s) 765
MaeII ACGT 2 cut(s) 516, 702
MaeIII GTNAC 6 cut(s) 464, 634, 748, 967, 1049, 1127
MalI GATC 4 cut(s) 489, 503, 868, 1016
MboI GATC 4 cut(s) 487, 501, 866, 1014
MboII GAAGA 9 cut(s) 9, 29, 51, 140, 220, 340, 388, 679, 962
MflI RGATCY 2 cut(s) 487, 866
MhlI GDGCHC 4 cut(s) 17, 223, 355, 722
MlsI TGGCCA 1 cut(s) 909
MluCI AATT 1 cut(s) 1257
MluNI TGGCCA 1 cut(s) 909
MlyI GAGTC 1 cut(s) 1017
MnlI CCTC 4 cut(s) 895, 1025, 1187, 1347
Mox20I TGGCCA 1 cut(s) 909
MscI TGGCCA 1 cut(s) 909
MseI TTAA 5 cut(s) 249, 390, 519, 675, 1308
MslI CAYNNNNRTG 2 cut(s) 105, 1073
Msp20I TGGCCA 1 cut(s) 909
MspCI CTTAAG 1 cut(s) 674
MspI CCGG 1 cut(s) 758
MspR9I CCNGG 3 cut(s) 543, 906, 1253
Mva1269I GAATGC 3 cut(s) 783, 1242, 1301
MvaI CCWGG 3 cut(s) 543, 906, 1253
MwoI GCNNNNNNNGC 5 cut(s) 72, 433, 629, 1239, 1248
NcoI CCATGG 1 cut(s) 910
NdeII GATC 4 cut(s) 487, 501, 866, 1014
NlaIII CATG 9 cut(s) 66, 175, 327, 417, 508, 811, 914, 1026, 1251
NlaIV GGNNCC 7 cut(s) 148, 354, 369, 448, 547, 868, 997
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 3 cut(s) 464, 634, 1127
NspV TTCGAA 1 cut(s) 711
PciSI GCTCTTC 3 cut(s) 22, 233, 371
PcsI WCGNNNNNNNCGW 1 cut(s) 708
PctI GAATGC 3 cut(s) 783, 1242, 1301
PfeI GAWTC 4 cut(s) 187, 203, 670, 915
PfoI TCCNGGA 1 cut(s) 541
PkrI GCNGC 5 cut(s) 77, 295, 1235, 1266, 1301
PleI GAGTC 1 cut(s) 1016
PpsI GAGTC 1 cut(s) 1016
PpuMI RGGWCCY 1 cut(s) 539
Psp124BI GAGCTC 2 cut(s) 17, 223
Psp1406I AACGTT 1 cut(s) 516
Psp5II RGGWCCY 1 cut(s) 539
Psp6I CCWGG 3 cut(s) 541, 904, 1251
PspGI CCWGG 3 cut(s) 541, 904, 1251
PspN4I GGNNCC 7 cut(s) 148, 354, 369, 448, 547, 868, 997
PspPI GGNCC 3 cut(s) 410, 539, 902
PspPPI RGGWCCY 1 cut(s) 539
PstI CTGCAG 1 cut(s) 1235
PstNI CAGNNNCTG 1 cut(s) 752
PsuI RGATCY 2 cut(s) 487, 866
RsaI GTAC 5 cut(s) 170, 616, 799, 1167, 1181
RsaNI GTAC 5 cut(s) 169, 615, 798, 1166, 1180
RseI CAYNNNNRTG 2 cut(s) 105, 1073
SacI GAGCTC 2 cut(s) 17, 223
SapI GCTCTTC 3 cut(s) 22, 233, 371
SaqAI TTAA 5 cut(s) 249, 390, 519, 675, 1308
SatI GCNGC 5 cut(s) 76, 294, 1234, 1265, 1300
Sau3AI GATC 4 cut(s) 487, 501, 866, 1014
Sau96I GGNCC 3 cut(s) 410, 539, 902
SchI GAGTC 1 cut(s) 1017
ScrFI CCNGG 3 cut(s) 543, 906, 1253
SduI GDGCHC 4 cut(s) 17, 223, 355, 722
SfaNI GCATC 5 cut(s) 186, 467, 676, 687, 1204
SfcI CTRYAG 2 cut(s) 478, 1231
SfuI TTCGAA 1 cut(s) 711
SinI GGWCC 2 cut(s) 539, 902
SmiMI CAYNNNNRTG 2 cut(s) 105, 1073
SmlI CTYRAG 1 cut(s) 674
SmoI CTYRAG 1 cut(s) 674
SpeI ACTAGT 1 cut(s) 764
Sse9I AATT 1 cut(s) 1257
SsiI CCGC 1 cut(s) 612
SspMI CTAG 1 cut(s) 765
SstI GAGCTC 2 cut(s) 17, 223
StyD4I CCNGG 3 cut(s) 541, 904, 1251
StyI CCWWGG 1 cut(s) 910
TaaI ACNGT 3 cut(s) 563, 748, 973
TaiI ACGT 2 cut(s) 519, 705
TaqI TCGA 6 cut(s) 111, 206, 264, 711, 818, 1339
TasI AATT 1 cut(s) 1257
TfiI GAWTC 4 cut(s) 187, 203, 670, 915
Tru1I TTAA 5 cut(s) 249, 390, 519, 675, 1308
Tru9I TTAA 5 cut(s) 249, 390, 519, 675, 1308
TscAI CASTG 5 cut(s) 352, 503, 781, 1132, 1164
TseFI GTSAC 3 cut(s) 464, 634, 1127
TseI GCWGC 5 cut(s) 75, 293, 1233, 1264, 1299
Tsp45I GTSAC 3 cut(s) 464, 634, 1127
TspDTI ATGAA 4 cut(s) 9, 51, 776, 1091
TspGWI ACGGA 4 cut(s) 235, 993, 1020, 1146
TspRI CASTG 5 cut(s) 352, 503, 781, 1132, 1164
Vha464I CTTAAG 1 cut(s) 674
VneI GTGCAC 1 cut(s) 718
VpaK11BI GGWCC 2 cut(s) 539, 902
XmiI GTMKAC 1 cut(s) 477
XspI CTAG 1 cut(s) 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.