Rh6CG306100

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0003318
Physical Location & Seq
Reverse (-)
48663354 .. 48665580
2227 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG306100.1

Sequence Viewer

Length: 921 bp
ATGCAGATATATGGAACCATAGAAGCATCTGGTGACAGGTCAGTCTACAGCAAAGATCTCAATCACTGGATCATGTTTGACAACGTTCGAAATCTGTTAGTTCAGGGTCCTGGAACCATCAATGGCAACGGTCAAATCTGGTGGCAAAACTCTTGCAAAAGAAAACAAACCAAGCCCTGCGGGACGCAAGCACCTACGGCTGTGACCTTCTACAAATGTAATAACTTGGTGGTGAAGGATCTGAAGTTCCAAGACGCACAGCAAATGCACGTCAGTTTCGAAGAGTGCACTAATGTTCAAGCTTTGTATCTAACAGTAACTGCGCCGGAGACTAGTCCCAACACTGACGGCATTCATGTTGCCAATACCCAGAACATCACAATCTCGAACAGTAATATCGGAACAGGTGATGATTGCATTTCAATTGTGAGTGGATCCCAAAATGTGCAAGCCTCCAGTATTACCTGTGGACCTGGCCATGGAATCAGTATCGGTAGCTTGGGGAAAGGAGGGTCTGAAGACCATGTTTCGCAAGTAACAGTGAACGGAGCTAAGATTTCAGGAACCACGAATGGAATCAGGATCAAAACATGGCAAGGAGGCTCAGGAATGGCAAGCGACATTGCTTTTCAAAACATAGAAATGAATGATGTGACCAACCCCATAATCATAGACCAAAACTACTGTGACACCAGTGACGAAGGACAATGCAAACAACAGAGCAAGGCAGTGAAGGTACAAAATGTGTTGTACCAAAACATCAGAGGGACAAGCACTTCAAAAGATGCCATAACATTTGATTGCAGCAAGAGCATTCCATGTCAGGGAATTGTGCTGCAAAATGTTCAACTCGACAAAGGAGCAGAATGCAGCAATGTTAATCTGGCTTACAAAGGAAATGTCTCCCCTCGATGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

306

Amino Acids

32.87

Weight (kDa)

6.2

Isoelectric Point (pI)

30.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 2 - 294 1.2e-93 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 41
AccI GTMKAC 1 cut(s) 45
AciI CCGC 1 cut(s) 180
AclI AACGTT 1 cut(s) 84
AclWI GGATC 5 cut(s) 77, 246, 429, 442, 590
AcoI YGGCCR 1 cut(s) 475
AcuI CTGAAG 2 cut(s) 263, 537
AfaI GTAC 2 cut(s) 738, 752
AfiI CCNNNNNNNGG 2 cut(s) 479, 824
AgsI TTSAA 5 cut(s) 299, 423, 632, 780, 848
AhlI ACTAGT 1 cut(s) 332
AjiI CACGTC 1 cut(s) 271
AjnI CCWGG 2 cut(s) 109, 472
AloI GAACNNNNNNTCC 2 cut(s) 230, 262
AluBI AGCT 3 cut(s) 302, 498, 551
AluI AGCT 3 cut(s) 302, 498, 551
Alw21I GWGCWC 1 cut(s) 290
Alw26I GTCTC 2 cut(s) 323, 907
Alw44I GTGCAC 1 cut(s) 286
AlwI GGATC 5 cut(s) 77, 246, 429, 442, 590
AlwNI CAGNNNCTG 1 cut(s) 320
AoxI GGCC 1 cut(s) 475
ApaLI GTGCAC 1 cut(s) 286
ApeKI GCWGC 3 cut(s) 804, 835, 870
ArsI GACNNNNNNTTYG 2 cut(s) 885, 917
AspLEI GCGC 1 cut(s) 325
AspS9I GGNCC 2 cut(s) 107, 470
AsuHPI GGTGA 3 cut(s) 44, 244, 419
AsuII TTCGAA 2 cut(s) 88, 279
AvaII GGWCC 2 cut(s) 107, 470
BaeGI GKGCMC 1 cut(s) 290
BalI TGGCCA 1 cut(s) 477
BamHI GGATCC 1 cut(s) 434
BbsI GAAGAC 1 cut(s) 525
Bbv12I GWGCWC 1 cut(s) 290
BbvI GCAGC 3 cut(s) 816, 822, 882
BccI CCATC 1 cut(s) 125
BceAI ACGGC 2 cut(s) 213, 364
BciT130I CCWGG 2 cut(s) 111, 474
BcoDI GTCTC 2 cut(s) 323, 907
BcuI ACTAGT 1 cut(s) 332
BfaI CTAG 1 cut(s) 333
BfmI CTRYAG 1 cut(s) 46
BglII AGATCT 1 cut(s) 55
BisI GCNGC 3 cut(s) 805, 836, 871
BlsI GCNGC 3 cut(s) 806, 837, 872
Bme1390I CCNGG 2 cut(s) 111, 474
Bme18I GGWCC 2 cut(s) 107, 470
BmgBI CACGTC 1 cut(s) 271
BmgT120I GGNCC 2 cut(s) 107, 470
BmiI GGNNCC 5 cut(s) 16, 108, 115, 436, 565
BmrFI CCNGG 2 cut(s) 111, 474
BmsI GCATC 2 cut(s) 35, 775
BpiI GAAGAC 1 cut(s) 525
BpmI CTGGAG 1 cut(s) 439
Bpu10I CCTNAGC 1 cut(s) 604
Bpu14I TTCGAA 2 cut(s) 88, 279
BsaBI GATNNNNATC 1 cut(s) 60
BsaJI CCNNGG 1 cut(s) 478
Bsc4I CCNNNNNNNGG 2 cut(s) 479, 824
Bse1I ACTGG 3 cut(s) 71, 456, 693
Bse3DI GCAATG 2 cut(s) 621, 880
Bse8I GATNNNNATC 1 cut(s) 60
BseBI CCWGG 2 cut(s) 111, 474
BseDI CCNNGG 1 cut(s) 478
BseJI GATNNNNATC 1 cut(s) 60
BseLI CCNNNNNNNGG 2 cut(s) 479, 824
BseMI GCAATG 2 cut(s) 621, 880
BseMII CTCAG 1 cut(s) 618
BseNI ACTGG 3 cut(s) 71, 456, 693
BseSI GKGCMC 1 cut(s) 290
BseXI GCAGC 3 cut(s) 816, 822, 882
BshFI GGCC 1 cut(s) 477
BsiHKAI GWGCWC 1 cut(s) 290
BsiSI CCGG 1 cut(s) 326
BslFI GGGAC 3 cut(s) 196, 321, 781
BslI CCNNNNNNNGG 2 cut(s) 479, 824
BsmAI GTCTC 2 cut(s) 323, 907
BsmFI GGGAC 3 cut(s) 196, 321, 781
BsmI GAATGC 3 cut(s) 351, 813, 872
BsnI GGCC 1 cut(s) 477
Bsp119I TTCGAA 2 cut(s) 88, 279
Bsp1286I GDGCHC 1 cut(s) 290
Bsp143I GATC 5 cut(s) 55, 69, 238, 434, 582
Bsp19I CCATGG 1 cut(s) 478
BspACI CCGC 1 cut(s) 180
BspANI GGCC 1 cut(s) 477
BspCNI CTCAG 1 cut(s) 617
BspLI GGNNCC 5 cut(s) 16, 108, 115, 436, 565
BspPI GGATC 5 cut(s) 77, 246, 429, 442, 590
BspT104I TTCGAA 2 cut(s) 88, 279
BsrDI GCAATG 2 cut(s) 621, 880
BsrI ACTGG 3 cut(s) 71, 456, 693
BssECI CCNNGG 1 cut(s) 478
BssMI GATC 5 cut(s) 55, 69, 238, 434, 582
BssT1I CCWWGG 1 cut(s) 478
Bst2UI CCWGG 2 cut(s) 111, 474
Bst4CI ACNGT 5 cut(s) 131, 316, 392, 541, 686
Bst6I CTCTTC 1 cut(s) 276
BstBI TTCGAA 2 cut(s) 88, 279
BstC8I GCNNGC 3 cut(s) 189, 450, 616
BstDEI CTNAG 2 cut(s) 552, 604
BstDSI CCRYGG 1 cut(s) 478
BstHHI GCGC 1 cut(s) 325
BstKTI GATC 5 cut(s) 58, 72, 241, 437, 585
BstMAI GTCTC 2 cut(s) 323, 907
BstMBI GATC 5 cut(s) 55, 69, 238, 434, 582
BstMWI GCNNNNNNNGC 2 cut(s) 197, 810
BstNI CCWGG 2 cut(s) 111, 474
BstSCI CCNGG 2 cut(s) 109, 472
BstSFI CTRYAG 1 cut(s) 46
BstSLI GKGCMC 1 cut(s) 290
BstV1I GCAGC 3 cut(s) 816, 822, 882
BstV2I GAAGAC 1 cut(s) 525
BstX2I RGATCY 3 cut(s) 55, 238, 434
BstYI RGATCY 3 cut(s) 55, 238, 434
BsuRI GGCC 1 cut(s) 477
BtgI CCRYGG 1 cut(s) 478
BtrI CACGTC 1 cut(s) 271
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 5 cut(s) 64, 342, 546, 700, 735
Cac8I GCNNGC 3 cut(s) 189, 450, 616
CaiI CAGNNNCTG 1 cut(s) 320
CfoI GCGC 1 cut(s) 325
Cfr13I GGNCC 2 cut(s) 107, 470
CseI GACGC 2 cut(s) 193, 263
Csp6I GTAC 2 cut(s) 737, 751
CspCI CAANNNNNGTGG 2 cut(s) 122, 157
CviAII CATG 6 cut(s) 73, 356, 479, 524, 591, 819
CviJI RGCY 9 cut(s) 175, 200, 302, 452, 477, 498, 551, 603, 887
CviKI_1 RGCY 9 cut(s) 175, 200, 302, 452, 477, 498, 551, 603, 887
CviQI GTAC 2 cut(s) 737, 751
DdeI CTNAG 2 cut(s) 552, 604
DpnI GATC 5 cut(s) 57, 71, 240, 436, 584
DpnII GATC 5 cut(s) 55, 69, 238, 434, 582
DrdI GACNNNNNNGTC 1 cut(s) 41
DseDI GACNNNNNNGTC 1 cut(s) 41
EaeI YGGCCR 1 cut(s) 475
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
Eco130I CCWWGG 1 cut(s) 478
Eco47I GGWCC 2 cut(s) 107, 470
Eco57I CTGAAG 2 cut(s) 263, 537
EcoO109I RGGNCCY 1 cut(s) 107
EcoRII CCWGG 2 cut(s) 109, 472
EcoT14I CCWWGG 1 cut(s) 478
ErhI CCWWGG 1 cut(s) 478
FaeI CATG 6 cut(s) 76, 359, 482, 527, 594, 822
FaqI GGGAC 3 cut(s) 196, 321, 781
FatI CATG 6 cut(s) 72, 355, 478, 523, 590, 818
FauI CCCGC 1 cut(s) 173
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 3 cut(s) 805, 836, 871
Fsp4HI GCNGC 3 cut(s) 805, 836, 871
FspBI CTAG 1 cut(s) 333
GlaI GCGC 1 cut(s) 324
GluI GCNGC 3 cut(s) 805, 836, 871
GsuI CTGGAG 1 cut(s) 439
HaeIII GGCC 1 cut(s) 477
HapII CCGG 1 cut(s) 326
HgaI GACGC 2 cut(s) 193, 263
HhaI GCGC 1 cut(s) 325
Hin1II CATG 6 cut(s) 76, 359, 482, 527, 594, 822
Hin6I GCGC 1 cut(s) 323
HinP1I GCGC 1 cut(s) 323
HindIII AAGCTT 1 cut(s) 300
HinfI GANTC 2 cut(s) 483, 576
HpaII CCGG 1 cut(s) 326
HphI GGTGA 3 cut(s) 44, 244, 419
Hpy166II GTNNAC 4 cut(s) 46, 288, 470, 544
Hpy188I TCNGA 4 cut(s) 243, 401, 517, 764
Hpy188III TCNNGA 4 cut(s) 385, 561, 580, 606
Hpy8I GTNNAC 4 cut(s) 46, 288, 470, 544
HpyAV CCTTC 4 cut(s) 217, 229, 695, 727
HpyCH4III ACNGT 5 cut(s) 131, 316, 392, 541, 686
HpyCH4IV ACGT 2 cut(s) 84, 270
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 810
HpyF3I CTNAG 2 cut(s) 552, 604
HpySE526I ACGT 2 cut(s) 84, 270
Hsp92II CATG 6 cut(s) 76, 359, 482, 527, 594, 822
HspAI GCGC 1 cut(s) 323
Kzo9I GATC 5 cut(s) 55, 69, 238, 434, 582
LmnI GCTCC 2 cut(s) 548, 860
Lsp1109I GCAGC 3 cut(s) 816, 822, 882
LweI GCATC 2 cut(s) 35, 775
MaeI CTAG 1 cut(s) 333
MaeII ACGT 2 cut(s) 84, 270
MaeIII GTNAC 7 cut(s) 32, 202, 316, 535, 652, 686, 695
MalI GATC 5 cut(s) 57, 71, 240, 436, 584
MboI GATC 5 cut(s) 55, 69, 238, 434, 582
MboII GAAGA 2 cut(s) 293, 530
MfeI CAATTG 1 cut(s) 423
MflI RGATCY 3 cut(s) 55, 238, 434
MhlI GDGCHC 1 cut(s) 290
MlsI TGGCCA 1 cut(s) 477
MluCI AATT 2 cut(s) 423, 828
MluNI TGGCCA 1 cut(s) 477
MnlI CCTC 5 cut(s) 463, 503, 593, 758, 918
Mox20I TGGCCA 1 cut(s) 477
MscI TGGCCA 1 cut(s) 477
MseI TTAA 2 cut(s) 879, 919
MslI CAYNNNNRTG 1 cut(s) 641
Msp20I TGGCCA 1 cut(s) 477
MspI CCGG 1 cut(s) 326
MspR9I CCNGG 2 cut(s) 111, 474
MunI CAATTG 1 cut(s) 423
Mva1269I GAATGC 3 cut(s) 351, 813, 872
MvaI CCWGG 2 cut(s) 111, 474
MwoI GCNNNNNNNGC 2 cut(s) 197, 810
NcoI CCATGG 1 cut(s) 478
NdeII GATC 5 cut(s) 55, 69, 238, 434, 582
NlaIII CATG 6 cut(s) 76, 359, 482, 527, 594, 822
NlaIV GGNNCC 5 cut(s) 16, 108, 115, 436, 565
NmuCI GTSAC 5 cut(s) 32, 202, 652, 686, 695
NspV TTCGAA 2 cut(s) 88, 279
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 3 cut(s) 351, 813, 872
PfeI GAWTC 2 cut(s) 483, 576
PfoI TCCNGGA 1 cut(s) 109
PkrI GCNGC 3 cut(s) 806, 837, 872
PpuMI RGGWCCY 1 cut(s) 107
Psp1406I AACGTT 1 cut(s) 84
Psp5II RGGWCCY 1 cut(s) 107
Psp6I CCWGG 2 cut(s) 109, 472
PspGI CCWGG 2 cut(s) 109, 472
PspN4I GGNNCC 5 cut(s) 16, 108, 115, 436, 565
PspPI GGNCC 2 cut(s) 107, 470
PspPPI RGGWCCY 1 cut(s) 107
PstNI CAGNNNCTG 1 cut(s) 320
PsuI RGATCY 3 cut(s) 55, 238, 434
RsaI GTAC 2 cut(s) 738, 752
RsaNI GTAC 2 cut(s) 737, 751
RseI CAYNNNNRTG 1 cut(s) 641
SaqAI TTAA 2 cut(s) 879, 919
SatI GCNGC 3 cut(s) 805, 836, 871
Sau3AI GATC 5 cut(s) 55, 69, 238, 434, 582
Sau96I GGNCC 2 cut(s) 107, 470
ScrFI CCNGG 2 cut(s) 111, 474
SduI GDGCHC 1 cut(s) 290
SfaNI GCATC 2 cut(s) 35, 775
SfcI CTRYAG 1 cut(s) 46
SfuI TTCGAA 2 cut(s) 88, 279
SinI GGWCC 2 cut(s) 107, 470
SmiMI CAYNNNNRTG 1 cut(s) 641
SpeI ACTAGT 1 cut(s) 332
Sse9I AATT 2 cut(s) 423, 828
SsiI CCGC 1 cut(s) 180
SspMI CTAG 1 cut(s) 333
StyD4I CCNGG 2 cut(s) 109, 472
StyI CCWWGG 1 cut(s) 478
TaaI ACNGT 5 cut(s) 131, 316, 392, 541, 686
TaiI ACGT 2 cut(s) 87, 273
TaqI TCGA 5 cut(s) 88, 279, 386, 852, 910
TasI AATT 2 cut(s) 423, 828
TfiI GAWTC 2 cut(s) 483, 576
Tru1I TTAA 2 cut(s) 879, 919
Tru9I TTAA 2 cut(s) 879, 919
TscAI CASTG 5 cut(s) 71, 349, 546, 700, 735
TseFI GTSAC 5 cut(s) 32, 202, 652, 686, 695
TseI GCWGC 3 cut(s) 804, 835, 870
Tsp45I GTSAC 5 cut(s) 32, 202, 652, 686, 695
TspDTI ATGAA 2 cut(s) 344, 659
TspGWI ACGGA 1 cut(s) 561
TspRI CASTG 5 cut(s) 71, 349, 546, 700, 735
VneI GTGCAC 1 cut(s) 286
VpaK11BI GGWCC 2 cut(s) 107, 470
XmiI GTMKAC 1 cut(s) 45
XspI CTAG 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.