Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003318

Physical Location & Seq

Genomic Coordinates Forward (+)

32069291 .. 32071704

Gene Structure 2414 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1192600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1353 bp

ATGGCTTTACAAACACATTTGTTATGGTCATTTGTTGTTCTTTTTGTTGTTTCCTTTGGTACAACTTCATGTTATGGTACTAGTTTCCAGGAGGTCAACTCTCTTCATAGTTACGTTGACCATGTTGATAAAGAGTCTGGCTACAATTCTAGGGCTTATCCTTCATACATGGACACTATTGAAGGTTTCAAGTTCATGGAATTGATCAGGCCAAGACCTCAGCTCTTCAGTTCAAGGAAGCTCAACAAAATCACCAGTGGCATAGCGACATCATCAGCTCCGTCCAAAACGATTAGCGTTGACGATTTTGGAGCTAAAGGGAATGGTGCTGATGACACACAGGCATTTGTGAAGGCATGGAAGGCAGCTTGTTCTTCCAGTGGAGCAATAGTTCTTGTGGTGCCACAGAAAAGCTATCTTGTTGGGCCAATTGAATTCTCAGGCCCCTGCAAATCTCGACTTACAATGCAGATTTATGGAACCATAGAAGCATCAGAAAACCGATCAATCTACAAAGACCTAGACCACTGGCTCATGTTTGACAACGTCCAAAACGTACTAGTTGTTGGTCCTGGAACCATCAATGGCAATGGAAACATATGGTGGAAAAACTCATGCAAAAGAAAGCCTCAGGCTGTGACCTTCAACAGGTGCAATAACTTGGTGGTGAAGAATCTGAAGATCCAAGACGCTCAACAAATGCATGTTAGATTCCAAAACTGCTTCAATGTTCAAGCTTCCCGTCTCACAGTAACTGCACCTGAGGATAGCCCCAATACTGATGGAATTCATGTTACAAATACCCAGAACATCACTATCTCGAGCTCGGTTATAGGAACAGGTGATGACTGTATTTCTATTGTAAGTGGGTCTCAAAGAGTTCAAGCCAGAGACATAACTTGTGGGCCAGGCCATGGAATCAGTATTGGTAGCTTGGGAAAAGACGGCTCAATAGACCATGTTTCAGGAGTATTTGTGAATGGAGCTAAACTTTCAGGAACCGCCAATGGACTCAGGATCAAGACATGGCAGGGAGGCTCAGGCAGTGCAACCAACATTGTTTTCCAGAATGTGCAAATGAATGATGTCACCAACCCCATCATAATCGACCAGAACTACTGTGACCACATAACCAAAGACTGCAAACAACAGAAATCGGCGGTTCAAGTGAAAAATGTGTTGTACCAAAACATTAGAGGAACAGGTGTTATCGGCGACGCGATAACGTTTAACTGTAGCCAAAGTGTTCCTTGTCAGGGGATTGTGCTGCAAAATATTCAACTGCAGAATGGAAGAGCTGAATGCAACGATGTTCAGCCTGCTTACAAAGGAGTTGTCTCTCCAAGATGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

Glyco_hydro_28

Protein Analysis

451

Amino Acids

48.9

Weight (kDa)

8.67

Isoelectric Point (pI)

33.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pect-lyase_RHGA_epim	PF12708	98 - 308	2.2e-06	Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28	PF00295	124 - 438	6.3e-97	Glycosyl hydrolases family 28

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003318)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41850
fragaria_vesca	FvH4_2g20930 FvH4_2g20930 FvH4_6g23580
malus_domestica	MD05G1192600.v1.1 MD10G1179100.v1.1 MD12G1077400.v1.1 MD12G1077600.v1.1
prunus_persica	Prupe.7G063900_v2.0.a1 Prupe.8G218900_v2.0.a1
pyrus_communis	pycom05g17960 pycom10g15530 pycom12g06230
rosa_chinensis	RchiOBHm_Chr3g0481691 RchiOBHm_Chr6g0287311
rosa_laevigata	RLG00000012502 RLG00000023383
rosa_multiflora	Rmu_sc0007029.1_g000032 Rmu_ssc0000267.1_g000001
rosa_roxburghii	Rroxscaffold_6G00399870 Rroxscaffold_6G00400230 Rroxscaffold_7G00181550
rosa_rugosa	Rorug03G0193400 Rorug06G0186800 Rorug06G0186900
rosa_samantha	Rh3AG244500 Rh3BG279600 Rh3CG274700 Rh3DG271500 Rh6AG298300 Rh6BG303700 Rh6CG306100 Rh6CG312000 Rh6DG295600
rosa_wichuraiana	Rw3G021820 Rw6G025780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	400
AccB7I	CCANNNNNTGG	1 cut(s)	914
AccII	CGCG	1 cut(s)	1220
AciI	CCGC	2 cut(s)	1002, 1160
AclI	AACGTT	1 cut(s)	1226
AclWI	GGATC	2 cut(s)	676, 1025
AcsI	RAATTY	2 cut(s)	434, 786
AcuI	CTGAAG	2 cut(s)	211, 698
AfaI	GTAC	4 cut(s)	61, 79, 558, 1184
AfiI	CCNNNNNNNGG	3 cut(s)	648, 914, 1256
AhlI	ACTAGT	2 cut(s)	80, 559
AjnI	CCWGG	3 cut(s)	87, 571, 907
AjuI	GAANNNNNNNTTGG	2 cut(s)	1046, 1078
AloI	GAACNNNNNNTCC	2 cut(s)	375, 407
Alw21I	GWGCWC	1 cut(s)	827
Alw26I	GTCTC	4 cut(s)	749, 876, 885, 1342
AlwI	GGATC	2 cut(s)	676, 1025
AlwNI	CAGNNNCTG	1 cut(s)	755
Ama87I	CYCGRG	1 cut(s)	820
AoxI	GGCC	5 cut(s)	209, 425, 442, 905, 910
ApeKI	GCWGC	2 cut(s)	365, 1267
ApoI	RAATTY	2 cut(s)	434, 786
ArsI	GACNNNNNNTTYG	2 cut(s)	1320, 1352
AspS9I	GGNCC	4 cut(s)	425, 443, 569, 905
AsuHPI	GGTGA	4 cut(s)	244, 679, 854, 1081
AvaI	CYCGRG	1 cut(s)	820
AvaII	GGWCC	1 cut(s)	569
AxyI	CCTNAGG	2 cut(s)	630, 762
BanI	GGYRCC	1 cut(s)	400
BanII	GRGCYC	1 cut(s)	827
Bbv12I	GWGCWC	1 cut(s)	827
BbvCI	CCTCAGC	1 cut(s)	219
BbvI	GCAGC	2 cut(s)	377, 1254
BccI	CCATC	3 cut(s)	587, 776, 1106
BceAI	ACGGC	1 cut(s)	961
BciT130I	CCWGG	3 cut(s)	89, 573, 909
BclI	TGATCA	1 cut(s)	204
BcoDI	GTCTC	4 cut(s)	749, 876, 885, 1342
BcuI	ACTAGT	2 cut(s)	80, 559
BfaI	CTAG	4 cut(s)	81, 150, 521, 560
BfmI	CTRYAG	2 cut(s)	1234, 1283
BisI	GCNGC	2 cut(s)	366, 1268
BlsI	GCNGC	2 cut(s)	367, 1269
Bme1390I	CCNGG	3 cut(s)	89, 573, 909
Bme18I	GGWCC	1 cut(s)	569
BmeT110I	CYCGRG	1 cut(s)	820
BmgT120I	GGNCC	4 cut(s)	425, 443, 569, 905
BmiI	GGNNCC	5 cut(s)	402, 445, 481, 577, 1000
BmrFI	CCNGG	3 cut(s)	89, 573, 909
BmsI	GCATC	1 cut(s)	500
BplI	GAGNNNNNCTC	2 cut(s)	83, 115
Bpu10I	CCTNAGC	2 cut(s)	219, 1039
BsaI	GGTCTC	1 cut(s)	876
BsaJI	CCNNGG	1 cut(s)	913
BsaXI	ACNNNNNCTCC	4 cut(s)	375, 405, 960, 990
Bsc4I	CCNNNNNNNGG	3 cut(s)	648, 914, 1256
Bse1I	ACTGG	3 cut(s)	255, 378, 533
Bse21I	CCTNAGG	2 cut(s)	630, 762
Bse3DI	GCAATG	1 cut(s)	595
BseBI	CCWGG	3 cut(s)	89, 573, 909
BseDI	CCNNGG	1 cut(s)	913
BseLI	CCNNNNNNNGG	3 cut(s)	648, 914, 1256
BseMI	GCAATG	1 cut(s)	595
BseMII	CTCAG	6 cut(s)	233, 453, 644, 753, 1027, 1053
BseNI	ACTGG	3 cut(s)	255, 378, 533
BseXI	GCAGC	2 cut(s)	377, 1254
BsgI	GTGCAG	1 cut(s)	741
Bsh1236I	CGCG	1 cut(s)	1220
BshFI	GGCC	5 cut(s)	211, 427, 444, 907, 912
BshNI	GGYRCC	1 cut(s)	400
BsiHKAI	GWGCWC	1 cut(s)	827
BsiHKCI	CYCGRG	1 cut(s)	820
BslI	CCNNNNNNNGG	3 cut(s)	648, 914, 1256
BsmAI	GTCTC	4 cut(s)	749, 876, 885, 1342
BsmBI	CGTCTC	1 cut(s)	749
BsmI	GAATGC	1 cut(s)	1307
BsnI	GGCC	5 cut(s)	211, 427, 444, 907, 912
Bso31I	GGTCTC	1 cut(s)	876
BsoBI	CYCGRG	1 cut(s)	820
Bsp1286I	GDGCHC	1 cut(s)	827
Bsp143I	GATC	4 cut(s)	204, 503, 681, 1017
Bsp19I	CCATGG	1 cut(s)	913
BspACI	CCGC	2 cut(s)	1002, 1160
BspANI	GGCC	5 cut(s)	211, 427, 444, 907, 912
BspCNI	CTCAG	6 cut(s)	232, 452, 643, 754, 1026, 1052
BspFNI	CGCG	1 cut(s)	1220
BspLI	GGNNCC	5 cut(s)	402, 445, 481, 577, 1000
BspMAI	CTGCAG	1 cut(s)	1287
BspPI	GGATC	2 cut(s)	676, 1025
BspQI	GCTCTTC	2 cut(s)	230, 1288
BspT107I	GGYRCC	1 cut(s)	400
BspTNI	GGTCTC	1 cut(s)	876
BsrDI	GCAATG	1 cut(s)	595
BsrI	ACTGG	3 cut(s)	255, 378, 533
BssECI	CCNNGG	1 cut(s)	913
BssMI	GATC	4 cut(s)	204, 503, 681, 1017
BssT1I	CCWWGG	1 cut(s)	913
Bst2UI	CCWGG	3 cut(s)	89, 573, 909
Bst4CI	ACNGT	4 cut(s)	751, 851, 1121, 1235
Bst6I	CTCTTC	3 cut(s)	108, 230, 1288
BstC8I	GCNNGC	1 cut(s)	1320
BstDEI	CTNAG	6 cut(s)	219, 439, 630, 762, 1013, 1039
BstDSI	CCRYGG	1 cut(s)	913
BstENI	CCTNNNNNAGG	1 cut(s)	646
BstFNI	CGCG	1 cut(s)	1220
BstKTI	GATC	4 cut(s)	207, 506, 684, 1020
BstMAI	GTCTC	4 cut(s)	749, 876, 885, 1342
BstMBI	GATC	4 cut(s)	204, 503, 681, 1017
BstMWI	GCNNNNNNNGC	1 cut(s)	362
BstNI	CCWGG	3 cut(s)	89, 573, 909
BstNSI	RCATGY	1 cut(s)	707
BstSCI	CCNGG	3 cut(s)	87, 571, 907
BstSFI	CTRYAG	2 cut(s)	1234, 1283
BstUI	CGCG	1 cut(s)	1220
BstV1I	GCAGC	2 cut(s)	377, 1254
BstX2I	RGATCY	1 cut(s)	681
BstYI	RGATCY	1 cut(s)	681
Bsu36I	CCTNAGG	2 cut(s)	630, 762
BsuRI	GGCC	5 cut(s)	211, 427, 444, 907, 912
BtgI	CCRYGG	1 cut(s)	913
BtsI	GCAGTG	1 cut(s)	1051
BtsIMutI	CAGTG	4 cut(s)	262, 385, 526, 1051
Cac8I	GCNNGC	1 cut(s)	1320
CaiI	CAGNNNCTG	1 cut(s)	755
Cfr13I	GGNCC	4 cut(s)	425, 443, 569, 905
CseI	GACGC	2 cut(s)	698, 1226
Csp6I	GTAC	4 cut(s)	60, 78, 557, 1183
CviQI	GTAC	4 cut(s)	60, 78, 557, 1183
DdeI	CTNAG	6 cut(s)	219, 439, 630, 762, 1013, 1039
DpnI	GATC	4 cut(s)	206, 505, 683, 1019
DpnII	GATC	4 cut(s)	204, 503, 681, 1017
Eam1104I	CTCTTC	3 cut(s)	108, 230, 1288
EarI	CTCTTC	3 cut(s)	108, 230, 1288
Ecl136II	GAGCTC	1 cut(s)	825
Eco130I	CCWWGG	1 cut(s)	913
Eco24I	GRGCYC	1 cut(s)	827
Eco31I	GGTCTC	1 cut(s)	876
Eco47I	GGWCC	1 cut(s)	569
Eco53kI	GAGCTC	1 cut(s)	825
Eco57I	CTGAAG	2 cut(s)	211, 698
Eco81I	CCTNAGG	2 cut(s)	630, 762
Eco88I	CYCGRG	1 cut(s)	820
EcoICRI	GAGCTC	1 cut(s)	825
EcoNI	CCTNNNNNAGG	1 cut(s)	646
EcoO109I	RGGNCCY	1 cut(s)	443
EcoRI	GAATTC	2 cut(s)	434, 786
EcoRII	CCWGG	3 cut(s)	87, 571, 907
EcoT14I	CCWWGG	1 cut(s)	913
EcoT22I	ATGCAT	1 cut(s)	705
EcoT38I	GRGCYC	1 cut(s)	827
ErhI	CCWWGG	1 cut(s)	913
Esp3I	CGTCTC	1 cut(s)	749
FalI	AAGNNNNNCTT	2 cut(s)	1234, 1266
FauNDI	CATATG	1 cut(s)	599
FbaI	TGATCA	1 cut(s)	204
Fnu4HI	GCNGC	2 cut(s)	366, 1268
FriOI	GRGCYC	1 cut(s)	827
Fsp4HI	GCNGC	2 cut(s)	366, 1268
FspBI	CTAG	4 cut(s)	81, 150, 521, 560
GluI	GCNGC	2 cut(s)	366, 1268
HaeIII	GGCC	5 cut(s)	211, 427, 444, 907, 912
HgaI	GACGC	2 cut(s)	698, 1226
HincII	GTYRAC	3 cut(s)	97, 118, 301
HindII	GTYRAC	3 cut(s)	97, 118, 301
HindIII	AAGCTT	1 cut(s)	735
HinfI	GANTC	5 cut(s)	134, 673, 711, 918, 1011
HphI	GGTGA	4 cut(s)	244, 679, 854, 1081
Hpy166II	GTNNAC	3 cut(s)	97, 118, 301
Hpy188I	TCNGA	2 cut(s)	496, 678
Hpy188III	TCNNGA	7 cut(s)	456, 820, 966, 996, 1015, 1021, 1066
Hpy8I	GTNNAC	3 cut(s)	97, 118, 301
Hpy99I	CGWCG	1 cut(s)	1220
HpyAV	CCTTC	5 cut(s)	171, 176, 346, 355, 652
HpyCH4III	ACNGT	4 cut(s)	751, 851, 1121, 1235
HpyCH4IV	ACGT	4 cut(s)	114, 546, 555, 1226
HpyF10VI	GCNNNNNNNGC	1 cut(s)	362
HpyF3I	CTNAG	6 cut(s)	219, 439, 630, 762, 1013, 1039
HpySE526I	ACGT	4 cut(s)	114, 546, 555, 1226
Ksp22I	TGATCA	1 cut(s)	204
Kzo9I	GATC	4 cut(s)	204, 503, 681, 1017
LguI	GCTCTTC	2 cut(s)	230, 1288
LmnI	GCTCC	4 cut(s)	283, 311, 383, 983
Lsp1109I	GCAGC	2 cut(s)	377, 1254
LweI	GCATC	1 cut(s)	500
MaeI	CTAG	4 cut(s)	81, 150, 521, 560
MaeII	ACGT	4 cut(s)	114, 546, 555, 1226
MaeIII	GTNAC	6 cut(s)	110, 637, 751, 793, 1087, 1121
MalI	GATC	4 cut(s)	206, 505, 683, 1019
MboI	GATC	4 cut(s)	204, 503, 681, 1017
MboII	GAAGA	6 cut(s)	95, 217, 366, 682, 691, 1305
MfeI	CAATTG	1 cut(s)	429
MflI	RGATCY	1 cut(s)	681
MhlI	GDGCHC	1 cut(s)	827
MluCI	AATT	5 cut(s)	145, 200, 429, 434, 786
MlyI	GAGTC	2 cut(s)	143, 1005
MnlI	CCTC	6 cut(s)	85, 228, 639, 757, 1028, 1190
Mph1103I	ATGCAT	1 cut(s)	705
MseI	TTAA	2 cut(s)	1230, 1351
MspR9I	CCNGG	3 cut(s)	89, 573, 909
MunI	CAATTG	1 cut(s)	429
Mva1269I	GAATGC	1 cut(s)	1307
MvaI	CCWGG	3 cut(s)	89, 573, 909
MvnI	CGCG	1 cut(s)	1220
MwoI	GCNNNNNNNGC	1 cut(s)	362
NcoI	CCATGG	1 cut(s)	913
NdeI	CATATG	1 cut(s)	599
NdeII	GATC	4 cut(s)	204, 503, 681, 1017
NlaIV	GGNNCC	5 cut(s)	402, 445, 481, 577, 1000
NmuCI	GTSAC	3 cut(s)	637, 1087, 1121
NsiI	ATGCAT	1 cut(s)	705
NspI	RCATGY	1 cut(s)	707
PaeR7I	CTCGAG	1 cut(s)	820
PciSI	GCTCTTC	2 cut(s)	230, 1288
PcsI	WCGNNNNNNNCGW	1 cut(s)	552
PctI	GAATGC	1 cut(s)	1307
PfeI	GAWTC	3 cut(s)	673, 711, 918
PflFI	GACNNNGTC	1 cut(s)	545
PflMI	CCANNNNNTGG	1 cut(s)	914
PfoI	TCCNGGA	2 cut(s)	87, 571
PkrI	GCNGC	2 cut(s)	367, 1269
PleI	GAGTC	2 cut(s)	142, 1005
PpsI	GAGTC	2 cut(s)	142, 1005
Psp124BI	GAGCTC	1 cut(s)	827
Psp1406I	AACGTT	1 cut(s)	1226
Psp6I	CCWGG	3 cut(s)	87, 571, 907
PspGI	CCWGG	3 cut(s)	87, 571, 907
PspN4I	GGNNCC	5 cut(s)	402, 445, 481, 577, 1000
PspPI	GGNCC	4 cut(s)	425, 443, 569, 905
PstI	CTGCAG	1 cut(s)	1287
PstNI	CAGNNNCTG	1 cut(s)	755
PsuI	RGATCY	1 cut(s)	681
PsyI	GACNNNGTC	1 cut(s)	545
RsaI	GTAC	4 cut(s)	61, 79, 558, 1184
RsaNI	GTAC	4 cut(s)	60, 78, 557, 1183
SacI	GAGCTC	1 cut(s)	827
SapI	GCTCTTC	2 cut(s)	230, 1288
SaqAI	TTAA	2 cut(s)	1230, 1351
SatI	GCNGC	2 cut(s)	366, 1268
Sau3AI	GATC	4 cut(s)	204, 503, 681, 1017
Sau96I	GGNCC	4 cut(s)	425, 443, 569, 905
SchI	GAGTC	2 cut(s)	143, 1005
ScrFI	CCNGG	3 cut(s)	89, 573, 909
SduI	GDGCHC	1 cut(s)	827
SfaNI	GCATC	1 cut(s)	500
SfcI	CTRYAG	2 cut(s)	1234, 1283
Sfr274I	CTCGAG	1 cut(s)	820
SinI	GGWCC	1 cut(s)	569
SlaI	CTCGAG	1 cut(s)	820
SmlI	CTYRAG	1 cut(s)	820
SmoI	CTYRAG	1 cut(s)	820
SpeI	ACTAGT	2 cut(s)	80, 559
Sse9I	AATT	5 cut(s)	145, 200, 429, 434, 786
SsiI	CCGC	2 cut(s)	1002, 1160
SspI	AATATT	1 cut(s)	1276
SspMI	CTAG	4 cut(s)	81, 150, 521, 560
SstI	GAGCTC	1 cut(s)	827
StyD4I	CCNGG	3 cut(s)	87, 571, 907
StyI	CCWWGG	1 cut(s)	913
TaaI	ACNGT	4 cut(s)	751, 851, 1121, 1235
TaiI	ACGT	4 cut(s)	117, 549, 558, 1229
TaqI	TCGA	3 cut(s)	457, 821, 1107
TasI	AATT	5 cut(s)	145, 200, 429, 434, 786
TfiI	GAWTC	3 cut(s)	673, 711, 918
Tru1I	TTAA	2 cut(s)	1230, 1351
Tru9I	TTAA	2 cut(s)	1230, 1351
TscAI	CASTG	4 cut(s)	262, 385, 533, 1051
TseFI	GTSAC	3 cut(s)	637, 1087, 1121
TseI	GCWGC	2 cut(s)	365, 1267
Tsp45I	GTSAC	3 cut(s)	637, 1087, 1121
TspDTI	ATGAA	6 cut(s)	57, 95, 153, 184, 779, 1094
TspGWI	ACGGA	1 cut(s)	270
TspRI	CASTG	4 cut(s)	262, 385, 533, 1051
Tth111I	GACNNNGTC	1 cut(s)	545
Van91I	CCANNNNNTGG	1 cut(s)	914
VpaK11BI	GGWCC	1 cut(s)	569
XagI	CCTNNNNNAGG	1 cut(s)	646
XapI	RAATTY	2 cut(s)	434, 786
XceI	RCATGY	1 cut(s)	707
XhoI	CTCGAG	1 cut(s)	820
XspI	CTAG	4 cut(s)	81, 150, 521, 560
Zsp2I	ATGCAT	1 cut(s)	705

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.