Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003318

Physical Location & Seq

Genomic Coordinates Reverse (-)

9414496 .. 9418855

Gene Structure 4360 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1077400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1410 bp

ATGGCTCTTCAGAAAAATGAACTCATCCTATTCTTTATCATCACCCTCCCCTCCTTTGTTTTATGTTATAGCAGTTTACATGAGAACCAACTCATCGACAAGTATGTTGACGAAGCATCTGAATTTGATTCCAAAGCGTATCCATCTTACTTCAACACGATCGACGATGGCAGTAACTTGTTCAAGGGCTTGATAAAGGAAAGTGCAGATGTCCTATGCTTAAAGAGCTTCGATAAAGTGGATAGCAGCACTTCATCTTCGGCTGAGACGGTCAGTGTTAATGATTTTGGAGCTGAGGGTGATGGAGAAACTGATGACACAAAGGCTTTTGAGATGGCTTGGAAGGTAGCTTGTTCATCTAAGGAAGCTGTTATGGTGGCGCCTAAGAAGACGTATCGTATTAAACCAATCAGATTTTCAGGTCCTTGCAAATCTCAACTTACAGTGCAGATTCTGGGAACGTTAATAGCTTCTGATGATCGATCTGACTTCAGCAAAGACGGTAGACACTGGCTTATCTTTGACAGTGTTTCCAATTTGGTGGTTGAAGGTGGTGGGGCCATCAATGGCAATGGGAAAAATTGGTGGCAAAATTCATGCAAAACCGATAAATCTAAGCCCTGCAAGGACGCACCAACGGCACTCACCTTCTGTAAGAGCAAGAATTTGATGGTGAGGAACCTGACAATTCAAGATGGACAACAAATTCATGTGTCTTTCCAGAAATGCATGAATGTTGAAGTTTCCAATCTCTCAATAACTGCACCACCAAAAAGCCCCAACACCGACGGAATTCATGTTACAGATACTCAAAACATATTGATCACAAGCTGTGTTATAGCCACAGGTGATGATTGTCTTTCTATTGTAAATGGATCCAAAACGGTGCAAGCTTCGAACATAACCTGCGGATCAGGCCATGGTATCAGTATTGGAAGCCTGGGATCTGGAAACTCAAAAGCGTATGTTTCGGATATTACAGTGAATGGAGCAAAACTTTATGGAACTACAAATGGAATTCGAATCAAAACGTGGCAGGGAGGGTCAGGAAGTGCAAGCAACATCAGATTTCAGAACATTGAAATGCATAATGTTACCAACCCCATACTTATAGACCAATATTACTGTGACAGAAAGATACCGTGCAAAGAACAGAGATCAGCAGTTGAAGTCAAAAATGTGGTGTACAAAAACATCAAAGGAACAAGTGCTTCAGATGTTGCTATAAAATTCGATTGCAGCAAGAGCTTCCCTTGTCTGGGGATTATGTTGCAAGATATTAACCTCCAACACGAAGGAAGGAAAACAGCTAAAGCTCTGTGCAACAATGTTAATGTGACTAGCATAGGGGTTGTTTGCCCGCTTTGTCCTAAATTAGAAGGGCAATATGAGACTTGCCAATGTCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0004650 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0007275 GO:0008150 GO:0009664 GO:0009791 GO:0009827 GO:0009830 GO:0009838 GO:0009900 GO:0009901 GO:0009908 GO:0009987 GO:0010047 GO:0010227 GO:0016043 GO:0016787 GO:0016798 GO:0022411 GO:0022414 GO:0030312 GO:0032501 GO:0032502 GO:0032504 GO:0042545 GO:0044277 GO:0044464 GO:0045229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048608 GO:0048609 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0071944 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

Glyco_hydro_28

Protein Analysis

470

Amino Acids

50.97

Weight (kDa)

6.98

Isoelectric Point (pI)

28.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_28	PF00295	122 - 447	1.3e-101	Glycosyl hydrolases family 28

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003318)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41850
fragaria_vesca	FvH4_2g20930 FvH4_2g20930 FvH4_6g23580
malus_domestica	MD05G1192600.v1.1 MD10G1179100.v1.1 MD12G1077400.v1.1 MD12G1077600.v1.1
prunus_persica	Prupe.7G063900_v2.0.a1 Prupe.8G218900_v2.0.a1
pyrus_communis	pycom05g17960 pycom10g15530 pycom12g06230
rosa_chinensis	RchiOBHm_Chr3g0481691 RchiOBHm_Chr6g0287311
rosa_laevigata	RLG00000012502 RLG00000023383
rosa_multiflora	Rmu_sc0007029.1_g000032 Rmu_ssc0000267.1_g000001
rosa_roxburghii	Rroxscaffold_6G00399870 Rroxscaffold_6G00400230 Rroxscaffold_7G00181550
rosa_rugosa	Rorug03G0193400 Rorug06G0186800 Rorug06G0186900
rosa_samantha	Rh3AG244500 Rh3BG279600 Rh3CG274700 Rh3DG271500 Rh6AG298300 Rh6BG303700 Rh6CG306100 Rh6CG312000 Rh6DG295600
rosa_wichuraiana	Rw3G021820 Rw6G025780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	914
AccB1I	GGYRCC	1 cut(s)	379
AccI	GTMKAC	1 cut(s)	505
AciI	CCGC	2 cut(s)	909, 1361
AclI	AACGTT	1 cut(s)	461
AclWI	GGATC	4 cut(s)	870, 883, 919, 952
AcsI	RAATTY	7 cut(s)	122, 592, 664, 705, 792, 1017, 1229
AcuI	CTGAAG	2 cut(s)	475, 1197
AcyI	GRCGYC	1 cut(s)	380
AfaI	GTAC	1 cut(s)	1187
AfiI	CCNNNNNNNGG	2 cut(s)	1258, 1259
AgsI	TTSAA	7 cut(s)	154, 184, 548, 692, 740, 1082, 1169
AjnI	CCWGG	1 cut(s)	939
AjuI	GAANNNNNNNTTGG	2 cut(s)	400, 432
Alw26I	GTCTC	2 cut(s)	260, 1385
AlwI	GGATC	4 cut(s)	870, 883, 919, 952
AlwNI	CAGNNNCTG	1 cut(s)	454
AoxI	GGCC	2 cut(s)	558, 916
ApeKI	GCWGC	2 cut(s)	246, 1239
ApoI	RAATTY	7 cut(s)	122, 592, 664, 705, 792, 1017, 1229
AspLEI	GCGC	1 cut(s)	382
AspS9I	GGNCC	2 cut(s)	422, 558
AsuHPI	GGTGA	5 cut(s)	34, 311, 637, 685, 860
AsuII	TTCGAA	2 cut(s)	896, 1021
AvaII	GGWCC	1 cut(s)	422
BamHI	GGATCC	1 cut(s)	875
BanI	GGYRCC	1 cut(s)	379
BbsI	GAAGAC	1 cut(s)	395
BbvCI	CCTCAGC	1 cut(s)	294
BbvI	GCAGC	2 cut(s)	258, 1251
BccI	CCATC	7 cut(s)	151, 161, 296, 328, 569, 664, 689
BceAI	ACGGC	1 cut(s)	654
BciT130I	CCWGG	1 cut(s)	941
BciVI	GTATCC	1 cut(s)	150
BclI	TGATCA	1 cut(s)	822
BcoDI	GTCTC	2 cut(s)	260, 1385
BfaI	CTAG	1 cut(s)	1341
BfoI	RGCGCY	1 cut(s)	383
BfuAI	ACCTGC	1 cut(s)	914
BfuI	GTATCC	1 cut(s)	150
BisI	GCNGC	2 cut(s)	247, 1240
BlsI	GCNGC	2 cut(s)	248, 1241
Bme1390I	CCNGG	1 cut(s)	941
Bme18I	GGWCC	1 cut(s)	422
BmgT120I	GGNCC	2 cut(s)	422, 558
BmiI	GGNNCC	4 cut(s)	381, 559, 680, 877
BmrFI	CCNGG	1 cut(s)	941
BmsI	GCATC	1 cut(s)	125
BpiI	GAAGAC	1 cut(s)	395
Bpu10I	CCTNAGC	1 cut(s)	294
Bpu14I	TTCGAA	2 cut(s)	896, 1021
Bsa29I	ATCGAT	1 cut(s)	481
BsaHI	GRCGYC	1 cut(s)	380
BsaJI	CCNNGG	2 cut(s)	919, 940
Bsc4I	CCNNNNNNNGG	2 cut(s)	1258, 1259
Bse1I	ACTGG	1 cut(s)	515
Bse3DI	GCAATG	1 cut(s)	577
BseBI	CCWGG	1 cut(s)	941
BseCI	ATCGAT	1 cut(s)	481
BseDI	CCNNGG	2 cut(s)	919, 940
BseGI	GGATG	1 cut(s)	24
BseLI	CCNNNNNNNGG	2 cut(s)	1258, 1259
BseMI	GCAATG	1 cut(s)	577
BseMII	CTCAG	2 cut(s)	255, 285
BseNI	ACTGG	1 cut(s)	515
BseXI	GCAGC	2 cut(s)	258, 1251
BsgI	GTGCAG	3 cut(s)	225, 467, 747
Bsh1285I	CGRYCG	1 cut(s)	162
BshFI	GGCC	2 cut(s)	560, 918
BshNI	GGYRCC	1 cut(s)	379
BshVI	ATCGAT	1 cut(s)	481
BsiEI	CGRYCG	1 cut(s)	162
BslI	CCNNNNNNNGG	2 cut(s)	1258, 1259
BsmAI	GTCTC	2 cut(s)	260, 1385
BsmBI	CGTCTC	1 cut(s)	260
BsnI	GGCC	2 cut(s)	560, 918
Bsp119I	TTCGAA	2 cut(s)	896, 1021
Bsp1407I	TGTACA	1 cut(s)	1185
Bsp143I	GATC	8 cut(s)	159, 478, 482, 822, 875, 911, 944, 1157
Bsp19I	CCATGG	1 cut(s)	919
BspACI	CCGC	2 cut(s)	909, 1361
BspANI	GGCC	2 cut(s)	560, 918
BspCNI	CTCAG	2 cut(s)	256, 286
BspDI	ATCGAT	1 cut(s)	481
BspLI	GGNNCC	4 cut(s)	381, 559, 680, 877
BspMI	ACCTGC	1 cut(s)	914
BspPI	GGATC	4 cut(s)	870, 883, 919, 952
BspQI	GCTCTTC	1 cut(s)	12
BspT104I	TTCGAA	2 cut(s)	896, 1021
BspT107I	GGYRCC	1 cut(s)	379
BsrDI	GCAATG	1 cut(s)	577
BsrGI	TGTACA	1 cut(s)	1185
BsrI	ACTGG	1 cut(s)	515
BssECI	CCNNGG	2 cut(s)	919, 940
BssMI	GATC	8 cut(s)	159, 478, 482, 822, 875, 911, 944, 1157
BssNI	GRCGYC	1 cut(s)	380
BssT1I	CCWWGG	1 cut(s)	919
Bst2UI	CCWGG	1 cut(s)	941
Bst4CI	ACNGT	8 cut(s)	271, 445, 503, 527, 886, 982, 1127, 1143
Bst6I	CTCTTC	1 cut(s)	12
BstACI	GRCGYC	1 cut(s)	380
BstAUI	TGTACA	1 cut(s)	1185
BstBI	TTCGAA	2 cut(s)	896, 1021
BstC8I	GCNNGC	3 cut(s)	891, 1057, 1361
BstDEI	CTNAG	5 cut(s)	264, 294, 360, 384, 615
BstDSI	CCRYGG	1 cut(s)	919
BstF5I	GGATG	1 cut(s)	24
BstH2I	RGCGCY	1 cut(s)	383
BstHHI	GCGC	1 cut(s)	382
BstKTI	GATC	8 cut(s)	162, 481, 485, 825, 878, 914, 947, 1160
BstMAI	GTCTC	2 cut(s)	260, 1385
BstMBI	GATC	8 cut(s)	159, 478, 482, 822, 875, 911, 944, 1157
BstMCI	CGRYCG	1 cut(s)	162
BstMWI	GCNNNNNNNGC	4 cut(s)	225, 638, 915, 1245
BstNI	CCWGG	1 cut(s)	941
BstSCI	CCNGG	1 cut(s)	939
BstV1I	GCAGC	2 cut(s)	258, 1251
BstV2I	GAAGAC	1 cut(s)	395
BstX2I	RGATCY	2 cut(s)	875, 944
BstXI	CCANNNNNNTGG	1 cut(s)	541
BstYI	RGATCY	2 cut(s)	875, 944
Bsu15I	ATCGAT	1 cut(s)	481
BsuI	GTATCC	1 cut(s)	150
BsuRI	GGCC	2 cut(s)	560, 918
BsuTUI	ATCGAT	1 cut(s)	481
BtgI	CCRYGG	1 cut(s)	919
BtsCI	GGATG	1 cut(s)	24
BtsIMutI	CAGTG	5 cut(s)	280, 450, 508, 532, 987
BveI	ACCTGC	1 cut(s)	914
Cac8I	GCNNGC	3 cut(s)	891, 1057, 1361
CaiI	CAGNNNCTG	1 cut(s)	454
CfoI	GCGC	1 cut(s)	382
Cfr13I	GGNCC	2 cut(s)	422, 558
ClaI	ATCGAT	1 cut(s)	481
CseI	GACGC	1 cut(s)	638
Csp6I	GTAC	1 cut(s)	1186
CviAII	CATG	6 cut(s)	80, 597, 710, 730, 797, 920
CviQI	GTAC	1 cut(s)	1186
DdeI	CTNAG	5 cut(s)	264, 294, 360, 384, 615
DinI	GGCGCC	1 cut(s)	381
DpnI	GATC	8 cut(s)	161, 480, 484, 824, 877, 913, 946, 1159
DpnII	GATC	8 cut(s)	159, 478, 482, 822, 875, 911, 944, 1157
Eam1104I	CTCTTC	1 cut(s)	12
EarI	CTCTTC	1 cut(s)	12
Eco130I	CCWWGG	1 cut(s)	919
Eco47I	GGWCC	1 cut(s)	422
Eco57I	CTGAAG	2 cut(s)	475, 1197
EcoO109I	RGGNCCY	1 cut(s)	422
EcoRI	GAATTC	2 cut(s)	792, 1017
EcoRII	CCWGG	1 cut(s)	939
EcoT14I	CCWWGG	1 cut(s)	919
EcoT22I	ATGCAT	2 cut(s)	731, 1089
EgeI	GGCGCC	1 cut(s)	381
EheI	GGCGCC	1 cut(s)	381
ErhI	CCWWGG	1 cut(s)	919
Esp3I	CGTCTC	1 cut(s)	260
FaeI	CATG	6 cut(s)	83, 600, 713, 733, 800, 923
FatI	CATG	6 cut(s)	79, 596, 709, 729, 796, 919
FauI	CCCGC	1 cut(s)	1368
FbaI	TGATCA	1 cut(s)	822
FblI	GTMKAC	1 cut(s)	505
Fnu4HI	GCNGC	2 cut(s)	247, 1240
FokI	GGATG	1 cut(s)	11
Fsp4HI	GCNGC	2 cut(s)	247, 1240
FspBI	CTAG	1 cut(s)	1341
GlaI	GCGC	1 cut(s)	381
GluI	GCNGC	2 cut(s)	247, 1240
HaeII	RGCGCY	1 cut(s)	383
HaeIII	GGCC	2 cut(s)	560, 918
HgaI	GACGC	1 cut(s)	638
HhaI	GCGC	1 cut(s)	382
Hin1I	GRCGYC	1 cut(s)	380
Hin1II	CATG	6 cut(s)	83, 600, 713, 733, 800, 923
Hin6I	GCGC	1 cut(s)	380
HinP1I	GCGC	1 cut(s)	380
HincII	GTYRAC	1 cut(s)	109
HindII	GTYRAC	1 cut(s)	109
HindIII	AAGCTT	1 cut(s)	891
HinfI	GANTC	3 cut(s)	128, 451, 1023
HphI	GGTGA	5 cut(s)	34, 311, 637, 685, 860
Hpy166II	GTNNAC	4 cut(s)	77, 109, 506, 1186
Hpy188I	TCNGA	9 cut(s)	12, 121, 413, 475, 487, 973, 1067, 1074, 1216
Hpy188III	TCNNGA	4 cut(s)	692, 721, 948, 1047
Hpy8I	GTNNAC	4 cut(s)	77, 109, 506, 1186
Hpy99I	CGWCG	2 cut(s)	167, 791
HpyAV	CCTTC	6 cut(s)	337, 542, 658, 1289, 1293, 1373
HpyCH4III	ACNGT	8 cut(s)	271, 445, 503, 527, 886, 982, 1127, 1143
HpyCH4IV	ACGT	3 cut(s)	392, 461, 1031
HpyF10VI	GCNNNNNNNGC	4 cut(s)	225, 638, 915, 1245
HpyF3I	CTNAG	5 cut(s)	264, 294, 360, 384, 615
HpySE526I	ACGT	3 cut(s)	392, 461, 1031
Hsp92I	GRCGYC	1 cut(s)	380
Hsp92II	CATG	6 cut(s)	83, 600, 713, 733, 800, 923
HspAI	GCGC	1 cut(s)	380
KasI	GGCGCC	1 cut(s)	379
Ksp22I	TGATCA	1 cut(s)	822
Kzo9I	GATC	8 cut(s)	159, 478, 482, 822, 875, 911, 944, 1157
LguI	GCTCTTC	1 cut(s)	12
LmnI	GCTCC	2 cut(s)	290, 989
Lsp1109I	GCAGC	2 cut(s)	258, 1251
LweI	GCATC	1 cut(s)	125
MaeI	CTAG	1 cut(s)	1341
MaeII	ACGT	3 cut(s)	392, 461, 1031
MaeIII	GTNAC	5 cut(s)	173, 799, 1093, 1127, 1336
MalI	GATC	8 cut(s)	161, 480, 484, 824, 877, 913, 946, 1159
MboI	GATC	8 cut(s)	159, 478, 482, 822, 875, 911, 944, 1157
MboII	GAAGA	2 cut(s)	249, 400
MflI	RGATCY	2 cut(s)	875, 944
Mly113I	GGCGCC	1 cut(s)	380
MmeI	TCCRAC	1 cut(s)	1312
MnlI	CCTC	6 cut(s)	56, 61, 289, 669, 1034, 1295
Mph1103I	ATGCAT	2 cut(s)	731, 1089
MseI	TTAA	6 cut(s)	221, 279, 402, 464, 1281, 1332
MslI	CAYNNNNRTG	1 cut(s)	1082
MspR9I	CCNGG	1 cut(s)	941
MvaI	CCWGG	1 cut(s)	941
MwoI	GCNNNNNNNGC	4 cut(s)	225, 638, 915, 1245
NarI	GGCGCC	1 cut(s)	380
NcoI	CCATGG	1 cut(s)	919
NdeII	GATC	8 cut(s)	159, 478, 482, 822, 875, 911, 944, 1157
NlaIII	CATG	6 cut(s)	83, 600, 713, 733, 800, 923
NlaIV	GGNNCC	4 cut(s)	381, 559, 680, 877
NmuCI	GTSAC	2 cut(s)	1127, 1336
NsiI	ATGCAT	2 cut(s)	731, 1089
NspV	TTCGAA	2 cut(s)	896, 1021
PciSI	GCTCTTC	1 cut(s)	12
PfeI	GAWTC	3 cut(s)	128, 451, 1023
PkrI	GCNGC	2 cut(s)	248, 1241
Ple19I	CGATCG	1 cut(s)	162
PluTI	GGCGCC	1 cut(s)	383
PpuMI	RGGWCCY	1 cut(s)	422
Psp1406I	AACGTT	1 cut(s)	461
Psp5II	RGGWCCY	1 cut(s)	422
Psp6I	CCWGG	1 cut(s)	939
PspGI	CCWGG	1 cut(s)	939
PspN4I	GGNNCC	4 cut(s)	381, 559, 680, 877
PspPI	GGNCC	2 cut(s)	422, 558
PspPPI	RGGWCCY	1 cut(s)	422
PstNI	CAGNNNCTG	1 cut(s)	454
PsuI	RGATCY	2 cut(s)	875, 944
PvuI	CGATCG	1 cut(s)	162
RsaI	GTAC	1 cut(s)	1187
RsaNI	GTAC	1 cut(s)	1186
RseI	CAYNNNNRTG	1 cut(s)	1082
SapI	GCTCTTC	1 cut(s)	12
SaqAI	TTAA	6 cut(s)	221, 279, 402, 464, 1281, 1332
SatI	GCNGC	2 cut(s)	247, 1240
Sau3AI	GATC	8 cut(s)	159, 478, 482, 822, 875, 911, 944, 1157
Sau96I	GGNCC	2 cut(s)	422, 558
ScrFI	CCNGG	1 cut(s)	941
SfaNI	GCATC	1 cut(s)	125
SfoI	GGCGCC	1 cut(s)	381
SfuI	TTCGAA	2 cut(s)	896, 1021
SinI	GGWCC	1 cut(s)	422
SmiMI	CAYNNNNRTG	1 cut(s)	1082
SsiI	CCGC	2 cut(s)	909, 1361
SspDI	GGCGCC	1 cut(s)	379
SspI	AATATT	1 cut(s)	1121
SspMI	CTAG	1 cut(s)	1341
StyD4I	CCNGG	1 cut(s)	939
StyI	CCWWGG	1 cut(s)	919
TaaI	ACNGT	8 cut(s)	271, 445, 503, 527, 886, 982, 1127, 1143
TaiI	ACGT	3 cut(s)	395, 464, 1034
TaqI	TCGA	7 cut(s)	96, 162, 231, 481, 896, 1021, 1233
TatI	WGTACW	1 cut(s)	1185
TfiI	GAWTC	3 cut(s)	128, 451, 1023
Tru1I	TTAA	6 cut(s)	221, 279, 402, 464, 1281, 1332
Tru9I	TTAA	6 cut(s)	221, 279, 402, 464, 1281, 1332
TscAI	CASTG	5 cut(s)	280, 450, 515, 532, 987
TseFI	GTSAC	2 cut(s)	1127, 1336
TseI	GCWGC	2 cut(s)	246, 1239
Tsp45I	GTSAC	2 cut(s)	1127, 1336
TspDTI	ATGAA	7 cut(s)	33, 243, 345, 585, 698, 746, 785
TspGWI	ACGGA	1 cut(s)	804
TspRI	CASTG	5 cut(s)	280, 450, 515, 532, 987
VpaK11BI	GGWCC	1 cut(s)	422
XapI	RAATTY	7 cut(s)	122, 592, 664, 705, 792, 1017, 1229
XmiI	GTMKAC	1 cut(s)	505
XspI	CTAG	1 cut(s)	1341
Zsp2I	ATGCAT	2 cut(s)	731, 1089

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.