MD10G1179100.v1.1

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003318
Physical Location & Seq
Forward (+)
27285865 .. 27288648
2784 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1179100.v1.1.491

Sequence Viewer

Length: 1383 bp
ATGGCTTTAAAAACACAGTTGTTGTGGTCATTTGTTGTTGTTTTTGTTGTTTCCTTCAGTACAACTTCATGTTCTGGTAGTAGTTTCCAGGAGGTCAACGCGCTTCATAGTTACGTTGACCATGTTGATGATAAAGAGTCCGGCTATAATTCTAGGGCTTATCCTTCATACACGGACACCATAGAAGGTTTAAAGTTCATGGAATTGATCAGGCCAAGAACTCAGCTCTTCAGTTCAAGGAAGCTCAACACAATCACCGGTGGGATAGCAACATCATCAGCTCCGGCCAAAACCATTAGCGTCGACGATTTTGGAGCTAAAGGGAATGGTGCTGATGACACACAGGCATTTGTGAAGGCATGGAAGGCAGCTTGTTCTTCCAGTGGAGCTATGGTTCTTGTGGTACCACGGAAGAACTATCTTGTTAGGCCGATTGAATTCTCAGGCCCATGCAAATCTCAACTTACACTGCAGATTTATGGAACCATAGAAGCATCAGAAGACCGATCAATCTACAAAGACATAGACCACTGGCTCATCTTTGACAATGTCCAAAACTTGCTAGTTGTTGGTCCTGGAACCATCAATGGCAATGGAAACATCTGGTGGAAAAACTCATGCAAAATAAAACCTCAGCCCCCTTGCGGTACATACGCCCCCACAGCTGTGACCTTCAACAGGTGCAATAACTTGGTGGTGAAGAATCTGAATATCCAAGACGCACAACAAATCCATGTCATATTCCAAAACTGCATCAACGTTCAAGCTTCCCGTCTCACGGTAACTGCACCAGAGGACAGCCCTAATACGGACGGAATTCATGTGACAAATACCCAGAACATCACTATCTCGAGCTCGGTTATAGGAACAGGTGATGACTGTATTTCTATTGTGAGTGGGTCCCAAAGAGTTCAAGCCACAGACATTACTTGTGGACCAGGCCATGGAATCAGTATCGGTAGCTTGGGAGAAGACGGCTCAGAAGATCATGTTTCAGGAGTATTTGTGAATGGAGCTAAGCTTTCAGGAACCTCCAATGGACTCCGGATCAAGACGTGGAAGGGAGGCTCAGGCAGTGTAACCAACATTGTTTTCCAGAATGTGCAAATGAACGATGTCACCAACCCCATCATCATCGACCAGAACTACTGTGACCACAAAACCAAAGATTGCAAACAACAGAAATCGGCGGTTCAAGTGAAAAATGTGTTGTACCAAAACATAAGAGGAACGAGTGCTTCCGGCGACGCGATAACGTTGAACTGCAGCCAAAGTGTTCCTTGTCAGGGGATCGTGCTGCAAAGTGTTCAACTGCAGAATGGAAGAGCTGAATGCAACAATGTTCAGCCTGCTTACAAAGGAGTTGTCTCCCCTAGATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

461

Amino Acids

49.56

Weight (kDa)

7.85

Isoelectric Point (pI)

33.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 125 - 449 1.1e-100 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 403
AccB1I GGYRCC 1 cut(s) 403
AccB7I CCANNNNNTGG 1 cut(s) 944
AccI GTMKAC 1 cut(s) 303
AccII CGCG 2 cut(s) 101, 1250
AccIII TCCGGA 1 cut(s) 1044
AciI CCGC 2 cut(s) 645, 1190
AclI AACGTT 2 cut(s) 759, 1256
AclWI GGATC 2 cut(s) 1055, 1298
AcoI YGGCCR 1 cut(s) 285
AcsI RAATTY 2 cut(s) 437, 816
AcuI CTGAAG 2 cut(s) 40, 214
AfaI GTAC 4 cut(s) 61, 405, 649, 1214
AfiI CCNNNNNNNGG 6 cut(s) 644, 678, 778, 808, 944, 1286
AgeI ACCGGT 1 cut(s) 257
AgsI TTSAA 8 cut(s) 237, 437, 676, 764, 914, 1196, 1261, 1310
AjiI CACGTC 1 cut(s) 1056
AjnI CCWGG 3 cut(s) 87, 574, 937
AjuI GAANNNNNNNTTGG 2 cut(s) 1076, 1108
AleI CACNNNNGTG 1 cut(s) 665
AloI GAACNNNNNNTCC 2 cut(s) 378, 410
Alw21I GWGCWC 1 cut(s) 857
Alw26I GTCTC 2 cut(s) 779, 1372
AlwI GGATC 2 cut(s) 1055, 1298
Ama87I CYCGRG 1 cut(s) 850
Aor13HI TCCGGA 1 cut(s) 1044
AoxI GGCC 5 cut(s) 212, 285, 428, 445, 940
ApeKI GCWGC 3 cut(s) 368, 1266, 1297
ApoI RAATTY 2 cut(s) 437, 816
ArsI GACNNNNNNTTYG 2 cut(s) 1350, 1382
AsiGI ACCGGT 1 cut(s) 257
Asp718I GGTACC 1 cut(s) 403
AspLEI GCGC 1 cut(s) 103
AspS9I GGNCC 4 cut(s) 446, 572, 900, 935
AsuHPI GGTGA 4 cut(s) 247, 709, 884, 1111
AvaI CYCGRG 1 cut(s) 850
AvaII GGWCC 3 cut(s) 572, 900, 935
BanI GGYRCC 1 cut(s) 403
BanII GRGCYC 1 cut(s) 857
BbsI GAAGAC 2 cut(s) 507, 978
Bbv12I GWGCWC 1 cut(s) 857
BbvCI CCTCAGC 1 cut(s) 633
BbvI GCAGC 3 cut(s) 380, 1278, 1284
BccI CCATC 2 cut(s) 590, 1136
BceAI ACGGC 1 cut(s) 991
BciT130I CCWGG 3 cut(s) 89, 576, 939
BclI TGATCA 1 cut(s) 207
BcoDI GTCTC 2 cut(s) 779, 1372
BfaI CTAG 3 cut(s) 153, 563, 1374
BfmI CTRYAG 3 cut(s) 470, 1264, 1313
BisI GCNGC 3 cut(s) 369, 1267, 1298
BlpI GCTNAGC 1 cut(s) 1017
BlsI GCNGC 3 cut(s) 370, 1268, 1299
Bme1390I CCNGG 3 cut(s) 89, 576, 939
Bme18I GGWCC 3 cut(s) 572, 900, 935
BmeT110I CYCGRG 1 cut(s) 850
BmgBI CACGTC 1 cut(s) 1056
BmgT120I GGNCC 4 cut(s) 446, 572, 900, 935
BmiI GGNNCC 6 cut(s) 405, 484, 580, 901, 902, 1030
BmrFI CCNGG 3 cut(s) 89, 576, 939
BmsI GCATC 2 cut(s) 503, 762
BpiI GAAGAC 2 cut(s) 507, 978
Bpu10I CCTNAGC 2 cut(s) 633, 1069
Bpu1102I GCTNAGC 1 cut(s) 1017
BsaJI CCNNGG 2 cut(s) 407, 943
BsaWI WCCGGW 2 cut(s) 257, 1044
BsaXI ACNNNNNCTCC 4 cut(s) 378, 408, 990, 1020
Bsc4I CCNNNNNNNGG 6 cut(s) 644, 678, 778, 808, 944, 1286
Bse118I RCCGGY 1 cut(s) 257
Bse1I ACTGG 2 cut(s) 381, 536
Bse3DI GCAATG 1 cut(s) 598
BseAI TCCGGA 1 cut(s) 1044
BseBI CCWGG 3 cut(s) 89, 576, 939
BseDI CCNNGG 2 cut(s) 407, 943
BseLI CCNNNNNNNGG 6 cut(s) 644, 678, 778, 808, 944, 1286
BseMI GCAATG 1 cut(s) 598
BseMII CTCAG 5 cut(s) 236, 456, 647, 993, 1083
BseNI ACTGG 2 cut(s) 381, 536
BseXI GCAGC 3 cut(s) 380, 1278, 1284
BsgI GTGCAG 1 cut(s) 771
Bsh1236I CGCG 2 cut(s) 101, 1250
BshFI GGCC 5 cut(s) 214, 287, 430, 447, 942
BshNI GGYRCC 1 cut(s) 403
BshTI ACCGGT 1 cut(s) 257
BsiHKAI GWGCWC 1 cut(s) 857
BsiHKCI CYCGRG 1 cut(s) 850
BsiSI CCGG 5 cut(s) 141, 258, 284, 1045, 1242
BslFI GGGAC 1 cut(s) 886
BslI CCNNNNNNNGG 6 cut(s) 644, 678, 778, 808, 944, 1286
BsmAI GTCTC 2 cut(s) 779, 1372
BsmBI CGTCTC 1 cut(s) 779
BsmFI GGGAC 1 cut(s) 886
BsmI GAATGC 1 cut(s) 1337
BsnI GGCC 5 cut(s) 214, 287, 430, 447, 942
BsoBI CYCGRG 1 cut(s) 850
Bsp1286I GDGCHC 1 cut(s) 857
Bsp13I TCCGGA 1 cut(s) 1044
Bsp143I GATC 5 cut(s) 207, 506, 985, 1047, 1290
Bsp1720I GCTNAGC 1 cut(s) 1017
Bsp19I CCATGG 1 cut(s) 943
BspACI CCGC 2 cut(s) 645, 1190
BspANI GGCC 5 cut(s) 214, 287, 430, 447, 942
BspCNI CTCAG 5 cut(s) 235, 455, 646, 992, 1082
BspEI TCCGGA 1 cut(s) 1044
BspFNI CGCG 2 cut(s) 101, 1250
BspLI GGNNCC 6 cut(s) 405, 484, 580, 901, 902, 1030
BspMAI CTGCAG 3 cut(s) 474, 1268, 1317
BspPI GGATC 2 cut(s) 1055, 1298
BspQI GCTCTTC 2 cut(s) 233, 1318
BspT107I GGYRCC 1 cut(s) 403
BsrDI GCAATG 1 cut(s) 598
BsrFI RCCGGY 1 cut(s) 257
BsrI ACTGG 2 cut(s) 381, 536
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 2 cut(s) 407, 943
BssMI GATC 5 cut(s) 207, 506, 985, 1047, 1290
BssT1I CCWWGG 1 cut(s) 943
Bst2UI CCWGG 3 cut(s) 89, 576, 939
Bst4CI ACNGT 4 cut(s) 18, 781, 881, 1151
Bst6I CTCTTC 2 cut(s) 233, 1318
BstC8I GCNNGC 1 cut(s) 1350
BstDEI CTNAG 6 cut(s) 222, 442, 633, 979, 1017, 1069
BstDSI CCRYGG 2 cut(s) 407, 943
BstENI CCTNNNNNAGG 1 cut(s) 676
BstFNI CGCG 2 cut(s) 101, 1250
BstHHI GCGC 1 cut(s) 103
BstKTI GATC 5 cut(s) 210, 509, 988, 1050, 1293
BstMAI GTCTC 2 cut(s) 779, 1372
BstMBI GATC 5 cut(s) 207, 506, 985, 1047, 1290
BstMWI GCNNNNNNNGC 2 cut(s) 365, 662
BstNI CCWGG 3 cut(s) 89, 576, 939
BstSCI CCNGG 3 cut(s) 87, 574, 937
BstSFI CTRYAG 3 cut(s) 470, 1264, 1313
BstUI CGCG 2 cut(s) 101, 1250
BstV1I GCAGC 3 cut(s) 380, 1278, 1284
BstV2I GAAGAC 2 cut(s) 507, 978
BsuRI GGCC 5 cut(s) 214, 287, 430, 447, 942
BtgI CCRYGG 2 cut(s) 407, 943
BtrI CACGTC 1 cut(s) 1056
BtsI GCAGTG 2 cut(s) 467, 1081
BtsIMutI CAGTG 4 cut(s) 388, 467, 529, 1081
Cac8I GCNNGC 1 cut(s) 1350
CfoI GCGC 1 cut(s) 103
Cfr10I RCCGGY 1 cut(s) 257
Cfr13I GGNCC 4 cut(s) 446, 572, 900, 935
CseI GACGC 3 cut(s) 289, 728, 1256
Csp6I GTAC 4 cut(s) 60, 404, 648, 1213
CspAI ACCGGT 1 cut(s) 257
CviQI GTAC 4 cut(s) 60, 404, 648, 1213
DdeI CTNAG 6 cut(s) 222, 442, 633, 979, 1017, 1069
DpnI GATC 5 cut(s) 209, 508, 987, 1049, 1292
DpnII GATC 5 cut(s) 207, 506, 985, 1047, 1290
DraI TTTAAA 2 cut(s) 9, 192
EaeI YGGCCR 1 cut(s) 285
Eam1104I CTCTTC 2 cut(s) 233, 1318
EarI CTCTTC 2 cut(s) 233, 1318
Ecl136II GAGCTC 1 cut(s) 855
Eco130I CCWWGG 1 cut(s) 943
Eco24I GRGCYC 1 cut(s) 857
Eco47I GGWCC 3 cut(s) 572, 900, 935
Eco53kI GAGCTC 1 cut(s) 855
Eco57I CTGAAG 2 cut(s) 40, 214
Eco88I CYCGRG 1 cut(s) 850
EcoICRI GAGCTC 1 cut(s) 855
EcoNI CCTNNNNNAGG 1 cut(s) 676
EcoO109I RGGNCCY 1 cut(s) 900
EcoRI GAATTC 2 cut(s) 437, 816
EcoRII CCWGG 3 cut(s) 87, 574, 937
EcoT14I CCWWGG 1 cut(s) 943
EcoT38I GRGCYC 1 cut(s) 857
ErhI CCWWGG 1 cut(s) 943
Esp3I CGTCTC 1 cut(s) 779
FalI AAGNNNNNCTT 2 cut(s) 1264, 1296
FaqI GGGAC 1 cut(s) 886
FbaI TGATCA 1 cut(s) 207
FblI GTMKAC 1 cut(s) 303
Fnu4HI GCNGC 3 cut(s) 369, 1267, 1298
FriOI GRGCYC 1 cut(s) 857
Fsp4HI GCNGC 3 cut(s) 369, 1267, 1298
FspBI CTAG 3 cut(s) 153, 563, 1374
GlaI GCGC 1 cut(s) 102
GluI GCNGC 3 cut(s) 369, 1267, 1298
HaeIII GGCC 5 cut(s) 214, 287, 430, 447, 942
HapII CCGG 5 cut(s) 141, 258, 284, 1045, 1242
HgaI GACGC 3 cut(s) 289, 728, 1256
HhaI GCGC 1 cut(s) 103
Hin6I GCGC 1 cut(s) 101
HinP1I GCGC 1 cut(s) 101
HincII GTYRAC 3 cut(s) 97, 118, 304
HindII GTYRAC 3 cut(s) 97, 118, 304
HindIII AAGCTT 2 cut(s) 765, 1019
HinfI GANTC 4 cut(s) 137, 703, 948, 1041
HpaII CCGG 5 cut(s) 141, 258, 284, 1045, 1242
HphI GGTGA 4 cut(s) 247, 709, 884, 1111
Hpy166II GTNNAC 4 cut(s) 97, 118, 304, 935
Hpy188I TCNGA 3 cut(s) 499, 708, 982
Hpy188III TCNNGA 6 cut(s) 850, 996, 1026, 1045, 1051, 1096
Hpy8I GTNNAC 4 cut(s) 97, 118, 304, 935
Hpy99I CGWCG 3 cut(s) 305, 308, 1250
HpyAV CCTTC 7 cut(s) 64, 174, 179, 349, 358, 682, 1054
HpyCH4III ACNGT 4 cut(s) 18, 781, 881, 1151
HpyCH4IV ACGT 4 cut(s) 114, 759, 1055, 1256
HpyF10VI GCNNNNNNNGC 2 cut(s) 365, 662
HpyF3I CTNAG 6 cut(s) 222, 442, 633, 979, 1017, 1069
HpySE526I ACGT 4 cut(s) 114, 759, 1055, 1256
HspAI GCGC 1 cut(s) 101
KflI GGGWCCC 1 cut(s) 900
Kpn2I TCCGGA 1 cut(s) 1044
KpnI GGTACC 1 cut(s) 407
Ksp22I TGATCA 1 cut(s) 207
Kzo9I GATC 5 cut(s) 207, 506, 985, 1047, 1290
LguI GCTCTTC 2 cut(s) 233, 1318
LmnI GCTCC 4 cut(s) 286, 314, 386, 1013
Lsp1109I GCAGC 3 cut(s) 380, 1278, 1284
LweI GCATC 2 cut(s) 503, 762
MaeI CTAG 3 cut(s) 153, 563, 1374
MaeII ACGT 4 cut(s) 114, 759, 1055, 1256
MaeIII GTNAC 7 cut(s) 110, 667, 781, 823, 1078, 1117, 1151
MalI GATC 5 cut(s) 209, 508, 987, 1049, 1292
MboI GATC 5 cut(s) 207, 506, 985, 1047, 1290
MboII GAAGA 8 cut(s) 220, 369, 424, 512, 712, 983, 995, 1335
MhlI GDGCHC 1 cut(s) 857
MluCI AATT 4 cut(s) 148, 203, 437, 816
MlyI GAGTC 2 cut(s) 146, 1035
MnlI CCTC 6 cut(s) 85, 642, 787, 1042, 1058, 1220
MroI TCCGGA 1 cut(s) 1044
MseI TTAA 3 cut(s) 8, 191, 1381
MslI CAYNNNNRTG 2 cut(s) 126, 665
MspA1I CMGCKG 1 cut(s) 665
MspI CCGG 5 cut(s) 141, 258, 284, 1045, 1242
MspR9I CCNGG 3 cut(s) 89, 576, 939
Mva1269I GAATGC 1 cut(s) 1337
MvaI CCWGG 3 cut(s) 89, 576, 939
MvnI CGCG 2 cut(s) 101, 1250
MwoI GCNNNNNNNGC 2 cut(s) 365, 662
NcoI CCATGG 1 cut(s) 943
NdeII GATC 5 cut(s) 207, 506, 985, 1047, 1290
NlaIV GGNNCC 6 cut(s) 405, 484, 580, 901, 902, 1030
NmuCI GTSAC 4 cut(s) 667, 823, 1117, 1151
OliI CACNNNNGTG 1 cut(s) 665
PaeR7I CTCGAG 1 cut(s) 850
PciSI GCTCTTC 2 cut(s) 233, 1318
PctI GAATGC 1 cut(s) 1337
PfeI GAWTC 2 cut(s) 703, 948
PflFI GACNNNGTC 1 cut(s) 548
PflMI CCANNNNNTGG 1 cut(s) 944
PfoI TCCNGGA 2 cut(s) 87, 574
PinAI ACCGGT 1 cut(s) 257
PkrI GCNGC 3 cut(s) 370, 1268, 1299
PleI GAGTC 2 cut(s) 145, 1035
PpsI GAGTC 2 cut(s) 145, 1035
PpuMI RGGWCCY 1 cut(s) 900
Psp124BI GAGCTC 1 cut(s) 857
Psp1406I AACGTT 2 cut(s) 759, 1256
Psp5II RGGWCCY 1 cut(s) 900
Psp6I CCWGG 3 cut(s) 87, 574, 937
PspGI CCWGG 3 cut(s) 87, 574, 937
PspN4I GGNNCC 6 cut(s) 405, 484, 580, 901, 902, 1030
PspPI GGNCC 4 cut(s) 446, 572, 900, 935
PspPPI RGGWCCY 1 cut(s) 900
PstI CTGCAG 3 cut(s) 474, 1268, 1317
PsyI GACNNNGTC 1 cut(s) 548
PvuII CAGCTG 1 cut(s) 665
RsaI GTAC 4 cut(s) 61, 405, 649, 1214
RsaNI GTAC 4 cut(s) 60, 404, 648, 1213
RseI CAYNNNNRTG 2 cut(s) 126, 665
SacI GAGCTC 1 cut(s) 857
SalI GTCGAC 1 cut(s) 302
SapI GCTCTTC 2 cut(s) 233, 1318
SaqAI TTAA 3 cut(s) 8, 191, 1381
SatI GCNGC 3 cut(s) 369, 1267, 1298
Sau3AI GATC 5 cut(s) 207, 506, 985, 1047, 1290
Sau96I GGNCC 4 cut(s) 446, 572, 900, 935
SchI GAGTC 2 cut(s) 146, 1035
ScrFI CCNGG 3 cut(s) 89, 576, 939
SduI GDGCHC 1 cut(s) 857
SfaNI GCATC 2 cut(s) 503, 762
SfcI CTRYAG 3 cut(s) 470, 1264, 1313
Sfr274I CTCGAG 1 cut(s) 850
SgrAI CRCCGGYG 1 cut(s) 257
SgrDI CGTCGACG 1 cut(s) 302
SinI GGWCC 3 cut(s) 572, 900, 935
SlaI CTCGAG 1 cut(s) 850
SmiMI CAYNNNNRTG 2 cut(s) 126, 665
SmlI CTYRAG 1 cut(s) 850
SmoI CTYRAG 1 cut(s) 850
Sse9I AATT 4 cut(s) 148, 203, 437, 816
SsiI CCGC 2 cut(s) 645, 1190
SspMI CTAG 3 cut(s) 153, 563, 1374
SstI GAGCTC 1 cut(s) 857
StyD4I CCNGG 3 cut(s) 87, 574, 937
StyI CCWWGG 1 cut(s) 943
TaaI ACNGT 4 cut(s) 18, 781, 881, 1151
TaiI ACGT 4 cut(s) 117, 762, 1058, 1259
TaqI TCGA 3 cut(s) 303, 851, 1137
TaqII GACCGA 1 cut(s) 519
TasI AATT 4 cut(s) 148, 203, 437, 816
TatI WGTACW 1 cut(s) 59
TfiI GAWTC 2 cut(s) 703, 948
Tru1I TTAA 3 cut(s) 8, 191, 1381
Tru9I TTAA 3 cut(s) 8, 191, 1381
TscAI CASTG 4 cut(s) 388, 474, 536, 1081
TseFI GTSAC 4 cut(s) 667, 823, 1117, 1151
TseI GCWGC 3 cut(s) 368, 1266, 1297
Tsp45I GTSAC 4 cut(s) 667, 823, 1117, 1151
TspDTI ATGAA 6 cut(s) 57, 95, 156, 187, 809, 1124
TspGWI ACGGA 4 cut(s) 188, 424, 824, 828
TspRI CASTG 4 cut(s) 388, 474, 536, 1081
Tth111I GACNNNGTC 1 cut(s) 548
Van91I CCANNNNNTGG 1 cut(s) 944
VpaK11BI GGWCC 3 cut(s) 572, 900, 935
XagI CCTNNNNNAGG 1 cut(s) 676
XapI RAATTY 2 cut(s) 437, 816
XcmI CCANNNNNNNNNTGG 1 cut(s) 388
XhoI CTCGAG 1 cut(s) 850
XmiI GTMKAC 1 cut(s) 303
XspI CTAG 3 cut(s) 153, 563, 1374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.