pycom10g15530

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003318
Physical Location & Seq
Forward (+)
18777450 .. 18779846
2397 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g15530.3

Sequence Viewer

Length: 1383 bp
ATGGCTTTAAAAACACAGTTGTTGTGGTCATTTGTTGTTGTTTTTGTTGTTTCCTTCAGTACAACTTCATGTTCTGGTAGTAGTTTCCAGGAGGTCAACGCGCTTCATAGTTACGTTGACCATGTTGATGATAGGGTGTCCGGCTACAATTCTAGGGCTTATCCTTCATACATGGACACCATTGAAGGTTTAAAGTTCATGGAATTGATCAGGCCAAGAACTCAGCTCTCCAGTTCAAGGAAGCTCAACACAATCACCGGTGGGATAGCAACATCATCAGCTCCGGCCAAAACCATTAGCGTCGACGATTTTGGAGCTAAAGGGAATGGTGCTGATGACACACAGGCATTTGTGAAGGCATGGAAGGCAGCTTGTTCTTCCAGTGGAGCAATAGTTCTTGTGGTACCACAGAAGAAATATCTTGTTAGGCCAATTGATTTCTCAGGCCCATGCAAATCTCAACTTACAGTGCAGATTTATGGAACCATAGAAGCATCAGAAGACCGATCAATCTACAAAGACATAGACCACTGGCTCATCTTTGACAATGTCCAAAACTTGCTAGTTGTTGGTCCTGGAACCATCAATGGCAGTGGAAACATCTGGTGGAAAAACTCATGCAAAAGAAAACCTCAGCCCCCTTGCGGTACACACGCCCCCACGGCTGTGACCTTCAACAGGTGCAATAACTTGGTGGTGAAGAATCTGAAGATCCAAGACGCACAACAAATGCATGTCAGATTCCAAAACTGCATCAACGTCCAAGCTTCCCGTCTCACGGTAACCGCACCGGAGGATAGCCCTAATACGGACGGAATTCATGTGACAAATACCCAGAACATCACTATCTCGAGCTCGGTTATAGGAACAGGTGATGACTGTATTTCTATTGTAAGTGGGTCCCAAAGAGTTCAAGCCACAGACATTACTTGTGGGCCAGGCCATGGAATCAGTATTGGTAGCTTGGGAGAAGACGGCTCAAAAGATCATGTTTCAGGAGTGTGTGTGAATGGAGCTAAGCTTTCAGGAACCTCCAATGGACTCCGGATCAAGACGTGGCAGGGAGGCTCAGGCAGTGCAACCAACATTGTTTTCCAGAATGTGCAAATGAACAATGTCACCAACCCCATCATCATCGACCAGAACTACTGTGACCACAAAACCAAAGATTGCAAACAACAGAAATCGGCGGTTCAAGTGAAAAATGTGTTATACCAAAACATAAGAGGAACGAGTGCTTCCGGCGACGCGATAACGTTGAACTGCAGCCAAAGTGTTCCTTGTCGGGGGATCGCGCTGCAAAGTGTTCGACTGCAGAATGGAAGAGCTGAATGCAACAATGTTCAGCCTGCTTACAAAGGAGTTGCCTCCCCTAGATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

461

Amino Acids

49.46

Weight (kDa)

8.85

Isoelectric Point (pI)

32.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 403
AccB1I GGYRCC 1 cut(s) 403
AccB7I CCANNNNNTGG 1 cut(s) 944
AccI GTMKAC 1 cut(s) 303
AccII CGCG 3 cut(s) 101, 1250, 1295
AccIII TCCGGA 1 cut(s) 1044
AciI CCGC 3 cut(s) 645, 786, 1190
AclI AACGTT 1 cut(s) 1256
AclWI GGATC 3 cut(s) 706, 1055, 1298
AcoI YGGCCR 1 cut(s) 285
AcsI RAATTY 1 cut(s) 816
AcuI CTGAAG 2 cut(s) 40, 728
AfaI GTAC 3 cut(s) 61, 405, 649
AfiI CCNNNNNNNGG 7 cut(s) 237, 644, 678, 778, 808, 944, 1286
AgeI ACCGGT 1 cut(s) 257
AgsI TTSAA 6 cut(s) 185, 237, 676, 914, 1196, 1261
AjiI CACGTC 1 cut(s) 1056
AjnI CCWGG 3 cut(s) 87, 574, 937
AjuI GAANNNNNNNTTGG 2 cut(s) 1076, 1108
AleI CACNNNNGTG 1 cut(s) 665
AloI GAACNNNNNNTCC 2 cut(s) 378, 410
Alw21I GWGCWC 1 cut(s) 857
Alw26I GTCTC 1 cut(s) 779
AlwI GGATC 3 cut(s) 706, 1055, 1298
Ama87I CYCGRG 1 cut(s) 850
Aor13HI TCCGGA 1 cut(s) 1044
AoxI GGCC 6 cut(s) 212, 285, 428, 445, 935, 940
ApeKI GCWGC 3 cut(s) 368, 1266, 1297
ApoI RAATTY 1 cut(s) 816
AsiGI ACCGGT 1 cut(s) 257
Asp718I GGTACC 1 cut(s) 403
AspLEI GCGC 2 cut(s) 103, 1297
AspS9I GGNCC 4 cut(s) 446, 572, 900, 935
AsuHPI GGTGA 4 cut(s) 247, 709, 884, 1111
AvaI CYCGRG 1 cut(s) 850
AvaII GGWCC 2 cut(s) 572, 900
BanI GGYRCC 1 cut(s) 403
BanII GRGCYC 1 cut(s) 857
BbsI GAAGAC 2 cut(s) 507, 978
Bbv12I GWGCWC 1 cut(s) 857
BbvCI CCTCAGC 1 cut(s) 633
BbvI GCAGC 3 cut(s) 380, 1278, 1284
BccI CCATC 2 cut(s) 590, 1136
BceAI ACGGC 2 cut(s) 678, 991
BcgI CGANNNNNNTGC 2 cut(s) 1289, 1323
BciT130I CCWGG 3 cut(s) 89, 576, 939
BclI TGATCA 1 cut(s) 207
BcoDI GTCTC 1 cut(s) 779
BfaI CTAG 3 cut(s) 153, 563, 1374
BfmI CTRYAG 2 cut(s) 1264, 1313
BglI GCCNNNNNGGC 1 cut(s) 662
BisI GCNGC 3 cut(s) 369, 1267, 1298
BlpI GCTNAGC 1 cut(s) 1017
BlsI GCNGC 3 cut(s) 370, 1268, 1299
Bme1390I CCNGG 3 cut(s) 89, 576, 939
Bme18I GGWCC 2 cut(s) 572, 900
BmeT110I CYCGRG 1 cut(s) 850
BmgBI CACGTC 1 cut(s) 1056
BmgT120I GGNCC 4 cut(s) 446, 572, 900, 935
BmiI GGNNCC 6 cut(s) 405, 484, 580, 901, 902, 1030
BmrFI CCNGG 3 cut(s) 89, 576, 939
BmsI GCATC 2 cut(s) 503, 762
BpiI GAAGAC 2 cut(s) 507, 978
BpmI CTGGAG 1 cut(s) 214
Bpu10I CCTNAGC 2 cut(s) 633, 1069
Bpu1102I GCTNAGC 1 cut(s) 1017
BsaJI CCNNGG 2 cut(s) 660, 943
BsaWI WCCGGW 3 cut(s) 257, 790, 1044
BsaXI ACNNNNNCTCC 4 cut(s) 378, 408, 990, 1020
Bsc4I CCNNNNNNNGG 7 cut(s) 237, 644, 678, 778, 808, 944, 1286
Bse118I RCCGGY 1 cut(s) 257
Bse1I ACTGG 3 cut(s) 231, 381, 536
BseAI TCCGGA 1 cut(s) 1044
BseBI CCWGG 3 cut(s) 89, 576, 939
BseDI CCNNGG 2 cut(s) 660, 943
BseLI CCNNNNNNNGG 7 cut(s) 237, 644, 678, 778, 808, 944, 1286
BseMII CTCAG 4 cut(s) 236, 456, 647, 1083
BseNI ACTGG 3 cut(s) 231, 381, 536
BseXI GCAGC 3 cut(s) 380, 1278, 1284
BsgI GTGCAG 1 cut(s) 491
Bsh1236I CGCG 3 cut(s) 101, 1250, 1295
BshFI GGCC 6 cut(s) 214, 287, 430, 447, 937, 942
BshNI GGYRCC 1 cut(s) 403
BshTI ACCGGT 1 cut(s) 257
BsiHKAI GWGCWC 1 cut(s) 857
BsiHKCI CYCGRG 1 cut(s) 850
BsiSI CCGG 6 cut(s) 141, 258, 284, 791, 1045, 1242
BslFI GGGAC 1 cut(s) 886
BslI CCNNNNNNNGG 7 cut(s) 237, 644, 678, 778, 808, 944, 1286
BsmAI GTCTC 1 cut(s) 779
BsmBI CGTCTC 1 cut(s) 779
BsmFI GGGAC 1 cut(s) 886
BsmI GAATGC 1 cut(s) 1337
BsnI GGCC 6 cut(s) 214, 287, 430, 447, 937, 942
BsoBI CYCGRG 1 cut(s) 850
Bsp1286I GDGCHC 1 cut(s) 857
Bsp13I TCCGGA 1 cut(s) 1044
Bsp143I GATC 6 cut(s) 207, 506, 711, 985, 1047, 1290
Bsp1720I GCTNAGC 1 cut(s) 1017
Bsp19I CCATGG 1 cut(s) 943
BspACI CCGC 3 cut(s) 645, 786, 1190
BspANI GGCC 6 cut(s) 214, 287, 430, 447, 937, 942
BspCNI CTCAG 4 cut(s) 235, 455, 646, 1082
BspEI TCCGGA 1 cut(s) 1044
BspFNI CGCG 3 cut(s) 101, 1250, 1295
BspLI GGNNCC 6 cut(s) 405, 484, 580, 901, 902, 1030
BspMAI CTGCAG 2 cut(s) 1268, 1317
BspPI GGATC 3 cut(s) 706, 1055, 1298
BspQI GCTCTTC 1 cut(s) 1318
BspT107I GGYRCC 1 cut(s) 403
BsrFI RCCGGY 1 cut(s) 257
BsrI ACTGG 3 cut(s) 231, 381, 536
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 2 cut(s) 660, 943
BssMI GATC 6 cut(s) 207, 506, 711, 985, 1047, 1290
BssT1I CCWWGG 1 cut(s) 943
Bst2UI CCWGG 3 cut(s) 89, 576, 939
Bst4CI ACNGT 5 cut(s) 18, 469, 781, 881, 1151
Bst6I CTCTTC 1 cut(s) 1318
BstC8I GCNNGC 1 cut(s) 1350
BstDEI CTNAG 5 cut(s) 222, 442, 633, 1017, 1069
BstDSI CCRYGG 2 cut(s) 660, 943
BstEII GGTNACC 1 cut(s) 781
BstENI CCTNNNNNAGG 1 cut(s) 676
BstFNI CGCG 3 cut(s) 101, 1250, 1295
BstHHI GCGC 2 cut(s) 103, 1297
BstKTI GATC 6 cut(s) 210, 509, 714, 988, 1050, 1293
BstMAI GTCTC 1 cut(s) 779
BstMBI GATC 6 cut(s) 207, 506, 711, 985, 1047, 1290
BstMWI GCNNNNNNNGC 2 cut(s) 365, 662
BstNI CCWGG 3 cut(s) 89, 576, 939
BstNSI RCATGY 1 cut(s) 737
BstPI GGTNACC 1 cut(s) 781
BstSCI CCNGG 3 cut(s) 87, 574, 937
BstSFI CTRYAG 2 cut(s) 1264, 1313
BstUI CGCG 3 cut(s) 101, 1250, 1295
BstV1I GCAGC 3 cut(s) 380, 1278, 1284
BstV2I GAAGAC 2 cut(s) 507, 978
BstX2I RGATCY 1 cut(s) 711
BstYI RGATCY 1 cut(s) 711
BsuRI GGCC 6 cut(s) 214, 287, 430, 447, 937, 942
BtgI CCRYGG 2 cut(s) 660, 943
BtrI CACGTC 1 cut(s) 1056
BtsI GCAGTG 2 cut(s) 598, 1081
BtsIMutI CAGTG 5 cut(s) 388, 474, 529, 598, 1081
Cac8I GCNNGC 1 cut(s) 1350
CfoI GCGC 2 cut(s) 103, 1297
Cfr10I RCCGGY 1 cut(s) 257
Cfr13I GGNCC 4 cut(s) 446, 572, 900, 935
CseI GACGC 3 cut(s) 289, 728, 1256
Csp6I GTAC 3 cut(s) 60, 404, 648
CspAI ACCGGT 1 cut(s) 257
CspCI CAANNNNNGTGG 2 cut(s) 574, 609
CviQI GTAC 3 cut(s) 60, 404, 648
DdeI CTNAG 5 cut(s) 222, 442, 633, 1017, 1069
DpnI GATC 6 cut(s) 209, 508, 713, 987, 1049, 1292
DpnII GATC 6 cut(s) 207, 506, 711, 985, 1047, 1290
DraI TTTAAA 2 cut(s) 9, 192
EaeI YGGCCR 1 cut(s) 285
Eam1104I CTCTTC 1 cut(s) 1318
EarI CTCTTC 1 cut(s) 1318
Ecl136II GAGCTC 1 cut(s) 855
Eco130I CCWWGG 1 cut(s) 943
Eco24I GRGCYC 1 cut(s) 857
Eco47I GGWCC 2 cut(s) 572, 900
Eco53kI GAGCTC 1 cut(s) 855
Eco57I CTGAAG 2 cut(s) 40, 728
Eco88I CYCGRG 1 cut(s) 850
Eco91I GGTNACC 1 cut(s) 781
EcoICRI GAGCTC 1 cut(s) 855
EcoNI CCTNNNNNAGG 1 cut(s) 676
EcoO109I RGGNCCY 1 cut(s) 900
EcoO65I GGTNACC 1 cut(s) 781
EcoRI GAATTC 1 cut(s) 816
EcoRII CCWGG 3 cut(s) 87, 574, 937
EcoT14I CCWWGG 1 cut(s) 943
EcoT22I ATGCAT 1 cut(s) 735
EcoT38I GRGCYC 1 cut(s) 857
ErhI CCWWGG 1 cut(s) 943
Esp3I CGTCTC 1 cut(s) 779
FalI AAGNNNNNCTT 2 cut(s) 1264, 1296
FaqI GGGAC 1 cut(s) 886
FbaI TGATCA 1 cut(s) 207
FblI GTMKAC 1 cut(s) 303
Fnu4HI GCNGC 3 cut(s) 369, 1267, 1298
FriOI GRGCYC 1 cut(s) 857
Fsp4HI GCNGC 3 cut(s) 369, 1267, 1298
FspBI CTAG 3 cut(s) 153, 563, 1374
GlaI GCGC 2 cut(s) 102, 1296
GluI GCNGC 3 cut(s) 369, 1267, 1298
GsuI CTGGAG 1 cut(s) 214
HaeIII GGCC 6 cut(s) 214, 287, 430, 447, 937, 942
HapII CCGG 6 cut(s) 141, 258, 284, 791, 1045, 1242
HgaI GACGC 3 cut(s) 289, 728, 1256
HhaI GCGC 2 cut(s) 103, 1297
Hin6I GCGC 2 cut(s) 101, 1295
HinP1I GCGC 2 cut(s) 101, 1295
HincII GTYRAC 3 cut(s) 97, 118, 304
HindII GTYRAC 3 cut(s) 97, 118, 304
HindIII AAGCTT 2 cut(s) 765, 1019
HinfI GANTC 4 cut(s) 703, 741, 948, 1041
HpaII CCGG 6 cut(s) 141, 258, 284, 791, 1045, 1242
HphI GGTGA 4 cut(s) 247, 709, 884, 1111
Hpy166II GTNNAC 4 cut(s) 97, 118, 304, 650
Hpy188I TCNGA 3 cut(s) 499, 708, 740
Hpy188III TCNNGA 6 cut(s) 850, 996, 1026, 1045, 1051, 1096
Hpy8I GTNNAC 4 cut(s) 97, 118, 304, 650
Hpy99I CGWCG 3 cut(s) 305, 308, 1250
HpyAV CCTTC 6 cut(s) 64, 174, 179, 349, 358, 682
HpyCH4III ACNGT 5 cut(s) 18, 469, 781, 881, 1151
HpyCH4IV ACGT 4 cut(s) 114, 759, 1055, 1256
HpyF10VI GCNNNNNNNGC 2 cut(s) 365, 662
HpyF3I CTNAG 5 cut(s) 222, 442, 633, 1017, 1069
HpySE526I ACGT 4 cut(s) 114, 759, 1055, 1256
HspAI GCGC 2 cut(s) 101, 1295
KflI GGGWCCC 1 cut(s) 900
Kpn2I TCCGGA 1 cut(s) 1044
KpnI GGTACC 1 cut(s) 407
Ksp22I TGATCA 1 cut(s) 207
Kzo9I GATC 6 cut(s) 207, 506, 711, 985, 1047, 1290
LguI GCTCTTC 1 cut(s) 1318
LmnI GCTCC 4 cut(s) 286, 314, 386, 1013
Lsp1109I GCAGC 3 cut(s) 380, 1278, 1284
LweI GCATC 2 cut(s) 503, 762
MaeI CTAG 3 cut(s) 153, 563, 1374
MaeII ACGT 4 cut(s) 114, 759, 1055, 1256
MaeIII GTNAC 6 cut(s) 110, 667, 781, 823, 1117, 1151
MalI GATC 6 cut(s) 209, 508, 713, 987, 1049, 1292
MboI GATC 6 cut(s) 207, 506, 711, 985, 1047, 1290
MboII GAAGA 7 cut(s) 369, 424, 512, 712, 721, 983, 1335
MfeI CAATTG 1 cut(s) 432
MflI RGATCY 1 cut(s) 711
MhlI GDGCHC 1 cut(s) 857
MluCI AATT 4 cut(s) 148, 203, 432, 816
MlyI GAGTC 1 cut(s) 1035
MnlI CCTC 7 cut(s) 85, 642, 787, 1042, 1058, 1220, 1378
Mph1103I ATGCAT 1 cut(s) 735
MroI TCCGGA 1 cut(s) 1044
MseI TTAA 3 cut(s) 8, 191, 1381
MslI CAYNNNNRTG 2 cut(s) 126, 665
MspI CCGG 6 cut(s) 141, 258, 284, 791, 1045, 1242
MspR9I CCNGG 3 cut(s) 89, 576, 939
MunI CAATTG 1 cut(s) 432
Mva1269I GAATGC 1 cut(s) 1337
MvaI CCWGG 3 cut(s) 89, 576, 939
MvnI CGCG 3 cut(s) 101, 1250, 1295
MwoI GCNNNNNNNGC 2 cut(s) 365, 662
NcoI CCATGG 1 cut(s) 943
NdeII GATC 6 cut(s) 207, 506, 711, 985, 1047, 1290
NlaIV GGNNCC 6 cut(s) 405, 484, 580, 901, 902, 1030
NmuCI GTSAC 4 cut(s) 667, 823, 1117, 1151
NsiI ATGCAT 1 cut(s) 735
NspI RCATGY 1 cut(s) 737
OliI CACNNNNGTG 1 cut(s) 665
PaeR7I CTCGAG 1 cut(s) 850
PciSI GCTCTTC 1 cut(s) 1318
PctI GAATGC 1 cut(s) 1337
PfeI GAWTC 3 cut(s) 703, 741, 948
PflFI GACNNNGTC 1 cut(s) 548
PflMI CCANNNNNTGG 1 cut(s) 944
PfoI TCCNGGA 2 cut(s) 87, 574
PinAI ACCGGT 1 cut(s) 257
PkrI GCNGC 3 cut(s) 370, 1268, 1299
PleI GAGTC 1 cut(s) 1035
PpsI GAGTC 1 cut(s) 1035
PpuMI RGGWCCY 1 cut(s) 900
Psp124BI GAGCTC 1 cut(s) 857
Psp1406I AACGTT 1 cut(s) 1256
Psp5II RGGWCCY 1 cut(s) 900
Psp6I CCWGG 3 cut(s) 87, 574, 937
PspEI GGTNACC 1 cut(s) 781
PspGI CCWGG 3 cut(s) 87, 574, 937
PspN4I GGNNCC 6 cut(s) 405, 484, 580, 901, 902, 1030
PspPI GGNCC 4 cut(s) 446, 572, 900, 935
PspPPI RGGWCCY 1 cut(s) 900
PstI CTGCAG 2 cut(s) 1268, 1317
PsuI RGATCY 1 cut(s) 711
PsyI GACNNNGTC 1 cut(s) 548
RsaI GTAC 3 cut(s) 61, 405, 649
RsaNI GTAC 3 cut(s) 60, 404, 648
RseI CAYNNNNRTG 2 cut(s) 126, 665
SacI GAGCTC 1 cut(s) 857
SalI GTCGAC 1 cut(s) 302
SapI GCTCTTC 1 cut(s) 1318
SaqAI TTAA 3 cut(s) 8, 191, 1381
SatI GCNGC 3 cut(s) 369, 1267, 1298
Sau3AI GATC 6 cut(s) 207, 506, 711, 985, 1047, 1290
Sau96I GGNCC 4 cut(s) 446, 572, 900, 935
SchI GAGTC 1 cut(s) 1035
ScrFI CCNGG 3 cut(s) 89, 576, 939
SduI GDGCHC 1 cut(s) 857
SfaNI GCATC 2 cut(s) 503, 762
SfcI CTRYAG 2 cut(s) 1264, 1313
Sfr274I CTCGAG 1 cut(s) 850
SgrAI CRCCGGYG 1 cut(s) 257
SgrDI CGTCGACG 1 cut(s) 302
SinI GGWCC 2 cut(s) 572, 900
SlaI CTCGAG 1 cut(s) 850
SmiMI CAYNNNNRTG 2 cut(s) 126, 665
SmlI CTYRAG 1 cut(s) 850
SmoI CTYRAG 1 cut(s) 850
Sse9I AATT 4 cut(s) 148, 203, 432, 816
SsiI CCGC 3 cut(s) 645, 786, 1190
SspMI CTAG 3 cut(s) 153, 563, 1374
SstI GAGCTC 1 cut(s) 857
StyD4I CCNGG 3 cut(s) 87, 574, 937
StyI CCWWGG 1 cut(s) 943
TaaI ACNGT 5 cut(s) 18, 469, 781, 881, 1151
TaiI ACGT 4 cut(s) 117, 762, 1058, 1259
TaqI TCGA 4 cut(s) 303, 851, 1137, 1309
TaqII GACCGA 1 cut(s) 519
TasI AATT 4 cut(s) 148, 203, 432, 816
TatI WGTACW 1 cut(s) 59
TfiI GAWTC 3 cut(s) 703, 741, 948
Tru1I TTAA 3 cut(s) 8, 191, 1381
Tru9I TTAA 3 cut(s) 8, 191, 1381
TscAI CASTG 5 cut(s) 388, 474, 536, 598, 1081
TseFI GTSAC 4 cut(s) 667, 823, 1117, 1151
TseI GCWGC 3 cut(s) 368, 1266, 1297
Tsp45I GTSAC 4 cut(s) 667, 823, 1117, 1151
TspDTI ATGAA 6 cut(s) 57, 95, 156, 187, 809, 1124
TspGWI ACGGA 2 cut(s) 824, 828
TspRI CASTG 5 cut(s) 388, 474, 536, 598, 1081
Tth111I GACNNNGTC 1 cut(s) 548
Van91I CCANNNNNTGG 1 cut(s) 944
VpaK11BI GGWCC 2 cut(s) 572, 900
XagI CCTNNNNNAGG 1 cut(s) 676
XapI RAATTY 1 cut(s) 816
XceI RCATGY 1 cut(s) 737
XhoI CTCGAG 1 cut(s) 850
XmiI GTMKAC 1 cut(s) 303
XspI CTAG 3 cut(s) 153, 563, 1374
Zsp2I ATGCAT 1 cut(s) 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.