Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003318

Physical Location & Seq

Genomic Coordinates Reverse (-)

9428552 .. 9433655

Gene Structure 5104 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1077600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1389 bp

ATGGCTCTTCAGAAAAATGAACTCATCCTATTCTTTATCATCACCCTCCCCTCCTTTGTTTTATGTTATAGCAGTTTACATGAGAACCAACTCATCGACAAGTATGTTGACGAAGCATCTGAATTTGATTCCAAAGCGTATCCATCTTACTTCAACACGATCGACGATGGCAGTAACTTGTTCAAGGGCTTGATAAAGGAAAGTGCAGATGTCCTATGCTTAAAGAGCTTCGATAAAGTGGATAGCAGCACTTCATCTTCGGCTGAGACGGTCAGTGTTAATGATTTTGGAGCCGAGGGTGATGGAGAAACTGATGACACAAAGGCTTTTGAGATGGCTTGGAAGGTAGCTTGTTCATCTAAGGAAGCTGTTATGGTGGCGCCTAAGAAGACGTATCGTATTAAACCAATCAGATTTTCAGGTCCTTGCAAATCTCAACTTACAGTGCAGATTCTGGGAACGTTAATAGCTTCTGATGATCGATCTGACTTCAGCAAAGACGGTAGACACTGGCTTATCTTTGACAGTGTTTCCAATTTGGTGGTTGAAGGTGGTGGGGCCATCAATGGCAATGGGAAAAATTGGTGGCAAAATTCATGCAAAACCGATAAATCGAAGACACTCACCTTCTATAAGAGCAAGAATTTGATGGTGAGGAACCTCACAATTCAAGATGGACAACAAATTCATGTGTCTTTCCAGAAATGCATGAATGTTGAAGTTTCCAATCTCTCAATAACTGCACCACCAAAAAGCCCCAACACCGACGGAATTCATGTTACAGATACTCAAAACATATTGATCACAAGCTGTGTTATAGCCACAGGTGATGATTGTCTTTCTATTGTAAATGGATCCAAAACGGTGCAAGCTTCGAACATAACCTGCGGATCAGGCCATGGTATCAGTATTGGAAGCTTGGGATCTGGAAACTCAAAAGCGTATGTTTCGCATATTGCAGTGAATGGAGCAAAACTTTATGGAACTACAAATGGAATTCGGATCAAAACGTGGCAGGGAGGATCAGGAAGTGCAAGCAACATCAGATTTCAGAACATTGAAATGCATAATGTTACCAACCCCATACTTATAGACCAATATTACTGTGACAGAAAGATACCGTGCAAAGAACAGAGATCAGCAGTTGAAGTCAAAAATGTGGTGTACAAAAACATCAAAGGAACAAGTGCTTCAGATGTTGCTATAAAATTCGATTGCAGCAAGAGCTTCCCTTGTCTGGGGATTATATTGCAAGATATTAACCTCCAACACGAAGGAAGGAAAACAGCTAAAGCTCTGTGCAACAATGTTAATGTGACTAGCATAGGGGTTGTTTGCCCGCTTTGTCCTAAATTAGAAGGGCAATATGAGATTTGCCAATGTCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0004650 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0007275 GO:0008150 GO:0009664 GO:0009791 GO:0009827 GO:0009830 GO:0009838 GO:0009900 GO:0009901 GO:0009908 GO:0009987 GO:0010047 GO:0010227 GO:0016043 GO:0016787 GO:0016798 GO:0022411 GO:0022414 GO:0030312 GO:0032501 GO:0032502 GO:0032504 GO:0042545 GO:0044277 GO:0044464 GO:0045229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048608 GO:0048609 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0071944 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

Glyco_hydro_28

Protein Analysis

463

Amino Acids

50.33

Weight (kDa)

7.42

Isoelectric Point (pI)

31.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pect-lyase_RHGA_epim	PF12708	91 - 310	8.1e-07	Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28	PF00295	122 - 440	5.5e-97	Glycosyl hydrolases family 28

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003318)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41850
fragaria_vesca	FvH4_2g20930 FvH4_2g20930 FvH4_6g23580
malus_domestica	MD05G1192600.v1.1 MD10G1179100.v1.1 MD12G1077400.v1.1 MD12G1077600.v1.1
prunus_persica	Prupe.7G063900_v2.0.a1 Prupe.8G218900_v2.0.a1
pyrus_communis	pycom05g17960 pycom10g15530 pycom12g06230
rosa_chinensis	RchiOBHm_Chr3g0481691 RchiOBHm_Chr6g0287311
rosa_laevigata	RLG00000012502 RLG00000023383
rosa_multiflora	Rmu_sc0007029.1_g000032 Rmu_ssc0000267.1_g000001
rosa_roxburghii	Rroxscaffold_6G00399870 Rroxscaffold_6G00400230 Rroxscaffold_7G00181550
rosa_rugosa	Rorug03G0193400 Rorug06G0186800 Rorug06G0186900
rosa_samantha	Rh3AG244500 Rh3BG279600 Rh3CG274700 Rh3DG271500 Rh6AG298300 Rh6BG303700 Rh6CG306100 Rh6CG312000 Rh6DG295600
rosa_wichuraiana	Rw3G021820 Rw6G025780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	893
AccB1I	GGYRCC	1 cut(s)	379
AccI	GTMKAC	1 cut(s)	505
AciI	CCGC	2 cut(s)	888, 1340
AclI	AACGTT	1 cut(s)	461
AclWI	GGATC	6 cut(s)	849, 862, 898, 931, 1010, 1030
AcsI	RAATTY	7 cut(s)	122, 592, 643, 684, 771, 996, 1208
AcuI	CTGAAG	2 cut(s)	475, 1176
AcyI	GRCGYC	1 cut(s)	380
AfaI	GTAC	1 cut(s)	1166
AfiI	CCNNNNNNNGG	2 cut(s)	1237, 1238
AgsI	TTSAA	7 cut(s)	154, 184, 548, 671, 719, 1061, 1148
AjuI	GAANNNNNNNTTGG	2 cut(s)	400, 432
Alw26I	GTCTC	1 cut(s)	260
AlwI	GGATC	6 cut(s)	849, 862, 898, 931, 1010, 1030
AlwNI	CAGNNNCTG	1 cut(s)	454
AoxI	GGCC	2 cut(s)	558, 895
ApeKI	GCWGC	2 cut(s)	246, 1218
ApoI	RAATTY	7 cut(s)	122, 592, 643, 684, 771, 996, 1208
AspLEI	GCGC	1 cut(s)	382
AspS9I	GGNCC	2 cut(s)	422, 558
AsuHPI	GGTGA	5 cut(s)	34, 311, 616, 664, 839
AsuII	TTCGAA	1 cut(s)	875
AvaII	GGWCC	1 cut(s)	422
BamHI	GGATCC	1 cut(s)	854
BanI	GGYRCC	1 cut(s)	379
BbsI	GAAGAC	2 cut(s)	395, 623
BbvI	GCAGC	2 cut(s)	258, 1230
BccI	CCATC	7 cut(s)	151, 161, 296, 328, 569, 643, 668
BciVI	GTATCC	1 cut(s)	150
BclI	TGATCA	1 cut(s)	801
BcoDI	GTCTC	1 cut(s)	260
BfaI	CTAG	1 cut(s)	1320
BfoI	RGCGCY	1 cut(s)	383
BfuAI	ACCTGC	1 cut(s)	893
BfuI	GTATCC	1 cut(s)	150
BisI	GCNGC	2 cut(s)	247, 1219
BlsI	GCNGC	2 cut(s)	248, 1220
Bme18I	GGWCC	1 cut(s)	422
BmgT120I	GGNCC	2 cut(s)	422, 558
BmiI	GGNNCC	5 cut(s)	292, 381, 559, 659, 856
BmsI	GCATC	1 cut(s)	125
BpiI	GAAGAC	2 cut(s)	395, 623
Bpu14I	TTCGAA	1 cut(s)	875
Bsa29I	ATCGAT	1 cut(s)	481
BsaHI	GRCGYC	1 cut(s)	380
BsaJI	CCNNGG	2 cut(s)	294, 898
Bsc4I	CCNNNNNNNGG	2 cut(s)	1237, 1238
Bse1I	ACTGG	1 cut(s)	515
Bse3DI	GCAATG	1 cut(s)	577
BseCI	ATCGAT	1 cut(s)	481
BseDI	CCNNGG	2 cut(s)	294, 898
BseGI	GGATG	1 cut(s)	24
BseLI	CCNNNNNNNGG	2 cut(s)	1237, 1238
BseMI	GCAATG	1 cut(s)	577
BseMII	CTCAG	1 cut(s)	255
BseNI	ACTGG	1 cut(s)	515
BseXI	GCAGC	2 cut(s)	258, 1230
BsgI	GTGCAG	3 cut(s)	225, 467, 726
Bsh1285I	CGRYCG	1 cut(s)	162
BshFI	GGCC	2 cut(s)	560, 897
BshNI	GGYRCC	1 cut(s)	379
BshVI	ATCGAT	1 cut(s)	481
BsiEI	CGRYCG	1 cut(s)	162
BslI	CCNNNNNNNGG	2 cut(s)	1237, 1238
BsmAI	GTCTC	1 cut(s)	260
BsmBI	CGTCTC	1 cut(s)	260
BsnI	GGCC	2 cut(s)	560, 897
Bsp119I	TTCGAA	1 cut(s)	875
Bsp1407I	TGTACA	1 cut(s)	1164
Bsp19I	CCATGG	1 cut(s)	898
BspACI	CCGC	2 cut(s)	888, 1340
BspANI	GGCC	2 cut(s)	560, 897
BspCNI	CTCAG	1 cut(s)	256
BspDI	ATCGAT	1 cut(s)	481
BspLI	GGNNCC	5 cut(s)	292, 381, 559, 659, 856
BspMI	ACCTGC	1 cut(s)	893
BspPI	GGATC	6 cut(s)	849, 862, 898, 931, 1010, 1030
BspQI	GCTCTTC	1 cut(s)	12
BspT104I	TTCGAA	1 cut(s)	875
BspT107I	GGYRCC	1 cut(s)	379
BsrDI	GCAATG	1 cut(s)	577
BsrGI	TGTACA	1 cut(s)	1164
BsrI	ACTGG	1 cut(s)	515
BssECI	CCNNGG	2 cut(s)	294, 898
BssNI	GRCGYC	1 cut(s)	380
BssT1I	CCWWGG	1 cut(s)	898
Bst4CI	ACNGT	7 cut(s)	271, 445, 503, 527, 865, 1106, 1122
Bst6I	CTCTTC	1 cut(s)	12
BstACI	GRCGYC	1 cut(s)	380
BstAUI	TGTACA	1 cut(s)	1164
BstBI	TTCGAA	1 cut(s)	875
BstC8I	GCNNGC	3 cut(s)	870, 1036, 1340
BstDEI	CTNAG	3 cut(s)	264, 360, 384
BstDSI	CCRYGG	1 cut(s)	898
BstF5I	GGATG	1 cut(s)	24
BstH2I	RGCGCY	1 cut(s)	383
BstHHI	GCGC	1 cut(s)	382
BstMAI	GTCTC	1 cut(s)	260
BstMCI	CGRYCG	1 cut(s)	162
BstMWI	GCNNNNNNNGC	3 cut(s)	225, 894, 1224
BstV1I	GCAGC	2 cut(s)	258, 1230
BstV2I	GAAGAC	2 cut(s)	395, 623
BstX2I	RGATCY	2 cut(s)	854, 923
BstXI	CCANNNNNNTGG	1 cut(s)	541
BstYI	RGATCY	2 cut(s)	854, 923
Bsu15I	ATCGAT	1 cut(s)	481
BsuI	GTATCC	1 cut(s)	150
BsuRI	GGCC	2 cut(s)	560, 897
BsuTUI	ATCGAT	1 cut(s)	481
BtgI	CCRYGG	1 cut(s)	898
BtsCI	GGATG	1 cut(s)	24
BtsI	GCAGTG	1 cut(s)	966
BtsIMutI	CAGTG	5 cut(s)	280, 450, 508, 532, 966
BveI	ACCTGC	1 cut(s)	893
Cac8I	GCNNGC	3 cut(s)	870, 1036, 1340
CaiI	CAGNNNCTG	1 cut(s)	454
CfoI	GCGC	1 cut(s)	382
Cfr13I	GGNCC	2 cut(s)	422, 558
ClaI	ATCGAT	1 cut(s)	481
Csp6I	GTAC	1 cut(s)	1165
CviAII	CATG	6 cut(s)	80, 597, 689, 709, 776, 899
CviQI	GTAC	1 cut(s)	1165
DdeI	CTNAG	3 cut(s)	264, 360, 384
DinI	GGCGCC	1 cut(s)	381
Eam1104I	CTCTTC	1 cut(s)	12
EarI	CTCTTC	1 cut(s)	12
Eco130I	CCWWGG	1 cut(s)	898
Eco47I	GGWCC	1 cut(s)	422
Eco57I	CTGAAG	2 cut(s)	475, 1176
EcoO109I	RGGNCCY	1 cut(s)	422
EcoRI	GAATTC	2 cut(s)	771, 996
EcoT14I	CCWWGG	1 cut(s)	898
EcoT22I	ATGCAT	2 cut(s)	710, 1068
EgeI	GGCGCC	1 cut(s)	381
EheI	GGCGCC	1 cut(s)	381
ErhI	CCWWGG	1 cut(s)	898
Esp3I	CGTCTC	1 cut(s)	260
FaeI	CATG	6 cut(s)	83, 600, 692, 712, 779, 902
FatI	CATG	6 cut(s)	79, 596, 688, 708, 775, 898
FauI	CCCGC	1 cut(s)	1347
FbaI	TGATCA	1 cut(s)	801
FblI	GTMKAC	1 cut(s)	505
Fnu4HI	GCNGC	2 cut(s)	247, 1219
FokI	GGATG	1 cut(s)	11
Fsp4HI	GCNGC	2 cut(s)	247, 1219
FspBI	CTAG	1 cut(s)	1320
GlaI	GCGC	1 cut(s)	381
GluI	GCNGC	2 cut(s)	247, 1219
HaeII	RGCGCY	1 cut(s)	383
HaeIII	GGCC	2 cut(s)	560, 897
HhaI	GCGC	1 cut(s)	382
Hin1I	GRCGYC	1 cut(s)	380
Hin1II	CATG	6 cut(s)	83, 600, 692, 712, 779, 902
Hin6I	GCGC	1 cut(s)	380
HinP1I	GCGC	1 cut(s)	380
HincII	GTYRAC	1 cut(s)	109
HindII	GTYRAC	1 cut(s)	109
HindIII	AAGCTT	2 cut(s)	870, 916
HinfI	GANTC	2 cut(s)	128, 451
HphI	GGTGA	5 cut(s)	34, 311, 616, 664, 839
Hpy166II	GTNNAC	4 cut(s)	77, 109, 506, 1165
Hpy188I	TCNGA	9 cut(s)	12, 121, 413, 475, 487, 1002, 1046, 1053, 1195
Hpy188III	TCNNGA	4 cut(s)	671, 700, 927, 1026
Hpy8I	GTNNAC	4 cut(s)	77, 109, 506, 1165
Hpy99I	CGWCG	2 cut(s)	167, 770
HpyAV	CCTTC	6 cut(s)	337, 542, 637, 1268, 1272, 1352
HpyCH4III	ACNGT	7 cut(s)	271, 445, 503, 527, 865, 1106, 1122
HpyCH4IV	ACGT	3 cut(s)	392, 461, 1010
HpyF10VI	GCNNNNNNNGC	3 cut(s)	225, 894, 1224
HpyF3I	CTNAG	3 cut(s)	264, 360, 384
HpySE526I	ACGT	3 cut(s)	392, 461, 1010
Hsp92I	GRCGYC	1 cut(s)	380
Hsp92II	CATG	6 cut(s)	83, 600, 692, 712, 779, 902
HspAI	GCGC	1 cut(s)	380
KasI	GGCGCC	1 cut(s)	379
Ksp22I	TGATCA	1 cut(s)	801
LguI	GCTCTTC	1 cut(s)	12
LmnI	GCTCC	2 cut(s)	290, 968
Lsp1109I	GCAGC	2 cut(s)	258, 1230
LweI	GCATC	1 cut(s)	125
MaeI	CTAG	1 cut(s)	1320
MaeII	ACGT	3 cut(s)	392, 461, 1010
MaeIII	GTNAC	5 cut(s)	173, 778, 1072, 1106, 1315
MboII	GAAGA	3 cut(s)	249, 400, 628
MflI	RGATCY	2 cut(s)	854, 923
Mly113I	GGCGCC	1 cut(s)	380
MmeI	TCCRAC	1 cut(s)	1291
MnlI	CCTC	7 cut(s)	56, 61, 289, 648, 671, 1013, 1274
Mph1103I	ATGCAT	2 cut(s)	710, 1068
MseI	TTAA	6 cut(s)	221, 279, 402, 464, 1260, 1311
MslI	CAYNNNNRTG	1 cut(s)	1061
MwoI	GCNNNNNNNGC	3 cut(s)	225, 894, 1224
NarI	GGCGCC	1 cut(s)	380
NcoI	CCATGG	1 cut(s)	898
NlaIII	CATG	6 cut(s)	83, 600, 692, 712, 779, 902
NlaIV	GGNNCC	5 cut(s)	292, 381, 559, 659, 856
NmeAIII	GCCGAG	1 cut(s)	319
NmuCI	GTSAC	2 cut(s)	1106, 1315
NsiI	ATGCAT	2 cut(s)	710, 1068
NspV	TTCGAA	1 cut(s)	875
PciSI	GCTCTTC	1 cut(s)	12
PfeI	GAWTC	2 cut(s)	128, 451
PkrI	GCNGC	2 cut(s)	248, 1220
Ple19I	CGATCG	1 cut(s)	162
PluTI	GGCGCC	1 cut(s)	383
PpuMI	RGGWCCY	1 cut(s)	422
Psp1406I	AACGTT	1 cut(s)	461
Psp5II	RGGWCCY	1 cut(s)	422
PspN4I	GGNNCC	5 cut(s)	292, 381, 559, 659, 856
PspPI	GGNCC	2 cut(s)	422, 558
PspPPI	RGGWCCY	1 cut(s)	422
PstNI	CAGNNNCTG	1 cut(s)	454
PsuI	RGATCY	2 cut(s)	854, 923
PvuI	CGATCG	1 cut(s)	162
RsaI	GTAC	1 cut(s)	1166
RsaNI	GTAC	1 cut(s)	1165
RseI	CAYNNNNRTG	1 cut(s)	1061
SapI	GCTCTTC	1 cut(s)	12
SaqAI	TTAA	6 cut(s)	221, 279, 402, 464, 1260, 1311
SatI	GCNGC	2 cut(s)	247, 1219
Sau96I	GGNCC	2 cut(s)	422, 558
SfaNI	GCATC	1 cut(s)	125
SfoI	GGCGCC	1 cut(s)	381
SfuI	TTCGAA	1 cut(s)	875
SinI	GGWCC	1 cut(s)	422
SmiMI	CAYNNNNRTG	1 cut(s)	1061
SsiI	CCGC	2 cut(s)	888, 1340
SspDI	GGCGCC	1 cut(s)	379
SspI	AATATT	1 cut(s)	1100
SspMI	CTAG	1 cut(s)	1320
StyI	CCWWGG	1 cut(s)	898
TaaI	ACNGT	7 cut(s)	271, 445, 503, 527, 865, 1106, 1122
TaiI	ACGT	3 cut(s)	395, 464, 1013
TaqI	TCGA	7 cut(s)	96, 162, 231, 481, 614, 875, 1212
TatI	WGTACW	1 cut(s)	1164
TfiI	GAWTC	2 cut(s)	128, 451
Tru1I	TTAA	6 cut(s)	221, 279, 402, 464, 1260, 1311
Tru9I	TTAA	6 cut(s)	221, 279, 402, 464, 1260, 1311
TscAI	CASTG	5 cut(s)	280, 450, 515, 532, 966
TseFI	GTSAC	2 cut(s)	1106, 1315
TseI	GCWGC	2 cut(s)	246, 1218
Tsp45I	GTSAC	2 cut(s)	1106, 1315
TspDTI	ATGAA	7 cut(s)	33, 243, 345, 585, 677, 725, 764
TspGWI	ACGGA	1 cut(s)	783
TspRI	CASTG	5 cut(s)	280, 450, 515, 532, 966
VpaK11BI	GGWCC	1 cut(s)	422
XapI	RAATTY	7 cut(s)	122, 592, 643, 684, 771, 996, 1208
XmiI	GTMKAC	1 cut(s)	505
XspI	CTAG	1 cut(s)	1320
Zsp2I	ATGCAT	2 cut(s)	710, 1068

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.