Rorug06G0186900

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003318
Physical Location & Seq
Forward (+)
29626969 .. 29634789
7821 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0186900.1

Sequence Viewer

Length: 2166 bp
ATGAATTCCTTTGTGCTTTTTCCGTCCTCTTTGATTCCTTTGCTTTTGATCACTTCCTTTCTGTCGATCAATTTTCAATTAAGTGTGTGCGCCGATGATACAGTGGAATATGCCAACTGCAGCCAACCTATTACCTGCGGGAGCGTCAAAAGCAACATCTCTTACCCCTTTTGGGGAGCGAACCGAGCCGAATACTGTGGCAAATCTGGGTTTGAGGTCACATGCGAAGCCAACGTCCCAATGATCACCATGAGGAATATCAAGTTCAGAATTCTTGACATGAGCAATAGTAGTACTACTACAACAACTCCTACTGTAAAAGTTGCTAGGCAGGATTACTGGGAAGATATCTGTCCCTCGACATATGTTTCCACAACCCTCGACTTCTCTCTCTTCGCTTACTCTTCTGGGCTTCTGAACGTGTCTTTTTGGTACGGATGCAATGCAACCGTAACAGTCCCAGGTAATTCACAAGTCACAGGTAATTCACGTGTTTGCAACTCAAGCGTCACTGCTAATTACCTCACACAGACGGCAGCTAGTAGTCTCCCGTCTGATCCAGTTAGTTCGGGCGCCTGTCAATATGAGGTTTTGGTTCCTGTATCTGAATCCGCTTCTGCGGCTCTGGAGAGCAATAGTGCAGAAATTGAGACTGCGATAGATGGTGGTTTTGAATTGGATGTGCTAAATGCTGATACTGACCTCTGCAACAGTTGCTTGTCATCAGGGGGAGTTTGTGGGCAAAACACTACTGTTGCCAACGAATTCAAGTGCTTTTGCCGGACTGCTTCATCTTCGACAACCACATGTACTGAAAGCTCTTCACTATCTCCAAATCCAGCTCCATCCAATTCAGGAAACGGTAAACACATAGCAACAGGTCTTGCTGTAGGAGGTGCAATCCTTTTTGGCATTTTTGTGGGATTCTACATATATTATGTCATCCAGCGAAAGAAGAAGAAACGTGCAGCAGAAGCTCAGAGCAAAGAGATTCCCACCCCACTTACAAGCAAAAGCACTGTTACTCCCTCGACTAATCTTTCTCAATCTCAAAGCATCCCTTCTTATCCTTCCTTTAACTCAAAATCAAGGTCTGACCTTGGAAAAGCCAGCACTTACTTTGGAGTTCAGGTCTTCAGCTATGCTGAATTAGAGGAAGCCACTGAAAATTTCAACCCTGCTAAAGAACTTGGAGATGGAGGCTTTGGCACCGTTTACTATGGTAAGCTACATGATGGCCGTGTAGTTGCAGTGAAGCGCCTTTATGAAAACAATTTCAAACGGGTGGAGCAGTTTATGAATGAGGTTGAAATCTTAACACGTCTGGAACACCAGAATCTTGTGAAACTCTATGGATGCACCTCAAGACGTAGCCGAGAACTCCTCCTTGTCTATGAATATATTTCTAATGGAACAGTGGCTGACCATCTCCATGGAAAACGAATGGAGTCTGGCTTTCTCAGTTGGCCTGTTAGACTGAGCATTGCCATAGAGACAGCTGATGCACTGGCTTTCCTTCATCGCAATGATGTGATACACCGTGATGTCAAAACCAACAATATACTTCTGGATGATGACTTTTGTGTGAAAGTTGCTGATTTTGGTCTGTCACGGTTGTTCCCCAATGATCAGACCCATGTGTCAACTGCTCCACAAGGGACGCCGGGCTATGTTGATCCCGAGTATTACCAATGCTATCAACTCACTGACAAAAGTGATGTTTATAGCTTTGGGGTCGTCTTGATCGAGCTTATATCATCCTTACAAGCTGTTGACACCAACAGGCACCGGCATGATATAAATTTGGCCAACATGGCTATCAACAAAATTCAAAATCATTTGGTGAATGAGTTGGTTGATCCACTTCTTGGGTATGACAAGGACTATTCTGTGAGGATAATGGCAACAGCTGTTGCAGAATTGGCCTTCCGGTGTTTGCAGCAGGAGAAAGATATGAGACCAACCATGGAAGAAGTACTGGAGGGTTTAAAAGCAATCCAAAATGAAGACCTTGGTGCACAAGAAAATGGGGAGGCAGAGGCAGTGGTCCTGGATATAGGAGCTGATGAAGTTGGCCTTTTGAGGAATTTGCCTCCCCCACTCTCACCAGACTCGGTTGTCACTGATAAATTGGTTAGCAGCTCTCCCACACCTAGTTCCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

721

Amino Acids

78.7

Weight (kDa)

4.96

Isoelectric Point (pI)

39.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 40 - 100 3.7e-13 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 176 - 261 1.2e-12 Wall-associated receptor kinase C-terminal
Pkinase PF00069 393 - 661 6.6e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 394 - 662 9.5e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1639, 2117
Acc36I ACCTGC 1 cut(s) 143
AccB1I GGYRCC 3 cut(s) 572, 1208, 1785
AccB7I CCANNNNNTGG 1 cut(s) 1868
AciI CCGC 3 cut(s) 138, 612, 620
AclWI GGATC 3 cut(s) 551, 1670, 1853
AcoI YGGCCR 2 cut(s) 1237, 1806
AcsI RAATTY 7 cut(s) 4, 270, 764, 1168, 1801, 1827, 2086
AcuI CTGAAG 1 cut(s) 1120
AcvI CACGTG 1 cut(s) 491
AcyI GRCGYC 2 cut(s) 573, 1661
AfaI GTAC 4 cut(s) 295, 434, 811, 1977
AfiI CCNNNNNNNGG 3 cut(s) 172, 173, 1868
AflIII ACRYGT 4 cut(s) 420, 490, 806, 1319
AgsI TTSAA 7 cut(s) 77, 674, 769, 1174, 1279, 1310, 1832
AjiI CACGTC 1 cut(s) 1322
AjnI CCWGG 2 cut(s) 460, 2049
Alw21I GWGCWC 1 cut(s) 2020
Alw26I GTCTC 4 cut(s) 551, 644, 1487, 1951
Alw44I GTGCAC 1 cut(s) 2016
AlwI GGATC 3 cut(s) 551, 1670, 1853
AlwNI CAGNNNCTG 1 cut(s) 1421
Ama87I CYCGRG 1 cut(s) 1679
AoxI GGCC 5 cut(s) 1237, 1466, 1806, 1923, 2074
ApaLI GTGCAC 1 cut(s) 2016
ApeKI GCWGC 5 cut(s) 120, 536, 968, 1939, 2139
ApoI RAATTY 7 cut(s) 4, 270, 764, 1168, 1801, 1827, 2086
ArsI GACNNNNNNTTYG 4 cut(s) 219, 251, 1076, 1108
AspLEI GCGC 3 cut(s) 92, 575, 1260
AspS9I GGNCC 1 cut(s) 2047
AsuC2I CCSGG 1 cut(s) 1665
AsuHPI GGTGA 3 cut(s) 238, 1855, 2097
AvaI CYCGRG 1 cut(s) 1679
AvaII GGWCC 1 cut(s) 2047
BaeGI GKGCMC 1 cut(s) 2020
BalI TGGCCA 1 cut(s) 1808
BanI GGYRCC 3 cut(s) 572, 1208, 1785
BbrPI CACGTG 1 cut(s) 491
BbsI GAAGAC 2 cut(s) 1126, 2013
Bbv12I GWGCWC 1 cut(s) 2020
BbvI GCAGC 5 cut(s) 132, 548, 980, 1951, 2151
BccI CCATC 5 cut(s) 656, 853, 1190, 1229, 1434
BceAI ACGGC 2 cut(s) 549, 1224
BciT130I CCWGG 2 cut(s) 462, 2051
BclI TGATCA 3 cut(s) 48, 243, 1627
BcnI CCSGG 1 cut(s) 1665
BcoDI GTCTC 4 cut(s) 551, 644, 1487, 1951
BfaI CTAG 3 cut(s) 327, 540, 2154
BfmI CTRYAG 2 cut(s) 118, 888
BfoI RGCGCY 2 cut(s) 576, 1261
BfuAI ACCTGC 1 cut(s) 143
BglI GCCNNNNNGGC 1 cut(s) 1814
BisI GCNGC 6 cut(s) 121, 537, 621, 969, 1940, 2140
BlsI GCNGC 6 cut(s) 122, 538, 622, 970, 1941, 2141
BmcAI AGTACT 2 cut(s) 295, 1977
Bme1390I CCNGG 3 cut(s) 462, 1665, 2051
Bme18I GGWCC 1 cut(s) 2047
BmeT110I CYCGRG 1 cut(s) 1679
BmgBI CACGTC 1 cut(s) 1322
BmgT120I GGNCC 1 cut(s) 2047
BmiI GGNNCC 4 cut(s) 574, 597, 1210, 1787
BmrFI CCNGG 3 cut(s) 462, 1665, 2051
BmrI ACTGGG 1 cut(s) 349
BmsI GCATC 4 cut(s) 428, 1065, 1346, 1492
BmuI ACTGGG 1 cut(s) 349
BpiI GAAGAC 2 cut(s) 1126, 2013
BplI GAGNNNNNCTC 2 cut(s) 1368, 1400
BpmI CTGGAG 2 cut(s) 647, 2000
BpuEI CTTGAG 2 cut(s) 487, 1348
BpuMI CCSGG 1 cut(s) 1665
BsaAI YACGTR 1 cut(s) 491
BsaHI GRCGYC 2 cut(s) 573, 1661
BsaI GGTCTC 1 cut(s) 1951
BsaJI CCNNGG 5 cut(s) 460, 1099, 1432, 1965, 2011
BsaWI WCCGGW 1 cut(s) 1929
BsaXI ACNNNNNCTCC 6 cut(s) 292, 322, 1009, 1039, 1116, 1146
Bsc4I CCNNNNNNNGG 3 cut(s) 172, 173, 1868
Bse118I RCCGGY 1 cut(s) 1788
Bse1I ACTGG 4 cut(s) 344, 560, 1512, 1983
Bse3DI GCAATG 3 cut(s) 448, 1482, 1531
BseBI CCWGG 2 cut(s) 462, 2051
BseDI CCNNGG 5 cut(s) 460, 1099, 1432, 1965, 2011
BseGI GGATG 8 cut(s) 443, 685, 845, 942, 1056, 1361, 1576, 1757
BseLI CCNNNNNNNGG 3 cut(s) 172, 173, 1868
BseMI GCAATG 3 cut(s) 448, 1482, 1531
BseMII CTCAG 3 cut(s) 992, 1469, 1474
BseNI ACTGG 4 cut(s) 344, 560, 1512, 1983
BseRI GAGGAG 1 cut(s) 1373
BseSI GKGCMC 1 cut(s) 2020
BseXI GCAGC 5 cut(s) 132, 548, 980, 1951, 2151
BsgI GTGCAG 2 cut(s) 660, 987
BshFI GGCC 5 cut(s) 1239, 1468, 1808, 1925, 2076
BshNI GGYRCC 3 cut(s) 572, 1208, 1785
BsiHKAI GWGCWC 1 cut(s) 2020
BsiHKCI CYCGRG 1 cut(s) 1679
BsiSI CCGG 4 cut(s) 781, 1664, 1789, 1930
BslFI GGGAC 4 cut(s) 221, 339, 443, 1672
BslI CCNNNNNNNGG 3 cut(s) 172, 173, 1868
BsmAI GTCTC 4 cut(s) 551, 644, 1487, 1951
BsmFI GGGAC 4 cut(s) 221, 339, 443, 1672
BsnI GGCC 5 cut(s) 1239, 1468, 1808, 1925, 2076
Bso31I GGTCTC 1 cut(s) 1951
BsoBI CYCGRG 1 cut(s) 1679
Bsp1286I GDGCHC 1 cut(s) 2020
Bsp143I GATC 8 cut(s) 48, 66, 243, 556, 1627, 1675, 1743, 1858
Bsp19I CCATGG 2 cut(s) 1432, 1965
BspACI CCGC 3 cut(s) 138, 612, 620
BspANI GGCC 5 cut(s) 1239, 1468, 1808, 1925, 2076
BspCNI CTCAG 3 cut(s) 991, 1470, 1473
BspLI GGNNCC 4 cut(s) 574, 597, 1210, 1787
BspMAI CTGCAG 1 cut(s) 122
BspMI ACCTGC 1 cut(s) 143
BspPI GGATC 3 cut(s) 551, 1670, 1853
BspQI GCTCTTC 1 cut(s) 826
BspT107I GGYRCC 3 cut(s) 572, 1208, 1785
BspTNI GGTCTC 1 cut(s) 1951
BsrDI GCAATG 3 cut(s) 448, 1482, 1531
BsrFI RCCGGY 1 cut(s) 1788
BsrI ACTGG 4 cut(s) 344, 560, 1512, 1983
BssAI RCCGGY 1 cut(s) 1788
BssECI CCNNGG 5 cut(s) 460, 1099, 1432, 1965, 2011
BssMI GATC 8 cut(s) 48, 66, 243, 556, 1627, 1675, 1743, 1858
BssNI GRCGYC 2 cut(s) 573, 1661
BssT1I CCWWGG 4 cut(s) 1099, 1432, 1965, 2011
Bst2UI CCWGG 2 cut(s) 462, 2051
Bst6I CTCTTC 3 cut(s) 398, 409, 826
BstACI GRCGYC 2 cut(s) 573, 1661
BstAPI GCANNNNNTGC 1 cut(s) 714
BstBAI YACGTR 1 cut(s) 491
BstC8I GCNNGC 1 cut(s) 1111
BstDEI CTNAG 3 cut(s) 978, 1460, 1478
BstDSI CCRYGG 2 cut(s) 1432, 1965
BstF5I GGATG 8 cut(s) 443, 685, 845, 942, 1056, 1361, 1576, 1757
BstH2I RGCGCY 2 cut(s) 576, 1261
BstHHI GCGC 3 cut(s) 92, 575, 1260
BstKTI GATC 8 cut(s) 51, 69, 246, 559, 1630, 1678, 1746, 1861
BstMAI GTCTC 4 cut(s) 551, 644, 1487, 1951
BstMBI GATC 8 cut(s) 48, 66, 243, 556, 1627, 1675, 1743, 1858
BstMWI GCNNNNNNNGC 8 cut(s) 150, 185, 504, 620, 714, 974, 1814, 1922
BstNI CCWGG 2 cut(s) 462, 2051
BstNSI RCATGY 2 cut(s) 225, 810
BstSCI CCNGG 3 cut(s) 460, 1663, 2049
BstSFI CTRYAG 2 cut(s) 118, 888
BstSLI GKGCMC 1 cut(s) 2020
BstV1I GCAGC 5 cut(s) 132, 548, 980, 1951, 2151
BstV2I GAAGAC 2 cut(s) 1126, 2013
BstXI CCANNNNNNTGG 1 cut(s) 1433
BsuRI GGCC 5 cut(s) 1239, 1468, 1808, 1925, 2076
BtgI CCRYGG 2 cut(s) 1432, 1965
BtgZI GCGATG 1 cut(s) 1505
BtrI CACGTC 1 cut(s) 1322
BtsCI GGATG 8 cut(s) 443, 685, 845, 942, 1056, 1361, 1576, 1757
BtsI GCAGTG 3 cut(s) 510, 1257, 2049
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 1 cut(s) 1111
CaiI CAGNNNCTG 1 cut(s) 1421
CfoI GCGC 3 cut(s) 92, 575, 1260
Cfr10I RCCGGY 1 cut(s) 1788
Cfr13I GGNCC 1 cut(s) 2047
CseI GACGC 3 cut(s) 133, 496, 1669
Csp6I GTAC 4 cut(s) 294, 433, 810, 1976
CviQI GTAC 4 cut(s) 294, 433, 810, 1976
DdeI CTNAG 3 cut(s) 978, 1460, 1478
DinI GGCGCC 1 cut(s) 574
DpnI GATC 8 cut(s) 50, 68, 245, 558, 1629, 1677, 1745, 1860
DpnII GATC 8 cut(s) 48, 66, 243, 556, 1627, 1675, 1743, 1858
DraI TTTAAA 1 cut(s) 1989
DrdI GACNNNNNNGTC 2 cut(s) 1639, 2117
DseDI GACNNNNNNGTC 2 cut(s) 1639, 2117
EaeI YGGCCR 2 cut(s) 1237, 1806
Eam1104I CTCTTC 3 cut(s) 398, 409, 826
EarI CTCTTC 3 cut(s) 398, 409, 826
Eco130I CCWWGG 4 cut(s) 1099, 1432, 1965, 2011
Eco31I GGTCTC 1 cut(s) 1951
Eco32I GATATC 1 cut(s) 349
Eco47I GGWCC 1 cut(s) 2047
Eco57I CTGAAG 1 cut(s) 1120
Eco72I CACGTG 1 cut(s) 491
Eco88I CYCGRG 1 cut(s) 1679
EcoRI GAATTC 3 cut(s) 4, 270, 764
EcoRII CCWGG 2 cut(s) 460, 2049
EcoRV GATATC 1 cut(s) 349
EcoT14I CCWWGG 4 cut(s) 1099, 1432, 1965, 2011
EgeI GGCGCC 1 cut(s) 574
EheI GGCGCC 1 cut(s) 574
ErhI CCWWGG 4 cut(s) 1099, 1432, 1965, 2011
FalI AAGNNNNNCTT 4 cut(s) 1045, 1077, 2061, 2093
FaqI GGGAC 4 cut(s) 221, 339, 443, 1672
FauI CCCGC 1 cut(s) 131
FauNDI CATATG 1 cut(s) 364
FbaI TGATCA 3 cut(s) 48, 243, 1627
Fnu4HI GCNGC 6 cut(s) 121, 537, 621, 969, 1940, 2140
FokI GGATG 8 cut(s) 450, 692, 832, 929, 1043, 1368, 1583, 1744
Fsp4HI GCNGC 6 cut(s) 121, 537, 621, 969, 1940, 2140
FspBI CTAG 3 cut(s) 327, 540, 2154
GlaI GCGC 3 cut(s) 91, 574, 1259
GluI GCNGC 6 cut(s) 121, 537, 621, 969, 1940, 2140
GsuI CTGGAG 2 cut(s) 647, 2000
HaeII RGCGCY 2 cut(s) 576, 1261
HaeIII GGCC 5 cut(s) 1239, 1468, 1808, 1925, 2076
HapII CCGG 4 cut(s) 781, 1664, 1789, 1930
HgaI GACGC 3 cut(s) 133, 496, 1669
HhaI GCGC 3 cut(s) 92, 575, 1260
Hin1I GRCGYC 2 cut(s) 573, 1661
Hin6I GCGC 3 cut(s) 90, 573, 1258
HinP1I GCGC 3 cut(s) 90, 573, 1258
HincII GTYRAC 2 cut(s) 1644, 1774
HindII GTYRAC 2 cut(s) 1644, 1774
HinfI GANTC 7 cut(s) 34, 608, 924, 991, 1336, 1448, 2111
HpaII CCGG 4 cut(s) 781, 1664, 1789, 1930
HphI GGTGA 3 cut(s) 238, 1855, 2097
Hpy166II GTNNAC 5 cut(s) 866, 1216, 1644, 1774, 2018
Hpy188I TCNGA 7 cut(s) 269, 417, 556, 607, 981, 1096, 1632
Hpy188III TCNNGA 8 cut(s) 275, 626, 855, 1325, 1365, 1568, 1679, 1741
Hpy8I GTNNAC 5 cut(s) 866, 1216, 1644, 1774, 2018
HpyAV CCTTC 4 cut(s) 1071, 1080, 1526, 1936
HpyCH4IV ACGT 6 cut(s) 234, 420, 490, 964, 1321, 1369
HpyF10VI GCNNNNNNNGC 8 cut(s) 150, 185, 504, 620, 714, 974, 1814, 1922
HpyF3I CTNAG 3 cut(s) 978, 1460, 1478
HpySE526I ACGT 6 cut(s) 234, 420, 490, 964, 1321, 1369
Hsp92I GRCGYC 2 cut(s) 573, 1661
HspAI GCGC 3 cut(s) 90, 573, 1258
KasI GGCGCC 1 cut(s) 572
Ksp22I TGATCA 3 cut(s) 48, 243, 1627
Kzo9I GATC 8 cut(s) 48, 66, 243, 556, 1627, 1675, 1743, 1858
LguI GCTCTTC 1 cut(s) 826
LmnI GCTCC 6 cut(s) 141, 176, 847, 1288, 1654, 2060
Lsp1109I GCAGC 5 cut(s) 132, 548, 980, 1951, 2151
LweI GCATC 4 cut(s) 428, 1065, 1346, 1492
MaeI CTAG 3 cut(s) 327, 540, 2154
MaeII ACGT 6 cut(s) 234, 420, 490, 964, 1321, 1369
MaeIII GTNAC 7 cut(s) 217, 451, 475, 508, 1021, 1608, 2119
MalI GATC 8 cut(s) 50, 68, 245, 558, 1629, 1677, 1745, 1860
MboI GATC 8 cut(s) 48, 66, 243, 556, 1627, 1675, 1743, 1858
MhlI GDGCHC 1 cut(s) 2020
MlsI TGGCCA 1 cut(s) 1808
MluNI TGGCCA 1 cut(s) 1808
Mly113I GGCGCC 1 cut(s) 573
MlyI GAGTC 2 cut(s) 1457, 2105
Mox20I TGGCCA 1 cut(s) 1808
MscI TGGCCA 1 cut(s) 1808
MseI TTAA 4 cut(s) 80, 1077, 1316, 1988
MslI CAYNNNNRTG 5 cut(s) 917, 1431, 1524, 1542, 1791
Msp20I TGGCCA 1 cut(s) 1808
MspA1I CMGCKG 2 cut(s) 1499, 1910
MspI CCGG 4 cut(s) 781, 1664, 1789, 1930
MspR9I CCNGG 3 cut(s) 462, 1665, 2051
MvaI CCWGG 2 cut(s) 462, 2051
MwoI GCNNNNNNNGC 8 cut(s) 150, 185, 504, 620, 714, 974, 1814, 1922
NarI GGCGCC 1 cut(s) 573
NciI CCSGG 1 cut(s) 1665
NcoI CCATGG 2 cut(s) 1432, 1965
NdeI CATATG 1 cut(s) 364
NdeII GATC 8 cut(s) 48, 66, 243, 556, 1627, 1675, 1743, 1858
NlaIV GGNNCC 4 cut(s) 574, 597, 1210, 1787
NmeAIII GCCGAG 1 cut(s) 1400
NmuCI GTSAC 5 cut(s) 217, 475, 508, 1608, 2119
NspI RCATGY 2 cut(s) 225, 810
PciI ACATGT 1 cut(s) 806
PciSI GCTCTTC 1 cut(s) 826
PcsI WCGNNNNNNNCGW 1 cut(s) 1743
PfeI GAWTC 5 cut(s) 34, 608, 924, 991, 1336
PflMI CCANNNNNTGG 1 cut(s) 1868
PfoI TCCNGGA 1 cut(s) 2049
PkrI GCNGC 6 cut(s) 122, 538, 622, 970, 1941, 2141
PleI GAGTC 2 cut(s) 1456, 2105
PluTI GGCGCC 1 cut(s) 576
PmaCI CACGTG 1 cut(s) 491
PmlI CACGTG 1 cut(s) 491
PpsI GAGTC 2 cut(s) 1456, 2105
Ppu21I YACGTR 1 cut(s) 491
PscI ACATGT 1 cut(s) 806
Psp6I CCWGG 2 cut(s) 460, 2049
PspCI CACGTG 1 cut(s) 491
PspGI CCWGG 2 cut(s) 460, 2049
PspN4I GGNNCC 4 cut(s) 574, 597, 1210, 1787
PspPI GGNCC 1 cut(s) 2047
PstI CTGCAG 1 cut(s) 122
PstNI CAGNNNCTG 1 cut(s) 1421
PvuII CAGCTG 2 cut(s) 1499, 1910
RsaI GTAC 4 cut(s) 295, 434, 811, 1977
RsaNI GTAC 4 cut(s) 294, 433, 810, 1976
RseI CAYNNNNRTG 5 cut(s) 917, 1431, 1524, 1542, 1791
SapI GCTCTTC 1 cut(s) 826
SaqAI TTAA 4 cut(s) 80, 1077, 1316, 1988
SatI GCNGC 6 cut(s) 121, 537, 621, 969, 1940, 2140
Sau3AI GATC 8 cut(s) 48, 66, 243, 556, 1627, 1675, 1743, 1858
Sau96I GGNCC 1 cut(s) 2047
ScaI AGTACT 2 cut(s) 295, 1977
SchI GAGTC 2 cut(s) 1457, 2105
ScrFI CCNGG 3 cut(s) 462, 1665, 2051
SduI GDGCHC 1 cut(s) 2020
SfaNI GCATC 4 cut(s) 428, 1065, 1346, 1492
SfcI CTRYAG 2 cut(s) 118, 888
SfoI GGCGCC 1 cut(s) 574
SinI GGWCC 1 cut(s) 2047
SmiMI CAYNNNNRTG 5 cut(s) 917, 1431, 1524, 1542, 1791
SmlI CTYRAG 2 cut(s) 502, 1363
SmoI CTYRAG 2 cut(s) 502, 1363
SsiI CCGC 3 cut(s) 138, 612, 620
SspDI GGCGCC 1 cut(s) 572
SspMI CTAG 3 cut(s) 327, 540, 2154
StyD4I CCNGG 3 cut(s) 460, 1663, 2049
StyI CCWWGG 4 cut(s) 1099, 1432, 1965, 2011
TaiI ACGT 6 cut(s) 237, 423, 493, 967, 1324, 1372
TaqI TCGA 6 cut(s) 65, 359, 381, 797, 1031, 1746
TatI WGTACW 3 cut(s) 293, 809, 1975
TauI GCSGC 1 cut(s) 623
TfiI GAWTC 5 cut(s) 34, 608, 924, 991, 1336
Tru1I TTAA 4 cut(s) 80, 1077, 1316, 1988
Tru9I TTAA 4 cut(s) 80, 1077, 1316, 1988
TseFI GTSAC 5 cut(s) 217, 475, 508, 1608, 2119
TseI GCWGC 5 cut(s) 120, 536, 968, 1939, 2139
Tsp45I GTSAC 5 cut(s) 217, 475, 508, 1608, 2119
TspDTI ATGAA 8 cut(s) 17, 780, 1281, 1313, 1410, 1508, 2019, 2082
TspGWI ACGGA 2 cut(s) 12, 450
Van91I CCANNNNNTGG 1 cut(s) 1868
VneI GTGCAC 1 cut(s) 2016
VpaK11BI GGWCC 1 cut(s) 2047
XapI RAATTY 7 cut(s) 4, 270, 764, 1168, 1801, 1827, 2086
XceI RCATGY 2 cut(s) 225, 810
XspI CTAG 3 cut(s) 327, 540, 2154
ZrmI AGTACT 2 cut(s) 295, 1977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.