Prupe.8G218900_v2.0.a1

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0003318
Physical Location & Seq
Forward (+)
19951580 .. 19954143
2564 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G218900.1

Sequence Viewer

Length: 1392 bp
ATGCATCAAACAATGGCTCTGCAAAAACATTTGGTCTTGTTCTATGTTGTTGTCTCTTTCTGTGCTGCTTCATGTTACAGTAGTGGTTTCCAAGAGGTCAACTCACTTCATAGTTTTGTTGACCATGAAAAAGAGTCTGGCTATAATTCTAGAGCTCATCCTTCAAACATGAACACCATTGAAGGTGTCAAGTTCATGGAATTCATCAAGCCAAGAGCTCAGCTCTTCAGTTCGAGGAAGCTCGAAAGAGCGGCCGGCAGCAAGTCATCAAGCTCGGTCAAAACGATTAGTGTTGCTAATTTTGGAGCTAAAGGAAATGGTGCCGATGACACACGGGCATTTGAGAAGGCGTGGAAGGCAGCTTGTTCTTCCAATGGAGCCATAGTTCTTGTGGTGCCACAAAAGACCTATCTTGTTAGGCCGATCGAATTCTCGGGCCCCTGCAAATCTCATCTAACAATGCAGATATATGGAACCATAGAAGCATCAGATGATCGGTCAGTCTACAAAGATGTCACGCATTGGCTTATCTTTGACAACGTCCAAAGCTTGTTAGTTGTAGGTCCTGGAACCATCAATGGCAATGGAAATAGATGGTGGGAAAACTCATGCAAAAGAAAGCCTCAGCCCCCCTGCAACGAACAGGCCCCCACGGCTGTGACCTTCAACAAGTGCAATAACTTGGTGGTGAAGAATCTGAAGATCCAAGACGCACAGCAAATGCATGTTAGATTTCAAAATTGTAAAAATGTTGAAGCTTCCCATCTTACAGTAACTGCACCAGAAGATAGTCCTAATACTGATGGAATTCATATCACAAATACCAAGAACATTACCATCTCAAGCTCTGTTATAGGAACAGGTGATGATTGCATCTCTATTGTAAGTGGGTCCCAAAGAGTGCAAGCTACAGACATAACTTGTGGACCAGGCCATGGAATCAGTATTGGTAGCTTGGGAGAAGACAATGCAAATGACCATGTTTCAGGAGTATTTGTGAATGGGGCTAAGATTTCTGGTACCTCAAATGGAGTTAGGATCAAGACATGGCAGGGAGGTTCAGGCAGTGCAAGCAATATTGTTTTCCAGAATGTGGAAATGAATGATGTGACTAACCCTATCATCATAGACCAAAATTATTGTGACCACAAAAACAAGGATTGTACACGACAGAGGTCGGCAGTTCAAGTGAAAAATGTGTTGTACCAGAACATAAGAGGGACAAGTGCTTCAACTGATGCCATAACATTTAATTGCAGCCAAAGTGTTCCATGTCAAGGCATTGTGCTTCAAAATATTCAACTGCAAAATGCAAGAGCCAAATGCAACAATGTTAAACCTGCGTACAAAGGCGCTGTCTCTCCTCGATGTTCTTGGGGATTAGTAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

464

Amino Acids

50.28

Weight (kDa)

8.85

Isoelectric Point (pI)

36.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1348
Acc65I GGTACC 1 cut(s) 1019
AccB1I GGYRCC 3 cut(s) 320, 394, 1019
AccB7I CCANNNNNTGG 2 cut(s) 935, 1093
AccBSI CCGCTC 1 cut(s) 251
AccI GTMKAC 1 cut(s) 504
AciI CCGC 1 cut(s) 251
AclWI GGATC 2 cut(s) 697, 1046
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 3 cut(s) 200, 428, 807
AcuI CTGAAG 2 cut(s) 211, 719
AfaI GTAC 4 cut(s) 1021, 1165, 1205, 1346
AfiI CCNNNNNNNGG 3 cut(s) 935, 1093, 1277
AgsI TTSAA 9 cut(s) 165, 182, 667, 737, 755, 1187, 1233, 1292, 1301
AjnI CCWGG 2 cut(s) 565, 928
AleI CACNNNNGTG 1 cut(s) 656
AloI GAACNNNNNNTCC 2 cut(s) 369, 401
Alw21I GWGCWC 2 cut(s) 157, 220
Alw26I GTCTC 2 cut(s) 58, 1363
AlwI GGATC 2 cut(s) 697, 1046
AlwNI CAGNNNCTG 1 cut(s) 776
Ama87I CYCGRG 1 cut(s) 433
AoxI GGCC 5 cut(s) 252, 419, 436, 645, 931
ApaI GGGCCC 1 cut(s) 440
ApeKI GCWGC 4 cut(s) 65, 258, 359, 1257
ApoI RAATTY 3 cut(s) 200, 428, 807
ArsI GACNNNNNNTTYG 2 cut(s) 1341, 1373
Asp718I GGTACC 1 cut(s) 1019
AspLEI GCGC 1 cut(s) 1355
AspS9I GGNCC 6 cut(s) 436, 437, 563, 646, 891, 926
AsuHPI GGTGA 2 cut(s) 700, 875
AvaI CYCGRG 1 cut(s) 433
AvaII GGWCC 3 cut(s) 563, 891, 926
BaeGI GKGCMC 1 cut(s) 440
BanI GGYRCC 3 cut(s) 320, 394, 1019
BanII GRGCYC 3 cut(s) 157, 220, 440
BbsI GAAGAC 1 cut(s) 969
Bbv12I GWGCWC 2 cut(s) 157, 220
BbvCI CCTCAGC 1 cut(s) 624
BbvI GCAGC 4 cut(s) 52, 270, 371, 1269
BccI CCATC 5 cut(s) 581, 588, 771, 797, 845
BceAI ACGGC 1 cut(s) 669
BciT130I CCWGG 2 cut(s) 567, 930
BcoDI GTCTC 2 cut(s) 58, 1363
BfaI CTAG 1 cut(s) 150
BfmI CTRYAG 1 cut(s) 909
BfoI RGCGCY 1 cut(s) 1356
BfuAI ACCTGC 1 cut(s) 1348
BglI GCCNNNNNGGC 1 cut(s) 653
BisI GCNGC 5 cut(s) 66, 252, 259, 360, 1258
BlpI GCTNAGC 1 cut(s) 219
BlsI GCNGC 5 cut(s) 67, 253, 260, 361, 1259
Bme1390I CCNGG 2 cut(s) 567, 930
Bme18I GGWCC 3 cut(s) 563, 891, 926
BmeT110I CYCGRG 1 cut(s) 433
BmgT120I GGNCC 6 cut(s) 436, 437, 563, 646, 891, 926
BmrFI CCNGG 2 cut(s) 567, 930
BmsI GCATC 4 cut(s) 13, 494, 882, 1228
BoxI GACNNNNGTC 1 cut(s) 1174
BpiI GAAGAC 1 cut(s) 969
BplI GAGNNNNNCTC 4 cut(s) 86, 118, 207, 239
Bpu10I CCTNAGC 1 cut(s) 624
Bpu1102I GCTNAGC 1 cut(s) 219
BpuEI CTTGAG 1 cut(s) 826
BsaJI CCNNGG 2 cut(s) 651, 934
BsaXI ACNNNNNCTCC 4 cut(s) 369, 399, 981, 1011
Bsc4I CCNNNNNNNGG 3 cut(s) 935, 1093, 1277
Bse118I RCCGGY 1 cut(s) 254
Bse3DI GCAATG 1 cut(s) 589
BseBI CCWGG 2 cut(s) 567, 930
BseDI CCNNGG 2 cut(s) 651, 934
BseGI GGATG 1 cut(s) 157
BseLI CCNNNNNNNGG 3 cut(s) 935, 1093, 1277
BseMI GCAATG 1 cut(s) 589
BseMII CTCAG 2 cut(s) 233, 638
BseRI GAGGAG 1 cut(s) 1353
BseSI GKGCMC 1 cut(s) 440
BseX3I CGGCCG 1 cut(s) 252
BseXI GCAGC 4 cut(s) 52, 270, 371, 1269
BsgI GTGCAG 1 cut(s) 762
Bsh1285I CGRYCG 2 cut(s) 255, 426
BshFI GGCC 5 cut(s) 254, 421, 438, 647, 933
BshNI GGYRCC 3 cut(s) 320, 394, 1019
BsiEI CGRYCG 2 cut(s) 255, 426
BsiHKAI GWGCWC 2 cut(s) 157, 220
BsiHKCI CYCGRG 1 cut(s) 433
BsiSI CCGG 1 cut(s) 255
BslFI GGGAC 2 cut(s) 877, 1234
BslI CCNNNNNNNGG 3 cut(s) 935, 1093, 1277
BsmAI GTCTC 2 cut(s) 58, 1363
BsmFI GGGAC 2 cut(s) 877, 1234
BsnI GGCC 5 cut(s) 254, 421, 438, 647, 933
BsoBI CYCGRG 1 cut(s) 433
Bsp120I GGGCCC 1 cut(s) 436
Bsp1286I GDGCHC 3 cut(s) 157, 220, 440
Bsp1407I TGTACA 1 cut(s) 1163
Bsp143I GATC 4 cut(s) 423, 493, 702, 1038
Bsp1720I GCTNAGC 1 cut(s) 219
Bsp19I CCATGG 1 cut(s) 934
BspACI CCGC 1 cut(s) 251
BspANI GGCC 5 cut(s) 254, 421, 438, 647, 933
BspCNI CTCAG 2 cut(s) 232, 637
BspMI ACCTGC 1 cut(s) 1348
BspPI GGATC 2 cut(s) 697, 1046
BspQI GCTCTTC 1 cut(s) 230
BspT107I GGYRCC 3 cut(s) 320, 394, 1019
BsrBI CCGCTC 1 cut(s) 251
BsrDI GCAATG 1 cut(s) 589
BsrFI RCCGGY 1 cut(s) 254
BsrGI TGTACA 1 cut(s) 1163
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 2 cut(s) 651, 934
BssMI GATC 4 cut(s) 423, 493, 702, 1038
BssT1I CCWWGG 1 cut(s) 934
Bst2UI CCWGG 2 cut(s) 567, 930
Bst4CI ACNGT 2 cut(s) 80, 772
Bst6I CTCTTC 1 cut(s) 230
BstAUI TGTACA 1 cut(s) 1163
BstC8I GCNNGC 3 cut(s) 256, 906, 1072
BstDEI CTNAG 3 cut(s) 219, 624, 1008
BstDSI CCRYGG 2 cut(s) 651, 934
BstF5I GGATG 1 cut(s) 157
BstH2I RGCGCY 1 cut(s) 1356
BstHHI GCGC 1 cut(s) 1355
BstKTI GATC 4 cut(s) 426, 496, 705, 1041
BstMAI GTCTC 2 cut(s) 58, 1363
BstMBI GATC 4 cut(s) 423, 493, 702, 1038
BstMCI CGRYCG 2 cut(s) 255, 426
BstMWI GCNNNNNNNGC 3 cut(s) 356, 653, 1071
BstNI CCWGG 2 cut(s) 567, 930
BstNSI RCATGY 1 cut(s) 728
BstPAI GACNNNNGTC 1 cut(s) 1174
BstSCI CCNGG 2 cut(s) 565, 928
BstSFI CTRYAG 1 cut(s) 909
BstSLI GKGCMC 1 cut(s) 440
BstV1I GCAGC 4 cut(s) 52, 270, 371, 1269
BstV2I GAAGAC 1 cut(s) 969
BstX2I RGATCY 1 cut(s) 702
BstYI RGATCY 1 cut(s) 702
BstZI CGGCCG 1 cut(s) 252
BsuRI GGCC 5 cut(s) 254, 421, 438, 647, 933
BtgI CCRYGG 2 cut(s) 651, 934
BtsCI GGATG 1 cut(s) 157
BtsI GCAGTG 1 cut(s) 1072
BtsIMutI CAGTG 1 cut(s) 1072
BveI ACCTGC 1 cut(s) 1348
Cac8I GCNNGC 3 cut(s) 256, 906, 1072
CaiI CAGNNNCTG 1 cut(s) 776
CfoI GCGC 1 cut(s) 1355
Cfr10I RCCGGY 1 cut(s) 254
Cfr13I GGNCC 6 cut(s) 436, 437, 563, 646, 891, 926
CseI GACGC 1 cut(s) 719
Csp6I GTAC 4 cut(s) 1020, 1164, 1204, 1345
CviQI GTAC 4 cut(s) 1020, 1164, 1204, 1345
DdeI CTNAG 3 cut(s) 219, 624, 1008
DpnI GATC 4 cut(s) 425, 495, 704, 1040
DpnII GATC 4 cut(s) 423, 493, 702, 1038
EaeI YGGCCR 1 cut(s) 252
EagI CGGCCG 1 cut(s) 252
Eam1104I CTCTTC 1 cut(s) 230
EarI CTCTTC 1 cut(s) 230
Ecl136II GAGCTC 2 cut(s) 155, 218
EclXI CGGCCG 1 cut(s) 252
Eco130I CCWWGG 1 cut(s) 934
Eco24I GRGCYC 3 cut(s) 157, 220, 440
Eco47I GGWCC 3 cut(s) 563, 891, 926
Eco52I CGGCCG 1 cut(s) 252
Eco53kI GAGCTC 2 cut(s) 155, 218
Eco57I CTGAAG 2 cut(s) 211, 719
Eco88I CYCGRG 1 cut(s) 433
EcoICRI GAGCTC 2 cut(s) 155, 218
EcoO109I RGGNCCY 4 cut(s) 437, 563, 646, 891
EcoRI GAATTC 3 cut(s) 200, 428, 807
EcoRII CCWGG 2 cut(s) 565, 928
EcoT14I CCWWGG 1 cut(s) 934
EcoT22I ATGCAT 2 cut(s) 6, 726
EcoT38I GRGCYC 3 cut(s) 157, 220, 440
ErhI CCWWGG 1 cut(s) 934
FaqI GGGAC 2 cut(s) 877, 1234
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 5 cut(s) 66, 252, 259, 360, 1258
FokI GGATG 1 cut(s) 144
FriOI GRGCYC 3 cut(s) 157, 220, 440
Fsp4HI GCNGC 5 cut(s) 66, 252, 259, 360, 1258
FspBI CTAG 1 cut(s) 150
GlaI GCGC 1 cut(s) 1354
GluI GCNGC 5 cut(s) 66, 252, 259, 360, 1258
HaeII RGCGCY 1 cut(s) 1356
HaeIII GGCC 5 cut(s) 254, 421, 438, 647, 933
HapII CCGG 1 cut(s) 255
HgaI GACGC 1 cut(s) 719
HhaI GCGC 1 cut(s) 1355
Hin6I GCGC 1 cut(s) 1353
HinP1I GCGC 1 cut(s) 1353
HincII GTYRAC 2 cut(s) 100, 121
HindII GTYRAC 2 cut(s) 100, 121
HindIII AAGCTT 2 cut(s) 547, 756
HinfI GANTC 3 cut(s) 134, 694, 939
HpaII CCGG 1 cut(s) 255
HphI GGTGA 2 cut(s) 700, 875
Hpy166II GTNNAC 5 cut(s) 100, 121, 505, 926, 1166
Hpy188I TCNGA 2 cut(s) 490, 699
Hpy188III TCNNGA 4 cut(s) 150, 987, 1042, 1087
Hpy8I GTNNAC 5 cut(s) 100, 121, 505, 926, 1166
HpyAV CCTTC 5 cut(s) 171, 176, 340, 349, 673
HpyCH4III ACNGT 2 cut(s) 80, 772
HpyCH4IV ACGT 1 cut(s) 540
HpyF10VI GCNNNNNNNGC 3 cut(s) 356, 653, 1071
HpyF3I CTNAG 3 cut(s) 219, 624, 1008
HpySE526I ACGT 1 cut(s) 540
HspAI GCGC 1 cut(s) 1353
KflI GGGWCCC 1 cut(s) 891
KpnI GGTACC 1 cut(s) 1023
KroI GCCGGC 1 cut(s) 254
KroNI GCCGGC 1 cut(s) 256
Kzo9I GATC 4 cut(s) 423, 493, 702, 1038
LguI GCTCTTC 1 cut(s) 230
LmnI GCTCC 2 cut(s) 305, 377
Lsp1109I GCAGC 4 cut(s) 52, 270, 371, 1269
LweI GCATC 4 cut(s) 13, 494, 882, 1228
MaeI CTAG 1 cut(s) 150
MaeII ACGT 1 cut(s) 540
MaeIII GTNAC 6 cut(s) 74, 514, 658, 772, 1108, 1142
MalI GATC 4 cut(s) 425, 495, 704, 1040
MbiI CCGCTC 1 cut(s) 251
MboI GATC 4 cut(s) 423, 493, 702, 1038
MboII GAAGA 6 cut(s) 217, 360, 703, 712, 797, 974
MflI RGATCY 1 cut(s) 702
MhlI GDGCHC 3 cut(s) 157, 220, 440
MluCI AATT 9 cut(s) 145, 200, 298, 428, 739, 807, 1135, 1252, 1387
MlyI GAGTC 1 cut(s) 143
MnlI CCTC 8 cut(s) 88, 228, 633, 1033, 1049, 1167, 1211, 1374
Mph1103I ATGCAT 2 cut(s) 6, 726
MroNI GCCGGC 1 cut(s) 254
MseI TTAA 3 cut(s) 1251, 1335, 1390
MslI CAYNNNNRTG 1 cut(s) 656
MspI CCGG 1 cut(s) 255
MspR9I CCNGG 2 cut(s) 567, 930
MvaI CCWGG 2 cut(s) 567, 930
MwoI GCNNNNNNNGC 3 cut(s) 356, 653, 1071
NaeI GCCGGC 1 cut(s) 256
NcoI CCATGG 1 cut(s) 934
NdeII GATC 4 cut(s) 423, 493, 702, 1038
NgoMIV GCCGGC 1 cut(s) 254
NmuCI GTSAC 4 cut(s) 514, 658, 1108, 1142
NsiI ATGCAT 2 cut(s) 6, 726
NspI RCATGY 1 cut(s) 728
OliI CACNNNNGTG 1 cut(s) 656
PciSI GCTCTTC 1 cut(s) 230
PcsI WCGNNNNNNNCGW 1 cut(s) 281
PdiI GCCGGC 1 cut(s) 256
PfeI GAWTC 2 cut(s) 694, 939
PflFI GACNNNGTC 1 cut(s) 539
PflMI CCANNNNNTGG 2 cut(s) 935, 1093
PfoI TCCNGGA 1 cut(s) 565
PkrI GCNGC 5 cut(s) 67, 253, 260, 361, 1259
Ple19I CGATCG 1 cut(s) 426
PleI GAGTC 1 cut(s) 142
PpsI GAGTC 1 cut(s) 142
PpuMI RGGWCCY 2 cut(s) 563, 891
PshAI GACNNNNGTC 1 cut(s) 1174
Psp124BI GAGCTC 2 cut(s) 157, 220
Psp5II RGGWCCY 2 cut(s) 563, 891
Psp6I CCWGG 2 cut(s) 565, 928
PspGI CCWGG 2 cut(s) 565, 928
PspOMI GGGCCC 1 cut(s) 436
PspPI GGNCC 6 cut(s) 436, 437, 563, 646, 891, 926
PspPPI RGGWCCY 2 cut(s) 563, 891
PstNI CAGNNNCTG 1 cut(s) 776
PsuI RGATCY 1 cut(s) 702
PsyI GACNNNGTC 1 cut(s) 539
PvuI CGATCG 1 cut(s) 426
RsaI GTAC 4 cut(s) 1021, 1165, 1205, 1346
RsaNI GTAC 4 cut(s) 1020, 1164, 1204, 1345
RseI CAYNNNNRTG 1 cut(s) 656
SacI GAGCTC 2 cut(s) 157, 220
SapI GCTCTTC 1 cut(s) 230
SaqAI TTAA 3 cut(s) 1251, 1335, 1390
SatI GCNGC 5 cut(s) 66, 252, 259, 360, 1258
Sau3AI GATC 4 cut(s) 423, 493, 702, 1038
Sau96I GGNCC 6 cut(s) 436, 437, 563, 646, 891, 926
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 2 cut(s) 567, 930
SduI GDGCHC 3 cut(s) 157, 220, 440
SfaNI GCATC 4 cut(s) 13, 494, 882, 1228
SfcI CTRYAG 1 cut(s) 909
SinI GGWCC 3 cut(s) 563, 891, 926
SmiMI CAYNNNNRTG 1 cut(s) 656
SmlI CTYRAG 1 cut(s) 841
SmoI CTYRAG 1 cut(s) 841
Sse9I AATT 9 cut(s) 145, 200, 298, 428, 739, 807, 1135, 1252, 1387
SsiI CCGC 1 cut(s) 251
SspI AATATT 2 cut(s) 1078, 1297
SspMI CTAG 1 cut(s) 150
SstI GAGCTC 2 cut(s) 157, 220
StyD4I CCNGG 2 cut(s) 565, 928
StyI CCWWGG 1 cut(s) 934
TaaI ACNGT 2 cut(s) 80, 772
TaiI ACGT 1 cut(s) 543
TaqI TCGA 4 cut(s) 233, 243, 426, 1366
TaqII GACCGA 2 cut(s) 265, 486
TasI AATT 9 cut(s) 145, 200, 298, 428, 739, 807, 1135, 1252, 1387
TatI WGTACW 1 cut(s) 1163
TauI GCSGC 1 cut(s) 254
TfiI GAWTC 2 cut(s) 694, 939
Tru1I TTAA 3 cut(s) 1251, 1335, 1390
Tru9I TTAA 3 cut(s) 1251, 1335, 1390
TscAI CASTG 1 cut(s) 1072
TseFI GTSAC 4 cut(s) 514, 658, 1108, 1142
TseI GCWGC 4 cut(s) 65, 258, 359, 1257
Tsp45I GTSAC 4 cut(s) 514, 658, 1108, 1142
TspDTI ATGAA 8 cut(s) 60, 98, 141, 184, 185, 193, 800, 1115
TspRI CASTG 1 cut(s) 1072
Tth111I GACNNNGTC 1 cut(s) 539
Van91I CCANNNNNTGG 2 cut(s) 935, 1093
VpaK11BI GGWCC 3 cut(s) 563, 891, 926
XapI RAATTY 3 cut(s) 200, 428, 807
XbaI TCTAGA 1 cut(s) 149
XceI RCATGY 1 cut(s) 728
XcmI CCANNNNNNNNNTGG 1 cut(s) 388
XmiI GTMKAC 1 cut(s) 504
XspI CTAG 1 cut(s) 150
Zsp2I ATGCAT 2 cut(s) 6, 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.