FvH4_2g25600

Universal stress protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003052
Physical Location & Seq
Forward (+)
20717058 .. 20719076
2019 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25600.t1

Sequence Viewer

Length: 480 bp
ATGGCAGAGAAGCAAGTGATGATTTTGGCGGCGGACGACAGCGAGCACAGCGCCTACGCGCTGCAGTGGACTGTGGAGCATTTCTTCGCGCCGTTCGGCTCCAACACGCCGTTCAAGCTCGTCATCGTCCACGCCAAGCCTACGCCGTCCTCCGTCGTCGGCCTCGCCGGTCCCGGGGCTGCTGAGGTTATGCCGATTGTGGATGCTGATTTGAAGAAGCAGGCTGCTCGCGTTAGTGAGAAGGCCAAGGAGTTTTGTGCTTCTAAATCGGTAAAAGATGTGGTCATAGAGGTGATAGAAGGCGATGCCAGGAATGTTCTCTGTGAGGCTGTTGAAAGACACCATGCATCCGTCTTGGTTGTGGGTAGTCATGGCTATGGAGCCATCAAAAGGGCGGTTTTGGGAAGTGTAAGTGACTATTGTGCTCATCACGCTCACTGCAGTGTTATGATCGTGAAGAGGCCTAAAACCAAACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

160

Amino Acids

17.07

Weight (kDa)

7.77

Isoelectric Point (pI)

28.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 8 - 153 3.9e-26 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 59, 89, 231
AciI CCGC 3 cut(s) 29, 32, 395
AfiI CCNNNNNNNGG 2 cut(s) 174, 390
AgsI TTSAA 3 cut(s) 115, 214, 335
AjnI CCWGG 1 cut(s) 308
AjuI GAANNNNNNNTTGG 2 cut(s) 95, 127
AleI CACNNNNGTG 1 cut(s) 441
AluBI AGCT 1 cut(s) 118
AluI AGCT 1 cut(s) 118
Alw21I GWGCWC 2 cut(s) 48, 427
Ama87I CYCGRG 1 cut(s) 173
AoxI GGCC 3 cut(s) 160, 243, 461
ApeKI GCWGC 3 cut(s) 61, 179, 224
AspLEI GCGC 3 cut(s) 53, 61, 91
AspS9I GGNCC 1 cut(s) 170
AsuC2I CCSGG 2 cut(s) 174, 175
AsuHPI GGTGA 1 cut(s) 304
AvaI CYCGRG 1 cut(s) 173
AvaII GGWCC 1 cut(s) 170
Bbv12I GWGCWC 2 cut(s) 48, 427
BbvCI CCTCAGC 1 cut(s) 183
BbvI GCAGC 3 cut(s) 48, 166, 211
BccI CCATC 1 cut(s) 392
BceAI ACGGC 3 cut(s) 76, 94, 130
BcgI CGANNNNNNTGC 2 cut(s) 209, 243
BciT130I CCWGG 1 cut(s) 310
BcnI CCSGG 2 cut(s) 174, 175
BfmI CTRYAG 2 cut(s) 62, 439
BfoI RGCGCY 1 cut(s) 54
BisI GCNGC 4 cut(s) 30, 62, 180, 225
BlsI GCNGC 4 cut(s) 31, 63, 181, 226
Bme1390I CCNGG 3 cut(s) 174, 175, 310
Bme18I GGWCC 1 cut(s) 170
BmeT110I CYCGRG 1 cut(s) 173
BmgT120I GGNCC 1 cut(s) 170
BmiI GGNNCC 3 cut(s) 100, 172, 382
BmrFI CCNGG 3 cut(s) 174, 175, 310
BmsI GCATC 3 cut(s) 193, 295, 356
Bpu10I CCTNAGC 1 cut(s) 183
BpuMI CCSGG 2 cut(s) 174, 175
BsaJI CCNNGG 3 cut(s) 173, 174, 246
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 390
Bse118I RCCGGY 1 cut(s) 167
BseBI CCWGG 1 cut(s) 310
BseDI CCNNGG 3 cut(s) 173, 174, 246
BseGI GGATG 2 cut(s) 208, 347
BseLI CCNNNNNNNGG 2 cut(s) 174, 390
BseMII CTCAG 1 cut(s) 174
BseXI GCAGC 3 cut(s) 48, 166, 211
Bsh1236I CGCG 3 cut(s) 59, 89, 231
BshFI GGCC 3 cut(s) 162, 245, 463
BsiHKAI GWGCWC 2 cut(s) 48, 427
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 2 cut(s) 168, 174
BslFI GGGAC 1 cut(s) 156
BslI CCNNNNNNNGG 2 cut(s) 174, 390
BsmFI GGGAC 1 cut(s) 156
BsnI GGCC 3 cut(s) 162, 245, 463
BsoBI CYCGRG 1 cut(s) 173
Bsp1286I GDGCHC 2 cut(s) 48, 427
Bsp143I GATC 1 cut(s) 450
BspACI CCGC 3 cut(s) 29, 32, 395
BspANI GGCC 3 cut(s) 162, 245, 463
BspCNI CTCAG 1 cut(s) 175
BspFNI CGCG 3 cut(s) 59, 89, 231
BspLI GGNNCC 3 cut(s) 100, 172, 382
BspMAI CTGCAG 2 cut(s) 66, 443
BsrFI RCCGGY 1 cut(s) 167
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 3 cut(s) 173, 174, 246
BssMI GATC 1 cut(s) 450
BssT1I CCWWGG 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 310
Bst4CI ACNGT 1 cut(s) 73
Bst6I CTCTTC 1 cut(s) 452
BstC8I GCNNGC 3 cut(s) 44, 222, 229
BstDEI CTNAG 1 cut(s) 183
BstF5I GGATG 2 cut(s) 208, 347
BstFNI CGCG 3 cut(s) 59, 89, 231
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 3 cut(s) 53, 61, 91
BstKTI GATC 1 cut(s) 453
BstMBI GATC 1 cut(s) 450
BstMWI GCNNNNNNNGC 3 cut(s) 48, 115, 431
BstNI CCWGG 1 cut(s) 310
BstSCI CCNGG 3 cut(s) 172, 173, 308
BstSFI CTRYAG 2 cut(s) 62, 439
BstUI CGCG 3 cut(s) 59, 89, 231
BstV1I GCAGC 3 cut(s) 48, 166, 211
BsuRI GGCC 3 cut(s) 162, 245, 463
BtgZI GCGATG 1 cut(s) 318
BtsCI GGATG 2 cut(s) 208, 347
BtsI GCAGTG 3 cut(s) 71, 436, 448
BtsIMutI CAGTG 4 cut(s) 71, 436, 448, 475
Cac8I GCNNGC 3 cut(s) 44, 222, 229
CfoI GCGC 3 cut(s) 53, 61, 91
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 1 cut(s) 170
Cfr9I CCCGGG 1 cut(s) 173
CviAII CATG 2 cut(s) 344, 371
DdeI CTNAG 1 cut(s) 183
DpnI GATC 1 cut(s) 452
DpnII GATC 1 cut(s) 450
Eam1104I CTCTTC 1 cut(s) 452
EarI CTCTTC 1 cut(s) 452
EciI GGCGGA 1 cut(s) 47
Eco130I CCWWGG 1 cut(s) 246
Eco147I AGGCCT 1 cut(s) 463
Eco47I GGWCC 1 cut(s) 170
Eco88I CYCGRG 1 cut(s) 173
EcoRII CCWGG 1 cut(s) 308
EcoT14I CCWWGG 1 cut(s) 246
EcoT22I ATGCAT 1 cut(s) 349
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 2 cut(s) 347, 374
FaiI YATR 6 cut(s) 191, 287, 345, 372, 378, 449
FaqI GGGAC 1 cut(s) 156
FatI CATG 2 cut(s) 343, 370
Fnu4HI GCNGC 4 cut(s) 30, 62, 180, 225
FokI GGATG 2 cut(s) 215, 334
Fsp4HI GCNGC 4 cut(s) 30, 62, 180, 225
GlaI GCGC 3 cut(s) 52, 60, 90
GluI GCNGC 4 cut(s) 30, 62, 180, 225
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 3 cut(s) 162, 245, 463
HapII CCGG 2 cut(s) 168, 174
HhaI GCGC 3 cut(s) 53, 61, 91
Hin1II CATG 2 cut(s) 347, 374
Hin6I GCGC 3 cut(s) 51, 59, 89
HinP1I GCGC 3 cut(s) 51, 59, 89
HpaII CCGG 2 cut(s) 168, 174
HphI GGTGA 1 cut(s) 304
Hpy166II GTNNAC 2 cut(s) 69, 130
Hpy188III TCNNGA 1 cut(s) 454
Hpy8I GTNNAC 2 cut(s) 69, 130
Hpy99I CGWCG 2 cut(s) 158, 161
HpyAV CCTTC 2 cut(s) 235, 293
HpyCH4III ACNGT 1 cut(s) 73
HpyCH4V TGCA 3 cut(s) 64, 347, 441
HpyF10VI GCNNNNNNNGC 3 cut(s) 48, 115, 431
HpyF3I CTNAG 1 cut(s) 183
Hsp92II CATG 2 cut(s) 347, 374
HspAI GCGC 3 cut(s) 51, 59, 89
Kzo9I GATC 1 cut(s) 450
LmnI GCTCC 3 cut(s) 76, 104, 380
LpnPI CCDG 5 cut(s) 181, 187, 206, 295, 322
Lsp1109I GCAGC 3 cut(s) 48, 166, 211
LweI GCATC 3 cut(s) 193, 295, 356
MaeIII GTNAC 1 cut(s) 413
MalI GATC 1 cut(s) 452
MboI GATC 1 cut(s) 450
MboII GAAGA 3 cut(s) 76, 226, 469
MhlI GDGCHC 2 cut(s) 48, 427
MmeI TCCRAC 1 cut(s) 126
MnlI CCTC 6 cut(s) 160, 173, 178, 283, 319, 453
Mph1103I ATGCAT 1 cut(s) 349
MslI CAYNNNNRTG 3 cut(s) 290, 375, 441
MspI CCGG 2 cut(s) 168, 174
MspR9I CCNGG 3 cut(s) 174, 175, 310
MvaI CCWGG 1 cut(s) 310
MvnI CGCG 3 cut(s) 59, 89, 231
MwoI GCNNNNNNNGC 3 cut(s) 48, 115, 431
NciI CCSGG 2 cut(s) 174, 175
NdeII GATC 1 cut(s) 450
NlaIII CATG 2 cut(s) 347, 374
NlaIV GGNNCC 3 cut(s) 100, 172, 382
NmuCI GTSAC 1 cut(s) 413
NsiI ATGCAT 1 cut(s) 349
OliI CACNNNNGTG 1 cut(s) 441
PceI AGGCCT 1 cut(s) 463
PkrI GCNGC 4 cut(s) 31, 63, 181, 226
Psp6I CCWGG 1 cut(s) 308
PspGI CCWGG 1 cut(s) 308
PspN4I GGNNCC 3 cut(s) 100, 172, 382
PspPI GGNCC 1 cut(s) 170
PstI CTGCAG 2 cut(s) 66, 443
RseI CAYNNNNRTG 3 cut(s) 290, 375, 441
SatI GCNGC 4 cut(s) 30, 62, 180, 225
Sau3AI GATC 1 cut(s) 450
Sau96I GGNCC 1 cut(s) 170
ScrFI CCNGG 3 cut(s) 174, 175, 310
SduI GDGCHC 2 cut(s) 48, 427
SetI ASST 3 cut(s) 120, 189, 294
SfaNI GCATC 3 cut(s) 193, 295, 356
SfcI CTRYAG 2 cut(s) 62, 439
SinI GGWCC 1 cut(s) 170
SmaI CCCGGG 1 cut(s) 175
SmiMI CAYNNNNRTG 3 cut(s) 290, 375, 441
SseBI AGGCCT 1 cut(s) 463
SsiI CCGC 3 cut(s) 29, 32, 395
StuI AGGCCT 1 cut(s) 463
StyD4I CCNGG 3 cut(s) 172, 173, 308
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 1 cut(s) 73
TauI GCSGC 1 cut(s) 32
TscAI CASTG 3 cut(s) 71, 443, 448
TseFI GTSAC 1 cut(s) 413
TseI GCWGC 3 cut(s) 61, 179, 224
Tsp45I GTSAC 1 cut(s) 413
TspGWI ACGGA 2 cut(s) 142, 340
TspMI CCCGGG 1 cut(s) 173
TspRI CASTG 3 cut(s) 71, 443, 448
VpaK11BI GGWCC 1 cut(s) 170
XmaI CCCGGG 1 cut(s) 173
Zsp2I ATGCAT 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.