MD03G1287100.v1.1

Universal stress protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003052
Physical Location & Seq
Forward (+)
36630655 .. 36632754
2100 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1287100.v1.1.491

Sequence Viewer

Length: 486 bp
ATGGCGGCAGAGAAGCAAGTGATGGTGATCGGGGCGGACGACAGCGAGGAGAGCCATTACGCTCTGGAATGGACTTTGGATCACTTCTTCAAGCCTTTAGGCGGCGACACAGCTCCGTTCAAGCTCATCATCGTCTACGCCAAGCCATCGGTTTCCGCCGTCGTTGGCTTCGCCGGCCCCGGAGCGGCGGAGGTTCTGCCGATCGTGGACTCTGATTTGAAGAAGATGGCTGCCCGTGTAACTGAGAAGGCCAAGGAATTTTGCGCTTCTAAATCGGTGACCGATGTGGTCGCGGAGGTTGTGGAAGGAGATGCTAGGAACGTACTGTGTGAGGCTGTTGAAAGACACCACGCATCCATCTTGGTTGTGGGAAGTCATGGCTATGGTGCCATCAAAAGGGCGGTTCTGGGAAGCGTAAGCGACTACTGCACTCATCACGCTCACTGCACCGTGATGATTGTGAAGAAGCCTAAAATCAAGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

162

Amino Acids

17.25

Weight (kDa)

6.49

Isoelectric Point (pI)

24.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 8 - 155 6.4e-24 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 386
AccBSI CCGCTC 1 cut(s) 185
AccI GTMKAC 1 cut(s) 135
AccII CGCG 1 cut(s) 293
AciI CCGC 8 cut(s) 5, 35, 102, 156, 185, 188, 293, 401
AclWI GGATC 1 cut(s) 87
AcsI RAATTY 1 cut(s) 257
AfaI GTAC 1 cut(s) 324
AfiI CCNNNNNNNGG 3 cut(s) 101, 184, 396
AgsI TTSAA 4 cut(s) 91, 121, 220, 341
AluBI AGCT 2 cut(s) 113, 124
AluI AGCT 2 cut(s) 113, 124
AlwI GGATC 1 cut(s) 87
AoxI GGCC 2 cut(s) 175, 249
ApeKI GCWGC 1 cut(s) 230
ApoI RAATTY 1 cut(s) 257
AspLEI GCGC 1 cut(s) 266
AspS9I GGNCC 1 cut(s) 176
AsuC2I CCSGG 1 cut(s) 180
AsuHPI GGTGA 2 cut(s) 37, 289
BanI GGYRCC 1 cut(s) 386
BbvI GCAGC 1 cut(s) 217
BccI CCATC 5 cut(s) 16, 154, 220, 365, 398
BceAI ACGGC 1 cut(s) 143
BcnI CCSGG 1 cut(s) 180
BfaI CTAG 1 cut(s) 315
BisI GCNGC 4 cut(s) 6, 103, 186, 231
BlsI GCNGC 4 cut(s) 7, 104, 187, 232
Bme1390I CCNGG 1 cut(s) 180
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 2 cut(s) 178, 388
BmrFI CCNGG 1 cut(s) 180
BmsI GCATC 2 cut(s) 301, 362
BpuMI CCSGG 1 cut(s) 180
BsaBI GATNNNNATC 1 cut(s) 26
BsaJI CCNNGG 2 cut(s) 178, 252
Bsc4I CCNNNNNNNGG 3 cut(s) 101, 184, 396
Bse118I RCCGGY 1 cut(s) 173
Bse8I GATNNNNATC 1 cut(s) 26
BseDI CCNNGG 2 cut(s) 178, 252
BseGI GGATG 1 cut(s) 353
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 3 cut(s) 101, 184, 396
BseMII CTCAG 1 cut(s) 234
BseRI GAGGAG 1 cut(s) 62
BseXI GCAGC 1 cut(s) 217
BsgI GTGCAG 2 cut(s) 412, 430
Bsh1236I CGCG 1 cut(s) 293
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 2 cut(s) 177, 251
BshNI GGYRCC 1 cut(s) 386
BsiEI CGRYCG 1 cut(s) 204
BsiSI CCGG 2 cut(s) 174, 180
BslI CCNNNNNNNGG 3 cut(s) 101, 184, 396
BsnI GGCC 2 cut(s) 177, 251
Bsp143I GATC 3 cut(s) 27, 79, 201
BspACI CCGC 8 cut(s) 5, 35, 102, 156, 185, 188, 293, 401
BspANI GGCC 2 cut(s) 177, 251
BspCNI CTCAG 1 cut(s) 235
BspFNI CGCG 1 cut(s) 293
BspLI GGNNCC 2 cut(s) 178, 388
BspPI GGATC 1 cut(s) 87
BspT107I GGYRCC 1 cut(s) 386
BsrBI CCGCTC 1 cut(s) 185
BsrFI RCCGGY 1 cut(s) 173
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 2 cut(s) 178, 252
BssMI GATC 3 cut(s) 27, 79, 201
BssT1I CCWWGG 1 cut(s) 252
Bst4CI ACNGT 2 cut(s) 327, 451
BstC8I GCNNGC 1 cut(s) 175
BstDEI CTNAG 1 cut(s) 243
BstEII GGTNACC 1 cut(s) 277
BstF5I GGATG 1 cut(s) 353
BstFNI CGCG 1 cut(s) 293
BstHHI GCGC 1 cut(s) 266
BstKTI GATC 3 cut(s) 30, 82, 204
BstMBI GATC 3 cut(s) 27, 79, 201
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 3 cut(s) 51, 174, 426
BstPI GGTNACC 1 cut(s) 277
BstSCI CCNGG 1 cut(s) 178
BstUI CGCG 1 cut(s) 293
BstV1I GCAGC 1 cut(s) 217
BsuRI GGCC 2 cut(s) 177, 251
BtsCI GGATG 1 cut(s) 353
BtsI GCAGTG 1 cut(s) 442
BtsIMutI CAGTG 2 cut(s) 442, 481
Cac8I GCNNGC 1 cut(s) 175
CfoI GCGC 1 cut(s) 266
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 1 cut(s) 176
Csp6I GTAC 1 cut(s) 323
CviAII CATG 1 cut(s) 377
CviQI GTAC 1 cut(s) 323
DdeI CTNAG 1 cut(s) 243
DpnI GATC 3 cut(s) 29, 81, 203
DpnII GATC 3 cut(s) 27, 79, 201
EciI GGCGGA 3 cut(s) 50, 145, 203
Eco130I CCWWGG 1 cut(s) 252
Eco91I GGTNACC 1 cut(s) 277
EcoO65I GGTNACC 1 cut(s) 277
EcoT14I CCWWGG 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 1 cut(s) 380
FaiI YATR 2 cut(s) 378, 384
FatI CATG 1 cut(s) 376
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 4 cut(s) 6, 103, 186, 231
FokI GGATG 1 cut(s) 340
Fsp4HI GCNGC 4 cut(s) 6, 103, 186, 231
FspBI CTAG 1 cut(s) 315
GlaI GCGC 1 cut(s) 265
GluI GCNGC 4 cut(s) 6, 103, 186, 231
HaeIII GGCC 2 cut(s) 177, 251
HapII CCGG 2 cut(s) 174, 180
HhaI GCGC 1 cut(s) 266
Hin1II CATG 1 cut(s) 380
Hin6I GCGC 1 cut(s) 264
HinP1I GCGC 1 cut(s) 264
HinfI GANTC 1 cut(s) 209
HpaII CCGG 2 cut(s) 174, 180
HphI GGTGA 2 cut(s) 37, 289
Hpy166II GTNNAC 2 cut(s) 136, 208
Hpy188I TCNGA 1 cut(s) 214
Hpy188III TCNNGA 1 cut(s) 65
Hpy8I GTNNAC 2 cut(s) 136, 208
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 2 cut(s) 241, 299
HpyCH4III ACNGT 2 cut(s) 327, 451
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 2 cut(s) 429, 447
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 174, 426
HpyF3I CTNAG 1 cut(s) 243
HpySE526I ACGT 1 cut(s) 321
Hsp92II CATG 1 cut(s) 380
HspAI GCGC 1 cut(s) 264
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
Kzo9I GATC 3 cut(s) 27, 79, 201
LmnI GCTCC 2 cut(s) 118, 182
LpnPI CCDG 4 cut(s) 50, 187, 193, 392
Lsp1109I GCAGC 1 cut(s) 217
LweI GCATC 2 cut(s) 301, 362
MaeI CTAG 1 cut(s) 315
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 2 cut(s) 238, 277
MalI GATC 3 cut(s) 29, 81, 203
MbiI CCGCTC 1 cut(s) 185
MboI GATC 3 cut(s) 27, 79, 201
MboII GAAGA 4 cut(s) 79, 232, 235, 475
MluCI AATT 1 cut(s) 257
MlyI GAGTC 1 cut(s) 203
MnlI CCTC 4 cut(s) 40, 184, 289, 325
MroNI GCCGGC 1 cut(s) 173
MslI CAYNNNNRTG 2 cut(s) 381, 452
MspI CCGG 2 cut(s) 174, 180
MspR9I CCNGG 1 cut(s) 180
MvnI CGCG 1 cut(s) 293
MwoI GCNNNNNNNGC 3 cut(s) 51, 174, 426
NaeI GCCGGC 1 cut(s) 175
NciI CCSGG 1 cut(s) 180
NdeII GATC 3 cut(s) 27, 79, 201
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 1 cut(s) 380
NlaIV GGNNCC 2 cut(s) 178, 388
NmuCI GTSAC 1 cut(s) 277
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PdiI GCCGGC 1 cut(s) 175
PkrI GCNGC 4 cut(s) 7, 104, 187, 232
Ple19I CGATCG 1 cut(s) 204
PleI GAGTC 1 cut(s) 203
PpsI GAGTC 1 cut(s) 203
PspEI GGTNACC 1 cut(s) 277
PspN4I GGNNCC 2 cut(s) 178, 388
PspPI GGNCC 1 cut(s) 176
PvuI CGATCG 1 cut(s) 204
RsaI GTAC 1 cut(s) 324
RsaNI GTAC 1 cut(s) 323
RseI CAYNNNNRTG 2 cut(s) 381, 452
SatI GCNGC 4 cut(s) 6, 103, 186, 231
Sau3AI GATC 3 cut(s) 27, 79, 201
Sau96I GGNCC 1 cut(s) 176
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 1 cut(s) 180
SetI ASST 5 cut(s) 115, 126, 195, 300, 324
SfaNI GCATC 2 cut(s) 301, 362
SmiMI CAYNNNNRTG 2 cut(s) 381, 452
Sse9I AATT 1 cut(s) 257
SsiI CCGC 8 cut(s) 5, 35, 102, 156, 185, 188, 293, 401
SspMI CTAG 1 cut(s) 315
StyD4I CCNGG 1 cut(s) 178
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 2 cut(s) 327, 451
TaiI ACGT 1 cut(s) 324
TaqII GACCGA 1 cut(s) 296
TasI AATT 1 cut(s) 257
TauI GCSGC 3 cut(s) 8, 105, 188
TscAI CASTG 1 cut(s) 449
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 1 cut(s) 230
Tsp45I GTSAC 1 cut(s) 277
TspGWI ACGGA 1 cut(s) 105
TspRI CASTG 1 cut(s) 449
XapI RAATTY 1 cut(s) 257
XcmI CCANNNNNNNNNTGG 1 cut(s) 364
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.