FvH4_4g18260

Universal stress protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003052
Physical Location & Seq
Forward (+)
22210779 .. 22211805
1027 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g18260.t1

Sequence Viewer

Length: 492 bp
ATGGCGGAAAGCGCAAAGCCACTGATGCTAGTTGCTATCGACGACAGTGAGCACAGCTACTATGCATTGGACTGGACTCTGGACCATTTCTTTGTCGACGGGGCCAGCCATCTTTTCCGGCTCGCCCTCGTTCACGCCAGGGCATCTCCGGCTTCTGCTCTTGGAGTCGCCGGAGTTGGCTCTGGCGACTTCCTGAATTCGTTGATTTCTGATATGAAGAAATCGGCTAATAAGACCGTCGAAAAGGCCAAGGAATTGTGCACCAAGAAAAAGGTGGAGAATGTGCAAGTGGAAGTTATGGAAGGTGATCCAAGAAACGTTATGCCTGAAGCCGTGGATAAACACGGTGCAGCTCTCTTGGTTCTCGGCAGTCATGGTTATGGAGCGGTAAAGAGGGCAGTTCTGGGGAGTGTAAGCGACCACTGTGCTCATCATGCTAACTGTTCAGTGATGATTGTGAAGAGGCCAATGAACAAGCCTGCTGCCAAGTAG

Protein Analysis

164

Amino Acids

17.43

Weight (kDa)

7.79

Isoelectric Point (pI)

22.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 9 - 154 1.7e-24 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 386
AccI GTMKAC 1 cut(s) 96
AciI CCGC 2 cut(s) 5, 386
AclI AACGTT 1 cut(s) 318
AclWI GGATC 1 cut(s) 302
AcsI RAATTY 1 cut(s) 196
AcuI CTGAAG 1 cut(s) 348
AjnI CCWGG 1 cut(s) 137
AluBI AGCT 2 cut(s) 57, 353
AluI AGCT 2 cut(s) 57, 353
Alw21I GWGCWC 3 cut(s) 54, 263, 430
Alw44I GTGCAC 1 cut(s) 259
AlwI GGATC 1 cut(s) 302
AoxI GGCC 3 cut(s) 102, 246, 464
ApaLI GTGCAC 1 cut(s) 259
ApeKI GCWGC 2 cut(s) 350, 482
ApoI RAATTY 1 cut(s) 196
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
AspLEI GCGC 1 cut(s) 14
AspS9I GGNCC 2 cut(s) 82, 102
AsuHPI GGTGA 1 cut(s) 317
AvaII GGWCC 1 cut(s) 82
BaeGI GKGCMC 1 cut(s) 263
Bbv12I GWGCWC 3 cut(s) 54, 263, 430
BbvI GCAGC 2 cut(s) 362, 469
BccI CCATC 1 cut(s) 117
BceAI ACGGC 1 cut(s) 317
BcgI CGANNNNNNTGC 2 cut(s) 407, 441
BciT130I CCWGG 1 cut(s) 139
BfaI CTAG 1 cut(s) 29
BisI GCNGC 2 cut(s) 351, 483
BlsI GCNGC 2 cut(s) 352, 484
Bme1390I CCNGG 1 cut(s) 139
Bme18I GGWCC 1 cut(s) 82
BmgT120I GGNCC 2 cut(s) 82, 102
BmiI GGNNCC 1 cut(s) 103
BmrFI CCNGG 1 cut(s) 139
BmsI GCATC 2 cut(s) 15, 152
BsaJI CCNNGG 3 cut(s) 138, 249, 333
Bse1I ACTGG 1 cut(s) 77
BseBI CCWGG 1 cut(s) 139
BseDI CCNNGG 3 cut(s) 138, 249, 333
BseNI ACTGG 1 cut(s) 77
BseSI GKGCMC 1 cut(s) 263
BseXI GCAGC 2 cut(s) 362, 469
BsgI GTGCAG 1 cut(s) 369
BshFI GGCC 3 cut(s) 104, 248, 466
BsiHKAI GWGCWC 3 cut(s) 54, 263, 430
BsiSI CCGG 3 cut(s) 118, 149, 171
BsnI GGCC 3 cut(s) 104, 248, 466
Bsp1286I GDGCHC 3 cut(s) 54, 263, 430
Bsp143I GATC 1 cut(s) 307
BspACI CCGC 2 cut(s) 5, 386
BspANI GGCC 3 cut(s) 104, 248, 466
BspLI GGNNCC 1 cut(s) 103
BspPI GGATC 1 cut(s) 302
BsrBI CCGCTC 1 cut(s) 386
BsrI ACTGG 1 cut(s) 77
BssECI CCNNGG 3 cut(s) 138, 249, 333
BssMI GATC 1 cut(s) 307
BssT1I CCWWGG 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 139
Bst4CI ACNGT 5 cut(s) 47, 238, 347, 425, 443
Bst6I CTCTTC 1 cut(s) 455
BstC8I GCNNGC 3 cut(s) 106, 123, 480
BstDSI CCRYGG 1 cut(s) 333
BstHHI GCGC 1 cut(s) 14
BstKTI GATC 1 cut(s) 310
BstMBI GATC 1 cut(s) 307
BstMWI GCNNNNNNNGC 4 cut(s) 11, 25, 149, 434
BstNI CCWGG 1 cut(s) 139
BstSCI CCNGG 1 cut(s) 137
BstSLI GKGCMC 1 cut(s) 263
BstV1I GCAGC 2 cut(s) 362, 469
BsuRI GGCC 3 cut(s) 104, 248, 466
BtgI CCRYGG 1 cut(s) 333
BtsIMutI CAGTG 4 cut(s) 20, 52, 421, 453
Cac8I GCNNGC 3 cut(s) 106, 123, 480
CfoI GCGC 1 cut(s) 14
Cfr13I GGNCC 2 cut(s) 82, 102
CviAII CATG 2 cut(s) 374, 434
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
Eam1104I CTCTTC 1 cut(s) 455
EarI CTCTTC 1 cut(s) 455
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 82
Eco57I CTGAAG 1 cut(s) 348
EcoRI GAATTC 1 cut(s) 196
EcoRII CCWGG 1 cut(s) 137
EcoT14I CCWWGG 1 cut(s) 249
EcoT22I ATGCAT 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 2 cut(s) 377, 437
FaiI YATR 7 cut(s) 63, 215, 299, 323, 375, 381, 435
FatI CATG 2 cut(s) 373, 433
FblI GTMKAC 1 cut(s) 96
Fnu4HI GCNGC 2 cut(s) 351, 483
Fsp4HI GCNGC 2 cut(s) 351, 483
FspBI CTAG 1 cut(s) 29
GlaI GCGC 1 cut(s) 13
GluI GCNGC 2 cut(s) 351, 483
HaeIII GGCC 3 cut(s) 104, 248, 466
HapII CCGG 3 cut(s) 118, 149, 171
HhaI GCGC 1 cut(s) 14
Hin1II CATG 2 cut(s) 377, 437
Hin6I GCGC 1 cut(s) 12
HinP1I GCGC 1 cut(s) 12
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HinfI GANTC 2 cut(s) 76, 165
HpaII CCGG 3 cut(s) 118, 149, 171
HphI GGTGA 1 cut(s) 317
Hpy166II GTNNAC 3 cut(s) 97, 133, 261
Hpy188I TCNGA 1 cut(s) 211
Hpy188III TCNNGA 2 cut(s) 80, 193
Hpy8I GTNNAC 3 cut(s) 97, 133, 261
Hpy99I CGWCG 3 cut(s) 44, 101, 242
HpyAV CCTTC 1 cut(s) 296
HpyCH4III ACNGT 5 cut(s) 47, 238, 347, 425, 443
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 4 cut(s) 65, 261, 286, 350
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 25, 149, 434
HpySE526I ACGT 1 cut(s) 318
Hsp92II CATG 2 cut(s) 377, 437
HspAI GCGC 1 cut(s) 12
Kzo9I GATC 1 cut(s) 307
LmnI GCTCC 1 cut(s) 383
Lsp1109I GCAGC 2 cut(s) 362, 469
LweI GCATC 2 cut(s) 15, 152
MaeI CTAG 1 cut(s) 29
MaeII ACGT 1 cut(s) 318
MalI GATC 1 cut(s) 309
MbiI CCGCTC 1 cut(s) 386
MboI GATC 1 cut(s) 307
MboII GAAGA 2 cut(s) 229, 472
MhlI GDGCHC 3 cut(s) 54, 263, 430
MluCI AATT 2 cut(s) 196, 254
MlyI GAGTC 2 cut(s) 70, 174
MnlI CCTC 3 cut(s) 137, 387, 456
Mph1103I ATGCAT 1 cut(s) 67
MslI CAYNNNNRTG 1 cut(s) 378
MspI CCGG 3 cut(s) 118, 149, 171
MspR9I CCNGG 1 cut(s) 139
MvaI CCWGG 1 cut(s) 139
MwoI GCNNNNNNNGC 4 cut(s) 11, 25, 149, 434
NdeII GATC 1 cut(s) 307
NlaIII CATG 2 cut(s) 377, 437
NlaIV GGNNCC 1 cut(s) 103
NmeAIII GCCGAG 1 cut(s) 345
NsiI ATGCAT 1 cut(s) 67
PkrI GCNGC 2 cut(s) 352, 484
PleI GAGTC 2 cut(s) 70, 173
PpsI GAGTC 2 cut(s) 70, 173
Psp1406I AACGTT 1 cut(s) 318
Psp6I CCWGG 1 cut(s) 137
PspGI CCWGG 1 cut(s) 137
PspN4I GGNNCC 1 cut(s) 103
PspPI GGNCC 2 cut(s) 82, 102
RseI CAYNNNNRTG 1 cut(s) 378
SalI GTCGAC 1 cut(s) 95
SatI GCNGC 2 cut(s) 351, 483
Sau3AI GATC 1 cut(s) 307
Sau96I GGNCC 2 cut(s) 82, 102
SchI GAGTC 2 cut(s) 70, 174
ScrFI CCNGG 1 cut(s) 139
SduI GDGCHC 3 cut(s) 54, 263, 430
SetI ASST 5 cut(s) 59, 276, 307, 321, 355
SfaNI GCATC 2 cut(s) 15, 152
SinI GGWCC 1 cut(s) 82
SmiMI CAYNNNNRTG 1 cut(s) 378
Sse9I AATT 2 cut(s) 196, 254
SsiI CCGC 2 cut(s) 5, 386
SspMI CTAG 1 cut(s) 29
StyD4I CCNGG 1 cut(s) 137
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 5 cut(s) 47, 238, 347, 425, 443
TaiI ACGT 1 cut(s) 321
TaqI TCGA 3 cut(s) 39, 96, 240
TasI AATT 2 cut(s) 196, 254
TscAI CASTG 4 cut(s) 27, 52, 428, 453
TseI GCWGC 2 cut(s) 350, 482
TspDTI ATGAA 2 cut(s) 230, 485
TspRI CASTG 4 cut(s) 27, 52, 428, 453
VneI GTGCAC 1 cut(s) 259
VpaK11BI GGWCC 1 cut(s) 82
XapI RAATTY 1 cut(s) 196
XcmI CCANNNNNNNNNTGG 1 cut(s) 271
XmiI GTMKAC 1 cut(s) 96
XspI CTAG 1 cut(s) 29
Zsp2I ATGCAT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.