MD03G1287300.v1.1

Universal stress protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003052
Physical Location & Seq
Forward (+)
36637560 .. 36643910
6351 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1287300.v1.1.491

Sequence Viewer

Length: 486 bp
ATGGCGGCAGAGAAGCAAGTGATGGTGATCGGGGCGGACGACAGCGAGGAGAGCCACTACGCTCTGGAATGGACTTTGGATCACTTCTTCAAGCCTTTCGGCGGCGACACTGCTCCGTTCAAGCTCATCATCGTCCACGCCAAGCCATCGGTTTCCTCCGTCGTTGGCTTCGTCGGCGCCGCAGGGGCGGAGATTCTGCCGATCGTGGACGTTGATTTGAAGAAGATGGCTGCCCGTGTAACTGAGAAGGCCAAGGAATTTTGCGCTTCTAAATCGGTGACCGATGTGGTCGTGGAGGTTATGGAAGGAGATGCTAGGAACGTTCTGTGTGAGGCTGCTGAAAGACACCACGCATCCATCTTGGTTGTGGGAAGCCATGGCTATGGAGCCATCAAAAGGGCGTTTCTGGGAAGCGTAAGCGACTACTGCGCTCATCAAGCTCACTGCACCGTGATGATCGTGAAGAAGCCTAAAACCAAGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

162

Amino Acids

17.33

Weight (kDa)

6.49

Isoelectric Point (pI)

23.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 8 - 155 3e-24 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 176
AciI CCGC 5 cut(s) 5, 35, 102, 180, 188
AclI AACGTT 1 cut(s) 321
AclWI GGATC 1 cut(s) 87
AcsI RAATTY 1 cut(s) 257
AcyI GRCGYC 1 cut(s) 177
AfiI CCNNNNNNNGG 2 cut(s) 101, 396
AgsI TTSAA 3 cut(s) 91, 121, 220
AluBI AGCT 2 cut(s) 124, 440
AluI AGCT 2 cut(s) 124, 440
AlwI GGATC 1 cut(s) 87
AoxI GGCC 1 cut(s) 249
ApeKI GCWGC 2 cut(s) 230, 335
ApoI RAATTY 1 cut(s) 257
AspLEI GCGC 3 cut(s) 179, 266, 431
AsuHPI GGTGA 2 cut(s) 37, 289
BanI GGYRCC 1 cut(s) 176
BbvI GCAGC 2 cut(s) 217, 322
BccI CCATC 5 cut(s) 16, 154, 220, 365, 398
BfaI CTAG 1 cut(s) 315
BfoI RGCGCY 1 cut(s) 180
BglI GCCNNNNNGGC 1 cut(s) 185
BisI GCNGC 5 cut(s) 6, 103, 180, 231, 336
BlsI GCNGC 5 cut(s) 7, 104, 181, 232, 337
BmiI GGNNCC 2 cut(s) 178, 388
BmsI GCATC 2 cut(s) 301, 362
BsaBI GATNNNNATC 1 cut(s) 26
BsaHI GRCGYC 1 cut(s) 177
BsaJI CCNNGG 2 cut(s) 252, 376
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 396
Bse8I GATNNNNATC 1 cut(s) 26
BseDI CCNNGG 2 cut(s) 252, 376
BseGI GGATG 1 cut(s) 353
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 2 cut(s) 101, 396
BseMII CTCAG 1 cut(s) 234
BseRI GAGGAG 1 cut(s) 62
BseXI GCAGC 2 cut(s) 217, 322
BsgI GTGCAG 1 cut(s) 430
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 1 cut(s) 251
BshNI GGYRCC 1 cut(s) 176
BsiEI CGRYCG 1 cut(s) 204
BslI CCNNNNNNNGG 2 cut(s) 101, 396
BsnI GGCC 1 cut(s) 251
Bsp143I GATC 4 cut(s) 27, 79, 201, 456
Bsp19I CCATGG 1 cut(s) 376
BspACI CCGC 5 cut(s) 5, 35, 102, 180, 188
BspANI GGCC 1 cut(s) 251
BspCNI CTCAG 1 cut(s) 235
BspLI GGNNCC 2 cut(s) 178, 388
BspPI GGATC 1 cut(s) 87
BspT107I GGYRCC 1 cut(s) 176
BssECI CCNNGG 2 cut(s) 252, 376
BssMI GATC 4 cut(s) 27, 79, 201, 456
BssNI GRCGYC 1 cut(s) 177
BssT1I CCWWGG 2 cut(s) 252, 376
Bst4CI ACNGT 1 cut(s) 451
BstACI GRCGYC 1 cut(s) 177
BstDEI CTNAG 1 cut(s) 243
BstDSI CCRYGG 1 cut(s) 376
BstEII GGTNACC 1 cut(s) 277
BstF5I GGATG 1 cut(s) 353
BstH2I RGCGCY 1 cut(s) 180
BstHHI GCGC 3 cut(s) 179, 266, 431
BstKTI GATC 4 cut(s) 30, 82, 204, 459
BstMBI GATC 4 cut(s) 27, 79, 201, 456
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 5 cut(s) 51, 174, 185, 426, 437
BstPI GGTNACC 1 cut(s) 277
BstV1I GCAGC 2 cut(s) 217, 322
BstXI CCANNNNNNTGG 1 cut(s) 383
BsuRI GGCC 1 cut(s) 251
BtgI CCRYGG 1 cut(s) 376
BtsCI GGATG 1 cut(s) 353
BtsI GCAGTG 2 cut(s) 108, 442
BtsIMutI CAGTG 3 cut(s) 108, 442, 481
CfoI GCGC 3 cut(s) 179, 266, 431
CviAII CATG 1 cut(s) 377
DdeI CTNAG 1 cut(s) 243
DinI GGCGCC 1 cut(s) 178
DpnI GATC 4 cut(s) 29, 81, 203, 458
DpnII GATC 4 cut(s) 27, 79, 201, 456
EciI GGCGGA 2 cut(s) 50, 203
Eco130I CCWWGG 2 cut(s) 252, 376
Eco91I GGTNACC 1 cut(s) 277
EcoO65I GGTNACC 1 cut(s) 277
EcoT14I CCWWGG 2 cut(s) 252, 376
EgeI GGCGCC 1 cut(s) 178
EheI GGCGCC 1 cut(s) 178
ErhI CCWWGG 2 cut(s) 252, 376
FaeI CATG 1 cut(s) 380
FaiI YATR 3 cut(s) 302, 378, 384
FatI CATG 1 cut(s) 376
Fnu4HI GCNGC 5 cut(s) 6, 103, 180, 231, 336
FokI GGATG 1 cut(s) 340
Fsp4HI GCNGC 5 cut(s) 6, 103, 180, 231, 336
FspBI CTAG 1 cut(s) 315
GlaI GCGC 3 cut(s) 178, 265, 430
GluI GCNGC 5 cut(s) 6, 103, 180, 231, 336
HaeII RGCGCY 1 cut(s) 180
HaeIII GGCC 1 cut(s) 251
HhaI GCGC 3 cut(s) 179, 266, 431
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 1 cut(s) 380
Hin6I GCGC 3 cut(s) 177, 264, 429
HinP1I GCGC 3 cut(s) 177, 264, 429
HinfI GANTC 1 cut(s) 193
HphI GGTGA 2 cut(s) 37, 289
Hpy166II GTNNAC 2 cut(s) 136, 208
Hpy188III TCNNGA 2 cut(s) 65, 460
Hpy8I GTNNAC 2 cut(s) 136, 208
Hpy99I CGWCG 2 cut(s) 164, 176
HpyAV CCTTC 2 cut(s) 241, 299
HpyCH4III ACNGT 1 cut(s) 451
HpyCH4IV ACGT 2 cut(s) 210, 321
HpyCH4V TGCA 1 cut(s) 447
HpyF10VI GCNNNNNNNGC 5 cut(s) 51, 174, 185, 426, 437
HpyF3I CTNAG 1 cut(s) 243
HpySE526I ACGT 2 cut(s) 210, 321
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 1 cut(s) 380
HspAI GCGC 3 cut(s) 177, 264, 429
KasI GGCGCC 1 cut(s) 176
Kzo9I GATC 4 cut(s) 27, 79, 201, 456
LmnI GCTCC 2 cut(s) 118, 386
LpnPI CCDG 3 cut(s) 50, 168, 392
Lsp1109I GCAGC 2 cut(s) 217, 322
LweI GCATC 2 cut(s) 301, 362
MaeI CTAG 1 cut(s) 315
MaeII ACGT 2 cut(s) 210, 321
MaeIII GTNAC 2 cut(s) 238, 277
MalI GATC 4 cut(s) 29, 81, 203, 458
MboI GATC 4 cut(s) 27, 79, 201, 456
MboII GAAGA 4 cut(s) 79, 232, 235, 475
MluCI AATT 1 cut(s) 257
Mly113I GGCGCC 1 cut(s) 177
MnlI CCTC 4 cut(s) 40, 166, 289, 325
MslI CAYNNNNRTG 2 cut(s) 381, 452
MwoI GCNNNNNNNGC 5 cut(s) 51, 174, 185, 426, 437
NarI GGCGCC 1 cut(s) 177
NcoI CCATGG 1 cut(s) 376
NdeII GATC 4 cut(s) 27, 79, 201, 456
NlaIII CATG 1 cut(s) 380
NlaIV GGNNCC 2 cut(s) 178, 388
NmuCI GTSAC 1 cut(s) 277
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 168
PfeI GAWTC 1 cut(s) 193
PkrI GCNGC 5 cut(s) 7, 104, 181, 232, 337
Ple19I CGATCG 1 cut(s) 204
PluTI GGCGCC 1 cut(s) 180
Psp1406I AACGTT 1 cut(s) 321
PspEI GGTNACC 1 cut(s) 277
PspN4I GGNNCC 2 cut(s) 178, 388
PvuI CGATCG 1 cut(s) 204
RseI CAYNNNNRTG 2 cut(s) 381, 452
SatI GCNGC 5 cut(s) 6, 103, 180, 231, 336
Sau3AI GATC 4 cut(s) 27, 79, 201, 456
SetI ASST 5 cut(s) 126, 213, 300, 324, 442
SfaNI GCATC 2 cut(s) 301, 362
SfoI GGCGCC 1 cut(s) 178
SmiMI CAYNNNNRTG 2 cut(s) 381, 452
Sse9I AATT 1 cut(s) 257
SsiI CCGC 5 cut(s) 5, 35, 102, 180, 188
SspDI GGCGCC 1 cut(s) 176
SspMI CTAG 1 cut(s) 315
StyI CCWWGG 2 cut(s) 252, 376
TaaI ACNGT 1 cut(s) 451
TaiI ACGT 2 cut(s) 213, 324
TaqII GACCGA 1 cut(s) 296
TasI AATT 1 cut(s) 257
TauI GCSGC 3 cut(s) 8, 105, 182
TfiI GAWTC 1 cut(s) 193
TscAI CASTG 2 cut(s) 115, 449
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 2 cut(s) 230, 335
Tsp45I GTSAC 1 cut(s) 277
TspGWI ACGGA 2 cut(s) 105, 148
TspRI CASTG 2 cut(s) 115, 449
XapI RAATTY 1 cut(s) 257
XcmI CCANNNNNNNNNTGG 1 cut(s) 364
XspI CTAG 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.