MD11G1311000.v1.1

Universal stress protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003052
Physical Location & Seq
Reverse (-)
42364041 .. 42365438
1398 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1311000.v1.1.491

Sequence Viewer

Length: 486 bp
ATGGCGGCAGAGAAGCAAGTGATGGTGATCGGGGTGGACGAGAGCGAGCAGAGCCAGTACGCTCTGGAATGGACTTTGGACCACTTCTTCAAGCCCTTCGGCGGCGACAAAGCTCCCTTCAAGCTCATCATTGTCCACGCAAAGGTCCAGGTTTCCGCCGCCGTCAGCTTCGCCGGCCCCGGAGCGGCGGAGATTCTGCCGATCGTGGAAGCTGATTTGAAGAAGACTGCTGCCCACGTAACTGAGAAGGCCAAGGAATTTTGCGCCTCTAAATCGGTGACCGATGTGGTTGTGGAGGTTGTGGAAGGAGATGCTAGGAACGTTCTGTGTGATGCTGTTGAAAAACACCACGCATCCATCTTGGTTGTGGGAAGTCATGGCTATGGAGCCATCAAAAGGGCGGTTCTGGGAAGCGTCAGCGACTACTGCGCCCATCACGCTCACAGCACCGTGATGATCGTGAAGAAGCCTAAAACCAAGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

162

Amino Acids

17.26

Weight (kDa)

6.79

Isoelectric Point (pI)

16.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 8 - 155 9.3e-25 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 185
AciI CCGC 7 cut(s) 5, 102, 156, 159, 185, 188, 401
AclI AACGTT 1 cut(s) 321
AcsI RAATTY 1 cut(s) 257
AfaI GTAC 1 cut(s) 59
AfiI CCNNNNNNNGG 4 cut(s) 101, 142, 184, 396
AgsI TTSAA 4 cut(s) 91, 121, 220, 341
AjnI CCWGG 1 cut(s) 147
AluBI AGCT 4 cut(s) 113, 124, 168, 212
AluI AGCT 4 cut(s) 113, 124, 168, 212
AoxI GGCC 2 cut(s) 175, 249
ApeKI GCWGC 1 cut(s) 230
ApoI RAATTY 1 cut(s) 257
AspLEI GCGC 2 cut(s) 266, 431
AspS9I GGNCC 3 cut(s) 79, 145, 176
AsuC2I CCSGG 1 cut(s) 180
AsuHPI GGTGA 2 cut(s) 37, 289
AvaII GGWCC 2 cut(s) 79, 145
BbsI GAAGAC 1 cut(s) 230
BbvI GCAGC 1 cut(s) 217
BccI CCATC 4 cut(s) 16, 365, 398, 441
BceAI ACGGC 1 cut(s) 146
BciT130I CCWGG 1 cut(s) 149
BcnI CCSGG 1 cut(s) 180
BfaI CTAG 1 cut(s) 315
BisI GCNGC 5 cut(s) 6, 103, 159, 186, 231
BlsI GCNGC 5 cut(s) 7, 104, 160, 187, 232
Bme1390I CCNGG 2 cut(s) 149, 180
Bme18I GGWCC 2 cut(s) 79, 145
BmgT120I GGNCC 3 cut(s) 79, 145, 176
BmiI GGNNCC 2 cut(s) 178, 388
BmrFI CCNGG 2 cut(s) 149, 180
BmsI GCATC 3 cut(s) 301, 322, 362
BpiI GAAGAC 1 cut(s) 230
BpuMI CCSGG 1 cut(s) 180
BsaAI YACGTR 1 cut(s) 238
BsaBI GATNNNNATC 1 cut(s) 26
BsaJI CCNNGG 2 cut(s) 178, 252
Bsc4I CCNNNNNNNGG 4 cut(s) 101, 142, 184, 396
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 1 cut(s) 55
Bse8I GATNNNNATC 1 cut(s) 26
BseBI CCWGG 1 cut(s) 149
BseDI CCNNGG 2 cut(s) 178, 252
BseGI GGATG 1 cut(s) 353
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 4 cut(s) 101, 142, 184, 396
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 1 cut(s) 55
BseXI GCAGC 1 cut(s) 217
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 2 cut(s) 177, 251
BsiEI CGRYCG 1 cut(s) 204
BsiSI CCGG 2 cut(s) 174, 180
BslI CCNNNNNNNGG 4 cut(s) 101, 142, 184, 396
BsnI GGCC 2 cut(s) 177, 251
Bsp143I GATC 3 cut(s) 27, 201, 456
BspACI CCGC 7 cut(s) 5, 102, 156, 159, 185, 188, 401
BspANI GGCC 2 cut(s) 177, 251
BspCNI CTCAG 1 cut(s) 235
BspLI GGNNCC 2 cut(s) 178, 388
BsrBI CCGCTC 1 cut(s) 185
BsrFI RCCGGY 1 cut(s) 173
BsrI ACTGG 1 cut(s) 55
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 2 cut(s) 178, 252
BssMI GATC 3 cut(s) 27, 201, 456
BssT1I CCWWGG 1 cut(s) 252
Bst2UI CCWGG 1 cut(s) 149
Bst4CI ACNGT 1 cut(s) 451
BstBAI YACGTR 1 cut(s) 238
BstC8I GCNNGC 2 cut(s) 47, 175
BstDEI CTNAG 1 cut(s) 243
BstEII GGTNACC 1 cut(s) 277
BstF5I GGATG 1 cut(s) 353
BstHHI GCGC 2 cut(s) 266, 431
BstKTI GATC 3 cut(s) 30, 204, 459
BstMBI GATC 3 cut(s) 27, 201, 456
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 4 cut(s) 51, 174, 426, 437
BstNI CCWGG 1 cut(s) 149
BstPI GGTNACC 1 cut(s) 277
BstSCI CCNGG 2 cut(s) 147, 178
BstV1I GCAGC 1 cut(s) 217
BstV2I GAAGAC 1 cut(s) 230
BsuRI GGCC 2 cut(s) 177, 251
BtsCI GGATG 1 cut(s) 353
Cac8I GCNNGC 2 cut(s) 47, 175
CfoI GCGC 2 cut(s) 266, 431
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 3 cut(s) 79, 145, 176
CseI GACGC 1 cut(s) 403
Csp6I GTAC 1 cut(s) 58
CviAII CATG 1 cut(s) 377
CviQI GTAC 1 cut(s) 58
DdeI CTNAG 1 cut(s) 243
DpnI GATC 3 cut(s) 29, 203, 458
DpnII GATC 3 cut(s) 27, 201, 456
EciI GGCGGA 2 cut(s) 145, 203
Eco130I CCWWGG 1 cut(s) 252
Eco47I GGWCC 2 cut(s) 79, 145
Eco91I GGTNACC 1 cut(s) 277
EcoO65I GGTNACC 1 cut(s) 277
EcoRII CCWGG 1 cut(s) 147
EcoT14I CCWWGG 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 1 cut(s) 380
FaiI YATR 2 cut(s) 378, 384
FatI CATG 1 cut(s) 376
Fnu4HI GCNGC 5 cut(s) 6, 103, 159, 186, 231
FokI GGATG 1 cut(s) 340
Fsp4HI GCNGC 5 cut(s) 6, 103, 159, 186, 231
FspBI CTAG 1 cut(s) 315
GlaI GCGC 2 cut(s) 265, 430
GluI GCNGC 5 cut(s) 6, 103, 159, 186, 231
HaeIII GGCC 2 cut(s) 177, 251
HapII CCGG 2 cut(s) 174, 180
HgaI GACGC 1 cut(s) 403
HhaI GCGC 2 cut(s) 266, 431
Hin1II CATG 1 cut(s) 380
Hin6I GCGC 2 cut(s) 264, 429
HinP1I GCGC 2 cut(s) 264, 429
HinfI GANTC 1 cut(s) 193
HpaII CCGG 2 cut(s) 174, 180
HphI GGTGA 2 cut(s) 37, 289
Hpy166II GTNNAC 2 cut(s) 37, 136
Hpy188III TCNNGA 2 cut(s) 65, 460
Hpy8I GTNNAC 2 cut(s) 37, 136
HpyAV CCTTC 4 cut(s) 106, 127, 241, 299
HpyCH4III ACNGT 1 cut(s) 451
HpyCH4IV ACGT 2 cut(s) 237, 321
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 174, 426, 437
HpyF3I CTNAG 1 cut(s) 243
HpySE526I ACGT 2 cut(s) 237, 321
Hsp92II CATG 1 cut(s) 380
HspAI GCGC 2 cut(s) 264, 429
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
Kzo9I GATC 3 cut(s) 27, 201, 456
LmnI GCTCC 3 cut(s) 118, 182, 386
LpnPI CCDG 7 cut(s) 50, 68, 134, 161, 187, 193, 392
Lsp1109I GCAGC 1 cut(s) 217
LweI GCATC 3 cut(s) 301, 322, 362
MaeI CTAG 1 cut(s) 315
MaeII ACGT 2 cut(s) 237, 321
MaeIII GTNAC 2 cut(s) 238, 277
MalI GATC 3 cut(s) 29, 203, 458
MbiI CCGCTC 1 cut(s) 185
MboI GATC 3 cut(s) 27, 201, 456
MboII GAAGA 4 cut(s) 79, 232, 235, 475
MluCI AATT 1 cut(s) 257
MnlI CCTC 2 cut(s) 277, 289
MroNI GCCGGC 1 cut(s) 173
MslI CAYNNNNRTG 2 cut(s) 381, 452
MspI CCGG 2 cut(s) 174, 180
MspR9I CCNGG 2 cut(s) 149, 180
MvaI CCWGG 1 cut(s) 149
MwoI GCNNNNNNNGC 4 cut(s) 51, 174, 426, 437
NaeI GCCGGC 1 cut(s) 175
NciI CCSGG 1 cut(s) 180
NdeII GATC 3 cut(s) 27, 201, 456
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 1 cut(s) 380
NlaIV GGNNCC 2 cut(s) 178, 388
NmuCI GTSAC 1 cut(s) 277
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 1 cut(s) 193
PkrI GCNGC 5 cut(s) 7, 104, 160, 187, 232
Ple19I CGATCG 1 cut(s) 204
Ppu21I YACGTR 1 cut(s) 238
Psp1406I AACGTT 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 147
PspEI GGTNACC 1 cut(s) 277
PspGI CCWGG 1 cut(s) 147
PspN4I GGNNCC 2 cut(s) 178, 388
PspPI GGNCC 3 cut(s) 79, 145, 176
PvuI CGATCG 1 cut(s) 204
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
RseI CAYNNNNRTG 2 cut(s) 381, 452
SatI GCNGC 5 cut(s) 6, 103, 159, 186, 231
Sau3AI GATC 3 cut(s) 27, 201, 456
Sau96I GGNCC 3 cut(s) 79, 145, 176
ScrFI CCNGG 2 cut(s) 149, 180
SetI ASST 9 cut(s) 115, 126, 147, 153, 170, 214, 240, 300, 324
SfaNI GCATC 3 cut(s) 301, 322, 362
SinI GGWCC 2 cut(s) 79, 145
SmiMI CAYNNNNRTG 2 cut(s) 381, 452
Sse9I AATT 1 cut(s) 257
SsiI CCGC 7 cut(s) 5, 102, 156, 159, 185, 188, 401
SspMI CTAG 1 cut(s) 315
StyD4I CCNGG 2 cut(s) 147, 178
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 1 cut(s) 451
TaiI ACGT 2 cut(s) 240, 324
TaqII GACCGA 1 cut(s) 296
TasI AATT 1 cut(s) 257
TauI GCSGC 4 cut(s) 8, 105, 161, 188
TfiI GAWTC 1 cut(s) 193
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 1 cut(s) 230
Tsp45I GTSAC 1 cut(s) 277
VpaK11BI GGWCC 2 cut(s) 79, 145
XapI RAATTY 1 cut(s) 257
XcmI CCANNNNNNNNNTGG 1 cut(s) 364
XspI CTAG 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.