Rh4AG239900

Universal stress protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003052
Physical Location & Seq
Forward (+)
54524375 .. 54525810
1436 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG239900.1

Sequence Viewer

Length: 420 bp
ATGGCGGAAAGCGCTAAGCCACTGATGCTAATTGCTATCGACGACAGTGAACACAGCTTTTATGCATTGGAGTGGACTCTGGACCATTTGTTTGCTCCCGGGTCCACCCATCCTTTCAAGCTCGGCATCATCCATGCTAGGGCATCTCCCGCTTCTGCTCTCGGAGTCGCCGGAGTTGGCTCTGGGGACTTCTTGAATTCGTTGATTTCTGATCTGAAGAAATCGGCTACTAAGACGGTCGAAAAGGCCAAGGAAATGTGCGTCACGAAGAAGGTGGAAATTGTGCTAGTGGAAGTTATGGAAGGTGATCCGAGAAATGTTATGCCCGAAGCTGTGGATAAACACAAAGCAGCCCTCTTGGTTCTCGGCAGTCATGGTTATGGAGCGGTAAAGAGGTATATGTTTCACTTTAATACGTGA

Protein Analysis

139

Amino Acids

15.05

Weight (kDa)

6.65

Isoelectric Point (pI)

24.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 9 - 135 1.6e-10 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 386
AciI CCGC 3 cut(s) 5, 150, 386
AclWI GGATC 1 cut(s) 302
AcsI RAATTY 1 cut(s) 196
AcuI CTGAAG 1 cut(s) 236
AfeI AGCGCT 1 cut(s) 13
AfiI CCNNNNNNNGG 1 cut(s) 139
AgsI TTSAA 2 cut(s) 118, 196
AluBI AGCT 3 cut(s) 57, 121, 332
AluI AGCT 3 cut(s) 57, 121, 332
AlwI GGATC 1 cut(s) 302
Ama87I CYCGRG 1 cut(s) 98
Aor51HI AGCGCT 1 cut(s) 13
AoxI GGCC 1 cut(s) 246
ApeKI GCWGC 1 cut(s) 350
ApoI RAATTY 1 cut(s) 196
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
AspLEI GCGC 1 cut(s) 14
AspS9I GGNCC 2 cut(s) 82, 102
AsuC2I CCSGG 2 cut(s) 99, 100
AsuHPI GGTGA 1 cut(s) 317
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 2 cut(s) 82, 102
BbvI GCAGC 1 cut(s) 362
BccI CCATC 1 cut(s) 117
BcnI CCSGG 2 cut(s) 99, 100
BfaI CTAG 2 cut(s) 138, 287
BfoI RGCGCY 1 cut(s) 15
BisI GCNGC 1 cut(s) 351
BlpI GCTNAGC 1 cut(s) 15
BlsI GCNGC 1 cut(s) 352
Bme1390I CCNGG 2 cut(s) 99, 100
Bme18I GGWCC 2 cut(s) 82, 102
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 2 cut(s) 82, 102
BmiI GGNNCC 1 cut(s) 103
BmrFI CCNGG 2 cut(s) 99, 100
BmsI GCATC 3 cut(s) 15, 135, 152
Bpu1102I GCTNAGC 1 cut(s) 15
BpuMI CCSGG 2 cut(s) 99, 100
BsaAI YACGTR 1 cut(s) 417
BsaJI CCNNGG 2 cut(s) 98, 249
Bsc4I CCNNNNNNNGG 1 cut(s) 139
BseDI CCNNGG 2 cut(s) 98, 249
BseGI GGATG 2 cut(s) 109, 129
BseLI CCNNNNNNNGG 1 cut(s) 139
BseXI GCAGC 1 cut(s) 362
Bsh1285I CGRYCG 1 cut(s) 240
BshFI GGCC 1 cut(s) 248
BsiEI CGRYCG 1 cut(s) 240
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 2 cut(s) 99, 171
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 1 cut(s) 139
BsmFI GGGAC 1 cut(s) 200
BsnI GGCC 1 cut(s) 248
BsoBI CYCGRG 1 cut(s) 98
Bsp143I GATC 2 cut(s) 211, 307
Bsp1720I GCTNAGC 1 cut(s) 15
BspACI CCGC 3 cut(s) 5, 150, 386
BspANI GGCC 1 cut(s) 248
BspLI GGNNCC 1 cut(s) 103
BspPI GGATC 1 cut(s) 302
BsrBI CCGCTC 1 cut(s) 386
BssECI CCNNGG 2 cut(s) 98, 249
BssMI GATC 2 cut(s) 211, 307
BssT1I CCWWGG 1 cut(s) 249
Bst4CI ACNGT 2 cut(s) 47, 238
BstBAI YACGTR 1 cut(s) 417
BstDEI CTNAG 2 cut(s) 15, 231
BstF5I GGATG 2 cut(s) 109, 129
BstH2I RGCGCY 1 cut(s) 15
BstHHI GCGC 1 cut(s) 14
BstKTI GATC 2 cut(s) 214, 310
BstMBI GATC 2 cut(s) 211, 307
BstMCI CGRYCG 1 cut(s) 240
BstMWI GCNNNNNNNGC 3 cut(s) 11, 25, 149
BstSCI CCNGG 2 cut(s) 97, 98
BstV1I GCAGC 1 cut(s) 362
BsuRI GGCC 1 cut(s) 248
BtsCI GGATG 2 cut(s) 109, 129
BtsIMutI CAGTG 2 cut(s) 20, 52
CfoI GCGC 1 cut(s) 14
Cfr13I GGNCC 2 cut(s) 82, 102
Cfr9I CCCGGG 1 cut(s) 98
CseI GACGC 1 cut(s) 250
CviAII CATG 2 cut(s) 134, 374
CviJI RGCY 8 cut(s) 19, 57, 121, 180, 227, 248, 332, 353
CviKI_1 RGCY 8 cut(s) 19, 57, 121, 180, 227, 248, 332, 353
DdeI CTNAG 2 cut(s) 15, 231
DpnI GATC 2 cut(s) 213, 309
DpnII GATC 2 cut(s) 211, 307
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 249
Eco47I GGWCC 2 cut(s) 82, 102
Eco47III AGCGCT 1 cut(s) 13
Eco57I CTGAAG 1 cut(s) 236
Eco88I CYCGRG 1 cut(s) 98
EcoRI GAATTC 1 cut(s) 196
EcoT14I CCWWGG 1 cut(s) 249
EcoT22I ATGCAT 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 2 cut(s) 137, 377
FaiI YATR 8 cut(s) 63, 135, 299, 323, 375, 381, 399, 401
FaqI GGGAC 1 cut(s) 200
FatI CATG 2 cut(s) 133, 373
FauI CCCGC 1 cut(s) 157
Fnu4HI GCNGC 1 cut(s) 351
FokI GGATG 2 cut(s) 96, 116
Fsp4HI GCNGC 1 cut(s) 351
FspBI CTAG 2 cut(s) 138, 287
GlaI GCGC 1 cut(s) 13
GluI GCNGC 1 cut(s) 351
HaeII RGCGCY 1 cut(s) 15
HaeIII GGCC 1 cut(s) 248
HapII CCGG 2 cut(s) 99, 171
HgaI GACGC 1 cut(s) 250
HhaI GCGC 1 cut(s) 14
Hin1II CATG 2 cut(s) 137, 377
Hin6I GCGC 1 cut(s) 12
HinP1I GCGC 1 cut(s) 12
HinfI GANTC 2 cut(s) 76, 165
HpaII CCGG 2 cut(s) 99, 171
HphI GGTGA 1 cut(s) 317
Hpy166II GTNNAC 3 cut(s) 50, 75, 105
Hpy188I TCNGA 4 cut(s) 164, 211, 216, 312
Hpy188III TCNNGA 3 cut(s) 80, 193, 265
Hpy8I GTNNAC 3 cut(s) 50, 75, 105
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 2 cut(s) 265, 296
HpyCH4III ACNGT 2 cut(s) 47, 238
HpyCH4IV ACGT 1 cut(s) 416
HpyCH4V TGCA 1 cut(s) 65
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 25, 149
HpyF3I CTNAG 2 cut(s) 15, 231
HpySE526I ACGT 1 cut(s) 416
Hsp92II CATG 2 cut(s) 137, 377
HspAI GCGC 1 cut(s) 12
Kzo9I GATC 2 cut(s) 211, 307
LmnI GCTCC 2 cut(s) 100, 383
LpnPI CCDG 4 cut(s) 65, 112, 168, 184
Lsp1109I GCAGC 1 cut(s) 362
LweI GCATC 3 cut(s) 15, 135, 152
MaeI CTAG 2 cut(s) 138, 287
MaeII ACGT 1 cut(s) 416
MaeIII GTNAC 1 cut(s) 262
MalI GATC 2 cut(s) 213, 309
MbiI CCGCTC 1 cut(s) 386
MboI GATC 2 cut(s) 211, 307
MboII GAAGA 2 cut(s) 229, 280
MluCI AATT 3 cut(s) 30, 196, 279
MlyI GAGTC 2 cut(s) 70, 174
MnlI CCTC 2 cut(s) 365, 387
Mph1103I ATGCAT 1 cut(s) 67
MseI TTAA 1 cut(s) 411
MslI CAYNNNNRTG 2 cut(s) 70, 378
MspI CCGG 2 cut(s) 99, 171
MspR9I CCNGG 2 cut(s) 99, 100
MwoI GCNNNNNNNGC 3 cut(s) 11, 25, 149
NciI CCSGG 2 cut(s) 99, 100
NdeII GATC 2 cut(s) 211, 307
NlaIII CATG 2 cut(s) 137, 377
NlaIV GGNNCC 1 cut(s) 103
NmeAIII GCCGAG 2 cut(s) 102, 345
NmuCI GTSAC 1 cut(s) 262
NsiI ATGCAT 1 cut(s) 67
PkrI GCNGC 1 cut(s) 352
PleI GAGTC 2 cut(s) 70, 173
PpsI GAGTC 2 cut(s) 70, 173
Ppu21I YACGTR 1 cut(s) 417
PspN4I GGNNCC 1 cut(s) 103
PspPI GGNCC 2 cut(s) 82, 102
RseI CAYNNNNRTG 2 cut(s) 70, 378
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 1 cut(s) 351
Sau3AI GATC 2 cut(s) 211, 307
Sau96I GGNCC 2 cut(s) 82, 102
SchI GAGTC 2 cut(s) 70, 174
ScrFI CCNGG 2 cut(s) 99, 100
SetI ASST 7 cut(s) 59, 123, 276, 307, 334, 398, 419
SfaNI GCATC 3 cut(s) 15, 135, 152
SinI GGWCC 2 cut(s) 82, 102
SmaI CCCGGG 1 cut(s) 100
SmiMI CAYNNNNRTG 2 cut(s) 70, 378
Sse9I AATT 3 cut(s) 30, 196, 279
SsiI CCGC 3 cut(s) 5, 150, 386
SspMI CTAG 2 cut(s) 138, 287
StyD4I CCNGG 2 cut(s) 97, 98
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 2 cut(s) 47, 238
TaiI ACGT 1 cut(s) 419
TaqI TCGA 2 cut(s) 39, 240
TasI AATT 3 cut(s) 30, 196, 279
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 2 cut(s) 27, 52
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 1 cut(s) 350
Tsp45I GTSAC 1 cut(s) 262
TspMI CCCGGG 1 cut(s) 98
TspRI CASTG 2 cut(s) 27, 52
VpaK11BI GGWCC 2 cut(s) 82, 102
XapI RAATTY 1 cut(s) 196
XmaI CCCGGG 1 cut(s) 98
XspI CTAG 2 cut(s) 138, 287
Zsp2I ATGCAT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.