MD03G1287200.v1.1

Universal stress protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003052
Physical Location & Seq
Forward (+)
36633517 .. 36635337
1821 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1287200.v1.1.491

Sequence Viewer

Length: 486 bp
ATGGCGGCAGGGAAGCAAGTGATGGTGATCGGAGCGGACGACAGCGAGGAGGGCCATTACGCTCTGGAATGGACTTTGGATCACCTCTTCAAGCCTTTGGGCGGCGACACAGCTCCGTTCAAGCTCATCATCGTCCACGCCAAGCCATCGGTTTCCTCCGTCGTTGGCTTCGTCGGCCCCGCAGGGGCGGAGGTTCTGCCGATCGTGGACGCTGATTTGAAGAAGATGGCTGCCCGTGTAACTGAGAGGGCCAAGGAATTTTGCGCTTCTAAATCGGTGACCGATGTGGTCGTGGAGGTTATGGAAGGAGATGCTAGGAACGTTCTGTGTGAGGCTGTTGAAAGACACCACGCATCCATCTTGGTTGTGGGAAGTCATGGCTATGGAGCCATCAAAAGGGCGCTTCTGGGAAGCGTAAGCGACTACTGCGCTCATCACGTTCACTGCACCGTGATGATTGTGAAGAAGCCAAAAACCAAGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

162

Amino Acids

17.21

Weight (kDa)

6.79

Isoelectric Point (pI)

21.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 8 - 155 9.1e-24 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 35
AciI CCGC 5 cut(s) 5, 35, 102, 180, 188
AclI AACGTT 1 cut(s) 321
AclWI GGATC 1 cut(s) 87
AcsI RAATTY 1 cut(s) 257
AfiI CCNNNNNNNGG 3 cut(s) 101, 184, 396
AgsI TTSAA 4 cut(s) 91, 121, 220, 341
AluBI AGCT 2 cut(s) 113, 124
AluI AGCT 2 cut(s) 113, 124
AlwI GGATC 1 cut(s) 87
AoxI GGCC 3 cut(s) 52, 175, 249
ApeKI GCWGC 1 cut(s) 230
ApoI RAATTY 1 cut(s) 257
AspLEI GCGC 3 cut(s) 266, 403, 431
AspS9I GGNCC 3 cut(s) 52, 176, 249
AsuHPI GGTGA 3 cut(s) 37, 74, 289
BbvI GCAGC 1 cut(s) 217
BccI CCATC 5 cut(s) 16, 154, 220, 365, 398
BfaI CTAG 1 cut(s) 315
BfoI RGCGCY 1 cut(s) 404
BisI GCNGC 3 cut(s) 6, 103, 231
BlsI GCNGC 3 cut(s) 7, 104, 232
BmgT120I GGNCC 3 cut(s) 52, 176, 249
BmiI GGNNCC 2 cut(s) 178, 388
BmsI GCATC 2 cut(s) 301, 362
BsaBI GATNNNNATC 1 cut(s) 26
BsaJI CCNNGG 1 cut(s) 252
Bsc4I CCNNNNNNNGG 3 cut(s) 101, 184, 396
Bse8I GATNNNNATC 1 cut(s) 26
BseDI CCNNGG 1 cut(s) 252
BseGI GGATG 1 cut(s) 353
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 3 cut(s) 101, 184, 396
BseMII CTCAG 1 cut(s) 234
BseRI GAGGAG 1 cut(s) 62
BseXI GCAGC 1 cut(s) 217
BsgI GTGCAG 1 cut(s) 430
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 3 cut(s) 54, 177, 251
BsiEI CGRYCG 1 cut(s) 204
BslI CCNNNNNNNGG 3 cut(s) 101, 184, 396
BsnI GGCC 3 cut(s) 54, 177, 251
Bsp143I GATC 3 cut(s) 27, 79, 201
BspACI CCGC 5 cut(s) 5, 35, 102, 180, 188
BspANI GGCC 3 cut(s) 54, 177, 251
BspCNI CTCAG 1 cut(s) 235
BspLI GGNNCC 2 cut(s) 178, 388
BspPI GGATC 1 cut(s) 87
BsrBI CCGCTC 1 cut(s) 35
BssECI CCNNGG 1 cut(s) 252
BssMI GATC 3 cut(s) 27, 79, 201
BssT1I CCWWGG 1 cut(s) 252
Bst4CI ACNGT 1 cut(s) 451
Bst6I CTCTTC 1 cut(s) 92
BstDEI CTNAG 1 cut(s) 243
BstEII GGTNACC 1 cut(s) 277
BstF5I GGATG 1 cut(s) 353
BstH2I RGCGCY 1 cut(s) 404
BstHHI GCGC 3 cut(s) 266, 403, 431
BstKTI GATC 3 cut(s) 30, 82, 204
BstMBI GATC 3 cut(s) 27, 79, 201
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 3 cut(s) 51, 174, 426
BstPI GGTNACC 1 cut(s) 277
BstV1I GCAGC 1 cut(s) 217
BsuRI GGCC 3 cut(s) 54, 177, 251
BtsCI GGATG 1 cut(s) 353
BtsI GCAGTG 1 cut(s) 442
BtsIMutI CAGTG 2 cut(s) 442, 481
CfoI GCGC 3 cut(s) 266, 403, 431
Cfr13I GGNCC 3 cut(s) 52, 176, 249
CseI GACGC 1 cut(s) 218
CviAII CATG 1 cut(s) 377
DdeI CTNAG 1 cut(s) 243
DpnI GATC 3 cut(s) 29, 81, 203
DpnII GATC 3 cut(s) 27, 79, 201
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
EciI GGCGGA 1 cut(s) 203
Eco130I CCWWGG 1 cut(s) 252
Eco91I GGTNACC 1 cut(s) 277
EcoO65I GGTNACC 1 cut(s) 277
EcoT14I CCWWGG 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 1 cut(s) 380
FaiI YATR 3 cut(s) 302, 378, 384
FatI CATG 1 cut(s) 376
FauI CCCGC 1 cut(s) 187
Fnu4HI GCNGC 3 cut(s) 6, 103, 231
FokI GGATG 1 cut(s) 340
Fsp4HI GCNGC 3 cut(s) 6, 103, 231
FspBI CTAG 1 cut(s) 315
GlaI GCGC 3 cut(s) 265, 402, 430
GluI GCNGC 3 cut(s) 6, 103, 231
HaeII RGCGCY 1 cut(s) 404
HaeIII GGCC 3 cut(s) 54, 177, 251
HgaI GACGC 1 cut(s) 218
HhaI GCGC 3 cut(s) 266, 403, 431
Hin1II CATG 1 cut(s) 380
Hin6I GCGC 3 cut(s) 264, 401, 429
HinP1I GCGC 3 cut(s) 264, 401, 429
HphI GGTGA 3 cut(s) 37, 74, 289
Hpy166II GTNNAC 3 cut(s) 136, 208, 442
Hpy188I TCNGA 1 cut(s) 32
Hpy188III TCNNGA 1 cut(s) 65
Hpy8I GTNNAC 3 cut(s) 136, 208, 442
Hpy99I CGWCG 2 cut(s) 164, 176
HpyAV CCTTC 1 cut(s) 299
HpyCH4III ACNGT 1 cut(s) 451
HpyCH4IV ACGT 2 cut(s) 321, 438
HpyCH4V TGCA 1 cut(s) 447
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 174, 426
HpyF3I CTNAG 1 cut(s) 243
HpySE526I ACGT 2 cut(s) 321, 438
Hsp92II CATG 1 cut(s) 380
HspAI GCGC 3 cut(s) 264, 401, 429
Kzo9I GATC 3 cut(s) 27, 79, 201
LmnI GCTCC 3 cut(s) 32, 118, 386
LpnPI CCDG 3 cut(s) 50, 168, 392
Lsp1109I GCAGC 1 cut(s) 217
LweI GCATC 2 cut(s) 301, 362
MaeI CTAG 1 cut(s) 315
MaeII ACGT 2 cut(s) 321, 438
MaeIII GTNAC 2 cut(s) 238, 277
MalI GATC 3 cut(s) 29, 81, 203
MbiI CCGCTC 1 cut(s) 35
MboI GATC 3 cut(s) 27, 79, 201
MboII GAAGA 4 cut(s) 79, 232, 235, 475
MluCI AATT 1 cut(s) 257
MnlI CCTC 8 cut(s) 40, 43, 95, 166, 184, 240, 289, 325
MslI CAYNNNNRTG 2 cut(s) 381, 452
MwoI GCNNNNNNNGC 3 cut(s) 51, 174, 426
NdeII GATC 3 cut(s) 27, 79, 201
NlaIII CATG 1 cut(s) 380
NlaIV GGNNCC 2 cut(s) 178, 388
NmuCI GTSAC 1 cut(s) 277
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 168
PkrI GCNGC 3 cut(s) 7, 104, 232
Ple19I CGATCG 1 cut(s) 204
Psp1406I AACGTT 1 cut(s) 321
PspEI GGTNACC 1 cut(s) 277
PspN4I GGNNCC 2 cut(s) 178, 388
PspPI GGNCC 3 cut(s) 52, 176, 249
PvuI CGATCG 1 cut(s) 204
RseI CAYNNNNRTG 2 cut(s) 381, 452
SatI GCNGC 3 cut(s) 6, 103, 231
Sau3AI GATC 3 cut(s) 27, 79, 201
Sau96I GGNCC 3 cut(s) 52, 176, 249
SetI ASST 7 cut(s) 87, 115, 126, 195, 300, 324, 441
SfaNI GCATC 2 cut(s) 301, 362
SmiMI CAYNNNNRTG 2 cut(s) 381, 452
Sse9I AATT 1 cut(s) 257
SsiI CCGC 5 cut(s) 5, 35, 102, 180, 188
SspMI CTAG 1 cut(s) 315
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 1 cut(s) 451
TaiI ACGT 2 cut(s) 324, 441
TaqII GACCGA 1 cut(s) 296
TasI AATT 1 cut(s) 257
TauI GCSGC 2 cut(s) 8, 105
TscAI CASTG 1 cut(s) 449
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 1 cut(s) 230
Tsp45I GTSAC 1 cut(s) 277
TspGWI ACGGA 2 cut(s) 105, 148
TspRI CASTG 1 cut(s) 449
XapI RAATTY 1 cut(s) 257
XcmI CCANNNNNNNNNTGG 1 cut(s) 364
XspI CTAG 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.